pycom12g10940

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0011045
Physical Location & Seq
Forward (+)
13045427 .. 13050715
5289 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1746 bp
ATGGCAAACCCTATCCTATCCTTCATTGATCTTGTTGACGGAGGACTCAGCGAGGAATCCGATGAAGACGAATCTGAAGAAGAAGAAGACGAGGAAGAAGAAGGCACAGTGGACGCTGATTCTGCTGTGTACCATGCAGATCTAACCGGTGCAGCCGGAAGTGCTCCTGAAACCGCGGCGGCGAGGAATGGGGATGAGGAAGTTGGAAATAAGCGTAGAAGGAGAGGAGGAAGCGAAGCGGTGTCGGTGGATGGAGTTGGCAGTGCAGAGTGCTCCCAAGGCAGCAGCAGCCAATGGAATCGGAACGAAATGGACGGGCTATTTTGCTCTATCTGCTTGGAGCCTTGGACCAATGACGGTGATCATCATATCAGTTGTCTTCCTTGTGGACACCTGTTCGGCATGTCTTGCATAAAACGATGGCTACAGCAGCGTAGCAATTCGGGGAAGTGTCCTCAATGCAACAAAAAATGTGCGTTGAAGGATGTTAGGAAACTTTTTGTATCCCAAATTGTTTCTGTTGATGAAGAGTCACAAAAGAGGGCAGCTTATTTGGAGCATCTATTAGGAGACACACAGAACGGAACATCTGGAGTAGTCAATGCTAATGGGGTCCGTGAAGGAAGATTTGTGGCTGGGCGAAACTTTGGATCAAAGTTTTGTGGACAAGCATATTCTTGCAATTTCATATTAGAGAAGGAGTTGCAGGTAGATGGTGCTCGTTTGTTTGATGTTGATGAATCTAACCAAATTATTTTGATTTCACGAAGGCTCGATGGGATAGGGGGAAGACATGTGCTTACCAAGATGAGTTTGATACCTTCGTATGACAGAGATGATATTCTCCTTCCTAGAGATACAAGAGCCATTAGGGATCTACGCATTTCTTCTTCTAACAGTGATCTTGCACTCTTTGCTTGCTTGGGGAAGAAATTGTCAGTTCTCAGTATGGAAAGCAACAATGTCATCCTTACCTATGATCTACCGGCTGCTGCTTGGACATGTTCATGGGATCTCAACAATCCATATTATGCCTATGCTGGTTTACAGAATGGCTCTCTTTGGGTGTTTGACATGCGCCAAACTGCAGGGCCTGTTGAATCTCGGAGAGGGCTTTCAAACAACCCAATTCATACTGTACATTCCCTTTCACATAATTCAGCTCTTCCTTCTGGTGCTAGATCGGTGCTCTCCGCTTCTTCGATTGGCTTATGTCTGTGGAACTTTGGTTGTGCCGAAGAAGGGCCGACATTAGTCCCTCAAACCGAGAATCAAGGAGTTTGCATATCACTGGCGTATTGTCCTAGCAATGATGACATTGTTGCTTCCTACCGTCCCAAAGTTGAAATGCCTGATGAGACGCTCTTGTCTCAACCGCAGCTTACTCCTACCCGTGGTGGCTTTGGACAAGGAACAGTGGGTTCCCATGTTCTCTTGAAGAGAGCAGACATCAACTGTGTTGATAGGTTGGGTTCTGCATGCACCAATGTATGTGATATCGGGCTTCCAAAATCTGCAATCATCGACGTTGGAAATCATAGCACATTGTTTGCCTCTCAGGATAAGTTGAGTTCCGAATTAGTCTTACAAGAGCTACCGTCTTTTATGACCTTACAGCGCCTTAAATTACAGAAAGATCCTCTTCGCGATCTGAAGTACAGTCCGGCGATAAAACAAGGTCTACTTAGTTGTTTAAGTGGTGAGAAACTGCAACTCTTCAGTACCAAGCTTTCAGGAAAGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000077 GO:0000278 GO:0000724 GO:0000725 GO:0002039 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006302 GO:0006310 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0007093 GO:0007346 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009628 GO:0009987 GO:0010212 GO:0010564 GO:0010948 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0022402 GO:0031297 GO:0031570 GO:0031571 GO:0031974 GO:0031981 GO:0032446 GO:0033554 GO:0034641 GO:0034645 GO:0036211 GO:0036297 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0044773 GO:0044774 GO:0044783 GO:0044819 GO:0045005 GO:0045786 GO:0045930 GO:0046483 GO:0048519 GO:0048523 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0051726 GO:0065007 GO:0070013 GO:0070647 GO:0071704 GO:0080134 GO:0080135 GO:0090304 GO:0090734 GO:0097371 GO:0140096 GO:1901360 GO:1901564 GO:1901576 GO:1901976 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902806 GO:1902807 GO:1903047 GO:2000001 GO:2000045 GO:2000134 GO:2001020
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

582

Amino Acids

63.16

Weight (kDa)

5.17

Isoelectric Point (pI)

56.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 109 - 155 2.9e-09 Ring finger domain
zf-C3HC4 PF00097 109 - 154 3.3e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 109 - 152 2.6e-06 RING-type zinc-finger
WD40_RFWD3 PF23419 236 - 573 2.3e-30 E3 ubiquitin-protein ligase RFWD3 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011045)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G03450 AT5G03750
fragaria_vesca FvH4_6g16560 FvH4_6g16560
malus_domestica MD12G1109200.v1.1
prunus_persica Prupe.7G127800_v2.0.a1
pyrus_communis pycom12g10940
rosa_chinensis RchiOBHm_Chr3g0470481
rosa_laevigata RLG00000024263
rosa_multiflora Rmu_sc0002115.1_g000004 Rmu_sc0003381.1_g000011
rosa_roxburghii Rroxscaffold_4G00302270 Rroxscaffold_6G00410770
rosa_rugosa Rorug03G0109400 Rorug03G0109500
rosa_samantha Rh3AG159800 Rh3BG184300 Rh3CG174800 Rh3DG190600
rosa_wichuraiana Rw3G015120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1366
Acc36I ACCTGC 1 cut(s) 697
AccI GTMKAC 1 cut(s) 1681
AccII CGCG 2 cut(s) 176, 1647
AciI CCGC 6 cut(s) 174, 176, 179, 239, 1194, 1376
AclWI GGATC 4 cut(s) 658, 882, 1020, 1631
AcuI CTGAAG 3 cut(s) 96, 1673, 1702
AfaI GTAC 4 cut(s) 131, 1140, 1658, 1723
AfiI CCNNNNNNNGG 2 cut(s) 1242, 1394
AflIII ACRYGT 2 cut(s) 793, 1001
AgeI ACCGGT 1 cut(s) 146
AgsI TTSAA 5 cut(s) 481, 1100, 1119, 1346, 1438
AluBI AGCT 5 cut(s) 548, 1163, 1381, 1594, 1729
AluI AGCT 5 cut(s) 548, 1163, 1381, 1594, 1729
Alw21I GWGCWC 4 cut(s) 166, 275, 721, 1191
Alw26I GTCTC 3 cut(s) 564, 1352, 1374
AlwI GGATC 4 cut(s) 658, 882, 1020, 1631
AlwNI CAGNNNCTG 1 cut(s) 1094
AoxI GGCC 2 cut(s) 1091, 1244
ApeKI GCWGC 9 cut(s) 152, 282, 285, 288, 430, 545, 989, 992, 1378
AsiGI ACCGGT 1 cut(s) 146
AspLEI GCGC 2 cut(s) 1080, 1620
AspS9I GGNCC 4 cut(s) 348, 613, 1091, 1244
AsuHPI GGTGA 2 cut(s) 371, 1712
AvaII GGWCC 2 cut(s) 348, 613
BaeI ACNNNNGTAYC 2 cut(s) 809, 842
BbsI GAAGAC 4 cut(s) 72, 93, 371, 796
Bbv12I GWGCWC 4 cut(s) 166, 275, 721, 1191
BbvI GCAGC 9 cut(s) 164, 294, 297, 300, 442, 557, 976, 979, 1390
BccI CCATC 4 cut(s) 245, 414, 707, 770
BciVI GTATCC 1 cut(s) 514
BclI TGATCA 1 cut(s) 361
BcoDI GTCTC 3 cut(s) 564, 1352, 1374
BfaI CTAG 3 cut(s) 852, 1179, 1305
BfmI CTRYAG 2 cut(s) 425, 1086
BfoI RGCGCY 1 cut(s) 1621
BfuAI ACCTGC 1 cut(s) 697
BfuI GTATCC 1 cut(s) 514
BglII AGATCT 1 cut(s) 139
Bme18I GGWCC 2 cut(s) 348, 613
BmgT120I GGNCC 4 cut(s) 348, 613, 1091, 1244
BmiI GGNNCC 3 cut(s) 342, 614, 1423
BmsI GCATC 1 cut(s) 568
BoxI GACNNNNGTC 1 cut(s) 1253
BpiI GAAGAC 4 cut(s) 72, 93, 371, 796
BpmI CTGGAG 1 cut(s) 612
BsaJI CCNNGG 4 cut(s) 174, 277, 344, 1393
BsaWI WCCGGW 1 cut(s) 146
Bsc4I CCNNNNNNNGG 2 cut(s) 1242, 1394
Bse118I RCCGGY 2 cut(s) 146, 985
Bse1I ACTGG 1 cut(s) 1296
Bse3DI GCAATG 1 cut(s) 1315
BseDI CCNNGG 4 cut(s) 174, 277, 344, 1393
BseGI GGATG 4 cut(s) 199, 256, 490, 966
BseLI CCNNNNNNNGG 2 cut(s) 1242, 1394
BseMI GCAATG 1 cut(s) 1315
BseMII CTCAG 3 cut(s) 61, 958, 1571
BseNI ACTGG 1 cut(s) 1296
BseRI GAGGAG 1 cut(s) 240
BseXI GCAGC 9 cut(s) 164, 294, 297, 300, 442, 557, 976, 979, 1390
BseYI CCCAGC 1 cut(s) 635
BsgI GTGCAG 2 cut(s) 171, 285
Bsh1236I CGCG 2 cut(s) 176, 1647
BshFI GGCC 2 cut(s) 1093, 1246
BshTI ACCGGT 1 cut(s) 146
BsiHKAI GWGCWC 4 cut(s) 166, 275, 721, 1191
BsiSI CCGG 4 cut(s) 147, 156, 986, 1664
BslFI GGGAC 2 cut(s) 1241, 1320
BslI CCNNNNNNNGG 2 cut(s) 1242, 1394
BsmAI GTCTC 3 cut(s) 564, 1352, 1374
BsmBI CGTCTC 1 cut(s) 1352
BsmFI GGGAC 2 cut(s) 1241, 1320
BsnI GGCC 2 cut(s) 1093, 1246
Bsp1286I GDGCHC 4 cut(s) 166, 275, 721, 1191
Bsp1407I TGTACA 1 cut(s) 1138
Bsp68I TCGCGA 1 cut(s) 1647
BspACI CCGC 6 cut(s) 174, 176, 179, 239, 1194, 1376
BspANI GGCC 2 cut(s) 1093, 1246
BspCNI CTCAG 3 cut(s) 60, 957, 1570
BspFNI CGCG 2 cut(s) 176, 1647
BspLI GGNNCC 3 cut(s) 342, 614, 1423
BspMAI CTGCAG 1 cut(s) 1090
BspMI ACCTGC 1 cut(s) 697
BspPI GGATC 4 cut(s) 658, 882, 1020, 1631
BspQI GCTCTTC 1 cut(s) 1170
BsrDI GCAATG 1 cut(s) 1315
BsrFI RCCGGY 2 cut(s) 146, 985
BsrGI TGTACA 1 cut(s) 1138
BsrI ACTGG 1 cut(s) 1296
BssAI RCCGGY 2 cut(s) 146, 985
BssECI CCNNGG 4 cut(s) 174, 277, 344, 1393
BssT1I CCWWGG 2 cut(s) 277, 344
Bst4CI ACNGT 9 cut(s) 109, 359, 899, 1138, 1334, 1417, 1457, 1599, 1661
Bst6I CTCTTC 5 cut(s) 522, 1170, 1433, 1647, 1721
BstAPI GCANNNNNTGC 2 cut(s) 408, 914
BstAUI TGTACA 1 cut(s) 1138
BstC8I GCNNGC 2 cut(s) 919, 1480
BstDEI CTNAG 4 cut(s) 47, 944, 1557, 1685
BstDSI CCRYGG 2 cut(s) 174, 1393
BstF5I GGATG 4 cut(s) 199, 256, 490, 966
BstFNI CGCG 2 cut(s) 176, 1647
BstH2I RGCGCY 1 cut(s) 1621
BstHHI GCGC 2 cut(s) 1080, 1620
BstMAI GTCTC 3 cut(s) 564, 1352, 1374
BstMWI GCNNNNNNNGC 8 cut(s) 122, 161, 279, 288, 333, 408, 430, 914
BstNSI RCATGY 5 cut(s) 406, 797, 1005, 1078, 1482
BstPAI GACNNNNGTC 1 cut(s) 1253
BstSFI CTRYAG 2 cut(s) 425, 1086
BstUI CGCG 2 cut(s) 176, 1647
BstV1I GCAGC 9 cut(s) 164, 294, 297, 300, 442, 557, 976, 979, 1390
BstV2I GAAGAC 4 cut(s) 72, 93, 371, 796
BstX2I RGATCY 4 cut(s) 139, 874, 1012, 1636
BstYI RGATCY 4 cut(s) 139, 874, 1012, 1636
BsuI GTATCC 1 cut(s) 514
BsuRI GGCC 2 cut(s) 1093, 1246
BtgI CCRYGG 2 cut(s) 174, 1393
BtsCI GGATG 4 cut(s) 199, 256, 490, 966
BtsI GCAGTG 1 cut(s) 268
BtsIMutI CAGTG 5 cut(s) 114, 268, 904, 1289, 1422
BtuMI TCGCGA 1 cut(s) 1647
BveI ACCTGC 1 cut(s) 697
Cac8I GCNNGC 2 cut(s) 919, 1480
CaiI CAGNNNCTG 1 cut(s) 1094
CfoI GCGC 2 cut(s) 1080, 1620
Cfr10I RCCGGY 2 cut(s) 146, 985
Cfr13I GGNCC 4 cut(s) 348, 613, 1091, 1244
Cfr42I CCGCGG 1 cut(s) 177
CseI GACGC 2 cut(s) 122, 1369
Csp6I GTAC 4 cut(s) 130, 1139, 1657, 1722
CspAI ACCGGT 1 cut(s) 146
CviAII CATG 8 cut(s) 134, 403, 794, 1002, 1008, 1075, 1427, 1479
CviQI GTAC 4 cut(s) 130, 1139, 1657, 1722
DdeI CTNAG 4 cut(s) 47, 944, 1557, 1685
DrdI GACNNNNNNGTC 1 cut(s) 1366
DseDI GACNNNNNNGTC 1 cut(s) 1366
Eam1104I CTCTTC 5 cut(s) 522, 1170, 1433, 1647, 1721
EarI CTCTTC 5 cut(s) 522, 1170, 1433, 1647, 1721
Eco130I CCWWGG 2 cut(s) 277, 344
Eco32I GATATC 1 cut(s) 1498
Eco47I GGWCC 2 cut(s) 348, 613
Eco57I CTGAAG 3 cut(s) 96, 1673, 1702
EcoO109I RGGNCCY 1 cut(s) 1091
EcoRV GATATC 1 cut(s) 1498
EcoT14I CCWWGG 2 cut(s) 277, 344
ErhI CCWWGG 2 cut(s) 277, 344
Esp3I CGTCTC 1 cut(s) 1352
FaeI CATG 8 cut(s) 137, 406, 797, 1005, 1011, 1078, 1430, 1482
FalI AAGNNNNNCTT 4 cut(s) 1626, 1658, 1668, 1700
FaqI GGGAC 2 cut(s) 1241, 1320
FatI CATG 8 cut(s) 133, 402, 793, 1001, 1007, 1074, 1426, 1478
FbaI TGATCA 1 cut(s) 361
FblI GTMKAC 1 cut(s) 1681
FokI GGATG 4 cut(s) 206, 263, 497, 953
FspBI CTAG 3 cut(s) 852, 1179, 1305
GlaI GCGC 2 cut(s) 1079, 1619
GsaI CCCAGC 1 cut(s) 639
GsuI CTGGAG 1 cut(s) 612
HaeII RGCGCY 1 cut(s) 1621
HaeIII GGCC 2 cut(s) 1093, 1246
HapII CCGG 4 cut(s) 147, 156, 986, 1664
HgaI GACGC 2 cut(s) 122, 1369
HhaI GCGC 2 cut(s) 1080, 1620
Hin1II CATG 8 cut(s) 137, 406, 797, 1005, 1011, 1078, 1430, 1482
Hin6I GCGC 2 cut(s) 1078, 1618
HinP1I GCGC 2 cut(s) 1078, 1618
HincII GTYRAC 1 cut(s) 37
HindII GTYRAC 1 cut(s) 37
HindIII AAGCTT 1 cut(s) 1727
HinfI GANTC 9 cut(s) 45, 56, 71, 119, 298, 530, 740, 1100, 1270
HpaII CCGG 4 cut(s) 147, 156, 986, 1664
HphI GGTGA 2 cut(s) 371, 1712
Hpy166II GTNNAC 7 cut(s) 37, 112, 130, 389, 665, 1046, 1682
Hpy188I TCNGA 6 cut(s) 61, 76, 303, 1107, 1576, 1653
Hpy188III TCNNGA 7 cut(s) 167, 591, 765, 1435, 1559, 1646, 1734
Hpy8I GTNNAC 7 cut(s) 37, 112, 130, 389, 665, 1046, 1682
Hpy99I CGWCG 1 cut(s) 1529
HpyCH4III ACNGT 9 cut(s) 109, 359, 899, 1138, 1334, 1417, 1457, 1599, 1661
HpyCH4IV ACGT 1 cut(s) 1527
HpyF10VI GCNNNNNNNGC 8 cut(s) 122, 161, 279, 288, 333, 408, 430, 914
HpyF3I CTNAG 4 cut(s) 47, 944, 1557, 1685
HpySE526I ACGT 1 cut(s) 1527
Hsp92II CATG 8 cut(s) 137, 406, 797, 1005, 1011, 1078, 1430, 1482
HspAI GCGC 2 cut(s) 1078, 1618
Ksp22I TGATCA 1 cut(s) 361
KspI CCGCGG 1 cut(s) 177
LguI GCTCTTC 1 cut(s) 1170
LmnI GCTCC 4 cut(s) 169, 278, 340, 556
Lsp1109I GCAGC 9 cut(s) 164, 294, 297, 300, 442, 557, 976, 979, 1390
LweI GCATC 1 cut(s) 568
MaeI CTAG 3 cut(s) 852, 1179, 1305
MaeII ACGT 1 cut(s) 1527
MaeIII GTNAC 1 cut(s) 531
MflI RGATCY 4 cut(s) 139, 874, 1012, 1636
MhlI GDGCHC 4 cut(s) 166, 275, 721, 1191
MluCI AATT 9 cut(s) 439, 510, 682, 750, 932, 1128, 1156, 1577, 1625
MlyI GAGTC 2 cut(s) 39, 539
MmeI TCCRAC 2 cut(s) 184, 1510
MseI TTAA 2 cut(s) 1623, 1694
MslI CAYNNNNRTG 1 cut(s) 1006
MspA1I CMGCKG 1 cut(s) 176
MspI CCGG 4 cut(s) 147, 156, 986, 1664
MvnI CGCG 2 cut(s) 176, 1647
MwoI GCNNNNNNNGC 8 cut(s) 122, 161, 279, 288, 333, 408, 430, 914
NlaIII CATG 8 cut(s) 137, 406, 797, 1005, 1011, 1078, 1430, 1482
NlaIV GGNNCC 3 cut(s) 342, 614, 1423
NmuCI GTSAC 1 cut(s) 531
NruI TCGCGA 1 cut(s) 1647
NspI RCATGY 5 cut(s) 406, 797, 1005, 1078, 1482
PaeI GCATGC 1 cut(s) 1482
PciI ACATGT 2 cut(s) 793, 1001
PciSI GCTCTTC 1 cut(s) 1170
PfeI GAWTC 7 cut(s) 56, 71, 119, 298, 740, 1100, 1270
PinAI ACCGGT 1 cut(s) 146
PleI GAGTC 2 cut(s) 39, 538
PpsI GAGTC 2 cut(s) 39, 538
PscI ACATGT 2 cut(s) 793, 1001
PshAI GACNNNNGTC 1 cut(s) 1253
PspFI CCCAGC 1 cut(s) 635
PspN4I GGNNCC 3 cut(s) 342, 614, 1423
PspPI GGNCC 4 cut(s) 348, 613, 1091, 1244
PstI CTGCAG 1 cut(s) 1090
PstNI CAGNNNCTG 1 cut(s) 1094
PsuI RGATCY 4 cut(s) 139, 874, 1012, 1636
RruI TCGCGA 1 cut(s) 1647
RsaI GTAC 4 cut(s) 131, 1140, 1658, 1723
RsaNI GTAC 4 cut(s) 130, 1139, 1657, 1722
RseI CAYNNNNRTG 1 cut(s) 1006
SacII CCGCGG 1 cut(s) 177
SapI GCTCTTC 1 cut(s) 1170
SaqAI TTAA 2 cut(s) 1623, 1694
Sau96I GGNCC 4 cut(s) 348, 613, 1091, 1244
SchI GAGTC 2 cut(s) 39, 539
SduI GDGCHC 4 cut(s) 166, 275, 721, 1191
SfaNI GCATC 1 cut(s) 568
SfcI CTRYAG 2 cut(s) 425, 1086
Sfr303I CCGCGG 1 cut(s) 177
SgrBI CCGCGG 1 cut(s) 177
SinI GGWCC 2 cut(s) 348, 613
SmiMI CAYNNNNRTG 1 cut(s) 1006
SphI GCATGC 1 cut(s) 1482
Sse9I AATT 9 cut(s) 439, 510, 682, 750, 932, 1128, 1156, 1577, 1625
SsiI CCGC 6 cut(s) 174, 176, 179, 239, 1194, 1376
SspMI CTAG 3 cut(s) 852, 1179, 1305
StyI CCWWGG 2 cut(s) 277, 344
TaaI ACNGT 9 cut(s) 109, 359, 899, 1138, 1334, 1417, 1457, 1599, 1661
TaiI ACGT 1 cut(s) 1530
TaqI TCGA 3 cut(s) 774, 1202, 1524
TasI AATT 9 cut(s) 439, 510, 682, 750, 932, 1128, 1156, 1577, 1625
TatI WGTACW 2 cut(s) 1138, 1656
TauI GCSGC 2 cut(s) 179, 182
TfiI GAWTC 7 cut(s) 56, 71, 119, 298, 740, 1100, 1270
Tru1I TTAA 2 cut(s) 1623, 1694
Tru9I TTAA 2 cut(s) 1623, 1694
TscAI CASTG 5 cut(s) 114, 268, 904, 1296, 1422
TseFI GTSAC 1 cut(s) 531
TseI GCWGC 9 cut(s) 152, 282, 285, 288, 430, 545, 989, 992, 1378
Tsp45I GTSAC 1 cut(s) 531
TspDTI ATGAA 7 cut(s) 13, 78, 540, 676, 753, 996, 1121
TspGWI ACGGA 3 cut(s) 54, 597, 605
TspRI CASTG 5 cut(s) 114, 268, 904, 1296, 1422
VpaK11BI GGWCC 2 cut(s) 348, 613
XceI RCATGY 5 cut(s) 406, 797, 1005, 1078, 1482
XmiI GTMKAC 1 cut(s) 1681
XspI CTAG 3 cut(s) 852, 1179, 1305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.