pycom13g14260

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0009113
Physical Location & Seq
Forward (+)
10261506 .. 10262795
1290 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g14260.1

Sequence Viewer

Length: 1167 bp
ATGAATGCTGTGGCTGATCAAGATCAAGACATGGGAGATGGAATGCAGTGCAGTGACCATCCATACAGAAGCAACCCAGGTGGGATCTGCGCTTTCTGCCTCCAGGAGAAGCTCGGCAAGCTTGTTTCTTCCTCCTTCCCTCTCCCCATCCACGCTCCTGCAACCTCCTCTTCTTCCTCCCCTTCTTTTAGATCTGACATTATTAACGGCGGCAATGGAGCTGCTGCTGCTGCTGCTGCTGGTGGTCCTTCTTCCTCCGCCGCCTCCCTCGCCCTCTCCCTATCCAAAGTGAGTGACGGTGCTGCTACTACTGGTACCGGTGGTGCTACTGTTGTATCTTCCGATAGGGAAGCTGCTAACATGGTGTTCAAGCGGAGCAAGTCTACGACCAACCCGCGGCGGGGTCACCATTTTCTGGATGGTTCAGAGGATTTTAGTCCAAGGAAGAGAGGCGGGTTCTGGTCGTTTCTGTATCACTCCTCCACCAAGAACTCGTCACACGCTCTTAACAAGAAGACAATGGACAAGTCCAGCTTCAGAGACAACTCCACCTCCAAGATCTCTTCCTCTTCTTCCTTCACATCCGCCCAAAAGGACTCCAAAAGCTTCGGGTCTTCTTCTCTGAGGAATAGAAACGAACTAGCGGTCGACGCGGACGACGACAGCAACAGCCCAAACAGCAGTCAAGCAACCGCCGCCTCGGCCTCCTCCTTCGAGCGGAAGGTCTCGAGATCCAGATCCGTCGGCTGCGGAAGCCGAAGCTTCTCCGGCGACTTCTTCGAGCGAATCTCAACTGGGTTCGGCGACTGCACTCTCAGAAGAGTCGAGTCGCAGAGAGAAGGCAAGCCCAAAGCAGCCCACCGCGGTGGCGAACACAACCACTGCATGAAAGAGCGAGTGAAATGCGGCGGGATTTTCAGTGGTTTCATGATGACGTCCTCGTCTTCGTCATCGTCGTCGTCGTCTTACTGGGTTTCGTCCTCCGCCGAAGGGCCGCTCGTCAACGGCCGTAACCGGAGCTGGGGGTGGGCGTTTGCGAGTCCGATGAGAGCATTCACCAAACCTTCTTCTAAAGATGGGAAGAGGGACATAGTTAGACAAGCTTCAGATAAGAACACAGCTCCAAACTTGAATGCGATTCCTTCCTTGCTATCAGTCAATAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

389

Amino Acids

40.88

Weight (kDa)

9.68

Isoelectric Point (pI)

51.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 940
AatII GACGTC 1 cut(s) 938
Acc65I GGTACC 1 cut(s) 314
AccB1I GGYRCC 1 cut(s) 314
AccB7I CCANNNNNTGG 1 cut(s) 415
AccBSI CCGCTC 2 cut(s) 718, 997
AccI GTMKAC 2 cut(s) 383, 648
AccII CGCG 3 cut(s) 397, 653, 864
AclWI GGATC 3 cut(s) 92, 726, 732
AcoI YGGCCR 1 cut(s) 1006
AcuI CTGAAG 2 cut(s) 520, 1089
AcyI GRCGYC 1 cut(s) 935
AfaI GTAC 1 cut(s) 316
AfiI CCNNNNNNNGG 7 cut(s) 396, 400, 401, 415, 717, 990, 1021
AgeI ACCGGT 1 cut(s) 317
AgsI TTSAA 2 cut(s) 370, 1132
AjnI CCWGG 2 cut(s) 76, 102
AleI CACNNNNGTG 1 cut(s) 864
Alw26I GTCTC 2 cut(s) 534, 730
AlwI GGATC 3 cut(s) 92, 726, 732
Ama87I CYCGRG 1 cut(s) 727
AoxI GGCC 3 cut(s) 702, 992, 1006
ArsI GACNNNNNNTTYG 2 cut(s) 630, 662
AsiGI ACCGGT 1 cut(s) 317
Asp718I GGTACC 1 cut(s) 314
AspLEI GCGC 1 cut(s) 92
AspS9I GGNCC 2 cut(s) 245, 992
AsuHPI GGTGA 2 cut(s) 398, 1048
AvaI CYCGRG 1 cut(s) 727
AvaII GGWCC 1 cut(s) 245
BaeI ACNNNNGTAYC 2 cut(s) 318, 351
BanI GGYRCC 1 cut(s) 314
BbsI GAAGAC 3 cut(s) 521, 606, 936
BccI CCATC 5 cut(s) 32, 66, 155, 413, 1070
BceAI ACGGC 3 cut(s) 223, 993, 1021
BcgI CGANNNNNNTGC 2 cut(s) 885, 919
BciT130I CCWGG 2 cut(s) 78, 104
BclI TGATCA 1 cut(s) 16
BcoDI GTCTC 2 cut(s) 534, 730
BfaI CTAG 1 cut(s) 641
BglI GCCNNNNNGGC 1 cut(s) 701
BglII AGATCT 2 cut(s) 191, 558
Bme1390I CCNGG 2 cut(s) 78, 104
Bme18I GGWCC 1 cut(s) 245
BmeT110I CYCGRG 1 cut(s) 727
BmgT120I GGNCC 2 cut(s) 245, 992
BmiI GGNNCC 1 cut(s) 316
BmrFI CCNGG 2 cut(s) 78, 104
BmrI ACTGGG 2 cut(s) 804, 979
BmuI ACTGGG 2 cut(s) 804, 979
BpiI GAAGAC 3 cut(s) 521, 606, 936
BplI GAGNNNNNCTC 2 cut(s) 773, 805
BpmI CTGGAG 1 cut(s) 86
BsaBI GATNNNNATC 2 cut(s) 21, 736
BsaHI GRCGYC 1 cut(s) 935
BsaI GGTCTC 1 cut(s) 730
BsaJI CCNNGG 5 cut(s) 76, 395, 440, 699, 862
BsaWI WCCGGW 2 cut(s) 317, 1014
BsaXI ACNNNNNCTCC 2 cut(s) 536, 566
Bsc4I CCNNNNNNNGG 7 cut(s) 396, 400, 401, 415, 717, 990, 1021
Bse118I RCCGGY 1 cut(s) 317
Bse1I ACTGG 3 cut(s) 316, 799, 974
Bse3DI GCAATG 1 cut(s) 220
Bse8I GATNNNNATC 2 cut(s) 21, 736
BseBI CCWGG 2 cut(s) 78, 104
BseDI CCNNGG 5 cut(s) 76, 395, 440, 699, 862
BseGI GGATG 4 cut(s) 58, 147, 424, 581
BseJI GATNNNNATC 2 cut(s) 21, 736
BseLI CCNNNNNNNGG 7 cut(s) 396, 400, 401, 415, 717, 990, 1021
BseMI GCAATG 1 cut(s) 220
BseMII CTCAG 2 cut(s) 614, 829
BseNI ACTGG 3 cut(s) 316, 799, 974
BseRI GAGGAG 3 cut(s) 157, 469, 697
BseX3I CGGCCG 1 cut(s) 1006
BseYI CCCAGC 1 cut(s) 1020
BsgI GTGCAG 2 cut(s) 70, 793
Bsh1236I CGCG 3 cut(s) 397, 653, 864
Bsh1285I CGRYCG 2 cut(s) 648, 1009
BshFI GGCC 3 cut(s) 704, 994, 1008
BshNI GGYRCC 1 cut(s) 314
BshTI ACCGGT 1 cut(s) 317
BsiEI CGRYCG 2 cut(s) 648, 1009
BsiHKCI CYCGRG 1 cut(s) 727
BsiSI CCGG 3 cut(s) 318, 768, 1015
BslFI GGGAC 1 cut(s) 1100
BslI CCNNNNNNNGG 7 cut(s) 396, 400, 401, 415, 717, 990, 1021
BsmAI GTCTC 2 cut(s) 534, 730
BsmFI GGGAC 1 cut(s) 1100
BsmI GAATGC 4 cut(s) 10, 48, 1052, 1138
BsnI GGCC 3 cut(s) 704, 994, 1008
Bso31I GGTCTC 1 cut(s) 730
BsoBI CYCGRG 1 cut(s) 727
Bsp143I GATC 7 cut(s) 16, 22, 84, 191, 558, 731, 737
BspANI GGCC 3 cut(s) 704, 994, 1008
BspCNI CTCAG 2 cut(s) 615, 828
BspFNI CGCG 3 cut(s) 397, 653, 864
BspHI TCATGA 1 cut(s) 927
BspLI GGNNCC 1 cut(s) 316
BspPI GGATC 3 cut(s) 92, 726, 732
BspT107I GGYRCC 1 cut(s) 314
BspTNI GGTCTC 1 cut(s) 730
BsrBI CCGCTC 2 cut(s) 718, 997
BsrDI GCAATG 1 cut(s) 220
BsrFI RCCGGY 1 cut(s) 317
BsrI ACTGG 3 cut(s) 316, 799, 974
BssAI RCCGGY 1 cut(s) 317
BssECI CCNNGG 5 cut(s) 76, 395, 440, 699, 862
BssMI GATC 7 cut(s) 16, 22, 84, 191, 558, 731, 737
BssNI GRCGYC 1 cut(s) 935
BssT1I CCWWGG 1 cut(s) 440
Bst2UI CCWGG 2 cut(s) 78, 104
Bst4CI ACNGT 2 cut(s) 299, 331
Bst6I CTCTTC 6 cut(s) 175, 440, 568, 574, 814, 1076
BstACI GRCGYC 1 cut(s) 935
BstC8I GCNNGC 2 cut(s) 119, 845
BstDEI CTNAG 2 cut(s) 623, 815
BstDSI CCRYGG 2 cut(s) 395, 862
BstEII GGTNACC 1 cut(s) 404
BstF5I GGATG 4 cut(s) 58, 147, 424, 581
BstFNI CGCG 3 cut(s) 397, 653, 864
BstHHI GCGC 1 cut(s) 92
BstKTI GATC 7 cut(s) 19, 25, 87, 194, 561, 734, 740
BstMAI GTCTC 2 cut(s) 534, 730
BstMBI GATC 7 cut(s) 16, 22, 84, 191, 558, 731, 737
BstMCI CGRYCG 2 cut(s) 648, 1009
BstNI CCWGG 2 cut(s) 78, 104
BstPI GGTNACC 1 cut(s) 404
BstSCI CCNGG 2 cut(s) 76, 102
BstUI CGCG 3 cut(s) 397, 653, 864
BstV2I GAAGAC 3 cut(s) 521, 606, 936
BstX2I RGATCY 5 cut(s) 84, 191, 558, 731, 737
BstXI CCANNNNNNTGG 1 cut(s) 866
BstYI RGATCY 5 cut(s) 84, 191, 558, 731, 737
BstZI CGGCCG 1 cut(s) 1006
BsuRI GGCC 3 cut(s) 704, 994, 1008
BtgI CCRYGG 2 cut(s) 395, 862
BtsCI GGATG 4 cut(s) 58, 147, 424, 581
BtsI GCAGTG 3 cut(s) 53, 58, 880
BtsIMutI CAGTG 4 cut(s) 53, 58, 880, 925
Cac8I GCNNGC 2 cut(s) 119, 845
CciI TCATGA 1 cut(s) 927
CfoI GCGC 1 cut(s) 92
Cfr10I RCCGGY 1 cut(s) 317
Cfr13I GGNCC 2 cut(s) 245, 992
Cfr42I CCGCGG 2 cut(s) 398, 865
CseI GACGC 1 cut(s) 659
Csp6I GTAC 1 cut(s) 315
CspAI ACCGGT 1 cut(s) 317
CspCI CAANNNNNGTGG 2 cut(s) 61, 96
CviAII CATG 4 cut(s) 31, 361, 886, 928
CviQI GTAC 1 cut(s) 315
DdeI CTNAG 2 cut(s) 623, 815
DpnI GATC 7 cut(s) 18, 24, 86, 193, 560, 733, 739
DpnII GATC 7 cut(s) 16, 22, 84, 191, 558, 731, 737
DrdI GACNNNNNNGTC 1 cut(s) 940
DseDI GACNNNNNNGTC 1 cut(s) 940
EaeI YGGCCR 1 cut(s) 1006
EagI CGGCCG 1 cut(s) 1006
Eam1104I CTCTTC 6 cut(s) 175, 440, 568, 574, 814, 1076
EarI CTCTTC 6 cut(s) 175, 440, 568, 574, 814, 1076
EciI GGCGGA 3 cut(s) 247, 574, 973
EclXI CGGCCG 1 cut(s) 1006
Eco130I CCWWGG 1 cut(s) 440
Eco31I GGTCTC 1 cut(s) 730
Eco47I GGWCC 1 cut(s) 245
Eco52I CGGCCG 1 cut(s) 1006
Eco57I CTGAAG 2 cut(s) 520, 1089
Eco88I CYCGRG 1 cut(s) 727
Eco91I GGTNACC 1 cut(s) 404
EcoO65I GGTNACC 1 cut(s) 404
EcoRII CCWGG 2 cut(s) 76, 102
EcoT14I CCWWGG 1 cut(s) 440
ErhI CCWWGG 1 cut(s) 440
FaeI CATG 4 cut(s) 34, 364, 889, 931
FaiI YATR 6 cut(s) 32, 64, 362, 887, 929, 1091
FalI AAGNNNNNCTT 2 cut(s) 518, 550
FaqI GGGAC 1 cut(s) 1100
FatI CATG 4 cut(s) 30, 360, 885, 927
FauI CCCGC 4 cut(s) 393, 402, 446, 902
FbaI TGATCA 1 cut(s) 16
FblI GTMKAC 2 cut(s) 383, 648
FokI GGATG 4 cut(s) 45, 134, 431, 568
FspBI CTAG 1 cut(s) 641
GlaI GCGC 1 cut(s) 91
GsaI CCCAGC 1 cut(s) 1024
GsuI CTGGAG 1 cut(s) 86
HaeIII GGCC 3 cut(s) 704, 994, 1008
HapII CCGG 3 cut(s) 318, 768, 1015
HgaI GACGC 1 cut(s) 659
HhaI GCGC 1 cut(s) 92
Hin1I GRCGYC 1 cut(s) 935
Hin1II CATG 4 cut(s) 34, 364, 889, 931
Hin6I GCGC 1 cut(s) 90
HinP1I GCGC 1 cut(s) 90
HincII GTYRAC 2 cut(s) 649, 1003
HindII GTYRAC 2 cut(s) 649, 1003
HindIII AAGCTT 4 cut(s) 119, 604, 760, 1101
HinfI GANTC 6 cut(s) 596, 786, 822, 827, 1039, 1138
HpaII CCGG 3 cut(s) 318, 768, 1015
HphI GGTGA 2 cut(s) 398, 1048
Hpy166II GTNNAC 3 cut(s) 384, 649, 1003
Hpy188I TCNGA 8 cut(s) 196, 343, 427, 539, 624, 818, 1044, 1108
Hpy188III TCNNGA 7 cut(s) 20, 26, 416, 727, 729, 735, 928
Hpy8I GTNNAC 3 cut(s) 384, 649, 1003
Hpy99I CGWCG 6 cut(s) 653, 662, 746, 958, 961, 964
HpyCH4III ACNGT 2 cut(s) 299, 331
HpyCH4IV ACGT 1 cut(s) 935
HpyCH4V TGCA 5 cut(s) 46, 51, 161, 810, 885
HpyF3I CTNAG 2 cut(s) 623, 815
HpySE526I ACGT 1 cut(s) 935
Hsp92I GRCGYC 1 cut(s) 935
Hsp92II CATG 4 cut(s) 34, 364, 889, 931
HspAI GCGC 1 cut(s) 90
KpnI GGTACC 1 cut(s) 318
Ksp22I TGATCA 1 cut(s) 16
KspI CCGCGG 2 cut(s) 398, 865
Kzo9I GATC 7 cut(s) 16, 22, 84, 191, 558, 731, 737
LmnI GCTCC 5 cut(s) 160, 218, 375, 1017, 1126
MaeI CTAG 1 cut(s) 641
MaeII ACGT 1 cut(s) 935
MaeIII GTNAC 5 cut(s) 53, 293, 404, 495, 1010
MalI GATC 7 cut(s) 18, 24, 86, 193, 560, 733, 739
MbiI CCGCTC 2 cut(s) 718, 997
MboI GATC 7 cut(s) 16, 22, 84, 191, 558, 731, 737
MflI RGATCY 5 cut(s) 84, 191, 558, 731, 737
MlyI GAGTC 4 cut(s) 590, 831, 836, 1048
MseI TTAA 2 cut(s) 204, 507
MslI CAYNNNNRTG 1 cut(s) 864
MspA1I CMGCKG 2 cut(s) 397, 864
MspI CCGG 3 cut(s) 318, 768, 1015
MspR9I CCNGG 2 cut(s) 78, 104
Mva1269I GAATGC 4 cut(s) 10, 48, 1052, 1138
MvaI CCWGG 2 cut(s) 78, 104
MvnI CGCG 3 cut(s) 397, 653, 864
NdeII GATC 7 cut(s) 16, 22, 84, 191, 558, 731, 737
NlaIII CATG 4 cut(s) 34, 364, 889, 931
NlaIV GGNNCC 1 cut(s) 316
NmeAIII GCCGAG 2 cut(s) 93, 680
NmuCI GTSAC 4 cut(s) 53, 293, 404, 495
OliI CACNNNNGTG 1 cut(s) 864
PaeR7I CTCGAG 1 cut(s) 727
PagI TCATGA 1 cut(s) 927
PcsI WCGNNNNNNNCGW 4 cut(s) 654, 657, 953, 959
PctI GAATGC 4 cut(s) 10, 48, 1052, 1138
PfeI GAWTC 2 cut(s) 786, 1138
PflMI CCANNNNNTGG 1 cut(s) 415
PfoI TCCNGGA 1 cut(s) 102
PinAI ACCGGT 1 cut(s) 317
PleI GAGTC 4 cut(s) 590, 830, 835, 1047
PpsI GAGTC 4 cut(s) 590, 830, 835, 1047
Psp6I CCWGG 2 cut(s) 76, 102
PspEI GGTNACC 1 cut(s) 404
PspFI CCCAGC 1 cut(s) 1020
PspGI CCWGG 2 cut(s) 76, 102
PspN4I GGNNCC 1 cut(s) 316
PspPI GGNCC 2 cut(s) 245, 992
PsuI RGATCY 5 cut(s) 84, 191, 558, 731, 737
RsaI GTAC 1 cut(s) 316
RsaNI GTAC 1 cut(s) 315
RseI CAYNNNNRTG 1 cut(s) 864
SacII CCGCGG 2 cut(s) 398, 865
SalI GTCGAC 1 cut(s) 647
SaqAI TTAA 2 cut(s) 204, 507
Sau3AI GATC 7 cut(s) 16, 22, 84, 191, 558, 731, 737
Sau96I GGNCC 2 cut(s) 245, 992
SchI GAGTC 4 cut(s) 590, 831, 836, 1048
ScrFI CCNGG 2 cut(s) 78, 104
Sfr274I CTCGAG 1 cut(s) 727
Sfr303I CCGCGG 2 cut(s) 398, 865
SgrBI CCGCGG 2 cut(s) 398, 865
SinI GGWCC 1 cut(s) 245
SlaI CTCGAG 1 cut(s) 727
SmiMI CAYNNNNRTG 1 cut(s) 864
SmlI CTYRAG 1 cut(s) 727
SmoI CTYRAG 1 cut(s) 727
SspMI CTAG 1 cut(s) 641
StyD4I CCNGG 2 cut(s) 76, 102
StyI CCWWGG 1 cut(s) 440
TaaI ACNGT 2 cut(s) 299, 331
TaiI ACGT 1 cut(s) 938
TaqI TCGA 5 cut(s) 648, 714, 728, 780, 825
TauI GCSGC 6 cut(s) 213, 263, 400, 698, 909, 997
TfiI GAWTC 2 cut(s) 786, 1138
Tru1I TTAA 2 cut(s) 204, 507
Tru9I TTAA 2 cut(s) 204, 507
TscAI CASTG 4 cut(s) 53, 58, 887, 925
TseFI GTSAC 4 cut(s) 53, 293, 404, 495
Tsp45I GTSAC 4 cut(s) 53, 293, 404, 495
TspDTI ATGAA 3 cut(s) 17, 902, 916
TspGWI ACGGA 1 cut(s) 730
TspRI CASTG 4 cut(s) 53, 58, 887, 925
Van91I CCANNNNNTGG 1 cut(s) 415
VpaK11BI GGWCC 1 cut(s) 245
XcmI CCANNNNNNNNNTGG 1 cut(s) 416
XhoI CTCGAG 1 cut(s) 727
XmiI GTMKAC 2 cut(s) 383, 648
XspI CTAG 1 cut(s) 641
ZraI GACGTC 1 cut(s) 936
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.