pycom13g01630

S-adenosylmethionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0008919
Physical Location & Seq
Forward (+)
1077632 .. 1080095
2464 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1089 bp
ATGAACGGTGGAAATGGCACTTACAGCTACTCCAAGAACTCCTCCTACCAGAAAGAATCCGCTGATCTTGAGAAGGGAAAAATCATTGAGGAAATTGAGCAGAAGCTTGATATGAAAAAACTCTCATCTTTTTCAAACACCATTTGTCTAGCAGACTTGGGATGTTCCACTGGACCAAACACTTTCATGATAATTCAAGATATACTAGAAGCTATGCAACGCAAACACCGATCCCAGTTATCCCAATCATGTCCCAAAGCTGATCAACAATACTCTGATGATCATCAAATACCTGAATTTCAAGTCTTCTTTAACGACCTAAAGGCGAATGACTTCAATACCCTCTTCACCTCTCTCCCACAAGACAGGAAATACTTTGCAGCCGGCGTGCCCGGTTCTTTCCATCACCGTTTGTTTCCCAAGTCGTCTATCCACTTTGTACATACCTCATCTAGCCTTCATTGGATCTCCAAGTCGCCTGAAGTATTGCAAAACAAGGACTCTCCGGCATGGAACAAGGGCAGGATTCACTACACAAGTGCCCCGGATGAAGTGGTTGATGCTTATGCGTCGCAGTTTGCCGAGGATATGAAGAACTTTTTGAATGTTAGGGCCAAAGAGCTTGTGCATGGAGGAATGATGGTAATGATCTTGTCTGGAATCCCCAAAGGGATGCCTTATTCTGAAATCCCAGCGGGTATGTTGTACAATTGTATGTCGTCTAGCCTCATGGATATGGCAAAAGAGGGAATAATTGAAGAAAGTGAAGTGGATTCCTTCAACTTGCCATACTATGCAGCCTCGTTAGAGGAGATGGAGGAGATAGTAAAGAAAAACGGGTGTTTTAAGATAGAGAAAATGGAGTCGTCAAACCCAGCAGCATGGCTGAAAGGTCGTCCAGTTGACATACCAACTTTGGTGAAACATGTAAGGGCTGCAGTGGAGGGGATGTTTGCCAAACACTTTGGAATTGAGGTCATGGATGAAATGTTCGGAAGAGTGACCGATAAACTTCTAGACATTTCCGACCTCGTAAACTCACAACGCGACGAGAAAAGTCAGTTGCTTGCTGTTCTCAAACGCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

363

Amino Acids

40.8

Weight (kDa)

5.91

Isoelectric Point (pI)

48.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 39 - 361 2.5e-107 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 1047
AciI CCGC 2 cut(s) 60, 695
AclWI GGATC 2 cut(s) 225, 473
AcsI RAATTY 1 cut(s) 296
AcuI CTGAAG 1 cut(s) 501
AfaI GTAC 2 cut(s) 441, 707
AflIII ACRYGT 1 cut(s) 925
AgsI TTSAA 7 cut(s) 135, 197, 302, 337, 604, 758, 781
AloI GAACNNNNNNTCC 2 cut(s) 974, 1006
AluBI AGCT 5 cut(s) 27, 106, 212, 260, 622
AluI AGCT 5 cut(s) 27, 106, 212, 260, 622
AlwI GGATC 2 cut(s) 225, 473
AoxI GGCC 1 cut(s) 612
ApeKI GCWGC 4 cut(s) 380, 797, 878, 935
ApoI RAATTY 1 cut(s) 296
Asp700I GAANNNNTTC 1 cut(s) 332
AspS9I GGNCC 2 cut(s) 173, 612
AsuC2I CCSGG 2 cut(s) 393, 545
AsuHPI GGTGA 3 cut(s) 340, 398, 931
AvaII GGWCC 1 cut(s) 173
BaeGI GKGCMC 2 cut(s) 393, 544
BbsI GAAGAC 1 cut(s) 298
BbvI GCAGC 4 cut(s) 392, 809, 890, 922
BccI CCATC 3 cut(s) 411, 634, 808
BclI TGATCA 2 cut(s) 262, 280
BcnI CCSGG 2 cut(s) 393, 545
BfaI CTAG 5 cut(s) 149, 206, 453, 723, 1016
BfmI CTRYAG 1 cut(s) 936
BisI GCNGC 4 cut(s) 381, 798, 879, 936
BlsI GCNGC 4 cut(s) 382, 799, 880, 937
Bme1390I CCNGG 2 cut(s) 393, 545
Bme18I GGWCC 1 cut(s) 173
BmgT120I GGNCC 2 cut(s) 173, 612
BmrFI CCNGG 2 cut(s) 393, 545
BmrI ACTGGG 1 cut(s) 229
BmsI GCATC 2 cut(s) 550, 663
BmuI ACTGGG 1 cut(s) 229
BpiI GAAGAC 1 cut(s) 298
BpuEI CTTGAG 1 cut(s) 89
BpuMI CCSGG 2 cut(s) 393, 545
BsaBI GATNNNNATC 1 cut(s) 282
BsaJI CCNNGG 2 cut(s) 543, 582
BsaXI ACNNNNNCTCC 4 cut(s) 14, 44, 935, 965
Bse118I RCCGGY 1 cut(s) 383
Bse1I ACTGG 3 cut(s) 175, 235, 899
Bse8I GATNNNNATC 1 cut(s) 282
BseDI CCNNGG 2 cut(s) 543, 582
BseGI GGATG 5 cut(s) 167, 553, 678, 954, 988
BseJI GATNNNNATC 1 cut(s) 282
BseNI ACTGG 3 cut(s) 175, 235, 899
BseRI GAGGAG 3 cut(s) 31, 824, 833
BseSI GKGCMC 2 cut(s) 393, 544
BseXI GCAGC 4 cut(s) 392, 809, 890, 922
BseYI CCCAGC 2 cut(s) 691, 874
Bsh1236I CGCG 1 cut(s) 1047
BshFI GGCC 1 cut(s) 614
BsiSI CCGG 4 cut(s) 384, 393, 506, 545
BslFI GGGAC 1 cut(s) 237
BsmFI GGGAC 1 cut(s) 237
BsnI GGCC 1 cut(s) 614
Bsp1286I GDGCHC 2 cut(s) 393, 544
Bsp1407I TGTACA 2 cut(s) 439, 705
Bsp143I GATC 6 cut(s) 64, 230, 262, 280, 465, 648
BspACI CCGC 2 cut(s) 60, 695
BspANI GGCC 1 cut(s) 614
BspFNI CGCG 1 cut(s) 1047
BspHI TCATGA 1 cut(s) 186
BspMAI CTGCAG 1 cut(s) 940
BspPI GGATC 2 cut(s) 225, 473
BsrFI RCCGGY 1 cut(s) 383
BsrGI TGTACA 2 cut(s) 439, 705
BsrI ACTGG 3 cut(s) 175, 235, 899
BssAI RCCGGY 1 cut(s) 383
BssECI CCNNGG 2 cut(s) 543, 582
BssMI GATC 6 cut(s) 64, 230, 262, 280, 465, 648
Bst4CI ACNGT 2 cut(s) 8, 410
Bst6I CTCTTC 2 cut(s) 350, 991
BstAUI TGTACA 2 cut(s) 439, 705
BstC8I GCNNGC 3 cut(s) 385, 389, 1068
BstF5I GGATG 5 cut(s) 167, 553, 678, 954, 988
BstFNI CGCG 1 cut(s) 1047
BstKTI GATC 6 cut(s) 67, 233, 265, 283, 468, 651
BstMBI GATC 6 cut(s) 64, 230, 262, 280, 465, 648
BstMWI GCNNNNNNNGC 1 cut(s) 24
BstNSI RCATGY 1 cut(s) 929
BstSCI CCNGG 2 cut(s) 391, 543
BstSFI CTRYAG 1 cut(s) 936
BstSLI GKGCMC 2 cut(s) 393, 544
BstUI CGCG 1 cut(s) 1047
BstV1I GCAGC 4 cut(s) 392, 809, 890, 922
BstV2I GAAGAC 1 cut(s) 298
BstX2I RGATCY 1 cut(s) 465
BstXI CCANNNNNNTGG 1 cut(s) 882
BstYI RGATCY 1 cut(s) 465
BsuRI GGCC 1 cut(s) 614
BtsCI GGATG 5 cut(s) 167, 553, 678, 954, 988
BtsI GCAGTG 1 cut(s) 945
BtsIMutI CAGTG 2 cut(s) 168, 945
Cac8I GCNNGC 3 cut(s) 385, 389, 1068
CciI TCATGA 1 cut(s) 186
Cfr10I RCCGGY 1 cut(s) 383
Cfr13I GGNCC 2 cut(s) 173, 612
CseI GACGC 1 cut(s) 558
Csp6I GTAC 2 cut(s) 440, 706
CviAII CATG 8 cut(s) 187, 249, 510, 629, 730, 882, 926, 979
CviQI GTAC 2 cut(s) 440, 706
DpnI GATC 6 cut(s) 66, 232, 264, 282, 467, 650
DpnII GATC 6 cut(s) 64, 230, 262, 280, 465, 648
Eam1104I CTCTTC 2 cut(s) 350, 991
EarI CTCTTC 2 cut(s) 350, 991
Eco47I GGWCC 1 cut(s) 173
Eco57I CTGAAG 1 cut(s) 501
FaeI CATG 8 cut(s) 190, 252, 513, 632, 733, 885, 929, 982
FaqI GGGAC 1 cut(s) 237
FatI CATG 8 cut(s) 186, 248, 509, 628, 729, 881, 925, 978
FauI CCCGC 1 cut(s) 688
FbaI TGATCA 2 cut(s) 262, 280
Fnu4HI GCNGC 4 cut(s) 381, 798, 879, 936
FokI GGATG 5 cut(s) 174, 560, 685, 961, 995
Fsp4HI GCNGC 4 cut(s) 381, 798, 879, 936
FspBI CTAG 5 cut(s) 149, 206, 453, 723, 1016
GluI GCNGC 4 cut(s) 381, 798, 879, 936
GsaI CCCAGC 2 cut(s) 695, 878
HaeIII GGCC 1 cut(s) 614
HapII CCGG 4 cut(s) 384, 393, 506, 545
HgaI GACGC 1 cut(s) 558
Hin1II CATG 8 cut(s) 190, 252, 513, 632, 733, 885, 929, 982
HincII GTYRAC 1 cut(s) 904
HindII GTYRAC 1 cut(s) 904
HindIII AAGCTT 1 cut(s) 104
HinfI GANTC 6 cut(s) 56, 500, 526, 660, 773, 863
HpaII CCGG 4 cut(s) 384, 393, 506, 545
HphI GGTGA 3 cut(s) 340, 398, 931
Hpy166II GTNNAC 2 cut(s) 904, 1036
Hpy188I TCNGA 4 cut(s) 277, 685, 995, 1027
Hpy188III TCNNGA 5 cut(s) 68, 187, 197, 657, 1016
Hpy8I GTNNAC 2 cut(s) 904, 1036
Hpy99I CGWCG 2 cut(s) 574, 1052
HpyAV CCTTC 3 cut(s) 67, 467, 787
HpyCH4III ACNGT 2 cut(s) 8, 410
HpyCH4V TGCA 6 cut(s) 217, 380, 490, 628, 797, 938
HpyF10VI GCNNNNNNNGC 1 cut(s) 24
Hsp92II CATG 8 cut(s) 190, 252, 513, 632, 733, 885, 929, 982
KroI GCCGGC 1 cut(s) 383
KroNI GCCGGC 1 cut(s) 385
Ksp22I TGATCA 2 cut(s) 262, 280
Kzo9I GATC 6 cut(s) 64, 230, 262, 280, 465, 648
Lsp1109I GCAGC 4 cut(s) 392, 809, 890, 922
LweI GCATC 2 cut(s) 550, 663
MaeI CTAG 5 cut(s) 149, 206, 453, 723, 1016
MaeIII GTNAC 1 cut(s) 1000
MalI GATC 6 cut(s) 66, 232, 264, 282, 467, 650
MboI GATC 6 cut(s) 64, 230, 262, 280, 465, 648
MboII GAAGA 5 cut(s) 298, 337, 604, 770, 1008
MfeI CAATTG 1 cut(s) 709
MflI RGATCY 1 cut(s) 465
MhlI GDGCHC 2 cut(s) 393, 544
MluCI AATT 6 cut(s) 93, 192, 296, 709, 753, 969
MlyI GAGTC 2 cut(s) 494, 872
MmeI TCCRAC 1 cut(s) 1050
MroNI GCCGGC 1 cut(s) 383
MroXI GAANNNNTTC 1 cut(s) 332
MseI TTAA 2 cut(s) 312, 846
MslI CAYNNNNRTG 2 cut(s) 185, 734
MspA1I CMGCKG 2 cut(s) 62, 695
MspI CCGG 4 cut(s) 384, 393, 506, 545
MspR9I CCNGG 2 cut(s) 393, 545
MunI CAATTG 1 cut(s) 709
MvnI CGCG 1 cut(s) 1047
MwoI GCNNNNNNNGC 1 cut(s) 24
NaeI GCCGGC 1 cut(s) 385
NciI CCSGG 2 cut(s) 393, 545
NdeII GATC 6 cut(s) 64, 230, 262, 280, 465, 648
NgoMIV GCCGGC 1 cut(s) 383
NlaIII CATG 8 cut(s) 190, 252, 513, 632, 733, 885, 929, 982
NmeAIII GCCGAG 1 cut(s) 607
NmuCI GTSAC 1 cut(s) 1000
NspI RCATGY 1 cut(s) 929
PagI TCATGA 1 cut(s) 186
PciI ACATGT 1 cut(s) 925
PcsI WCGNNNNNNNCGW 1 cut(s) 226
PdiI GCCGGC 1 cut(s) 385
PdmI GAANNNNTTC 1 cut(s) 332
PfeI GAWTC 4 cut(s) 56, 526, 660, 773
PkrI GCNGC 4 cut(s) 382, 799, 880, 937
PleI GAGTC 2 cut(s) 494, 871
PpsI GAGTC 2 cut(s) 494, 871
PscI ACATGT 1 cut(s) 925
PspFI CCCAGC 2 cut(s) 691, 874
PspPI GGNCC 2 cut(s) 173, 612
PsrI GAACNNNNNNTAC 2 cut(s) 29, 61
PstI CTGCAG 1 cut(s) 940
PsuI RGATCY 1 cut(s) 465
RsaI GTAC 2 cut(s) 441, 707
RsaNI GTAC 2 cut(s) 440, 706
RseI CAYNNNNRTG 2 cut(s) 185, 734
SaqAI TTAA 2 cut(s) 312, 846
SatI GCNGC 4 cut(s) 381, 798, 879, 936
Sau3AI GATC 6 cut(s) 64, 230, 262, 280, 465, 648
Sau96I GGNCC 2 cut(s) 173, 612
SchI GAGTC 2 cut(s) 494, 872
ScrFI CCNGG 2 cut(s) 393, 545
SduI GDGCHC 2 cut(s) 393, 544
SfaNI GCATC 2 cut(s) 550, 663
SfcI CTRYAG 1 cut(s) 936
SinI GGWCC 1 cut(s) 173
SmiMI CAYNNNNRTG 2 cut(s) 185, 734
SmlI CTYRAG 1 cut(s) 68
SmoI CTYRAG 1 cut(s) 68
Sse9I AATT 6 cut(s) 93, 192, 296, 709, 753, 969
SsiI CCGC 2 cut(s) 60, 695
SspMI CTAG 5 cut(s) 149, 206, 453, 723, 1016
StyD4I CCNGG 2 cut(s) 391, 543
TaaI ACNGT 2 cut(s) 8, 410
TaqII GACCGA 1 cut(s) 1019
TasI AATT 6 cut(s) 93, 192, 296, 709, 753, 969
TatI WGTACW 2 cut(s) 439, 705
TfiI GAWTC 4 cut(s) 56, 526, 660, 773
Tru1I TTAA 2 cut(s) 312, 846
Tru9I TTAA 2 cut(s) 312, 846
TscAI CASTG 2 cut(s) 175, 945
TseFI GTSAC 1 cut(s) 1000
TseI GCWGC 4 cut(s) 380, 797, 878, 935
Tsp45I GTSAC 1 cut(s) 1000
TspDTI ATGAA 7 cut(s) 17, 128, 175, 449, 564, 605, 999
TspRI CASTG 2 cut(s) 175, 945
VpaK11BI GGWCC 1 cut(s) 173
XapI RAATTY 1 cut(s) 296
XbaI TCTAGA 1 cut(s) 1015
XceI RCATGY 1 cut(s) 929
XmnI GAANNNNTTC 1 cut(s) 332
XspI CTAG 5 cut(s) 149, 206, 453, 723, 1016
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.