pycom14g14400

amine oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0003041
Physical Location & Seq
Forward (+)
17129710 .. 17134426
4717 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2358 bp
ATGGCCTCAGCTTCGAAAAAAACGACGCCTTCCCGTTGCTTCCGCCCGGATTCGGTGGCTCTCGTTCCGCGCGAGGCGGCGCAGACTTCTTCCAACGCCGTTGTATCGGCCTCCGCCTTGCAAGATTGGACTGGCGTCGCCGGCGCCGAGGATCGGCGCGATGATCAGCGCCCCAAGAAGATCGCAATGGCGGCCTTGATTCCGGAACCTTCTGCCAATGCCTCCGCTACAGGCATCTCAATCATGCTTAGGCCTCAAACAAGGCATCCATTGGACCCTTTATCTGCTGCTGAAATCTCTGTGGCAGTGGCAACTGTCAGGGCAGCTGGAGCGACGCCTGAGGTTAGAGATAGTATGCGTTTTGTTGAAGTGGTTCTACTAGAACCAGATAAACATGTTGTTGCATTAGCGGATGCGTACTTTTTTCCACCTTTCCAACCATCATTACTTCCCAGAACCAAAGGTGGACCTATAATCCCAACCAAGCTCCCCCCTCGGCGAGCTCGACTTGTTGTTTACAACAAGAAGTCAAATGAGACGAGCACATGGATTGTTGAGCTGTCTGAAGTACATGCAGCTACTCGAGGTGGACATCACAGAGGAAAAGTAATATCATCGGAAGTTGTTCCAGATGTTCAGCCTCCCATGGATGCTGTGGAATATGCAGAATGTGAAGCAGTTGTGAAAGACTATCCTCCATTTAGAGAAGCTATGAAGAAGAGAGGTATTGAAGACATGGACCTTGTGATGGTTGATGCCTGGTGTGTCGGCTATCATAGTGAGGCTGATGCTCCTGGGCAAAGACTTGCTAAACCATTGATATTCTGTAGAACTGAGAGTGACTGCCCAATGGAAAATGGTTACGCACGCCCAGTTGAGGGCATCTATATTCTTGTTGATATGCAAAACATGGTGGTGATAAAGTTTGAAGACCGTAAGCTTGTTCCCCTACCTCCAGCTGATCCATTGAGGAACTATACTCCTGGAGAAACAAGAGGTGGTGTTGATCGAAGTGATGTGAAGCCTTTACAAATTATTCAGCCTGAAGGTCCAAGCTTTCGTGTTAATGGGTACTTTGTGGAGTGGCAGAAGTGGAATTTTCGTATTGGATTCACTCCCAGGGAGGGACTGGTTATATATTCTGTTGCATATGTTGACGGCAATAGAGGGCGAAGGCCTGTAGCCCATAGGTTGAGTTTTGTGGAAATGGTGGTGCCATATGGAGATCCTAATGATCCACATTATCGAAAAAATGCTTTTGATGCAGGTGAAGATGGCCTTGGTAAAAATGCACATTCTCTAAAGAAGGGGTGTGATTGTTTGGGCTTAATCAAATACTTTGATGCACACTTTACGAACTTCACTGGAGGTGTTGAAAATATCGAAAATTGTGTATGTTTGCACGAAGAAGATCATGGAATTCTGTGGAAGCATCAGGACTGGAGAACAGGCTTAGCAGAAGTGCGCAGGTCAAGAAGGCTAACAGTGTCATTTGTATGTACTGTGGCTAATTATGAGTATGGGTTCTTTTGGCACTTTTATCAGGATGGAAAAATTGAAGCTGAAGTTAAACTTACAGGAGTTCTCAGCTTGGGAGCACTACAACCTGGAGAAGTTAGAAAATATGGAACTGTGATTGCACCCGGGCTATATGCACCAGTCCATCAACACTTTTTTGTTGCTCGTATGGATATGGCTGTTGATTGCAAGCCTGGGGAAACTTTCAATCAGGTTGTGGAGATGGATGTTAAGGTTGAGAAACCTGGAGAGAATAATGTCCACAGCAATGCATTTTATGCTGAGGAGACGCTTCTCAGGACTGAATCAGAGGCAATGCGTGACTGTAATCCTTTGACAGCTCGCCATTGGATTGTACGGAACACACGAACTGTTAATAGGACTGGACAGTTAACAGGCTACAAGCTCGTACCGGGTTCAAACTGTTTGCCATTAGCTGGTCCCGAGGCCAAGTTTTTGCGAAGAGCAGCTTTCCTGAAGCATAATCTATGGGTGACACAATATTCACGTGATGAAATGTTTCCCGGAGGAGAGTTTCCAAATCAAAATCCACGTGCTGGCGAAGGATTGGCCACTTGGGTTAAGAAAAATCGATCTCTGGAAGAAACTGACATAGTTCTTTGGTATGTATTCGGAATCACACACGTACCACGGTTGGAAGACTGGCCTGTCATGCCGGTGGAGCGCATTGGATTTATGCTCATGCCTCACGGATTCTTCAACGCCTCTCCGGCAGTGGATGTTCCACCAAGTGCATGTGAGTTGGAAGCGAAGGAAAATGATGTCAAAGACAGCGGCGTAGCCAAGCCCGTTCAGAACGGGTTGCTAGCAAAGCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00350 ko00360 ko00410 ko00950 ko00960 ko01100 ko01110 map00260 map00350 map00360 map00410 map00950 map00960 map01100 map01110
Pfam Domains
Protein Families
Cu_amine_oxid Cu_amine_oxidN2 Cu_amine_oxidN3

Protein Analysis

786

Amino Acids

87.52

Weight (kDa)

6.62

Isoelectric Point (pI)

39.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_amine_oxidN3 PF02728 214 - 317 1.6e-28 Copper amine oxidase, N3 domain
Cu_amine_oxid PF01179 343 - 751 3e-154 Copper amine oxidase, enzyme domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1256
AasI GACNNNNNNGTC 1 cut(s) 2186
Acc16I TGCGCA 1 cut(s) 1466
Acc36I ACCTGC 2 cut(s) 1256, 1458
AccB1I GGYRCC 2 cut(s) 143, 1213
AccB7I CCANNNNNTGG 2 cut(s) 1955, 2075
AccII CGCG 3 cut(s) 70, 72, 159
AccIII TCCGGA 1 cut(s) 202
AciI CCGC 8 cut(s) 43, 68, 77, 114, 191, 225, 410, 2313
AclWI GGATC 4 cut(s) 159, 956, 1220, 1229
AcoI YGGCCR 1 cut(s) 2088
AcsI RAATTY 2 cut(s) 1096, 1419
AcuI CTGAAG 4 cut(s) 585, 1065, 1584, 2015
AcvI CACGTG 2 cut(s) 2027, 2072
AcyI GRCGYC 4 cut(s) 26, 135, 144, 335
AdeI CACNNNGTG 1 cut(s) 2270
AfaI GTAC 7 cut(s) 419, 570, 1073, 1501, 1875, 1929, 2166
AfiI CCNNNNNNNGG 8 cut(s) 52, 153, 748, 877, 878, 1124, 1955, 2075
AflIII ACRYGT 2 cut(s) 394, 2161
AgsI TTSAA 8 cut(s) 368, 731, 929, 1376, 1559, 1726, 1938, 2239
AjnI CCWGG 7 cut(s) 758, 793, 982, 1118, 1606, 1711, 1762
AjuI GAANNNNNNNTTGG 2 cut(s) 1263, 1295
Alw21I GWGCWC 3 cut(s) 505, 545, 1600
Alw26I GTCTC 2 cut(s) 530, 1799
AlwI GGATC 4 cut(s) 159, 956, 1220, 1229
Ama87I CYCGRG 3 cut(s) 582, 1643, 1961
Aor13HI TCCGGA 1 cut(s) 202
AoxI GGCC 9 cut(s) 3, 108, 192, 251, 1175, 1276, 1965, 2088, 2183
ApeKI GCWGC 4 cut(s) 287, 323, 575, 1985
ApoI RAATTY 2 cut(s) 1096, 1419
Asp700I GAANNNNTTC 3 cut(s) 372, 624, 2037
AspLEI GCGC 7 cut(s) 72, 82, 146, 159, 171, 1467, 2205
AspS9I GGNCC 5 cut(s) 274, 467, 739, 1049, 1958
AsuC2I CCSGG 5 cut(s) 47, 1644, 1645, 1932, 2043
AsuHPI GGTGA 3 cut(s) 928, 1280, 2023
AsuII TTCGAA 1 cut(s) 14
AsuNHI GCTAGC 1 cut(s) 2344
AvaI CYCGRG 3 cut(s) 582, 1643, 1961
AvaII GGWCC 5 cut(s) 274, 467, 739, 1049, 1958
AxyI CCTNAGG 1 cut(s) 339
BaeI ACNNNNGTAYC 2 cut(s) 2148, 2181
BalI TGGCCA 1 cut(s) 2090
BanI GGYRCC 2 cut(s) 143, 1213
BanII GRGCYC 1 cut(s) 505
BarI GAAGNNNNNNTAC 2 cut(s) 360, 392
BbrPI CACGTG 2 cut(s) 2027, 2072
BbsI GAAGAC 3 cut(s) 738, 936, 2184
Bbv12I GWGCWC 3 cut(s) 505, 545, 1600
BbvCI CCTCAGC 2 cut(s) 7, 1800
BbvI GCAGC 4 cut(s) 274, 335, 587, 1997
BccI CCATC 6 cut(s) 448, 742, 1268, 1541, 1671, 1735
BceAI ACGGC 2 cut(s) 83, 1174
BciT130I CCWGG 7 cut(s) 760, 795, 984, 1120, 1608, 1713, 1764
BclI TGATCA 1 cut(s) 163
BcnI CCSGG 5 cut(s) 47, 1644, 1645, 1932, 2043
BcoDI GTCTC 2 cut(s) 530, 1799
BfaI CTAG 2 cut(s) 380, 2345
BfmI CTRYAG 3 cut(s) 228, 826, 1179
BfoI RGCGCY 2 cut(s) 147, 172
BfuAI ACCTGC 2 cut(s) 1256, 1458
BglI GCCNNNNNGGC 1 cut(s) 2249
BisI GCNGC 7 cut(s) 78, 192, 288, 324, 576, 1986, 2314
BlpI GCTNAGC 1 cut(s) 1453
BlsI GCNGC 7 cut(s) 79, 193, 289, 325, 577, 1987, 2315
Bme18I GGWCC 5 cut(s) 274, 467, 739, 1049, 1958
BmeT110I CYCGRG 3 cut(s) 582, 1643, 1961
BmgT120I GGNCC 5 cut(s) 274, 467, 739, 1049, 1958
BmiI GGNNCC 5 cut(s) 145, 207, 276, 1215, 1960
BmrI ACTGGG 1 cut(s) 866
BmtI GCTAGC 1 cut(s) 2348
BmuI ACTGGG 1 cut(s) 866
BoxI GACNNNNGTC 1 cut(s) 134
BpiI GAAGAC 3 cut(s) 738, 936, 2184
BpmI CTGGAG 7 cut(s) 348, 939, 1005, 1386, 1462, 1629, 1785
Bpu10I CCTNAGC 3 cut(s) 7, 248, 1800
Bpu1102I GCTNAGC 1 cut(s) 1453
Bpu14I TTCGAA 1 cut(s) 14
BpuMI CCSGG 5 cut(s) 47, 1644, 1645, 1932, 2043
Bsa29I ATCGAT 1 cut(s) 2110
BsaAI YACGTR 3 cut(s) 2027, 2072, 2164
BsaHI GRCGYC 4 cut(s) 26, 135, 144, 335
BsaWI WCCGGW 1 cut(s) 202
Bsc4I CCNNNNNNNGG 8 cut(s) 52, 153, 748, 877, 878, 1124, 1955, 2075
Bse118I RCCGGY 2 cut(s) 140, 2194
Bse1I ACTGG 8 cut(s) 136, 872, 1134, 1369, 1445, 1658, 1906, 2186
Bse21I CCTNAGG 1 cut(s) 339
Bse3DI GCAATG 3 cut(s) 192, 1792, 1839
BseAI TCCGGA 1 cut(s) 202
BseBI CCWGG 7 cut(s) 760, 795, 984, 1120, 1608, 1713, 1764
BseCI ATCGAT 1 cut(s) 2110
BseGI GGATG 6 cut(s) 265, 418, 655, 1552, 1750, 2263
BseLI CCNNNNNNNGG 8 cut(s) 52, 153, 748, 877, 878, 1124, 1955, 2075
BseMI GCAATG 3 cut(s) 192, 1792, 1839
BseMII CTCAG 6 cut(s) 21, 330, 825, 1600, 1791, 1828
BseNI ACTGG 8 cut(s) 136, 872, 1134, 1369, 1445, 1658, 1906, 2186
BseRI GAGGAG 2 cut(s) 1817, 2061
BseXI GCAGC 4 cut(s) 274, 335, 587, 1997
Bsh1236I CGCG 3 cut(s) 70, 72, 159
BshFI GGCC 9 cut(s) 5, 110, 194, 253, 1177, 1278, 1967, 2090, 2185
BshNI GGYRCC 2 cut(s) 143, 1213
BshVI ATCGAT 1 cut(s) 2110
BsiHKAI GWGCWC 3 cut(s) 505, 545, 1600
BsiHKCI CYCGRG 3 cut(s) 582, 1643, 1961
BsiSI CCGG 8 cut(s) 47, 141, 203, 1644, 1931, 2043, 2195, 2249
BslFI GGGAC 2 cut(s) 1140, 1944
BslI CCNNNNNNNGG 8 cut(s) 52, 153, 748, 877, 878, 1124, 1955, 2075
BsmAI GTCTC 2 cut(s) 530, 1799
BsmBI CGTCTC 2 cut(s) 530, 1799
BsmFI GGGAC 2 cut(s) 1140, 1944
BsnI GGCC 9 cut(s) 5, 110, 194, 253, 1177, 1278, 1967, 2090, 2185
BsoBI CYCGRG 3 cut(s) 582, 1643, 1961
Bsp119I TTCGAA 1 cut(s) 14
Bsp1286I GDGCHC 3 cut(s) 505, 545, 1600
Bsp13I TCCGGA 1 cut(s) 202
Bsp143I GATC 9 cut(s) 151, 163, 180, 961, 1006, 1225, 1234, 1411, 2111
Bsp1720I GCTNAGC 1 cut(s) 1453
Bsp19I CCATGG 1 cut(s) 645
BspACI CCGC 8 cut(s) 43, 68, 77, 114, 191, 225, 410, 2313
BspANI GGCC 9 cut(s) 5, 110, 194, 253, 1177, 1278, 1967, 2090, 2185
BspCNI CTCAG 6 cut(s) 20, 331, 826, 1599, 1792, 1827
BspDI ATCGAT 1 cut(s) 2110
BspEI TCCGGA 1 cut(s) 202
BspFNI CGCG 3 cut(s) 70, 72, 159
BspLI GGNNCC 5 cut(s) 145, 207, 276, 1215, 1960
BspMI ACCTGC 2 cut(s) 1256, 1458
BspOI GCTAGC 1 cut(s) 2348
BspPI GGATC 4 cut(s) 159, 956, 1220, 1229
BspQI GCTCTTC 1 cut(s) 1975
BspT104I TTCGAA 1 cut(s) 14
BspT107I GGYRCC 2 cut(s) 143, 1213
BsrDI GCAATG 3 cut(s) 192, 1792, 1839
BsrFI RCCGGY 2 cut(s) 140, 2194
BsrI ACTGG 8 cut(s) 136, 872, 1134, 1369, 1445, 1658, 1906, 2186
BssAI RCCGGY 2 cut(s) 140, 2194
BssMI GATC 9 cut(s) 151, 163, 180, 961, 1006, 1225, 1234, 1411, 2111
BssNI GRCGYC 4 cut(s) 26, 135, 144, 335
BssT1I CCWWGG 2 cut(s) 645, 1279
Bst2UI CCWGG 7 cut(s) 760, 795, 984, 1120, 1608, 1713, 1764
Bst6I CTCTTC 2 cut(s) 713, 1975
BstACI GRCGYC 4 cut(s) 26, 135, 144, 335
BstAPI GCANNNNNTGC 1 cut(s) 1796
BstBAI YACGTR 3 cut(s) 2027, 2072, 2164
BstBI TTCGAA 1 cut(s) 14
BstC8I GCNNGC 7 cut(s) 142, 501, 868, 1709, 1861, 2077, 2346
BstDEI CTNAG 8 cut(s) 7, 248, 339, 834, 1453, 1586, 1800, 1814
BstDSI CCRYGG 2 cut(s) 645, 2168
BstF5I GGATG 6 cut(s) 265, 418, 655, 1552, 1750, 2263
BstFNI CGCG 3 cut(s) 70, 72, 159
BstH2I RGCGCY 2 cut(s) 147, 172
BstHHI GCGC 7 cut(s) 72, 82, 146, 159, 171, 1467, 2205
BstKTI GATC 9 cut(s) 154, 166, 183, 964, 1009, 1228, 1237, 1414, 2114
BstMAI GTCTC 2 cut(s) 530, 1799
BstMBI GATC 9 cut(s) 151, 163, 180, 961, 1006, 1225, 1234, 1411, 2111
BstMWI GCNNNNNNNGC 9 cut(s) 141, 191, 329, 1262, 1796, 2191, 2200, 2249, 2350
BstNI CCWGG 7 cut(s) 760, 795, 984, 1120, 1608, 1713, 1764
BstNSI RCATGY 3 cut(s) 398, 575, 2277
BstPAI GACNNNNGTC 1 cut(s) 134
BstSFI CTRYAG 3 cut(s) 228, 826, 1179
BstUI CGCG 3 cut(s) 70, 72, 159
BstV1I GCAGC 4 cut(s) 274, 335, 587, 1997
BstV2I GAAGAC 3 cut(s) 738, 936, 2184
BstX2I RGATCY 1 cut(s) 1225
BstYI RGATCY 1 cut(s) 1225
Bsu15I ATCGAT 1 cut(s) 2110
Bsu36I CCTNAGG 1 cut(s) 339
BsuRI GGCC 9 cut(s) 5, 110, 194, 253, 1177, 1278, 1967, 2090, 2185
BsuTUI ATCGAT 1 cut(s) 2110
BtgI CCRYGG 2 cut(s) 645, 2168
BtgZI GCGATG 1 cut(s) 174
BtsCI GGATG 6 cut(s) 265, 418, 655, 1552, 1750, 2263
BtsI GCAGTG 2 cut(s) 312, 2259
BtsIMutI CAGTG 4 cut(s) 312, 1362, 1491, 2259
BveI ACCTGC 2 cut(s) 1256, 1458
Cac8I GCNNGC 7 cut(s) 142, 501, 868, 1709, 1861, 2077, 2346
CfoI GCGC 7 cut(s) 72, 82, 146, 159, 171, 1467, 2205
Cfr10I RCCGGY 2 cut(s) 140, 2194
Cfr13I GGNCC 5 cut(s) 274, 467, 739, 1049, 1958
Cfr9I CCCGGG 1 cut(s) 1643
ClaI ATCGAT 1 cut(s) 2110
CseI GACGC 4 cut(s) 34, 124, 343, 1816
Csp6I GTAC 7 cut(s) 418, 569, 1072, 1500, 1874, 1928, 2165
CviQI GTAC 7 cut(s) 418, 569, 1072, 1500, 1874, 1928, 2165
DdeI CTNAG 8 cut(s) 7, 248, 339, 834, 1453, 1586, 1800, 1814
DinI GGCGCC 1 cut(s) 145
DpnI GATC 9 cut(s) 153, 165, 182, 963, 1008, 1227, 1236, 1413, 2113
DpnII GATC 9 cut(s) 151, 163, 180, 961, 1006, 1225, 1234, 1411, 2111
DraIII CACNNNGTG 1 cut(s) 2270
DrdI GACNNNNNNGTC 1 cut(s) 2186
DseDI GACNNNNNNGTC 1 cut(s) 2186
EaeI YGGCCR 1 cut(s) 2088
Eam1104I CTCTTC 2 cut(s) 713, 1975
EarI CTCTTC 2 cut(s) 713, 1975
EciI GGCGGA 2 cut(s) 32, 103
Ecl136II GAGCTC 1 cut(s) 503
Eco130I CCWWGG 2 cut(s) 645, 1279
Eco147I AGGCCT 2 cut(s) 253, 1177
Eco24I GRGCYC 1 cut(s) 505
Eco47I GGWCC 5 cut(s) 274, 467, 739, 1049, 1958
Eco53kI GAGCTC 1 cut(s) 503
Eco57I CTGAAG 4 cut(s) 585, 1065, 1584, 2015
Eco72I CACGTG 2 cut(s) 2027, 2072
Eco81I CCTNAGG 1 cut(s) 339
Eco88I CYCGRG 3 cut(s) 582, 1643, 1961
EcoICRI GAGCTC 1 cut(s) 503
EcoRI GAATTC 1 cut(s) 1419
EcoRII CCWGG 7 cut(s) 758, 793, 982, 1118, 1606, 1711, 1762
EcoT14I CCWWGG 2 cut(s) 645, 1279
EcoT22I ATGCAT 1 cut(s) 1792
EcoT38I GRGCYC 1 cut(s) 505
EgeI GGCGCC 1 cut(s) 145
EheI GGCGCC 1 cut(s) 145
ErhI CCWWGG 2 cut(s) 645, 1279
Esp3I CGTCTC 2 cut(s) 530, 1799
FalI AAGNNNNNCTT 6 cut(s) 1263, 1295, 1557, 1589, 1972, 2004
FaqI GGGAC 2 cut(s) 1140, 1944
FauNDI CATATG 2 cut(s) 1150, 1219
FbaI TGATCA 1 cut(s) 163
Fnu4HI GCNGC 7 cut(s) 78, 192, 288, 324, 576, 1986, 2314
FokI GGATG 6 cut(s) 252, 425, 662, 1559, 1757, 2270
FriOI GRGCYC 1 cut(s) 505
Fsp4HI GCNGC 7 cut(s) 78, 192, 288, 324, 576, 1986, 2314
FspBI CTAG 2 cut(s) 380, 2345
FspI TGCGCA 1 cut(s) 1466
GlaI GCGC 7 cut(s) 71, 81, 145, 158, 170, 1466, 2204
GluI GCNGC 7 cut(s) 78, 192, 288, 324, 576, 1986, 2314
GsuI CTGGAG 7 cut(s) 348, 939, 1005, 1386, 1462, 1629, 1785
HaeII RGCGCY 2 cut(s) 147, 172
HaeIII GGCC 9 cut(s) 5, 110, 194, 253, 1177, 1278, 1967, 2090, 2185
HapII CCGG 8 cut(s) 47, 141, 203, 1644, 1931, 2043, 2195, 2249
HgaI GACGC 4 cut(s) 34, 124, 343, 1816
HhaI GCGC 7 cut(s) 72, 82, 146, 159, 171, 1467, 2205
Hin1I GRCGYC 4 cut(s) 26, 135, 144, 335
Hin6I GCGC 7 cut(s) 70, 80, 144, 157, 169, 1465, 2203
HinP1I GCGC 7 cut(s) 70, 80, 144, 157, 169, 1465, 2203
HincII GTYRAC 2 cut(s) 1156, 1911
HindII GTYRAC 2 cut(s) 1156, 1911
HindIII AAGCTT 2 cut(s) 938, 1054
HinfI GANTC 6 cut(s) 50, 199, 1110, 1823, 2154, 2232
HpaI GTTAAC 1 cut(s) 1911
HpaII CCGG 8 cut(s) 47, 141, 203, 1644, 1931, 2043, 2195, 2249
HphI GGTGA 3 cut(s) 928, 1280, 2023
Hpy166II GTNNAC 6 cut(s) 467, 517, 590, 1156, 1780, 1911
Hpy188I TCNGA 6 cut(s) 565, 619, 1828, 2153, 2334, 2357
Hpy188III TCNNGA 9 cut(s) 203, 629, 1436, 1473, 1544, 1816, 1961, 1993, 2117
Hpy8I GTNNAC 6 cut(s) 467, 517, 590, 1156, 1780, 1911
Hpy99I CGWCG 3 cut(s) 28, 140, 337
HpyAV CCTTC 8 cut(s) 39, 219, 1040, 1167, 1300, 1470, 2075, 2284
HpyCH4IV ACGT 3 cut(s) 2026, 2071, 2163
HpyF10VI GCNNNNNNNGC 9 cut(s) 141, 191, 329, 1262, 1796, 2191, 2200, 2249, 2350
HpyF3I CTNAG 8 cut(s) 7, 248, 339, 834, 1453, 1586, 1800, 1814
HpySE526I ACGT 3 cut(s) 2026, 2071, 2163
Hsp92I GRCGYC 4 cut(s) 26, 135, 144, 335
HspAI GCGC 7 cut(s) 70, 80, 144, 157, 169, 1465, 2203
KasI GGCGCC 1 cut(s) 143
Kpn2I TCCGGA 1 cut(s) 202
KroI GCCGGC 1 cut(s) 140
KroNI GCCGGC 1 cut(s) 142
Ksp22I TGATCA 1 cut(s) 163
KspAI GTTAAC 1 cut(s) 1911
Kzo9I GATC 9 cut(s) 151, 163, 180, 961, 1006, 1225, 1234, 1411, 2111
LguI GCTCTTC 1 cut(s) 1975
LmnI GCTCC 5 cut(s) 329, 492, 796, 1595, 2200
Lsp1109I GCAGC 4 cut(s) 274, 335, 587, 1997
MaeI CTAG 2 cut(s) 380, 2345
MaeII ACGT 3 cut(s) 2026, 2071, 2163
MaeIII GTNAC 4 cut(s) 839, 860, 1838, 2011
MalI GATC 9 cut(s) 153, 165, 182, 963, 1008, 1227, 1236, 1413, 2113
MboI GATC 9 cut(s) 151, 163, 180, 961, 1006, 1225, 1234, 1411, 2111
MflI RGATCY 1 cut(s) 1225
MhlI GDGCHC 3 cut(s) 505, 545, 1600
MlsI TGGCCA 1 cut(s) 2090
MluCI AATT 6 cut(s) 1032, 1096, 1387, 1419, 1510, 1554
MluNI TGGCCA 1 cut(s) 2090
Mly113I GGCGCC 1 cut(s) 144
MmeI TCCRAC 4 cut(s) 117, 460, 2154, 2262
Mox20I TGGCCA 1 cut(s) 2090
Mph1103I ATGCAT 1 cut(s) 1792
MreI CGCCGGCG 1 cut(s) 140
MroI TCCGGA 1 cut(s) 202
MroNI GCCGGC 1 cut(s) 140
MroXI GAANNNNTTC 3 cut(s) 372, 624, 2037
MscI TGGCCA 1 cut(s) 2090
MseI TTAA 7 cut(s) 1065, 1328, 1569, 1749, 1893, 1910, 2100
MslI CAYNNNNRTG 4 cut(s) 914, 1495, 1785, 2195
Msp20I TGGCCA 1 cut(s) 2090
MspA1I CMGCKG 3 cut(s) 326, 959, 2313
MspI CCGG 8 cut(s) 47, 141, 203, 1644, 1931, 2043, 2195, 2249
MvaI CCWGG 7 cut(s) 760, 795, 984, 1120, 1608, 1713, 1764
MvnI CGCG 3 cut(s) 70, 72, 159
MwoI GCNNNNNNNGC 9 cut(s) 141, 191, 329, 1262, 1796, 2191, 2200, 2249, 2350
NaeI GCCGGC 1 cut(s) 142
NarI GGCGCC 1 cut(s) 144
NciI CCSGG 5 cut(s) 47, 1644, 1645, 1932, 2043
NcoI CCATGG 1 cut(s) 645
NdeI CATATG 2 cut(s) 1150, 1219
NdeII GATC 9 cut(s) 151, 163, 180, 961, 1006, 1225, 1234, 1411, 2111
NgoMIV GCCGGC 1 cut(s) 140
NheI GCTAGC 1 cut(s) 2344
NlaIV GGNNCC 5 cut(s) 145, 207, 276, 1215, 1960
NmeAIII GCCGAG 2 cut(s) 172, 475
NmuCI GTSAC 3 cut(s) 839, 1838, 2011
NsbI TGCGCA 1 cut(s) 1466
NsiI ATGCAT 1 cut(s) 1792
NspI RCATGY 3 cut(s) 398, 575, 2277
NspV TTCGAA 1 cut(s) 14
PaeR7I CTCGAG 1 cut(s) 582
PaqCI CACCTGC 1 cut(s) 1256
PasI CCCWGGG 1 cut(s) 1119
PceI AGGCCT 2 cut(s) 253, 1177
PciI ACATGT 1 cut(s) 394
PciSI GCTCTTC 1 cut(s) 1975
PcsI WCGNNNNNNNCGW 5 cut(s) 20, 69, 144, 502, 1882
PdiI GCCGGC 1 cut(s) 142
PdmI GAANNNNTTC 3 cut(s) 372, 624, 2037
PfeI GAWTC 6 cut(s) 50, 199, 1110, 1823, 2154, 2232
PflMI CCANNNNNTGG 2 cut(s) 1955, 2075
PfoI TCCNGGA 2 cut(s) 982, 2041
PkrI GCNGC 7 cut(s) 79, 193, 289, 325, 577, 1987, 2315
PluTI GGCGCC 1 cut(s) 147
PmaCI CACGTG 2 cut(s) 2027, 2072
PmlI CACGTG 2 cut(s) 2027, 2072
Ppu21I YACGTR 3 cut(s) 2027, 2072, 2164
PscI ACATGT 1 cut(s) 394
PshAI GACNNNNGTC 1 cut(s) 134
Psp124BI GAGCTC 1 cut(s) 505
Psp6I CCWGG 7 cut(s) 758, 793, 982, 1118, 1606, 1711, 1762
PspCI CACGTG 2 cut(s) 2027, 2072
PspGI CCWGG 7 cut(s) 758, 793, 982, 1118, 1606, 1711, 1762
PspN4I GGNNCC 5 cut(s) 145, 207, 276, 1215, 1960
PspPI GGNCC 5 cut(s) 274, 467, 739, 1049, 1958
PspXI VCTCGAGB 1 cut(s) 582
PsuI RGATCY 1 cut(s) 1225
PvuII CAGCTG 2 cut(s) 326, 959
RsaI GTAC 7 cut(s) 419, 570, 1073, 1501, 1875, 1929, 2166
RsaNI GTAC 7 cut(s) 418, 569, 1072, 1500, 1874, 1928, 2165
RseI CAYNNNNRTG 4 cut(s) 914, 1495, 1785, 2195
SacI GAGCTC 1 cut(s) 505
SapI GCTCTTC 1 cut(s) 1975
SaqAI TTAA 7 cut(s) 1065, 1328, 1569, 1749, 1893, 1910, 2100
SatI GCNGC 7 cut(s) 78, 192, 288, 324, 576, 1986, 2314
Sau3AI GATC 9 cut(s) 151, 163, 180, 961, 1006, 1225, 1234, 1411, 2111
Sau96I GGNCC 5 cut(s) 274, 467, 739, 1049, 1958
SduI GDGCHC 3 cut(s) 505, 545, 1600
SfcI CTRYAG 3 cut(s) 228, 826, 1179
SfoI GGCGCC 1 cut(s) 145
Sfr274I CTCGAG 1 cut(s) 582
SfuI TTCGAA 1 cut(s) 14
SgrAI CRCCGGYG 1 cut(s) 140
SinI GGWCC 5 cut(s) 274, 467, 739, 1049, 1958
SlaI CTCGAG 1 cut(s) 582
SmaI CCCGGG 1 cut(s) 1645
SmiMI CAYNNNNRTG 4 cut(s) 914, 1495, 1785, 2195
SmlI CTYRAG 1 cut(s) 582
SmoI CTYRAG 1 cut(s) 582
Sse9I AATT 6 cut(s) 1032, 1096, 1387, 1419, 1510, 1554
SseBI AGGCCT 2 cut(s) 253, 1177
SsiI CCGC 8 cut(s) 43, 68, 77, 114, 191, 225, 410, 2313
SspDI GGCGCC 1 cut(s) 143
SspI AATATT 1 cut(s) 2021
SspMI CTAG 2 cut(s) 380, 2345
SstI GAGCTC 1 cut(s) 505
StuI AGGCCT 2 cut(s) 253, 1177
StyI CCWWGG 2 cut(s) 645, 1279
TaiI ACGT 3 cut(s) 2029, 2074, 2166
TaqI TCGA 7 cut(s) 14, 505, 583, 1009, 1246, 1383, 2110
TasI AATT 6 cut(s) 1032, 1096, 1387, 1419, 1510, 1554
TatI WGTACW 2 cut(s) 568, 1499
TauI GCSGC 3 cut(s) 80, 194, 2316
TfiI GAWTC 6 cut(s) 50, 199, 1110, 1823, 2154, 2232
Tru1I TTAA 7 cut(s) 1065, 1328, 1569, 1749, 1893, 1910, 2100
Tru9I TTAA 7 cut(s) 1065, 1328, 1569, 1749, 1893, 1910, 2100
TscAI CASTG 4 cut(s) 312, 1369, 1491, 2259
TseFI GTSAC 3 cut(s) 839, 1838, 2011
TseI GCWGC 4 cut(s) 287, 323, 575, 1985
Tsp45I GTSAC 3 cut(s) 839, 1838, 2011
TspDTI ATGAA 2 cut(s) 728, 2046
TspGWI ACGGA 2 cut(s) 1891, 2244
TspMI CCCGGG 1 cut(s) 1643
TspRI CASTG 4 cut(s) 312, 1369, 1491, 2259
Van91I CCANNNNNTGG 2 cut(s) 1955, 2075
VpaK11BI GGWCC 5 cut(s) 274, 467, 739, 1049, 1958
XapI RAATTY 2 cut(s) 1096, 1419
XceI RCATGY 3 cut(s) 398, 575, 2277
XcmI CCANNNNNNNNNTGG 2 cut(s) 652, 1126
XhoI CTCGAG 1 cut(s) 582
XmaI CCCGGG 1 cut(s) 1643
XmnI GAANNNNTTC 3 cut(s) 372, 624, 2037
XspI CTAG 2 cut(s) 380, 2345
Zsp2I ATGCAT 1 cut(s) 1792
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.