pycom12g23550

Sterol 3-beta-glucosyltransferase UGT80A2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0001484
Physical Location & Seq
Forward (+)
24102945 .. 24108143
5199 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1836 bp
ATGACGGAGTCCTCCCCCGACCACAGCCGGAGCCATTCCGGCAGCTCCAGCGACGGGTCCGTCTATTTCGAAGCCGAAGTCCCTCCCAATCCTAATGACGAAAATGCCACCACTTCCTCAGGGCATTTGGAGGGAGACGTGCAAACAGAGAAAAATTCTGGAAAGTCATCATCCACCGTCCGTACGTCGTCCGGTTCGAGCTCCGGTTCGAGTCAACTCACAGCGAGCGCGCGATTGACTCAGAACCAGGCGTTGGCTGTGATTCTCGCCAAGCTCTTTGATCAAAGAACTCCTTTTAGAAAAAAGCGTAAATATATAAAACGGCTGGCAAAAGTGAAAGATGACGGGACCGTGCAATTTGACGTTCCGGGTGATATTAAACCGCAGTATCTGGATTTCGGAACTGGAGCTGCTCACAGTGAAGCAGCTTCTGGTGGAGAAGATGGAAGCAAGACGGAAACTGAAGTTCGAGATATACCTCCTCTGCAAATTGTAATGCTTATTGTCGGCACACGAGGAGATGTGCAGCCGTTTGTTGCCATTGGAAAGCGATTGCAGGAATATGGCCATAGGGTTAGGCTAGCAACTCATGCAAATTTCAAAGATTTTGTCCTTTCTGCTGGGTTGGAGTTCTATCCTTTAGGTGGATCGATCCAAAGGTTCTTGCTGGTTATGGTCAAGAATAAAGGTTTCTTGCCATCAGGACCTTCGGAAATATCAATTCAGCGACATCAGTTGAAGGAAATAATTTTTTCTTTACTTCCTGCATGCAACGAACCTGATCCTGATTCCAAAATTCCATTTAAAGCAGAAGCAATAATTGCCAATCCACCAGCCTATGGGCACTCTCATGTCGCCGAGGCACTAAAAGTACCACTTCATATATTTTTCACAATGCCATGGACGCCTACGAGTGAATTCCCCCATCCCCTATCTCGTGTCAAGCAGCCAATTGGATATAGACTATCATATCAATTAGTCGATGCATTTATTTGGCTAGGAATTCGAGACATGATTAATGAGTTTAGGAAGAAAATGCTGAAGCTCAGACCTATCACATATTTAAGTGGATACTATAGTTCTCCTCCTGACGTGCCCTATGGGTATATATGGAGTCCTCATCTTGTTCCCAAACCTCAAGATTGGGGACCAAAGGTTGACGTTGTTGGGTTTTGTTTTCTTGACCTTGCTTCAAATTATGAACCCCCTGAGCCACTTGCTAAGTGGCTTGAAGCTGGTGACGCGCCTGTATATATTGGATTTGGTAGCCTTCCTCTTGAAGAACCAGAGAAAATGACTGGTATTATTCTTCAAGCTCTGGAAATAACTGGACAGAGAGGCATTATCAACAGAGGCTGGGGTGGCCTTGGTAATTCGGTAGAACCAAGTGATTCTGTGTACTTAGTGGACAACTGCCCCCATGATTGGTTATTCAAACGATGCTCTGGTGTGGTACATCATGGGGGTGCTGGAACAACTGCTGCTGGTCTGAAAGCTGAGTGTCCGACTACAATTATACCATTCTTCGGGGACCAGCCATTTTGGGGCGAGAGGGTGCATGCCAGAGGTGTAGGTCCTGCACCCATCCCAGTCGAGGAATTCTCGCTTGAAAAATTGGTTGATGCCATACGCTTCATGCTAGATCCAATGGTTAAAGAGCGTGCTGTAGAAATTGCGAAGGCCATGGATAGCGAAGATGGGGTCAAGGGAGCAGTGAAGGCCTTTCACAGGCATTTTCCTTGCAATAAATCTGATGACGACGAACCTGATCAGTCATTGCCAACTAGAAGAAGGTTATTATCTTTCAGGAGATGTTTTGGCTACTCCCCCGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0008194 GO:0016740 GO:0016757 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_transf_28 UDPGT

Protein Analysis

612

Amino Acids

67.27

Weight (kDa)

6.54

Isoelectric Point (pI)

43.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_28 PF03033 164 - 307 1.3e-30 Glycosyltransferase family 28 N-terminal domain
EryCIII-like_C PF06722 464 - 565 1.1e-12 Erythromycin biosynthesis protein CIII-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 59
AccB7I CCANNNNNTGG 2 cut(s) 253, 839
AccII CGCG 3 cut(s) 230, 232, 1244
AciI CCGC 2 cut(s) 383, 1830
AclWI GGATC 4 cut(s) 646, 655, 776, 1637
AcoI YGGCCR 1 cut(s) 565
AcsI RAATTY 6 cut(s) 154, 595, 795, 917, 1002, 1598
AcuI CTGAAG 2 cut(s) 483, 1061
AcyI GRCGYC 1 cut(s) 905
AfaI GTAC 4 cut(s) 184, 873, 1400, 1455
AfiI CCNNNNNNNGG 9 cut(s) 54, 253, 644, 839, 1425, 1526, 1544, 1594, 1728
AgsI TTSAA 8 cut(s) 601, 739, 1194, 1232, 1280, 1313, 1435, 1610
AjiI CACGTC 2 cut(s) 139, 1093
AjnI CCWGG 1 cut(s) 246
AjuI GAANNNNNNNTTGG 2 cut(s) 236, 268
AluBI AGCT 9 cut(s) 45, 201, 274, 410, 428, 1045, 1235, 1316, 1496
AluI AGCT 9 cut(s) 45, 201, 274, 410, 428, 1045, 1235, 1316, 1496
Alw21I GWGCWC 1 cut(s) 203
Alw26I GTCTC 2 cut(s) 129, 1002
AlwI GGATC 4 cut(s) 646, 655, 776, 1637
AlwNI CAGNNNCTG 3 cut(s) 391, 431, 1356
AoxI GGCC 4 cut(s) 565, 1363, 1680, 1719
ApeKI GCWGC 6 cut(s) 42, 410, 425, 526, 946, 1481
ApoI RAATTY 6 cut(s) 154, 595, 795, 917, 1002, 1598
ArsI GACNNNNNNTTYG 6 cut(s) 63, 95, 594, 626, 1686, 1718
AseI ATTAAT 1 cut(s) 1017
AspLEI GCGC 3 cut(s) 230, 232, 1246
AspS9I GGNCC 6 cut(s) 57, 348, 704, 1148, 1531, 1574
AsuC2I CCSGG 1 cut(s) 369
AsuHPI GGTGA 2 cut(s) 383, 1250
AsuII TTCGAA 1 cut(s) 69
AsuNHI GCTAGC 1 cut(s) 580
AvaII GGWCC 6 cut(s) 57, 348, 704, 1148, 1531, 1574
AxyI CCTNAGG 1 cut(s) 118
BaeGI GKGCMC 2 cut(s) 846, 1098
BaeI ACNNNNGTAYC 4 cut(s) 371, 404, 1063, 1096
BalI TGGCCA 1 cut(s) 567
BanII GRGCYC 1 cut(s) 203
BauI CACGAG 2 cut(s) 513, 936
Bbv12I GWGCWC 1 cut(s) 203
BbvI GCAGC 6 cut(s) 54, 397, 437, 538, 958, 1468
BccI CCATC 5 cut(s) 437, 706, 933, 1592, 1691
BceAI ACGGC 2 cut(s) 338, 514
BciT130I CCWGG 1 cut(s) 248
BciVI GTATCC 1 cut(s) 1064
BclI TGATCA 2 cut(s) 280, 1768
BcnI CCSGG 1 cut(s) 369
BcoDI GTCTC 2 cut(s) 129, 1002
BfaI CTAG 4 cut(s) 581, 998, 1640, 1785
BfmI CTRYAG 2 cut(s) 1075, 1665
BfuI GTATCC 1 cut(s) 1064
BglI GCCNNNNNGGC 1 cut(s) 39
BisI GCNGC 6 cut(s) 43, 411, 426, 527, 947, 1482
BlsI GCNGC 6 cut(s) 44, 412, 427, 528, 948, 1483
Bme1390I CCNGG 2 cut(s) 248, 369
Bme18I GGWCC 6 cut(s) 57, 348, 704, 1148, 1531, 1574
BmgBI CACGTC 2 cut(s) 139, 1093
BmgT120I GGNCC 6 cut(s) 57, 348, 704, 1148, 1531, 1574
BmiI GGNNCC 5 cut(s) 32, 58, 349, 1149, 1532
BmrFI CCNGG 2 cut(s) 248, 369
BmrI ACTGGG 1 cut(s) 1583
BmsI GCATC 3 cut(s) 973, 1430, 1612
BmtI GCTAGC 1 cut(s) 584
BmuI ACTGGG 1 cut(s) 1583
BplI GAGNNNNNCTC 2 cut(s) 1586, 1618
BpmI CTGGAG 2 cut(s) 31, 426
Bpu10I CCTNAGC 1 cut(s) 1209
Bpu14I TTCGAA 1 cut(s) 69
BpuEI CTTGAG 1 cut(s) 1122
BpuMI CCSGG 1 cut(s) 369
Bsa29I ATCGAT 1 cut(s) 650
BsaHI GRCGYC 1 cut(s) 905
BsaJI CCNNGG 4 cut(s) 858, 899, 1366, 1683
BsaWI WCCGGW 2 cut(s) 191, 203
Bsc4I CCNNNNNNNGG 9 cut(s) 54, 253, 644, 839, 1425, 1526, 1544, 1594, 1728
Bse1I ACTGG 4 cut(s) 409, 1303, 1333, 1589
Bse21I CCTNAGG 1 cut(s) 118
Bse3DI GCAATG 1 cut(s) 1775
BseBI CCWGG 1 cut(s) 248
BseCI ATCGAT 1 cut(s) 650
BseDI CCNNGG 4 cut(s) 858, 899, 1366, 1683
BseGI GGATG 3 cut(s) 170, 925, 1584
BseLI CCNNNNNNNGG 9 cut(s) 54, 253, 644, 839, 1425, 1526, 1544, 1594, 1728
BseMI GCAATG 1 cut(s) 1775
BseMII CTCAG 5 cut(s) 132, 254, 1060, 1200, 1488
BseNI ACTGG 4 cut(s) 409, 1303, 1333, 1589
BsePI GCGCGC 1 cut(s) 228
BseRI GAGGAG 3 cut(s) 471, 531, 1074
BseSI GKGCMC 2 cut(s) 846, 1098
BseXI GCAGC 6 cut(s) 54, 397, 437, 538, 958, 1468
BseYI CCCAGC 2 cut(s) 620, 1356
BsgI GTGCAG 2 cut(s) 545, 1563
Bsh1236I CGCG 3 cut(s) 230, 232, 1244
BshFI GGCC 4 cut(s) 567, 1365, 1682, 1721
BshVI ATCGAT 1 cut(s) 650
BsiHKAI GWGCWC 1 cut(s) 203
BsiSI CCGG 5 cut(s) 28, 39, 192, 204, 368
BsiWI CGTACG 1 cut(s) 182
BslFI GGGAC 4 cut(s) 65, 361, 1161, 1544
BslI CCNNNNNNNGG 9 cut(s) 54, 253, 644, 839, 1425, 1526, 1544, 1594, 1728
BsmAI GTCTC 2 cut(s) 129, 1002
BsmBI CGTCTC 1 cut(s) 129
BsmFI GGGAC 4 cut(s) 65, 361, 1161, 1544
BsnI GGCC 4 cut(s) 567, 1365, 1682, 1721
Bsp119I TTCGAA 1 cut(s) 69
Bsp1286I GDGCHC 3 cut(s) 203, 846, 1098
Bsp143I GATC 6 cut(s) 280, 647, 651, 781, 1642, 1768
Bsp19I CCATGG 2 cut(s) 899, 1683
BspACI CCGC 2 cut(s) 383, 1830
BspANI GGCC 4 cut(s) 567, 1365, 1682, 1721
BspCNI CTCAG 5 cut(s) 131, 253, 1059, 1201, 1489
BspDI ATCGAT 1 cut(s) 650
BspFNI CGCG 3 cut(s) 230, 232, 1244
BspLI GGNNCC 5 cut(s) 32, 58, 349, 1149, 1532
BspOI GCTAGC 1 cut(s) 584
BspPI GGATC 4 cut(s) 646, 655, 776, 1637
BspT104I TTCGAA 1 cut(s) 69
BsrDI GCAATG 1 cut(s) 1775
BsrI ACTGG 4 cut(s) 409, 1303, 1333, 1589
BssECI CCNNGG 4 cut(s) 858, 899, 1366, 1683
BssHII GCGCGC 1 cut(s) 228
BssMI GATC 6 cut(s) 280, 647, 651, 781, 1642, 1768
BssNI GRCGYC 1 cut(s) 905
BssSI CACGAG 2 cut(s) 513, 936
BssT1I CCWWGG 3 cut(s) 899, 1366, 1683
Bst2BI CACGAG 2 cut(s) 513, 936
Bst2UI CCWGG 1 cut(s) 248
Bst4CI ACNGT 3 cut(s) 178, 352, 419
BstACI GRCGYC 1 cut(s) 905
BstAPI GCANNNNNTGC 2 cut(s) 590, 821
BstBI TTCGAA 1 cut(s) 69
BstC8I GCNNGC 7 cut(s) 226, 230, 327, 582, 769, 1560, 1662
BstDEI CTNAG 7 cut(s) 118, 240, 1046, 1209, 1221, 1402, 1497
BstDSI CCRYGG 2 cut(s) 899, 1683
BstENI CCTNNNNNAGG 1 cut(s) 1726
BstF5I GGATG 3 cut(s) 170, 925, 1584
BstFNI CGCG 3 cut(s) 230, 232, 1244
BstHHI GCGC 3 cut(s) 230, 232, 1246
BstKTI GATC 6 cut(s) 283, 650, 654, 784, 1645, 1771
BstMAI GTCTC 2 cut(s) 129, 1002
BstMBI GATC 6 cut(s) 280, 647, 651, 781, 1642, 1768
BstMWI GCNNNNNNNGC 8 cut(s) 39, 48, 590, 821, 904, 1241, 1362, 1718
BstNI CCWGG 1 cut(s) 248
BstNSI RCATGY 2 cut(s) 771, 1562
BstSCI CCNGG 2 cut(s) 246, 367
BstSFI CTRYAG 2 cut(s) 1075, 1665
BstSLI GKGCMC 2 cut(s) 846, 1098
BstUI CGCG 3 cut(s) 230, 232, 1244
BstV1I GCAGC 6 cut(s) 54, 397, 437, 538, 958, 1468
BstX2I RGATCY 1 cut(s) 1642
BstYI RGATCY 1 cut(s) 1642
Bsu15I ATCGAT 1 cut(s) 650
Bsu36I CCTNAGG 1 cut(s) 118
BsuI GTATCC 1 cut(s) 1064
BsuRI GGCC 4 cut(s) 567, 1365, 1682, 1721
BsuTUI ATCGAT 1 cut(s) 650
BtgI CCRYGG 2 cut(s) 899, 1683
BtrI CACGTC 2 cut(s) 139, 1093
BtsCI GGATG 3 cut(s) 170, 925, 1584
BtsI GCAGTG 1 cut(s) 1719
BtsIMutI CAGTG 2 cut(s) 424, 1719
Cac8I GCNNGC 7 cut(s) 226, 230, 327, 582, 769, 1560, 1662
CaiI CAGNNNCTG 3 cut(s) 391, 431, 1356
CfoI GCGC 3 cut(s) 230, 232, 1246
Cfr13I GGNCC 6 cut(s) 57, 348, 704, 1148, 1531, 1574
ClaI ATCGAT 1 cut(s) 650
CseI GACGC 2 cut(s) 913, 1250
Csp6I GTAC 4 cut(s) 183, 872, 1399, 1454
CviQI GTAC 4 cut(s) 183, 872, 1399, 1454
DdeI CTNAG 7 cut(s) 118, 240, 1046, 1209, 1221, 1402, 1497
DpnI GATC 6 cut(s) 282, 649, 653, 783, 1644, 1770
DpnII GATC 6 cut(s) 280, 647, 651, 781, 1642, 1768
DraI TTTAAA 1 cut(s) 805
DrdI GACNNNNNNGTC 1 cut(s) 59
DseDI GACNNNNNNGTC 1 cut(s) 59
EaeI YGGCCR 1 cut(s) 565
Ecl136II GAGCTC 1 cut(s) 201
Eco130I CCWWGG 3 cut(s) 899, 1366, 1683
Eco147I AGGCCT 1 cut(s) 1721
Eco24I GRGCYC 1 cut(s) 203
Eco47I GGWCC 6 cut(s) 57, 348, 704, 1148, 1531, 1574
Eco53kI GAGCTC 1 cut(s) 201
Eco57I CTGAAG 2 cut(s) 483, 1061
Eco81I CCTNAGG 1 cut(s) 118
EcoICRI GAGCTC 1 cut(s) 201
EcoNI CCTNNNNNAGG 1 cut(s) 1726
EcoO109I RGGNCCY 2 cut(s) 704, 1574
EcoRI GAATTC 3 cut(s) 917, 1002, 1598
EcoRII CCWGG 1 cut(s) 246
EcoT14I CCWWGG 3 cut(s) 899, 1366, 1683
EcoT22I ATGCAT 1 cut(s) 988
EcoT38I GRGCYC 1 cut(s) 203
ErhI CCWWGG 3 cut(s) 899, 1366, 1683
Esp3I CGTCTC 1 cut(s) 129
FalI AAGNNNNNCTT 4 cut(s) 277, 309, 861, 893
FaqI GGGAC 4 cut(s) 65, 361, 1161, 1544
FbaI TGATCA 2 cut(s) 280, 1768
Fnu4HI GCNGC 6 cut(s) 43, 411, 426, 527, 947, 1482
FokI GGATG 3 cut(s) 157, 912, 1571
FriOI GRGCYC 1 cut(s) 203
Fsp4HI GCNGC 6 cut(s) 43, 411, 426, 527, 947, 1482
FspBI CTAG 4 cut(s) 581, 998, 1640, 1785
GlaI GCGC 3 cut(s) 229, 231, 1245
GluI GCNGC 6 cut(s) 43, 411, 426, 527, 947, 1482
GsaI CCCAGC 2 cut(s) 624, 1360
GsuI CTGGAG 2 cut(s) 31, 426
HaeIII GGCC 4 cut(s) 567, 1365, 1682, 1721
HapII CCGG 5 cut(s) 28, 39, 192, 204, 368
HgaI GACGC 2 cut(s) 913, 1250
HhaI GCGC 3 cut(s) 230, 232, 1246
Hin1I GRCGYC 1 cut(s) 905
Hin6I GCGC 3 cut(s) 228, 230, 1244
HinP1I GCGC 3 cut(s) 228, 230, 1244
HincII GTYRAC 2 cut(s) 215, 1159
HindII GTYRAC 2 cut(s) 215, 1159
HinfI GANTC 7 cut(s) 8, 211, 238, 262, 788, 1114, 1391
HpaII CCGG 5 cut(s) 28, 39, 192, 204, 368
HphI GGTGA 2 cut(s) 383, 1250
Hpy166II GTNNAC 4 cut(s) 215, 1159, 1399, 1408
Hpy188I TCNGA 7 cut(s) 243, 401, 712, 1049, 1491, 1506, 1753
Hpy8I GTNNAC 4 cut(s) 215, 1159, 1399, 1408
Hpy99I CGWCG 3 cut(s) 56, 190, 1763
HpyAV CCTTC 6 cut(s) 717, 733, 1280, 1672, 1711, 1785
HpyCH4III ACNGT 3 cut(s) 178, 352, 419
HpyCH4IV ACGT 5 cut(s) 138, 185, 363, 1092, 1161
HpyF10VI GCNNNNNNNGC 8 cut(s) 39, 48, 590, 821, 904, 1241, 1362, 1718
HpyF3I CTNAG 7 cut(s) 118, 240, 1046, 1209, 1221, 1402, 1497
HpySE526I ACGT 5 cut(s) 138, 185, 363, 1092, 1161
Hsp92I GRCGYC 1 cut(s) 905
HspAI GCGC 3 cut(s) 228, 230, 1244
Ksp22I TGATCA 2 cut(s) 280, 1768
Kzo9I GATC 6 cut(s) 280, 647, 651, 781, 1642, 1768
LmnI GCTCC 5 cut(s) 30, 50, 206, 407, 1709
Lsp1109I GCAGC 6 cut(s) 54, 397, 437, 538, 958, 1468
LweI GCATC 3 cut(s) 973, 1430, 1612
MaeI CTAG 4 cut(s) 581, 998, 1640, 1785
MaeII ACGT 5 cut(s) 138, 185, 363, 1092, 1161
MaeIII GTNAC 1 cut(s) 1238
MalI GATC 6 cut(s) 282, 649, 653, 783, 1644, 1770
MboI GATC 6 cut(s) 280, 647, 651, 781, 1642, 1768
MboII GAAGA 7 cut(s) 452, 1042, 1292, 1301, 1516, 1706, 1800
MfeI CAATTG 1 cut(s) 951
MflI RGATCY 1 cut(s) 1642
MhlI GDGCHC 3 cut(s) 203, 846, 1098
MlsI TGGCCA 1 cut(s) 567
MluNI TGGCCA 1 cut(s) 567
MlyI GAGTC 4 cut(s) 17, 220, 232, 1123
MmeI TCCRAC 2 cut(s) 606, 1529
Mox20I TGGCCA 1 cut(s) 567
Mph1103I ATGCAT 1 cut(s) 988
MscI TGGCCA 1 cut(s) 567
MseI TTAA 5 cut(s) 378, 804, 1017, 1064, 1653
MslI CAYNNNNRTG 2 cut(s) 849, 1464
Msp20I TGGCCA 1 cut(s) 567
MspI CCGG 5 cut(s) 28, 39, 192, 204, 368
MspR9I CCNGG 2 cut(s) 248, 369
MunI CAATTG 1 cut(s) 951
MvaI CCWGG 1 cut(s) 248
MvnI CGCG 3 cut(s) 230, 232, 1244
MwoI GCNNNNNNNGC 8 cut(s) 39, 48, 590, 821, 904, 1241, 1362, 1718
NciI CCSGG 1 cut(s) 369
NcoI CCATGG 2 cut(s) 899, 1683
NdeII GATC 6 cut(s) 280, 647, 651, 781, 1642, 1768
NheI GCTAGC 1 cut(s) 580
NlaIV GGNNCC 5 cut(s) 32, 58, 349, 1149, 1532
NmeAIII GCCGAG 1 cut(s) 883
NmuCI GTSAC 1 cut(s) 1238
NsiI ATGCAT 1 cut(s) 988
NspI RCATGY 2 cut(s) 771, 1562
NspV TTCGAA 1 cut(s) 69
PaeI GCATGC 2 cut(s) 771, 1562
PauI GCGCGC 1 cut(s) 228
PceI AGGCCT 1 cut(s) 1721
PcsI WCGNNNNNNNCGW 1 cut(s) 194
PfeI GAWTC 3 cut(s) 262, 788, 1391
Pfl23II CGTACG 1 cut(s) 182
PflFI GACNNNGTC 1 cut(s) 7
PflMI CCANNNNNTGG 2 cut(s) 253, 839
PkrI GCNGC 6 cut(s) 44, 412, 427, 528, 948, 1483
PleI GAGTC 4 cut(s) 16, 219, 232, 1122
PpsI GAGTC 4 cut(s) 16, 219, 232, 1122
PpuMI RGGWCCY 2 cut(s) 704, 1574
PshBI ATTAAT 1 cut(s) 1017
Psp124BI GAGCTC 1 cut(s) 203
Psp5II RGGWCCY 2 cut(s) 704, 1574
Psp6I CCWGG 1 cut(s) 246
PspFI CCCAGC 2 cut(s) 620, 1356
PspGI CCWGG 1 cut(s) 246
PspLI CGTACG 1 cut(s) 182
PspN4I GGNNCC 5 cut(s) 32, 58, 349, 1149, 1532
PspPI GGNCC 6 cut(s) 57, 348, 704, 1148, 1531, 1574
PspPPI RGGWCCY 2 cut(s) 704, 1574
PstNI CAGNNNCTG 3 cut(s) 391, 431, 1356
PsuI RGATCY 1 cut(s) 1642
PsyI GACNNNGTC 1 cut(s) 7
PteI GCGCGC 1 cut(s) 228
RsaI GTAC 4 cut(s) 184, 873, 1400, 1455
RsaNI GTAC 4 cut(s) 183, 872, 1399, 1454
RseI CAYNNNNRTG 2 cut(s) 849, 1464
SacI GAGCTC 1 cut(s) 203
SaqAI TTAA 5 cut(s) 378, 804, 1017, 1064, 1653
SatI GCNGC 6 cut(s) 43, 411, 426, 527, 947, 1482
Sau3AI GATC 6 cut(s) 280, 647, 651, 781, 1642, 1768
Sau96I GGNCC 6 cut(s) 57, 348, 704, 1148, 1531, 1574
SchI GAGTC 4 cut(s) 17, 220, 232, 1123
ScrFI CCNGG 2 cut(s) 248, 369
SduI GDGCHC 3 cut(s) 203, 846, 1098
SfaNI GCATC 3 cut(s) 973, 1430, 1612
SfcI CTRYAG 2 cut(s) 1075, 1665
SfuI TTCGAA 1 cut(s) 69
SinI GGWCC 6 cut(s) 57, 348, 704, 1148, 1531, 1574
SmiMI CAYNNNNRTG 2 cut(s) 849, 1464
SmlI CTYRAG 1 cut(s) 1137
SmoI CTYRAG 1 cut(s) 1137
SphI GCATGC 2 cut(s) 771, 1562
SseBI AGGCCT 1 cut(s) 1721
SsiI CCGC 2 cut(s) 383, 1830
SspMI CTAG 4 cut(s) 581, 998, 1640, 1785
SstI GAGCTC 1 cut(s) 203
StuI AGGCCT 1 cut(s) 1721
StyD4I CCNGG 2 cut(s) 246, 367
StyI CCWWGG 3 cut(s) 899, 1366, 1683
TaaI ACNGT 3 cut(s) 178, 352, 419
TaiI ACGT 5 cut(s) 141, 188, 366, 1095, 1164
TaqI TCGA 8 cut(s) 69, 197, 209, 469, 650, 981, 1006, 1593
TatI WGTACW 1 cut(s) 1398
TfiI GAWTC 3 cut(s) 262, 788, 1391
Tru1I TTAA 5 cut(s) 378, 804, 1017, 1064, 1653
Tru9I TTAA 5 cut(s) 378, 804, 1017, 1064, 1653
TscAI CASTG 2 cut(s) 424, 1719
TseFI GTSAC 1 cut(s) 1238
TseI GCWGC 6 cut(s) 42, 410, 425, 526, 946, 1481
Tsp45I GTSAC 1 cut(s) 1238
TspDTI ATGAA 3 cut(s) 869, 1215, 1624
TspGWI ACGGA 4 cut(s) 20, 49, 170, 470
TspRI CASTG 2 cut(s) 424, 1719
Tth111I GACNNNGTC 1 cut(s) 7
Van91I CCANNNNNTGG 2 cut(s) 253, 839
VpaK11BI GGWCC 6 cut(s) 57, 348, 704, 1148, 1531, 1574
VspI ATTAAT 1 cut(s) 1017
XagI CCTNNNNNAGG 1 cut(s) 1726
XapI RAATTY 6 cut(s) 154, 595, 795, 917, 1002, 1598
XceI RCATGY 2 cut(s) 771, 1562
XcmI CCANNNNNNNNNTGG 1 cut(s) 1221
XspI CTAG 4 cut(s) 581, 998, 1640, 1785
Zsp2I ATGCAT 1 cut(s) 988
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.