pycom13g20320

Domain of unknown function (DUF4487)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0006689
Physical Location & Seq
Reverse (-)
16357724 .. 16363429
5706 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3093 bp
ATGGAAGGAGGGAGCTCAAGCAGCGAATTGCAGAGCATAGAAGACGCCATTAGATCCTCGGATGTAAGCAATTCCAGTTTTCCATTTCCCAATTTCACTGAATTCAATTTTAACCTTAGGGGTTTTCATAGTAGCAGAAATCTGAATTGGCATCCCTTGCGAAATGTCTCACACTCGACATTTTGGGAGGAGTATACTTGCTTGGATGTAAGCCAGTGCATGTTAAACAAGGCTATATTACACGTGGCTGTAAAACACCTAGAATCTGACATATCCAACGCTCTAGCACATTTCCTTGCCCTTGGGACCAAGGCTACCATATGGTGTGGTAAACACCTAAAAATGACTCTTATGTCGAGTGTGGAGTCTCAAGAGGAAGAGCACGACAATCTGTTCTTTGAGCTGCTTCTGGATCTTCTGAGCTTCTCTGGTGTTAGTTTCTCAGCTTTGGCAAGATTTCCTGTTTCTACTGACAAGCTGTCAATGGATATTGTTGAGAAATTCTTAGTGGAACAGTTAAACTTGATTAAAGATTCAATAACAGAGATTAAGAGAATTGAGCCATTTGAATCTGTAGGAAAGGTCACGTTGGAGGTCATTGATGCTGTAATAAGACTATGTGGAACATATGCTCGAGTTGTAAATTGGGAGTCTTGGGAAGAAAAGCTTGAGAGGGACAAAAGTGGAACACACTTTGAAGGGGTCAGTAACATGAATCACGTTATTACTGTTATAAAGTATACAATAGAAAAATTGTGTGAGATAGGCGTTGCTGCTGCTAATAATGGAGGCAGTCTGGTAAAGGTTCTCAATTTTACATGGAAAGGTGTAGTCAGTCTACTTCAGCTTGGTGAGGGGGTATTCGCAACAAAGGTGAATGTAGCTGATATAGTTTCAAATCTAATCTCATTGGTCAATGAATCTTTAAAATGTGCAGCTGAGGCTTGGTCATCATCTTTGAATGACTCCATTTCAGTGACAGAAGCCAGGAAGACATTTCTTCCAATAAAGTTTTATCTGATTAATGCAATAAAAATATCTACCCTGTATCCGTGTCAAGCACATCTGGTACAAAGGGAGATAACAAATTGCATCCTGATGATCTCAAACTTCAAAATTTCATTGAGCAATGAAAAACTGTTAAAAACCGCCGCTGAAGTTTTCGTAGAACTCTTGGAGAAAGCATCGTTGGATTTACTATTTTCGTTACTGAATTCATCTCAAGTGAAGCAGGAGTTCAAGGTTGAGATTCTAGATTCTCTGTTCGGTAAAGGAAGCTACACAGATACTAGCTCCGGAGATATGAGTAAATTTAATGAGATAAGTTCGTTGGATGAAATCATTTCTTTATTTGGGGAAGCAATGCCTGGAGAAAGAGCCTTGTTGCTTGGTCGTGTTTCGCTATTTCTTAGTTTGTTGAAATTTTCTGTTGATCTTGAAGAAGATGTAAAACTTGGGATTACTAGAAAGCTTGGTTGGTTCTTGGATATATTAATTGATGAAGAGGTATATTCTTCCATTTTGCTTTTGCAAGTTCCCGTGTTATATGGTTCTGGAGAAACTTTGATAGTTGCCTGGCAGCCTATGTTTTCTTCGCTTTTGAATGCATTGGAAATCTTCATGCTTGTGGTCTCTTCGACTCCTGTTTGGAGGGAGTTGGAGGCTTTCCTTCTTGAGAATATATTTCATCCTCACTTCCTTTGCTGGGAGGTTGTGATGGAACTTTGGTGCTTTATGCTGCGGTATGCTGAGCCAGGGACGGTGAGTGGCATTATTGGTAAACTCTGCTCATTACTGAAGTTAGTGGCATCTTCTGAATCAGTTCTTGTACCTGGTTCTGCTCTGCGAAAATTGGCAAGATCAATCAGCATGCTTCTTACTTTTGGCTCACCGTCTATCGTAGACCAGGTTTACATGTCCATTGTTAGTGATGAGGGAACTCAGTCGTCACCAGTTATACGTCTAGCCTTATTCATGGAAGGATTTCCACTGAATTTACTCTCTGACAAGTTGAAAAGTATTGCAACTCAAAGAATCCTTACTGATTACTATGTCTTCATTGAAAATTTTGATGACAAATCTATGAGATCTTCTGGTTCTGGTGTTTTTGGTGCCCCTGTGTTTGCTTTGTCTGCTTCATTGGAGTCTCTCTTTTCCTTCTCCTGTAGCCGGATCAGTATATCCGATATCGATGTGAAGACTCTGAAGCTTTTAGTTTCCATCATTCATAACTACATGGTGTCTTCAGACAAACTAATGAAGGACCGCTACCGTAAGCTTTTGAGTGAAACATTGGTAATCATCTCAAAAATGAAGCACCTATATGCATCTGACGAAATGGAGAAAGTAATCTTTGAGCTCGAAAACCTGTTTATTTCAGGATCTGCTGCAGCTTCTGATACACAATTGTATGAATGCAAACCAAATCTGGCTCTTTTCATGGCAGGCCTTGCTCATATGGAAATCAATGAAACTAATGAAAGTGCCAAGATCTCTACTCTCTGGGAGTTGTATCACATGTTGTTGAGTGAACGGCATTGGGCATTTATTCACTTGGCTATTACAGCATTTGGATACTTTTCTGCTCGGACTGGTTGCAATGAGCTTTGGAGATTTGTGCCGGAGACTGCAGCGCTTTCATATGATTTAGAATCTGGAAATGAGGCAACTGTGGAGAGATTCATGTCTGAGTTTAAGATATATCTCGAGAAGGAAACAGCGCTTCTTACAATGACACCTTGTTCTGACCAGCTTGGTCTGCTTGCAAGGGAGGGTCTAACTCTGAAAGAAATGTTTCAGAAGATTTCAGATACCATTGTGGATACTTCAGAACGTGACAACATGGAAATCGACTGTGAAAAGCAAACGGAGATCAATGGCGGGAAGCAAACCAATAAGAAAAGGAAACTTCCGGATGGAATTAGGAAGGGAATGGAATTGCTAGAGAGTGGTATGAAGGTTATTGTTGATGGTCTGTCCCAGTGGCAGCAAACACAATTTTGCTCCAATGAACTTCACGACAAGCTCTTGACGAGCTTTTCTCGCCTTGAAGATGAAATTTCTCAACTAGTTGGCCTGGCTGGTAGTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4487

Protein Analysis

1031

Amino Acids

115.4

Weight (kDa)

5.07

Isoelectric Point (pI)

34.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4487 PF14868 268 - 869 2e-152 Domain of unknown function (DUF4487)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 734
AasI GACNNNNNNGTC 1 cut(s) 352
AccB1I GGYRCC 1 cut(s) 2111
AccI GTMKAC 4 cut(s) 194, 740, 838, 1902
AccIII TCCGGA 2 cut(s) 1295, 2911
AciI CCGC 5 cut(s) 1149, 1152, 1741, 2266, 2880
AclWI GGATC 4 cut(s) 48, 420, 2180, 2389
AcsI RAATTY 9 cut(s) 101, 500, 1116, 1213, 1310, 1421, 1993, 2065, 3057
AcuI CTGAAG 6 cut(s) 827, 1176, 1817, 2225, 2229, 2811
AcvI CACGTG 1 cut(s) 244
AcyI GRCGYC 1 cut(s) 45
AfaI GTAC 2 cut(s) 1071, 1830
AfeI AGCGCT 2 cut(s) 2634, 2721
AfiI CCNNNNNNNGG 2 cut(s) 1705, 2169
AflIII ACRYGT 3 cut(s) 241, 1914, 2517
AhdI GACNNNNNGTC 1 cut(s) 478
AhlI ACTAGT 1 cut(s) 3067
AjnI CCWGG 7 cut(s) 986, 1366, 1574, 1753, 1831, 1905, 3075
AjuI GAANNNNNNNTTGG 6 cut(s) 130, 162, 1172, 1204, 1459, 1491
Alw21I GWGCWC 3 cut(s) 17, 384, 2361
Alw26I GTCTC 5 cut(s) 172, 372, 1636, 2151, 2618
AlwI GGATC 4 cut(s) 48, 420, 2180, 2389
AlwNI CAGNNNCTG 2 cut(s) 2384, 2396
Ama87I CYCGRG 2 cut(s) 633, 2705
Aor13HI TCCGGA 2 cut(s) 1295, 2911
Aor51HI AGCGCT 2 cut(s) 2634, 2721
AoxI GGCC 2 cut(s) 2446, 3073
ApoI RAATTY 9 cut(s) 101, 500, 1116, 1213, 1310, 1421, 1993, 2065, 3057
AseI ATTAAT 2 cut(s) 1023, 1493
Asp700I GAANNNNTTC 3 cut(s) 1821, 1983, 2793
AspLEI GCGC 2 cut(s) 2635, 2722
AspS9I GGNCC 2 cut(s) 306, 2263
AsuHPI GGTGA 5 cut(s) 863, 886, 1774, 1881, 1941
AvaI CYCGRG 2 cut(s) 633, 2705
AvaII GGWCC 2 cut(s) 306, 2263
AxyI CCTNAGG 1 cut(s) 116
BaeGI GKGCMC 1 cut(s) 2116
BaeI ACNNNNGTAYC 2 cut(s) 2802, 2835
BanI GGYRCC 1 cut(s) 2111
BanII GRGCYC 2 cut(s) 17, 2361
BarI GAAGNNNNNNTAC 2 cut(s) 2252, 2284
BbrPI CACGTG 1 cut(s) 244
BbsI GAAGAC 5 cut(s) 48, 998, 2047, 2204, 2235
Bbv12I GWGCWC 3 cut(s) 17, 384, 2361
BbvCI CCTCAGC 1 cut(s) 939
BccI CCATC 4 cut(s) 1711, 2228, 2909, 2963
BceAI ACGGC 1 cut(s) 2549
BciT130I CCWGG 7 cut(s) 988, 1368, 1576, 1755, 1833, 1907, 3077
BciVI GTATCC 3 cut(s) 1059, 2567, 2815
BcoDI GTCTC 5 cut(s) 172, 372, 1636, 2151, 2618
BcuI ACTAGT 1 cut(s) 3067
BfaI CTAG 8 cut(s) 260, 284, 1253, 1290, 1464, 1964, 2942, 3068
BfmI CTRYAG 4 cut(s) 573, 2164, 2388, 2628
BfoI RGCGCY 2 cut(s) 2636, 2723
BfuI GTATCC 3 cut(s) 1059, 2567, 2815
BglII AGATCT 2 cut(s) 2087, 2490
BlpI GCTNAGC 1 cut(s) 1749
Bme1390I CCNGG 7 cut(s) 988, 1368, 1576, 1755, 1833, 1907, 3077
Bme18I GGWCC 2 cut(s) 306, 2263
BmeRI GACNNNNNGTC 1 cut(s) 478
BmeT110I CYCGRG 2 cut(s) 633, 2705
BmgT120I GGNCC 2 cut(s) 306, 2263
BmiI GGNNCC 2 cut(s) 307, 2113
BmrFI CCNGG 7 cut(s) 988, 1368, 1576, 1755, 1833, 1907, 3077
BmrI ACTGGG 1 cut(s) 2974
BmsI GCATC 6 cut(s) 160, 592, 1101, 1193, 1817, 2336
BmuI ACTGGG 1 cut(s) 2974
BpiI GAAGAC 5 cut(s) 48, 998, 2047, 2204, 2235
BplI GAGNNNNNCTC 2 cut(s) 3025, 3057
BpmI CTGGAG 2 cut(s) 1389, 1575
Bpu10I CCTNAGC 1 cut(s) 939
Bpu1102I GCTNAGC 1 cut(s) 1749
BpuEI CTTGAG 4 cut(s) 354, 689, 1206, 1694
Bsa29I ATCGAT 1 cut(s) 2190
BsaAI YACGTR 1 cut(s) 244
BsaHI GRCGYC 1 cut(s) 45
BsaI GGTCTC 1 cut(s) 1636
BsaJI CCNNGG 4 cut(s) 57, 301, 309, 1754
BsaWI WCCGGW 2 cut(s) 1295, 2911
Bsc4I CCNNNNNNNGG 2 cut(s) 1705, 2169
Bse1I ACTGG 5 cut(s) 75, 214, 1952, 2596, 2980
Bse21I CCTNAGG 1 cut(s) 116
Bse3DI GCAATG 3 cut(s) 1135, 1368, 2605
BseAI TCCGGA 2 cut(s) 1295, 2911
BseBI CCWGG 7 cut(s) 988, 1368, 1576, 1755, 1833, 1907, 3077
BseCI ATCGAT 1 cut(s) 2190
BseDI CCNNGG 4 cut(s) 57, 301, 309, 1754
BseGI GGATG 7 cut(s) 67, 151, 211, 1092, 1339, 1687, 2920
BseLI CCNNNNNNNGG 2 cut(s) 1705, 2169
BseMI GCAATG 3 cut(s) 1135, 1368, 2605
BseMII CTCAG 6 cut(s) 410, 456, 930, 1740, 1955, 2679
BseNI ACTGG 5 cut(s) 75, 214, 1952, 2596, 2980
BseRI GAGGAG 1 cut(s) 203
BseSI GKGCMC 1 cut(s) 2116
BseYI CCCAGC 1 cut(s) 1704
BsgI GTGCAG 1 cut(s) 954
BshFI GGCC 2 cut(s) 2448, 3075
BshNI GGYRCC 1 cut(s) 2111
BshVI ATCGAT 1 cut(s) 2190
BsiHKAI GWGCWC 3 cut(s) 17, 384, 2361
BsiHKCI CYCGRG 2 cut(s) 633, 2705
BsiSI CCGG 4 cut(s) 1296, 2170, 2621, 2912
BslFI GGGAC 4 cut(s) 319, 689, 1771, 2962
BslI CCNNNNNNNGG 2 cut(s) 1705, 2169
BsmAI GTCTC 5 cut(s) 172, 372, 1636, 2151, 2618
BsmFI GGGAC 4 cut(s) 319, 689, 1771, 2962
BsmI GAATGC 2 cut(s) 1609, 2420
BsnI GGCC 2 cut(s) 2448, 3075
Bso31I GGTCTC 1 cut(s) 1636
BsoBI CYCGRG 2 cut(s) 633, 2705
Bsp1286I GDGCHC 4 cut(s) 17, 384, 2116, 2361
Bsp13I TCCGGA 2 cut(s) 1295, 2911
Bsp1720I GCTNAGC 1 cut(s) 1749
BspACI CCGC 5 cut(s) 1149, 1152, 1741, 2266, 2880
BspANI GGCC 2 cut(s) 2448, 3075
BspCNI CTCAG 6 cut(s) 411, 455, 931, 1741, 1954, 2680
BspDI ATCGAT 1 cut(s) 2190
BspEI TCCGGA 2 cut(s) 1295, 2911
BspLI GGNNCC 2 cut(s) 307, 2113
BspMAI CTGCAG 2 cut(s) 2392, 2632
BspPI GGATC 4 cut(s) 48, 420, 2180, 2389
BspQI GCTCTTC 1 cut(s) 372
BspT107I GGYRCC 1 cut(s) 2111
BspTNI GGTCTC 1 cut(s) 1636
BsrDI GCAATG 3 cut(s) 1135, 1368, 2605
BsrI ACTGG 5 cut(s) 75, 214, 1952, 2596, 2980
BssECI CCNNGG 4 cut(s) 57, 301, 309, 1754
BssNAI GTATAC 2 cut(s) 195, 741
BssNI GRCGYC 1 cut(s) 45
BssT1I CCWWGG 2 cut(s) 301, 309
Bst1107I GTATAC 2 cut(s) 195, 741
Bst2UI CCWGG 7 cut(s) 988, 1368, 1576, 1755, 1833, 1907, 3077
Bst4CI ACNGT 8 cut(s) 516, 730, 1140, 1762, 1893, 2273, 2671, 2855
Bst6I CTCTTC 3 cut(s) 372, 1497, 1639
BstACI GRCGYC 1 cut(s) 45
BstAPI GCANNNNNTGC 2 cut(s) 157, 2450
BstBAI YACGTR 1 cut(s) 244
BstC8I GCNNGC 3 cut(s) 1871, 2446, 2763
BstDEI CTNAG 9 cut(s) 116, 419, 442, 505, 939, 1409, 1749, 1941, 2688
BstF5I GGATG 7 cut(s) 67, 151, 211, 1092, 1339, 1687, 2920
BstH2I RGCGCY 2 cut(s) 2636, 2723
BstHHI GCGC 2 cut(s) 2635, 2722
BstMAI GTCTC 5 cut(s) 172, 372, 1636, 2151, 2618
BstMWI GCNNNNNNNGC 8 cut(s) 21, 157, 941, 2132, 2450, 2564, 2758, 3042
BstNI CCWGG 7 cut(s) 988, 1368, 1576, 1755, 1833, 1907, 3077
BstNSI RCATGY 4 cut(s) 223, 1873, 1918, 2521
BstSCI CCNGG 7 cut(s) 986, 1366, 1574, 1753, 1831, 1905, 3075
BstSFI CTRYAG 4 cut(s) 573, 2164, 2388, 2628
BstSLI GKGCMC 1 cut(s) 2116
BstV2I GAAGAC 5 cut(s) 48, 998, 2047, 2204, 2235
BstX2I RGATCY 5 cut(s) 53, 412, 2087, 2381, 2490
BstYI RGATCY 5 cut(s) 53, 412, 2087, 2381, 2490
BstZ17I GTATAC 2 cut(s) 195, 741
Bsu15I ATCGAT 1 cut(s) 2190
Bsu36I CCTNAGG 1 cut(s) 116
BsuI GTATCC 3 cut(s) 1059, 2567, 2815
BsuRI GGCC 2 cut(s) 2448, 3075
BsuTUI ATCGAT 1 cut(s) 2190
BtsCI GGATG 7 cut(s) 67, 151, 211, 1092, 1339, 1687, 2920
BtsIMutI CAGTG 5 cut(s) 96, 221, 981, 1988, 2987
Cac8I GCNNGC 3 cut(s) 1871, 2446, 2763
CaiI CAGNNNCTG 2 cut(s) 2384, 2396
CfoI GCGC 2 cut(s) 2635, 2722
Cfr13I GGNCC 2 cut(s) 306, 2263
ClaI ATCGAT 1 cut(s) 2190
CseI GACGC 1 cut(s) 53
CsiI ACCWGGT 2 cut(s) 1831, 1905
Csp6I GTAC 2 cut(s) 1070, 1829
CviQI GTAC 2 cut(s) 1070, 1829
DdeI CTNAG 9 cut(s) 116, 419, 442, 505, 939, 1409, 1749, 1941, 2688
DraI TTTAAA 1 cut(s) 927
DrdI GACNNNNNNGTC 1 cut(s) 352
DriI GACNNNNNGTC 1 cut(s) 478
DseDI GACNNNNNNGTC 1 cut(s) 352
Eam1104I CTCTTC 3 cut(s) 372, 1497, 1639
Eam1105I GACNNNNNGTC 1 cut(s) 478
EarI CTCTTC 3 cut(s) 372, 1497, 1639
Ecl136II GAGCTC 2 cut(s) 15, 2359
Eco130I CCWWGG 2 cut(s) 301, 309
Eco147I AGGCCT 1 cut(s) 2448
Eco24I GRGCYC 2 cut(s) 17, 2361
Eco31I GGTCTC 1 cut(s) 1636
Eco32I GATATC 1 cut(s) 2188
Eco47I GGWCC 2 cut(s) 306, 2263
Eco47III AGCGCT 2 cut(s) 2634, 2721
Eco53kI GAGCTC 2 cut(s) 15, 2359
Eco57I CTGAAG 6 cut(s) 827, 1176, 1817, 2225, 2229, 2811
Eco72I CACGTG 1 cut(s) 244
Eco81I CCTNAGG 1 cut(s) 116
Eco88I CYCGRG 2 cut(s) 633, 2705
EcoICRI GAGCTC 2 cut(s) 15, 2359
EcoRI GAATTC 2 cut(s) 101, 1213
EcoRII CCWGG 7 cut(s) 986, 1366, 1574, 1753, 1831, 1905, 3075
EcoRV GATATC 1 cut(s) 2188
EcoT14I CCWWGG 2 cut(s) 301, 309
EcoT22I ATGCAT 2 cut(s) 1609, 2329
EcoT38I GRGCYC 2 cut(s) 17, 2361
ErhI CCWWGG 2 cut(s) 301, 309
FalI AAGNNNNNCTT 2 cut(s) 651, 683
FaqI GGGAC 4 cut(s) 319, 689, 1771, 2962
FauI CCCGC 1 cut(s) 2873
FauNDI CATATG 4 cut(s) 320, 628, 2457, 2641
FblI GTMKAC 4 cut(s) 194, 740, 838, 1902
FokI GGATG 7 cut(s) 74, 138, 218, 1079, 1346, 1674, 2927
FriOI GRGCYC 2 cut(s) 17, 2361
FspBI CTAG 8 cut(s) 260, 284, 1253, 1290, 1464, 1964, 2942, 3068
GlaI GCGC 2 cut(s) 2634, 2721
GsaI CCCAGC 1 cut(s) 1708
GsuI CTGGAG 2 cut(s) 1389, 1575
HaeII RGCGCY 2 cut(s) 2636, 2723
HaeIII GGCC 2 cut(s) 2448, 3075
HapII CCGG 4 cut(s) 1296, 2170, 2621, 2912
HgaI GACGC 1 cut(s) 53
HhaI GCGC 2 cut(s) 2635, 2722
Hin1I GRCGYC 1 cut(s) 45
Hin6I GCGC 2 cut(s) 2633, 2720
HinP1I GCGC 2 cut(s) 2633, 2720
HindIII AAGCTT 4 cut(s) 665, 1469, 2207, 2276
HpaII CCGG 4 cut(s) 1296, 2170, 2621, 2912
HphI GGTGA 5 cut(s) 863, 886, 1774, 1881, 1941
Hpy166II GTNNAC 8 cut(s) 195, 332, 741, 839, 1781, 1903, 1912, 2531
Hpy8I GTNNAC 8 cut(s) 195, 332, 741, 839, 1781, 1903, 1912, 2531
HpyAV CCTTC 8 cut(s) 692, 1679, 1973, 2167, 2254, 2704, 2920, 2950
HpyCH4III ACNGT 8 cut(s) 516, 730, 1140, 1762, 1893, 2273, 2671, 2855
HpyCH4IV ACGT 5 cut(s) 243, 587, 720, 1960, 2833
HpyF10VI GCNNNNNNNGC 8 cut(s) 21, 157, 941, 2132, 2450, 2564, 2758, 3042
HpyF3I CTNAG 9 cut(s) 116, 419, 442, 505, 939, 1409, 1749, 1941, 2688
HpySE526I ACGT 5 cut(s) 243, 587, 720, 1960, 2833
Hsp92I GRCGYC 1 cut(s) 45
HspAI GCGC 2 cut(s) 2633, 2720
Kpn2I TCCGGA 2 cut(s) 1295, 2911
LguI GCTCTTC 1 cut(s) 372
LmnI GCTCC 3 cut(s) 12, 1298, 3008
LweI GCATC 6 cut(s) 160, 592, 1101, 1193, 1817, 2336
MabI ACCWGGT 2 cut(s) 1831, 1905
MaeI CTAG 8 cut(s) 260, 284, 1253, 1290, 1464, 1964, 2942, 3068
MaeII ACGT 5 cut(s) 243, 587, 720, 1960, 2833
MaeIII GTNAC 6 cut(s) 583, 707, 976, 1206, 1947, 2834
MfeI CAATTG 1 cut(s) 2405
MflI RGATCY 5 cut(s) 53, 412, 2087, 2381, 2490
MhlI GDGCHC 4 cut(s) 17, 384, 2116, 2361
MlyI GAGTC 7 cut(s) 340, 374, 659, 959, 1633, 2153, 2194
MmeI TCCRAC 5 cut(s) 300, 570, 1170, 1311, 1638
Mph1103I ATGCAT 2 cut(s) 1609, 2329
MroI TCCGGA 2 cut(s) 1295, 2911
MroXI GAANNNNTTC 3 cut(s) 1821, 1983, 2793
MslI CAYNNNNRTG 4 cut(s) 974, 1097, 1625, 2322
MspA1I CMGCKG 2 cut(s) 938, 1154
MspI CCGG 4 cut(s) 1296, 2170, 2621, 2912
MspR9I CCNGG 7 cut(s) 988, 1368, 1576, 1755, 1833, 1907, 3077
MunI CAATTG 1 cut(s) 2405
Mva1269I GAATGC 2 cut(s) 1609, 2420
MvaI CCWGG 7 cut(s) 988, 1368, 1576, 1755, 1833, 1907, 3077
MwoI GCNNNNNNNGC 8 cut(s) 21, 157, 941, 2132, 2450, 2564, 2758, 3042
NdeI CATATG 4 cut(s) 320, 628, 2457, 2641
NlaIV GGNNCC 2 cut(s) 307, 2113
NmuCI GTSAC 4 cut(s) 583, 976, 1947, 2834
NsiI ATGCAT 2 cut(s) 1609, 2329
NspI RCATGY 4 cut(s) 223, 1873, 1918, 2521
PaeI GCATGC 1 cut(s) 1873
PaeR7I CTCGAG 2 cut(s) 633, 2705
PceI AGGCCT 1 cut(s) 2448
PciI ACATGT 2 cut(s) 1914, 2517
PciSI GCTCTTC 1 cut(s) 372
PctI GAATGC 2 cut(s) 1609, 2420
PdmI GAANNNNTTC 3 cut(s) 1821, 1983, 2793
PleI GAGTC 7 cut(s) 340, 373, 658, 959, 1633, 2152, 2194
PmaCI CACGTG 1 cut(s) 244
PmlI CACGTG 1 cut(s) 244
PpsI GAGTC 7 cut(s) 340, 373, 658, 959, 1633, 2152, 2194
Ppu21I YACGTR 1 cut(s) 244
PscI ACATGT 2 cut(s) 1914, 2517
PshBI ATTAAT 2 cut(s) 1023, 1493
PsiI TTATAA 1 cut(s) 734
Psp124BI GAGCTC 2 cut(s) 17, 2361
Psp6I CCWGG 7 cut(s) 986, 1366, 1574, 1753, 1831, 1905, 3075
PspCI CACGTG 1 cut(s) 244
PspFI CCCAGC 1 cut(s) 1704
PspGI CCWGG 7 cut(s) 986, 1366, 1574, 1753, 1831, 1905, 3075
PspN4I GGNNCC 2 cut(s) 307, 2113
PspPI GGNCC 2 cut(s) 306, 2263
PspXI VCTCGAGB 1 cut(s) 633
PstI CTGCAG 2 cut(s) 2392, 2632
PstNI CAGNNNCTG 2 cut(s) 2384, 2396
PsuI RGATCY 5 cut(s) 53, 412, 2087, 2381, 2490
PvuII CAGCTG 1 cut(s) 938
RsaI GTAC 2 cut(s) 1071, 1830
RsaNI GTAC 2 cut(s) 1070, 1829
RseI CAYNNNNRTG 4 cut(s) 974, 1097, 1625, 2322
SacI GAGCTC 2 cut(s) 17, 2361
SapI GCTCTTC 1 cut(s) 372
Sau96I GGNCC 2 cut(s) 306, 2263
SchI GAGTC 7 cut(s) 340, 374, 659, 959, 1633, 2153, 2194
ScrFI CCNGG 7 cut(s) 988, 1368, 1576, 1755, 1833, 1907, 3077
SduI GDGCHC 4 cut(s) 17, 384, 2116, 2361
SexAI ACCWGGT 2 cut(s) 1831, 1905
SfaNI GCATC 6 cut(s) 160, 592, 1101, 1193, 1817, 2336
SfcI CTRYAG 4 cut(s) 573, 2164, 2388, 2628
Sfr274I CTCGAG 2 cut(s) 633, 2705
SinI GGWCC 2 cut(s) 306, 2263
SlaI CTCGAG 2 cut(s) 633, 2705
SmiMI CAYNNNNRTG 4 cut(s) 974, 1097, 1625, 2322
SmlI CTYRAG 7 cut(s) 16, 369, 633, 668, 1221, 1673, 2705
SmoI CTYRAG 7 cut(s) 16, 369, 633, 668, 1221, 1673, 2705
SpeI ACTAGT 1 cut(s) 3067
SphI GCATGC 1 cut(s) 1873
SseBI AGGCCT 1 cut(s) 2448
SsiI CCGC 5 cut(s) 1149, 1152, 1741, 2266, 2880
SspMI CTAG 8 cut(s) 260, 284, 1253, 1290, 1464, 1964, 2942, 3068
SstI GAGCTC 2 cut(s) 17, 2361
StuI AGGCCT 1 cut(s) 2448
StyD4I CCNGG 7 cut(s) 986, 1366, 1574, 1753, 1831, 1905, 3075
StyI CCWWGG 2 cut(s) 301, 309
TaaI ACNGT 8 cut(s) 516, 730, 1140, 1762, 1893, 2273, 2671, 2855
TaiI ACGT 5 cut(s) 246, 590, 723, 1963, 2836
TaqI TCGA 8 cut(s) 176, 356, 634, 1637, 2190, 2361, 2706, 2850
TauI GCSGC 1 cut(s) 1154
TscAI CASTG 5 cut(s) 103, 221, 981, 1995, 2987
TseFI GTSAC 4 cut(s) 583, 976, 1947, 2834
Tsp45I GTSAC 4 cut(s) 583, 976, 1947, 2834
TspGWI ACGGA 2 cut(s) 1041, 2882
TspRI CASTG 5 cut(s) 103, 221, 981, 1995, 2987
VpaK11BI GGWCC 2 cut(s) 306, 2263
VspI ATTAAT 2 cut(s) 1023, 1493
XapI RAATTY 9 cut(s) 101, 500, 1116, 1213, 1310, 1421, 1993, 2065, 3057
XbaI TCTAGA 1 cut(s) 1252
XceI RCATGY 4 cut(s) 223, 1873, 1918, 2521
XhoI CTCGAG 2 cut(s) 633, 2705
XmiI GTMKAC 4 cut(s) 194, 740, 838, 1902
XmnI GAANNNNTTC 3 cut(s) 1821, 1983, 2793
XspI CTAG 8 cut(s) 260, 284, 1253, 1290, 1464, 1964, 2942, 3068
Zsp2I ATGCAT 2 cut(s) 1609, 2329
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.