pycom13g09320

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0003663
Physical Location & Seq
Reverse (-)
6241842 .. 6250420
8579 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 5814 bp
ATGCGTGCCATTCTGATGTCTGAGAAACCTGGTTTGAAGCAGGTTCTGGAGGACTCGGCAGCTGAGGCGGAGGGAGATCGACCGATTCGAGGCAGTCCAATTGCGAATGGGTTGGCCAAGGATTCGAATGCAATTCGAGACGACGTCGCCGAGCCGGTGAATCAGGAATTAGGGTCAGCGTGGCCGGTGGCGGAATCGGTGGCAGACGGTGATAACCAGGTGGCGGAAGATAAAGGCAAAGTGACGGAGGATTCGGGGAAAGAGGAGTTTGTGGATTGCTCGGAGGATTACGCGATGGATGAGTTGGATCGCCTGCGGCTTTTGCTGGATACTACTGTTGGCGAGAAGGAAAGTTTGGCGCGCCAATTCGATGAAGAAAGAGAGGCTTTTGCTAGAGAAATTGCTAGTCTTCGGTTTCAGCTGAATGCTCTTACTGATCAACAGCCATTGATTGACGAAAGCGGTAATTTTGTTGATGACAAAGAAAGTAGGGAAGATGATAAGGGTACGGATACCCGGTGGAGTGAGTTGATAACTGAATGCTCTGGACTTGTTAAAACGGCTTTGGAGAAACGGTTGCAGACTGAGGCCGCGGTGAGAGAACTTGATGGAGTTGTGTTTAAGAAGGATCAATCGATTGAGGAACTCAATGCCAAGATCAATGAGTTTTCGGTTTTGAATGACGTGGTTGCAATTTTTTTGAATTCTGCTCAGAGGTCTGTGGAAGTGTCATCTGAGGTTCAGATTGAGAAGGATACGCACATTGAAGTTGTGACAAACAGGATGCTGGCTTCCCTTGGGGGAGTCGTTGATCAACAAGAAATGTTGGATGGTTCTATCGGTGGAAAATTAGTACATGTTGAGCAAGGCACTTCTATGCTAATTGAGAAGTTCACCAGGATGCTTTCGGAAATTGAACAACTTAGACAGTCTTTGCCGGAAGCCAGGTCAGATCATAAATCGCAGGAATTAGGAGGGGTTTTTGCTGCTGCCTGTTATGAGTTACTTGAGCTCAAAAGAAAGGAAGCAGAGTTTGTCGAAAGATTGGGCCATCTAGAAGACGAAAATAGGAAATTGATAGAAGAACTTGAAAATCAAAAAGGGATAGTTGAGGCGGTGAATGCGGATCTAGGACAAACAAAAATGGAACTGGAGCAGGAGAAGCATAGGTGCTCTAATACCAGAGAGAAGCTTACTATGGCCATTACAAAAGGGAAGGCATTGGTGCAGCAACGGGACTCATTAAAGCAGTCCATTGCTGAAAAAACGAGTGAGCTTGAGAAGTGTTTGATTGAATTGCAAGAGAAGTCAAGTGCACTTGAAGCTGCTGAGTTAACCAAGGAGGAATTGATTAGAAGCGAAAATTCAATTGCATCCTTGCAAGAAATAGTATACCAGAAGAATGTAATCATTGAAAGTTTGGAGGACATTATGTCTCAGACCGGTGTACCTGAAGAACTTCAATCTATGGATATTCTAGAGAAACTTAGATGGCTTTCGGATGAAAATGATAAACTCAAGGGAATTTCCCTTGAATTCAAAAACTTGAGAGATGCTATGCATGCAATTGATCTACCAGAAGTTATTTCATCTTCTGACTTAGAGTCTCAAGTAAATTGGCTTAGGGAATCGGTTTCTCAGGCCAAAGAGGAAGTGCTTATGTTGTGCGATGAAATTACTGCAACTAAGGAAGTGGCACATAAAAATATCGACCACTTAACTGATTCACTTTCAGCTGAGTTGCAGGCAAAGGAATATCTCCAAGCAGAGCTGGATAATCGGACATCCGAATACCAAGAGATTGTCAAGAAAGAGCAACAGGTGTCTTTAGAGAAGGCTGATATGGCGAAAGAGGAAGTACTTATGTTGCGTGATAAAATTACTGCAACTAAGGAAGTGGCGCGTAAAAATATTGAAGACTTAACTGCTTCACTTTCAGATGAATTGCAGGCAAAGGAATATCTCCAAGCAGAGCTGGATAATCTGACATCTGAATACCAAGAGATTGTCAAGAAAGAGCAACAGGTGTCTTCAGAGAAGGCTGATATGGTCAGAATGTTACTCGATGTCTCAGGAGTTCTAGTGGACAATGAAGAGGTCTATCAACCTTCGTTGGACCCTGCCTTGCTTGTTGACAGATGCATTGGGAAGATAAAAGAAGAGAGTAATGCCTCACTTGATTCTCCAAAGGTTGATGCTGAGTTGTTTGAAACAATTCAAAGCCACCTGTATGTAAGGGATCAGAAATTGATGCTGTGTGAGACTTTACTAGAAGAGGAGACACTGGTGAGGTCAGAGGTGAATAACCTGTCAAATGAGTTGCGGGCTGTATCTCAAAAACTTGCAGCATTGGAAGAGGAAAAAGGGAACCTGCAGAGAGATTTTGAACGATCAGAGGAGAAAAATACTGTGCTAAGGGAGAAGTTATCCATGGCAGTCAAGAAAGGTAAGGGACTGGTTCAAGACCGGGAAAACTTGAAACATCTTTTAGATGACAAGAAATCAGAAATTGAGAAGTTACAGCTTGAGTTACAGCAGGAACAGTTGGCACTTGCTGAATGCAGGGACAAGATAAGTAGTTTGTCTGCTTATGTACACCGCATCCCAAAGTTAGATGCTGATCTAGTTGCTATGAAGGAACAACGGGATCAACTTGAACAGTTCTTAGTGGAGAGTAATAACATGTTACAGAGAGTAATTGAATGTATTGATGGCATTGCTCTTCCTGTCGATTCAATTTTTGAAGAGCCTGTGGGGAAGGTGAATTTCATTGCAGGATACATAAGTGAATGTCAGGATGCCAAGGAAAATTCAGAGCATGAGTTGAGTAAAGTAAAAGAGGAAGTCAATAATCTGGCTGGTAAGTTAGCAGAAACTCACTCAACGTTAAAGTCTCTGGAAAATGAATTGTCGGTTGCAGAGAGTGATATTTCTCTGCTTGCTGAACAAAAGAGGGAAATTGAATTGGGTAAGACCAATGTTGAAAAAGACTTGGAGAAAGCAATTGAGGAAGCTATGTCTCAAGCTAGCAAGTACAATGAAGTTTGTGCATCTAAAAAGTCACTGGAAGAGGCGTTATCAATTGCAGAAAATAGTATATCTGTGCTTGTTAGTGAAAAAGAAGGAGCTTTAGTTGGTAGAGCTTCTGCAGAGAAAGAGCTAGAGAAAGTGAAAGAGGAAGTTGATACTCAGACTAGCAAACTAACTGAGGCCAATAAGGCCATAAAGTTACTCGAAGATTCCCTATCACTGGTACAGACCAATGTTTCTTTGCTTACCGAACAAAACAATGAAGTTCACATTGGTAGGACCAATTTAGAGGTTGAACTAAAGAAACTGCAAGAGGAAGCTAGGATCCAAGATAACAAGCTAGCAGATGCCCAGGCAACCATAAAATCACTGGAAGATGCATTGCTGAAGGCTGAGAATGATATTTCTGTCCTTCAAGGTGAAAAGAAAAATGCTGAAGACGAAATATTGACACTTAATTCCAAGTTAAATACACAAGACGAAGAGTTGTCTGGAACTAATTGCAGCACAGAAAGCAGGTCCATAGAGCTAAGTGGCCACCTTCATAATCTTCACCTGCTAATGAAAGATGATACTCTGTTGGCCACTATGAAAAGATGTTTCGAGAAGAAATTTGAGAGCCTGAAAGATGTGGACCTCATTCTTAGAAATATAAAGGACAATTGTGTTTCCATGAATTCAGAAGAGCTGCAAAGACATCAAGTGTTGGAGGAAGATTCATATGTGACAAAGTCTTTCTCAGATGGCATTGACACTATATCCAGTGTGGAAAAGGTTACTGGTGAGGCCAGTGTTGCAGATGCTGATATGTCTTCATGTTTGAAGCAGACTGTGGAAAGGTTCCAGATGCGAGAAGATGTTCTTGCCGAAATTTTTGAATGTTTATCTTCTACTGTAGATGGGTTTATTGCAAATCTGTTGAGAAATCTACAGGCAGTAAGGGATGAAGTAATAGCTAGGTTCGAGAACATGGAGTCTGTCAAACAACAAGCAACTAATCTGGAAATATGTAAACAGGAACAGGAGAATACAATAGCCATATTAGAAAATGATCTTAAGTCTCTACTTTCTGCTTGTACCACTGCTATCGGAGAACTTCAATTTGGAGTCGAGAACAATTTACTTGAACTGAGCTCTGTTCCTGAGCTTGAAAAATTGAAACATAATTTGCCCCAGGAAACAGTAATCAGTGGAGAGACCACAGAAACAGATGAGCAAGGTCTACAGCACAGCAAATATGGAAAAACTGCAGAAATGTTGTATGTTTCTATTAGAAAAGTTAAAGCTTTGATTAAACAGTTTGAGAGAACAAGTAAAGTGGCAGCCTCTACAATTGAAGATCTGCAGTGTAAACTGACAGAAGCTAGGACGATTTCTAAGAAAGCCATTGAAGAAAGGGATCTAGGACAAAATAGGATTTCCAAGTTGGACGCTGATGTAGAAGCATTACAGAATTCGTGCAGTAAGCTGACACTTAGACTAGAGGATTATCAATCCCAAGAGGATAAGTTTAATGAAAAAGAGGTGGAAGTTTTATCACTGCGCAATGCTTTGTCGATGAAAGAACAAGAGGCTGAAGACTCACTCCTGTCAGCATCTGAAATTAAAACCCTTTTTGACAAGATCGGCAGGATTGAAATCCCAGAGTCACATGGAGGAGACTTGGAGCCCCACAATTCAGCTCATGTGAAGAAGCTCTCTTACGTTATTGATAATATTACTGACCATCAGTATCGGATAAACTTATTGTCTTATGAAAATGAAGAACTGCAGTCAACTCTTGGGACTAGGAATCTCGAAATTGAACAATTGAAGGAGGAGGCTGAAAGTTACGACAGAGATAGACAAGATAGAGAGAAGATGAAAAATGAATTGTCTGTGCTCATATATAGTTTGGAAAAAATTATAGATATGTCAGGAGGTAGTGATTTAGTTGTAGACCAGAAATCTTCTGGTGTGACTGGACTTCTATCAGTATTGGAGAAGCAGGTTAGGGCTTTACAATCGGAGTCTGAAAGTTCAAAATCAAAAGCCCAGGAACTTGGTACTAAGTTGGACGAGAGCCAAAAGGTCGTGGAGGAATTATCGACCAAGGTTAATTCACTTCAAGGGCGAGCTGCTCAGCCAGAGATTGTCCAGGAAAGGAGTATCTTTGAAGCACCTACTACTGGTTCAGAGATATCTGAAATTGAAGATGGGGGATCGCATGGAAAGAACGCAATATCTCCTGTCCAATCAGCAGCTCATGTGCGAACTATGCGGAAGGGTTCAACTGAACATCTCGCAATTGAAATTGGTTCTGAATCTACTCGTTTAATCAACAATGAAGAAACCGATGAGGACAAAGGTCATGTATTCAAATCTCTCAATGCATCTGGTCTCGTACCAAGACAAGGAAAGTTGATTGCAGATCGAATCGATGGAATTTGGGTATCCGGCGGTCGGGTTTTGATGAGTCGCCCGAGGGCAAGGCTCGGACTGATCGCCTACTGGCTCTTCTTGCATCTGTGGCTGCATCTCATGTTGCATCAGTTGAAGGATTGCCGGTCTTTGACTGCTCATTGGAAGTATCATGTCCTTACATCACTTCAACCTTTGGATTACATAATCGTTGCATTTTTGCCCGGGGTCAGCGAGGAACTTCTTTTTCGGGGTGCCTTGCTACCACTTTTTGGATCCAATTGGAGGAGCGCCTTGATAGTCGCCATCATTTTTGGTGTTCTGCACCTGGGCAGTGGCCGAAAGTATTCCTTTGCTGTCTGGTACTTATCCTCTTATCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1938

Amino Acids

217.47

Weight (kDa)

4.76

Isoelectric Point (pI)

49.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rce1-like PF02517 1875 - 1925 2.5e-08 Type II CAAX prenyl endopeptidase Rce1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3594
AatII GACGTC 1 cut(s) 147
Acc16I TGCGCA 1 cut(s) 4574
Acc36I ACCTGC 5 cut(s) 31, 2379, 3537, 3594, 5008
AccB1I GGYRCC 1 cut(s) 5684
AccB7I CCANNNNNTGG 1 cut(s) 5702
AccI GTMKAC 3 cut(s) 1392, 4252, 4968
AccII CGCG 4 cut(s) 293, 361, 593, 1903
AclI AACGTT 1 cut(s) 2885
AcoI YGGCCR 6 cut(s) 114, 182, 1200, 3565, 3612, 5767
AcuI CTGAAG 5 cut(s) 1473, 2016, 3437, 3486, 4626
AcyI GRCGYC 1 cut(s) 144
AfiI CCNNNNNNNGG 8 cut(s) 89, 223, 3250, 5198, 5423, 5472, 5702, 5715
AflII CTTAAG 1 cut(s) 4085
AflIII ACRYGT 2 cut(s) 856, 2682
AgeI ACCGGT 1 cut(s) 1442
AhdI GACNNNNNGTC 2 cut(s) 1432, 1603
AjiI CACGTC 1 cut(s) 685
AjnI CCWGG 9 cut(s) 28, 216, 896, 944, 3381, 4203, 5064, 5166, 5757
AjuI GAANNNNNNNTTGG 6 cut(s) 338, 370, 3285, 3317, 4116, 4148
Alw21I GWGCWC 5 cut(s) 1014, 1175, 1318, 4166, 4914
Alw44I GTGCAC 1 cut(s) 1314
AlwNI CAGNNNCTG 3 cut(s) 46, 3833, 4628
Ama87I CYCGRG 2 cut(s) 5491, 5654
ApaLI GTGCAC 1 cut(s) 1314
ArsI GACNNNNNNTTYG 2 cut(s) 235, 267
AscI GGCGCGCC 1 cut(s) 359
AsiGI ACCGGT 1 cut(s) 1442
Asp700I GAANNNNTTC 4 cut(s) 1458, 2214, 3888, 5776
AspLEI GCGC 5 cut(s) 361, 363, 1903, 4575, 5723
AspS9I GGNCC 5 cut(s) 1048, 2116, 3309, 3549, 3664
AsuC2I CCSGG 4 cut(s) 517, 2468, 5655, 5656
AsuII TTCGAA 1 cut(s) 125
AsuNHI GCTAGC 2 cut(s) 3026, 3370
AvaI CYCGRG 2 cut(s) 5491, 5654
AvaII GGWCC 4 cut(s) 2116, 3309, 3549, 3664
BaeGI GKGCMC 1 cut(s) 1318
BaeI ACNNNNGTAYC 6 cut(s) 321, 354, 1431, 1464, 2770, 2803
BalI TGGCCA 4 cut(s) 116, 1202, 3567, 3614
BamHI GGATCC 2 cut(s) 3354, 5705
BanI GGYRCC 1 cut(s) 5684
BanII GRGCYC 3 cut(s) 1014, 4166, 4701
BarI GAAGNNNNNNTAC 2 cut(s) 4715, 4747
BbsI GAAGAC 7 cut(s) 401, 1065, 1923, 2022, 3474, 3834, 4614
Bbv12I GWGCWC 5 cut(s) 1014, 1175, 1318, 4166, 4914
BbvCI CCTCAGC 1 cut(s) 63
BceAI ACGGC 1 cut(s) 576
BciT130I CCWGG 9 cut(s) 30, 218, 898, 946, 3383, 4205, 5066, 5168, 5759
BciVI GTATCC 5 cut(s) 322, 505, 748, 2771, 5473
BclI TGATCA 2 cut(s) 436, 811
BcnI CCSGG 4 cut(s) 517, 2468, 5655, 5656
BfmI CTRYAG 8 cut(s) 2372, 3147, 3924, 3959, 4253, 4278, 4373, 4799
BfoI RGCGCY 1 cut(s) 5724
BfrI CTTAAG 1 cut(s) 4085
BfuAI ACCTGC 5 cut(s) 31, 2379, 3537, 3594, 5008
BfuI GTATCC 5 cut(s) 322, 505, 748, 2771, 5473
BglI GCCNNNNNGGC 1 cut(s) 3218
BglII AGATCT 1 cut(s) 4369
BlpI GCTNAGC 1 cut(s) 5151
BmcAI AGTACT 1 cut(s) 1860
Bme18I GGWCC 4 cut(s) 2116, 3309, 3549, 3664
BmeRI GACNNNNNGTC 2 cut(s) 1432, 1603
BmeT110I CYCGRG 2 cut(s) 5491, 5654
BmgBI CACGTC 1 cut(s) 685
BmgT120I GGNCC 5 cut(s) 1048, 2116, 3309, 3549, 3664
BmiI GGNNCC 7 cut(s) 2118, 2369, 3356, 3872, 4698, 5686, 5707
BmtI GCTAGC 2 cut(s) 3030, 3374
BoxI GACNNNNGTC 1 cut(s) 5376
BpiI GAAGAC 7 cut(s) 401, 1065, 1923, 2022, 3474, 3834, 4614
BpmI CTGGAG 2 cut(s) 68, 1172
Bpu10I CCTNAGC 4 cut(s) 63, 1622, 2414, 4173
Bpu1102I GCTNAGC 1 cut(s) 5151
Bpu14I TTCGAA 1 cut(s) 125
BpuEI CTTGAG 7 cut(s) 1028, 1298, 1502, 1567, 1593, 2546, 3006
BpuMI CCSGG 4 cut(s) 517, 2468, 5655, 5656
Bsa29I ATCGAT 2 cut(s) 635, 5448
BsaBI GATNNNNATC 2 cut(s) 2619, 4667
BsaHI GRCGYC 1 cut(s) 144
BsaI GGTCTC 2 cut(s) 4220, 5414
BsaWI WCCGGW 1 cut(s) 1442
BsaXI ACNNNNNCTCC 6 cut(s) 560, 590, 2067, 2097, 3117, 3147
Bsc4I CCNNNNNNNGG 8 cut(s) 89, 223, 3250, 5198, 5423, 5472, 5702, 5715
Bse118I RCCGGY 4 cut(s) 154, 184, 1442, 5574
Bse3DI GCAATG 5 cut(s) 1254, 2715, 2769, 3410, 4582
Bse8I GATNNNNATC 2 cut(s) 2619, 4667
BseBI CCWGG 9 cut(s) 30, 218, 898, 946, 3383, 4205, 5066, 5168, 5759
BseCI ATCGAT 2 cut(s) 635, 5448
BseJI GATNNNNATC 2 cut(s) 2619, 4667
BseLI CCNNNNNNNGG 8 cut(s) 89, 223, 3250, 5198, 5423, 5472, 5702, 5715
BseMI GCAATG 5 cut(s) 1254, 2715, 2769, 3410, 4582
BsePI GCGCGC 1 cut(s) 359
BseRI GAGGAG 6 cut(s) 278, 2291, 2411, 4701, 4862, 5731
BseSI GKGCMC 1 cut(s) 1318
BsgI GTGCAG 3 cut(s) 1247, 4510, 5738
Bsh1236I CGCG 4 cut(s) 293, 361, 593, 1903
Bsh1285I CGRYCG 2 cut(s) 83, 5473
BshNI GGYRCC 1 cut(s) 5684
BshTI ACCGGT 1 cut(s) 1442
BshVI ATCGAT 2 cut(s) 635, 5448
BsiEI CGRYCG 2 cut(s) 83, 5473
BsiHKAI GWGCWC 5 cut(s) 1014, 1175, 1318, 4166, 4914
BsiHKCI CYCGRG 2 cut(s) 5491, 5654
BsiSI CCGG 9 cut(s) 155, 185, 517, 938, 1443, 2467, 5466, 5575, 5655
BslFI GGGAC 4 cut(s) 1250, 2466, 2579, 4828
BslI CCNNNNNNNGG 8 cut(s) 89, 223, 3250, 5198, 5423, 5472, 5702, 5715
BsmBI CGTCTC 1 cut(s) 132
BsmFI GGGAC 4 cut(s) 1250, 2466, 2579, 4828
BsmI GAATGC 5 cut(s) 133, 430, 545, 1126, 2564
Bso31I GGTCTC 2 cut(s) 4220, 5414
BsoBI CYCGRG 2 cut(s) 5491, 5654
Bsp119I TTCGAA 1 cut(s) 125
Bsp1286I GDGCHC 6 cut(s) 1014, 1175, 1318, 4166, 4701, 4914
Bsp1407I TGTACA 1 cut(s) 2593
Bsp1720I GCTNAGC 1 cut(s) 5151
Bsp19I CCATGG 1 cut(s) 2430
BspDI ATCGAT 2 cut(s) 635, 5448
BspFNI CGCG 4 cut(s) 293, 361, 593, 1903
BspLI GGNNCC 7 cut(s) 2118, 2369, 3356, 3872, 4698, 5686, 5707
BspMAI CTGCAG 5 cut(s) 2376, 3151, 4282, 4377, 4803
BspMI ACCTGC 5 cut(s) 31, 2379, 3537, 3594, 5008
BspOI GCTAGC 2 cut(s) 3030, 3374
BspQI GCTCTTC 4 cut(s) 2727, 2739, 3708, 5531
BspT104I TTCGAA 1 cut(s) 125
BspT107I GGYRCC 1 cut(s) 5684
BspTI CTTAAG 1 cut(s) 4085
BspTNI GGTCTC 2 cut(s) 4220, 5414
BsrDI GCAATG 5 cut(s) 1254, 2715, 2769, 3410, 4582
BsrFI RCCGGY 4 cut(s) 154, 184, 1442, 5574
BsrGI TGTACA 1 cut(s) 2593
BssAI RCCGGY 4 cut(s) 154, 184, 1442, 5574
BssHII GCGCGC 1 cut(s) 359
BssNAI GTATAC 1 cut(s) 1393
BssNI GRCGYC 1 cut(s) 144
BssT1I CCWWGG 6 cut(s) 117, 796, 1338, 2430, 2802, 5121
Bst1107I GTATAC 1 cut(s) 1393
Bst2UI CCWGG 9 cut(s) 30, 218, 898, 946, 3383, 4205, 5066, 5168, 5759
BstACI GRCGYC 1 cut(s) 144
BstAFI CTTAAG 1 cut(s) 4085
BstAUI TGTACA 1 cut(s) 2593
BstBI TTCGAA 1 cut(s) 125
BstDSI CCRYGG 2 cut(s) 591, 2430
BstFNI CGCG 4 cut(s) 293, 361, 593, 1903
BstH2I RGCGCY 1 cut(s) 5724
BstHHI GCGC 5 cut(s) 361, 363, 1903, 4575, 5723
BstMCI CGRYCG 2 cut(s) 83, 5473
BstNI CCWGG 9 cut(s) 30, 218, 898, 946, 3383, 4205, 5066, 5168, 5759
BstNSI RCATGY 3 cut(s) 860, 1565, 2686
BstPAI GACNNNNGTC 1 cut(s) 5376
BstSFI CTRYAG 8 cut(s) 2372, 3147, 3924, 3959, 4253, 4278, 4373, 4799
BstSLI GKGCMC 1 cut(s) 1318
BstUI CGCG 4 cut(s) 293, 361, 593, 1903
BstV2I GAAGAC 7 cut(s) 401, 1065, 1923, 2022, 3474, 3834, 4614
BstX2I RGATCY 5 cut(s) 1126, 3354, 4369, 4429, 5705
BstXI CCANNNNNNTGG 1 cut(s) 5072
BstYI RGATCY 5 cut(s) 1126, 3354, 4369, 4429, 5705
BstZ17I GTATAC 1 cut(s) 1393
Bsu15I ATCGAT 2 cut(s) 635, 5448
BsuI GTATCC 5 cut(s) 322, 505, 748, 2771, 5473
BsuTUI ATCGAT 2 cut(s) 635, 5448
BtgI CCRYGG 2 cut(s) 591, 2430
BtgZI GCGATG 2 cut(s) 308, 1683
BtrI CACGTC 1 cut(s) 685
BtsI GCAGTG 4 cut(s) 4110, 4382, 4568, 5770
BveI ACCTGC 5 cut(s) 31, 2379, 3537, 3594, 5008
CaiI CAGNNNCTG 3 cut(s) 46, 3833, 4628
CfoI GCGC 5 cut(s) 361, 363, 1903, 4575, 5723
Cfr10I RCCGGY 4 cut(s) 154, 184, 1442, 5574
Cfr13I GGNCC 5 cut(s) 1048, 2116, 3309, 3549, 3664
Cfr42I CCGCGG 1 cut(s) 594
Cfr9I CCCGGG 1 cut(s) 5654
ClaI ATCGAT 2 cut(s) 635, 5448
CseI GACGC 1 cut(s) 4469
CsiI ACCWGGT 2 cut(s) 28, 216
CspAI ACCGGT 1 cut(s) 1442
CspCI CAANNNNNGTGG 2 cut(s) 4329, 4364
DriI GACNNNNNGTC 2 cut(s) 1432, 1603
EaeI YGGCCR 6 cut(s) 114, 182, 1200, 3565, 3612, 5767
Eam1105I GACNNNNNGTC 2 cut(s) 1432, 1603
EciI GGCGGA 3 cut(s) 83, 206, 239
Ecl136II GAGCTC 2 cut(s) 1012, 4164
Eco130I CCWWGG 6 cut(s) 117, 796, 1338, 2430, 2802, 5121
Eco24I GRGCYC 3 cut(s) 1014, 4166, 4701
Eco31I GGTCTC 2 cut(s) 4220, 5414
Eco32I GATATC 1 cut(s) 5211
Eco47I GGWCC 4 cut(s) 2116, 3309, 3549, 3664
Eco53kI GAGCTC 2 cut(s) 1012, 4164
Eco57I CTGAAG 5 cut(s) 1473, 2016, 3437, 3486, 4626
Eco88I CYCGRG 2 cut(s) 5491, 5654
EcoICRI GAGCTC 2 cut(s) 1012, 4164
EcoRI GAATTC 4 cut(s) 703, 1535, 3706, 4483
EcoRII CCWGG 9 cut(s) 28, 216, 896, 944, 3381, 4203, 5064, 5166, 5757
EcoRV GATATC 1 cut(s) 5211
EcoT14I CCWWGG 6 cut(s) 117, 796, 1338, 2430, 2802, 5121
EcoT22I ATGCAT 4 cut(s) 1563, 2144, 3412, 5404
EcoT38I GRGCYC 3 cut(s) 1014, 4166, 4701
ErhI CCWWGG 6 cut(s) 117, 796, 1338, 2430, 2802, 5121
Esp3I CGTCTC 1 cut(s) 132
FalI AAGNNNNNCTT 6 cut(s) 370, 402, 3876, 3908, 5765, 5797
FaqI GGGAC 4 cut(s) 1250, 2466, 2579, 4828
FauI CCCGC 1 cut(s) 2316
FauNDI CATATG 1 cut(s) 3751
FbaI TGATCA 2 cut(s) 436, 811
FblI GTMKAC 3 cut(s) 1392, 4252, 4968
FriOI GRGCYC 3 cut(s) 1014, 4166, 4701
FspI TGCGCA 1 cut(s) 4574
GlaI GCGC 5 cut(s) 360, 362, 1902, 4574, 5722
GsuI CTGGAG 2 cut(s) 68, 1172
HaeII RGCGCY 1 cut(s) 5724
HapII CCGG 9 cut(s) 155, 185, 517, 938, 1443, 2467, 5466, 5575, 5655
HgaI GACGC 1 cut(s) 4469
HhaI GCGC 5 cut(s) 361, 363, 1903, 4575, 5723
Hin1I GRCGYC 1 cut(s) 144
Hin6I GCGC 5 cut(s) 359, 361, 1901, 4573, 5721
HinP1I GCGC 5 cut(s) 359, 361, 1901, 4573, 5721
HincII GTYRAC 3 cut(s) 1335, 2134, 4806
HindII GTYRAC 3 cut(s) 1335, 2134, 4806
HindIII AAGCTT 2 cut(s) 1190, 4314
HpaI GTTAAC 1 cut(s) 1335
HpaII CCGG 9 cut(s) 155, 185, 517, 938, 1443, 2467, 5466, 5575, 5655
Hpy99I CGWCG 2 cut(s) 146, 149
HpyCH4IV ACGT 4 cut(s) 144, 684, 2885, 4734
HpySE526I ACGT 4 cut(s) 144, 684, 2885, 4734
Hsp92I GRCGYC 1 cut(s) 144
HspAI GCGC 5 cut(s) 359, 361, 1901, 4573, 5721
Ksp22I TGATCA 2 cut(s) 436, 811
KspAI GTTAAC 1 cut(s) 1335
KspI CCGCGG 1 cut(s) 594
LguI GCTCTTC 4 cut(s) 2727, 2739, 3708, 5531
LmnI GCTCC 4 cut(s) 1153, 3125, 4696, 5718
MabI ACCWGGT 2 cut(s) 28, 216
MaeII ACGT 4 cut(s) 144, 684, 2885, 4734
MflI RGATCY 5 cut(s) 1126, 3354, 4369, 4429, 5705
MhlI GDGCHC 6 cut(s) 1014, 1175, 1318, 4166, 4701, 4914
MlsI TGGCCA 4 cut(s) 116, 1202, 3567, 3614
MluNI TGGCCA 4 cut(s) 116, 1202, 3567, 3614
MmeI TCCRAC 6 cut(s) 285, 807, 2094, 3717, 4437, 5064
Mox20I TGGCCA 4 cut(s) 116, 1202, 3567, 3614
Mph1103I ATGCAT 4 cut(s) 1563, 2144, 3412, 5404
MroXI GAANNNNTTC 4 cut(s) 1458, 2214, 3888, 5776
MscI TGGCCA 4 cut(s) 116, 1202, 3567, 3614
MslI CAYNNNNRTG 4 cut(s) 14, 875, 899, 2229
Msp20I TGGCCA 4 cut(s) 116, 1202, 3567, 3614
MspA1I CMGCKG 4 cut(s) 62, 421, 593, 1736
MspCI CTTAAG 1 cut(s) 4085
MspI CCGG 9 cut(s) 155, 185, 517, 938, 1443, 2467, 5466, 5575, 5655
Mva1269I GAATGC 5 cut(s) 133, 430, 545, 1126, 2564
MvaI CCWGG 9 cut(s) 30, 218, 898, 946, 3383, 4205, 5066, 5168, 5759
MvnI CGCG 4 cut(s) 293, 361, 593, 1903
NciI CCSGG 4 cut(s) 517, 2468, 5655, 5656
NcoI CCATGG 1 cut(s) 2430
NdeI CATATG 1 cut(s) 3751
NheI GCTAGC 2 cut(s) 3026, 3370
NlaIV GGNNCC 7 cut(s) 2118, 2369, 3356, 3872, 4698, 5686, 5707
NmeAIII GCCGAG 2 cut(s) 35, 175
NmuCI GTSAC 6 cut(s) 241, 772, 3060, 3754, 4677, 4987
NsbI TGCGCA 1 cut(s) 4574
NsiI ATGCAT 4 cut(s) 1563, 2144, 3412, 5404
NspI RCATGY 3 cut(s) 860, 1565, 2686
NspV TTCGAA 1 cut(s) 125
PaeI GCATGC 1 cut(s) 1565
PalAI GGCGCGCC 1 cut(s) 359
PaqCI CACCTGC 1 cut(s) 3594
PauI GCGCGC 1 cut(s) 359
PciI ACATGT 2 cut(s) 856, 2682
PciSI GCTCTTC 4 cut(s) 2727, 2739, 3708, 5531
PcsI WCGNNNNNNNCGW 2 cut(s) 85, 147
PctI GAATGC 5 cut(s) 133, 430, 545, 1126, 2564
PdmI GAANNNNTTC 4 cut(s) 1458, 2214, 3888, 5776
PflFI GACNNNGTC 2 cut(s) 143, 3760
PflMI CCANNNNNTGG 1 cut(s) 5702
PfoI TCCNGGA 1 cut(s) 5166
PinAI ACCGGT 1 cut(s) 1442
PscI ACATGT 2 cut(s) 856, 2682
PshAI GACNNNNGTC 1 cut(s) 5376
Psp124BI GAGCTC 2 cut(s) 1014, 4166
Psp1406I AACGTT 1 cut(s) 2885
Psp6I CCWGG 9 cut(s) 28, 216, 896, 944, 3381, 4203, 5064, 5166, 5757
PspGI CCWGG 9 cut(s) 28, 216, 896, 944, 3381, 4203, 5064, 5166, 5757
PspN4I GGNNCC 7 cut(s) 2118, 2369, 3356, 3872, 4698, 5686, 5707
PspPI GGNCC 5 cut(s) 1048, 2116, 3309, 3549, 3664
PstI CTGCAG 5 cut(s) 2376, 3151, 4282, 4377, 4803
PstNI CAGNNNCTG 3 cut(s) 46, 3833, 4628
PsuI RGATCY 5 cut(s) 1126, 3354, 4369, 4429, 5705
PsyI GACNNNGTC 2 cut(s) 143, 3760
PteI GCGCGC 1 cut(s) 359
PvuII CAGCTG 3 cut(s) 62, 421, 1736
RseI CAYNNNNRTG 4 cut(s) 14, 875, 899, 2229
SacI GAGCTC 2 cut(s) 1014, 4166
SacII CCGCGG 1 cut(s) 594
SapI GCTCTTC 4 cut(s) 2727, 2739, 3708, 5531
Sau96I GGNCC 5 cut(s) 1048, 2116, 3309, 3549, 3664
ScaI AGTACT 1 cut(s) 1860
SduI GDGCHC 6 cut(s) 1014, 1175, 1318, 4166, 4701, 4914
SexAI ACCWGGT 2 cut(s) 28, 216
SfcI CTRYAG 8 cut(s) 2372, 3147, 3924, 3959, 4253, 4278, 4373, 4799
SfiI GGCCNNNNNGGCC 1 cut(s) 3218
Sfr303I CCGCGG 1 cut(s) 594
SfuI TTCGAA 1 cut(s) 125
SgrBI CCGCGG 1 cut(s) 594
SgsI GGCGCGCC 1 cut(s) 359
SinI GGWCC 4 cut(s) 2116, 3309, 3549, 3664
SmaI CCCGGG 1 cut(s) 5656
SmiMI CAYNNNNRTG 4 cut(s) 14, 875, 899, 2229
SmlI CTYRAG 8 cut(s) 1007, 1277, 1517, 1546, 1608, 2525, 3021, 4085
SmoI CTYRAG 8 cut(s) 1007, 1277, 1517, 1546, 1608, 2525, 3021, 4085
SphI GCATGC 1 cut(s) 1565
SspI AATATT 3 cut(s) 1912, 3477, 4747
SstI GAGCTC 2 cut(s) 1014, 4166
StyI CCWWGG 6 cut(s) 117, 796, 1338, 2430, 2802, 5121
TaiI ACGT 4 cut(s) 147, 687, 2888, 4737
TaqII GACCGA 1 cut(s) 97
TatI WGTACW 4 cut(s) 853, 1858, 2593, 3033
TauI GCSGC 2 cut(s) 319, 593
TseFI GTSAC 6 cut(s) 241, 772, 3060, 3754, 4677, 4987
Tsp45I GTSAC 6 cut(s) 241, 772, 3060, 3754, 4677, 4987
TspGWI ACGGA 2 cut(s) 260, 524
TspMI CCCGGG 1 cut(s) 5654
Tth111I GACNNNGTC 2 cut(s) 143, 3760
Van91I CCANNNNNTGG 1 cut(s) 5702
Vha464I CTTAAG 1 cut(s) 4085
VneI GTGCAC 1 cut(s) 1314
VpaK11BI GGWCC 4 cut(s) 2116, 3309, 3549, 3664
XbaI TCTAGA 2 cut(s) 1054, 1477
XceI RCATGY 3 cut(s) 860, 1565, 2686
XcmI CCANNNNNNNNNTGG 2 cut(s) 3397, 4979
XmaI CCCGGG 1 cut(s) 5654
XmiI GTMKAC 3 cut(s) 1392, 4252, 4968
XmnI GAANNNNTTC 4 cut(s) 1458, 2214, 3888, 5776
ZraI GACGTC 1 cut(s) 145
ZrmI AGTACT 1 cut(s) 1860
Zsp2I ATGCAT 4 cut(s) 1563, 2144, 3412, 5404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.