pycom12g17530

Plant non-specific lipid-transfer proteins transfer phospholipids as well as galactolipids across membranes. May play a role in wax or cutin deposition in the cell walls of expanding epidermal cells and certain secretory tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0005968
Physical Location & Seq
Forward (+)
19916030 .. 19916548
519 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 351 bp
ATGGCTAGTCCTAGGGTGGTTTGTGTTTTCTTAATGTGGATAGTGCTGGCTGCACCCTTGATCACTGGAGCAGCACTCACATGTGGCCAGATTAAAGGCGATCTTGCGCCTTGCCTGGCCTACCTCCAGAATGGTGGCTCCCCCACTCCGGGCTGCTGCAGGGGCATCAAAGGCCTGGTCAGCTCAGCCACGACCACAGCTGATCGACAGAATGCTTGCAAATGCTTGAAAACAGTTGCTGCCCAAATCAAAACTATTAAACAAGCCAACGCGGCTAAACTCCCTTCCTTATGTGGCGTCAACATTCCCTACAAGATCAGCACCTCTACTAACTGCGCTGGTGTGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tryp_alpha_amyl

Protein Analysis

117

Amino Acids

11.88

Weight (kDa)

9.44

Isoelectric Point (pI)

28.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LTP_2 PF14368 12 - 104 2.1e-08 Probable lipid transfer
Tryp_alpha_amyl PF00234 28 - 112 7.1e-14 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 272
AciI CCGC 1 cut(s) 272
AcoI YGGCCR 1 cut(s) 85
AcyI GRCGYC 1 cut(s) 297
AfiI CCNNNNNNNGG 2 cut(s) 148, 149
AflIII ACRYGT 1 cut(s) 80
AgsI TTSAA 1 cut(s) 229
AjnI CCWGG 2 cut(s) 114, 174
AluBI AGCT 2 cut(s) 183, 200
AluI AGCT 2 cut(s) 183, 200
AlwNI CAGNNNCTG 1 cut(s) 239
AoxI GGCC 3 cut(s) 85, 117, 172
ApeKI GCWGC 5 cut(s) 50, 71, 153, 156, 239
ArsI GACNNNNNNTTYG 2 cut(s) 162, 194
AspA2I CCTAGG 1 cut(s) 11
AspLEI GCGC 2 cut(s) 109, 338
AsuC2I CCSGG 1 cut(s) 150
AvrII CCTAGG 1 cut(s) 11
BalI TGGCCA 1 cut(s) 87
BbvI GCAGC 5 cut(s) 37, 83, 140, 143, 226
BciT130I CCWGG 2 cut(s) 116, 176
BclI TGATCA 1 cut(s) 60
BcnI CCSGG 1 cut(s) 150
BfaI CTAG 2 cut(s) 6, 12
BfmI CTRYAG 1 cut(s) 157
BglI GCCNNNNNGGC 1 cut(s) 272
BisI GCNGC 6 cut(s) 51, 72, 154, 157, 240, 273
BlnI CCTAGG 1 cut(s) 11
BlpI GCTNAGC 1 cut(s) 184
BlsI GCNGC 6 cut(s) 52, 73, 155, 158, 241, 274
Bme1390I CCNGG 3 cut(s) 116, 150, 176
BmiI GGNNCC 1 cut(s) 139
BmrFI CCNGG 3 cut(s) 116, 150, 176
BmsI GCATC 1 cut(s) 174
BplI GAGNNNNNCTC 2 cut(s) 60, 92
BpmI CTGGAG 2 cut(s) 87, 110
Bpu1102I GCTNAGC 1 cut(s) 184
BpuMI CCSGG 1 cut(s) 150
BsaHI GRCGYC 1 cut(s) 297
BsaJI CCNNGG 1 cut(s) 11
Bsc4I CCNNNNNNNGG 2 cut(s) 148, 149
Bse1I ACTGG 1 cut(s) 70
BseBI CCWGG 2 cut(s) 116, 176
BseDI CCNNGG 1 cut(s) 11
BseLI CCNNNNNNNGG 2 cut(s) 148, 149
BseMII CTCAG 1 cut(s) 198
BseNI ACTGG 1 cut(s) 70
BseXI GCAGC 5 cut(s) 37, 83, 140, 143, 226
BsgI GTGCAG 1 cut(s) 36
Bsh1236I CGCG 1 cut(s) 272
BshFI GGCC 3 cut(s) 87, 119, 174
BsiSI CCGG 1 cut(s) 149
BslI CCNNNNNNNGG 2 cut(s) 148, 149
BsmI GAATGC 1 cut(s) 217
BsnI GGCC 3 cut(s) 87, 119, 174
Bsp143I GATC 4 cut(s) 60, 100, 202, 315
Bsp1720I GCTNAGC 1 cut(s) 184
BspACI CCGC 1 cut(s) 272
BspANI GGCC 3 cut(s) 87, 119, 174
BspCNI CTCAG 1 cut(s) 197
BspFNI CGCG 1 cut(s) 272
BspLI GGNNCC 1 cut(s) 139
BspMAI CTGCAG 1 cut(s) 161
BsrI ACTGG 1 cut(s) 70
BssECI CCNNGG 1 cut(s) 11
BssMI GATC 4 cut(s) 60, 100, 202, 315
BssNI GRCGYC 1 cut(s) 297
BssT1I CCWWGG 1 cut(s) 11
Bst2UI CCWGG 2 cut(s) 116, 176
Bst4CI ACNGT 1 cut(s) 235
BstACI GRCGYC 1 cut(s) 297
BstC8I GCNNGC 2 cut(s) 48, 217
BstDEI CTNAG 1 cut(s) 184
BstFNI CGCG 1 cut(s) 272
BstHHI GCGC 2 cut(s) 109, 338
BstKTI GATC 4 cut(s) 63, 103, 205, 318
BstMBI GATC 4 cut(s) 60, 100, 202, 315
BstMWI GCNNNNNNNGC 4 cut(s) 162, 171, 180, 272
BstNI CCWGG 2 cut(s) 116, 176
BstNSI RCATGY 1 cut(s) 84
BstSCI CCNGG 3 cut(s) 114, 148, 174
BstSFI CTRYAG 1 cut(s) 157
BstUI CGCG 1 cut(s) 272
BstV1I GCAGC 5 cut(s) 37, 83, 140, 143, 226
BstXI CCANNNNNNTGG 1 cut(s) 134
BsuRI GGCC 3 cut(s) 87, 119, 174
BtsIMutI CAGTG 1 cut(s) 63
Cac8I GCNNGC 2 cut(s) 48, 217
CaiI CAGNNNCTG 1 cut(s) 239
CfoI GCGC 2 cut(s) 109, 338
CseI GACGC 1 cut(s) 286
CviAII CATG 1 cut(s) 81
DdeI CTNAG 1 cut(s) 184
DpnI GATC 4 cut(s) 62, 102, 204, 317
DpnII GATC 4 cut(s) 60, 100, 202, 315
EaeI YGGCCR 1 cut(s) 85
Eco130I CCWWGG 1 cut(s) 11
Eco147I AGGCCT 1 cut(s) 174
EcoRII CCWGG 2 cut(s) 114, 174
EcoT14I CCWWGG 1 cut(s) 11
ErhI CCWWGG 1 cut(s) 11
FaeI CATG 1 cut(s) 84
FaiI YATR 2 cut(s) 82, 292
FalI AAGNNNNNCTT 2 cut(s) 87, 119
FatI CATG 1 cut(s) 80
FbaI TGATCA 1 cut(s) 60
Fnu4HI GCNGC 6 cut(s) 51, 72, 154, 157, 240, 273
Fsp4HI GCNGC 6 cut(s) 51, 72, 154, 157, 240, 273
FspBI CTAG 2 cut(s) 6, 12
GlaI GCGC 2 cut(s) 108, 337
GluI GCNGC 6 cut(s) 51, 72, 154, 157, 240, 273
GsuI CTGGAG 2 cut(s) 87, 110
HaeIII GGCC 3 cut(s) 87, 119, 174
HapII CCGG 1 cut(s) 149
HgaI GACGC 1 cut(s) 286
HhaI GCGC 2 cut(s) 109, 338
Hin1I GRCGYC 1 cut(s) 297
Hin1II CATG 1 cut(s) 84
Hin6I GCGC 2 cut(s) 107, 336
HinP1I GCGC 2 cut(s) 107, 336
HincII GTYRAC 1 cut(s) 301
HindII GTYRAC 1 cut(s) 301
HpaII CCGG 1 cut(s) 149
Hpy166II GTNNAC 1 cut(s) 301
Hpy188III TCNNGA 1 cut(s) 127
Hpy8I GTNNAC 1 cut(s) 301
HpyAV CCTTC 1 cut(s) 294
HpyCH4III ACNGT 1 cut(s) 235
HpyCH4V TGCA 3 cut(s) 53, 159, 219
HpyF10VI GCNNNNNNNGC 4 cut(s) 162, 171, 180, 272
HpyF3I CTNAG 1 cut(s) 184
Hsp92I GRCGYC 1 cut(s) 297
Hsp92II CATG 1 cut(s) 84
HspAI GCGC 2 cut(s) 107, 336
Ksp22I TGATCA 1 cut(s) 60
Kzo9I GATC 4 cut(s) 60, 100, 202, 315
LmnI GCTCC 2 cut(s) 68, 143
Lsp1109I GCAGC 5 cut(s) 37, 83, 140, 143, 226
LweI GCATC 1 cut(s) 174
MaeI CTAG 2 cut(s) 6, 12
MalI GATC 4 cut(s) 62, 102, 204, 317
MboI GATC 4 cut(s) 60, 100, 202, 315
MlsI TGGCCA 1 cut(s) 87
MluNI TGGCCA 1 cut(s) 87
MnlI CCTC 2 cut(s) 134, 334
Mox20I TGGCCA 1 cut(s) 87
MscI TGGCCA 1 cut(s) 87
MseI TTAA 3 cut(s) 32, 93, 258
MslI CAYNNNNRTG 1 cut(s) 79
Msp20I TGGCCA 1 cut(s) 87
MspA1I CMGCKG 1 cut(s) 200
MspI CCGG 1 cut(s) 149
MspR9I CCNGG 3 cut(s) 116, 150, 176
Mva1269I GAATGC 1 cut(s) 217
MvaI CCWGG 2 cut(s) 116, 176
MvnI CGCG 1 cut(s) 272
MwoI GCNNNNNNNGC 4 cut(s) 162, 171, 180, 272
NciI CCSGG 1 cut(s) 150
NdeII GATC 4 cut(s) 60, 100, 202, 315
NlaIII CATG 1 cut(s) 84
NlaIV GGNNCC 1 cut(s) 139
NspI RCATGY 1 cut(s) 84
PceI AGGCCT 1 cut(s) 174
PciI ACATGT 1 cut(s) 80
PctI GAATGC 1 cut(s) 217
PkrI GCNGC 6 cut(s) 52, 73, 155, 158, 241, 274
PscI ACATGT 1 cut(s) 80
Psp6I CCWGG 2 cut(s) 114, 174
PspGI CCWGG 2 cut(s) 114, 174
PspN4I GGNNCC 1 cut(s) 139
PstI CTGCAG 1 cut(s) 161
PstNI CAGNNNCTG 1 cut(s) 239
PvuII CAGCTG 1 cut(s) 200
RseI CAYNNNNRTG 1 cut(s) 79
SaqAI TTAA 3 cut(s) 32, 93, 258
SatI GCNGC 6 cut(s) 51, 72, 154, 157, 240, 273
Sau3AI GATC 4 cut(s) 60, 100, 202, 315
ScrFI CCNGG 3 cut(s) 116, 150, 176
SetI ASST 4 cut(s) 126, 185, 202, 326
SfaNI GCATC 1 cut(s) 174
SfcI CTRYAG 1 cut(s) 157
SmiMI CAYNNNNRTG 1 cut(s) 79
SseBI AGGCCT 1 cut(s) 174
SsiI CCGC 1 cut(s) 272
SspMI CTAG 2 cut(s) 6, 12
StuI AGGCCT 1 cut(s) 174
StyD4I CCNGG 3 cut(s) 114, 148, 174
StyI CCWWGG 1 cut(s) 11
TaaI ACNGT 1 cut(s) 235
TaqI TCGA 1 cut(s) 205
TauI GCSGC 1 cut(s) 275
Tru1I TTAA 3 cut(s) 32, 93, 258
Tru9I TTAA 3 cut(s) 32, 93, 258
TscAI CASTG 1 cut(s) 70
TseI GCWGC 5 cut(s) 50, 71, 153, 156, 239
TspRI CASTG 1 cut(s) 70
XceI RCATGY 1 cut(s) 84
XmaJI CCTAGG 1 cut(s) 11
XspI CTAG 2 cut(s) 6, 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.