pycom11g20140

CCR4-NOT transcription complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0005282
Physical Location & Seq
Reverse (-)
22714936 .. 22734050
19115 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 7086 bp
ATGCTGAAGTTTACGCTGGCCACCGCCAGCCAGATTCGCTTTCTCCTCCAGAGCCTCAACGACGCCAACTCCGACTCCATTCTTCGAGAGGTCTCTCAGTTTATTGAATATGGAATTGAGGGAAGCATTTTGCTGTTACAGGCCTGTCTGGATCATCTCACTAATTACAGAACAGAGTTAAAGAATGCAGCATTGGAGCGGGTTTTTGCCTCAATTTTCAAATCTCTCCTTGATAGACCTAATTTCTGTACGGTGTTTTGTCAGTCCCTAAGAAATACAGAGATTAGTGAAGGAATTCTTGAGAATGTCTCTAATGCATTACACCTGTCTGTATCTGAGAAAACTGGCATTGCTCTTGCGTTGTCAGATTCTGAAAACCTAGACACCAGGATATGTGGCAAGAACTTCTGTATGGCTCAGATCCAGAAATTGTGTGAAAATCCTATTGTTTTGAATTCATCTGAGCAAATTCAGAACATTGTTATGTTCCTCCAGCGGTCTGAGGGCCTTTCCAAGCATGTCGATTCCTTTATGCAGATGTTATCTTTACTTCAGCTGAAAGATGACAGTTTTTCTGTTTTAACTCCATTGCTTTCGGATGAGCTCCGTGATGCCAATTTTTTGAGCAATGTGGATTTGTTGCATGAAAATGGAGAAAATAATTTTGATGCTATTTTATCTGAAATGGAGAAGGAAATCAGCATGAGAGATATAATGAAGGAATTAGGTTATGGATGCACAGTTGATTCATCTCAGTGCAGCGAGATTTTATCTCTTTTCTTACCTCTCACTGAGATTACTATTTCAAAAATCCTTGGCATGATTGCCTCTACTCATGTTGGTCTTGAGGACAATCAGAATACATTTTCAACGTTCTGGTTGGCGCTTGGTAACAGCACTTTATCCGATCTGCCTATGTTGAACACCTGGAATATTGATGTCCTTGTAGATACAATCAAGCAACTTGCTCCCGGCACTAACTGGATACAAGTTATTGAAAATTTGGATCATGAGGGATTCTACATTCCTAATCAGGAGGCTTTCTCTTTTTTCATGTCTGTGTATCAGCATGTGTGTCAGGATCCAATCCCCCTCCATGTTATCTGCGGGTCTGTTTGGAAGAATACCGAGGGTCAGTTATCCTTCCTTAAACATGCTATATCAGCACCACCTGAAGTGTTTACCTTTGCCCACTCTGTTAGGCAACTGGCCTATGTTGATGTAGTGCATGGCCATAAGCTTCAACTTGGACATGCAAATCATGCATGGTTGAGTCTTGACCTTTTGGATGTACTGTGTCTACTAGCTGAGAGGGGTCATGCTCTTGCTGTTCGATCAATGCTTGAGTATCCTCTTAAACACTGTCCTGAAGTCCTGCTTCTTGGGATGGCACATACAAATACTGTGTATAACCTGCTGCAATATGAAGTGTCTTATACTGTTTTCCCCATGATGGTAAAAAATGCAACGGGCAGTGGCATAATTAACCACCTCTGGCATATTAACACCTCATTGGTGCTGCGGGGATTTGTGGATGCTCACAATTGTGATCCAGATAACATAGCTAGAATATTGGATATCTGTCAAGAGCTAAAGATTCTATCATCTGTCCTAGAGATGATCCCTTCCTCTTTTAGTATCAGAATGGCAGCCCTTGCTTCCAGAAAAGAGCTTGTAGACCTTGAGAAGTGGTTGAGCAATAATTTGAATACGCATAAGGATATTTTCTTTGAGGAGTGCATAAAGTTCTTGAAGGAGATTCAATTCGGTGGGTCACAGGATTTCTCCACCAGACCTTTCCAACATTCTGGTGCCGTGTCTAATATTTATGTGGACGCTGCTACTATATTCTTGAAGGTTCTTAAAGCTTATATTGGCTTGATTGCCTCTAGCCAACTTACTGAGGAGTTGGAAAGGTTGTCTGTAACAATTATGGATTCTAATCCAAGGCTGCAGAATGGTGACCCTACAGAGTCAACCGATGGATATGCAGATGATATTGAAGCTGAAGCAAACTCTTACTTTCATCAAATGTTTTCTGGTCAGTTGACAATTGATTCAATGGTCCAAATGCTTGCCCGATTCAAGGAATCTTCTATAAAAAGGGAACAGTCAATTTTTGAGTGCATGATTGCAAATCTGTTTGAAGAATATAGATTTTTCCCCAAGTATCCTGAAAGACAGCTCAAAATTGCCGCAGTTCTGTTTGGCTCTGTCATCAAACACCAGCTTGTAACTCATCTGACTCTTGGGATCGCTCTGCGTGGTGTTTTAGATGCATTGCGCAAACCTGCGGATTCAAAAATGTTTGTGTTCGGAACTAAGGCCTTGGAGCAGTTTGTGGATCGTCTGATTGAGTGGCCTCAGTATTGCAATCATATTCTTCAAATATCCCATCTGCGGAGTACTCATTCAGAGCTTGTGGCTTTCATTGAACAGGCTCTTGCCAGGATTTCATTAGGCCATTTAGACTCAGATGGAAGTAACGATGCTTCCGCTGCTCATCACCATGGTCCCAGTCAAGGTACCCCAGGAAATGTAGAGTTAAATGGCTCTACCATCTTACATACTGGGCAACAACTTTCTTCACCACTCCAGCTTCAACAGAGACATGAAAGTTCTCTTGATGACCATCATAAAGCCGCAAATTCATCAAATGACATAAAGCCATCTTTATCTTCTGCTGTGCATCCTTCGGTTGGTCCTGTTGACGATCCTCCTAGTATTCAGAAGTCACAAAGTGCACTCAGTGCTCCAGTAATGTTGTCTTCTGCTTCCTCTGGTTTTGTTCGTCCTTCTCGAGGAGTTACTTCCACCAGGTTTGGCTCTGCTTTGAATATTGAAACATTAGTTGCTGCTGCCGAGAAAAGAGATACTCCCATCGAGGCTCCAGCATCAGAGATTCAGGACAAGATATCATTTATAATTAATAATATTTCAGTTGCCAATGTTGAAGCTAAGTCAAAGGAGTTTGCTGAAATAATGAAAGAGCAGTACCATCCTTGGTTTGCACAGTATATGGTTATGAAAAGGGCAAGCATTGAGCCGAATTTTCATGATTTGTACCTGAAATTTCTGGACAAAGTTAATTCTAAGGGGTTGAATAAGGAGATTGTTCAAGCCTCTTACGAGAACTGCAAGGTTCTTCTTGGATCTGAGCTTATAAAATCAAGCTCAGAAGAGCGTTCATTGTTAAAAAATTTAGGCAGTTGGCTTGGGAAGCTTACAATTGGAAGGAATCAAGTCTTAAGGGCTCGGGAAATAGATCCCAAATCTTTGATTATAGAGGCGTATGAAAAGGGGTTGATGATTGCAGTTATACCCTTTACTTCAAAGATTCTGGAGCCATGCCAAAGTAGCTTGGCATATCAGCCACCAAATCCTTGGACAATGGGTATTCTTGGATTACTTGCTGAGATATATTCAATGCCAAACTTGAAAATGAACCTGAAGTTTGATATTGAGGTTCTATTCAAGAATCTTGGTGTGGATTTGAAGGAGATAACCCCATGTTCTCTTCTCAAGGACCGGAACAGAGAACTTGAAGGGAATCCTGATTTCTCTAACAAAGATATTGGATCATCCCAACCACAGTTGGTTGCTGATGTGAAATCCGGAATGTTATCTCCACTAAATCAGGTTGAACTACCCGTTGAGGTTGCTCCATCTAATTCTGGAAGCCATACACATCTACTATCTCAGGTTCCCACACCAATATCTAATATTGCAACTCACGTGGTTATCAACCCGAAACTCAGTGCTTTGGGATTGCACTTGCATTTTCAGAGGGTGGTTCCGATTGCAATGGACAGAGCTATCAAAGAGATAGTATCTGGTATTGTTCAGCGCAGTGTTTCTATAGCTACTCAGACAACTAAAGAACTTGTCTTAAAGGATTATGCCATGGAATCCGATGAGACAAGAATATTATATGCAGCTCACTTGATGGTTGCAAGTTTGGCAGGAAGTCTAGCTCATGTAACTTGCAAGGAACCCTTGCGTACTTCAATATCAACTCAGCTGCGAAATTCCCTTCAGGGATCAAACATTCCAAATGATCATCTGGAACATGCTGTACAACTTGTTACTAATGACAATCTGGATCTTGGCTGTGCAATCATTGAACAGGCTGCTACTGATAAGGCGGTACAAACAATTGATGGAGAAATAGCTCAACAACTGTCATTAAGAAGGAAGAGGGAAGGTGCGGGTACAACATTTTTTGATGCTAACATGTACACACAAAGTGGCAGTGTGCCCGAGTCCCTTCGCCCTAAACCTGGTCATTTGTCCATTTCTCAACAACGAGTTTATGAGGACTTCGTGCGGCTTCCCTGGCAAAACCAGTCTAGCCAGAATTCACATGTTCTTCCTGCTAGTACTGCAGCTCCTTCTGCAAGTGCTGGTCTAAATGGCACATTTGGTTCAGCCTCGGTTCAACTTAACACAGGCTACTCAGCAGGTCCTGGAAGCAAGTTTGATGCTGTTTCTCGTCCACTGGATGAGGCAGTTGAACCCAATCCAGCTCTGCATCCAAGTTCATCCTCTATTTGTGTTGGAGCGGGTGATGGTGTTTCCCAGCATAGTTCTGAGAATGATTCTGTCATTGGTTCATTTCTTTCTGCTGCTTCTGCCCCAGAGCTGCAGTCAGTAGAGTCATCTGATTTTATAAAGGAATCTGGAATTTCTTCACAGCCGCTGCCTTCATCTACTGCAACTGAACGTCTGGCATGCAACATCTCAGAACCTTCTCTCAATACGAGGGATGCATTGGATAAATACCAGATTGTTTCACAGAAGCTTGAAGCTTTGGTAACCAACGATGCTAAAGAGTCAGAAATTCAGGGAGTAATTGGTGAAGTTCCTGAGATTATACTCAGATGTGTTAGTCGAGATGAGGCTGCCTTGGCTGTGGCTCAAAAGGTTTTCAAGGGTTTATACGAGAATGCATCGAACCATACTCACGTCAGTGCTCATATTGCAATGCTGACAGCTATTCGCGAAGTTTGCAAGCTTGTTGTTAAGGAGCTCACCAGTTGGGTTATTTATTCTGATGAAGAGCGGAAGTTTAACAAAGATATTACCGTTGGCCTTATTCACAGTGAATTACTAAATCTTGCAGAGTACAATGTTCAAATGGCAAAACTCATTGATGGGGGCAGAAATAAGCTTGCAACTGAGTTTTCAATTTCCCTTCTCCAATCTTTGGTGATTGAAGAATCTAAAGTTATATCAGAACTTCAAAATCTTGTTGACGCATTGGCAAAGCTCGCTGCAAAGCCTGGATTTCCTGAGTCGCTACAGCAGCTGGTTGAAATGGTAAAGTATCCTGCTTCTATAGTGGGTGCTCCGTCTGCTATTAATGTAGGAAAAGAGGACAAGGGCAGAATATCTAAGGATAAAAAGGCTTCTGTTCACTCTCTGGTGAGCAGGGAAGACTTTAGTAATGTGGAGTCTGTCGAACCAGATCCTCCTGGATTCCGTGAGCAGGTGTCCATGCTGTTTGCAGAGTGGTACCGGATTTGTGAACTTCCTGGTGCAAACGATGCAGCTTATACCCATGTTATCTTGCAGCTGCATCAAAATGGTCTGCTGAAAGGGGATGATATGACAGACCGGTTTTTCCGTGTCCTCACAGAGCTATCTGTTGCACATTGCCTATCTTCTGAGATGACAATTCCAGGGACACTGCAAGCACCTCAACAAGTGCCGAATTTCTCCTTCCTTGCTATTGATATCTATGCTAAACTTGTTTTCTTAATTTTGAAGGGATCAAATAAACTATTTCTCTTGTCTAAGATTTTAGCAGTCACAGTGAGATTCATTCAAAAGGATGCAGAGGAGAAAAAAGGATCTTTCAACCCAAGACCGTATTTTAGATTGATTGTTAACTGGCTTCTAGACCTTGGATCTTTGGATCCTGTGATTGATGGTGCTAACTTTCAGATATTGACAGCATTTGCAAATGCATTTCATGCTTTGCAGCCCCTTAAGGTTCCTACATTCAGCTTTGCATGGCTTGAGTTGGTGAGTCATAGGAGTTTCATGCCAAAAATGCTTGCTGGAAATGGCCAGAAGGGTTGGCCCTATATCCAACGTTTGCTGGTACATTTGTTTCAATTCATGGAGCCATTTTTGAGGAATGCTGAACTTGGAGTCCCGGTTCATTTCCTGTATAAAGGCACACTTAGGGTGCTGCTAGTGCTACTCCATGACTTTCCGGAGTTCCTTTGTGATTATCATTTTACGTTCTGTGATGTAATTCCTCCAAGCTGCATTCAAATGCGAAATATCATCCTCAGTGCGTTTCCCCGTAATATGAGGCTACCAGATCGATCATCTACTCCCAACTTAAAGATTGATTTACTTGCTGAAATCAGTCAGTCTCCACGTATTCTCTCTGAGGTTGATGCAGCTCTAAAAGCGAAGCAGATGAAAGCTGATGTGGATGAGTACCTCAAGACAAGGCAACAGGGTTCTTCATTTCTGACTGAACTGAAGCAAAAGTTGCTCCTTTTACCAAGCGAAGCTGCCTCAGCTGGTACCCGTTACAATGTACCCCTGATCAACTCCCTTGTGCTTTATGTTGGGATGCAGGCTATCCAGCAGCTTCAGGCTAGAACGCCTCAAGCCCAATCTACCCAAACTGTTCCATTGGCTGTCTATTTGGTGGGTGCTGCTTTGGATATTTTTCAGACTCTGATAGTGGACCTAGATACTGAAGGGCGCTACCTTTTCCTAAATGCGATTGCGAACCAACTGCGTTATCCAAACACCCATACCCATTACTTTTCCTTCATTGTTCTGTACTTGTTTGCCGATTCAAACCAGCATGAAATTATCCAGGAGCAAATCACAAGGGTATTGTTGGAACGTCTGATTGTTAACCGACCTCATCCATGGGGTCTTCTGATTACCTTCATTGAGCTTATCAAGAATCCGAGATACCAGTTCTGGAACCGAGCATTCATCAGATGTGCACCGGAGATTGAAAAACTTTTCGAATCTGTCTCAAGGTCTTGCGGCGGTCCAAAACCTGTGGACGAAAGTATGGTCTCAGGTTGGGTATCAGAGAGTGCTCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
CNOT1_CAF1_bind CNOT1_HEAT CNOT1_TTP_bind DUF3819 Not1

Protein Analysis

2362

Amino Acids

262.12

Weight (kDa)

5.71

Isoelectric Point (pI)

41.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CNOT1_HEAT PF16418 476 - 622 5.7e-37 CCR4-NOT transcription complex subunit 1 HEAT repeat
CNOT1_TTP_bind PF16417 657 - 816 1.4e-67 CCR4-NOT transcription complex subunit 1 TTP binding domain
CNOT1_CAF1_bind PF16415 961 - 1180 3.1e-89 CCR4-NOT transcription complex subunit 1 CAF1-binding domain
DUF3819 PF12842 1260 - 1399 5.5e-50 CCR4-Not complex, Not1 subunit, domain of unknown function DUF3819
ARM_Cnot1 PF25097 1601 - 1775 7.7e-54 Cnot1 ARM repeat
NOT1_connector PF23590 1624 - 1774 7.6e-17 CCR4-NOT transcription complex subunit 1, NOT1_connector
Not1 PF04054 1975 - 2337 4.7e-139 CCR4-Not complex component, Not1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 2924, 3164, 4670
AarI CACCTGC 1 cut(s) 5479
Acc16I TGCGCA 1 cut(s) 2285
Acc36I ACCTGC 4 cut(s) 1422, 2299, 4451, 5479
Acc65I GGTACC 3 cut(s) 2525, 5514, 6542
AccB1I GGYRCC 4 cut(s) 1811, 2525, 5514, 6542
AccB7I CCANNNNNTGG 1 cut(s) 5206
AccBSI CCGCTC 3 cut(s) 199, 4562, 5062
AccI GTMKAC 2 cut(s) 1300, 1677
AccII CGCG 1 cut(s) 5001
AccIII TCCGGA 2 cut(s) 3614, 6220
AclI AACGTT 2 cut(s) 872, 6097
AcoI YGGCCR 3 cut(s) 18, 1231, 6070
AcvI CACGTG 1 cut(s) 3736
AcyI GRCGYC 1 cut(s) 63
AdeI CACNNNGTG 3 cut(s) 2741, 2750, 4247
AfiI CCNNNNNNNGG 9 cut(s) 1453, 2086, 2400, 2522, 2699, 2801, 4280, 5206, 5488
AflII CTTAAG 2 cut(s) 3247, 5990
AflIII ACRYGT 2 cut(s) 4233, 4363
AgeI ACCGGT 1 cut(s) 5616
AjiI CACGTC 1 cut(s) 4966
AjuI GAANNNNNNNTTGG 2 cut(s) 176, 208
AleI CACNNNNGTG 2 cut(s) 1545, 4968
AloI GAACNNNNNNTCC 4 cut(s) 6141, 6147, 6173, 6179
Alw21I GWGCWC 8 cut(s) 606, 2746, 2755, 4975, 5031, 5350, 6982, 7081
Alw26I GTCTC 7 cut(s) 97, 313, 2602, 3911, 6390, 7015, 7060
Alw44I GTGCAC 2 cut(s) 2742, 6978
AlwNI CAGNNNCTG 5 cut(s) 371, 4466, 4699, 5308, 6700
Ama87I CYCGRG 3 cut(s) 2799, 3255, 4259
Aor13HI TCCGGA 2 cut(s) 3614, 6220
ApaLI GTGCAC 2 cut(s) 2742, 6978
ArsI GACNNNNNNTTYG 2 cut(s) 7038, 7070
AseI ATTAAT 2 cut(s) 2928, 5361
AsiGI ACCGGT 1 cut(s) 5616
Asp700I GAANNNNTTC 2 cut(s) 294, 4687
Asp718I GGTACC 3 cut(s) 2525, 5514, 6542
AspLEI GCGC 4 cut(s) 886, 2286, 3849, 6729
AspS9I GGNCC 9 cut(s) 505, 2065, 2513, 2702, 3526, 4463, 6084, 6709, 7028
AsuC2I CCSGG 2 cut(s) 972, 6161
AsuHPI GGTGA 9 cut(s) 1973, 2498, 2580, 4577, 4868, 5023, 5221, 5437, 6040
AsuII TTCGAA 1 cut(s) 7002
AvaI CYCGRG 3 cut(s) 2799, 3255, 4259
AvaII GGWCC 7 cut(s) 2065, 2513, 2702, 3526, 4463, 6709, 7028
BaeGI GKGCMC 3 cut(s) 2746, 4260, 6982
BaeI ACNNNNGTAYC 2 cut(s) 6098, 6131
BalI TGGCCA 3 cut(s) 20, 1233, 6072
BamHI GGATCC 2 cut(s) 1081, 5917
BanI GGYRCC 4 cut(s) 1811, 2525, 5514, 6542
BanII GRGCYC 3 cut(s) 606, 3256, 5031
BarI GAAGNNNNNNTAC 2 cut(s) 2476, 2508
BbrPI CACGTG 1 cut(s) 3736
BbsI GAAGAC 3 cut(s) 2760, 5442, 6899
Bbv12I GWGCWC 8 cut(s) 606, 2746, 2755, 4975, 5031, 5350, 6982, 7081
BbvCI CCTCAGC 1 cut(s) 6535
BceAI ACGGC 1 cut(s) 1799
BcgI CGANNNNNNTGC 4 cut(s) 1970, 2004, 6742, 6776
BciVI GTATCC 4 cut(s) 978, 1359, 2181, 5337
BclI TGATCA 2 cut(s) 4057, 6564
BcnI CCSGG 2 cut(s) 972, 6161
BcoDI GTCTC 7 cut(s) 97, 313, 2602, 3911, 6390, 7015, 7060
BfmI CTRYAG 7 cut(s) 1952, 1968, 3858, 4383, 4643, 5300, 5337
BfoI RGCGCY 2 cut(s) 887, 6730
BfrI CTTAAG 2 cut(s) 3247, 5990
BfuAI ACCTGC 4 cut(s) 1422, 2299, 4451, 5479
BfuI GTATCC 4 cut(s) 978, 1359, 2181, 5337
BmcAI AGTACT 2 cut(s) 2407, 4381
Bme18I GGWCC 7 cut(s) 2065, 2513, 2702, 3526, 4463, 6709, 7028
BmeT110I CYCGRG 3 cut(s) 2799, 3255, 4259
BmgBI CACGTC 1 cut(s) 4966
BmgT120I GGNCC 9 cut(s) 505, 2065, 2513, 2702, 3526, 4463, 6084, 6709, 7028
BmrI ACTGGG 2 cut(s) 2511, 2580
BmuI ACTGGG 2 cut(s) 2511, 2580
BpiI GAAGAC 3 cut(s) 2760, 5442, 6899
BplI GAGNNNNNCTC 4 cut(s) 293, 325, 1028, 1060
BpmI CTGGAG 6 cut(s) 32, 476, 2579, 2739, 2874, 3362
Bpu10I CCTNAGC 1 cut(s) 6535
Bpu14I TTCGAA 1 cut(s) 7002
BpuEI CTTGAG 9 cut(s) 320, 866, 1364, 1703, 3506, 6041, 6443, 6612, 6997
BpuMI CCSGG 2 cut(s) 972, 6161
Bsa29I ATCGAT 1 cut(s) 6334
BsaAI YACGTR 2 cut(s) 3736, 6392
BsaBI GATNNNNATC 2 cut(s) 1941, 2631
BsaHI GRCGYC 1 cut(s) 63
BsaI GGTCTC 2 cut(s) 97, 7060
BsaWI WCCGGW 6 cut(s) 3528, 3614, 5517, 5616, 6220, 6982
BsaXI ACNNNNNCTCC 8 cut(s) 53, 59, 83, 89, 4372, 4402, 6147, 6177
Bsc4I CCNNNNNNNGG 9 cut(s) 1453, 2086, 2400, 2522, 2699, 2801, 4280, 5206, 5488
Bse118I RCCGGY 1 cut(s) 5616
Bse3DI GCAATG 7 cut(s) 348, 587, 634, 2279, 3810, 4989, 5653
Bse8I GATNNNNATC 2 cut(s) 1941, 2631
BseAI TCCGGA 2 cut(s) 3614, 6220
BseCI ATCGAT 1 cut(s) 6334
BseJI GATNNNNATC 2 cut(s) 1941, 2631
BseLI CCNNNNNNNGG 9 cut(s) 1453, 2086, 2400, 2522, 2699, 2801, 4280, 5206, 5488
BseMI GCAATG 7 cut(s) 348, 587, 634, 2279, 3810, 4989, 5653
BseRI GAGGAG 5 cut(s) 35, 1748, 1919, 2817, 5855
BseSI GKGCMC 3 cut(s) 2746, 4260, 6982
BseYI CCCAGC 1 cut(s) 4578
BsgI GTGCAG 1 cut(s) 778
Bsh1236I CGCG 1 cut(s) 5001
BshNI GGYRCC 4 cut(s) 1811, 2525, 5514, 6542
BshTI ACCGGT 1 cut(s) 5616
BshVI ATCGAT 1 cut(s) 6334
BsiHKAI GWGCWC 8 cut(s) 606, 2746, 2755, 4975, 5031, 5350, 6982, 7081
BsiHKCI CYCGRG 3 cut(s) 2799, 3255, 4259
BsiSI CCGG 8 cut(s) 972, 3529, 3615, 5518, 5617, 6161, 6221, 6983
BslFI GGGAC 5 cut(s) 250, 2499, 4249, 5698, 6143
BslI CCNNNNNNNGG 9 cut(s) 1453, 2086, 2400, 2522, 2699, 2801, 4280, 5206, 5488
BsmAI GTCTC 7 cut(s) 97, 313, 2602, 3911, 6390, 7015, 7060
BsmFI GGGAC 5 cut(s) 250, 2499, 4249, 5698, 6143
BsmI GAATGC 5 cut(s) 190, 4951, 6148, 6276, 6965
Bso31I GGTCTC 2 cut(s) 97, 7060
BsoBI CYCGRG 3 cut(s) 2799, 3255, 4259
Bsp119I TTCGAA 1 cut(s) 7002
Bsp13I TCCGGA 2 cut(s) 3614, 6220
Bsp1407I TGTACA 2 cut(s) 4075, 4236
Bsp19I CCATGG 3 cut(s) 2509, 3903, 6899
Bsp68I TCGCGA 1 cut(s) 5001
BspDI ATCGAT 1 cut(s) 6334
BspEI TCCGGA 2 cut(s) 3614, 6220
BspFNI CGCG 1 cut(s) 5001
BspHI TCATGA 2 cut(s) 1009, 3055
BspMAI CTGCAG 3 cut(s) 1956, 4387, 4647
BspMI ACCTGC 4 cut(s) 1422, 2299, 4451, 5479
BspQI GCTCTTC 2 cut(s) 3174, 5052
BspT104I TTCGAA 1 cut(s) 7002
BspT107I GGYRCC 4 cut(s) 1811, 2525, 5514, 6542
BspTI CTTAAG 2 cut(s) 3247, 5990
BspTNI GGTCTC 2 cut(s) 97, 7060
BsrBI CCGCTC 3 cut(s) 199, 4562, 5062
BsrDI GCAATG 7 cut(s) 348, 587, 634, 2279, 3810, 4989, 5653
BsrFI RCCGGY 1 cut(s) 5616
BsrGI TGTACA 2 cut(s) 4075, 4236
BssAI RCCGGY 1 cut(s) 5616
BssNI GRCGYC 1 cut(s) 63
Bst6I CTCTTC 4 cut(s) 3174, 3522, 4190, 5052
BstACI GRCGYC 1 cut(s) 63
BstAFI CTTAAG 2 cut(s) 3247, 5990
BstAPI GCANNNNNTGC 6 cut(s) 1262, 1655, 2750, 5546, 5975, 6508
BstAUI TGTACA 2 cut(s) 4075, 4236
BstBAI YACGTR 2 cut(s) 3736, 6392
BstBI TTCGAA 1 cut(s) 7002
BstDSI CCRYGG 3 cut(s) 2509, 3903, 6899
BstEII GGTNACC 2 cut(s) 1961, 4813
BstENI CCTNNNNNAGG 1 cut(s) 2799
BstFNI CGCG 1 cut(s) 5001
BstH2I RGCGCY 2 cut(s) 887, 6730
BstHHI GCGC 4 cut(s) 886, 2286, 3849, 6729
BstMAI GTCTC 7 cut(s) 97, 313, 2602, 3911, 6390, 7015, 7060
BstNSI RCATGY 8 cut(s) 521, 1073, 1157, 1256, 4073, 4237, 4367, 4734
BstPI GGTNACC 2 cut(s) 1961, 4813
BstSFI CTRYAG 7 cut(s) 1952, 1968, 3858, 4383, 4643, 5300, 5337
BstSLI GKGCMC 3 cut(s) 2746, 4260, 6982
BstUI CGCG 1 cut(s) 5001
BstV2I GAAGAC 3 cut(s) 2760, 5442, 6899
BstX2I RGATCY 9 cut(s) 420, 1081, 3152, 3265, 4102, 5467, 5852, 5909, 5917
BstXI CCANNNNNNTGG 2 cut(s) 1808, 3384
BstYI RGATCY 9 cut(s) 420, 1081, 3152, 3265, 4102, 5467, 5852, 5909, 5917
Bsu15I ATCGAT 1 cut(s) 6334
BsuI GTATCC 4 cut(s) 978, 1359, 2181, 5337
BsuTUI ATCGAT 1 cut(s) 6334
BtgI CCRYGG 3 cut(s) 2509, 3903, 6899
BtrI CACGTC 1 cut(s) 4966
BtsI GCAGTG 4 cut(s) 1480, 3856, 4258, 5687
BtuMI TCGCGA 1 cut(s) 5001
BveI ACCTGC 4 cut(s) 1422, 2299, 4451, 5479
CaiI CAGNNNCTG 5 cut(s) 371, 4466, 4699, 5308, 6700
CciI TCATGA 2 cut(s) 1009, 3055
CfoI GCGC 4 cut(s) 886, 2286, 3849, 6729
Cfr10I RCCGGY 1 cut(s) 5616
Cfr13I GGNCC 9 cut(s) 505, 2065, 2513, 2702, 3526, 4463, 6084, 6709, 7028
ClaI ATCGAT 1 cut(s) 6334
CseI GACGC 3 cut(s) 71, 1844, 5264
CsiI ACCWGGT 2 cut(s) 2816, 4279
CspAI ACCGGT 1 cut(s) 5616
CspCI CAANNNNNGTGG 6 cut(s) 2803, 2838, 3717, 3752, 7020, 7055
DraIII CACNNNGTG 3 cut(s) 2741, 2750, 4247
EaeI YGGCCR 3 cut(s) 18, 1231, 6070
Eam1104I CTCTTC 4 cut(s) 3174, 3522, 4190, 5052
EarI CTCTTC 4 cut(s) 3174, 3522, 4190, 5052
Ecl136II GAGCTC 2 cut(s) 604, 5029
Eco147I AGGCCT 2 cut(s) 143, 2327
Eco24I GRGCYC 3 cut(s) 606, 3256, 5031
Eco31I GGTCTC 2 cut(s) 97, 7060
Eco32I GATATC 3 cut(s) 1579, 2916, 5737
Eco47I GGWCC 7 cut(s) 2065, 2513, 2702, 3526, 4463, 6709, 7028
Eco53kI GAGCTC 2 cut(s) 604, 5029
Eco72I CACGTG 1 cut(s) 3736
Eco88I CYCGRG 3 cut(s) 2799, 3255, 4259
Eco91I GGTNACC 2 cut(s) 1961, 4813
EcoICRI GAGCTC 2 cut(s) 604, 5029
EcoNI CCTNNNNNAGG 1 cut(s) 2799
EcoO109I RGGNCCY 2 cut(s) 505, 4463
EcoO65I GGTNACC 2 cut(s) 1961, 4813
EcoRI GAATTC 3 cut(s) 294, 454, 4357
EcoRV GATATC 3 cut(s) 1579, 2916, 5737
EcoT22I ATGCAT 6 cut(s) 319, 1267, 2281, 4771, 4951, 5971
EcoT38I GRGCYC 3 cut(s) 606, 3256, 5031
FalI AAGNNNNNCTT 8 cut(s) 282, 314, 1362, 1394, 3980, 4012, 6171, 6203
FaqI GGGAC 5 cut(s) 250, 2499, 4249, 5698, 6143
FauI CCCGC 5 cut(s) 192, 1100, 1515, 4201, 4555
FbaI TGATCA 2 cut(s) 4057, 6564
FblI GTMKAC 2 cut(s) 1300, 1677
FriOI GRGCYC 3 cut(s) 606, 3256, 5031
FspI TGCGCA 1 cut(s) 2285
GlaI GCGC 4 cut(s) 885, 2285, 3848, 6728
GsaI CCCAGC 1 cut(s) 4582
GsuI CTGGAG 6 cut(s) 32, 476, 2579, 2739, 2874, 3362
HaeII RGCGCY 2 cut(s) 887, 6730
HapII CCGG 8 cut(s) 972, 3529, 3615, 5518, 5617, 6161, 6221, 6983
HgaI GACGC 3 cut(s) 71, 1844, 5264
HhaI GCGC 4 cut(s) 886, 2286, 3849, 6729
Hin1I GRCGYC 1 cut(s) 63
Hin6I GCGC 4 cut(s) 884, 2284, 3847, 6727
HinP1I GCGC 4 cut(s) 884, 2284, 3847, 6727
HincII GTYRAC 6 cut(s) 1977, 2049, 2710, 5254, 5890, 6886
HindII GTYRAC 6 cut(s) 1977, 2049, 2710, 5254, 5890, 6886
HindIII AAGCTT 7 cut(s) 1238, 1866, 3221, 4799, 4806, 5012, 5168
HpaI GTTAAC 2 cut(s) 5890, 6886
HpaII CCGG 8 cut(s) 972, 3529, 3615, 5518, 5617, 6161, 6221, 6983
HphI GGTGA 9 cut(s) 1973, 2498, 2580, 4577, 4868, 5023, 5221, 5437, 6040
Hpy99I CGWCG 1 cut(s) 65
HpyCH4IV ACGT 8 cut(s) 872, 3735, 4723, 4965, 6097, 6248, 6391, 6874
HpySE526I ACGT 8 cut(s) 872, 3735, 4723, 4965, 6097, 6248, 6391, 6874
Hsp92I GRCGYC 1 cut(s) 63
HspAI GCGC 4 cut(s) 884, 2284, 3847, 6727
Kpn2I TCCGGA 2 cut(s) 3614, 6220
KpnI GGTACC 3 cut(s) 2529, 5518, 6546
Ksp22I TGATCA 2 cut(s) 4057, 6564
KspAI GTTAAC 2 cut(s) 5890, 6886
LguI GCTCTTC 2 cut(s) 3174, 5052
MabI ACCWGGT 2 cut(s) 2816, 4279
MaeII ACGT 8 cut(s) 872, 3735, 4723, 4965, 6097, 6248, 6391, 6874
MbiI CCGCTC 3 cut(s) 199, 4562, 5062
MfeI CAATTG 4 cut(s) 1543, 2052, 3228, 4155
MflI RGATCY 9 cut(s) 420, 1081, 3152, 3265, 4102, 5467, 5852, 5909, 5917
MlsI TGGCCA 3 cut(s) 20, 1233, 6072
MluNI TGGCCA 3 cut(s) 20, 1233, 6072
MmeI TCCRAC 6 cut(s) 96, 1825, 1890, 4537, 6118, 6849
Mox20I TGGCCA 3 cut(s) 20, 1233, 6072
Mph1103I ATGCAT 6 cut(s) 319, 1267, 2281, 4771, 4951, 5971
MroI TCCGGA 2 cut(s) 3614, 6220
MroXI GAANNNNTTC 2 cut(s) 294, 4687
MscI TGGCCA 3 cut(s) 20, 1233, 6072
Msp20I TGGCCA 3 cut(s) 20, 1233, 6072
MspA1I CMGCKG 8 cut(s) 496, 556, 2498, 4021, 4699, 5308, 5575, 6539
MspCI CTTAAG 2 cut(s) 3247, 5990
MspI CCGG 8 cut(s) 972, 3529, 3615, 5518, 5617, 6161, 6221, 6983
MunI CAATTG 4 cut(s) 1543, 2052, 3228, 4155
Mva1269I GAATGC 5 cut(s) 190, 4951, 6148, 6276, 6965
MvnI CGCG 1 cut(s) 5001
NciI CCSGG 2 cut(s) 972, 6161
NcoI CCATGG 3 cut(s) 2509, 3903, 6899
NmeAIII GCCGAG 1 cut(s) 2887
NmuCI GTSAC 4 cut(s) 1773, 1961, 2733, 5809
NruI TCGCGA 1 cut(s) 5001
NsbI TGCGCA 1 cut(s) 2285
NsiI ATGCAT 6 cut(s) 319, 1267, 2281, 4771, 4951, 5971
NspI RCATGY 8 cut(s) 521, 1073, 1157, 1256, 4073, 4237, 4367, 4734
NspV TTCGAA 1 cut(s) 7002
OliI CACNNNNGTG 2 cut(s) 1545, 4968
PaeI GCATGC 1 cut(s) 4734
PaeR7I CTCGAG 1 cut(s) 2799
PagI TCATGA 2 cut(s) 1009, 3055
PaqCI CACCTGC 1 cut(s) 5479
PceI AGGCCT 2 cut(s) 143, 2327
PciI ACATGT 2 cut(s) 4233, 4363
PciSI GCTCTTC 2 cut(s) 3174, 5052
PcsI WCGNNNNNNNCGW 2 cut(s) 69, 2797
PctI GAATGC 5 cut(s) 190, 4951, 6148, 6276, 6965
PdmI GAANNNNTTC 2 cut(s) 294, 4687
PflMI CCANNNNNTGG 1 cut(s) 5206
PfoI TCCNGGA 3 cut(s) 4465, 5473, 6843
PinAI ACCGGT 1 cut(s) 5616
PmaCI CACGTG 1 cut(s) 3736
PmlI CACGTG 1 cut(s) 3736
Ppu21I YACGTR 2 cut(s) 3736, 6392
PpuMI RGGWCCY 1 cut(s) 4463
PscI ACATGT 2 cut(s) 4233, 4363
PshBI ATTAAT 2 cut(s) 2928, 5361
PsiI TTATAA 3 cut(s) 2924, 3164, 4670
Psp124BI GAGCTC 2 cut(s) 606, 5031
Psp1406I AACGTT 2 cut(s) 872, 6097
Psp5II RGGWCCY 1 cut(s) 4463
PspCI CACGTG 1 cut(s) 3736
PspEI GGTNACC 2 cut(s) 1961, 4813
PspFI CCCAGC 1 cut(s) 4578
PspPI GGNCC 9 cut(s) 505, 2065, 2513, 2702, 3526, 4463, 6084, 6709, 7028
PspPPI RGGWCCY 1 cut(s) 4463
PsrI GAACNNNNNNTAC 2 cut(s) 4059, 4091
PstI CTGCAG 3 cut(s) 1956, 4387, 4647
PstNI CAGNNNCTG 5 cut(s) 371, 4466, 4699, 5308, 6700
PsuI RGATCY 9 cut(s) 420, 1081, 3152, 3265, 4102, 5467, 5852, 5909, 5917
PvuII CAGCTG 5 cut(s) 556, 4021, 5308, 5575, 6539
RruI TCGCGA 1 cut(s) 5001
SacI GAGCTC 2 cut(s) 606, 5031
SapI GCTCTTC 2 cut(s) 3174, 5052
Sau96I GGNCC 9 cut(s) 505, 2065, 2513, 2702, 3526, 4463, 6084, 6709, 7028
ScaI AGTACT 2 cut(s) 2407, 4381
SexAI ACCWGGT 2 cut(s) 2816, 4279
SfcI CTRYAG 7 cut(s) 1952, 1968, 3858, 4383, 4643, 5300, 5337
Sfr274I CTCGAG 1 cut(s) 2799
SfuI TTCGAA 1 cut(s) 7002
SinI GGWCC 7 cut(s) 2065, 2513, 2702, 3526, 4463, 6709, 7028
SlaI CTCGAG 1 cut(s) 2799
SphI GCATGC 1 cut(s) 4734
SseBI AGGCCT 2 cut(s) 143, 2327
SspI AATATT 7 cut(s) 934, 1572, 1825, 2839, 2935, 3724, 3927
SstI GAGCTC 2 cut(s) 606, 5031
StuI AGGCCT 2 cut(s) 143, 2327
TaiI ACGT 8 cut(s) 875, 3738, 4726, 4968, 6100, 6251, 6394, 6877
TatI WGTACW 7 cut(s) 1291, 2405, 4075, 4236, 4379, 5124, 6807
TauI GCSGC 5 cut(s) 2198, 2645, 4330, 4699, 7026
TseFI GTSAC 4 cut(s) 1773, 1961, 2733, 5809
Tsp45I GTSAC 4 cut(s) 1773, 1961, 2733, 5809
TspGWI ACGGA 4 cut(s) 596, 5340, 5471, 5615
Van91I CCANNNNNTGG 1 cut(s) 5206
Vha464I CTTAAG 2 cut(s) 3247, 5990
VneI GTGCAC 2 cut(s) 2742, 6978
VpaK11BI GGWCC 7 cut(s) 2065, 2513, 2702, 3526, 4463, 6709, 7028
VspI ATTAAT 2 cut(s) 2928, 5361
XagI CCTNNNNNAGG 1 cut(s) 2799
XbaI TCTAGA 1 cut(s) 5899
XceI RCATGY 8 cut(s) 521, 1073, 1157, 1256, 4073, 4237, 4367, 4734
XcmI CCANNNNNNNNNTGG 1 cut(s) 3381
XhoI CTCGAG 1 cut(s) 2799
XmiI GTMKAC 2 cut(s) 1300, 1677
XmnI GAANNNNTTC 2 cut(s) 294, 4687
ZrmI AGTACT 2 cut(s) 2407, 4381
Zsp2I ATGCAT 6 cut(s) 319, 1267, 2281, 4771, 4951, 5971
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.