pycom13g10300

Histone-like transcription factor (CBF/NF-Y) and archaeal histone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0010907
Physical Location & Seq
Reverse (-)
6890407 .. 6891787
1381 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1314 bp
ATGAAAAAACAACAAAAACAAACTTTTGGCGGAAACGATATTTCACATGGCGGGTACAAAACGTTATACCCAAATAGCCATCGATTAAAGTTCCAAAGAACTATGAACTGGTCACGGCGCAGTGGCACACAAAGTAATATAACCTCTAATGCAAGTGCAATACCGTCTATTAAATATGATAAACTGAATGTTTCCCCGTCGCCGCCACCAATGGCAGAAGAAGAGATCGCAGCACGAGAGGAACAGAACCCAGCAGGAGAAGAAGAGAACCCAGCACAAGACGAAGAAGAGAACCCAGCAGACGAAGAAGAGAATCCAGCAGTCGAAGAAGAGAACCCAGCAGGAGAAGAAGACGAAGAAGAGATCCCAGCAGACGAAGAAGAGAATCCAGCAGTCGAAGAAGGGAACCCAGCAGCAGAAGAAGAAAACCTAGCAGGAGAAGAAGAAAGCCCAGCACAAGAAGAAGAAGAGAACCCAGCACAAGAAGAAGAAGAAGAAGAAGAGAACCCAGTACGAGAAAAAGAGAAGGCGGCAAAGGGAAAAGGGCAGGTAGGAGCAGAAGAAGGGAACGGGAATCGGATCCGACCTGAATTCCCAAACGGTCGGGTCAAGAGGATAATGAAGCTGGACAGGGACATCAACAAGATCAACTCGGAAGCCCTACTCCTCGTCTCGTACTCCGCCCAGCTTTTCCTCGAATTCCTGGCGGAGCGGTCAGCGGAGGTCGCGACGGAGAGGAAGCGCAAGGTCGTGAAGCTAGAGCACATGCGAGTCGCGGTCAAGAGGCACCGCCCGACATGCGATTTCCTAATCGATGAGCTTCCCGTGCCCTCTCAGCCGTTCGATCATCCGGCAATCGACCGGAGTCAAGGTCACTCTGTCGCCAGTAGTTCAGCCCCAGCTAAACGAGACCGGAGCCGCACCGCTGCCGCTAGACCAGCCCCAGCGGGCACTCGTCGGATCGATGGTTTCTTTCACAAGCTGACAAAAAAAGCCCCGATTCAGACAGAAGAAACCCCAATTGAGATCGACGAAGCGCCAATCGAGAGCGACGATGCCCCAATTGAGATCGACGAAACACCAATCGAGAGCGACGATGCCCCAATCGAGAGCGACGATGCCCCAATCGAGAACGACGAAGCACCAATCGAGAACGACGAAGCACCAATCGAGAGCGACGATGCCCCAATTGAGAGCGACGATGTCCCAATTGAGGTCGATGATGCCGCAATTGAGAGTGACGAAGCTTCAATGGAGAACGAAGAACCCCTAAATGAGACCCACGGAGCCCCAATTGAGATCCACGAGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 map00230 map00240 map01100 map03020
Pfam Domains
Protein Families
CBFD_NFYB_HMF

Protein Analysis

438

Amino Acids

48.43

Weight (kDa)

4.33

Isoelectric Point (pI)

88.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBFD_NFYB_HMF PF00808 197 - 260 6.3e-17 Histone-like transcription factor (CBF/NF-Y) and archaeal histone
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 538
AccB1I GGYRCC 1 cut(s) 786
AccBSI CCGCTC 1 cut(s) 712
AccII CGCG 2 cut(s) 728, 776
AclI AACGTT 1 cut(s) 62
AclWI GGATC 5 cut(s) 358, 574, 587, 968, 1294
AcsI RAATTY 2 cut(s) 590, 698
AfaI GTAC 3 cut(s) 56, 513, 677
AfiI CCNNNNNNNGG 1 cut(s) 1213
AgsI TTSAA 1 cut(s) 1251
AjnI CCWGG 1 cut(s) 702
AluBI AGCT 7 cut(s) 625, 688, 757, 820, 902, 982, 1247
AluI AGCT 7 cut(s) 625, 688, 757, 820, 902, 982, 1247
Alw21I GWGCWC 1 cut(s) 765
Alw26I GTCTC 3 cut(s) 676, 903, 1271
AlwI GGATC 5 cut(s) 358, 574, 587, 968, 1294
ApeKI GCWGC 3 cut(s) 230, 413, 926
ApoI RAATTY 2 cut(s) 590, 698
AspLEI GCGC 3 cut(s) 120, 744, 1039
BaeGI GKGCMC 2 cut(s) 831, 953
BamHI GGATCC 1 cut(s) 579
BanI GGYRCC 1 cut(s) 786
BanII GRGCYC 1 cut(s) 1291
BauI CACGAG 2 cut(s) 234, 1304
BbsI GAAGAC 1 cut(s) 357
Bbv12I GWGCWC 1 cut(s) 765
BbvI GCAGC 3 cut(s) 242, 425, 913
BccI CCATC 2 cut(s) 87, 959
BceAI ACGGC 2 cut(s) 131, 823
BciT130I CCWGG 1 cut(s) 704
BcoDI GTCTC 3 cut(s) 676, 903, 1271
BfaI CTAG 3 cut(s) 431, 758, 933
BfoI RGCGCY 1 cut(s) 1040
BfuAI ACCTGC 1 cut(s) 538
BisI GCNGC 8 cut(s) 203, 231, 414, 531, 919, 927, 930, 1227
BlsI GCNGC 8 cut(s) 204, 232, 415, 532, 920, 928, 931, 1228
Bme1390I CCNGG 1 cut(s) 704
BmiI GGNNCC 5 cut(s) 407, 581, 788, 917, 1288
BmrFI CCNGG 1 cut(s) 704
BmrI ACTGGG 1 cut(s) 503
BmsI GCATC 5 cut(s) 1045, 1087, 1108, 1171, 1213
BmuI ACTGGG 1 cut(s) 503
BoxI GACNNNNGTC 1 cut(s) 864
BpiI GAAGAC 1 cut(s) 357
Bsa29I ATCGAT 3 cut(s) 82, 813, 963
BsaI GGTCTC 2 cut(s) 903, 1271
BsaJI CCNNGG 1 cut(s) 1282
BsaWI WCCGGW 2 cut(s) 861, 912
BsaXI ACNNNNNCTCC 2 cut(s) 856, 886
Bsc4I CCNNNNNNNGG 1 cut(s) 1213
Bse1I ACTGG 3 cut(s) 113, 509, 885
BseBI CCWGG 1 cut(s) 704
BseCI ATCGAT 3 cut(s) 82, 813, 963
BseDI CCNNGG 1 cut(s) 1282
BseGI GGATG 1 cut(s) 847
BseLI CCNNNNNNNGG 1 cut(s) 1213
BseMII CTCAG 1 cut(s) 848
BseNI ACTGG 3 cut(s) 113, 509, 885
BseRI GAGGAG 1 cut(s) 656
BseSI GKGCMC 2 cut(s) 831, 953
BseXI GCAGC 3 cut(s) 242, 425, 913
Bsh1236I CGCG 2 cut(s) 728, 776
Bsh1285I CGRYCG 2 cut(s) 604, 862
BshNI GGYRCC 1 cut(s) 786
BshVI ATCGAT 3 cut(s) 82, 813, 963
BsiEI CGRYCG 2 cut(s) 604, 862
BsiHKAI GWGCWC 1 cut(s) 765
BsiSI CCGG 3 cut(s) 851, 862, 913
BslFI GGGAC 2 cut(s) 647, 1190
BslI CCNNNNNNNGG 1 cut(s) 1213
BsmAI GTCTC 3 cut(s) 676, 903, 1271
BsmBI CGTCTC 1 cut(s) 676
BsmFI GGGAC 2 cut(s) 647, 1190
Bso31I GGTCTC 2 cut(s) 903, 1271
Bsp1286I GDGCHC 4 cut(s) 765, 831, 953, 1291
Bsp143I GATC 9 cut(s) 225, 363, 579, 645, 844, 960, 1026, 1068, 1299
Bsp68I TCGCGA 1 cut(s) 728
BspCNI CTCAG 1 cut(s) 847
BspDI ATCGAT 3 cut(s) 82, 813, 963
BspFNI CGCG 2 cut(s) 728, 776
BspLI GGNNCC 5 cut(s) 407, 581, 788, 917, 1288
BspMI ACCTGC 1 cut(s) 538
BspPI GGATC 5 cut(s) 358, 574, 587, 968, 1294
BspT107I GGYRCC 1 cut(s) 786
BspTNI GGTCTC 2 cut(s) 903, 1271
BsrBI CCGCTC 1 cut(s) 712
BsrI ACTGG 3 cut(s) 113, 509, 885
BssECI CCNNGG 1 cut(s) 1282
BssMI GATC 9 cut(s) 225, 363, 579, 645, 844, 960, 1026, 1068, 1299
BssSI CACGAG 2 cut(s) 234, 1304
Bst2BI CACGAG 2 cut(s) 234, 1304
Bst2UI CCWGG 1 cut(s) 704
Bst4CI ACNGT 2 cut(s) 165, 602
Bst6I CTCTTC 9 cut(s) 216, 258, 282, 303, 324, 354, 375, 462, 495
BstC8I GCNNGC 1 cut(s) 949
BstDEI CTNAG 1 cut(s) 834
BstDSI CCRYGG 1 cut(s) 1282
BstF5I GGATG 1 cut(s) 847
BstFNI CGCG 2 cut(s) 728, 776
BstH2I RGCGCY 1 cut(s) 1040
BstHHI GCGC 3 cut(s) 120, 744, 1039
BstKTI GATC 9 cut(s) 228, 366, 582, 648, 847, 963, 1029, 1071, 1302
BstMAI GTCTC 3 cut(s) 676, 903, 1271
BstMBI GATC 9 cut(s) 225, 363, 579, 645, 844, 960, 1026, 1068, 1299
BstMCI CGRYCG 2 cut(s) 604, 862
BstMWI GCNNNNNNNGC 5 cut(s) 725, 798, 826, 835, 938
BstNI CCWGG 1 cut(s) 704
BstNSI RCATGY 2 cut(s) 769, 801
BstPAI GACNNNNGTC 1 cut(s) 864
BstSCI CCNGG 1 cut(s) 702
BstSLI GKGCMC 2 cut(s) 831, 953
BstUI CGCG 2 cut(s) 728, 776
BstV1I GCAGC 3 cut(s) 242, 425, 913
BstV2I GAAGAC 1 cut(s) 357
BstX2I RGATCY 3 cut(s) 363, 579, 1299
BstYI RGATCY 3 cut(s) 363, 579, 1299
Bsu15I ATCGAT 3 cut(s) 82, 813, 963
BsuTUI ATCGAT 3 cut(s) 82, 813, 963
BtgI CCRYGG 1 cut(s) 1282
BtsCI GGATG 1 cut(s) 847
BtsI GCAGTG 1 cut(s) 127
BtsIMutI CAGTG 1 cut(s) 127
BtuMI TCGCGA 1 cut(s) 728
BveI ACCTGC 1 cut(s) 538
Cac8I GCNNGC 1 cut(s) 949
CfoI GCGC 3 cut(s) 120, 744, 1039
ClaI ATCGAT 3 cut(s) 82, 813, 963
Csp6I GTAC 3 cut(s) 55, 512, 676
CviAII CATG 3 cut(s) 47, 766, 798
CviQI GTAC 3 cut(s) 55, 512, 676
DdeI CTNAG 1 cut(s) 834
DpnI GATC 9 cut(s) 227, 365, 581, 647, 846, 962, 1028, 1070, 1301
DpnII GATC 9 cut(s) 225, 363, 579, 645, 844, 960, 1026, 1068, 1299
Eam1104I CTCTTC 9 cut(s) 216, 258, 282, 303, 324, 354, 375, 462, 495
EarI CTCTTC 9 cut(s) 216, 258, 282, 303, 324, 354, 375, 462, 495
EciI GGCGGA 3 cut(s) 45, 670, 722
Eco24I GRGCYC 1 cut(s) 1291
Eco31I GGTCTC 2 cut(s) 903, 1271
EcoRI GAATTC 2 cut(s) 590, 698
EcoRII CCWGG 1 cut(s) 702
EcoT38I GRGCYC 1 cut(s) 1291
Esp3I CGTCTC 1 cut(s) 676
FaeI CATG 3 cut(s) 50, 769, 801
FaiI YATR 8 cut(s) 48, 67, 104, 140, 177, 767, 799, 1312
FaqI GGGAC 2 cut(s) 647, 1190
FatI CATG 3 cut(s) 46, 765, 797
FauI CCCGC 2 cut(s) 44, 940
Fnu4HI GCNGC 8 cut(s) 203, 231, 414, 531, 919, 927, 930, 1227
FokI GGATG 1 cut(s) 834
FriOI GRGCYC 1 cut(s) 1291
Fsp4HI GCNGC 8 cut(s) 203, 231, 414, 531, 919, 927, 930, 1227
FspBI CTAG 3 cut(s) 431, 758, 933
GlaI GCGC 3 cut(s) 119, 743, 1038
GluI GCNGC 8 cut(s) 203, 231, 414, 531, 919, 927, 930, 1227
HaeII RGCGCY 1 cut(s) 1040
HapII CCGG 3 cut(s) 851, 862, 913
HhaI GCGC 3 cut(s) 120, 744, 1039
Hin1II CATG 3 cut(s) 50, 769, 801
Hin6I GCGC 3 cut(s) 118, 742, 1037
HinP1I GCGC 3 cut(s) 118, 742, 1037
HindIII AAGCTT 1 cut(s) 1245
HinfI GANTC 7 cut(s) 313, 385, 574, 771, 865, 1000, 1307
HpaII CCGG 3 cut(s) 851, 862, 913
Hpy188I TCNGA 5 cut(s) 579, 584, 655, 960, 1005
HpyAV CCTTC 3 cut(s) 395, 520, 557
HpyCH4III ACNGT 2 cut(s) 165, 602
HpyCH4IV ACGT 1 cut(s) 62
HpyCH4V TGCA 2 cut(s) 152, 158
HpyF10VI GCNNNNNNNGC 5 cut(s) 725, 798, 826, 835, 938
HpyF3I CTNAG 1 cut(s) 834
HpySE526I ACGT 1 cut(s) 62
Hsp92II CATG 3 cut(s) 50, 769, 801
HspAI GCGC 3 cut(s) 118, 742, 1037
Kzo9I GATC 9 cut(s) 225, 363, 579, 645, 844, 960, 1026, 1068, 1299
LmnI GCTCC 4 cut(s) 554, 709, 915, 1286
Lsp1109I GCAGC 3 cut(s) 242, 425, 913
LweI GCATC 5 cut(s) 1045, 1087, 1108, 1171, 1213
MaeI CTAG 3 cut(s) 431, 758, 933
MaeII ACGT 1 cut(s) 62
MaeIII GTNAC 3 cut(s) 111, 872, 1238
MalI GATC 9 cut(s) 227, 365, 581, 647, 846, 962, 1028, 1070, 1301
MbiI CCGCTC 1 cut(s) 712
MboI GATC 9 cut(s) 225, 363, 579, 645, 844, 960, 1026, 1068, 1299
MfeI CAATTG 6 cut(s) 1020, 1062, 1188, 1209, 1230, 1293
MflI RGATCY 3 cut(s) 363, 579, 1299
MhlI GDGCHC 4 cut(s) 765, 831, 953, 1291
MluCI AATT 8 cut(s) 590, 698, 1020, 1062, 1188, 1209, 1230, 1293
MlyI GAGTC 2 cut(s) 780, 874
MmeI TCCRAC 2 cut(s) 607, 938
MseI TTAA 2 cut(s) 86, 171
MspA1I CMGCKG 3 cut(s) 719, 926, 947
MspI CCGG 3 cut(s) 851, 862, 913
MspR9I CCNGG 1 cut(s) 704
MunI CAATTG 6 cut(s) 1020, 1062, 1188, 1209, 1230, 1293
MvaI CCWGG 1 cut(s) 704
MvnI CGCG 2 cut(s) 728, 776
MwoI GCNNNNNNNGC 5 cut(s) 725, 798, 826, 835, 938
NdeII GATC 9 cut(s) 225, 363, 579, 645, 844, 960, 1026, 1068, 1299
NlaIII CATG 3 cut(s) 50, 769, 801
NlaIV GGNNCC 5 cut(s) 407, 581, 788, 917, 1288
NmuCI GTSAC 3 cut(s) 111, 872, 1238
NruI TCGCGA 1 cut(s) 728
NspI RCATGY 2 cut(s) 769, 801
PcsI WCGNNNNNNNCGW 6 cut(s) 1050, 1092, 1113, 1134, 1155, 1176
PfeI GAWTC 4 cut(s) 313, 385, 574, 1000
PflFI GACNNNGTC 1 cut(s) 1202
PkrI GCNGC 8 cut(s) 204, 232, 415, 532, 920, 928, 931, 1228
PleI GAGTC 2 cut(s) 779, 873
PpsI GAGTC 2 cut(s) 779, 873
PshAI GACNNNNGTC 1 cut(s) 864
Psp1406I AACGTT 1 cut(s) 62
Psp6I CCWGG 1 cut(s) 702
PspGI CCWGG 1 cut(s) 702
PspN4I GGNNCC 5 cut(s) 407, 581, 788, 917, 1288
PsuI RGATCY 3 cut(s) 363, 579, 1299
PsyI GACNNNGTC 1 cut(s) 1202
RruI TCGCGA 1 cut(s) 728
RsaI GTAC 3 cut(s) 56, 513, 677
RsaNI GTAC 3 cut(s) 55, 512, 676
SaqAI TTAA 2 cut(s) 86, 171
SatI GCNGC 8 cut(s) 203, 231, 414, 531, 919, 927, 930, 1227
Sau3AI GATC 9 cut(s) 225, 363, 579, 645, 844, 960, 1026, 1068, 1299
SchI GAGTC 2 cut(s) 780, 874
ScrFI CCNGG 1 cut(s) 704
SduI GDGCHC 4 cut(s) 765, 831, 953, 1291
SfaNI GCATC 5 cut(s) 1045, 1087, 1108, 1171, 1213
Sse9I AATT 8 cut(s) 590, 698, 1020, 1062, 1188, 1209, 1230, 1293
SspMI CTAG 3 cut(s) 431, 758, 933
StyD4I CCNGG 1 cut(s) 702
TaaI ACNGT 2 cut(s) 165, 602
TaiI ACGT 1 cut(s) 65
TasI AATT 8 cut(s) 590, 698, 1020, 1062, 1188, 1209, 1230, 1293
TauI GCSGC 5 cut(s) 205, 533, 921, 932, 1229
TfiI GAWTC 4 cut(s) 313, 385, 574, 1000
Tru1I TTAA 2 cut(s) 86, 171
Tru9I TTAA 2 cut(s) 86, 171
TscAI CASTG 1 cut(s) 127
TseFI GTSAC 3 cut(s) 111, 872, 1238
TseI GCWGC 3 cut(s) 230, 413, 926
Tsp45I GTSAC 3 cut(s) 111, 872, 1238
TspDTI ATGAA 3 cut(s) 17, 119, 635
TspGWI ACGGA 2 cut(s) 746, 1299
TspRI CASTG 1 cut(s) 127
Tth111I GACNNNGTC 1 cut(s) 1202
XapI RAATTY 2 cut(s) 590, 698
XceI RCATGY 2 cut(s) 769, 801
XspI CTAG 3 cut(s) 431, 758, 933
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.