pycom14g13600

ubiquitin-like-specific protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0005644
Physical Location & Seq
Forward (+)
16494920 .. 16496894
1975 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1005 bp
ATGTCACGGACGAGAAAGTCCAACACAAATAAAAAACCCACCACCGTCGAAATCGTCCTCGGCTCCTCCGACACGGACACCGTCACTGACTCCGACTCCGACTCCTCCGACCTACACTCTCCAATCTCAGAGCTTTTCAGACACAATGTTTCGAAACATCGCTCGTGCTGGAGGCATGTTTTTTCGTCTTCGATTGTTCAAAAGAAGAGAATAACTCTAGGGGATATTAAAGAGGTAAAGAAGAGTTCCATGTGCCTTTTGGAATGCCCTCCCCGCTTCACTCGCCGCGAGAGGTCAAAGAGATTTGGGCAAAAAGAAGAGTTGAAAGAGCTGATGAGGCCTCACAAGGTTGCTGAGCGTGCAGTTTCACGAAAAAGGGAAAAGCTAGACTCTGGAGCTTTCATGCGTTACTTTGAGAACTTGTGGAACAGCTTTTCAGAAGACAAGAGGACCTCTTTCGCTTACCTTGACTGTTTATGGTTTAGCTTGTACTTGCAACAAAACATTCACAAAGAACTTAAACGAGACATTAAGACCAAGGTGCTGAAGTGGATTAAGAATAAGTGCATCTTCTCAAAGAAATATGTCATTGTTCCTATCGTTTGCTGGAGCCACTGGAACCTCCTGATTCTATGCCATTTTGGTGAGAGTCTGCAGTCGGAAACCCAAAGACCATGTATGCTGTTGCTTGATTCTCTTCAAGGTGCTGATCCAGGGCGGTATGAAAAATATATAAGAAAATTTGTACTGGACATTTTTATGGAAGAGGGAAGACCTGAGAAGAAAGATCTTATCTACCGAATTCCTTTGTTGGTGCCTGAGGTGCCACAACAGAGAGATGATGTCGAGTGTGGTAACTTTGTTCTATACTACATTAACTTGTTCATGGAAGGTGCTCCCGAGAATTTCAGCACTGAGGGCTACCCCTACTTTATGAAGAAGGATTGGTTTTCCCCCAAGGGCTTGGATTATTTCTGCCAGAAACTGTACTCTTCTGCAACGTGA

Protein Analysis

335

Amino Acids

39.47

Weight (kDa)

9.23

Isoelectric Point (pI)

55.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C48 PF02902 135 - 315 2.4e-12 Ulp1 protease family, C-terminal catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 16
AccB1I GGYRCC 2 cut(s) 814, 823
AccII CGCG 1 cut(s) 288
AciI CCGC 3 cut(s) 274, 286, 718
AclWI GGATC 1 cut(s) 704
AcsI RAATTY 3 cut(s) 740, 801, 904
AcuI CTGAAG 1 cut(s) 566
AfaI GTAC 3 cut(s) 491, 747, 989
AgsI TTSAA 3 cut(s) 200, 325, 701
AjnI CCWGG 1 cut(s) 712
AluBI AGCT 6 cut(s) 133, 331, 385, 398, 432, 486
AluI AGCT 6 cut(s) 133, 331, 385, 398, 432, 486
Alw21I GWGCWC 1 cut(s) 898
Alw26I GTCTC 1 cut(s) 519
AlwI GGATC 1 cut(s) 704
AlwNI CAGNNNCTG 1 cut(s) 985
Ama87I CYCGRG 1 cut(s) 899
AoxI GGCC 1 cut(s) 338
ApoI RAATTY 3 cut(s) 740, 801, 904
AspS9I GGNCC 1 cut(s) 450
AsuHPI GGTGA 1 cut(s) 656
AsuII TTCGAA 1 cut(s) 152
AvaI CYCGRG 1 cut(s) 899
AvaII GGWCC 1 cut(s) 450
AxyI CCTNAGG 1 cut(s) 819
BanI GGYRCC 2 cut(s) 814, 823
BauI CACGAG 1 cut(s) 163
BbsI GAAGAC 3 cut(s) 180, 447, 778
Bbv12I GWGCWC 1 cut(s) 898
BciT130I CCWGG 1 cut(s) 714
BcoDI GTCTC 1 cut(s) 519
BfaI CTAG 2 cut(s) 218, 386
BfmI CTRYAG 1 cut(s) 653
BglII AGATCT 1 cut(s) 787
BisI GCNGC 1 cut(s) 286
BlpI GCTNAGC 1 cut(s) 354
BlsI GCNGC 1 cut(s) 287
Bme1390I CCNGG 1 cut(s) 714
Bme18I GGWCC 1 cut(s) 450
BmeT110I CYCGRG 1 cut(s) 899
BmgT120I GGNCC 1 cut(s) 450
BmiI GGNNCC 5 cut(s) 64, 611, 620, 816, 825
BmrFI CCNGG 1 cut(s) 714
BmsI GCATC 1 cut(s) 576
BpiI GAAGAC 3 cut(s) 180, 447, 778
BplI GAGNNNNNCTC 2 cut(s) 199, 231
BpmI CTGGAG 3 cut(s) 190, 414, 628
Bpu1102I GCTNAGC 1 cut(s) 354
Bpu14I TTCGAA 1 cut(s) 152
BsaJI CCNNGG 4 cut(s) 58, 537, 713, 957
BsaXI ACNNNNNCTCC 4 cut(s) 80, 86, 110, 116
Bse1I ACTGG 2 cut(s) 620, 753
Bse21I CCTNAGG 1 cut(s) 819
BseBI CCWGG 1 cut(s) 714
BseDI CCNNGG 4 cut(s) 58, 537, 713, 957
BseMII CTCAG 5 cut(s) 141, 345, 768, 810, 906
BseNI ACTGG 2 cut(s) 620, 753
BseRI GAGGAG 2 cut(s) 55, 94
BsgI GTGCAG 1 cut(s) 381
Bsh1236I CGCG 1 cut(s) 288
BshFI GGCC 1 cut(s) 340
BshNI GGYRCC 2 cut(s) 814, 823
BsiHKAI GWGCWC 1 cut(s) 898
BsiHKCI CYCGRG 1 cut(s) 899
BsmAI GTCTC 1 cut(s) 519
BsmI GAATGC 1 cut(s) 269
BsnI GGCC 1 cut(s) 340
BsoBI CYCGRG 1 cut(s) 899
Bsp119I TTCGAA 1 cut(s) 152
Bsp1286I GDGCHC 1 cut(s) 898
Bsp143I GATC 2 cut(s) 709, 787
Bsp1720I GCTNAGC 1 cut(s) 354
BspACI CCGC 3 cut(s) 274, 286, 718
BspANI GGCC 1 cut(s) 340
BspCNI CTCAG 5 cut(s) 140, 346, 769, 811, 907
BspFNI CGCG 1 cut(s) 288
BspLI GGNNCC 5 cut(s) 64, 611, 620, 816, 825
BspMAI CTGCAG 1 cut(s) 657
BspPI GGATC 1 cut(s) 704
BspT104I TTCGAA 1 cut(s) 152
BspT107I GGYRCC 2 cut(s) 814, 823
BsrI ACTGG 2 cut(s) 620, 753
BssECI CCNNGG 4 cut(s) 58, 537, 713, 957
BssMI GATC 2 cut(s) 709, 787
BssSI CACGAG 1 cut(s) 163
BssT1I CCWWGG 2 cut(s) 537, 957
Bst2BI CACGAG 1 cut(s) 163
Bst2UI CCWGG 1 cut(s) 714
Bst4CI ACNGT 4 cut(s) 46, 82, 473, 987
Bst6I CTCTTC 6 cut(s) 200, 236, 312, 702, 759, 997
BstBI TTCGAA 1 cut(s) 152
BstC8I GCNNGC 1 cut(s) 360
BstDEI CTNAG 5 cut(s) 127, 354, 777, 819, 915
BstFNI CGCG 1 cut(s) 288
BstKTI GATC 2 cut(s) 712, 790
BstMAI GTCTC 1 cut(s) 519
BstMBI GATC 2 cut(s) 709, 787
BstMWI GCNNNNNNNGC 6 cut(s) 273, 282, 337, 359, 823, 918
BstNI CCWGG 1 cut(s) 714
BstNSI RCATGY 1 cut(s) 179
BstSCI CCNGG 1 cut(s) 712
BstSFI CTRYAG 1 cut(s) 653
BstUI CGCG 1 cut(s) 288
BstV2I GAAGAC 3 cut(s) 180, 447, 778
BstX2I RGATCY 1 cut(s) 787
BstXI CCANNNNNNTGG 1 cut(s) 964
BstYI RGATCY 1 cut(s) 787
Bsu36I CCTNAGG 1 cut(s) 819
BsuRI GGCC 1 cut(s) 340
BtgZI GCGATG 1 cut(s) 143
BtsIMutI CAGTG 3 cut(s) 84, 613, 912
Cac8I GCNNGC 1 cut(s) 360
CaiI CAGNNNCTG 1 cut(s) 985
Cfr13I GGNCC 1 cut(s) 450
Csp6I GTAC 3 cut(s) 490, 746, 988
CviAII CATG 5 cut(s) 176, 250, 403, 675, 886
CviQI GTAC 3 cut(s) 490, 746, 988
DdeI CTNAG 5 cut(s) 127, 354, 777, 819, 915
DpnI GATC 2 cut(s) 711, 789
DpnII GATC 2 cut(s) 709, 787
DrdI GACNNNNNNGTC 1 cut(s) 16
DseDI GACNNNNNNGTC 1 cut(s) 16
Eam1104I CTCTTC 6 cut(s) 200, 236, 312, 702, 759, 997
EarI CTCTTC 6 cut(s) 200, 236, 312, 702, 759, 997
Eco130I CCWWGG 2 cut(s) 537, 957
Eco147I AGGCCT 1 cut(s) 340
Eco47I GGWCC 1 cut(s) 450
Eco57I CTGAAG 1 cut(s) 566
Eco81I CCTNAGG 1 cut(s) 819
Eco88I CYCGRG 1 cut(s) 899
EcoO109I RGGNCCY 1 cut(s) 450
EcoRI GAATTC 1 cut(s) 801
EcoRII CCWGG 1 cut(s) 712
EcoT14I CCWWGG 2 cut(s) 537, 957
ErhI CCWWGG 2 cut(s) 537, 957
FaeI CATG 5 cut(s) 179, 253, 406, 678, 889
FalI AAGNNNNNCTT 2 cut(s) 554, 586
FatI CATG 5 cut(s) 175, 249, 402, 674, 885
FauI CCCGC 1 cut(s) 281
Fnu4HI GCNGC 1 cut(s) 286
Fsp4HI GCNGC 1 cut(s) 286
FspBI CTAG 2 cut(s) 218, 386
GluI GCNGC 1 cut(s) 286
GsuI CTGGAG 3 cut(s) 190, 414, 628
HaeIII GGCC 1 cut(s) 340
Hin1II CATG 5 cut(s) 179, 253, 406, 678, 889
HinfI GANTC 7 cut(s) 89, 95, 101, 389, 628, 649, 692
HphI GGTGA 1 cut(s) 656
Hpy188I TCNGA 8 cut(s) 70, 94, 100, 109, 130, 140, 439, 661
Hpy188III TCNNGA 4 cut(s) 369, 393, 625, 899
Hpy99I CGWCG 1 cut(s) 50
HpyAV CCTTC 2 cut(s) 884, 934
HpyCH4III ACNGT 4 cut(s) 46, 82, 473, 987
HpyCH4IV ACGT 1 cut(s) 1001
HpyCH4V TGCA 5 cut(s) 362, 496, 567, 655, 998
HpyF10VI GCNNNNNNNGC 6 cut(s) 273, 282, 337, 359, 823, 918
HpyF3I CTNAG 5 cut(s) 127, 354, 777, 819, 915
HpySE526I ACGT 1 cut(s) 1001
Hsp92II CATG 5 cut(s) 179, 253, 406, 678, 889
Kzo9I GATC 2 cut(s) 709, 787
LmnI GCTCC 4 cut(s) 68, 395, 609, 901
LweI GCATC 1 cut(s) 576
MaeI CTAG 2 cut(s) 218, 386
MaeII ACGT 1 cut(s) 1001
MaeIII GTNAC 4 cut(s) 3, 82, 407, 854
MalI GATC 2 cut(s) 711, 789
MboI GATC 2 cut(s) 709, 787
MflI RGATCY 1 cut(s) 787
MhlI GDGCHC 1 cut(s) 898
MluCI AATT 3 cut(s) 740, 801, 904
MlyI GAGTC 5 cut(s) 83, 89, 95, 383, 658
MmeI TCCRAC 6 cut(s) 45, 93, 117, 123, 132, 639
MseI TTAA 5 cut(s) 228, 519, 531, 555, 876
MslI CAYNNNNRTG 2 cut(s) 642, 758
MspR9I CCNGG 1 cut(s) 714
Mva1269I GAATGC 1 cut(s) 269
MvaI CCWGG 1 cut(s) 714
MvnI CGCG 1 cut(s) 288
MwoI GCNNNNNNNGC 6 cut(s) 273, 282, 337, 359, 823, 918
NdeII GATC 2 cut(s) 709, 787
NlaIII CATG 5 cut(s) 179, 253, 406, 678, 889
NlaIV GGNNCC 5 cut(s) 64, 611, 620, 816, 825
NmeAIII GCCGAG 1 cut(s) 39
NmuCI GTSAC 2 cut(s) 3, 82
NspI RCATGY 1 cut(s) 179
NspV TTCGAA 1 cut(s) 152
PceI AGGCCT 1 cut(s) 340
PcsI WCGNNNNNNNCGW 1 cut(s) 66
PctI GAATGC 1 cut(s) 269
PfeI GAWTC 2 cut(s) 628, 692
PflFI GACNNNGTC 1 cut(s) 80
PkrI GCNGC 1 cut(s) 287
PleI GAGTC 5 cut(s) 83, 89, 95, 383, 657
PpsI GAGTC 5 cut(s) 83, 89, 95, 383, 657
PpuMI RGGWCCY 1 cut(s) 450
Psp5II RGGWCCY 1 cut(s) 450
Psp6I CCWGG 1 cut(s) 712
PspGI CCWGG 1 cut(s) 712
PspN4I GGNNCC 5 cut(s) 64, 611, 620, 816, 825
PspPI GGNCC 1 cut(s) 450
PspPPI RGGWCCY 1 cut(s) 450
PstI CTGCAG 1 cut(s) 657
PstNI CAGNNNCTG 1 cut(s) 985
PsuI RGATCY 1 cut(s) 787
PsyI GACNNNGTC 1 cut(s) 80
RsaI GTAC 3 cut(s) 491, 747, 989
RsaNI GTAC 3 cut(s) 490, 746, 988
RseI CAYNNNNRTG 2 cut(s) 642, 758
SaqAI TTAA 5 cut(s) 228, 519, 531, 555, 876
SatI GCNGC 1 cut(s) 286
Sau3AI GATC 2 cut(s) 709, 787
Sau96I GGNCC 1 cut(s) 450
SchI GAGTC 5 cut(s) 83, 89, 95, 383, 658
ScrFI CCNGG 1 cut(s) 714
SduI GDGCHC 1 cut(s) 898
SfaNI GCATC 1 cut(s) 576
SfcI CTRYAG 1 cut(s) 653
SfuI TTCGAA 1 cut(s) 152
SinI GGWCC 1 cut(s) 450
SmiMI CAYNNNNRTG 2 cut(s) 642, 758
Sse9I AATT 3 cut(s) 740, 801, 904
SseBI AGGCCT 1 cut(s) 340
SsiI CCGC 3 cut(s) 274, 286, 718
SspMI CTAG 2 cut(s) 218, 386
StuI AGGCCT 1 cut(s) 340
StyD4I CCNGG 1 cut(s) 712
StyI CCWWGG 2 cut(s) 537, 957
TaaI ACNGT 4 cut(s) 46, 82, 473, 987
TaiI ACGT 1 cut(s) 1004
TaqI TCGA 4 cut(s) 48, 152, 191, 846
TasI AATT 3 cut(s) 740, 801, 904
TatI WGTACW 3 cut(s) 489, 745, 987
TauI GCSGC 1 cut(s) 288
TfiI GAWTC 2 cut(s) 628, 692
Tru1I TTAA 5 cut(s) 228, 519, 531, 555, 876
Tru9I TTAA 5 cut(s) 228, 519, 531, 555, 876
TscAI CASTG 3 cut(s) 91, 620, 919
TseFI GTSAC 2 cut(s) 3, 82
Tsp45I GTSAC 2 cut(s) 3, 82
TspDTI ATGAA 4 cut(s) 391, 738, 874, 950
TspGWI ACGGA 2 cut(s) 22, 89
TspRI CASTG 3 cut(s) 91, 620, 919
Tth111I GACNNNGTC 1 cut(s) 80
VpaK11BI GGWCC 1 cut(s) 450
XapI RAATTY 3 cut(s) 740, 801, 904
XceI RCATGY 1 cut(s) 179
XcmI CCANNNNNNNNNTGG 1 cut(s) 256
XspI CTAG 2 cut(s) 218, 386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.