pycom12g14500

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0002672
Physical Location & Seq
Forward (+)
17029160 .. 17031349
2190 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1044 bp
ATGAAATTTTTCCACTCAACCATACCTAACATTATTGGGTTGTGGATTACTTATGGTTTTGGGCTGACATTGATTCTCAACATTCAGAACATACTGAAAGCAACTGAGTTGCAGTTCCCTTCAGACAGCAACAATTTGAGTTCAGAGTGCAAGGAATTGTGTCAGAAATTGTTACGCCGCAATCCAGTGGAACGCCTAACATTTGAAGAGTTCTTTGATCACCCATTTCTTTCTCAACAGTCACCATATTCAAGGAGTAGGAGGGCATCAAGAATGGTGGATGGACTTCCTACATCTGAATGCAATGCAATGACGAACATGGAGGAAAGTTCTCAAGAGGATTGTCTGCCTTTCCATTTAGATGATGATTCCAGTGGCCCTGAGGGGAGCCCATCCTTTTCAAGGAGGAGGTCCTCGATGAAGTACACTTATGGATTTTCTCTCGATACAAAAGGTGATAGACGGGAAGCATCACCTACCACTTCGTACAGATATGGCAGTGCAACACGTAAAGAAAATTCTAGTCTTAGGTTGGACGGCCATAGACCATTAGATAGAAATCTAACTGACCCTCTAAGATTTGTGCACCAAAAACCAGTGAATGCTCGATCAAGAGTTATGGATTCGCTGGAGTTGATTGATCAAGATTATGTTCTTGTGTCTGGACCTTCAGTTGATGTGTCTTCTTCCTCAGCTAGTGCTTCCAAACCAAGCCCTTCTTTATACAAATCACAGAGTCTCCCTCGAGAATCTTTAGCCACCTCATCAAGTGCCCCAATGCCAATCAAGGGTGCAGCTGCTAATAGTAACGTGTGTTGCATAAGAAGCTTGGATAGTCAGAGTTCTGCTCCAGGGACGGCACTTGGATCAACGGATATGGGAGATGCGTTGGAGCAGCCATCAACACACTGCATGACAAGGCTTAGTTCGTTGCAGCAATGTGTGTCTGCTATTACTGAATTAGATCATGAAAATGTAAATCATGAGATGCCCACTTGTATGAAGTTGTTTATGGTTCCCTCTGTAAAATCAGTAACTATGTAG

Protein Analysis

348

Amino Acids

38.42

Weight (kDa)

6.26

Isoelectric Point (pI)

75.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIT_ATG1 PF24497 289 - 329 2.4e-11 ATG1, MIT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 178
AclWI GGATC 1 cut(s) 874
AcoI YGGCCR 1 cut(s) 538
AcsI RAATTY 2 cut(s) 5, 517
AcuI CTGAAG 2 cut(s) 105, 654
AfaI GTAC 2 cut(s) 425, 488
AfiI CCNNNNNNNGG 2 cut(s) 402, 788
AflIII ACRYGT 2 cut(s) 506, 810
AgsI TTSAA 3 cut(s) 206, 252, 402
AjnI CCWGG 1 cut(s) 850
AluBI AGCT 3 cut(s) 695, 797, 828
AluI AGCT 3 cut(s) 695, 797, 828
Alw21I GWGCWC 1 cut(s) 588
Alw26I GTCTC 1 cut(s) 743
Alw44I GTGCAC 1 cut(s) 584
AlwI GGATC 1 cut(s) 874
Ama87I CYCGRG 1 cut(s) 744
AoxI GGCC 2 cut(s) 376, 538
ApaLI GTGCAC 1 cut(s) 584
ApeKI GCWGC 4 cut(s) 794, 797, 895, 934
ApoI RAATTY 2 cut(s) 5, 517
Asp700I GAANNNNTTC 1 cut(s) 8
AspS9I GGNCC 3 cut(s) 377, 411, 665
AsuHPI GGTGA 4 cut(s) 212, 234, 465, 467
AvaI CYCGRG 1 cut(s) 744
AvaII GGWCC 2 cut(s) 411, 665
AxyI CCTNAGG 1 cut(s) 381
BaeGI GKGCMC 2 cut(s) 588, 775
BanII GRGCYC 1 cut(s) 392
BbsI GAAGAC 1 cut(s) 675
Bbv12I GWGCWC 1 cut(s) 588
BbvCI CCTCAGC 1 cut(s) 691
BbvI GCAGC 4 cut(s) 784, 806, 907, 946
BccI CCATC 3 cut(s) 275, 400, 907
BceAI ACGGC 2 cut(s) 553, 873
BciT130I CCWGG 1 cut(s) 852
BclI TGATCA 2 cut(s) 217, 640
BcoDI GTCTC 1 cut(s) 743
BfaI CTAG 2 cut(s) 522, 696
BisI GCNGC 5 cut(s) 178, 795, 798, 896, 935
BlsI GCNGC 5 cut(s) 179, 796, 799, 897, 936
Bme1390I CCNGG 1 cut(s) 852
Bme18I GGWCC 2 cut(s) 411, 665
BmeT110I CYCGRG 1 cut(s) 744
BmgT120I GGNCC 3 cut(s) 377, 411, 665
BmiI GGNNCC 2 cut(s) 389, 1017
BmrFI CCNGG 1 cut(s) 852
BmsI GCATC 4 cut(s) 275, 479, 874, 978
BpiI GAAGAC 1 cut(s) 675
BplI GAGNNNNNCTC 4 cut(s) 727, 759, 832, 864
BpmI CTGGAG 2 cut(s) 650, 834
Bpu10I CCTNAGC 1 cut(s) 691
BpuEI CTTGAG 1 cut(s) 318
BsaAI YACGTR 1 cut(s) 509
BsaBI GATNNNNATC 1 cut(s) 558
BsaJI CCNNGG 1 cut(s) 851
BsaXI ACNNNNNCTCC 2 cut(s) 723, 753
Bsc4I CCNNNNNNNGG 2 cut(s) 402, 788
Bse1I ACTGG 3 cut(s) 185, 372, 596
Bse21I CCTNAGG 1 cut(s) 381
Bse3DI GCAATG 3 cut(s) 310, 315, 944
Bse8I GATNNNNATC 1 cut(s) 558
BseBI CCWGG 1 cut(s) 852
BseDI CCNNGG 1 cut(s) 851
BseGI GGATG 2 cut(s) 286, 392
BseJI GATNNNNATC 1 cut(s) 558
BseLI CCNNNNNNNGG 2 cut(s) 402, 788
BseMI GCAATG 3 cut(s) 310, 315, 944
BseMII CTCAG 3 cut(s) 96, 372, 705
BseNI ACTGG 3 cut(s) 185, 372, 596
BseRI GAGGAG 1 cut(s) 421
BseSI GKGCMC 2 cut(s) 588, 775
BseXI GCAGC 4 cut(s) 784, 806, 907, 946
BsgI GTGCAG 1 cut(s) 813
BshFI GGCC 2 cut(s) 378, 540
BsiHKAI GWGCWC 1 cut(s) 588
BsiHKCI CYCGRG 1 cut(s) 744
BslFI GGGAC 1 cut(s) 868
BslI CCNNNNNNNGG 2 cut(s) 402, 788
BsmAI GTCTC 1 cut(s) 743
BsmFI GGGAC 1 cut(s) 868
BsmI GAATGC 2 cut(s) 305, 607
BsnI GGCC 2 cut(s) 378, 540
BsoBI CYCGRG 1 cut(s) 744
Bsp1286I GDGCHC 3 cut(s) 392, 588, 775
Bsp143I GATC 5 cut(s) 217, 608, 640, 866, 964
BspACI CCGC 1 cut(s) 178
BspANI GGCC 2 cut(s) 378, 540
BspCNI CTCAG 3 cut(s) 97, 373, 704
BspHI TCATGA 2 cut(s) 967, 982
BspLI GGNNCC 2 cut(s) 389, 1017
BspPI GGATC 1 cut(s) 874
BsrDI GCAATG 3 cut(s) 310, 315, 944
BsrI ACTGG 3 cut(s) 185, 372, 596
BssECI CCNNGG 1 cut(s) 851
BssMI GATC 5 cut(s) 217, 608, 640, 866, 964
Bst2UI CCWGG 1 cut(s) 852
Bst4CI ACNGT 1 cut(s) 240
Bst6I CTCTTC 1 cut(s) 201
BstBAI YACGTR 1 cut(s) 509
BstDEI CTNAG 6 cut(s) 105, 381, 527, 575, 691, 923
BstENI CCTNNNNNAGG 1 cut(s) 400
BstF5I GGATG 2 cut(s) 286, 392
BstKTI GATC 5 cut(s) 220, 611, 643, 869, 967
BstMAI GTCTC 1 cut(s) 743
BstMBI GATC 5 cut(s) 217, 608, 640, 866, 964
BstMWI GCNNNNNNNGC 1 cut(s) 825
BstNI CCWGG 1 cut(s) 852
BstSCI CCNGG 1 cut(s) 850
BstSLI GKGCMC 2 cut(s) 588, 775
BstV1I GCAGC 4 cut(s) 784, 806, 907, 946
BstV2I GAAGAC 1 cut(s) 675
Bsu36I CCTNAGG 1 cut(s) 381
BsuRI GGCC 2 cut(s) 378, 540
BtsCI GGATG 2 cut(s) 286, 392
BtsI GCAGTG 2 cut(s) 505, 907
BtsIMutI CAGTG 5 cut(s) 192, 379, 505, 603, 907
CciI TCATGA 2 cut(s) 967, 982
Cfr13I GGNCC 3 cut(s) 377, 411, 665
Csp6I GTAC 2 cut(s) 424, 487
CspCI CAANNNNNGTGG 2 cut(s) 258, 293
CviAII CATG 4 cut(s) 319, 913, 968, 983
CviQI GTAC 2 cut(s) 424, 487
DdeI CTNAG 6 cut(s) 105, 381, 527, 575, 691, 923
DpnI GATC 5 cut(s) 219, 610, 642, 868, 966
DpnII GATC 5 cut(s) 217, 608, 640, 866, 964
EaeI YGGCCR 1 cut(s) 538
Eam1104I CTCTTC 1 cut(s) 201
EarI CTCTTC 1 cut(s) 201
Eco24I GRGCYC 1 cut(s) 392
Eco47I GGWCC 2 cut(s) 411, 665
Eco57I CTGAAG 2 cut(s) 105, 654
Eco81I CCTNAGG 1 cut(s) 381
Eco88I CYCGRG 1 cut(s) 744
EcoNI CCTNNNNNAGG 1 cut(s) 400
EcoO109I RGGNCCY 1 cut(s) 411
EcoRII CCWGG 1 cut(s) 850
EcoT38I GRGCYC 1 cut(s) 392
FaeI CATG 4 cut(s) 322, 916, 971, 986
FalI AAGNNNNNCTT 2 cut(s) 703, 735
FaqI GGGAC 1 cut(s) 868
FatI CATG 4 cut(s) 318, 912, 967, 982
FbaI TGATCA 2 cut(s) 217, 640
Fnu4HI GCNGC 5 cut(s) 178, 795, 798, 896, 935
FokI GGATG 2 cut(s) 293, 379
FriOI GRGCYC 1 cut(s) 392
Fsp4HI GCNGC 5 cut(s) 178, 795, 798, 896, 935
FspBI CTAG 2 cut(s) 522, 696
GluI GCNGC 5 cut(s) 178, 795, 798, 896, 935
GsuI CTGGAG 2 cut(s) 650, 834
HaeIII GGCC 2 cut(s) 378, 540
Hin1II CATG 4 cut(s) 322, 916, 971, 986
HindIII AAGCTT 1 cut(s) 826
HinfI GANTC 5 cut(s) 73, 368, 623, 736, 749
HphI GGTGA 4 cut(s) 212, 234, 465, 467
Hpy166II GTNNAC 2 cut(s) 426, 586
Hpy188I TCNGA 6 cut(s) 87, 124, 145, 165, 298, 840
Hpy188III TCNNGA 9 cut(s) 270, 335, 443, 612, 644, 663, 746, 968, 983
Hpy8I GTNNAC 2 cut(s) 426, 586
HpyAV CCTTC 3 cut(s) 129, 678, 726
HpyCH4III ACNGT 1 cut(s) 240
HpyCH4IV ACGT 2 cut(s) 508, 810
HpyF10VI GCNNNNNNNGC 1 cut(s) 825
HpyF3I CTNAG 6 cut(s) 105, 381, 527, 575, 691, 923
HpySE526I ACGT 2 cut(s) 508, 810
Hsp92II CATG 4 cut(s) 322, 916, 971, 986
Ksp22I TGATCA 2 cut(s) 217, 640
Kzo9I GATC 5 cut(s) 217, 608, 640, 866, 964
LmnI GCTCC 3 cut(s) 387, 853, 892
LpnPI CCDG 8 cut(s) 198, 385, 393, 609, 614, 648, 837, 864
Lsp1109I GCAGC 4 cut(s) 784, 806, 907, 946
LweI GCATC 4 cut(s) 275, 479, 874, 978
MaeI CTAG 2 cut(s) 522, 696
MaeII ACGT 2 cut(s) 508, 810
MaeIII GTNAC 4 cut(s) 171, 240, 806, 1033
MalI GATC 5 cut(s) 219, 610, 642, 868, 966
MboI GATC 5 cut(s) 217, 608, 640, 866, 964
MboII GAAGA 3 cut(s) 218, 675, 678
MhlI GDGCHC 3 cut(s) 392, 588, 775
MluCI AATT 6 cut(s) 5, 133, 155, 167, 517, 959
MlyI GAGTC 1 cut(s) 745
MmeI TCCRAC 2 cut(s) 513, 870
MroXI GAANNNNTTC 1 cut(s) 8
MslI CAYNNNNRTG 4 cut(s) 298, 360, 972, 998
MspA1I CMGCKG 1 cut(s) 797
MspR9I CCNGG 1 cut(s) 852
Mva1269I GAATGC 2 cut(s) 305, 607
MvaI CCWGG 1 cut(s) 852
MwoI GCNNNNNNNGC 1 cut(s) 825
NdeII GATC 5 cut(s) 217, 608, 640, 866, 964
NlaIII CATG 4 cut(s) 322, 916, 971, 986
NlaIV GGNNCC 2 cut(s) 389, 1017
NmuCI GTSAC 1 cut(s) 240
PaeR7I CTCGAG 1 cut(s) 744
PagI TCATGA 2 cut(s) 967, 982
PctI GAATGC 2 cut(s) 305, 607
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 4 cut(s) 73, 368, 623, 749
PkrI GCNGC 5 cut(s) 179, 796, 799, 897, 936
PleI GAGTC 1 cut(s) 744
PpsI GAGTC 1 cut(s) 744
Ppu21I YACGTR 1 cut(s) 509
PpuMI RGGWCCY 1 cut(s) 411
Psp5II RGGWCCY 1 cut(s) 411
Psp6I CCWGG 1 cut(s) 850
PspGI CCWGG 1 cut(s) 850
PspN4I GGNNCC 2 cut(s) 389, 1017
PspPI GGNCC 3 cut(s) 377, 411, 665
PspPPI RGGWCCY 1 cut(s) 411
PvuII CAGCTG 1 cut(s) 797
RsaI GTAC 2 cut(s) 425, 488
RsaNI GTAC 2 cut(s) 424, 487
RseI CAYNNNNRTG 4 cut(s) 298, 360, 972, 998
SatI GCNGC 5 cut(s) 178, 795, 798, 896, 935
Sau3AI GATC 5 cut(s) 217, 608, 640, 866, 964
Sau96I GGNCC 3 cut(s) 377, 411, 665
SchI GAGTC 1 cut(s) 745
ScrFI CCNGG 1 cut(s) 852
SduI GDGCHC 3 cut(s) 392, 588, 775
SfaNI GCATC 4 cut(s) 275, 479, 874, 978
Sfr274I CTCGAG 1 cut(s) 744
SinI GGWCC 2 cut(s) 411, 665
SlaI CTCGAG 1 cut(s) 744
SmiMI CAYNNNNRTG 4 cut(s) 298, 360, 972, 998
SmlI CTYRAG 2 cut(s) 333, 744
SmoI CTYRAG 2 cut(s) 333, 744
Sse9I AATT 6 cut(s) 5, 133, 155, 167, 517, 959
SsiI CCGC 1 cut(s) 178
SspMI CTAG 2 cut(s) 522, 696
StyD4I CCNGG 1 cut(s) 850
TaaI ACNGT 1 cut(s) 240
TaiI ACGT 2 cut(s) 511, 813
TaqI TCGA 4 cut(s) 416, 444, 607, 745
TasI AATT 6 cut(s) 5, 133, 155, 167, 517, 959
TatI WGTACW 1 cut(s) 423
TauI GCSGC 1 cut(s) 180
TfiI GAWTC 4 cut(s) 73, 368, 623, 749
TscAI CASTG 5 cut(s) 192, 379, 505, 603, 914
TseFI GTSAC 1 cut(s) 240
TseI GCWGC 4 cut(s) 794, 797, 895, 934
Tsp45I GTSAC 1 cut(s) 240
TspDTI ATGAA 4 cut(s) 17, 434, 984, 1016
TspGWI ACGGA 1 cut(s) 887
TspRI CASTG 5 cut(s) 192, 379, 505, 603, 914
VneI GTGCAC 1 cut(s) 584
VpaK11BI GGWCC 2 cut(s) 411, 665
XagI CCTNNNNNAGG 1 cut(s) 400
XapI RAATTY 2 cut(s) 5, 517
XhoI CTCGAG 1 cut(s) 744
XmnI GAANNNNTTC 1 cut(s) 8
XspI CTAG 2 cut(s) 522, 696
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.