Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr11

Orthogroup ID

OG0011080

Physical Location & Seq

Genomic Coordinates Forward (+)

24497681 .. 24503710

Gene Structure 6030 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2625 bp

ATGAGCAGAGCCGGACAGGAAGAAGACGAAGACAACGAGTGCTTCTACGAGTCCCTCGATCGCATTGTCTCCTCCTCTTGTTCTTGTTCCACTTCGAACTCCGACTCCGACCCCGACCCCGACCCGAATCCGAAGTATGCCGTACCCAAATTCCCAATGGGCGCCTCCACCAAGTACGACGTTTGGATCTCCGAGCCTTCTTCCGTCTCTGAACGCCGGTCCAAACTCCTCACTGAAATGGGTCTCACCGGCGACCCAGTTCTGACCCGGGCCAAACCCCAACCGGGTTTTTCCGGCGAGTTCGGGCGGTCTGTCTCGTCGGATTATTTGATCACTCAGGTAAATTCCGGCGGTGGAGGCGTTAATAGCATCGTCCGCTCCAAATCTGACGGCGGCGATCAGTGTAATAACGCTTGTTCTACTTCATCTGTTTCTTCTCCCCCAATTCTTTCGATTCGTGGTGCCGGTGAGGCCGAGACCGAGCCTGAACCCGAAACCAGTTCATTTGTAAATAAAATTACAAAATCTTTGGCTTGTAAATCTTCAAGCGGTAAAAGCAACTCATCCCCGCCGCCGAATAAGCCGCCTTCTGGCAAGAATTCGAGGAGGGTAGACGAGATTCGAAGCGATTCGAAGGCGGAGGAGTTGGATTGCAATGACATTGGTAAGGTTGGTGAAAGTGATGCTAATACTAATGCCCAAGTGTGTACTATTAGGAACCTTGATAATGGTAAAGAGTTTGTTGTGAATGAGATTAGGGAAGATGGAATGTGGAACAAGCTTAAGGAAGTGGGTACAGGGAAGCAGTTGACCATGGAGGAGTTTGAGATGAGCGTCGGGCATTCACCGATTGTTCAAGAACTGATGAGGAGGCAGAATGTGGAGGAGGGTCATAAAGATGGTTCCGAGTCCAATGCCAATGGGAGCAATGACGGGGTTTCAAAGTTGAAGAAGAAAGGGGGTTGGTTGAAGAGCATCAAGAGTGTTGCGATCACCATGACAGGTCACAAGGATAGGCGGAGTAGCGATGACAGGGACACATCGTCGGAAAAGGGAGGTCGGAGGTCAAGCTCTGGCACAGATGATAGCCAGGATGTGTCGTTTCATGGGCCGAAGAGGGTGAGGGTGAGGCAGTATGGGAAGTCATGTAAGGAGGTCACTGCATTGTTCAAGAGTCAGGAGATTCAGGCGCATACCGGGTCAATTTGGAGCATTAAATTTAGTTTGGATGGAAAATATTTGGCTAGTGCCGGTGAGGATTGCGTGATTCATATTTGGCAGGTGATGCAGAGTGAGAGGAAGGGTGACCTGTTGATGGAGAAGTCAGAAGATAGCAATTTAAACATGCTGTTATTTGCCAATGGGTCGCCGGAACCATGTTCAGTCACACCAAATGTGGACAACCATGTGGAAAAAAAGAGGAGAGGAAGGTCGTCTATCAGTCGAAAATCTGTTAGTTTGGACCATTATGTGATTCCAGAGACTGTGTTTGCACTTTCAGAGAAACCCTTTTCTTCATTCCAAGGGCATCTGGATGATGTGCTTGACCTTTCTTGGTCCAAGTCGCAGCACCTGCTCTCATCTTCAATGGACAAAACTGTGCGCCTTTGGCACTTGTCGAGCAAGACTTGTCTGAAGATATTTTCACATAGTGACTATGTAACTTGCATCCAGTTCAACCCTGTTGATGATAGGTACTTCATTAGTGGATCACTTGATGCTAAGGTTCGCATATGGAGCATCCCTGATCATCAAGTGGTTGATTGGAATGATCTGCACGAGATGGTGACTGCTGCTTGCTACACTCCTGATGGTCAGGGCGCCCTAGTTGGTTCATACAAAGGCAGCTGCCGTTTATACAATACATCTGAGAACAAGTTGCAACAGAAAAGCGAAATTAATCTGCAGAACAAGAAAAAGAAATCACATCACAAGAAAATTACAGGTTTTCAGTTTGCACCAGGAAGTTCATCAGAAGTACTGATTACATCTGCAGATTCACGAATTAGAGTTGTTGACGGTATTGATCTGGTTCACAAGTACAAGGGATTTCGGAACGCAAATAGCCAAATCTCAGCCACCCTCACGGCAAATGGTAAATATGTGGTATCTGCTAGTGAGGATTCTCATGTATATATATGGAAACATGAAGCTGATTCCCGTCCTAGCAGAAGCAAAAGTGTCACCGTCACGCGCTCATATGAGCATTTCCATTGTCAAGATGTATCAGCAGCCATCCCTTGGCCTGGAATGGCTGATTTATGGGGATTACAAGATTCAGAACGTAATGGGCTTGACGAGGTCTCCCCAGCAAATCACCCGCCTACACCAGTTGAGGCGGCTAATGCCAACGAGGGTTCAAGAGCACCATCTGGTTCCACCAATAGCCCTCTCCACGGAACCATTTCGAGTGCATCCAACAATTACTTCTTTGATAGAATTTCAGCAACATGGCCGGAGGAAAAACTTCTTCTAGCTACTAGGAACCGGAGCCCACGTGTAAGTTTTGATTTTACGAATGGTTTAAGCCAAAACATGTCAGCCTGGGGTGTTGTGATTGTAACTGCTGGGCTTCGAGGTGAAATTAGAACTTTCCAAAATTTTGGGTTGCCTGTTCGGATTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005829 GO:0010008 GO:0012505 GO:0016020 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0048471 GO:0097708 GO:0098588 GO:0098791 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

875

Amino Acids

95.95

Weight (kDa)

6.56

Isoelectric Point (pI)

51.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_THOC3	PF25174	390 - 429	1.5e-07	THOC3 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	392 - 627	3.3e-15	WDR3 second beta-propeller domain
WD40	PF00400	393 - 426	9.4e-08	WD domain, G-beta repeat
Beta-prop_WDR5	PF25175	395 - 434	1.7e-06	WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	482 - 564	9e-08	WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_1st	PF25173	500 - 581	1.4e-12	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	501 - 718	6.5e-37	WDR5 beta-propeller domain
WD40_Prp19	PF24814	501 - 628	7.8e-15	Prp19 WD40 domain
WD40	PF00400	502 - 537	9.1e-07	WD domain, G-beta repeat
EIF3I	PF24805	504 - 703	6.2e-09	EIF3I
WD40_CDC20-Fz	PF24807	504 - 586	3.1e-08	CDC20/Fizzy WD40 domain
Beta-prop_THOC3	PF25174	505 - 547	9.1e-06	THOC3 beta-propeller domain
WDR55	PF24796	505 - 632	7.7e-06	WDR55
Beta-prop_IFT122_1st	PF23381	506 - 623	2e-06	IFT122 first beta-propeller
Beta-prop_CAF1B_HIR1	PF24105	507 - 587	1e-11	CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3	PF25174	511 - 716	1.8e-23	THOC3 beta-propeller domain
Beta-prop_Aladin	PF25460	512 - 631	2.5e-09	Aladin seven-bladed propeller
Beta-prop_WDR90_POC16_2nd	PF23393	522 - 628	1.5e-08	WDR90/POC16, second beta-propeller
WD40_Gbeta	PF25391	523 - 714	5.6e-09	G protein beta WD-40 repeat protein
WD40	PF00400	543 - 580	8.3e-06	WD domain, G-beta repeat
WD40_WDHD1_1st	PF24817	551 - 722	5.7e-12	WDHD1 first WD40 domain
Beta-prop_EML_2	PF23414	565 - 724	1e-12	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st	PF25173	585 - 716	6e-08	WDR3 first beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011080)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G15470 AT5G54200
fragaria_vesca	FvH4_3g20610 FvH4_3g20610
malus_domestica	MD03G1223700.v1.1 MD11G1241600.v1.1
prunus_persica	Prupe.4G185800_v2.0.a1 Prupe.4G185800_v2.0.a1
pyrus_communis	pycom03g17200 pycom03g17210 pycom11g21320
rosa_chinensis	RchiOBHm_Chr5g0034631
rosa_laevigata	RLG00000033560
rosa_roxburghii	Rroxscaffold_1G00045690
rosa_rugosa	Rorug05G0147300
rosa_samantha	Rh5AG239300 Rh5BG240000 Rh5CG270400 Rh5DG248200
rosa_wichuraiana	Rw5G021930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	1270, 1581
Acc36I	ACCTGC	2 cut(s)	1270, 1581
AccB1I	GGYRCC	3 cut(s)	161, 461, 1820
AccB7I	CCANNNNNTGG	1 cut(s)	2241
AccBSI	CCGCTC	1 cut(s)	378
AccI	GTMKAC	1 cut(s)	612
AccII	CGCG	1 cut(s)	2194
AclWI	GGATC	2 cut(s)	194, 1717
AcoI	YGGCCR	1 cut(s)	2453
AcsI	RAATTY	6 cut(s)	149, 343, 598, 1217, 2439, 2599
AcuI	CTGAAG	1 cut(s)	1655
AcvI	CACGTG	1 cut(s)	2498
AcyI	GRCGYC	2 cut(s)	162, 1821
AdeI	CACNNNGTG	1 cut(s)	1652
AfaI	GTAC	7 cut(s)	144, 176, 709, 796, 1697, 1980, 2042
AfiI	CCNNNNNNNGG	6 cut(s)	284, 590, 1315, 2241, 2246, 2396
AflII	CTTAAG	1 cut(s)	782
AflIII	ACRYGT	2 cut(s)	2497, 2535
AgsI	TTSAA	9 cut(s)	546, 857, 942, 949, 970, 1171, 1587, 1678, 2361
AhdI	GACNNNNNGTC	1 cut(s)	1042
AjnI	CCWGG	4 cut(s)	1089, 1960, 2245, 2543
AjuI	GAANNNNNNNTTGG	2 cut(s)	2411, 2443
AloI	GAACNNNNNNTCC	2 cut(s)	89, 121
AluBI	AGCT	5 cut(s)	781, 1071, 1848, 2153, 2477
AluI	AGCT	5 cut(s)	781, 1071, 1848, 2153, 2477
Alw21I	GWGCWC	1 cut(s)	2368
Alw26I	GTCTC	7 cut(s)	73, 211, 248, 319, 470, 1475, 2308
AlwI	GGATC	2 cut(s)	194, 1717
AlwNI	CAGNNNCTG	2 cut(s)	1484, 1573
Ama87I	CYCGRG	1 cut(s)	267
AoxI	GGCC	5 cut(s)	270, 471, 1109, 2243, 2453
ApeKI	GCWGC	5 cut(s)	1567, 1793, 1845, 1848, 2231
ApoI	RAATTY	6 cut(s)	149, 343, 598, 1217, 2439, 2599
AseI	ATTAAT	1 cut(s)	1899
Asp700I	GAANNNNTTC	3 cut(s)	628, 2404, 2466
AspLEI	GCGC	5 cut(s)	164, 1192, 1605, 1823, 2196
AspS9I	GGNCC	5 cut(s)	219, 270, 1109, 1462, 1557
AsuC2I	CCSGG	4 cut(s)	268, 269, 285, 1198
AsuII	TTCGAA	3 cut(s)	95, 622, 632
AvaI	CYCGRG	1 cut(s)	267
AvaII	GGWCC	3 cut(s)	219, 1462, 1557
BanI	GGYRCC	3 cut(s)	161, 461, 1820
BanII	GRGCYC	1 cut(s)	2495
BauI	CACGAG	1 cut(s)	1778
BbrPI	CACGTG	1 cut(s)	2498
BbsI	GAAGAC	2 cut(s)	30, 36
Bbv12I	GWGCWC	1 cut(s)	2368
BbvI	GCAGC	5 cut(s)	1579, 1780, 1835, 1857, 2243
BccI	CCATC	8 cut(s)	758, 893, 1223, 1309, 1777, 1805, 2243, 2377
BceAI	ACGGC	4 cut(s)	125, 406, 1836, 2103
BcgI	CGANNNNNNTGC	2 cut(s)	896, 930
BciT130I	CCWGG	4 cut(s)	1091, 1962, 2247, 2545
BclI	TGATCA	2 cut(s)	330, 1747
BcnI	CCSGG	4 cut(s)	268, 269, 285, 1198
BcoDI	GTCTC	7 cut(s)	73, 211, 248, 319, 470, 1475, 2308
BfaI	CTAG	6 cut(s)	1245, 1826, 2115, 2166, 2474, 2481
BfmI	CTRYAG	2 cut(s)	1904, 1992
BfoI	RGCGCY	2 cut(s)	165, 1824
BfrI	CTTAAG	1 cut(s)	782
BfuAI	ACCTGC	2 cut(s)	1270, 1581
BglI	GCCNNNNNGGC	1 cut(s)	470
BisI	GCNGC	9 cut(s)	394, 572, 584, 1568, 1794, 1846, 1849, 2232, 2340
BlsI	GCNGC	9 cut(s)	395, 573, 585, 1569, 1795, 1847, 1850, 2233, 2341
BmcAI	AGTACT	1 cut(s)	1980
Bme1390I	CCNGG	8 cut(s)	268, 269, 285, 1091, 1198, 1962, 2247, 2545
Bme18I	GGWCC	3 cut(s)	219, 1462, 1557
BmeRI	GACNNNNNGTC	1 cut(s)	1042
BmeT110I	CYCGRG	1 cut(s)	267
BmgT120I	GGNCC	5 cut(s)	219, 270, 1109, 1462, 1557
BmrFI	CCNGG	8 cut(s)	268, 269, 285, 1091, 1198, 1962, 2247, 2545
BmrI	ACTGGG	1 cut(s)	251
BmsI	GCATC	9 cut(s)	378, 673, 984, 1275, 1537, 1677, 1708, 1749, 2423
BmuI	ACTGGG	1 cut(s)	251
BpiI	GAAGAC	2 cut(s)	30, 36
Bpu10I	CCTNAGC	1 cut(s)	1722
Bpu14I	TTCGAA	3 cut(s)	95, 622, 632
BpuMI	CCSGG	4 cut(s)	268, 269, 285, 1198
BsaAI	YACGTR	1 cut(s)	2498
BsaHI	GRCGYC	2 cut(s)	162, 1821
BsaI	GGTCTC	3 cut(s)	248, 470, 2308
BsaJI	CCNNGG	6 cut(s)	267, 813, 1522, 2240, 2395, 2544
BsaWI	WCCGGW	1 cut(s)	2487
BsaXI	ACNNNNNCTCC	6 cut(s)	89, 119, 2288, 2318, 2482, 2512
Bsc4I	CCNNNNNNNGG	6 cut(s)	284, 590, 1315, 2241, 2246, 2396
Bse118I	RCCGGY	4 cut(s)	216, 248, 464, 1250
Bse1I	ACTGG	4 cut(s)	257, 498, 1672, 2330
Bse3DI	GCAATG	2 cut(s)	661, 934
BseBI	CCWGG	4 cut(s)	1091, 1962, 2247, 2545
BseDI	CCNNGG	6 cut(s)	267, 813, 1522, 2240, 2395, 2544
BseGI	GGATG	8 cut(s)	563, 1099, 1234, 1540, 1668, 1740, 2235, 2414
BseLI	CCNNNNNNNGG	6 cut(s)	284, 590, 1315, 2241, 2246, 2396
BseMI	GCAATG	2 cut(s)	661, 934
BseMII	CTCAG	3 cut(s)	350, 1860, 2088
BseNI	ACTGG	4 cut(s)	257, 498, 1672, 2330
BseRI	GAGGAG	9 cut(s)	61, 64, 218, 619, 656, 833, 883, 899, 1435
BseXI	GCAGC	5 cut(s)	1579, 1780, 1835, 1857, 2243
BseYI	CCCAGC	2 cut(s)	2308, 2567
BsgI	GTGCAG	1 cut(s)	1760
Bsh1236I	CGCG	1 cut(s)	2194
Bsh1285I	CGRYCG	1 cut(s)	61
BshFI	GGCC	5 cut(s)	272, 473, 1111, 2245, 2455
BshNI	GGYRCC	3 cut(s)	161, 461, 1820
BsiEI	CGRYCG	1 cut(s)	61
BsiHKAI	GWGCWC	1 cut(s)	2368
BsiHKCI	CYCGRG	1 cut(s)	267
BslFI	GGGAC	2 cut(s)	37, 1049
BslI	CCNNNNNNNGG	6 cut(s)	284, 590, 1315, 2241, 2246, 2396
BsmAI	GTCTC	7 cut(s)	73, 211, 248, 319, 470, 1475, 2308
BsmBI	CGTCTC	1 cut(s)	211
BsmFI	GGGAC	2 cut(s)	37, 1049
BsmI	GAATGC	1 cut(s)	841
BsnI	GGCC	5 cut(s)	272, 473, 1111, 2245, 2455
Bso31I	GGTCTC	3 cut(s)	248, 470, 2308
BsoBI	CYCGRG	1 cut(s)	267
Bsp119I	TTCGAA	3 cut(s)	95, 622, 632
Bsp1286I	GDGCHC	2 cut(s)	2368, 2495
Bsp143I	GATC	9 cut(s)	58, 186, 330, 397, 990, 1709, 1747, 1771, 2026
Bsp19I	CCATGG	1 cut(s)	813
BspANI	GGCC	5 cut(s)	272, 473, 1111, 2245, 2455
BspCNI	CTCAG	3 cut(s)	349, 1861, 2087
BspFNI	CGCG	1 cut(s)	2194
BspMAI	CTGCAG	2 cut(s)	1908, 1996
BspMI	ACCTGC	2 cut(s)	1270, 1581
BspPI	GGATC	2 cut(s)	194, 1717
BspQI	GCTCTTC	1 cut(s)	965
BspT104I	TTCGAA	3 cut(s)	95, 622, 632
BspT107I	GGYRCC	3 cut(s)	161, 461, 1820
BspTI	CTTAAG	1 cut(s)	782
BspTNI	GGTCTC	3 cut(s)	248, 470, 2308
BsrBI	CCGCTC	1 cut(s)	378
BsrDI	GCAATG	2 cut(s)	661, 934
BsrFI	RCCGGY	4 cut(s)	216, 248, 464, 1250
BsrI	ACTGG	4 cut(s)	257, 498, 1672, 2330
BssAI	RCCGGY	4 cut(s)	216, 248, 464, 1250
BssECI	CCNNGG	6 cut(s)	267, 813, 1522, 2240, 2395, 2544
BssMI	GATC	9 cut(s)	58, 186, 330, 397, 990, 1709, 1747, 1771, 2026
BssNI	GRCGYC	2 cut(s)	162, 1821
BssSI	CACGAG	1 cut(s)	1778
BssT1I	CCWWGG	3 cut(s)	813, 1522, 2240
Bst2BI	CACGAG	1 cut(s)	1778
Bst2UI	CCWGG	4 cut(s)	1091, 1962, 2247, 2545
Bst4CI	ACNGT	4 cut(s)	1486, 1600, 2021, 2188
Bst6I	CTCTTC	2 cut(s)	965, 1109
BstACI	GRCGYC	2 cut(s)	162, 1821
BstAFI	CTTAAG	1 cut(s)	782
BstAPI	GCANNNNNTGC	2 cut(s)	1285, 1573
BstBAI	YACGTR	1 cut(s)	2498
BstBI	TTCGAA	3 cut(s)	95, 622, 632
BstC8I	GCNNGC	1 cut(s)	1798
BstDEI	CTNAG	4 cut(s)	336, 1722, 1869, 2074
BstDSI	CCRYGG	2 cut(s)	813, 2395
BstEII	GGTNACC	1 cut(s)	1304
BstF5I	GGATG	8 cut(s)	563, 1099, 1234, 1540, 1668, 1740, 2235, 2414
BstFNI	CGCG	1 cut(s)	2194
BstH2I	RGCGCY	2 cut(s)	165, 1824
BstHHI	GCGC	5 cut(s)	164, 1192, 1605, 1823, 2196
BstKTI	GATC	9 cut(s)	61, 189, 333, 400, 993, 1712, 1750, 1774, 2029
BstMAI	GTCTC	7 cut(s)	73, 211, 248, 319, 470, 1475, 2308
BstMBI	GATC	9 cut(s)	58, 186, 330, 397, 990, 1709, 1747, 1771, 2026
BstMCI	CGRYCG	1 cut(s)	61
BstNI	CCWGG	4 cut(s)	1091, 1962, 2247, 2545
BstNSI	RCATGY	2 cut(s)	1348, 2539
BstPI	GGTNACC	1 cut(s)	1304
BstSCI	CCNGG	8 cut(s)	266, 267, 283, 1089, 1196, 1960, 2245, 2543
BstSFI	CTRYAG	2 cut(s)	1904, 1992
BstUI	CGCG	1 cut(s)	2194
BstV1I	GCAGC	5 cut(s)	1579, 1780, 1835, 1857, 2243
BstV2I	GAAGAC	2 cut(s)	30, 36
BstX2I	RGATCY	1 cut(s)	186
BstXI	CCANNNNNNTGG	1 cut(s)	2603
BstYI	RGATCY	1 cut(s)	186
BsuRI	GGCC	5 cut(s)	272, 473, 1111, 2245, 2455
BtgI	CCRYGG	2 cut(s)	813, 2395
BtgZI	GCGATG	1 cut(s)	1041
BtsCI	GGATG	8 cut(s)	563, 1099, 1234, 1540, 1668, 1740, 2235, 2414
BtsI	GCAGTG	1 cut(s)	1158
BtsIMutI	CAGTG	3 cut(s)	231, 407, 1158
BveI	ACCTGC	2 cut(s)	1270, 1581
Cac8I	GCNNGC	1 cut(s)	1798
CaiI	CAGNNNCTG	2 cut(s)	1484, 1573
CfoI	GCGC	5 cut(s)	164, 1192, 1605, 1823, 2196
Cfr10I	RCCGGY	4 cut(s)	216, 248, 464, 1250
Cfr13I	GGNCC	5 cut(s)	219, 270, 1109, 1462, 1557
Cfr9I	CCCGGG	1 cut(s)	267
CseI	GACGC	1 cut(s)	823
Csp6I	GTAC	7 cut(s)	143, 175, 708, 795, 1696, 1979, 2041
CviQI	GTAC	7 cut(s)	143, 175, 708, 795, 1696, 1979, 2041
DdeI	CTNAG	4 cut(s)	336, 1722, 1869, 2074
DinI	GGCGCC	2 cut(s)	163, 1822
DpnI	GATC	9 cut(s)	60, 188, 332, 399, 992, 1711, 1749, 1773, 2028
DpnII	GATC	9 cut(s)	58, 186, 330, 397, 990, 1709, 1747, 1771, 2026
DraI	TTTAAA	1 cut(s)	1341
DraIII	CACNNNGTG	1 cut(s)	1652
DriI	GACNNNNNGTC	1 cut(s)	1042
EaeI	YGGCCR	1 cut(s)	2453
Eam1104I	CTCTTC	2 cut(s)	965, 1109
Eam1105I	GACNNNNNGTC	1 cut(s)	1042
EarI	CTCTTC	2 cut(s)	965, 1109
EciI	GGCGGA	2 cut(s)	653, 1033
Eco130I	CCWWGG	3 cut(s)	813, 1522, 2240
Eco24I	GRGCYC	1 cut(s)	2495
Eco31I	GGTCTC	3 cut(s)	248, 470, 2308
Eco47I	GGWCC	3 cut(s)	219, 1462, 1557
Eco57I	CTGAAG	1 cut(s)	1655
Eco72I	CACGTG	1 cut(s)	2498
Eco88I	CYCGRG	1 cut(s)	267
Eco91I	GGTNACC	1 cut(s)	1304
EcoO65I	GGTNACC	1 cut(s)	1304
EcoRI	GAATTC	1 cut(s)	598
EcoRII	CCWGG	4 cut(s)	1089, 1960, 2245, 2543
EcoT14I	CCWWGG	3 cut(s)	813, 1522, 2240
EcoT38I	GRGCYC	1 cut(s)	2495
EgeI	GGCGCC	2 cut(s)	163, 1822
EheI	GGCGCC	2 cut(s)	163, 1822
ErhI	CCWWGG	3 cut(s)	813, 1522, 2240
Esp3I	CGTCTC	1 cut(s)	211
FaqI	GGGAC	2 cut(s)	37, 1049
FauI	CCCGC	2 cut(s)	576, 2328
FauNDI	CATATG	2 cut(s)	1733, 2200
FbaI	TGATCA	2 cut(s)	330, 1747
FblI	GTMKAC	1 cut(s)	612
Fnu4HI	GCNGC	9 cut(s)	394, 572, 584, 1568, 1794, 1846, 1849, 2232, 2340
FokI	GGATG	8 cut(s)	550, 1106, 1241, 1547, 1655, 1727, 2222, 2401
FriOI	GRGCYC	1 cut(s)	2495
Fsp4HI	GCNGC	9 cut(s)	394, 572, 584, 1568, 1794, 1846, 1849, 2232, 2340
FspBI	CTAG	6 cut(s)	1245, 1826, 2115, 2166, 2474, 2481
GlaI	GCGC	5 cut(s)	163, 1191, 1604, 1822, 2195
GluI	GCNGC	9 cut(s)	394, 572, 584, 1568, 1794, 1846, 1849, 2232, 2340
GsaI	CCCAGC	2 cut(s)	2312, 2571
HaeII	RGCGCY	2 cut(s)	165, 1824
HaeIII	GGCC	5 cut(s)	272, 473, 1111, 2245, 2455
HgaI	GACGC	1 cut(s)	823
HhaI	GCGC	5 cut(s)	164, 1192, 1605, 1823, 2196
Hin1I	GRCGYC	2 cut(s)	162, 1821
Hin6I	GCGC	5 cut(s)	162, 1190, 1603, 1821, 2194
HinP1I	GCGC	5 cut(s)	162, 1190, 1603, 1821, 2194
HincII	GTYRAC	2 cut(s)	810, 2017
HindII	GTYRAC	2 cut(s)	810, 2017
HindIII	AAGCTT	1 cut(s)	779
Hpy166II	GTNNAC	6 cut(s)	613, 708, 810, 1399, 2017, 2035
Hpy8I	GTNNAC	6 cut(s)	613, 708, 810, 1399, 2017, 2035
Hpy99I	CGWCG	4 cut(s)	182, 322, 839, 1048
HpyAV	CCTTC	5 cut(s)	207, 597, 628, 1294, 1422
HpyCH4III	ACNGT	4 cut(s)	1486, 1600, 2021, 2188
HpyCH4IV	ACGT	3 cut(s)	180, 2284, 2497
HpyF3I	CTNAG	4 cut(s)	336, 1722, 1869, 2074
HpySE526I	ACGT	3 cut(s)	180, 2284, 2497
Hsp92I	GRCGYC	2 cut(s)	162, 1821
HspAI	GCGC	5 cut(s)	162, 1190, 1603, 1821, 2194
KasI	GGCGCC	2 cut(s)	161, 1820
Ksp22I	TGATCA	2 cut(s)	330, 1747
Kzo9I	GATC	9 cut(s)	58, 186, 330, 397, 990, 1709, 1747, 1771, 2026
LguI	GCTCTTC	1 cut(s)	965
LmnI	GCTCC	5 cut(s)	383, 924, 1209, 1737, 2490
Lsp1109I	GCAGC	5 cut(s)	1579, 1780, 1835, 1857, 2243
LweI	GCATC	9 cut(s)	378, 673, 984, 1275, 1537, 1677, 1708, 1749, 2423
MaeI	CTAG	6 cut(s)	1245, 1826, 2115, 2166, 2474, 2481
MaeII	ACGT	3 cut(s)	180, 2284, 2497
MalI	GATC	9 cut(s)	60, 188, 332, 399, 992, 1711, 1749, 1773, 2028
MbiI	CCGCTC	1 cut(s)	378
MboI	GATC	9 cut(s)	58, 186, 330, 397, 990, 1709, 1747, 1771, 2026
MflI	RGATCY	1 cut(s)	186
MhlI	GDGCHC	2 cut(s)	2368, 2495
Mly113I	GGCGCC	2 cut(s)	162, 1821
MlyI	GAGTC	4 cut(s)	59, 98, 917, 1183
MmeI	TCCRAC	7 cut(s)	126, 132, 300, 627, 1026, 1040, 2442
MroXI	GAANNNNTTC	3 cut(s)	628, 2404, 2466
MseI	TTAA	6 cut(s)	363, 783, 1215, 1340, 1899, 2525
MslI	CAYNNNNRTG	3 cut(s)	236, 897, 1533
MspA1I	CMGCKG	1 cut(s)	1848
MspCI	CTTAAG	1 cut(s)	782
MspR9I	CCNGG	8 cut(s)	268, 269, 285, 1091, 1198, 1962, 2247, 2545
Mva1269I	GAATGC	1 cut(s)	841
MvaI	CCWGG	4 cut(s)	1091, 1962, 2247, 2545
MvnI	CGCG	1 cut(s)	2194
NarI	GGCGCC	2 cut(s)	162, 1821
NciI	CCSGG	4 cut(s)	268, 269, 285, 1198
NcoI	CCATGG	1 cut(s)	813
NdeI	CATATG	2 cut(s)	1733, 2200
NdeII	GATC	9 cut(s)	58, 186, 330, 397, 990, 1709, 1747, 1771, 2026
NmeAIII	GCCGAG	1 cut(s)	499
NmuCI	GTSAC	8 cut(s)	1004, 1156, 1304, 1384, 1652, 1786, 2182, 2188
NspI	RCATGY	2 cut(s)	1348, 2539
NspV	TTCGAA	3 cut(s)	95, 622, 632
PaqCI	CACCTGC	2 cut(s)	1270, 1581
PciI	ACATGT	1 cut(s)	2535
PciSI	GCTCTTC	1 cut(s)	965
PcsI	WCGNNNNNNNCGW	2 cut(s)	33, 54
PctI	GAATGC	1 cut(s)	841
PdmI	GAANNNNTTC	3 cut(s)	628, 2404, 2466
PflFI	GACNNNGTC	1 cut(s)	2300
PflMI	CCANNNNNTGG	1 cut(s)	2241
PkrI	GCNGC	9 cut(s)	395, 573, 585, 1569, 1795, 1847, 1850, 2233, 2341
Ple19I	CGATCG	1 cut(s)	61
PleI	GAGTC	4 cut(s)	58, 98, 916, 1182
PluTI	GGCGCC	2 cut(s)	165, 1824
PmaCI	CACGTG	1 cut(s)	2498
PmlI	CACGTG	1 cut(s)	2498
PpsI	GAGTC	4 cut(s)	58, 98, 916, 1182
Ppu21I	YACGTR	1 cut(s)	2498
PscI	ACATGT	1 cut(s)	2535
PshBI	ATTAAT	1 cut(s)	1899
Psp6I	CCWGG	4 cut(s)	1089, 1960, 2245, 2543
PspCI	CACGTG	1 cut(s)	2498
PspEI	GGTNACC	1 cut(s)	1304
PspFI	CCCAGC	2 cut(s)	2308, 2567
PspGI	CCWGG	4 cut(s)	1089, 1960, 2245, 2543
PspPI	GGNCC	5 cut(s)	219, 270, 1109, 1462, 1557
PstI	CTGCAG	2 cut(s)	1908, 1996
PstNI	CAGNNNCTG	2 cut(s)	1484, 1573
PsuI	RGATCY	1 cut(s)	186
PsyI	GACNNNGTC	1 cut(s)	2300
PvuI	CGATCG	1 cut(s)	61
PvuII	CAGCTG	1 cut(s)	1848
RsaI	GTAC	7 cut(s)	144, 176, 709, 796, 1697, 1980, 2042
RsaNI	GTAC	7 cut(s)	143, 175, 708, 795, 1696, 1979, 2041
RseI	CAYNNNNRTG	3 cut(s)	236, 897, 1533
SapI	GCTCTTC	1 cut(s)	965
SaqAI	TTAA	6 cut(s)	363, 783, 1215, 1340, 1899, 2525
SatI	GCNGC	9 cut(s)	394, 572, 584, 1568, 1794, 1846, 1849, 2232, 2340
Sau3AI	GATC	9 cut(s)	58, 186, 330, 397, 990, 1709, 1747, 1771, 2026
Sau96I	GGNCC	5 cut(s)	219, 270, 1109, 1462, 1557
ScaI	AGTACT	1 cut(s)	1980
SchI	GAGTC	4 cut(s)	59, 98, 917, 1183
ScrFI	CCNGG	8 cut(s)	268, 269, 285, 1091, 1198, 1962, 2247, 2545
SduI	GDGCHC	2 cut(s)	2368, 2495
SfaNI	GCATC	9 cut(s)	378, 673, 984, 1275, 1537, 1677, 1708, 1749, 2423
SfcI	CTRYAG	2 cut(s)	1904, 1992
SfoI	GGCGCC	2 cut(s)	163, 1822
SfuI	TTCGAA	3 cut(s)	95, 622, 632
SgrAI	CRCCGGYG	1 cut(s)	248
SinI	GGWCC	3 cut(s)	219, 1462, 1557
SmaI	CCCGGG	1 cut(s)	269
SmiMI	CAYNNNNRTG	3 cut(s)	236, 897, 1533
SmlI	CTYRAG	1 cut(s)	782
SmoI	CTYRAG	1 cut(s)	782
SspDI	GGCGCC	2 cut(s)	161, 1820
SspI	AATATT	1 cut(s)	1238
SspMI	CTAG	6 cut(s)	1245, 1826, 2115, 2166, 2474, 2481
StyD4I	CCNGG	8 cut(s)	266, 267, 283, 1089, 1196, 1960, 2245, 2543
StyI	CCWWGG	3 cut(s)	813, 1522, 2240
TaaI	ACNGT	4 cut(s)	1486, 1600, 2021, 2188
TaiI	ACGT	3 cut(s)	183, 2287, 2500
TaqII	GACCGA	1 cut(s)	494
TatI	WGTACW	3 cut(s)	707, 1978, 2040
TauI	GCSGC	4 cut(s)	396, 574, 586, 2342
Tru1I	TTAA	6 cut(s)	363, 783, 1215, 1340, 1899, 2525
Tru9I	TTAA	6 cut(s)	363, 783, 1215, 1340, 1899, 2525
TscAI	CASTG	3 cut(s)	238, 407, 1165
TseFI	GTSAC	8 cut(s)	1004, 1156, 1304, 1384, 1652, 1786, 2182, 2188
TseI	GCWGC	5 cut(s)	1567, 1793, 1845, 1848, 2231
Tsp45I	GTSAC	8 cut(s)	1004, 1156, 1304, 1384, 1652, 1786, 2182, 2188
TspDTI	ATGAA	9 cut(s)	414, 492, 1094, 1259, 1506, 1690, 1824, 1959, 2163
TspGWI	ACGGA	2 cut(s)	193, 2412
TspMI	CCCGGG	1 cut(s)	267
TspRI	CASTG	3 cut(s)	238, 407, 1165
Tth111I	GACNNNGTC	1 cut(s)	2300
Van91I	CCANNNNNTGG	1 cut(s)	2241
Vha464I	CTTAAG	1 cut(s)	782
VpaK11BI	GGWCC	3 cut(s)	219, 1462, 1557
VspI	ATTAAT	1 cut(s)	1899
XapI	RAATTY	6 cut(s)	149, 343, 598, 1217, 2439, 2599
XceI	RCATGY	2 cut(s)	1348, 2539
XcmI	CCANNNNNNNNNTGG	1 cut(s)	154
XmaI	CCCGGG	1 cut(s)	267
XmiI	GTMKAC	1 cut(s)	612
XmnI	GAANNNNTTC	3 cut(s)	628, 2404, 2466
XspI	CTAG	6 cut(s)	1245, 1826, 2115, 2166, 2474, 2481
ZrmI	AGTACT	1 cut(s)	1980

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.