pycom14g01500

Dehydration-responsive element-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0010581
Physical Location & Seq
Reverse (-)
1107554 .. 1108195
642 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g01500.1

Sequence Viewer

Length: 642 bp
ATGGAAAATTTAACCGATGATAGATCGTCTTCAACTTCGATTATTTCTGTTCCAACCCTAATGCAAACTAGTATGGGGGTGGTTAAAAAGAGAAAGGCAGGGAGGAAGAAGTTCACAGAGACTAGGCACCCAGTTTACAAAGGTGTGAGGCAAAGGTGTGGGAAGTGGGTGTGTGAGCTGAGGCAACCCGATCACAAGAAATCGCGGATATGGCTCGGAACTTTCACTAGCCCTGACATGGCTGCTAGGGCTTATGATGTTGCAGCCTTGGCTCTCAAGGGTGAGTCTGCTTCACTCAACTTTCCTAACGAGGCAAGTGCTTTGCCGCGCTTCGAGTCGAATGCTTATACCGTAAAGGACATACAATGTGCTGCCTTAGAGGCTGCCGAGGCATTCTTGGAGGTTAAAGGTAAAGCCTCTTCATCTTCTTCTTTGAAGTTGGAGAAGGTAGAGGAAGAAGAGGTGAGAAAAGTTGTGTATTTGGATGAGGAGGAGTTGTTCAACATGCCGGGGTTGATTGATAGCATGGCTGAGGGTTTGATCCTCACTCCACCATCCCTGCAAAAGGGTTTTAATTGGAACGAATATGACGACGACGACTGGGGGAATAATACTACTGACATTTCCTTGTGGAGTGACTGA

Protein Analysis

214

Amino Acids

23.85

Weight (kDa)

5.28

Isoelectric Point (pI)

43.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 44 - 94 8.4e-11 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 126
AccII CGCG 2 cut(s) 205, 328
AciI CCGC 2 cut(s) 205, 326
AclWI GGATC 1 cut(s) 535
AcsI RAATTY 1 cut(s) 7
AfiI CCNNNNNNNGG 2 cut(s) 238, 565
AgsI TTSAA 3 cut(s) 33, 436, 502
AhlI ACTAGT 1 cut(s) 68
AloI GAACNNNNNNTCC 2 cut(s) 482, 514
AluBI AGCT 1 cut(s) 178
AluI AGCT 1 cut(s) 178
Alw26I GTCTC 1 cut(s) 113
AlwI GGATC 1 cut(s) 535
ApeKI GCWGC 4 cut(s) 242, 263, 371, 383
ApoI RAATTY 1 cut(s) 7
Asp700I GAANNNNTTC 1 cut(s) 110
AspLEI GCGC 1 cut(s) 330
AsuC2I CCSGG 1 cut(s) 510
AsuHPI GGTGA 2 cut(s) 293, 475
BanI GGYRCC 1 cut(s) 126
BarI GAAGNNNNNNTAC 2 cut(s) 403, 435
BbsI GAAGAC 1 cut(s) 21
BbvCI CCTCAGC 2 cut(s) 179, 531
BbvI GCAGC 4 cut(s) 229, 275, 358, 370
BccI CCATC 1 cut(s) 562
BcgI CGANNNNNNTGC 4 cut(s) 299, 323, 333, 357
BcnI CCSGG 1 cut(s) 510
BcoDI GTCTC 1 cut(s) 113
BcuI ACTAGT 1 cut(s) 68
BfaI CTAG 4 cut(s) 69, 123, 228, 246
BglI GCCNNNNNGGC 1 cut(s) 380
BisI GCNGC 5 cut(s) 243, 264, 326, 372, 384
BlsI GCNGC 5 cut(s) 244, 265, 327, 373, 385
Bme1390I CCNGG 1 cut(s) 510
BmiI GGNNCC 1 cut(s) 128
BmrFI CCNGG 1 cut(s) 510
BmrI ACTGGG 2 cut(s) 125, 610
BmuI ACTGGG 2 cut(s) 125, 610
BpiI GAAGAC 1 cut(s) 21
Bpu10I CCTNAGC 2 cut(s) 179, 531
BpuEI CTTGAG 1 cut(s) 260
BpuMI CCSGG 1 cut(s) 510
BsaJI CCNNGG 3 cut(s) 267, 387, 509
BsaXI ACNNNNNCTCC 2 cut(s) 482, 512
Bsc4I CCNNNNNNNGG 2 cut(s) 238, 565
Bse1I ACTGG 2 cut(s) 131, 605
BseDI CCNNGG 3 cut(s) 267, 387, 509
BseGI GGATG 2 cut(s) 490, 554
BseLI CCNNNNNNNGG 2 cut(s) 238, 565
BseMII CTCAG 2 cut(s) 170, 522
BseNI ACTGG 2 cut(s) 131, 605
BseRI GAGGAG 2 cut(s) 503, 506
BseXI GCAGC 4 cut(s) 229, 275, 358, 370
Bsh1236I CGCG 2 cut(s) 205, 328
BshNI GGYRCC 1 cut(s) 126
BsiSI CCGG 1 cut(s) 509
BslI CCNNNNNNNGG 2 cut(s) 238, 565
BsmAI GTCTC 1 cut(s) 113
BsmI GAATGC 2 cut(s) 346, 392
Bsp143I GATC 3 cut(s) 23, 190, 540
BspACI CCGC 2 cut(s) 205, 326
BspCNI CTCAG 2 cut(s) 171, 523
BspFNI CGCG 2 cut(s) 205, 328
BspLI GGNNCC 1 cut(s) 128
BspPI GGATC 1 cut(s) 535
BspT107I GGYRCC 1 cut(s) 126
BsrI ACTGG 2 cut(s) 131, 605
BssECI CCNNGG 3 cut(s) 267, 387, 509
BssMI GATC 3 cut(s) 23, 190, 540
BssT1I CCWWGG 1 cut(s) 267
Bst4CI ACNGT 1 cut(s) 352
Bst6I CTCTTC 2 cut(s) 424, 453
BstDEI CTNAG 3 cut(s) 179, 376, 531
BstENI CCTNNNNNAGG 1 cut(s) 563
BstF5I GGATG 2 cut(s) 490, 554
BstFNI CGCG 2 cut(s) 205, 328
BstHHI GCGC 1 cut(s) 330
BstKTI GATC 3 cut(s) 26, 193, 543
BstMAI GTCTC 1 cut(s) 113
BstMBI GATC 3 cut(s) 23, 190, 540
BstMWI GCNNNNNNNGC 5 cut(s) 211, 248, 269, 380, 389
BstNSI RCATGY 1 cut(s) 508
BstSCI CCNGG 1 cut(s) 508
BstUI CGCG 2 cut(s) 205, 328
BstV1I GCAGC 4 cut(s) 229, 275, 358, 370
BstV2I GAAGAC 1 cut(s) 21
BtsCI GGATG 2 cut(s) 490, 554
CfoI GCGC 1 cut(s) 330
CviAII CATG 3 cut(s) 238, 505, 526
DdeI CTNAG 3 cut(s) 179, 376, 531
DpnI GATC 3 cut(s) 25, 192, 542
DpnII GATC 3 cut(s) 23, 190, 540
Eam1104I CTCTTC 2 cut(s) 424, 453
EarI CTCTTC 2 cut(s) 424, 453
Eco130I CCWWGG 1 cut(s) 267
EcoNI CCTNNNNNAGG 1 cut(s) 563
EcoT14I CCWWGG 1 cut(s) 267
ErhI CCWWGG 1 cut(s) 267
FaeI CATG 3 cut(s) 241, 508, 529
FaiI YATR 9 cut(s) 74, 211, 239, 255, 348, 362, 506, 527, 588
FatI CATG 3 cut(s) 237, 504, 525
Fnu4HI GCNGC 5 cut(s) 243, 264, 326, 372, 384
FokI GGATG 2 cut(s) 497, 541
Fsp4HI GCNGC 5 cut(s) 243, 264, 326, 372, 384
FspBI CTAG 4 cut(s) 69, 123, 228, 246
GlaI GCGC 1 cut(s) 329
GluI GCNGC 5 cut(s) 243, 264, 326, 372, 384
HapII CCGG 1 cut(s) 509
HhaI GCGC 1 cut(s) 330
Hin1II CATG 3 cut(s) 241, 508, 529
Hin6I GCGC 1 cut(s) 328
HinP1I GCGC 1 cut(s) 328
HinfI GANTC 2 cut(s) 284, 335
HpaII CCGG 1 cut(s) 509
HphI GGTGA 2 cut(s) 293, 475
Hpy166II GTNNAC 2 cut(s) 114, 136
Hpy188I TCNGA 1 cut(s) 218
Hpy8I GTNNAC 2 cut(s) 114, 136
Hpy99I CGWCG 2 cut(s) 596, 599
HpyAV CCTTC 1 cut(s) 439
HpyCH4III ACNGT 1 cut(s) 352
HpyCH4V TGCA 3 cut(s) 64, 263, 562
HpyF10VI GCNNNNNNNGC 5 cut(s) 211, 248, 269, 380, 389
HpyF3I CTNAG 3 cut(s) 179, 376, 531
Hsp92II CATG 3 cut(s) 241, 508, 529
HspAI GCGC 1 cut(s) 328
Kzo9I GATC 3 cut(s) 23, 190, 540
LpnPI CCDG 6 cut(s) 84, 144, 246, 522, 572, 586
Lsp1109I GCAGC 4 cut(s) 229, 275, 358, 370
MaeI CTAG 4 cut(s) 69, 123, 228, 246
MaeIII GTNAC 1 cut(s) 635
MalI GATC 3 cut(s) 25, 192, 542
MboI GATC 3 cut(s) 23, 190, 540
MboII GAAGA 7 cut(s) 21, 118, 411, 417, 420, 467, 470
MluCI AATT 2 cut(s) 7, 574
MlyI GAGTC 2 cut(s) 293, 344
MmeI TCCRAC 2 cut(s) 77, 420
MroXI GAANNNNTTC 1 cut(s) 110
MseI TTAA 4 cut(s) 11, 84, 405, 573
MspI CCGG 1 cut(s) 509
MspR9I CCNGG 1 cut(s) 510
Mva1269I GAATGC 2 cut(s) 346, 392
MvnI CGCG 2 cut(s) 205, 328
MwoI GCNNNNNNNGC 5 cut(s) 211, 248, 269, 380, 389
NciI CCSGG 1 cut(s) 510
NdeII GATC 3 cut(s) 23, 190, 540
NlaIII CATG 3 cut(s) 241, 508, 529
NlaIV GGNNCC 1 cut(s) 128
NmeAIII GCCGAG 1 cut(s) 412
NmuCI GTSAC 1 cut(s) 635
NspI RCATGY 1 cut(s) 508
PcsI WCGNNNNNNNCGW 1 cut(s) 588
PctI GAATGC 2 cut(s) 346, 392
PdmI GAANNNNTTC 1 cut(s) 110
PkrI GCNGC 5 cut(s) 244, 265, 327, 373, 385
PleI GAGTC 2 cut(s) 292, 343
PpsI GAGTC 2 cut(s) 292, 343
PspN4I GGNNCC 1 cut(s) 128
SaqAI TTAA 4 cut(s) 11, 84, 405, 573
SatI GCNGC 5 cut(s) 243, 264, 326, 372, 384
Sau3AI GATC 3 cut(s) 23, 190, 540
SchI GAGTC 2 cut(s) 293, 344
ScrFI CCNGG 1 cut(s) 510
SetI ASST 7 cut(s) 145, 158, 180, 405, 412, 450, 465
SmlI CTYRAG 1 cut(s) 275
SmoI CTYRAG 1 cut(s) 275
SpeI ACTAGT 1 cut(s) 68
Sse9I AATT 2 cut(s) 7, 574
SsiI CCGC 2 cut(s) 205, 326
SspMI CTAG 4 cut(s) 69, 123, 228, 246
StyD4I CCNGG 1 cut(s) 508
StyI CCWWGG 1 cut(s) 267
TaaI ACNGT 1 cut(s) 352
TaqI TCGA 3 cut(s) 38, 333, 338
TasI AATT 2 cut(s) 7, 574
TauI GCSGC 1 cut(s) 328
Tru1I TTAA 4 cut(s) 11, 84, 405, 573
Tru9I TTAA 4 cut(s) 11, 84, 405, 573
TseFI GTSAC 1 cut(s) 635
TseI GCWGC 4 cut(s) 242, 263, 371, 383
Tsp45I GTSAC 1 cut(s) 635
TspDTI ATGAA 1 cut(s) 411
XagI CCTNNNNNAGG 1 cut(s) 563
XapI RAATTY 1 cut(s) 7
XceI RCATGY 1 cut(s) 508
XmnI GAANNNNTTC 1 cut(s) 110
XspI CTAG 4 cut(s) 69, 123, 228, 246
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.