pycom12397g00110

Involved in protein precursor import into chloroplasts. May be part of an intermediate translocation complex acting as a protein-conducting channel at the inner envelope

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00012397
OG0011870
Physical Location & Seq
Forward (+)
50347 .. 64609
14263 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 642 bp
ATGATTTTAAAATCTTTTATACTAGGTAATCTAGTATCCTTATGCATGAAGATAATCAATTCGGTCGTTGTGGTCGGACTCTATTATGGATTTCTGACCACATTCTCCATAGGGCCCTCTTATCTCTTCCTTCTCCGAGCTCGGGTTATGGAAGAAGGAGAAGAAGGAACCGAGAAGAAAGTATCAGCAACAACCGGTTTTATTACGGGACAGCTCATGATGTTCATATCGATCTATTATGTGCCTCTGCATCTAGCATTGGGTAGACCTCATACAATAACTGTCCTAGCTTTACCGTATCTTTTGTTTCATTTCTTCTGGAACAATCACAAACACTTTTTTGATTATGGATCTACTACCAGAAATTCAATGCGTAATCTTAGCATTCAATGGTTATTCCTGAATAATCTCATTTTTCAATTATTCAACCATTTCATTTTACCAAGTTCAATGTTAGTCAGATTAGTCAACATTTATATGTTTCGATGCAACAACAAGATGTTATTTGTAACAAGTAGTTTTGTTGGTTGGTTAATTGGTCACATTTTATTCATGAAATGGGTTGGGTTGGTATTAGGCTGGATACAGCAAAATAATTCTATTAGATCTAATGTACTTATTAGATCTAATAAGTACCTTTAG

Protein Analysis

214

Amino Acids

24.76

Weight (kDa)

9.96

Isoelectric Point (pI)

41.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ycf1 PF05758 21 - 213 1e-133 Ycf1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 265
AclWI GGATC 1 cut(s) 358
AcsI RAATTY 1 cut(s) 364
AfaI GTAC 2 cut(s) 615, 635
AfiI CCNNNNNNNGG 1 cut(s) 142
AgeI ACCGGT 1 cut(s) 194
AgsI TTSAA 5 cut(s) 369, 389, 419, 427, 450
AluBI AGCT 3 cut(s) 140, 214, 290
AluI AGCT 3 cut(s) 140, 214, 290
Alw21I GWGCWC 1 cut(s) 142
AlwI GGATC 1 cut(s) 358
Ama87I CYCGRG 1 cut(s) 141
AoxI GGCC 1 cut(s) 113
ApaI GGGCCC 1 cut(s) 117
ApoI RAATTY 1 cut(s) 364
AsiGI ACCGGT 1 cut(s) 194
AspS9I GGNCC 2 cut(s) 113, 114
AvaI CYCGRG 1 cut(s) 141
BaeGI GKGCMC 1 cut(s) 117
BanII GRGCYC 2 cut(s) 117, 142
Bbv12I GWGCWC 1 cut(s) 142
BciVI GTATCC 2 cut(s) 46, 576
BfaI CTAG 4 cut(s) 23, 32, 254, 287
BfuI GTATCC 2 cut(s) 46, 576
BglII AGATCT 2 cut(s) 605, 623
BmeT110I CYCGRG 1 cut(s) 141
BmgT120I GGNCC 2 cut(s) 113, 114
BmiI GGNNCC 2 cut(s) 115, 169
BmsI GCATC 2 cut(s) 259, 476
Bsa29I ATCGAT 1 cut(s) 230
BsaWI WCCGGW 1 cut(s) 194
Bsc4I CCNNNNNNNGG 1 cut(s) 142
Bse118I RCCGGY 1 cut(s) 194
BseCI ATCGAT 1 cut(s) 230
BseLI CCNNNNNNNGG 1 cut(s) 142
BseSI GKGCMC 1 cut(s) 117
Bsh1285I CGRYCG 1 cut(s) 66
BshFI GGCC 1 cut(s) 115
BshTI ACCGGT 1 cut(s) 194
BshVI ATCGAT 1 cut(s) 230
BsiEI CGRYCG 1 cut(s) 66
BsiHKAI GWGCWC 1 cut(s) 142
BsiHKCI CYCGRG 1 cut(s) 141
BsiSI CCGG 1 cut(s) 195
BslFI GGGAC 1 cut(s) 222
BslI CCNNNNNNNGG 1 cut(s) 142
BsmFI GGGAC 1 cut(s) 222
BsmI GAATGC 1 cut(s) 384
BsnI GGCC 1 cut(s) 115
BsoBI CYCGRG 1 cut(s) 141
Bsp120I GGGCCC 1 cut(s) 113
Bsp1286I GDGCHC 2 cut(s) 117, 142
Bsp143I GATC 4 cut(s) 231, 350, 605, 623
BspANI GGCC 1 cut(s) 115
BspDI ATCGAT 1 cut(s) 230
BspHI TCATGA 2 cut(s) 216, 552
BspLI GGNNCC 2 cut(s) 115, 169
BspPI GGATC 1 cut(s) 358
BsrFI RCCGGY 1 cut(s) 194
BssAI RCCGGY 1 cut(s) 194
BssMI GATC 4 cut(s) 231, 350, 605, 623
Bst4CI ACNGT 2 cut(s) 283, 297
Bst6I CTCTTC 1 cut(s) 131
BstDEI CTNAG 1 cut(s) 380
BstKTI GATC 4 cut(s) 234, 353, 608, 626
BstMBI GATC 4 cut(s) 231, 350, 605, 623
BstMCI CGRYCG 1 cut(s) 66
BstSLI GKGCMC 1 cut(s) 117
BstX2I RGATCY 3 cut(s) 350, 605, 623
BstYI RGATCY 3 cut(s) 350, 605, 623
Bsu15I ATCGAT 1 cut(s) 230
BsuI GTATCC 2 cut(s) 46, 576
BsuRI GGCC 1 cut(s) 115
BsuTUI ATCGAT 1 cut(s) 230
CciI TCATGA 2 cut(s) 216, 552
Cfr10I RCCGGY 1 cut(s) 194
Cfr13I GGNCC 2 cut(s) 113, 114
ClaI ATCGAT 1 cut(s) 230
Csp6I GTAC 2 cut(s) 614, 634
CspAI ACCGGT 1 cut(s) 194
CviAII CATG 3 cut(s) 46, 217, 553
CviJI RGCY 5 cut(s) 115, 140, 214, 290, 579
CviKI_1 RGCY 5 cut(s) 115, 140, 214, 290, 579
CviQI GTAC 2 cut(s) 614, 634
DdeI CTNAG 1 cut(s) 380
DpnI GATC 4 cut(s) 233, 352, 607, 625
DpnII GATC 4 cut(s) 231, 350, 605, 623
DraI TTTAAA 1 cut(s) 9
Eam1104I CTCTTC 1 cut(s) 131
EarI CTCTTC 1 cut(s) 131
Ecl136II GAGCTC 1 cut(s) 140
Eco24I GRGCYC 2 cut(s) 117, 142
Eco53kI GAGCTC 1 cut(s) 140
Eco88I CYCGRG 1 cut(s) 141
EcoICRI GAGCTC 1 cut(s) 140
EcoO109I RGGNCCY 2 cut(s) 113, 114
EcoT22I ATGCAT 1 cut(s) 47
EcoT38I GRGCYC 2 cut(s) 117, 142
FaeI CATG 3 cut(s) 49, 220, 556
FaqI GGGAC 1 cut(s) 222
FatI CATG 3 cut(s) 45, 216, 552
FblI GTMKAC 1 cut(s) 265
FriOI GRGCYC 2 cut(s) 117, 142
FspBI CTAG 4 cut(s) 23, 32, 254, 287
HaeIII GGCC 1 cut(s) 115
HapII CCGG 1 cut(s) 195
Hin1II CATG 3 cut(s) 49, 220, 556
HincII GTYRAC 1 cut(s) 469
HindII GTYRAC 1 cut(s) 469
HinfI GANTC 1 cut(s) 78
HpaII CCGG 1 cut(s) 195
Hpy166II GTNNAC 2 cut(s) 266, 469
Hpy188I TCNGA 4 cut(s) 77, 96, 137, 461
Hpy188III TCNNGA 4 cut(s) 217, 319, 400, 553
Hpy8I GTNNAC 2 cut(s) 266, 469
HpyAV CCTTC 3 cut(s) 140, 149, 158
HpyCH4III ACNGT 2 cut(s) 283, 297
HpyCH4V TGCA 3 cut(s) 45, 250, 489
HpyF3I CTNAG 1 cut(s) 380
Hsp92II CATG 3 cut(s) 49, 220, 556
Kzo9I GATC 4 cut(s) 231, 350, 605, 623
LpnPI CCDG 5 cut(s) 208, 304, 373, 413, 565
LweI GCATC 2 cut(s) 259, 476
MaeI CTAG 4 cut(s) 23, 32, 254, 287
MaeIII GTNAC 2 cut(s) 508, 539
MalI GATC 4 cut(s) 233, 352, 607, 625
MboI GATC 4 cut(s) 231, 350, 605, 623
MboII GAAGA 6 cut(s) 61, 118, 164, 173, 187, 307
MflI RGATCY 3 cut(s) 350, 605, 623
MhlI GDGCHC 2 cut(s) 117, 142
MluCI AATT 5 cut(s) 58, 364, 419, 534, 595
MlyI GAGTC 1 cut(s) 72
MmeI TCCRAC 1 cut(s) 55
MnlI CCTC 3 cut(s) 127, 255, 279
Mph1103I ATGCAT 1 cut(s) 47
MseI TTAA 2 cut(s) 8, 533
MslI CAYNNNNRTG 1 cut(s) 476
MspI CCGG 1 cut(s) 195
Mva1269I GAATGC 1 cut(s) 384
NdeII GATC 4 cut(s) 231, 350, 605, 623
NlaIII CATG 3 cut(s) 49, 220, 556
NlaIV GGNNCC 2 cut(s) 115, 169
NmuCI GTSAC 1 cut(s) 539
NsiI ATGCAT 1 cut(s) 47
PagI TCATGA 2 cut(s) 216, 552
PctI GAATGC 1 cut(s) 384
PinAI ACCGGT 1 cut(s) 194
PleI GAGTC 1 cut(s) 72
PpsI GAGTC 1 cut(s) 72
Psp124BI GAGCTC 1 cut(s) 142
PspN4I GGNNCC 2 cut(s) 115, 169
PspOMI GGGCCC 1 cut(s) 113
PspPI GGNCC 2 cut(s) 113, 114
PsuI RGATCY 3 cut(s) 350, 605, 623
RsaI GTAC 2 cut(s) 615, 635
RsaNI GTAC 2 cut(s) 614, 634
RseI CAYNNNNRTG 1 cut(s) 476
SacI GAGCTC 1 cut(s) 142
SaqAI TTAA 2 cut(s) 8, 533
Sau3AI GATC 4 cut(s) 231, 350, 605, 623
Sau96I GGNCC 2 cut(s) 113, 114
SchI GAGTC 1 cut(s) 72
SduI GDGCHC 2 cut(s) 117, 142
SetI ASST 6 cut(s) 28, 142, 216, 271, 292, 639
SfaNI GCATC 2 cut(s) 259, 476
SmiMI CAYNNNNRTG 1 cut(s) 476
Sse9I AATT 5 cut(s) 58, 364, 419, 534, 595
SspMI CTAG 4 cut(s) 23, 32, 254, 287
SstI GAGCTC 1 cut(s) 142
TaaI ACNGT 2 cut(s) 283, 297
TaqI TCGA 2 cut(s) 230, 484
TaqII GACCGA 1 cut(s) 52
TasI AATT 5 cut(s) 58, 364, 419, 534, 595
TatI WGTACW 1 cut(s) 613
Tru1I TTAA 2 cut(s) 8, 533
Tru9I TTAA 2 cut(s) 8, 533
TseFI GTSAC 1 cut(s) 539
Tsp45I GTSAC 1 cut(s) 539
TspDTI ATGAA 6 cut(s) 62, 214, 299, 424, 541, 569
XapI RAATTY 1 cut(s) 364
XmiI GTMKAC 1 cut(s) 265
XspI CTAG 4 cut(s) 23, 32, 254, 287
Zsp2I ATGCAT 1 cut(s) 47
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.