pycom12g22280

Targeting protein for Xklp2 (TPX2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0005029
Physical Location & Seq
Reverse (-)
23232845 .. 23234865
2021 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 936 bp
ATGGAGAGGAAGGTGGTGGAGAAGAAGATTAACAGTGTCGTGGTGACATCAATTGGTTTTACTCGTGGTAAGGTCCGCGTTGCTCCCAAAGTTTCAGAAGAAAGCATTGAGTCAAAGGATTCCAAGGTGAAGGACCCTGTCAGAGAAAACGAAGTTGCTGTGGAATCTGAGGAGAAGCAAGATGTGCTGGCTGTGAAGAGTACAAATCTTGTTCAAAGTCCAGAGTCTCCGAATGCAGCGTCTCTGAAGAGTTCACAGCCAACTTCTCAGTCGTCACAAGAGGGTGCGGAACATGATAATAAGAAGCATGCTAATGAAGAAGATAATTGGTCTATGACTTCCTCAACTGCTGCATCAAGAACACCTAATTTCAAGGTTACGGTTGGACAAGCTCCCACGTTTAGGATCTATGAGCGTATAGAGAAAAGGAAGGAGTACTACTCGAAGTTAGAGGAAAAGCACCAAGCTATGGAGGCAGAGAGAAGCCAGTGGGAAGCAAGAGCCAAGGAAGAGCAAGAAGCAGCCATCAAGGTGCTGAGGAAGAGCATGGTGGTCAAAGCAAATCCGGTACCCAGTTTCATTTACGAGCCCCCTCCTCCTAAGACCGAACGCAAGAAGGCTCCACTCACTCAGCCCAAGTCCCCAAAATTTAACAGTCTAAGCCGGAGGAAGAGCTATGGTGATGCAATCACCAACTCATCCGCGGAGGAAAAAAAGAAAGGCTGTTCTCGGCCACAACGTCATAGCCTTGGTGCTCAGAAAGAAACTTCCCCCGCGGCTCCCAAAAGTAAGGCTCAGTCTGGAAGGCGAAACAGCCTTGGCGATTGCAAGACGAAAGACCATTCAAAACGCGAAAAGGAAACAACCGAAACATCTCCAAAGACCGCCGAGCAGACATCAGAGTCGAATCACGATGGACTCGAAAGCTGCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

312

Amino Acids

34.7

Weight (kDa)

9.4

Isoelectric Point (pI)

62.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 133 - 209 1.9e-22 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 901
Acc65I GGTACC 1 cut(s) 568
AccB1I GGYRCC 1 cut(s) 568
AccB7I CCANNNNNTGG 1 cut(s) 469
AccII CGCG 4 cut(s) 78, 704, 776, 852
AciI CCGC 7 cut(s) 76, 287, 702, 704, 774, 776, 885
AclWI GGATC 1 cut(s) 413
AcoI YGGCCR 1 cut(s) 731
AcsI RAATTY 1 cut(s) 647
AcuI CTGAAG 1 cut(s) 266
AfaI GTAC 3 cut(s) 202, 437, 570
AfiI CCNNNNNNNGG 2 cut(s) 402, 469
AgsI TTSAA 3 cut(s) 215, 373, 846
AluBI AGCT 4 cut(s) 392, 467, 675, 927
AluI AGCT 4 cut(s) 392, 467, 675, 927
Alw21I GWGCWC 1 cut(s) 757
Alw26I GTCTC 2 cut(s) 231, 246
AlwI GGATC 1 cut(s) 413
AoxI GGCC 1 cut(s) 731
ApeKI GCWGC 4 cut(s) 236, 350, 521, 927
ApoI RAATTY 1 cut(s) 647
ArsI GACNNNNNNTTYG 2 cut(s) 224, 256
Asp718I GGTACC 1 cut(s) 568
AspS9I GGNCC 2 cut(s) 73, 133
AsuHPI GGTGA 4 cut(s) 55, 139, 682, 692
AvaII GGWCC 2 cut(s) 73, 133
BanI GGYRCC 1 cut(s) 568
BanII GRGCYC 1 cut(s) 591
BarI GAAGNNNNNNTAC 2 cut(s) 422, 454
BauI CACGAG 1 cut(s) 63
Bbv12I GWGCWC 1 cut(s) 757
BbvCI CCTCAGC 1 cut(s) 536
BbvI GCAGC 4 cut(s) 248, 337, 533, 914
BccI CCATC 2 cut(s) 533, 908
BcoDI GTCTC 2 cut(s) 231, 246
BisI GCNGC 5 cut(s) 237, 351, 522, 777, 928
BlsI GCNGC 5 cut(s) 238, 352, 523, 778, 929
BmcAI AGTACT 1 cut(s) 437
Bme18I GGWCC 2 cut(s) 73, 133
BmgT120I GGNCC 2 cut(s) 73, 133
BmiI GGNNCC 4 cut(s) 135, 570, 621, 780
BmrI ACTGGG 1 cut(s) 567
BmsI GCATC 2 cut(s) 362, 673
BmuI ACTGGG 1 cut(s) 567
BplI GAGNNNNNCTC 2 cut(s) 425, 457
Bpu10I CCTNAGC 1 cut(s) 536
BsaJI CCNNGG 6 cut(s) 123, 504, 702, 748, 774, 817
BsaWI WCCGGW 1 cut(s) 565
Bsc4I CCNNNNNNNGG 2 cut(s) 402, 469
Bse1I ACTGG 2 cut(s) 487, 573
BseDI CCNNGG 6 cut(s) 123, 504, 702, 748, 774, 817
BseGI GGATG 1 cut(s) 698
BseLI CCNNNNNNNGG 2 cut(s) 402, 469
BseMII CTCAG 6 cut(s) 159, 281, 527, 644, 770, 809
BseNI ACTGG 2 cut(s) 487, 573
BseRI GAGGAG 2 cut(s) 185, 585
BseXI GCAGC 4 cut(s) 248, 337, 533, 914
Bsh1236I CGCG 4 cut(s) 78, 704, 776, 852
BshFI GGCC 1 cut(s) 733
BshNI GGYRCC 1 cut(s) 568
BsiHKAI GWGCWC 1 cut(s) 757
BsiSI CCGG 2 cut(s) 566, 664
BslFI GGGAC 1 cut(s) 625
BslI CCNNNNNNNGG 2 cut(s) 402, 469
BsmAI GTCTC 2 cut(s) 231, 246
BsmBI CGTCTC 1 cut(s) 246
BsmFI GGGAC 1 cut(s) 625
BsmI GAATGC 1 cut(s) 238
BsnI GGCC 1 cut(s) 733
Bsp1286I GDGCHC 2 cut(s) 591, 757
Bsp143I GATC 1 cut(s) 405
BspACI CCGC 7 cut(s) 76, 287, 702, 704, 774, 776, 885
BspANI GGCC 1 cut(s) 733
BspCNI CTCAG 6 cut(s) 160, 280, 528, 643, 769, 808
BspFNI CGCG 4 cut(s) 78, 704, 776, 852
BspLI GGNNCC 4 cut(s) 135, 570, 621, 780
BspPI GGATC 1 cut(s) 413
BspQI GCTCTTC 3 cut(s) 504, 536, 665
BspT107I GGYRCC 1 cut(s) 568
BsrI ACTGG 2 cut(s) 487, 573
BssECI CCNNGG 6 cut(s) 123, 504, 702, 748, 774, 817
BssMI GATC 1 cut(s) 405
BssSI CACGAG 1 cut(s) 63
BssT1I CCWWGG 4 cut(s) 123, 504, 748, 817
Bst2BI CACGAG 1 cut(s) 63
Bst4CI ACNGT 3 cut(s) 35, 382, 656
Bst6I CTCTTC 5 cut(s) 191, 242, 504, 536, 665
BstAPI GCANNNNNTGC 1 cut(s) 184
BstC8I GCNNGC 2 cut(s) 189, 309
BstDEI CTNAG 8 cut(s) 168, 267, 536, 600, 630, 659, 756, 795
BstDSI CCRYGG 2 cut(s) 702, 774
BstF5I GGATG 1 cut(s) 698
BstFNI CGCG 4 cut(s) 78, 704, 776, 852
BstKTI GATC 1 cut(s) 408
BstMAI GTCTC 2 cut(s) 231, 246
BstMBI GATC 1 cut(s) 405
BstMWI GCNNNNNNNGC 2 cut(s) 184, 473
BstNSI RCATGY 1 cut(s) 311
BstUI CGCG 4 cut(s) 78, 704, 776, 852
BstV1I GCAGC 4 cut(s) 248, 337, 533, 914
BstX2I RGATCY 1 cut(s) 405
BstYI RGATCY 1 cut(s) 405
BsuRI GGCC 1 cut(s) 733
BtgI CCRYGG 2 cut(s) 702, 774
BtsCI GGATG 1 cut(s) 698
BtsIMutI CAGTG 2 cut(s) 40, 494
Cac8I GCNNGC 2 cut(s) 189, 309
Cfr13I GGNCC 2 cut(s) 73, 133
Cfr42I CCGCGG 2 cut(s) 705, 777
CseI GACGC 1 cut(s) 228
Csp6I GTAC 3 cut(s) 201, 436, 569
CviAII CATG 3 cut(s) 293, 308, 547
CviQI GTAC 3 cut(s) 201, 436, 569
DdeI CTNAG 8 cut(s) 168, 267, 536, 600, 630, 659, 756, 795
DpnI GATC 1 cut(s) 407
DpnII GATC 1 cut(s) 405
DrdI GACNNNNNNGTC 1 cut(s) 901
DseDI GACNNNNNNGTC 1 cut(s) 901
EaeI YGGCCR 1 cut(s) 731
Eam1104I CTCTTC 5 cut(s) 191, 242, 504, 536, 665
EarI CTCTTC 5 cut(s) 191, 242, 504, 536, 665
Eco130I CCWWGG 4 cut(s) 123, 504, 748, 817
Eco24I GRGCYC 1 cut(s) 591
Eco47I GGWCC 2 cut(s) 73, 133
Eco57I CTGAAG 1 cut(s) 266
EcoO109I RGGNCCY 1 cut(s) 133
EcoT14I CCWWGG 4 cut(s) 123, 504, 748, 817
EcoT38I GRGCYC 1 cut(s) 591
ErhI CCWWGG 4 cut(s) 123, 504, 748, 817
Esp3I CGTCTC 1 cut(s) 246
FaeI CATG 3 cut(s) 296, 311, 550
FaiI YATR 9 cut(s) 294, 309, 335, 411, 419, 470, 548, 678, 744
FaqI GGGAC 1 cut(s) 625
FatI CATG 3 cut(s) 292, 307, 546
FauI CCCGC 1 cut(s) 781
Fnu4HI GCNGC 5 cut(s) 237, 351, 522, 777, 928
FokI GGATG 1 cut(s) 685
FriOI GRGCYC 1 cut(s) 591
Fsp4HI GCNGC 5 cut(s) 237, 351, 522, 777, 928
GluI GCNGC 5 cut(s) 237, 351, 522, 777, 928
HaeIII GGCC 1 cut(s) 733
HapII CCGG 2 cut(s) 566, 664
HgaI GACGC 1 cut(s) 228
Hin1II CATG 3 cut(s) 296, 311, 550
HinfI GANTC 7 cut(s) 110, 119, 164, 224, 902, 907, 918
HpaII CCGG 2 cut(s) 566, 664
HphI GGTGA 4 cut(s) 55, 139, 682, 692
Hpy166II GTNNAC 1 cut(s) 254
Hpy188I TCNGA 7 cut(s) 97, 143, 169, 231, 246, 759, 901
Hpy188III TCNNGA 4 cut(s) 221, 357, 801, 911
Hpy8I GTNNAC 1 cut(s) 254
HpyAV CCTTC 5 cut(s) 4, 124, 424, 610, 798
HpyCH4III ACNGT 3 cut(s) 35, 382, 656
HpyCH4IV ACGT 2 cut(s) 398, 739
HpyCH4V TGCA 4 cut(s) 236, 353, 686, 828
HpyF10VI GCNNNNNNNGC 2 cut(s) 184, 473
HpyF3I CTNAG 8 cut(s) 168, 267, 536, 600, 630, 659, 756, 795
HpySE526I ACGT 2 cut(s) 398, 739
Hsp92II CATG 3 cut(s) 296, 311, 550
KpnI GGTACC 1 cut(s) 572
KspI CCGCGG 2 cut(s) 705, 777
Kzo9I GATC 1 cut(s) 405
LguI GCTCTTC 3 cut(s) 504, 536, 665
LmnI GCTCC 4 cut(s) 88, 397, 625, 784
LpnPI CCDG 8 cut(s) 150, 173, 234, 500, 579, 586, 677, 786
Lsp1109I GCAGC 4 cut(s) 248, 337, 533, 914
LweI GCATC 2 cut(s) 362, 673
MaeII ACGT 2 cut(s) 398, 739
MaeIII GTNAC 3 cut(s) 43, 273, 376
MalI GATC 1 cut(s) 407
MboI GATC 1 cut(s) 405
MfeI CAATTG 1 cut(s) 51
MflI RGATCY 1 cut(s) 405
MhlI GDGCHC 2 cut(s) 591, 757
MluCI AATT 4 cut(s) 51, 325, 367, 647
MlyI GAGTC 4 cut(s) 119, 233, 911, 912
MmeI TCCRAC 1 cut(s) 364
MseI TTAA 2 cut(s) 30, 651
MslI CAYNNNNRTG 2 cut(s) 312, 530
MspA1I CMGCKG 2 cut(s) 704, 776
MspI CCGG 2 cut(s) 566, 664
MunI CAATTG 1 cut(s) 51
Mva1269I GAATGC 1 cut(s) 238
MvnI CGCG 4 cut(s) 78, 704, 776, 852
MwoI GCNNNNNNNGC 2 cut(s) 184, 473
NdeII GATC 1 cut(s) 405
NlaIII CATG 3 cut(s) 296, 311, 550
NlaIV GGNNCC 4 cut(s) 135, 570, 621, 780
NmeAIII GCCGAG 2 cut(s) 709, 913
NmuCI GTSAC 2 cut(s) 43, 273
NspI RCATGY 1 cut(s) 311
PaeI GCATGC 1 cut(s) 311
PciSI GCTCTTC 3 cut(s) 504, 536, 665
PcsI WCGNNNNNNNCGW 2 cut(s) 736, 918
PctI GAATGC 1 cut(s) 238
PfeI GAWTC 3 cut(s) 119, 164, 907
PflFI GACNNNGTC 1 cut(s) 137
PflMI CCANNNNNTGG 1 cut(s) 469
PkrI GCNGC 5 cut(s) 238, 352, 523, 778, 929
PleI GAGTC 4 cut(s) 118, 232, 910, 912
PpsI GAGTC 4 cut(s) 118, 232, 910, 912
PpuMI RGGWCCY 1 cut(s) 133
Psp5II RGGWCCY 1 cut(s) 133
PspN4I GGNNCC 4 cut(s) 135, 570, 621, 780
PspPI GGNCC 2 cut(s) 73, 133
PspPPI RGGWCCY 1 cut(s) 133
PsuI RGATCY 1 cut(s) 405
PsyI GACNNNGTC 1 cut(s) 137
RsaI GTAC 3 cut(s) 202, 437, 570
RsaNI GTAC 3 cut(s) 201, 436, 569
RseI CAYNNNNRTG 2 cut(s) 312, 530
SacII CCGCGG 2 cut(s) 705, 777
SapI GCTCTTC 3 cut(s) 504, 536, 665
SaqAI TTAA 2 cut(s) 30, 651
SatI GCNGC 5 cut(s) 237, 351, 522, 777, 928
Sau3AI GATC 1 cut(s) 405
Sau96I GGNCC 2 cut(s) 73, 133
ScaI AGTACT 1 cut(s) 437
SchI GAGTC 4 cut(s) 119, 233, 911, 912
SduI GDGCHC 2 cut(s) 591, 757
SfaNI GCATC 2 cut(s) 362, 673
Sfr303I CCGCGG 2 cut(s) 705, 777
SgrBI CCGCGG 2 cut(s) 705, 777
SinI GGWCC 2 cut(s) 73, 133
SmiMI CAYNNNNRTG 2 cut(s) 312, 530
SphI GCATGC 1 cut(s) 311
Sse9I AATT 4 cut(s) 51, 325, 367, 647
SsiI CCGC 7 cut(s) 76, 287, 702, 704, 774, 776, 885
StyI CCWWGG 4 cut(s) 123, 504, 748, 817
TaaI ACNGT 3 cut(s) 35, 382, 656
TaiI ACGT 2 cut(s) 401, 742
TaqI TCGA 3 cut(s) 443, 905, 921
TaqII GACCGA 1 cut(s) 620
TasI AATT 4 cut(s) 51, 325, 367, 647
TatI WGTACW 2 cut(s) 200, 435
TauI GCSGC 1 cut(s) 779
TfiI GAWTC 3 cut(s) 119, 164, 907
Tru1I TTAA 2 cut(s) 30, 651
Tru9I TTAA 2 cut(s) 30, 651
TscAI CASTG 2 cut(s) 40, 494
TseFI GTSAC 2 cut(s) 43, 273
TseI GCWGC 4 cut(s) 236, 350, 521, 927
Tsp45I GTSAC 2 cut(s) 43, 273
TspDTI ATGAA 2 cut(s) 330, 568
TspRI CASTG 2 cut(s) 40, 494
Tth111I GACNNNGTC 1 cut(s) 137
Van91I CCANNNNNTGG 1 cut(s) 469
VpaK11BI GGWCC 2 cut(s) 73, 133
XapI RAATTY 1 cut(s) 647
XceI RCATGY 1 cut(s) 311
ZrmI AGTACT 1 cut(s) 437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.