pycom13g07270

Cofactor assembly of complex C subunit B, CCB2/CCB4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0007215
Physical Location & Seq
Forward (+)
4803481 .. 4805513
2033 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1029 bp
ATGGTCCAAGTCCTAAAGTCAACAAAAGTCACAAATCTATTTGTCCTTCATGCCTACAAAATTCAGCTAAACGACAGGCCGTTTCCAATCCAAAGCAAAATATCAAATATCAGCGCCACGTCACCTATTCCACAGATTTCGATGGAAGCGGGAAGCCTTGTCCGCAGTCCAATTCCATGGACTCCAAAACGCCCTTTCCGACGTCGTCTCGTCCCCGCTTTCCCGCGCAGCATCAGGGCGTCGAATTCAGACACTCGTTACAGAGGCCCAAAGCCCAAGAGAAATTGGGTCGCCGATTGGGTCTCCAACAACGACGACGCCGCTCGTAGCTTGCCGATATACGCCGGCGGCGCCTCTCTCATGGCCGTCCTCTTCAACCGCACCGTTTCGGACATCGCTCTCGTCGCTGACGCCAGTAGTTCACAGTCCAGAGCTGATTTATTGACGTTGGGATTGGCCGTGACCAACATACTAGCAGGTTTGGTGTGGCTGTCGATCAGGCCAAAATCTATATCTAAGGTGAGTCCGGAAGGGGTCGAATGCGAGCAGATGCATCCGAATCTTCCCAAGACTCTACTTTCTGAGTTGTTGTGGGCATGGGAATCTCTCTCCAGCGTGACATGCTGCCGGTCTCTAGTTATAGTTTATGATGGCAGATGTATCCTTCAGATTGGTTTTGCAGCCGAATTTTCCAATGGTGATGGAAAGGCTGTGGATGTGGATGCTGATAAACTGATGCAAGGATCACTCTATCGAGGGGTTATGAAATCAGGAGTTCAGAGCTATTTGGCCAACCTATATCTTTATCCTGGGAAATCCGAGCTACCATTTCTGCCTTCAAATACGCAGGCAGTAATATTGCAGCCACTTGGAGACAAAGGGATTGCTGTAATTGGTGGTGACACAGTAAGAGGATTCACATCGTCTGATCAGGCATGGATTTCATTAATCGGAGAGAAGCTCGATTCTACGCTATCAAAATACGTGGAAAACCTCCCATTGGCTGTACAAGATCCGGTTCTGAAGTAA

Protein Analysis

343

Amino Acids

37.33

Weight (kDa)

9.37

Isoelectric Point (pI)

44.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCB2_CCB4 PF11152 111 - 321 4.4e-59 Cofactor assembly of complex C subunit B, CCB2/CCB4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 205
Acc36I ACCTGC 1 cut(s) 467
AccB1I GGYRCC 1 cut(s) 350
AccBSI CCGCTC 1 cut(s) 323
AccII CGCG 1 cut(s) 226
AccIII TCCGGA 1 cut(s) 526
AciI CCGC 7 cut(s) 149, 163, 216, 224, 321, 348, 379
AclWI GGATC 2 cut(s) 751, 1007
AcoI YGGCCR 3 cut(s) 363, 456, 789
AcsI RAATTY 3 cut(s) 60, 244, 686
AcuI CTGAAG 1 cut(s) 650
AcyI GRCGYC 5 cut(s) 202, 239, 318, 351, 411
AfaI GTAC 1 cut(s) 1008
AfiI CCNNNNNNNGG 1 cut(s) 1000
AgsI TTSAA 2 cut(s) 376, 840
AjiI CACGTC 1 cut(s) 120
AjnI CCWGG 1 cut(s) 808
AluBI AGCT 6 cut(s) 67, 330, 434, 783, 823, 961
AluI AGCT 6 cut(s) 67, 330, 434, 783, 823, 961
Alw26I GTCTC 4 cut(s) 212, 307, 636, 867
AlwI GGATC 2 cut(s) 751, 1007
Aor13HI TCCGGA 1 cut(s) 526
AoxI GGCC 6 cut(s) 77, 265, 363, 456, 500, 789
ApeKI GCWGC 4 cut(s) 228, 624, 680, 862
ApoI RAATTY 3 cut(s) 60, 244, 686
AseI ATTAAT 1 cut(s) 947
AspLEI GCGC 3 cut(s) 116, 228, 353
AspS9I GGNCC 2 cut(s) 4, 266
AsuHPI GGTGA 4 cut(s) 114, 532, 710, 911
AvaII GGWCC 1 cut(s) 4
BalI TGGCCA 1 cut(s) 791
BanI GGYRCC 1 cut(s) 350
BbvI GCAGC 4 cut(s) 240, 611, 692, 874
BccI CCATC 3 cut(s) 136, 644, 695
BceAI ACGGC 3 cut(s) 64, 350, 443
BciT130I CCWGG 1 cut(s) 810
BciVI GTATCC 1 cut(s) 671
BclI TGATCA 1 cut(s) 928
BcoDI GTCTC 4 cut(s) 212, 307, 636, 867
BfaI CTAG 2 cut(s) 473, 635
BfoI RGCGCY 2 cut(s) 117, 354
BfuAI ACCTGC 1 cut(s) 467
BfuI GTATCC 1 cut(s) 671
BisI GCNGC 6 cut(s) 229, 321, 349, 625, 681, 863
BlsI GCNGC 6 cut(s) 230, 322, 350, 626, 682, 864
Bme1390I CCNGG 1 cut(s) 810
Bme18I GGWCC 1 cut(s) 4
BmgBI CACGTC 1 cut(s) 120
BmgT120I GGNCC 2 cut(s) 4, 266
BmiI GGNNCC 1 cut(s) 352
BmrFI CCNGG 1 cut(s) 810
BmsI GCATC 5 cut(s) 240, 540, 562, 712, 726
BplI GAGNNNNNCTC 2 cut(s) 945, 977
BpmI CTGGAG 1 cut(s) 595
BsaAI YACGTR 1 cut(s) 985
BsaBI GATNNNNATC 1 cut(s) 919
BsaHI GRCGYC 5 cut(s) 202, 239, 318, 351, 411
BsaI GGTCTC 2 cut(s) 307, 636
BsaJI CCNNGG 2 cut(s) 176, 809
BsaWI WCCGGW 2 cut(s) 526, 1015
Bsc4I CCNNNNNNNGG 1 cut(s) 1000
Bse118I RCCGGY 2 cut(s) 344, 627
Bse1I ACTGG 1 cut(s) 414
Bse8I GATNNNNATC 1 cut(s) 919
BseAI TCCGGA 1 cut(s) 526
BseBI CCWGG 1 cut(s) 810
BseDI CCNNGG 2 cut(s) 176, 809
BseGI GGATG 3 cut(s) 553, 721, 727
BseJI GATNNNNATC 1 cut(s) 919
BseLI CCNNNNNNNGG 1 cut(s) 1000
BseMII CTCAG 1 cut(s) 573
BseNI ACTGG 1 cut(s) 414
BseXI GCAGC 4 cut(s) 240, 611, 692, 874
Bsh1236I CGCG 1 cut(s) 226
BshFI GGCC 6 cut(s) 79, 267, 365, 458, 502, 791
BshNI GGYRCC 1 cut(s) 350
BsiSI CCGG 4 cut(s) 345, 527, 628, 1016
BslFI GGGAC 1 cut(s) 197
BslI CCNNNNNNNGG 1 cut(s) 1000
BsmAI GTCTC 4 cut(s) 212, 307, 636, 867
BsmBI CGTCTC 1 cut(s) 212
BsmFI GGGAC 1 cut(s) 197
BsmI GAATGC 1 cut(s) 545
BsnI GGCC 6 cut(s) 79, 267, 365, 458, 502, 791
Bso31I GGTCTC 2 cut(s) 307, 636
Bsp13I TCCGGA 1 cut(s) 526
Bsp1407I TGTACA 1 cut(s) 1006
Bsp143I GATC 4 cut(s) 495, 743, 928, 1012
Bsp19I CCATGG 1 cut(s) 176
BspACI CCGC 7 cut(s) 149, 163, 216, 224, 321, 348, 379
BspANI GGCC 6 cut(s) 79, 267, 365, 458, 502, 791
BspCNI CTCAG 1 cut(s) 574
BspEI TCCGGA 1 cut(s) 526
BspFNI CGCG 1 cut(s) 226
BspLI GGNNCC 1 cut(s) 352
BspMI ACCTGC 1 cut(s) 467
BspPI GGATC 2 cut(s) 751, 1007
BspT107I GGYRCC 1 cut(s) 350
BspTNI GGTCTC 2 cut(s) 307, 636
BsrBI CCGCTC 1 cut(s) 323
BsrFI RCCGGY 2 cut(s) 344, 627
BsrGI TGTACA 1 cut(s) 1006
BsrI ACTGG 1 cut(s) 414
BssAI RCCGGY 2 cut(s) 344, 627
BssECI CCNNGG 2 cut(s) 176, 809
BssMI GATC 4 cut(s) 495, 743, 928, 1012
BssNI GRCGYC 5 cut(s) 202, 239, 318, 351, 411
BssT1I CCWWGG 1 cut(s) 176
Bst2UI CCWGG 1 cut(s) 810
Bst4CI ACNGT 3 cut(s) 385, 426, 907
Bst6I CTCTTC 1 cut(s) 377
BstACI GRCGYC 5 cut(s) 202, 239, 318, 351, 411
BstAUI TGTACA 1 cut(s) 1006
BstBAI YACGTR 1 cut(s) 985
BstC8I GCNNGC 4 cut(s) 332, 346, 545, 849
BstDEI CTNAG 2 cut(s) 516, 582
BstDSI CCRYGG 1 cut(s) 176
BstF5I GGATG 3 cut(s) 553, 721, 727
BstFNI CGCG 1 cut(s) 226
BstH2I RGCGCY 2 cut(s) 117, 354
BstHHI GCGC 3 cut(s) 116, 228, 353
BstKTI GATC 4 cut(s) 498, 746, 931, 1015
BstMAI GTCTC 4 cut(s) 212, 307, 636, 867
BstMBI GATC 4 cut(s) 495, 743, 928, 1012
BstMWI GCNNNNNNNGC 4 cut(s) 162, 350, 404, 621
BstNI CCWGG 1 cut(s) 810
BstNSI RCATGY 1 cut(s) 624
BstSCI CCNGG 1 cut(s) 808
BstUI CGCG 1 cut(s) 226
BstV1I GCAGC 4 cut(s) 240, 611, 692, 874
BstX2I RGATCY 1 cut(s) 1012
BstXI CCANNNNNNTGG 1 cut(s) 177
BstYI RGATCY 1 cut(s) 1012
BsuI GTATCC 1 cut(s) 671
BsuRI GGCC 6 cut(s) 79, 267, 365, 458, 502, 791
BtgI CCRYGG 1 cut(s) 176
BtgZI GCGATG 1 cut(s) 379
BtrI CACGTC 1 cut(s) 120
BtsCI GGATG 3 cut(s) 553, 721, 727
BveI ACCTGC 1 cut(s) 467
Cac8I GCNNGC 4 cut(s) 332, 346, 545, 849
CfoI GCGC 3 cut(s) 116, 228, 353
Cfr10I RCCGGY 2 cut(s) 344, 627
Cfr13I GGNCC 2 cut(s) 4, 266
CseI GACGC 3 cut(s) 228, 326, 419
Csp6I GTAC 1 cut(s) 1007
CspCI CAANNNNNGTGG 2 cut(s) 966, 1001
CviAII CATG 6 cut(s) 50, 177, 361, 597, 621, 936
CviQI GTAC 1 cut(s) 1007
DdeI CTNAG 2 cut(s) 516, 582
DinI GGCGCC 1 cut(s) 352
DpnI GATC 4 cut(s) 497, 745, 930, 1014
DpnII GATC 4 cut(s) 495, 743, 928, 1012
EaeI YGGCCR 3 cut(s) 363, 456, 789
Eam1104I CTCTTC 1 cut(s) 377
EarI CTCTTC 1 cut(s) 377
Eco130I CCWWGG 1 cut(s) 176
Eco31I GGTCTC 2 cut(s) 307, 636
Eco47I GGWCC 1 cut(s) 4
Eco57I CTGAAG 1 cut(s) 650
EcoRI GAATTC 1 cut(s) 244
EcoRII CCWGG 1 cut(s) 808
EcoT14I CCWWGG 1 cut(s) 176
EcoT22I ATGCAT 1 cut(s) 555
EgeI GGCGCC 1 cut(s) 352
EheI GGCGCC 1 cut(s) 352
ErhI CCWWGG 1 cut(s) 176
Esp3I CGTCTC 1 cut(s) 212
FaeI CATG 6 cut(s) 53, 180, 364, 600, 624, 939
FaqI GGGAC 1 cut(s) 197
FatI CATG 6 cut(s) 49, 176, 360, 596, 620, 935
FauI CCCGC 3 cut(s) 142, 223, 231
FbaI TGATCA 1 cut(s) 928
Fnu4HI GCNGC 6 cut(s) 229, 321, 349, 625, 681, 863
FokI GGATG 3 cut(s) 540, 728, 734
Fsp4HI GCNGC 6 cut(s) 229, 321, 349, 625, 681, 863
FspBI CTAG 2 cut(s) 473, 635
GlaI GCGC 3 cut(s) 115, 227, 352
GluI GCNGC 6 cut(s) 229, 321, 349, 625, 681, 863
GsuI CTGGAG 1 cut(s) 595
HaeII RGCGCY 2 cut(s) 117, 354
HaeIII GGCC 6 cut(s) 79, 267, 365, 458, 502, 791
HapII CCGG 4 cut(s) 345, 527, 628, 1016
HgaI GACGC 3 cut(s) 228, 326, 419
HhaI GCGC 3 cut(s) 116, 228, 353
Hin1I GRCGYC 5 cut(s) 202, 239, 318, 351, 411
Hin1II CATG 6 cut(s) 53, 180, 364, 600, 624, 939
Hin6I GCGC 3 cut(s) 114, 226, 351
HinP1I GCGC 3 cut(s) 114, 226, 351
HincII GTYRAC 1 cut(s) 21
HindII GTYRAC 1 cut(s) 21
HinfI GANTC 7 cut(s) 181, 523, 559, 571, 602, 915, 965
HpaII CCGG 4 cut(s) 345, 527, 628, 1016
HphI GGTGA 4 cut(s) 114, 532, 710, 911
Hpy166II GTNNAC 2 cut(s) 21, 422
Hpy188III TCNNGA 3 cut(s) 429, 527, 771
Hpy8I GTNNAC 2 cut(s) 21, 422
Hpy99I CGWCG 6 cut(s) 204, 207, 244, 317, 320, 407
HpyAV CCTTC 4 cut(s) 56, 524, 674, 846
HpyCH4III ACNGT 3 cut(s) 385, 426, 907
HpyCH4IV ACGT 4 cut(s) 119, 202, 446, 984
HpyCH4V TGCA 4 cut(s) 553, 680, 739, 862
HpyF10VI GCNNNNNNNGC 4 cut(s) 162, 350, 404, 621
HpyF3I CTNAG 2 cut(s) 516, 582
HpySE526I ACGT 4 cut(s) 119, 202, 446, 984
Hsp92I GRCGYC 5 cut(s) 202, 239, 318, 351, 411
Hsp92II CATG 6 cut(s) 53, 180, 364, 600, 624, 939
HspAI GCGC 3 cut(s) 114, 226, 351
KasI GGCGCC 1 cut(s) 350
Kpn2I TCCGGA 1 cut(s) 526
KroI GCCGGC 1 cut(s) 344
KroNI GCCGGC 1 cut(s) 346
Ksp22I TGATCA 1 cut(s) 928
Kzo9I GATC 4 cut(s) 495, 743, 928, 1012
Lsp1109I GCAGC 4 cut(s) 240, 611, 692, 874
LweI GCATC 5 cut(s) 240, 540, 562, 712, 726
MaeI CTAG 2 cut(s) 473, 635
MaeII ACGT 4 cut(s) 119, 202, 446, 984
MaeIII GTNAC 6 cut(s) 28, 120, 257, 460, 616, 899
MalI GATC 4 cut(s) 497, 745, 930, 1014
MbiI CCGCTC 1 cut(s) 323
MboI GATC 4 cut(s) 495, 743, 928, 1012
MboII GAAGA 2 cut(s) 364, 554
MflI RGATCY 1 cut(s) 1012
MlsI TGGCCA 1 cut(s) 791
MluCI AATT 6 cut(s) 60, 171, 244, 283, 686, 891
MluNI TGGCCA 1 cut(s) 791
Mly113I GGCGCC 1 cut(s) 351
MlyI GAGTC 3 cut(s) 175, 532, 565
MmeI TCCRAC 2 cut(s) 223, 330
MnlI CCTC 6 cut(s) 257, 364, 380, 749, 905, 1004
Mox20I TGGCCA 1 cut(s) 791
Mph1103I ATGCAT 1 cut(s) 555
MreI CGCCGGCG 1 cut(s) 344
MroI TCCGGA 1 cut(s) 526
MroNI GCCGGC 1 cut(s) 344
MscI TGGCCA 1 cut(s) 791
MseI TTAA 1 cut(s) 947
Msp20I TGGCCA 1 cut(s) 791
MspI CCGG 4 cut(s) 345, 527, 628, 1016
MspR9I CCNGG 1 cut(s) 810
Mva1269I GAATGC 1 cut(s) 545
MvaI CCWGG 1 cut(s) 810
MvnI CGCG 1 cut(s) 226
MwoI GCNNNNNNNGC 4 cut(s) 162, 350, 404, 621
NaeI GCCGGC 1 cut(s) 346
NarI GGCGCC 1 cut(s) 351
NcoI CCATGG 1 cut(s) 176
NdeII GATC 4 cut(s) 495, 743, 928, 1012
NgoMIV GCCGGC 1 cut(s) 344
NlaIII CATG 6 cut(s) 53, 180, 364, 600, 624, 939
NlaIV GGNNCC 1 cut(s) 352
NmuCI GTSAC 5 cut(s) 28, 120, 460, 616, 899
NsiI ATGCAT 1 cut(s) 555
NspI RCATGY 1 cut(s) 624
PcsI WCGNNNNNNNCGW 1 cut(s) 196
PctI GAATGC 1 cut(s) 545
PdiI GCCGGC 1 cut(s) 346
PfeI GAWTC 4 cut(s) 559, 602, 915, 965
PflFI GACNNNGTC 1 cut(s) 204
PkrI GCNGC 6 cut(s) 230, 322, 350, 626, 682, 864
PleI GAGTC 3 cut(s) 175, 531, 565
PluTI GGCGCC 1 cut(s) 354
PpsI GAGTC 3 cut(s) 175, 531, 565
Ppu21I YACGTR 1 cut(s) 985
PshBI ATTAAT 1 cut(s) 947
Psp6I CCWGG 1 cut(s) 808
PspGI CCWGG 1 cut(s) 808
PspN4I GGNNCC 1 cut(s) 352
PspPI GGNCC 2 cut(s) 4, 266
PsuI RGATCY 1 cut(s) 1012
PsyI GACNNNGTC 1 cut(s) 204
RsaI GTAC 1 cut(s) 1008
RsaNI GTAC 1 cut(s) 1007
SaqAI TTAA 1 cut(s) 947
SatI GCNGC 6 cut(s) 229, 321, 349, 625, 681, 863
Sau3AI GATC 4 cut(s) 495, 743, 928, 1012
Sau96I GGNCC 2 cut(s) 4, 266
SchI GAGTC 3 cut(s) 175, 532, 565
ScrFI CCNGG 1 cut(s) 810
SfaNI GCATC 5 cut(s) 240, 540, 562, 712, 726
SfoI GGCGCC 1 cut(s) 352
SgrAI CRCCGGYG 1 cut(s) 344
SinI GGWCC 1 cut(s) 4
Sse9I AATT 6 cut(s) 60, 171, 244, 283, 686, 891
SsiI CCGC 7 cut(s) 149, 163, 216, 224, 321, 348, 379
SspDI GGCGCC 1 cut(s) 350
SspI AATATT 1 cut(s) 858
SspMI CTAG 2 cut(s) 473, 635
StyD4I CCNGG 1 cut(s) 808
StyI CCWWGG 1 cut(s) 176
TaaI ACNGT 3 cut(s) 385, 426, 907
TaiI ACGT 4 cut(s) 122, 205, 449, 987
TaqI TCGA 6 cut(s) 140, 242, 494, 537, 754, 963
TasI AATT 6 cut(s) 60, 171, 244, 283, 686, 891
TatI WGTACW 1 cut(s) 1006
TauI GCSGC 2 cut(s) 323, 351
TfiI GAWTC 4 cut(s) 559, 602, 915, 965
Tru1I TTAA 1 cut(s) 947
Tru9I TTAA 1 cut(s) 947
TseFI GTSAC 5 cut(s) 28, 120, 460, 616, 899
TseI GCWGC 4 cut(s) 228, 624, 680, 862
Tsp45I GTSAC 5 cut(s) 28, 120, 460, 616, 899
TspDTI ATGAA 3 cut(s) 38, 779, 933
Tth111I GACNNNGTC 1 cut(s) 204
VpaK11BI GGWCC 1 cut(s) 4
VspI ATTAAT 1 cut(s) 947
XapI RAATTY 3 cut(s) 60, 244, 686
XceI RCATGY 1 cut(s) 624
XspI CTAG 2 cut(s) 473, 635
ZraI GACGTC 1 cut(s) 203
Zsp2I ATGCAT 1 cut(s) 555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.