pycom11g15440

MIP18 family protein At1g68310-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0009043
Physical Location & Seq
Forward (+)
16129810 .. 16131998
2189 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 459 bp
ATGCGAATCCGGTCATCTACGAAAGGAAAGAGCGTCGGGTTCGTAGCGTTCCAAGTGCTGCTGCCGATGAATATGCCGTTGAAGCCATTGACCAACAAGAGATTTTTGCATATTAGAGATATAAAGGACCCGGAACACCCTTATTCTTTGGAGGAGCTCAAAGTAATAACCGAAGATGCAATTGAAGTCGATGATGGCTGTGGCTACGTCAGGGTCACGTTTACTCCTACCGTGGAACATTGTAGTATGGCAACAGTTATTGGTCTTTGCTTACGTGTTAAACTCTTAAGGAGCTTGCCTTCTCGTTACAAAGTGGATATTAGGGTGGCACCAGGATCTCATGCAACCGAAGCTGCCGTTAATAAGCAACTGAATGATAAGGAACGGGTGGCTGCGGCGTTGGAGAACCCAGGCCTCGTTGATATGGTTGATGAATGCCTTTCTCCTTCATACGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FeS_assembly_P

Protein Analysis

153

Amino Acids

16.95

Weight (kDa)

6.9

Isoelectric Point (pI)

43.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FeS_assembly_P PF01883 38 - 105 8.5e-07 Iron-sulfur cluster assembly protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 328
AciI CCGC 1 cut(s) 395
AclWI GGATC 1 cut(s) 343
AflII CTTAAG 1 cut(s) 286
AflIII ACRYGT 1 cut(s) 274
AgsI TTSAA 2 cut(s) 82, 185
AjnI CCWGG 2 cut(s) 331, 409
AluBI AGCT 3 cut(s) 157, 294, 353
AluI AGCT 3 cut(s) 157, 294, 353
Alw21I GWGCWC 1 cut(s) 159
AlwI GGATC 1 cut(s) 343
AoxI GGCC 1 cut(s) 412
ApeKI GCWGC 4 cut(s) 58, 61, 353, 392
AspS9I GGNCC 1 cut(s) 127
AsuC2I CCSGG 1 cut(s) 131
AvaII GGWCC 1 cut(s) 127
BanI GGYRCC 1 cut(s) 328
BanII GRGCYC 1 cut(s) 159
Bbv12I GWGCWC 1 cut(s) 159
BbvI GCAGC 4 cut(s) 45, 48, 340, 379
BccI CCATC 1 cut(s) 188
BceAI ACGGC 2 cut(s) 61, 341
BcgI CGANNNNNNTGC 2 cut(s) 55, 89
BciT130I CCWGG 2 cut(s) 333, 411
BcnI CCSGG 1 cut(s) 131
BfrI CTTAAG 1 cut(s) 286
BisI GCNGC 5 cut(s) 59, 62, 354, 393, 396
BlsI GCNGC 5 cut(s) 60, 63, 355, 394, 397
Bme1390I CCNGG 3 cut(s) 131, 333, 411
Bme18I GGWCC 1 cut(s) 127
BmgT120I GGNCC 1 cut(s) 127
BmiI GGNNCC 2 cut(s) 129, 330
BmrFI CCNGG 3 cut(s) 131, 333, 411
BmsI GCATC 1 cut(s) 166
BpuMI CCSGG 1 cut(s) 131
BsaAI YACGTR 1 cut(s) 275
BsaJI CCNNGG 2 cut(s) 231, 409
BsaWI WCCGGW 1 cut(s) 9
BsaXI ACNNNNNCTCC 2 cut(s) 208, 238
BseBI CCWGG 2 cut(s) 333, 411
BseDI CCNNGG 2 cut(s) 231, 409
BseRI GAGGAG 1 cut(s) 167
BseXI GCAGC 4 cut(s) 45, 48, 340, 379
BshFI GGCC 1 cut(s) 414
BshNI GGYRCC 1 cut(s) 328
BsiHKAI GWGCWC 1 cut(s) 159
BsiSI CCGG 2 cut(s) 10, 131
BsmI GAATGC 1 cut(s) 440
BsnI GGCC 1 cut(s) 414
Bsp1286I GDGCHC 1 cut(s) 159
Bsp143I GATC 1 cut(s) 335
BspACI CCGC 1 cut(s) 395
BspANI GGCC 1 cut(s) 414
BspLI GGNNCC 2 cut(s) 129, 330
BspPI GGATC 1 cut(s) 343
BspT107I GGYRCC 1 cut(s) 328
BspTI CTTAAG 1 cut(s) 286
BssECI CCNNGG 2 cut(s) 231, 409
BssMI GATC 1 cut(s) 335
Bst2UI CCWGG 2 cut(s) 333, 411
Bst4CI ACNGT 2 cut(s) 232, 256
BstAFI CTTAAG 1 cut(s) 286
BstBAI YACGTR 1 cut(s) 275
BstC8I GCNNGC 1 cut(s) 296
BstDSI CCRYGG 1 cut(s) 231
BstKTI GATC 1 cut(s) 338
BstMBI GATC 1 cut(s) 335
BstMWI GCNNNNNNNGC 2 cut(s) 82, 350
BstNI CCWGG 2 cut(s) 333, 411
BstSCI CCNGG 3 cut(s) 129, 331, 409
BstV1I GCAGC 4 cut(s) 45, 48, 340, 379
BstX2I RGATCY 1 cut(s) 335
BstYI RGATCY 1 cut(s) 335
BsuRI GGCC 1 cut(s) 414
BtgI CCRYGG 1 cut(s) 231
Cac8I GCNNGC 1 cut(s) 296
Cfr13I GGNCC 1 cut(s) 127
CseI GACGC 1 cut(s) 22
CviAII CATG 1 cut(s) 341
CviJI RGCY 8 cut(s) 85, 157, 198, 204, 294, 353, 392, 414
CviKI_1 RGCY 8 cut(s) 85, 157, 198, 204, 294, 353, 392, 414
DpnI GATC 1 cut(s) 337
DpnII GATC 1 cut(s) 335
Ecl136II GAGCTC 1 cut(s) 157
Eco147I AGGCCT 1 cut(s) 414
Eco24I GRGCYC 1 cut(s) 159
Eco47I GGWCC 1 cut(s) 127
Eco53kI GAGCTC 1 cut(s) 157
EcoICRI GAGCTC 1 cut(s) 157
EcoO109I RGGNCCY 1 cut(s) 127
EcoRII CCWGG 2 cut(s) 331, 409
EcoT38I GRGCYC 1 cut(s) 159
FaeI CATG 1 cut(s) 344
FaiI YATR 7 cut(s) 74, 111, 122, 248, 342, 425, 451
FatI CATG 1 cut(s) 340
Fnu4HI GCNGC 5 cut(s) 59, 62, 354, 393, 396
FriOI GRGCYC 1 cut(s) 159
Fsp4HI GCNGC 5 cut(s) 59, 62, 354, 393, 396
GluI GCNGC 5 cut(s) 59, 62, 354, 393, 396
HaeIII GGCC 1 cut(s) 414
HapII CCGG 2 cut(s) 10, 131
HgaI GACGC 1 cut(s) 22
Hin1II CATG 1 cut(s) 344
HinfI GANTC 1 cut(s) 6
HpaII CCGG 2 cut(s) 10, 131
Hpy166II GTNNAC 1 cut(s) 222
Hpy8I GTNNAC 1 cut(s) 222
Hpy99I CGWCG 1 cut(s) 38
HpyAV CCTTC 2 cut(s) 309, 456
HpyCH4III ACNGT 2 cut(s) 232, 256
HpyCH4IV ACGT 3 cut(s) 207, 218, 274
HpyCH4V TGCA 3 cut(s) 109, 179, 344
HpyF10VI GCNNNNNNNGC 2 cut(s) 82, 350
HpySE526I ACGT 3 cut(s) 207, 218, 274
Hsp92II CATG 1 cut(s) 344
Kzo9I GATC 1 cut(s) 335
LmnI GCTCC 2 cut(s) 154, 291
LpnPI CCDG 7 cut(s) 23, 144, 196, 318, 345, 396, 423
Lsp1109I GCAGC 4 cut(s) 45, 48, 340, 379
LweI GCATC 1 cut(s) 166
MaeII ACGT 3 cut(s) 207, 218, 274
MaeIII GTNAC 2 cut(s) 214, 305
MalI GATC 1 cut(s) 337
MboI GATC 1 cut(s) 335
MboII GAAGA 1 cut(s) 185
MfeI CAATTG 1 cut(s) 180
MflI RGATCY 1 cut(s) 335
MhlI GDGCHC 1 cut(s) 159
MluCI AATT 1 cut(s) 180
MmeI TCCRAC 1 cut(s) 381
MnlI CCTC 2 cut(s) 145, 425
MseI TTAA 3 cut(s) 279, 287, 360
MslI CAYNNNNRTG 1 cut(s) 454
MspCI CTTAAG 1 cut(s) 286
MspI CCGG 2 cut(s) 10, 131
MspR9I CCNGG 3 cut(s) 131, 333, 411
MunI CAATTG 1 cut(s) 180
Mva1269I GAATGC 1 cut(s) 440
MvaI CCWGG 2 cut(s) 333, 411
MwoI GCNNNNNNNGC 2 cut(s) 82, 350
NciI CCSGG 1 cut(s) 131
NdeII GATC 1 cut(s) 335
NlaIII CATG 1 cut(s) 344
NlaIV GGNNCC 2 cut(s) 129, 330
NmuCI GTSAC 1 cut(s) 214
PceI AGGCCT 1 cut(s) 414
PctI GAATGC 1 cut(s) 440
PfeI GAWTC 1 cut(s) 6
PkrI GCNGC 5 cut(s) 60, 63, 355, 394, 397
Ppu21I YACGTR 1 cut(s) 275
PpuMI RGGWCCY 1 cut(s) 127
Psp124BI GAGCTC 1 cut(s) 159
Psp5II RGGWCCY 1 cut(s) 127
Psp6I CCWGG 2 cut(s) 331, 409
PspGI CCWGG 2 cut(s) 331, 409
PspN4I GGNNCC 2 cut(s) 129, 330
PspPI GGNCC 1 cut(s) 127
PspPPI RGGWCCY 1 cut(s) 127
PsuI RGATCY 1 cut(s) 335
RseI CAYNNNNRTG 1 cut(s) 454
SacI GAGCTC 1 cut(s) 159
SaqAI TTAA 3 cut(s) 279, 287, 360
SatI GCNGC 5 cut(s) 59, 62, 354, 393, 396
Sau3AI GATC 1 cut(s) 335
Sau96I GGNCC 1 cut(s) 127
ScrFI CCNGG 3 cut(s) 131, 333, 411
SduI GDGCHC 1 cut(s) 159
SetI ASST 6 cut(s) 159, 210, 221, 277, 296, 355
SfaNI GCATC 1 cut(s) 166
SinI GGWCC 1 cut(s) 127
SmiMI CAYNNNNRTG 1 cut(s) 454
SmlI CTYRAG 1 cut(s) 286
SmoI CTYRAG 1 cut(s) 286
Sse9I AATT 1 cut(s) 180
SseBI AGGCCT 1 cut(s) 414
SsiI CCGC 1 cut(s) 395
SstI GAGCTC 1 cut(s) 159
StuI AGGCCT 1 cut(s) 414
StyD4I CCNGG 3 cut(s) 129, 331, 409
TaaI ACNGT 2 cut(s) 232, 256
TaiI ACGT 3 cut(s) 210, 221, 277
TaqI TCGA 1 cut(s) 189
TasI AATT 1 cut(s) 180
TauI GCSGC 1 cut(s) 398
TfiI GAWTC 1 cut(s) 6
Tru1I TTAA 3 cut(s) 279, 287, 360
Tru9I TTAA 3 cut(s) 279, 287, 360
TseFI GTSAC 1 cut(s) 214
TseI GCWGC 4 cut(s) 58, 61, 353, 392
Tsp45I GTSAC 1 cut(s) 214
TspDTI ATGAA 3 cut(s) 83, 438, 447
Vha464I CTTAAG 1 cut(s) 286
VpaK11BI GGWCC 1 cut(s) 127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.