pycom14g14630

Galactinol--sucrose galactosyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0005392
Physical Location & Seq
Reverse (-)
17365764 .. 17370512
4749 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2325 bp
ATGGCTTCAAGCATAACTAAGATTAATGTAGCCCTTGTGAATGACACGTCACCACTCCCACTAACTCTCAAAGGATCCAACCTTCTCGCTAATGGCCACCCAATCCTCACTGAAGTCCCTCTCAACATCACAGCTACCCTGTCACCTTTTAACAAGACCCCCTCCGGCTGCTTCCTTGGCTTCGACGCCGACGAGTCTAGGAGCCGCCACGTGGTCCATATCGGAAAACTCACTTCGATCAAGTTCATGAGCATATTCCGGTTCAAGGTCTGGTGGGCCACCCGCTGGACCGGAACCACCGGAAACGACTTGGAGCACGAGACCCAGATGATGCTCTTGGACAAAAACGACTCCGGGCTCCCTTACGTTGTGATTCTTCCACTTCTGGAAGGACCATTCAGAGCCTCTCTCCAACCGAGTCATGCAAACGATGACTACGTGGACATTTGCATGGAAAGCGGCTCGACACGTGTCAGCGCATCGAACTTCAGGAGCTGCTTGTATATGCAAGGTAGCGGTGATGACCCGTATAGCTTGGTCAAGGAGGCAATGAAGGTGGTGAGGGCCCACTTGGGGACGTTCAAGCTCCTAGAGGAAAAAACCGTACCAAAAATAGTGGACAAGTTTGGGTGGTGTACGTGGGATGCATTCTATCTTAATGTGCACCCCAGAGGGGTGTGGGAAGGTGTGAAGGGCCTGGTGGAGGGTGGGTGCCCTCCAGGACTGGTCCTCATTGATGATGGGTGGCAATCAATCTGTCCCGACGAGGACTCAGTGGAGGGCCAAGAAGGCATGGCCAGGATATCAGCTGGGGAACAAATGCCATGCAGGCTGATTAGTTTTAAAGAGAATTATAAATTTAAGGAGTACGAGGGGGCGTCTGGCAAGGGCTTGGGTGGCTTTGTTAAGGATTTGAAGGAGGAATTTGGGAGTGTGGAGCATGTGTATGTGTGGCATGCGCTTTGTGGCTATTGGGGTGGGATTAAGCCTGGGATTAATAGTCCAGGGATGCCCGAAGAGTGCTGCAGGGTGGTTAGTCCTAAGTTGTCTCCGGGATTAGAGAGGACAATGGAGGACCTGGCGGTGGACAAGATTGTGAGGAATGGAGTTGGGTTGGTTCTGCCGGAGGTGGCCCACAAGCTATATGAGGGGATTCACTCTCATCTTCAGTCGGTGGGGATTGATGGTGTCAAGGTTGATGTCATAAACTTGCTTGAGATGTTATCCGAGGATTTTGGCGGTCAAGTTGAGTTGGCTAAAGCATATTACAAGGCACTGACTGCTTCTGTGAGGAAGCATTTCAATGGCAATGGAGTGATTGCTAGCATGCAGCACAGCAATTACTTTATGTACCTCGGAACCGAGGCCATAGCCCTTGGACGTGTTGGAGATGATTTCTGTTCTGAGACGATGGAGCAAGCAGGTGGGACATATTGGTTGCAAGGGTGTCACGTGGTGCACTGCGCCTACAATAGCCTTTGGATAGGCAACATCATACAGCCAGATTGGGACATGTTTCAATCTACGCACCCGTGCGCTGAGTTCCATGCTGCATCAAGAGCCATATCTGGAGGACCAATCTACATTAGTGACTCTGTTGGAAAGCACAACTTCAAGCTTCTTAAGAACCTGGTGTTGCCAGATGGGTCCGTACTGCGATGCCAACACTATGCCCTTCCTTCTAGGGATTGCCTATTTGAAGACCCTGTGCATGATGGGAAAACAATGCTCAAGATATGGAACCTCAACAAATACACTGGAGTTTTAGGTGTTTTTAATTGTCAAGGCGGAGGATGGTGCTCTAAATCCAGGAGTAACAAAATTGCCCCCGACTGCTCAAAGTCTGTGACCTGTTTGGCTAGCCCAAAAGATGTCGAATGGAATCAAGGGAAGAATCCAATTTCAATCAAAGGAGTGAACACATTTGCTGTGTATATGTTCCAGCAAAACAAGCTGAAGCTCTTGCAGTCATCAGAGAAGATAGAGATTTCACTTGAGCCTTTTACTTTTGAGCTCCTCACCGTTTCGCCGGTGAGGGTCTTGCCGAAGAAACCGATCCAATTTGCTCCGATTGGATTGGTCAACATGCTTAACACTGGCGGTGCAATTGAGTCAGTAGAATTTGAGGAACATGAAGATTCCCTTAGTTTGGTGAGAATTGGAATGAAGGGTTGTGGAGAAATGAGAATGTTTGCTTCAGAAAAGCCAATTGCTTGTATGATCGATGGGGAGGGAGTGAAGTTTCATTACGTTGATAAGATGGTCAAAGTCCAGGTGCCATGGCTTAGTTCTTCAAGGTCAACTTTAGTTGAGTACCTGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

775

Amino Acids

85.52

Weight (kDa)

6.05

Isoelectric Point (pI)

41.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 25 - 757 0.0 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 855
AarI CACCTGC 1 cut(s) 1412
Acc36I ACCTGC 1 cut(s) 1412
AccB1I GGYRCC 2 cut(s) 711, 2275
AccB7I CCANNNNNTGG 1 cut(s) 285
AciI CCGC 8 cut(s) 205, 283, 459, 516, 1082, 1239, 1788, 2100
AclWI GGATC 3 cut(s) 69, 82, 2050
AcoI YGGCCR 2 cut(s) 94, 795
AcsI RAATTY 3 cut(s) 857, 923, 2120
AcuI CTGAAG 5 cut(s) 132, 472, 1151, 1976, 2181
AcvI CACGTG 3 cut(s) 211, 470, 1453
AcyI GRCGYC 2 cut(s) 186, 878
AdeI CACNNNGTG 1 cut(s) 1456
AfaI GTAC 6 cut(s) 606, 637, 869, 1352, 1653, 2315
AfiI CCNNNNNNNGG 8 cut(s) 211, 265, 285, 573, 673, 703, 1084, 2149
AflII CTTAAG 1 cut(s) 1622
AflIII ACRYGT 5 cut(s) 45, 467, 469, 1381, 1512
AjiI CACGTC 2 cut(s) 48, 1382
AjnI CCWGG 9 cut(s) 696, 718, 797, 988, 1003, 1077, 1629, 1808, 2271
Alw21I GWGCWC 5 cut(s) 318, 666, 1461, 1802, 2016
Alw26I GTCTC 3 cut(s) 314, 1053, 1400
Alw44I GTGCAC 2 cut(s) 662, 1457
AlwI GGATC 3 cut(s) 69, 82, 2050
AlwNI CAGNNNCTG 1 cut(s) 495
AoxI GGCC 8 cut(s) 94, 276, 564, 694, 781, 795, 1131, 1365
ApaI GGGCCC 1 cut(s) 568
ApaLI GTGCAC 2 cut(s) 662, 1457
ApeKI GCWGC 5 cut(s) 168, 495, 1023, 1330, 1550
ApoI RAATTY 3 cut(s) 857, 923, 2120
AseI ATTAAT 2 cut(s) 24, 996
Asp700I GAANNNNTTC 1 cut(s) 1298
AspLEI GCGC 4 cut(s) 479, 961, 1466, 1538
AsuC2I CCSGG 2 cut(s) 355, 1053
AsuHPI GGTGA 7 cut(s) 42, 135, 530, 571, 2011, 2044, 2164
AsuNHI GCTAGC 2 cut(s) 1322, 1859
AvaII GGWCC 7 cut(s) 214, 288, 392, 727, 1075, 1574, 1647
BaeGI GKGCMC 4 cut(s) 568, 666, 716, 1461
BalI TGGCCA 2 cut(s) 96, 797
BamHI GGATCC 1 cut(s) 74
BanI GGYRCC 2 cut(s) 711, 2275
BanII GRGCYC 3 cut(s) 360, 568, 2016
BarI GAAGNNNNNNTAC 2 cut(s) 2231, 2263
BauI CACGAG 1 cut(s) 317
BbrPI CACGTG 3 cut(s) 211, 470, 1453
BbsI GAAGAC 1 cut(s) 1707
Bbv12I GWGCWC 5 cut(s) 318, 666, 1461, 1802, 2016
BbvI GCAGC 5 cut(s) 155, 482, 1010, 1342, 1537
BccI CCATC 8 cut(s) 734, 1178, 1405, 1637, 1709, 1788, 2219, 2254
BciT130I CCWGG 9 cut(s) 698, 720, 799, 990, 1005, 1079, 1631, 1810, 2273
BcnI CCSGG 2 cut(s) 355, 1053
BcoDI GTCTC 3 cut(s) 314, 1053, 1400
BfaI CTAG 5 cut(s) 198, 590, 1323, 1683, 1860
BfmI CTRYAG 1 cut(s) 1024
BfrI CTTAAG 1 cut(s) 1622
BfuAI ACCTGC 1 cut(s) 1412
BglI GCCNNNNNGGC 2 cut(s) 789, 829
BisI GCNGC 7 cut(s) 169, 205, 460, 496, 1024, 1331, 1551
BlsI GCNGC 7 cut(s) 170, 206, 461, 497, 1025, 1332, 1552
Bme18I GGWCC 7 cut(s) 214, 288, 392, 727, 1075, 1574, 1647
BmgBI CACGTC 2 cut(s) 48, 1382
BmsI GCATC 6 cut(s) 321, 488, 634, 999, 1562, 1649
BmtI GCTAGC 2 cut(s) 1326, 1863
BoxI GACNNNNGTC 1 cut(s) 470
BpiI GAAGAC 1 cut(s) 1707
BplI GAGNNNNNCTC 2 cut(s) 393, 425
BpmI CTGGAG 3 cut(s) 702, 1590, 1779
BpuEI CTTGAG 3 cut(s) 1235, 1715, 2015
BpuMI CCSGG 2 cut(s) 355, 1053
Bsa29I ATCGAT 1 cut(s) 2223
BsaAI YACGTR 5 cut(s) 211, 439, 470, 639, 1453
BsaHI GRCGYC 2 cut(s) 186, 878
BsaI GGTCTC 1 cut(s) 314
BsaJI CCNNGG 8 cut(s) 175, 989, 1004, 1227, 1354, 1362, 1375, 2279
BsaWI WCCGGW 3 cut(s) 258, 290, 299
BsaXI ACNNNNNCTCC 4 cut(s) 695, 725, 929, 959
Bsc4I CCNNNNNNNGG 8 cut(s) 211, 265, 285, 573, 673, 703, 1084, 2149
Bse118I RCCGGY 1 cut(s) 2029
Bse1I ACTGG 3 cut(s) 729, 1762, 2101
Bse3DI GCAATG 2 cut(s) 555, 1315
BseBI CCWGG 9 cut(s) 698, 720, 799, 990, 1005, 1079, 1631, 1810, 2273
BseCI ATCGAT 1 cut(s) 2223
BseDI CCNNGG 8 cut(s) 175, 989, 1004, 1227, 1354, 1362, 1375, 2279
BseGI GGATG 3 cut(s) 649, 1014, 1799
BseLI CCNNNNNNNGG 8 cut(s) 211, 265, 285, 573, 673, 703, 1084, 2149
BseMI GCAATG 2 cut(s) 555, 1315
BseMII CTCAG 3 cut(s) 786, 1395, 1530
BseNI ACTGG 3 cut(s) 729, 1762, 2101
BseRI GAGGAG 1 cut(s) 2006
BseSI GKGCMC 4 cut(s) 568, 666, 716, 1461
BseXI GCAGC 5 cut(s) 155, 482, 1010, 1342, 1537
BseYI CCCAGC 1 cut(s) 809
BshFI GGCC 8 cut(s) 96, 278, 566, 696, 783, 797, 1133, 1367
BshNI GGYRCC 2 cut(s) 711, 2275
BshVI ATCGAT 1 cut(s) 2223
BsiHKAI GWGCWC 5 cut(s) 318, 666, 1461, 1802, 2016
BsiSI CCGG 8 cut(s) 165, 259, 291, 300, 354, 1052, 1124, 2030
BslFI GGGAC 5 cut(s) 101, 589, 744, 1441, 1523
BslI CCNNNNNNNGG 8 cut(s) 211, 265, 285, 573, 673, 703, 1084, 2149
BsmAI GTCTC 3 cut(s) 314, 1053, 1400
BsmBI CGTCTC 1 cut(s) 1400
BsmFI GGGAC 5 cut(s) 101, 589, 744, 1441, 1523
BsmI GAATGC 1 cut(s) 647
BsnI GGCC 8 cut(s) 96, 278, 566, 696, 783, 797, 1133, 1367
Bso31I GGTCTC 1 cut(s) 314
Bsp120I GGGCCC 1 cut(s) 564
Bsp1286I GDGCHC 8 cut(s) 318, 360, 568, 666, 716, 1461, 1802, 2016
Bsp143I GATC 4 cut(s) 74, 237, 2055, 2220
Bsp19I CCATGG 1 cut(s) 2279
BspACI CCGC 8 cut(s) 205, 283, 459, 516, 1082, 1239, 1788, 2100
BspANI GGCC 8 cut(s) 96, 278, 566, 696, 783, 797, 1133, 1367
BspCNI CTCAG 3 cut(s) 785, 1396, 1531
BspDI ATCGAT 1 cut(s) 2223
BspHI TCATGA 1 cut(s) 246
BspMAI CTGCAG 1 cut(s) 1028
BspMI ACCTGC 1 cut(s) 1412
BspOI GCTAGC 2 cut(s) 1326, 1863
BspPI GGATC 3 cut(s) 69, 82, 2050
BspT107I GGYRCC 2 cut(s) 711, 2275
BspTI CTTAAG 1 cut(s) 1622
BspTNI GGTCTC 1 cut(s) 314
BsrDI GCAATG 2 cut(s) 555, 1315
BsrFI RCCGGY 1 cut(s) 2029
BsrI ACTGG 3 cut(s) 729, 1762, 2101
BssAI RCCGGY 1 cut(s) 2029
BssECI CCNNGG 8 cut(s) 175, 989, 1004, 1227, 1354, 1362, 1375, 2279
BssMI GATC 4 cut(s) 74, 237, 2055, 2220
BssNI GRCGYC 2 cut(s) 186, 878
BssSI CACGAG 1 cut(s) 317
BssT1I CCWWGG 3 cut(s) 175, 1375, 2279
Bst2BI CACGAG 1 cut(s) 317
Bst2UI CCWGG 9 cut(s) 698, 720, 799, 990, 1005, 1079, 1631, 1810, 2273
Bst4CI ACNGT 2 cut(s) 604, 2023
Bst6I CTCTTC 1 cut(s) 1011
BstACI GRCGYC 2 cut(s) 186, 878
BstAFI CTTAAG 1 cut(s) 1622
BstAPI GCANNNNNTGC 1 cut(s) 1280
BstBAI YACGTR 5 cut(s) 211, 439, 470, 639, 1453
BstC8I GCNNGC 6 cut(s) 830, 957, 1324, 1328, 1419, 1861
BstDEI CTNAG 7 cut(s) 18, 772, 1041, 1404, 1539, 2144, 2285
BstDSI CCRYGG 1 cut(s) 2279
BstENI CCTNNNNNAGG 1 cut(s) 701
BstF5I GGATG 3 cut(s) 649, 1014, 1799
BstHHI GCGC 4 cut(s) 479, 961, 1466, 1538
BstKTI GATC 4 cut(s) 77, 240, 2058, 2223
BstMAI GTCTC 3 cut(s) 314, 1053, 1400
BstMBI GATC 4 cut(s) 74, 237, 2055, 2220
BstMWI GCNNNNNNNGC 8 cut(s) 177, 456, 789, 829, 897, 1280, 1559, 1951
BstNI CCWGG 9 cut(s) 698, 720, 799, 990, 1005, 1079, 1631, 1810, 2273
BstNSI RCATGY 5 cut(s) 944, 959, 1330, 1516, 2089
BstPAI GACNNNNGTC 1 cut(s) 470
BstSFI CTRYAG 1 cut(s) 1024
BstSLI GKGCMC 4 cut(s) 568, 666, 716, 1461
BstV1I GCAGC 5 cut(s) 155, 482, 1010, 1342, 1537
BstV2I GAAGAC 1 cut(s) 1707
BstX2I RGATCY 1 cut(s) 74
BstYI RGATCY 1 cut(s) 74
Bsu15I ATCGAT 1 cut(s) 2223
BsuRI GGCC 8 cut(s) 96, 278, 566, 696, 783, 797, 1133, 1367
BsuTUI ATCGAT 1 cut(s) 2223
BtgI CCRYGG 1 cut(s) 2279
BtgZI GCGATG 1 cut(s) 1672
BtrI CACGTC 2 cut(s) 48, 1382
BtsCI GGATG 3 cut(s) 649, 1014, 1799
BtsI GCAGTG 1 cut(s) 1459
BtsIMutI CAGTG 6 cut(s) 108, 780, 1274, 1459, 1755, 2094
BveI ACCTGC 1 cut(s) 1412
Cac8I GCNNGC 6 cut(s) 830, 957, 1324, 1328, 1419, 1861
CaiI CAGNNNCTG 1 cut(s) 495
CciI TCATGA 1 cut(s) 246
CfoI GCGC 4 cut(s) 479, 961, 1466, 1538
Cfr10I RCCGGY 1 cut(s) 2029
ClaI ATCGAT 1 cut(s) 2223
CseI GACGC 2 cut(s) 194, 867
CsiI ACCWGGT 1 cut(s) 1629
Csp6I GTAC 6 cut(s) 605, 636, 868, 1351, 1652, 2314
CspCI CAANNNNNGTGG 4 cut(s) 537, 572, 597, 632
CviQI GTAC 6 cut(s) 605, 636, 868, 1351, 1652, 2314
DdeI CTNAG 7 cut(s) 18, 772, 1041, 1404, 1539, 2144, 2285
DpnI GATC 4 cut(s) 76, 239, 2057, 2222
DpnII GATC 4 cut(s) 74, 237, 2055, 2220
DraI TTTAAA 1 cut(s) 844
DraIII CACNNNGTG 1 cut(s) 1456
EaeI YGGCCR 2 cut(s) 94, 795
Eam1104I CTCTTC 1 cut(s) 1011
EarI CTCTTC 1 cut(s) 1011
EciI GGCGGA 1 cut(s) 1803
Ecl136II GAGCTC 1 cut(s) 2014
Eco130I CCWWGG 3 cut(s) 175, 1375, 2279
Eco24I GRGCYC 3 cut(s) 360, 568, 2016
Eco31I GGTCTC 1 cut(s) 314
Eco32I GATATC 1 cut(s) 804
Eco47I GGWCC 7 cut(s) 214, 288, 392, 727, 1075, 1574, 1647
Eco53kI GAGCTC 1 cut(s) 2014
Eco57I CTGAAG 5 cut(s) 132, 472, 1151, 1976, 2181
Eco72I CACGTG 3 cut(s) 211, 470, 1453
EcoICRI GAGCTC 1 cut(s) 2014
EcoNI CCTNNNNNAGG 1 cut(s) 701
EcoO109I RGGNCCY 3 cut(s) 564, 694, 1075
EcoRII CCWGG 9 cut(s) 696, 718, 797, 988, 1003, 1077, 1629, 1808, 2271
EcoRV GATATC 1 cut(s) 804
EcoT14I CCWWGG 3 cut(s) 175, 1375, 2279
EcoT22I ATGCAT 1 cut(s) 649
EcoT38I GRGCYC 3 cut(s) 360, 568, 2016
ErhI CCWWGG 3 cut(s) 175, 1375, 2279
Esp3I CGTCTC 1 cut(s) 1400
FalI AAGNNNNNCTT 6 cut(s) 1595, 1627, 2127, 2159, 2287, 2319
FaqI GGGAC 5 cut(s) 101, 589, 744, 1441, 1523
FauI CCCGC 1 cut(s) 290
Fnu4HI GCNGC 7 cut(s) 169, 205, 460, 496, 1024, 1331, 1551
FokI GGATG 3 cut(s) 656, 1021, 1806
FriOI GRGCYC 3 cut(s) 360, 568, 2016
Fsp4HI GCNGC 7 cut(s) 169, 205, 460, 496, 1024, 1331, 1551
FspBI CTAG 5 cut(s) 198, 590, 1323, 1683, 1860
GlaI GCGC 4 cut(s) 478, 960, 1465, 1537
GluI GCNGC 7 cut(s) 169, 205, 460, 496, 1024, 1331, 1551
GsaI CCCAGC 1 cut(s) 813
GsuI CTGGAG 3 cut(s) 702, 1590, 1779
HaeIII GGCC 8 cut(s) 96, 278, 566, 696, 783, 797, 1133, 1367
HapII CCGG 8 cut(s) 165, 259, 291, 300, 354, 1052, 1124, 2030
HgaI GACGC 2 cut(s) 194, 867
HhaI GCGC 4 cut(s) 479, 961, 1466, 1538
Hin1I GRCGYC 2 cut(s) 186, 878
Hin6I GCGC 4 cut(s) 477, 959, 1464, 1536
HinP1I GCGC 4 cut(s) 477, 959, 1464, 1536
HincII GTYRAC 2 cut(s) 2083, 2301
HindII GTYRAC 2 cut(s) 2083, 2301
HindIII AAGCTT 1 cut(s) 1616
HpaII CCGG 8 cut(s) 165, 259, 291, 300, 354, 1052, 1124, 2030
HphI GGTGA 7 cut(s) 42, 135, 530, 571, 2011, 2044, 2164
Hpy166II GTNNAC 9 cut(s) 442, 619, 636, 664, 1087, 1459, 1918, 2083, 2301
Hpy188I TCNGA 8 cut(s) 224, 401, 1228, 1358, 1405, 1975, 2070, 2200
Hpy188III TCNNGA 7 cut(s) 247, 386, 490, 761, 1557, 1569, 1732
Hpy8I GTNNAC 9 cut(s) 442, 619, 636, 664, 1087, 1459, 1918, 2083, 2301
Hpy99I CGWCG 3 cut(s) 188, 194, 767
HpyCH4III ACNGT 2 cut(s) 604, 2023
HpyF10VI GCNNNNNNNGC 8 cut(s) 177, 456, 789, 829, 897, 1280, 1559, 1951
HpyF3I CTNAG 7 cut(s) 18, 772, 1041, 1404, 1539, 2144, 2285
Hsp92I GRCGYC 2 cut(s) 186, 878
HspAI GCGC 4 cut(s) 477, 959, 1464, 1536
Kzo9I GATC 4 cut(s) 74, 237, 2055, 2220
LmnI GCTCC 9 cut(s) 201, 313, 363, 492, 591, 937, 1414, 2019, 2071
Lsp1109I GCAGC 5 cut(s) 155, 482, 1010, 1342, 1537
LweI GCATC 6 cut(s) 321, 488, 634, 999, 1562, 1649
MabI ACCWGGT 1 cut(s) 1629
MaeI CTAG 5 cut(s) 198, 590, 1323, 1683, 1860
MaeIII GTNAC 6 cut(s) 48, 141, 1448, 1589, 1814, 1846
MalI GATC 4 cut(s) 76, 239, 2057, 2222
MboI GATC 4 cut(s) 74, 237, 2055, 2220
MboII GAAGA 9 cut(s) 368, 1028, 1157, 1712, 1903, 1990, 2059, 2147, 2283
MfeI CAATTG 2 cut(s) 2106, 2208
MflI RGATCY 1 cut(s) 74
MhlI GDGCHC 8 cut(s) 318, 360, 568, 666, 716, 1461, 1802, 2016
MlsI TGGCCA 2 cut(s) 96, 797
MluNI TGGCCA 2 cut(s) 96, 797
MlyI GAGTC 6 cut(s) 203, 344, 427, 764, 1586, 2120
MmeI TCCRAC 4 cut(s) 102, 436, 1366, 1579
Mox20I TGGCCA 2 cut(s) 96, 797
Mph1103I ATGCAT 1 cut(s) 649
MroXI GAANNNNTTC 1 cut(s) 1298
MscI TGGCCA 2 cut(s) 96, 797
MslI CAYNNNNRTG 3 cut(s) 449, 945, 1302
Msp20I TGGCCA 2 cut(s) 96, 797
MspA1I CMGCKG 2 cut(s) 285, 809
MspCI CTTAAG 1 cut(s) 1622
MspI CCGG 8 cut(s) 165, 259, 291, 300, 354, 1052, 1124, 2030
MunI CAATTG 2 cut(s) 2106, 2208
Mva1269I GAATGC 1 cut(s) 647
MvaI CCWGG 9 cut(s) 698, 720, 799, 990, 1005, 1079, 1631, 1810, 2273
MwoI GCNNNNNNNGC 8 cut(s) 177, 456, 789, 829, 897, 1280, 1559, 1951
NciI CCSGG 2 cut(s) 355, 1053
NcoI CCATGG 1 cut(s) 2279
NdeII GATC 4 cut(s) 74, 237, 2055, 2220
NheI GCTAGC 2 cut(s) 1322, 1859
NmuCI GTSAC 5 cut(s) 48, 141, 1448, 1589, 1846
NsiI ATGCAT 1 cut(s) 649
NspI RCATGY 5 cut(s) 944, 959, 1330, 1516, 2089
PaeI GCATGC 2 cut(s) 959, 1330
PagI TCATGA 1 cut(s) 246
PaqCI CACCTGC 1 cut(s) 1412
PciI ACATGT 1 cut(s) 1512
PcsI WCGNNNNNNNCGW 2 cut(s) 189, 435
PctI GAATGC 1 cut(s) 647
PdmI GAANNNNTTC 1 cut(s) 1298
PfeI GAWTC 5 cut(s) 373, 1153, 1882, 1894, 2138
PflMI CCANNNNNTGG 1 cut(s) 285
PfoI TCCNGGA 3 cut(s) 718, 1051, 1808
PkrI GCNGC 7 cut(s) 170, 206, 461, 497, 1025, 1332, 1552
PleI GAGTC 6 cut(s) 202, 344, 426, 764, 1586, 2119
PmaCI CACGTG 3 cut(s) 211, 470, 1453
PmlI CACGTG 3 cut(s) 211, 470, 1453
PpsI GAGTC 6 cut(s) 202, 344, 426, 764, 1586, 2119
Ppu21I YACGTR 5 cut(s) 211, 439, 470, 639, 1453
PpuMI RGGWCCY 1 cut(s) 1075
PscI ACATGT 1 cut(s) 1512
PshAI GACNNNNGTC 1 cut(s) 470
PshBI ATTAAT 2 cut(s) 24, 996
PsiI TTATAA 1 cut(s) 855
Psp124BI GAGCTC 1 cut(s) 2016
Psp5II RGGWCCY 1 cut(s) 1075
Psp6I CCWGG 9 cut(s) 696, 718, 797, 988, 1003, 1077, 1629, 1808, 2271
PspCI CACGTG 3 cut(s) 211, 470, 1453
PspFI CCCAGC 1 cut(s) 809
PspGI CCWGG 9 cut(s) 696, 718, 797, 988, 1003, 1077, 1629, 1808, 2271
PspOMI GGGCCC 1 cut(s) 564
PspPPI RGGWCCY 1 cut(s) 1075
PstI CTGCAG 1 cut(s) 1028
PstNI CAGNNNCTG 1 cut(s) 495
PsuI RGATCY 1 cut(s) 74
PvuII CAGCTG 1 cut(s) 809
RsaI GTAC 6 cut(s) 606, 637, 869, 1352, 1653, 2315
RsaNI GTAC 6 cut(s) 605, 636, 868, 1351, 1652, 2314
RseI CAYNNNNRTG 3 cut(s) 449, 945, 1302
SacI GAGCTC 1 cut(s) 2016
SatI GCNGC 7 cut(s) 169, 205, 460, 496, 1024, 1331, 1551
Sau3AI GATC 4 cut(s) 74, 237, 2055, 2220
SchI GAGTC 6 cut(s) 203, 344, 427, 764, 1586, 2120
SduI GDGCHC 8 cut(s) 318, 360, 568, 666, 716, 1461, 1802, 2016
SexAI ACCWGGT 1 cut(s) 1629
SfaNI GCATC 6 cut(s) 321, 488, 634, 999, 1562, 1649
SfcI CTRYAG 1 cut(s) 1024
SgrAI CRCCGGYG 1 cut(s) 2029
SinI GGWCC 7 cut(s) 214, 288, 392, 727, 1075, 1574, 1647
SmiMI CAYNNNNRTG 3 cut(s) 449, 945, 1302
SmlI CTYRAG 4 cut(s) 1214, 1622, 1730, 1994
SmoI CTYRAG 4 cut(s) 1214, 1622, 1730, 1994
SphI GCATGC 2 cut(s) 959, 1330
SsiI CCGC 8 cut(s) 205, 283, 459, 516, 1082, 1239, 1788, 2100
SspMI CTAG 5 cut(s) 198, 590, 1323, 1683, 1860
SstI GAGCTC 1 cut(s) 2016
StyI CCWWGG 3 cut(s) 175, 1375, 2279
TaaI ACNGT 2 cut(s) 604, 2023
TaqI TCGA 6 cut(s) 183, 236, 464, 482, 1875, 2223
TauI GCSGC 2 cut(s) 207, 462
TfiI GAWTC 5 cut(s) 373, 1153, 1882, 1894, 2138
TscAI CASTG 6 cut(s) 115, 780, 1281, 1466, 1762, 2101
TseFI GTSAC 5 cut(s) 48, 141, 1448, 1589, 1846
TseI GCWGC 5 cut(s) 168, 495, 1023, 1330, 1550
Tsp45I GTSAC 5 cut(s) 48, 141, 1448, 1589, 1846
TspDTI ATGAA 5 cut(s) 235, 566, 2148, 2180, 2234
TspGWI ACGGA 1 cut(s) 1639
TspRI CASTG 6 cut(s) 115, 780, 1281, 1466, 1762, 2101
Van91I CCANNNNNTGG 1 cut(s) 285
Vha464I CTTAAG 1 cut(s) 1622
VneI GTGCAC 2 cut(s) 662, 1457
VpaK11BI GGWCC 7 cut(s) 214, 288, 392, 727, 1075, 1574, 1647
VspI ATTAAT 2 cut(s) 24, 996
XagI CCTNNNNNAGG 1 cut(s) 701
XapI RAATTY 3 cut(s) 857, 923, 2120
XceI RCATGY 5 cut(s) 944, 959, 1330, 1516, 2089
XmnI GAANNNNTTC 1 cut(s) 1298
XspI CTAG 5 cut(s) 198, 590, 1323, 1683, 1860
Zsp2I ATGCAT 1 cut(s) 649
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.