pycom13g18810

Protein present in Fab1, YOTB, Vac1, and EEA1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0006555
Physical Location & Seq
Forward (+)
14812782 .. 14818091
5310 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3192 bp
ATGAAGGCACTCATTGCATTAAAAAGAGGTTCCCAGTTAATCAAGTATAGCCGGAAAGGAAAGCCAAAACTTCATCCATTCAGAATTTCTACTGATGAAACTACACTAATCTGGTATTCACATGGAGAAGAACGTACTCTGAAATTAGCTTCTGTCTCTCGAATTATCCCCGGACAGAGAACAGCTGTGTTTAGAAGGTATTTGCGCCCAGAAAAGGATTACTTGTCATTTTCACTTCTATATAAGAACGGTGAACGAACACTTGATCTGATTTGCAAGGACAAAGCTGAGGCAGAGATATGGTTTGCTGGCCTTAAGGCGCTAATTACTTCAGGAAAAGAGCGTAGTAGACGTAGTCAGAGTGATATTTCTGATTTGCAAGACTCTGCTGAATCTGTTCACAGCCGTCCATTTGATGCGACCTTAGAGTTCACGTCAAGCATTGCCAGTGGTAGGGTATCCGTTGATTCTCCTGAATCAGTGAATTTGACAGGCTCAGATGTGGGTTCAGAACGTGCAAATATGCAACTAAGAACAAGTGCAGGAGATGGTTTTCGAATTAGTGTTTCAAGCACTCCTAGCTGTTCTAGTGGAGGGTCTGGACATGATGATATAGAATCACTAGGAGATGTTTATGTGTGGGGAGAGATCTGGTCTGATGGAAATGGACCTGATGGGTCTGCGAATTCAATCTCTAAAAAAATCGATGTGCTGATTCCAAAGCCCTTGGAGTCGAATGTTGTTCTCGATGTTCAGCAGATAGCTTGTGGTGTGCGACATGTTGCTCTTGTAACAAGGCAAGGGGAGGTTTTCACCTGGGGAGAGGAATCGGGTGGAAGACTCGGTCATGGGATTGATAGAGATTTTAGTCGTCCTCGCCTTGTTGAGTTCCTCGCAATTAATAACATTGAATTTGTTGCATGTGGTGAGTACCACACATGTGCTGTATCTACATCAGGTGATTTGTTTACGTGGGGTGATGGTACACATAATGCTGGACTTCTTGGTCTTGGAACTGATGTTAGCCACTGGATACCTAAAAGGGTTACTGGTCCTTTAGAAGGACTTCAGGTTCTATCTGTTGCATGTGGCACATGGCATTCAGCTTTAGCAACTTCGAATGGAAAGCTATTTACATTTGGAGATGGAGCGTTCGGTGTTTTGGGCCATGGAGATCGAGAGAGTGTTCCGTATCCAAGGGAGGTACAGTCGTTGAATGGACTAAAGACTATAAAAGTAGCATGCGGAGTTTGGCATACTGCAGCTATTGTAGAAGTTATGGGCCAGTCTGGTACAAATGCTTCATCGCGTAAGTTGTTCACCTGGGGCGATGGTGACAAACATCGTTTGGGTCACGAAATCAAGGAAACTTATCTGCTTCCCACCTGTGTCTCTTCACTTATCGACTATAATTTCCACCAGCTAGCATGTGGACACACTATGACTATTGCGCTCACCACATCAGGTCATGTGTTTACCATGGGCGGAACTGCATATGGCCAGCTAGGCAATCCAAGTTCTGTTGGGAAAGCACCTTGTTTAGTACAGGATCGATTGGTTGGTGAGTTTGTCGAAGAAATATCTTGTGGGTCATATCATGTTGCTGTCCTGACATCAAGAAGTGAAGTGTTCACTTGGGGAAAAGGTGCTAATGGAAGACTGGGACACGGGGACACAGAAGACCGGAAAACTCCAACTTTGGTTGAAGCCCTGAAAGATAGGCATGTGAAAAGCATATCGTGCGGCTCAAACTTCACAGCTAGTATATGCATCCATAAGTGGGTCTCTGGAGCTGATCAATCAATTTGCTCAGGTTGTCGACAGACATTTGGTTTTACTAGAAAGAGGCATAACTGTTATAACTGTGGACTAGTCCATTGCCATGCTTGTAGTTCCAAGAAAGCACTGAGGGCAGCTTTGGCCCCGACTCCTGGAAAACCTCATCGTGTATGTGATGCTTGTTATGCGAAACTTAAAGCTGCTGAAGCTGGTAATGCTTCCAGTGTCAATAGGAGATCTAGGGTCACACGCTCAATGGATAGCAGGGATTTACTAAGTAGAGTAGAGGTGAAATCTTCAAGGATTCTTCTCTCTCCCTCTATAGAACCCGTTATATACCTTGAGGTCAAGTCCATGAAGCCTGGTGGTGTTAGATCCGAATCCCCTTCTATAGTCAGGTCTTCCCAAGTTCCATCCCTTTTACAACTTAAAGATATTGCATTTCCAAGTTCGTTGAGTGCACTGCAAAACGCTTTGAAGCCTGTCATGACAACGCCATCTCAGCCCAATTCGAGATCTAATTCACCATATTCAAGGAGACCTAGTCCTCCCCGGTCTACAACTCCTATATTCTCTAGGAGTGTTATTGACAGTCTTAAGAGGACAAATGACACTCTGACACAAGAAGTATCAAAGTTGCAAAACCAGGTTAAAAGTCTGAAGCATAAGTGTGATGCTCAAGAGGTAGAGATAAAGAAACTACGTAAACATGCGAAAGAAGCTGGCTCATTAGCCGATGACCGATACTTGAAGTGCAGAGCTGCCAAAGAACTCGTCAAGTCCATCACGGAACAGATGAGGGAATGGGAAGACAAGATACCTCCTGAGATTTCTGACAGTGATACTTTCAAAGCATTGCGTACGCAAGCTGAGGATTTTATGAACATGAATATTGGAAGGCCAAGCTCAGAGCTTGACCAGCACTATGCAACCGATAGAACTTCCTTGGTTGTTGAGTCTTCTAGAATGGAAGACAACAAAGCAGAAGACTCTGCAGAGGCTGAACCGCAAAACAGCTCAGAAAACCACTCGAGGTCACCTGAATCATCAACGTTGCGGACGGTAGACAACAGATCAGAAGACTTTGCGGATGCTGGACCTCAAAACCGCTCAAGGTCACCAGAATCATCAATATCACGGACAGGACAAAAGGAAGTCATTGAACAATTTGAACCGGGCGTTTATGTAACTCTCCTTCAACTAAAAAATGGTATTCGGGTTTTTAAGCGTGTTAAATTCAGCAAACGGAAGTTTAATTCGGAACAGGCAGAAGCATGGTGGAAAAACAACAAAGATAGGCTGCTTAGGAGATATAGTCAGCCGAATGCAAGTCCTGCTCCGAGTGCACCACCACCCAGCAGTCCTATTCCGCCCGAGGAAAACAGCGAGGCAGCCGCAGCTTCCCCGACTTAG

Protein Analysis

1064

Amino Acids

116.43

Weight (kDa)

8.89

Isoelectric Point (pI)

53.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH_12 PF16457 30 - 109 1.7e-06 Pleckstrin homology domain
WD40_RLD PF25390 211 - 587 2.1e-77 RCC1-like domain
RCC1 PF00415 267 - 316 1.4e-11 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 306 - 330 1e-07 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 319 - 370 1.1e-09 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 374 - 423 1.5e-14 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 439 - 485 2.8e-09 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 488 - 537 7.8e-07 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 542 - 588 4e-15 Regulator of chromosome condensation (RCC1) repeat
FYVE PF01363 593 - 659 3e-15 FYVE zinc finger
BRX_N PF13713 844 - 875 8.5e-08 Transcription factor BRX N-terminal domain
BRX PF08381 977 - 1031 7.7e-23 Transcription factor regulating root and shoot growth via Pin3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1860
AasI GACNNNNNNGTC 1 cut(s) 1005
AccBSI CCGCTC 1 cut(s) 2889
AccI GTMKAC 4 cut(s) 349, 1819, 2336, 2844
AccII CGCG 1 cut(s) 1309
AciI CCGC 9 cut(s) 1245, 1485, 1743, 2786, 2836, 2867, 2887, 3149, 3174
AclI AACGTT 1 cut(s) 2831
AclWI GGATC 2 cut(s) 1557, 2148
AcoI YGGCCR 1 cut(s) 1498
AcsI RAATTY 5 cut(s) 84, 484, 685, 911, 3014
AcuI CTGAAG 4 cut(s) 315, 1052, 2004, 2459
AfaI GTAC 7 cut(s) 136, 932, 985, 1206, 1294, 1545, 2641
AfiI CCNNNNNNNGG 5 cut(s) 214, 453, 1061, 1780, 1931
AflII CTTAAG 2 cut(s) 314, 2375
AflIII ACRYGT 2 cut(s) 778, 938
AhlI ACTAGT 1 cut(s) 1870
AjiI CACGTC 1 cut(s) 435
AjnI CCWGG 5 cut(s) 815, 1322, 1930, 2140, 2424
AleI CACNNNNGTG 1 cut(s) 939
AloI GAACNNNNNNTCC 2 cut(s) 1056, 1088
Alw21I GWGCWC 2 cut(s) 2242, 3127
Alw26I GTCTC 4 cut(s) 160, 1396, 1789, 2311
Alw44I GTGCAC 2 cut(s) 2238, 3123
AlwI GGATC 2 cut(s) 1557, 2148
AlwNI CAGNNNCTG 1 cut(s) 2780
Ama87I CYCGRG 2 cut(s) 2809, 3152
AoxI GGCC 6 cut(s) 310, 1165, 1282, 1498, 1920, 2678
ApaLI GTGCAC 2 cut(s) 2238, 3123
ApeKI GCWGC 7 cut(s) 1262, 1913, 1979, 2540, 3079, 3170, 3176
ApoI RAATTY 5 cut(s) 84, 484, 685, 911, 3014
ArsI GACNNNNNNTTYG 2 cut(s) 2538, 2570
AseI ATTAAT 1 cut(s) 900
Asp700I GAANNNNTTC 2 cut(s) 396, 1065
AspLEI GCGC 3 cut(s) 207, 322, 1453
AspS9I GGNCC 6 cut(s) 668, 1052, 1165, 1282, 1921, 2876
AsuC2I CCSGG 3 cut(s) 171, 2332, 2955
AsuII TTCGAA 2 cut(s) 556, 1118
AsuNHI GCTAGC 1 cut(s) 1423
AvaI CYCGRG 2 cut(s) 2809, 3152
AvaII GGWCC 3 cut(s) 668, 1052, 2876
BaeGI GKGCMC 2 cut(s) 2242, 3127
BaeI ACNNNNGTAYC 2 cut(s) 1196, 1229
BalI TGGCCA 1 cut(s) 1500
BarI GAAGNNNNNNTAC 6 cut(s) 1285, 1317, 2580, 2612, 2958, 2990
BbsI GAAGAC 9 cut(s) 844, 1663, 1686, 2172, 2595, 2730, 2757, 2772, 2865
Bbv12I GWGCWC 2 cut(s) 2242, 3127
BbvCI CCTCAGC 2 cut(s) 288, 2649
BbvI GCAGC 7 cut(s) 1274, 1925, 1966, 2527, 3066, 3182, 3188
BccI CCATC 9 cut(s) 542, 653, 668, 974, 1139, 1325, 2200, 2284, 2570
BceAI ACGGC 1 cut(s) 390
BciT130I CCWGG 5 cut(s) 817, 1324, 1932, 2142, 2426
BciVI GTATCC 3 cut(s) 469, 1026, 1203
BclI TGATCA 1 cut(s) 1795
BcnI CCSGG 3 cut(s) 171, 2332, 2955
BcoDI GTCTC 4 cut(s) 160, 1396, 1789, 2311
BcuI ACTAGT 1 cut(s) 1870
BfmI CTRYAG 4 cut(s) 1260, 2100, 2169, 2772
BfoI RGCGCY 1 cut(s) 323
BfrI CTTAAG 2 cut(s) 314, 2375
BfuI GTATCC 3 cut(s) 469, 1026, 1203
BglI GCCNNNNNGGC 1 cut(s) 1506
BglII AGATCT 3 cut(s) 648, 2015, 2294
BisI GCNGC 9 cut(s) 1263, 1744, 1914, 1980, 2541, 3080, 3171, 3174, 3177
BlsI GCNGC 9 cut(s) 1264, 1745, 1915, 1981, 2542, 3081, 3172, 3175, 3178
Bme1390I CCNGG 8 cut(s) 171, 817, 1324, 1932, 2142, 2332, 2426, 2955
Bme18I GGWCC 3 cut(s) 668, 1052, 2876
BmeT110I CYCGRG 2 cut(s) 2809, 3152
BmgBI CACGTC 1 cut(s) 435
BmgT120I GGNCC 6 cut(s) 668, 1052, 1165, 1282, 1921, 2876
BmiI GGNNCC 2 cut(s) 31, 1923
BmrFI CCNGG 8 cut(s) 171, 817, 1324, 1932, 2142, 2332, 2426, 2955
BmrI ACTGGG 2 cut(s) 28, 1670
BmsI GCATC 5 cut(s) 406, 1779, 1945, 2443, 2860
BmtI GCTAGC 1 cut(s) 1427
BmuI ACTGGG 2 cut(s) 28, 1670
BpiI GAAGAC 9 cut(s) 844, 1663, 1686, 2172, 2595, 2730, 2757, 2772, 2865
BpmI CTGGAG 1 cut(s) 1809
Bpu10I CCTNAGC 4 cut(s) 288, 1810, 2649, 3083
Bpu14I TTCGAA 2 cut(s) 556, 1118
BpuEI CTTGAG 3 cut(s) 2141, 2442, 2875
BpuMI CCSGG 3 cut(s) 171, 2332, 2955
Bsa29I ATCGAT 2 cut(s) 705, 1552
BsaAI YACGTR 2 cut(s) 972, 2483
BsaBI GATNNNNATC 1 cut(s) 2158
BsaI GGTCTC 2 cut(s) 1789, 2311
BsaWI WCCGGW 1 cut(s) 1683
BsaXI ACNNNNNCTCC 6 cut(s) 1193, 1223, 2308, 2338, 3079, 3109
Bsc4I CCNNNNNNNGG 5 cut(s) 214, 453, 1061, 1780, 1931
Bse1I ACTGG 7 cut(s) 34, 447, 1034, 1054, 1285, 1665, 2001
Bse3DI GCAATG 4 cut(s) 12, 441, 1876, 2633
Bse8I GATNNNNATC 1 cut(s) 2158
BseBI CCWGG 5 cut(s) 817, 1324, 1932, 2142, 2426
BseCI ATCGAT 2 cut(s) 705, 1552
BseGI GGATG 4 cut(s) 73, 1770, 2192, 2875
BseJI GATNNNNATC 1 cut(s) 2158
BseLI CCNNNNNNNGG 5 cut(s) 214, 453, 1061, 1780, 1931
BseMI GCAATG 4 cut(s) 12, 441, 1876, 2633
BseMII CTCAG 9 cut(s) 279, 510, 1824, 1898, 2294, 2595, 2640, 2700, 2811
BseNI ACTGG 7 cut(s) 34, 447, 1034, 1054, 1285, 1665, 2001
BseSI GKGCMC 2 cut(s) 2242, 3127
BseXI GCAGC 7 cut(s) 1274, 1925, 1966, 2527, 3066, 3182, 3188
BseYI CCCAGC 1 cut(s) 3134
BsgI GTGCAG 2 cut(s) 561, 2554
Bsh1236I CGCG 1 cut(s) 1309
BshFI GGCC 6 cut(s) 312, 1167, 1284, 1500, 1922, 2680
BshVI ATCGAT 2 cut(s) 705, 1552
BsiHKAI GWGCWC 2 cut(s) 2242, 3127
BsiHKCI CYCGRG 2 cut(s) 2809, 3152
BsiSI CCGG 5 cut(s) 52, 171, 1684, 2332, 2954
BsiWI CGTACG 1 cut(s) 2639
BslFI GGGAC 2 cut(s) 1677, 1685
BslI CCNNNNNNNGG 5 cut(s) 214, 453, 1061, 1780, 1931
BsmAI GTCTC 4 cut(s) 160, 1396, 1789, 2311
BsmFI GGGAC 2 cut(s) 1677, 1685
BsmI GAATGC 2 cut(s) 1099, 3109
BsnI GGCC 6 cut(s) 312, 1167, 1284, 1500, 1922, 2680
Bso31I GGTCTC 2 cut(s) 1789, 2311
BsoBI CYCGRG 2 cut(s) 2809, 3152
Bsp119I TTCGAA 2 cut(s) 556, 1118
Bsp1286I GDGCHC 2 cut(s) 2242, 3127
Bsp143I GATC 9 cut(s) 265, 648, 1174, 1549, 1795, 2015, 2153, 2294, 2852
Bsp19I CCATGG 2 cut(s) 1168, 1479
BspACI CCGC 9 cut(s) 1245, 1485, 1743, 2786, 2836, 2867, 2887, 3149, 3174
BspANI GGCC 6 cut(s) 312, 1167, 1284, 1500, 1922, 2680
BspCNI CTCAG 9 cut(s) 280, 509, 1823, 1899, 2293, 2596, 2641, 2699, 2810
BspDI ATCGAT 2 cut(s) 705, 1552
BspFNI CGCG 1 cut(s) 1309
BspHI TCATGA 1 cut(s) 2265
BspLI GGNNCC 2 cut(s) 31, 1923
BspMAI CTGCAG 2 cut(s) 1264, 2776
BspOI GCTAGC 1 cut(s) 1427
BspPI GGATC 2 cut(s) 1557, 2148
BspT104I TTCGAA 2 cut(s) 556, 1118
BspTI CTTAAG 2 cut(s) 314, 2375
BspTNI GGTCTC 2 cut(s) 1789, 2311
BsrBI CCGCTC 1 cut(s) 2889
BsrDI GCAATG 4 cut(s) 12, 441, 1876, 2633
BsrI ACTGG 7 cut(s) 34, 447, 1034, 1054, 1285, 1665, 2001
BssMI GATC 9 cut(s) 265, 648, 1174, 1549, 1795, 2015, 2153, 2294, 2852
BssT1I CCWWGG 5 cut(s) 726, 1168, 1196, 1479, 2724
Bst2UI CCWGG 5 cut(s) 817, 1324, 1932, 2142, 2426
Bst4CI ACNGT 7 cut(s) 251, 1209, 1856, 1865, 2372, 2618, 2842
Bst6I CTCTTC 1 cut(s) 1399
BstAFI CTTAAG 2 cut(s) 314, 2375
BstAPI GCANNNNNTGC 3 cut(s) 14, 1740, 3113
BstBAI YACGTR 2 cut(s) 972, 2483
BstBI TTCGAA 2 cut(s) 556, 1118
BstC8I GCNNGC 6 cut(s) 310, 1243, 1425, 1502, 2503, 2646
BstDSI CCRYGG 2 cut(s) 1168, 1479
BstEII GGTNACC 2 cut(s) 2814, 2895
BstENI CCTNNNNNAGG 1 cut(s) 1059
BstF5I GGATG 4 cut(s) 73, 1770, 2192, 2875
BstFNI CGCG 1 cut(s) 1309
BstH2I RGCGCY 1 cut(s) 323
BstHHI GCGC 3 cut(s) 207, 322, 1453
BstKTI GATC 9 cut(s) 268, 651, 1177, 1552, 1798, 2018, 2156, 2297, 2855
BstMAI GTCTC 4 cut(s) 160, 1396, 1789, 2311
BstMBI GATC 9 cut(s) 265, 648, 1174, 1549, 1795, 2015, 2153, 2294, 2852
BstNI CCWGG 5 cut(s) 817, 1324, 1932, 2142, 2426
BstNSI RCATGY 8 cut(s) 782, 924, 942, 1089, 1245, 1431, 1727, 2492
BstPI GGTNACC 2 cut(s) 2814, 2895
BstSCI CCNGG 8 cut(s) 169, 815, 1322, 1930, 2140, 2330, 2424, 2953
BstSFI CTRYAG 4 cut(s) 1260, 2100, 2169, 2772
BstSLI GKGCMC 2 cut(s) 2242, 3127
BstSNI TACGTA 1 cut(s) 2483
BstUI CGCG 1 cut(s) 1309
BstV1I GCAGC 7 cut(s) 1274, 1925, 1966, 2527, 3066, 3182, 3188
BstV2I GAAGAC 9 cut(s) 844, 1663, 1686, 2172, 2595, 2730, 2757, 2772, 2865
BstX2I RGATCY 4 cut(s) 648, 2015, 2153, 2294
BstYI RGATCY 4 cut(s) 648, 2015, 2153, 2294
Bsu15I ATCGAT 2 cut(s) 705, 1552
BsuI GTATCC 3 cut(s) 469, 1026, 1203
BsuRI GGCC 6 cut(s) 312, 1167, 1284, 1500, 1922, 2680
BsuTUI ATCGAT 2 cut(s) 705, 1552
BtgI CCRYGG 2 cut(s) 1168, 1479
BtgZI GCGATG 2 cut(s) 1290, 1344
BtrI CACGTC 1 cut(s) 435
BtsCI GGATG 4 cut(s) 73, 1770, 2192, 2875
BtsI GCAGTG 1 cut(s) 2240
BtsIMutI CAGTG 7 cut(s) 454, 486, 1027, 1904, 2008, 2240, 2623
Cac8I GCNNGC 6 cut(s) 310, 1243, 1425, 1502, 2503, 2646
CaiI CAGNNNCTG 1 cut(s) 2780
CciI TCATGA 1 cut(s) 2265
CfoI GCGC 3 cut(s) 207, 322, 1453
Cfr13I GGNCC 6 cut(s) 668, 1052, 1165, 1282, 1921, 2876
ClaI ATCGAT 2 cut(s) 705, 1552
CsiI ACCWGGT 1 cut(s) 2424
Csp6I GTAC 7 cut(s) 135, 931, 984, 1205, 1293, 1544, 2640
CviQI GTAC 7 cut(s) 135, 931, 984, 1205, 1293, 1544, 2640
DpnI GATC 9 cut(s) 267, 650, 1176, 1551, 1797, 2017, 2155, 2296, 2854
DpnII GATC 9 cut(s) 265, 648, 1174, 1549, 1795, 2015, 2153, 2294, 2852
DrdI GACNNNNNNGTC 1 cut(s) 1005
DseDI GACNNNNNNGTC 1 cut(s) 1005
EaeI YGGCCR 1 cut(s) 1498
Eam1104I CTCTTC 1 cut(s) 1399
EarI CTCTTC 1 cut(s) 1399
EciI GGCGGA 2 cut(s) 1500, 3138
Eco105I TACGTA 1 cut(s) 2483
Eco130I CCWWGG 5 cut(s) 726, 1168, 1196, 1479, 2724
Eco31I GGTCTC 2 cut(s) 1789, 2311
Eco47I GGWCC 3 cut(s) 668, 1052, 2876
Eco57I CTGAAG 4 cut(s) 315, 1052, 2004, 2459
Eco88I CYCGRG 2 cut(s) 2809, 3152
Eco91I GGTNACC 2 cut(s) 2814, 2895
EcoNI CCTNNNNNAGG 1 cut(s) 1059
EcoO65I GGTNACC 2 cut(s) 2814, 2895
EcoRI GAATTC 1 cut(s) 685
EcoRII CCWGG 5 cut(s) 815, 1322, 1930, 2140, 2424
EcoT14I CCWWGG 5 cut(s) 726, 1168, 1196, 1479, 2724
EcoT22I ATGCAT 1 cut(s) 1772
ErhI CCWWGG 5 cut(s) 726, 1168, 1196, 1479, 2724
FalI AAGNNNNNCTT 2 cut(s) 206, 238
FaqI GGGAC 2 cut(s) 1677, 1685
FauNDI CATATG 1 cut(s) 1495
FbaI TGATCA 1 cut(s) 1795
FblI GTMKAC 4 cut(s) 349, 1819, 2336, 2844
Fnu4HI GCNGC 9 cut(s) 1263, 1744, 1914, 1980, 2541, 3080, 3171, 3174, 3177
FokI GGATG 4 cut(s) 60, 1757, 2179, 2882
Fsp4HI GCNGC 9 cut(s) 1263, 1744, 1914, 1980, 2541, 3080, 3171, 3174, 3177
GlaI GCGC 3 cut(s) 206, 321, 1452
GluI GCNGC 9 cut(s) 1263, 1744, 1914, 1980, 2541, 3080, 3171, 3174, 3177
GsaI CCCAGC 1 cut(s) 3138
GsuI CTGGAG 1 cut(s) 1809
HaeII RGCGCY 1 cut(s) 323
HaeIII GGCC 6 cut(s) 312, 1167, 1284, 1500, 1922, 2680
HapII CCGG 5 cut(s) 52, 171, 1684, 2332, 2954
HhaI GCGC 3 cut(s) 207, 322, 1453
Hin6I GCGC 3 cut(s) 205, 320, 1451
HinP1I GCGC 3 cut(s) 205, 320, 1451
HincII GTYRAC 1 cut(s) 1820
HindII GTYRAC 1 cut(s) 1820
HpaII CCGG 5 cut(s) 52, 171, 1684, 2332, 2954
HpyAV CCTTC 5 cut(s) 189, 1055, 2175, 2670, 2984
HpyCH4III ACNGT 7 cut(s) 251, 1209, 1856, 1865, 2372, 2618, 2842
HpyCH4IV ACGT 7 cut(s) 133, 352, 434, 514, 971, 2482, 2831
HpySE526I ACGT 7 cut(s) 133, 352, 434, 514, 971, 2482, 2831
HspAI GCGC 3 cut(s) 205, 320, 1451
Ksp22I TGATCA 1 cut(s) 1795
Kzo9I GATC 9 cut(s) 265, 648, 1174, 1549, 1795, 2015, 2153, 2294, 2852
LmnI GCTCC 3 cut(s) 1148, 1790, 3121
Lsp1109I GCAGC 7 cut(s) 1274, 1925, 1966, 2527, 3066, 3182, 3188
LweI GCATC 5 cut(s) 406, 1779, 1945, 2443, 2860
MabI ACCWGGT 1 cut(s) 2424
MaeII ACGT 7 cut(s) 133, 352, 434, 514, 971, 2482, 2831
MaeIII GTNAC 8 cut(s) 790, 1045, 1334, 1352, 2023, 2814, 2895, 2965
MalI GATC 9 cut(s) 267, 650, 1176, 1551, 1797, 2017, 2155, 2296, 2854
MbiI CCGCTC 1 cut(s) 2889
MboI GATC 9 cut(s) 265, 648, 1174, 1549, 1795, 2015, 2153, 2294, 2852
MflI RGATCY 4 cut(s) 648, 2015, 2153, 2294
MhlI GDGCHC 2 cut(s) 2242, 3127
MlsI TGGCCA 1 cut(s) 1500
MluNI TGGCCA 1 cut(s) 1500
MlyI GAGTC 6 cut(s) 377, 740, 834, 1921, 2744, 2762
MmeI TCCRAC 1 cut(s) 1718
Mox20I TGGCCA 1 cut(s) 1500
Mph1103I ATGCAT 1 cut(s) 1772
MroXI GAANNNNTTC 2 cut(s) 396, 1065
MscI TGGCCA 1 cut(s) 1500
MslI CAYNNNNRTG 3 cut(s) 939, 1881, 2448
Msp20I TGGCCA 1 cut(s) 1500
MspA1I CMGCKG 1 cut(s) 185
MspCI CTTAAG 2 cut(s) 314, 2375
MspI CCGG 5 cut(s) 52, 171, 1684, 2332, 2954
MspR9I CCNGG 8 cut(s) 171, 817, 1324, 1932, 2142, 2332, 2426, 2955
Mva1269I GAATGC 2 cut(s) 1099, 3109
MvaI CCWGG 5 cut(s) 817, 1324, 1932, 2142, 2426
MvnI CGCG 1 cut(s) 1309
NciI CCSGG 3 cut(s) 171, 2332, 2955
NcoI CCATGG 2 cut(s) 1168, 1479
NdeI CATATG 1 cut(s) 1495
NdeII GATC 9 cut(s) 265, 648, 1174, 1549, 1795, 2015, 2153, 2294, 2852
NheI GCTAGC 1 cut(s) 1423
NlaIV GGNNCC 2 cut(s) 31, 1923
NmuCI GTSAC 5 cut(s) 1334, 1352, 2023, 2814, 2895
NsiI ATGCAT 1 cut(s) 1772
NspI RCATGY 8 cut(s) 782, 924, 942, 1089, 1245, 1431, 1727, 2492
NspV TTCGAA 2 cut(s) 556, 1118
OliI CACNNNNGTG 1 cut(s) 939
PaeI GCATGC 1 cut(s) 1245
PaeR7I CTCGAG 1 cut(s) 2809
PagI TCATGA 1 cut(s) 2265
PciI ACATGT 2 cut(s) 778, 938
PctI GAATGC 2 cut(s) 1099, 3109
PdmI GAANNNNTTC 2 cut(s) 396, 1065
Pfl23II CGTACG 1 cut(s) 2639
PflFI GACNNNGTC 3 cut(s) 354, 843, 2322
PfoI TCCNGGA 1 cut(s) 1930
PkrI GCNGC 9 cut(s) 1264, 1745, 1915, 1981, 2542, 3081, 3172, 3175, 3178
PleI GAGTC 6 cut(s) 377, 739, 834, 1921, 2743, 2762
PpsI GAGTC 6 cut(s) 377, 739, 834, 1921, 2743, 2762
Ppu21I YACGTR 2 cut(s) 972, 2483
PscI ACATGT 2 cut(s) 778, 938
PshBI ATTAAT 1 cut(s) 900
PsiI TTATAA 1 cut(s) 1860
Psp1406I AACGTT 1 cut(s) 2831
Psp6I CCWGG 5 cut(s) 815, 1322, 1930, 2140, 2424
PspEI GGTNACC 2 cut(s) 2814, 2895
PspFI CCCAGC 1 cut(s) 3134
PspGI CCWGG 5 cut(s) 815, 1322, 1930, 2140, 2424
PspLI CGTACG 1 cut(s) 2639
PspN4I GGNNCC 2 cut(s) 31, 1923
PspPI GGNCC 6 cut(s) 668, 1052, 1165, 1282, 1921, 2876
PspXI VCTCGAGB 1 cut(s) 2809
PstI CTGCAG 2 cut(s) 1264, 2776
PstNI CAGNNNCTG 1 cut(s) 2780
PsuI RGATCY 4 cut(s) 648, 2015, 2153, 2294
PsyI GACNNNGTC 3 cut(s) 354, 843, 2322
PvuII CAGCTG 1 cut(s) 185
RsaI GTAC 7 cut(s) 136, 932, 985, 1206, 1294, 1545, 2641
RsaNI GTAC 7 cut(s) 135, 931, 984, 1205, 1293, 1544, 2640
RseI CAYNNNNRTG 3 cut(s) 939, 1881, 2448
SalI GTCGAC 1 cut(s) 1818
SatI GCNGC 9 cut(s) 1263, 1744, 1914, 1980, 2541, 3080, 3171, 3174, 3177
Sau3AI GATC 9 cut(s) 265, 648, 1174, 1549, 1795, 2015, 2153, 2294, 2852
Sau96I GGNCC 6 cut(s) 668, 1052, 1165, 1282, 1921, 2876
SchI GAGTC 6 cut(s) 377, 740, 834, 1921, 2744, 2762
ScrFI CCNGG 8 cut(s) 171, 817, 1324, 1932, 2142, 2332, 2426, 2955
SduI GDGCHC 2 cut(s) 2242, 3127
SexAI ACCWGGT 1 cut(s) 2424
SfaNI GCATC 5 cut(s) 406, 1779, 1945, 2443, 2860
SfcI CTRYAG 4 cut(s) 1260, 2100, 2169, 2772
Sfr274I CTCGAG 1 cut(s) 2809
SfuI TTCGAA 2 cut(s) 556, 1118
SinI GGWCC 3 cut(s) 668, 1052, 2876
SlaI CTCGAG 1 cut(s) 2809
SmiMI CAYNNNNRTG 3 cut(s) 939, 1881, 2448
SmlI CTYRAG 6 cut(s) 314, 2120, 2375, 2457, 2809, 2890
SmoI CTYRAG 6 cut(s) 314, 2120, 2375, 2457, 2809, 2890
SnaBI TACGTA 1 cut(s) 2483
SpeI ACTAGT 1 cut(s) 1870
SphI GCATGC 1 cut(s) 1245
SsiI CCGC 9 cut(s) 1245, 1485, 1743, 2786, 2836, 2867, 2887, 3149, 3174
SspI AATATT 1 cut(s) 2671
StyD4I CCNGG 8 cut(s) 169, 815, 1322, 1930, 2140, 2330, 2424, 2953
StyI CCWWGG 5 cut(s) 726, 1168, 1196, 1479, 2724
TaaI ACNGT 7 cut(s) 251, 1209, 1856, 1865, 2372, 2618, 2842
TaiI ACGT 7 cut(s) 136, 355, 437, 517, 974, 2485, 2834
TaqII GACCGA 2 cut(s) 833, 2535
TatI WGTACW 1 cut(s) 1543
TauI GCSGC 2 cut(s) 1746, 3176
TscAI CASTG 7 cut(s) 454, 486, 1034, 1911, 2008, 2247, 2623
TseFI GTSAC 5 cut(s) 1334, 1352, 2023, 2814, 2895
TseI GCWGC 7 cut(s) 1262, 1913, 1979, 2540, 3079, 3170, 3176
Tsp45I GTSAC 5 cut(s) 1334, 1352, 2023, 2814, 2895
TspDTI ATGAA 7 cut(s) 17, 62, 111, 1293, 2150, 2675, 2681
TspGWI ACGGA 5 cut(s) 451, 1179, 2582, 2932, 3040
TspRI CASTG 7 cut(s) 454, 486, 1034, 1911, 2008, 2247, 2623
Tth111I GACNNNGTC 3 cut(s) 354, 843, 2322
Vha464I CTTAAG 2 cut(s) 314, 2375
VneI GTGCAC 2 cut(s) 2238, 3123
VpaK11BI GGWCC 3 cut(s) 668, 1052, 2876
VspI ATTAAT 1 cut(s) 900
XagI CCTNNNNNAGG 1 cut(s) 1059
XapI RAATTY 5 cut(s) 84, 484, 685, 911, 3014
XbaI TCTAGA 1 cut(s) 2741
XceI RCATGY 8 cut(s) 782, 924, 942, 1089, 1245, 1431, 1727, 2492
XcmI CCANNNNNNNNNTGG 1 cut(s) 1427
XhoI CTCGAG 1 cut(s) 2809
XmiI GTMKAC 4 cut(s) 349, 1819, 2336, 2844
XmnI GAANNNNTTC 2 cut(s) 396, 1065
Zsp2I ATGCAT 1 cut(s) 1772
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.