pycom12g17610

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0011413
Physical Location & Seq
Forward (+)
20002468 .. 20006999
4532 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1365 bp
ATGGCTACGTCACCTTCGTGTGATGGCCAGCACGCCCTGATCTCGGTCGGGGAATCCCAAAGCTCTCATGACTGTCTGCAAAGAATTATATGTTACCACATCCACATCGTATCACAGGTGGTACACTTGGGCAAGGGCAAGGAGGGTTCTCCGGTGCCTAGTCCGCTGCTGCCTCCTACGGTGTCAAGTTCTGGTGAAAATGGGTCGGATACTAAGCCGGTTGATGACCCGAGTAAGGAGAAAGTGGAGTCTTCGCAACCCGCGAAGACTCCGAAACCAAAAGTGGATGAGGGCCCCAAGGTGGGTTTGAACAATGATACTAAAAGGGGTGAAGGGAAGGATGTGGAAACAGAAATGAAGGATCCAGAGCCTGCCAAGACATCGAATGATGATCCGAAGCCGGATACCGACAAGGTGAAAGATAATCCAAAGGAGGACACTGACAAGGTGTTTAAAGATAATCCTAAGGAGAAGGAGAAAGAGAGGGAGAAGGAGAAGGAGAAGGATAATCCGAAGGAGAAGGAGAAGGAGAAGGAGAAGGATAATCCGAAGGAGAAGGAGAAGGAGAAGGATAATCCAAAGGAGAAGGGGAAGGACAATCCAAAGGAGAAGGAGAAGGATAATCCAAAGGAGAAGGAGAAGGAGAAGGATAATCCAAAGGAGAAGGGGAAGGATAATCCGAAGGAGAAGGAGAAGGAGAAGGATAATCCAAAGGAGAAGGAGAAGGAGAAGGAGAAGCAGGAGAGCTGCGCTGGGGTAACCAAAATCAAAACATGTGCGGTCAAGGATGTGGTGGTTGCTTGCATTAAGAGCTTCAGTAGTGGGTCCAAAGAAGTGGCCATTCTGGTACAAAACGTAGGAAACAGCACTTTGAAGGCAACCCTTTCTGCAAATGATACCAGGAAGCACCTAGAGATTCACAAACACCAAAACGGAAAGGTCAATATATCAATTGATCCGGGTAAAAGCACCAATATAACATTAAATGCTGGAACCGGAGAATGTAAGCTTCACATAGATCCTCCCCCTTGGGCGTTTCCTTCTCTCAAGACCATAGCGACCCCAATCAACGGTGCCTACATCTTGATTGTTGCAGTGCTAACTTTTGGAGGAATGTGGGCTTGCTGCTTGATCAGGAAGAGGAAAGAGCGTACTGGAGGTGGAGTTCCATATCAGGAACTTGAAATGGGATTGCCGGAATCTGTTCCAGCAACTGCCGTGGAAACAGCTGAAGGTTGGGATGAAGGTTGGGATGATGACTGGGACGGAGAAAATGCGGTGAAAACACCTGGGGCGCATCTGGGAAGCATCTCTGTGAATGGCCTCACATCGAGGGCTGCAAACAAAGATGGGTGGAATGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

455

Amino Acids

49.82

Weight (kDa)

6.06

Isoelectric Point (pI)

34.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7356 PF24053 242 - 342 1.3e-25 Domain of unknown function (DUF7356)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011413)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G51580 AT3G51580
fragaria_vesca FvH4_6g09850
malus_domestica MD04G1174800.v1.1 MD12G1188500.v1.1
prunus_persica Prupe.6G293800_v2.0.a1
pyrus_communis pycom04g15420 pycom12g17610
rosa_chinensis RchiOBHm_Chr3g0460451
rosa_laevigata RLG00000025010
rosa_multiflora Rmu_sc0004267.1_g000009 Rmu_sc0021028.1_g000003
rosa_roxburghii Rroxscaffold_6G00419470
rosa_rugosa Rorug03G0042600 Rorug03G0042700
rosa_samantha Rh3AG100800 Rh3BG104800 Rh3CG106600 Rh3DG106100
rosa_wichuraiana Rw3G008580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 154, 1073
AccII CGCG 1 cut(s) 263
AciI CCGC 4 cut(s) 164, 261, 779, 1277
AclWI GGATC 5 cut(s) 356, 369, 386, 950, 1013
AcoI YGGCCR 2 cut(s) 25, 837
AcuI CTGAAG 2 cut(s) 799, 1251
AfaI GTAC 3 cut(s) 123, 849, 1153
AfiI CCNNNNNNNGG 5 cut(s) 43, 235, 301, 302, 1070
AflIII ACRYGT 1 cut(s) 773
AgsI TTSAA 3 cut(s) 310, 874, 1184
AjnI CCWGG 2 cut(s) 899, 1288
AleI CACNNNNGTG 1 cut(s) 16
AloI GAACNNNNNNTCC 2 cut(s) 1149, 1181
AluBI AGCT 5 cut(s) 63, 747, 813, 1009, 1229
AluI AGCT 5 cut(s) 63, 747, 813, 1009, 1229
AlwI GGATC 5 cut(s) 356, 369, 386, 950, 1013
AlwNI CAGNNNCTG 2 cut(s) 371, 1214
Ama87I CYCGRG 1 cut(s) 229
AoxI GGCC 4 cut(s) 25, 292, 837, 1321
ApaI GGGCCC 1 cut(s) 296
ApeKI GCWGC 5 cut(s) 166, 169, 747, 1125, 1337
Asp700I GAANNNNTTC 1 cut(s) 1203
AspLEI GCGC 2 cut(s) 752, 1297
AspS9I GGNCC 3 cut(s) 292, 293, 825
AsuC2I CCSGG 1 cut(s) 960
AsuHPI GGTGA 5 cut(s) 3, 206, 341, 427, 1291
AvaI CYCGRG 1 cut(s) 229
AvaII GGWCC 1 cut(s) 825
AxyI CCTNAGG 1 cut(s) 465
BaeGI GKGCMC 1 cut(s) 296
BalI TGGCCA 2 cut(s) 27, 839
BamHI GGATCC 1 cut(s) 361
BanI GGYRCC 2 cut(s) 154, 1073
BanII GRGCYC 1 cut(s) 296
BbsI GAAGAC 2 cut(s) 243, 272
BbvI GCAGC 5 cut(s) 153, 156, 734, 1112, 1324
BccI CCATC 2 cut(s) 17, 1343
BceAI ACGGC 1 cut(s) 1202
BciT130I CCWGG 2 cut(s) 901, 1290
BciVI GTATCC 2 cut(s) 202, 397
BclI TGATCA 1 cut(s) 1131
BcnI CCSGG 1 cut(s) 960
BfaI CTAG 2 cut(s) 159, 911
BfuI GTATCC 2 cut(s) 202, 397
BisI GCNGC 5 cut(s) 167, 170, 748, 1126, 1338
BlsI GCNGC 5 cut(s) 168, 171, 749, 1127, 1339
Bme1390I CCNGG 3 cut(s) 901, 960, 1290
Bme18I GGWCC 1 cut(s) 825
BmeT110I CYCGRG 1 cut(s) 229
BmgT120I GGNCC 3 cut(s) 292, 293, 825
BmiI GGNNCC 7 cut(s) 156, 294, 295, 363, 826, 994, 1075
BmrFI CCNGG 3 cut(s) 901, 960, 1290
BmrI ACTGGG 1 cut(s) 1270
BmsI GCATC 2 cut(s) 1306, 1317
BmuI ACTGGG 1 cut(s) 1270
BpiI GAAGAC 2 cut(s) 243, 272
BpmI CTGGAG 1 cut(s) 1176
BpuEI CTTGAG 1 cut(s) 1031
BpuMI CCSGG 1 cut(s) 960
BsaJI CCNNGG 4 cut(s) 297, 1028, 1218, 1289
BsaWI WCCGGW 2 cut(s) 151, 995
BsaXI ACNNNNNCTCC 2 cut(s) 1149, 1179
Bsc4I CCNNNNNNNGG 5 cut(s) 43, 235, 301, 302, 1070
Bse118I RCCGGY 1 cut(s) 217
Bse1I ACTGG 2 cut(s) 1159, 1265
Bse21I CCTNAGG 1 cut(s) 465
BseBI CCWGG 2 cut(s) 901, 1290
BseDI CCNNGG 4 cut(s) 297, 1028, 1218, 1289
BseGI GGATG 6 cut(s) 99, 292, 346, 793, 1246, 1258
BseLI CCNNNNNNNGG 5 cut(s) 43, 235, 301, 302, 1070
BseNI ACTGG 2 cut(s) 1159, 1265
BseSI GKGCMC 1 cut(s) 296
BseXI GCAGC 5 cut(s) 153, 156, 734, 1112, 1324
BseYI CCCAGC 1 cut(s) 752
Bsh1236I CGCG 1 cut(s) 263
Bsh1285I CGRYCG 1 cut(s) 48
BshFI GGCC 4 cut(s) 27, 294, 839, 1323
BshNI GGYRCC 2 cut(s) 154, 1073
BsiEI CGRYCG 1 cut(s) 48
BsiHKCI CYCGRG 1 cut(s) 229
BsiSI CCGG 6 cut(s) 152, 218, 401, 959, 996, 1196
BslFI GGGAC 1 cut(s) 1277
BslI CCNNNNNNNGG 5 cut(s) 43, 235, 301, 302, 1070
BsmFI GGGAC 1 cut(s) 1277
BsnI GGCC 4 cut(s) 27, 294, 839, 1323
BsoBI CYCGRG 1 cut(s) 229
Bsp120I GGGCCC 1 cut(s) 292
Bsp1286I GDGCHC 1 cut(s) 296
Bsp143I GATC 6 cut(s) 39, 361, 391, 955, 1018, 1131
BspACI CCGC 4 cut(s) 164, 261, 779, 1277
BspANI GGCC 4 cut(s) 27, 294, 839, 1323
BspFNI CGCG 1 cut(s) 263
BspHI TCATGA 1 cut(s) 67
BspLI GGNNCC 7 cut(s) 156, 294, 295, 363, 826, 994, 1075
BspPI GGATC 5 cut(s) 356, 369, 386, 950, 1013
BspT107I GGYRCC 2 cut(s) 154, 1073
BsrFI RCCGGY 1 cut(s) 217
BsrI ACTGG 2 cut(s) 1159, 1265
BssAI RCCGGY 1 cut(s) 217
BssECI CCNNGG 4 cut(s) 297, 1028, 1218, 1289
BssMI GATC 6 cut(s) 39, 361, 391, 955, 1018, 1131
BssT1I CCWWGG 2 cut(s) 297, 1028
Bst2UI CCWGG 2 cut(s) 901, 1290
Bst4CI ACNGT 3 cut(s) 74, 181, 1073
Bst6I CTCTTC 1 cut(s) 1133
BstC8I GCNNGC 5 cut(s) 29, 33, 372, 802, 1123
BstDEI CTNAG 2 cut(s) 213, 465
BstDSI CCRYGG 1 cut(s) 1218
BstEII GGTNACC 1 cut(s) 757
BstF5I GGATG 6 cut(s) 99, 292, 346, 793, 1246, 1258
BstFNI CGCG 1 cut(s) 263
BstHHI GCGC 2 cut(s) 752, 1297
BstKTI GATC 6 cut(s) 42, 364, 394, 958, 1021, 1134
BstMBI GATC 6 cut(s) 39, 361, 391, 955, 1018, 1131
BstMCI CGRYCG 1 cut(s) 48
BstMWI GCNNNNNNNGC 2 cut(s) 163, 810
BstNI CCWGG 2 cut(s) 901, 1290
BstNSI RCATGY 1 cut(s) 777
BstPI GGTNACC 1 cut(s) 757
BstSCI CCNGG 3 cut(s) 899, 958, 1288
BstSLI GKGCMC 1 cut(s) 296
BstUI CGCG 1 cut(s) 263
BstV1I GCAGC 5 cut(s) 153, 156, 734, 1112, 1324
BstV2I GAAGAC 2 cut(s) 243, 272
BstX2I RGATCY 2 cut(s) 361, 1018
BstXI CCANNNNNNTGG 1 cut(s) 835
BstYI RGATCY 2 cut(s) 361, 1018
Bsu36I CCTNAGG 1 cut(s) 465
BsuI GTATCC 2 cut(s) 202, 397
BsuRI GGCC 4 cut(s) 27, 294, 839, 1323
BtgI CCRYGG 1 cut(s) 1218
BtsCI GGATG 6 cut(s) 99, 292, 346, 793, 1246, 1258
BtsI GCAGTG 1 cut(s) 1101
BtsIMutI CAGTG 2 cut(s) 438, 1101
Cac8I GCNNGC 5 cut(s) 29, 33, 372, 802, 1123
CaiI CAGNNNCTG 2 cut(s) 371, 1214
CciI TCATGA 1 cut(s) 67
CfoI GCGC 2 cut(s) 752, 1297
Cfr10I RCCGGY 1 cut(s) 217
Cfr13I GGNCC 3 cut(s) 292, 293, 825
Csp6I GTAC 3 cut(s) 122, 848, 1152
CspCI CAANNNNNGTGG 2 cut(s) 1200, 1235
CviAII CATG 2 cut(s) 68, 774
CviQI GTAC 3 cut(s) 122, 848, 1152
DdeI CTNAG 2 cut(s) 213, 465
DpnI GATC 6 cut(s) 41, 363, 393, 957, 1020, 1133
DpnII GATC 6 cut(s) 39, 361, 391, 955, 1018, 1131
DraI TTTAAA 1 cut(s) 454
EaeI YGGCCR 2 cut(s) 25, 837
Eam1104I CTCTTC 1 cut(s) 1133
EarI CTCTTC 1 cut(s) 1133
Eco130I CCWWGG 2 cut(s) 297, 1028
Eco24I GRGCYC 1 cut(s) 296
Eco47I GGWCC 1 cut(s) 825
Eco57I CTGAAG 2 cut(s) 799, 1251
Eco81I CCTNAGG 1 cut(s) 465
Eco88I CYCGRG 1 cut(s) 229
Eco91I GGTNACC 1 cut(s) 757
EcoO109I RGGNCCY 2 cut(s) 292, 293
EcoO65I GGTNACC 1 cut(s) 757
EcoRII CCWGG 2 cut(s) 899, 1288
EcoT14I CCWWGG 2 cut(s) 297, 1028
EcoT38I GRGCYC 1 cut(s) 296
ErhI CCWWGG 2 cut(s) 297, 1028
FaeI CATG 2 cut(s) 71, 777
FaiI YATR 9 cut(s) 69, 89, 91, 775, 947, 977, 1016, 1055, 1171
FaqI GGGAC 1 cut(s) 1277
FatI CATG 2 cut(s) 67, 773
FauI CCCGC 1 cut(s) 268
FbaI TGATCA 1 cut(s) 1131
Fnu4HI GCNGC 5 cut(s) 167, 170, 748, 1126, 1338
FokI GGATG 6 cut(s) 86, 299, 353, 800, 1253, 1265
FriOI GRGCYC 1 cut(s) 296
Fsp4HI GCNGC 5 cut(s) 167, 170, 748, 1126, 1338
FspBI CTAG 2 cut(s) 159, 911
GlaI GCGC 2 cut(s) 751, 1296
GluI GCNGC 5 cut(s) 167, 170, 748, 1126, 1338
GsaI CCCAGC 1 cut(s) 756
GsuI CTGGAG 1 cut(s) 1176
HaeIII GGCC 4 cut(s) 27, 294, 839, 1323
HapII CCGG 6 cut(s) 152, 218, 401, 959, 996, 1196
HhaI GCGC 2 cut(s) 752, 1297
Hin1II CATG 2 cut(s) 71, 777
Hin6I GCGC 2 cut(s) 750, 1295
HinP1I GCGC 2 cut(s) 750, 1295
HindIII AAGCTT 1 cut(s) 1007
HinfI GANTC 5 cut(s) 53, 248, 268, 916, 1199
HpaII CCGG 6 cut(s) 152, 218, 401, 959, 996, 1196
HphI GGTGA 5 cut(s) 3, 206, 341, 427, 1291
Hpy166II GTNNAC 1 cut(s) 124
Hpy188I TCNGA 6 cut(s) 208, 273, 396, 513, 549, 681
Hpy188III TCNNGA 6 cut(s) 68, 365, 1048, 1084, 1135, 1175
Hpy8I GTNNAC 1 cut(s) 124
HpyCH4III ACNGT 3 cut(s) 74, 181, 1073
HpyCH4IV ACGT 2 cut(s) 8, 855
HpyCH4V TGCA 5 cut(s) 79, 804, 890, 1094, 1340
HpyF10VI GCNNNNNNNGC 2 cut(s) 163, 810
HpyF3I CTNAG 2 cut(s) 213, 465
HpySE526I ACGT 2 cut(s) 8, 855
Hsp92II CATG 2 cut(s) 71, 777
HspAI GCGC 2 cut(s) 750, 1295
Ksp22I TGATCA 1 cut(s) 1131
Kzo9I GATC 6 cut(s) 39, 361, 391, 955, 1018, 1131
Lsp1109I GCAGC 5 cut(s) 153, 156, 734, 1112, 1324
LweI GCATC 2 cut(s) 1306, 1317
MaeI CTAG 2 cut(s) 159, 911
MaeII ACGT 2 cut(s) 8, 855
MaeIII GTNAC 3 cut(s) 9, 92, 757
MalI GATC 6 cut(s) 41, 363, 393, 957, 1020, 1133
MboI GATC 6 cut(s) 39, 361, 391, 955, 1018, 1131
MboII GAAGA 3 cut(s) 243, 277, 1150
MfeI CAATTG 1 cut(s) 951
MflI RGATCY 2 cut(s) 361, 1018
MhlI GDGCHC 1 cut(s) 296
MlsI TGGCCA 2 cut(s) 27, 839
MluCI AATT 2 cut(s) 84, 951
MluNI TGGCCA 2 cut(s) 27, 839
MlyI GAGTC 2 cut(s) 257, 262
MmeI TCCRAC 1 cut(s) 186
Mox20I TGGCCA 2 cut(s) 27, 839
MroXI GAANNNNTTC 1 cut(s) 1203
MscI TGGCCA 2 cut(s) 27, 839
MseI TTAA 3 cut(s) 453, 807, 983
MslI CAYNNNNRTG 2 cut(s) 16, 1313
Msp20I TGGCCA 2 cut(s) 27, 839
MspA1I CMGCKG 2 cut(s) 166, 1229
MspI CCGG 6 cut(s) 152, 218, 401, 959, 996, 1196
MspR9I CCNGG 3 cut(s) 901, 960, 1290
MunI CAATTG 1 cut(s) 951
MvaI CCWGG 2 cut(s) 901, 1290
MvnI CGCG 1 cut(s) 263
MwoI GCNNNNNNNGC 2 cut(s) 163, 810
NciI CCSGG 1 cut(s) 960
NdeII GATC 6 cut(s) 39, 361, 391, 955, 1018, 1131
NlaIII CATG 2 cut(s) 71, 777
NlaIV GGNNCC 7 cut(s) 156, 294, 295, 363, 826, 994, 1075
NmuCI GTSAC 1 cut(s) 9
NspI RCATGY 1 cut(s) 777
OliI CACNNNNGTG 1 cut(s) 16
PagI TCATGA 1 cut(s) 67
PciI ACATGT 1 cut(s) 773
PcsI WCGNNNNNNNCGW 2 cut(s) 14, 260
PdmI GAANNNNTTC 1 cut(s) 1203
PfeI GAWTC 3 cut(s) 53, 916, 1199
PkrI GCNGC 5 cut(s) 168, 171, 749, 1127, 1339
PleI GAGTC 2 cut(s) 256, 262
PpsI GAGTC 2 cut(s) 256, 262
PscI ACATGT 1 cut(s) 773
Psp6I CCWGG 2 cut(s) 899, 1288
PspEI GGTNACC 1 cut(s) 757
PspFI CCCAGC 1 cut(s) 752
PspGI CCWGG 2 cut(s) 899, 1288
PspN4I GGNNCC 7 cut(s) 156, 294, 295, 363, 826, 994, 1075
PspOMI GGGCCC 1 cut(s) 292
PspPI GGNCC 3 cut(s) 292, 293, 825
PstNI CAGNNNCTG 2 cut(s) 371, 1214
PsuI RGATCY 2 cut(s) 361, 1018
PvuII CAGCTG 1 cut(s) 1229
RsaI GTAC 3 cut(s) 123, 849, 1153
RsaNI GTAC 3 cut(s) 122, 848, 1152
RseI CAYNNNNRTG 2 cut(s) 16, 1313
SaqAI TTAA 3 cut(s) 453, 807, 983
SatI GCNGC 5 cut(s) 167, 170, 748, 1126, 1338
Sau3AI GATC 6 cut(s) 39, 361, 391, 955, 1018, 1131
Sau96I GGNCC 3 cut(s) 292, 293, 825
SchI GAGTC 2 cut(s) 257, 262
ScrFI CCNGG 3 cut(s) 901, 960, 1290
SduI GDGCHC 1 cut(s) 296
SfaNI GCATC 2 cut(s) 1306, 1317
SinI GGWCC 1 cut(s) 825
SmiMI CAYNNNNRTG 2 cut(s) 16, 1313
SmlI CTYRAG 1 cut(s) 1046
SmoI CTYRAG 1 cut(s) 1046
Sse9I AATT 2 cut(s) 84, 951
SsiI CCGC 4 cut(s) 164, 261, 779, 1277
SspMI CTAG 2 cut(s) 159, 911
StyD4I CCNGG 3 cut(s) 899, 958, 1288
StyI CCWWGG 2 cut(s) 297, 1028
TaaI ACNGT 3 cut(s) 74, 181, 1073
TaiI ACGT 2 cut(s) 11, 858
TaqI TCGA 2 cut(s) 383, 1331
TaqII GACCGA 1 cut(s) 34
TasI AATT 2 cut(s) 84, 951
TfiI GAWTC 3 cut(s) 53, 916, 1199
Tru1I TTAA 3 cut(s) 453, 807, 983
Tru9I TTAA 3 cut(s) 453, 807, 983
TscAI CASTG 2 cut(s) 445, 1101
TseFI GTSAC 1 cut(s) 9
TseI GCWGC 5 cut(s) 166, 169, 747, 1125, 1337
Tsp45I GTSAC 1 cut(s) 9
TspDTI ATGAA 2 cut(s) 371, 1257
TspGWI ACGGA 2 cut(s) 948, 1281
TspRI CASTG 2 cut(s) 445, 1101
VpaK11BI GGWCC 1 cut(s) 825
XceI RCATGY 1 cut(s) 777
XmnI GAANNNNTTC 1 cut(s) 1203
XspI CTAG 2 cut(s) 159, 911
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.