pycom13g18330

HR-like lesion-inducing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0009048
Physical Location & Seq
Forward (+)
14396271 .. 14398035
1765 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 480 bp
ATGGCTTTCGTTTCGTTCGTTGGACGCCTTCTTTTCGCCTCTGTCTTCTTCCTCTCTGCTTGGCATGAGTTCAATGAATTTGGCGTGGACGGGGGACCCGCAGCCGAATATCTGAGACCAAAATTCGACGTTTTCTCGAATCATCTGTCAACTCACACTGGTGTGCAAGTGCCCAAACAAGTTGAAATCAAACATTTAGTGGCAGCCATGGTGGCATTGAAGGGCATTGGGGGTCTTCTCTTCATCTTTGGTTCTTCTATTGGCGCCTATTTGCTGCTTCTGCATCAGGCCATTGCTACTCCTATCTTGTATGATTTCTACAACTATGATCCCGAGAAGACAGAATTCAATCAACTCTTTTTTCAGTTTACTCAGAACATGGCATTCTTCGGGGCTCTGCTCTTTTTCGTAGGCATGAAGACATTGATACCAAAGAGACAACAACTCAGGAAAAGAGCTCCAAAACCAAAAACTACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HR_lesion

Protein Analysis

160

Amino Acids

17.97

Weight (kDa)

9.56

Isoelectric Point (pI)

31.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HR_lesion PF05514 1 - 139 1.4e-63 HR-like lesion-inducing
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 263
AciI CCGC 1 cut(s) 99
AclWI GGATC 1 cut(s) 323
AcsI RAATTY 3 cut(s) 77, 122, 344
AcyI GRCGYC 2 cut(s) 25, 264
AgsI TTSAA 4 cut(s) 73, 185, 220, 349
AleI CACNNNNGTG 2 cut(s) 159, 161
AluBI AGCT 1 cut(s) 458
AluI AGCT 1 cut(s) 458
Alw21I GWGCWC 1 cut(s) 460
Alw26I GTCTC 2 cut(s) 109, 430
AlwI GGATC 1 cut(s) 323
Ama87I CYCGRG 1 cut(s) 332
AoxI GGCC 1 cut(s) 288
ApeKI GCWGC 3 cut(s) 101, 203, 274
ApoI RAATTY 3 cut(s) 77, 122, 344
ArsI GACNNNNNNTTYG 2 cut(s) 131, 163
AspLEI GCGC 1 cut(s) 266
AspS9I GGNCC 1 cut(s) 95
AvaI CYCGRG 1 cut(s) 332
AvaII GGWCC 1 cut(s) 95
BaeGI GKGCMC 1 cut(s) 174
BanI GGYRCC 1 cut(s) 263
BanII GRGCYC 2 cut(s) 397, 460
BbsI GAAGAC 4 cut(s) 37, 227, 344, 425
Bbv12I GWGCWC 1 cut(s) 460
BbvI GCAGC 3 cut(s) 113, 215, 261
BcoDI GTCTC 2 cut(s) 109, 430
BfoI RGCGCY 1 cut(s) 267
BglI GCCNNNNNGGC 1 cut(s) 212
BisI GCNGC 3 cut(s) 102, 204, 275
BlsI GCNGC 3 cut(s) 103, 205, 276
Bme18I GGWCC 1 cut(s) 95
BmeT110I CYCGRG 1 cut(s) 332
BmgT120I GGNCC 1 cut(s) 95
BmiI GGNNCC 3 cut(s) 96, 97, 265
BmsI GCATC 1 cut(s) 292
BpiI GAAGAC 4 cut(s) 37, 227, 344, 425
BsaHI GRCGYC 2 cut(s) 25, 264
BsaI GGTCTC 1 cut(s) 109
BsaJI CCNNGG 1 cut(s) 207
Bse1I ACTGG 1 cut(s) 163
Bse3DI GCAATG 1 cut(s) 291
BseDI CCNNGG 1 cut(s) 207
BseMI GCAATG 1 cut(s) 291
BseMII CTCAG 3 cut(s) 104, 386, 460
BseNI ACTGG 1 cut(s) 163
BseSI GKGCMC 1 cut(s) 174
BseXI GCAGC 3 cut(s) 113, 215, 261
BshFI GGCC 1 cut(s) 290
BshNI GGYRCC 1 cut(s) 263
BsiHKAI GWGCWC 1 cut(s) 460
BsiHKCI CYCGRG 1 cut(s) 332
BslFI GGGAC 1 cut(s) 108
BsmAI GTCTC 2 cut(s) 109, 430
BsmFI GGGAC 1 cut(s) 108
BsmI GAATGC 1 cut(s) 383
BsnI GGCC 1 cut(s) 290
Bso31I GGTCTC 1 cut(s) 109
BsoBI CYCGRG 1 cut(s) 332
Bsp1286I GDGCHC 3 cut(s) 174, 397, 460
Bsp143I GATC 1 cut(s) 328
Bsp19I CCATGG 1 cut(s) 207
BspACI CCGC 1 cut(s) 99
BspANI GGCC 1 cut(s) 290
BspCNI CTCAG 3 cut(s) 105, 385, 459
BspLI GGNNCC 3 cut(s) 96, 97, 265
BspPI GGATC 1 cut(s) 323
BspT107I GGYRCC 1 cut(s) 263
BspTNI GGTCTC 1 cut(s) 109
BsrDI GCAATG 1 cut(s) 291
BsrI ACTGG 1 cut(s) 163
BssECI CCNNGG 1 cut(s) 207
BssMI GATC 1 cut(s) 328
BssNI GRCGYC 2 cut(s) 25, 264
BssT1I CCWWGG 1 cut(s) 207
Bst6I CTCTTC 1 cut(s) 245
BstACI GRCGYC 2 cut(s) 25, 264
BstDEI CTNAG 3 cut(s) 113, 372, 446
BstDSI CCRYGG 1 cut(s) 207
BstH2I RGCGCY 1 cut(s) 267
BstHHI GCGC 1 cut(s) 266
BstKTI GATC 1 cut(s) 331
BstMAI GTCTC 2 cut(s) 109, 430
BstMBI GATC 1 cut(s) 328
BstMWI GCNNNNNNNGC 2 cut(s) 212, 280
BstSLI GKGCMC 1 cut(s) 174
BstV1I GCAGC 3 cut(s) 113, 215, 261
BstV2I GAAGAC 4 cut(s) 37, 227, 344, 425
BsuRI GGCC 1 cut(s) 290
BtgI CCRYGG 1 cut(s) 207
BtsIMutI CAGTG 1 cut(s) 156
CfoI GCGC 1 cut(s) 266
Cfr13I GGNCC 1 cut(s) 95
CseI GACGC 1 cut(s) 33
CviAII CATG 5 cut(s) 65, 208, 379, 415, 477
CviJI RGCY 6 cut(s) 5, 104, 206, 290, 395, 458
CviKI_1 RGCY 6 cut(s) 5, 104, 206, 290, 395, 458
DdeI CTNAG 3 cut(s) 113, 372, 446
DinI GGCGCC 1 cut(s) 265
DpnI GATC 1 cut(s) 330
DpnII GATC 1 cut(s) 328
Eam1104I CTCTTC 1 cut(s) 245
EarI CTCTTC 1 cut(s) 245
Ecl136II GAGCTC 1 cut(s) 458
Eco130I CCWWGG 1 cut(s) 207
Eco24I GRGCYC 2 cut(s) 397, 460
Eco31I GGTCTC 1 cut(s) 109
Eco47I GGWCC 1 cut(s) 95
Eco53kI GAGCTC 1 cut(s) 458
Eco88I CYCGRG 1 cut(s) 332
EcoICRI GAGCTC 1 cut(s) 458
EcoO109I RGGNCCY 1 cut(s) 95
EcoRI GAATTC 1 cut(s) 344
EcoT14I CCWWGG 1 cut(s) 207
EcoT38I GRGCYC 2 cut(s) 397, 460
EgeI GGCGCC 1 cut(s) 265
EheI GGCGCC 1 cut(s) 265
ErhI CCWWGG 1 cut(s) 207
FaeI CATG 5 cut(s) 68, 211, 382, 418, 480
FaiI YATR 7 cut(s) 66, 209, 312, 327, 380, 416, 478
FaqI GGGAC 1 cut(s) 108
FatI CATG 5 cut(s) 64, 207, 378, 414, 476
FauI CCCGC 1 cut(s) 106
Fnu4HI GCNGC 3 cut(s) 102, 204, 275
FriOI GRGCYC 2 cut(s) 397, 460
Fsp4HI GCNGC 3 cut(s) 102, 204, 275
GlaI GCGC 1 cut(s) 265
GluI GCNGC 3 cut(s) 102, 204, 275
HaeII RGCGCY 1 cut(s) 267
HaeIII GGCC 1 cut(s) 290
HgaI GACGC 1 cut(s) 33
HhaI GCGC 1 cut(s) 266
Hin1I GRCGYC 2 cut(s) 25, 264
Hin1II CATG 5 cut(s) 68, 211, 382, 418, 480
Hin6I GCGC 1 cut(s) 264
HinP1I GCGC 1 cut(s) 264
HincII GTYRAC 1 cut(s) 150
HindII GTYRAC 1 cut(s) 150
HinfI GANTC 1 cut(s) 139
Hpy166II GTNNAC 3 cut(s) 88, 150, 369
Hpy188I TCNGA 2 cut(s) 114, 375
Hpy188III TCNNGA 3 cut(s) 136, 332, 448
Hpy8I GTNNAC 3 cut(s) 88, 150, 369
Hpy99I CGWCG 1 cut(s) 131
HpyAV CCTTC 2 cut(s) 38, 214
HpyCH4IV ACGT 1 cut(s) 129
HpyCH4V TGCA 2 cut(s) 166, 283
HpyF10VI GCNNNNNNNGC 2 cut(s) 212, 280
HpyF3I CTNAG 3 cut(s) 113, 372, 446
HpySE526I ACGT 1 cut(s) 129
Hsp92I GRCGYC 2 cut(s) 25, 264
Hsp92II CATG 5 cut(s) 68, 211, 382, 418, 480
HspAI GCGC 1 cut(s) 264
KasI GGCGCC 1 cut(s) 263
KflI GGGWCCC 1 cut(s) 95
Kzo9I GATC 1 cut(s) 328
LmnI GCTCC 1 cut(s) 463
LpnPI CCDG 3 cut(s) 144, 272, 433
Lsp1109I GCAGC 3 cut(s) 113, 215, 261
LweI GCATC 1 cut(s) 292
MaeII ACGT 1 cut(s) 129
MalI GATC 1 cut(s) 330
MboI GATC 1 cut(s) 328
MboII GAAGA 8 cut(s) 37, 40, 227, 232, 246, 349, 379, 430
MhlI GDGCHC 3 cut(s) 174, 397, 460
MluCI AATT 3 cut(s) 77, 122, 344
Mly113I GGCGCC 1 cut(s) 264
MnlI CCTC 2 cut(s) 49, 62
MslI CAYNNNNRTG 2 cut(s) 159, 161
Mva1269I GAATGC 1 cut(s) 383
MwoI GCNNNNNNNGC 2 cut(s) 212, 280
NarI GGCGCC 1 cut(s) 264
NcoI CCATGG 1 cut(s) 207
NdeII GATC 1 cut(s) 328
NlaIII CATG 5 cut(s) 68, 211, 382, 418, 480
NlaIV GGNNCC 3 cut(s) 96, 97, 265
OliI CACNNNNGTG 2 cut(s) 159, 161
PcsI WCGNNNNNNNCGW 1 cut(s) 15
PctI GAATGC 1 cut(s) 383
PfeI GAWTC 1 cut(s) 139
PkrI GCNGC 3 cut(s) 103, 205, 276
PluTI GGCGCC 1 cut(s) 267
PpuMI RGGWCCY 1 cut(s) 95
Psp124BI GAGCTC 1 cut(s) 460
Psp5II RGGWCCY 1 cut(s) 95
PspN4I GGNNCC 3 cut(s) 96, 97, 265
PspPI GGNCC 1 cut(s) 95
PspPPI RGGWCCY 1 cut(s) 95
RseI CAYNNNNRTG 2 cut(s) 159, 161
SacI GAGCTC 1 cut(s) 460
SatI GCNGC 3 cut(s) 102, 204, 275
Sau3AI GATC 1 cut(s) 328
Sau96I GGNCC 1 cut(s) 95
SduI GDGCHC 3 cut(s) 174, 397, 460
SetI ASST 2 cut(s) 132, 460
SfaNI GCATC 1 cut(s) 292
SfoI GGCGCC 1 cut(s) 265
SinI GGWCC 1 cut(s) 95
SmiMI CAYNNNNRTG 2 cut(s) 159, 161
Sse9I AATT 3 cut(s) 77, 122, 344
SsiI CCGC 1 cut(s) 99
SspDI GGCGCC 1 cut(s) 263
SstI GAGCTC 1 cut(s) 460
StyI CCWWGG 1 cut(s) 207
TaiI ACGT 1 cut(s) 132
TaqI TCGA 2 cut(s) 126, 137
TasI AATT 3 cut(s) 77, 122, 344
TfiI GAWTC 1 cut(s) 139
TscAI CASTG 1 cut(s) 163
TseI GCWGC 3 cut(s) 101, 203, 274
TspDTI ATGAA 3 cut(s) 90, 232, 431
TspRI CASTG 1 cut(s) 163
VpaK11BI GGWCC 1 cut(s) 95
XapI RAATTY 3 cut(s) 77, 122, 344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.