pycom14g15030
NAC Family

inactive poly ADP-ribose polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0007192
Physical Location & Seq
Forward (+)
17701562 .. 17703747
2186 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1098 bp
ATGGAGTACAGCAATGATGATACGAATCTTTCTTCTGGAAATTCATCGGAAGACGAACTCAGCGATCAATTCACCGATTCGGGTACCACCGACTCTGCCTCAGAGACCGTCCAGTCGTCCATCTCCGACTGTGAAAGCGTTGTCGATTCGGGTTCTTCAAGTTCCCGGAATCAACCTCGGCCATTCGGTGATGGGTTGGTCCCGCTTTTCGACGGGGACAGAGTTCACGACCTTATCAAGCAGCGGTTCGTTTCGAGTTTGGGATTGCTTGGGACCCAAGTAAGTGTTGTGGCGGTTCACAGAAACTCCTACTCGAGCCTCGTGGGTCAAGCAAGGCTACGTTCGTTTCAAATTTACTTGAAAGCGGTGGAGGAGAAGTGCGGGGGAAATCCCAACATGAAGTACGCTTGGTACGCGCCCTATTCGAAAGATGAGATTTCGAAGATCATATGCCATGGTTTCGGAAACTTTGAGCAGCAACAGAGAAATGGGGTGTATGGCAATGGTGTTTATCTTGCTCCCGATGATTCTCCTATGCAATGTTTGGAAAGCTTGAATGTTGACGAAGACGGTTTACGCCATCTTTTGCTCTGCCGTGTCATATTGGGGAGGCCGGAGGTTGTGTGCCCTGGCTCTAAACAGCTTCATCCGAGCTCCGAACAGTTCGATTCGGGCATCGACAGCATCTTTGCTCCCAAAAAGTACATAGTTTGGAGTACACACATGAACACTCACATCTTGCCGGAGTATGTCATCAGTTTCAGAGCTCCTAATTGCTTGAAAGATTTCTTGAAGATTCAAGAACCAATCAAGAAACCGACTTCACCGTGGATGCCATTTCAGGCGCTAATCGGCGTGCTTTCGAAGTTCTTGCCTCCACCTACCATTGCCTTGATCAGCAAGTATTACAAAGAACACAGAGCGAATGTGATCACAAGGCAACAACTAATACAAAAAGTGAGACAAATTGCAGGAGACGAGTTGCTGGTTTCAATTATCAAATCTTTTAGAGCCAAGCAAATCAAACCACTGCAAAGGGTTGCTCAAAATGGAGGCAGGAGTAGGATGGACGGTGTAGATGCGCCAATTTTCTTTTGA

Protein Analysis

366

Amino Acids

40.67

Weight (kDa)

6.71

Isoelectric Point (pI)

46.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RST PF12174 272 - 337 1.1e-25 RCD1-SRO-TAF4 (RST) plant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 112
Acc65I GGTACC 1 cut(s) 83
AccB1I GGYRCC 1 cut(s) 83
AccII CGCG 1 cut(s) 416
AciI CCGC 5 cut(s) 203, 244, 293, 365, 381
AcoI YGGCCR 1 cut(s) 179
AcsI RAATTY 2 cut(s) 40, 351
AfaI GTAC 6 cut(s) 8, 85, 404, 413, 704, 718
AgsI TTSAA 8 cut(s) 159, 350, 361, 556, 781, 793, 800, 993
AjnI CCWGG 1 cut(s) 628
AluBI AGCT 4 cut(s) 552, 643, 654, 767
AluI AGCT 4 cut(s) 552, 643, 654, 767
Alw21I GWGCWC 2 cut(s) 656, 769
Alw26I GTCTC 3 cut(s) 98, 955, 969
Ama87I CYCGRG 1 cut(s) 313
AoxI GGCC 2 cut(s) 179, 611
ApeKI GCWGC 2 cut(s) 241, 475
ApoI RAATTY 2 cut(s) 40, 351
ArsI GACNNNNNNTTYG 2 cut(s) 671, 703
Asp700I GAANNNNTTC 1 cut(s) 785
Asp718I GGTACC 1 cut(s) 83
AspLEI GCGC 3 cut(s) 418, 847, 1084
AspS9I GGNCC 2 cut(s) 199, 273
AsuC2I CCSGG 1 cut(s) 166
AsuHPI GGTGA 3 cut(s) 64, 200, 816
AsuII TTCGAA 3 cut(s) 425, 440, 863
AvaI CYCGRG 1 cut(s) 313
AvaII GGWCC 2 cut(s) 199, 273
BaeGI GKGCMC 1 cut(s) 629
BanI GGYRCC 1 cut(s) 83
BanII GRGCYC 2 cut(s) 656, 769
BarI GAAGNNNNNNTAC 2 cut(s) 558, 590
BauI CACGAG 1 cut(s) 320
BbsI GAAGAC 2 cut(s) 57, 573
Bbv12I GWGCWC 2 cut(s) 656, 769
BbvI GCAGC 2 cut(s) 253, 487
BccI CCATC 4 cut(s) 128, 185, 588, 1060
BceAI ACGGC 1 cut(s) 579
BcgI CGANNNNNNTGC 2 cut(s) 853, 887
BciT130I CCWGG 1 cut(s) 630
BclI TGATCA 2 cut(s) 894, 930
BcnI CCSGG 1 cut(s) 166
BcoDI GTCTC 3 cut(s) 98, 955, 969
BfoI RGCGCY 1 cut(s) 848
BisI GCNGC 2 cut(s) 242, 476
BlsI GCNGC 2 cut(s) 243, 477
Bme1390I CCNGG 2 cut(s) 166, 630
Bme18I GGWCC 2 cut(s) 199, 273
BmeT110I CYCGRG 1 cut(s) 313
BmgT120I GGNCC 2 cut(s) 199, 273
BmiI GGNNCC 4 cut(s) 85, 201, 274, 275
BmrFI CCNGG 2 cut(s) 166, 630
BmsI GCATC 4 cut(s) 684, 693, 822, 1069
BpiI GAAGAC 2 cut(s) 57, 573
Bpu14I TTCGAA 3 cut(s) 425, 440, 863
BpuMI CCSGG 1 cut(s) 166
BsaBI GATNNNNATC 1 cut(s) 24
BsaI GGTCTC 1 cut(s) 98
BsaJI CCNNGG 4 cut(s) 176, 454, 628, 827
BsaXI ACNNNNNCTCC 6 cut(s) 290, 320, 362, 392, 608, 638
Bse1I ACTGG 1 cut(s) 112
Bse3DI GCAATG 4 cut(s) 19, 508, 545, 885
Bse8I GATNNNNATC 1 cut(s) 24
BseBI CCWGG 1 cut(s) 630
BseDI CCNNGG 4 cut(s) 176, 454, 628, 827
BseGI GGATG 3 cut(s) 646, 837, 1071
BseJI GATNNNNATC 1 cut(s) 24
BseMI GCAATG 4 cut(s) 19, 508, 545, 885
BseMII CTCAG 2 cut(s) 73, 114
BseNI ACTGG 1 cut(s) 112
BseRI GAGGAG 1 cut(s) 386
BseSI GKGCMC 1 cut(s) 629
BseXI GCAGC 2 cut(s) 253, 487
Bsh1236I CGCG 1 cut(s) 416
BshFI GGCC 2 cut(s) 181, 613
BshNI GGYRCC 1 cut(s) 83
BsiHKAI GWGCWC 2 cut(s) 656, 769
BsiHKCI CYCGRG 1 cut(s) 313
BsiSI CCGG 3 cut(s) 166, 614, 743
BslFI GGGAC 3 cut(s) 185, 230, 286
BsmAI GTCTC 3 cut(s) 98, 955, 969
BsmBI CGTCTC 1 cut(s) 969
BsmFI GGGAC 3 cut(s) 185, 230, 286
BsnI GGCC 2 cut(s) 181, 613
Bso31I GGTCTC 1 cut(s) 98
BsoBI CYCGRG 1 cut(s) 313
Bsp119I TTCGAA 3 cut(s) 425, 440, 863
Bsp1286I GDGCHC 3 cut(s) 629, 656, 769
Bsp143I GATC 4 cut(s) 64, 444, 894, 930
Bsp19I CCATGG 1 cut(s) 454
BspACI CCGC 5 cut(s) 203, 244, 293, 365, 381
BspANI GGCC 2 cut(s) 181, 613
BspCNI CTCAG 2 cut(s) 72, 113
BspFNI CGCG 1 cut(s) 416
BspLI GGNNCC 4 cut(s) 85, 201, 274, 275
BspT104I TTCGAA 3 cut(s) 425, 440, 863
BspT107I GGYRCC 1 cut(s) 83
BspTNI GGTCTC 1 cut(s) 98
BsrDI GCAATG 4 cut(s) 19, 508, 545, 885
BsrI ACTGG 1 cut(s) 112
BssECI CCNNGG 4 cut(s) 176, 454, 628, 827
BssMI GATC 4 cut(s) 64, 444, 894, 930
BssSI CACGAG 1 cut(s) 320
BssT1I CCWWGG 1 cut(s) 454
Bst2BI CACGAG 1 cut(s) 320
Bst2UI CCWGG 1 cut(s) 630
Bst4CI ACNGT 6 cut(s) 109, 131, 572, 663, 828, 1073
BstBI TTCGAA 3 cut(s) 425, 440, 863
BstC8I GCNNGC 1 cut(s) 857
BstDEI CTNAG 2 cut(s) 59, 100
BstDSI CCRYGG 2 cut(s) 454, 827
BstF5I GGATG 3 cut(s) 646, 837, 1071
BstFNI CGCG 1 cut(s) 416
BstH2I RGCGCY 1 cut(s) 848
BstHHI GCGC 3 cut(s) 418, 847, 1084
BstKTI GATC 4 cut(s) 67, 447, 897, 933
BstMAI GTCTC 3 cut(s) 98, 955, 969
BstMBI GATC 4 cut(s) 64, 444, 894, 930
BstMWI GCNNNNNNNGC 2 cut(s) 413, 681
BstNI CCWGG 1 cut(s) 630
BstSCI CCNGG 2 cut(s) 164, 628
BstSLI GKGCMC 1 cut(s) 629
BstUI CGCG 1 cut(s) 416
BstV1I GCAGC 2 cut(s) 253, 487
BstV2I GAAGAC 2 cut(s) 57, 573
BsuRI GGCC 2 cut(s) 181, 613
BtgI CCRYGG 2 cut(s) 454, 827
BtsCI GGATG 3 cut(s) 646, 837, 1071
BtsI GCAGTG 1 cut(s) 1028
BtsIMutI CAGTG 1 cut(s) 1028
Cac8I GCNNGC 1 cut(s) 857
CfoI GCGC 3 cut(s) 418, 847, 1084
Cfr13I GGNCC 2 cut(s) 199, 273
Csp6I GTAC 6 cut(s) 7, 84, 403, 412, 703, 717
CviAII CATG 3 cut(s) 397, 455, 724
CviQI GTAC 6 cut(s) 7, 84, 403, 412, 703, 717
DdeI CTNAG 2 cut(s) 59, 100
DpnI GATC 4 cut(s) 66, 446, 896, 932
DpnII GATC 4 cut(s) 64, 444, 894, 930
DrdI GACNNNNNNGTC 1 cut(s) 112
DseDI GACNNNNNNGTC 1 cut(s) 112
EaeI YGGCCR 1 cut(s) 179
Ecl136II GAGCTC 2 cut(s) 654, 767
Eco130I CCWWGG 1 cut(s) 454
Eco24I GRGCYC 2 cut(s) 656, 769
Eco31I GGTCTC 1 cut(s) 98
Eco47I GGWCC 2 cut(s) 199, 273
Eco53kI GAGCTC 2 cut(s) 654, 767
Eco88I CYCGRG 1 cut(s) 313
EcoICRI GAGCTC 2 cut(s) 654, 767
EcoO109I RGGNCCY 1 cut(s) 273
EcoRII CCWGG 1 cut(s) 628
EcoT14I CCWWGG 1 cut(s) 454
EcoT38I GRGCYC 2 cut(s) 656, 769
ErhI CCWWGG 1 cut(s) 454
Esp3I CGTCTC 1 cut(s) 969
FaeI CATG 3 cut(s) 400, 458, 727
FaqI GGGAC 3 cut(s) 185, 230, 286
FatI CATG 3 cut(s) 396, 454, 723
FauI CCCGC 2 cut(s) 210, 374
FauNDI CATATG 1 cut(s) 449
FbaI TGATCA 2 cut(s) 894, 930
Fnu4HI GCNGC 2 cut(s) 242, 476
FokI GGATG 3 cut(s) 633, 844, 1078
FriOI GRGCYC 2 cut(s) 656, 769
Fsp4HI GCNGC 2 cut(s) 242, 476
GlaI GCGC 3 cut(s) 417, 846, 1083
GluI GCNGC 2 cut(s) 242, 476
HaeII RGCGCY 1 cut(s) 848
HaeIII GGCC 2 cut(s) 181, 613
HapII CCGG 3 cut(s) 166, 614, 743
HhaI GCGC 3 cut(s) 418, 847, 1084
Hin1II CATG 3 cut(s) 400, 458, 727
Hin6I GCGC 3 cut(s) 416, 845, 1082
HinP1I GCGC 3 cut(s) 416, 845, 1082
HincII GTYRAC 1 cut(s) 562
HindII GTYRAC 1 cut(s) 562
HindIII AAGCTT 1 cut(s) 550
HinfI GANTC 8 cut(s) 25, 77, 92, 146, 169, 527, 668, 796
HpaII CCGG 3 cut(s) 166, 614, 743
HphI GGTGA 3 cut(s) 64, 200, 816
Hpy166II GTNNAC 5 cut(s) 226, 298, 562, 575, 719
Hpy188I TCNGA 7 cut(s) 49, 103, 127, 464, 651, 658, 764
Hpy188III TCNNGA 6 cut(s) 36, 227, 521, 790, 800, 811
Hpy8I GTNNAC 5 cut(s) 226, 298, 562, 575, 719
Hpy99I CGWCG 1 cut(s) 215
HpyCH4III ACNGT 6 cut(s) 109, 131, 572, 663, 828, 1073
HpyCH4IV ACGT 1 cut(s) 340
HpyCH4V TGCA 3 cut(s) 538, 971, 1033
HpyF10VI GCNNNNNNNGC 2 cut(s) 413, 681
HpyF3I CTNAG 2 cut(s) 59, 100
HpySE526I ACGT 1 cut(s) 340
Hsp92II CATG 3 cut(s) 400, 458, 727
HspAI GCGC 3 cut(s) 416, 845, 1082
KflI GGGWCCC 1 cut(s) 273
KpnI GGTACC 1 cut(s) 87
Ksp22I TGATCA 2 cut(s) 894, 930
Kzo9I GATC 4 cut(s) 64, 444, 894, 930
LmnI GCTCC 4 cut(s) 523, 659, 697, 772
Lsp1109I GCAGC 2 cut(s) 253, 487
LweI GCATC 4 cut(s) 684, 693, 822, 1069
MaeII ACGT 1 cut(s) 340
MalI GATC 4 cut(s) 66, 446, 896, 932
MboI GATC 4 cut(s) 64, 444, 894, 930
MboII GAAGA 6 cut(s) 24, 62, 147, 454, 578, 805
MhlI GDGCHC 3 cut(s) 629, 656, 769
MluCI AATT 7 cut(s) 40, 68, 351, 772, 966, 993, 1086
MlyI GAGTC 1 cut(s) 86
MmeI TCCRAC 1 cut(s) 150
MnlI CCTC 8 cut(s) 109, 186, 329, 364, 603, 610, 885, 1046
MroXI GAANNNNTTC 1 cut(s) 785
MspA1I CMGCKG 1 cut(s) 244
MspI CCGG 3 cut(s) 166, 614, 743
MspR9I CCNGG 2 cut(s) 166, 630
MvaI CCWGG 1 cut(s) 630
MvnI CGCG 1 cut(s) 416
MwoI GCNNNNNNNGC 2 cut(s) 413, 681
NciI CCSGG 1 cut(s) 166
NcoI CCATGG 1 cut(s) 454
NdeI CATATG 1 cut(s) 449
NdeII GATC 4 cut(s) 64, 444, 894, 930
NlaIII CATG 3 cut(s) 400, 458, 727
NlaIV GGNNCC 4 cut(s) 85, 201, 274, 275
NmeAIII GCCGAG 1 cut(s) 157
NspV TTCGAA 3 cut(s) 425, 440, 863
PaeR7I CTCGAG 1 cut(s) 313
PcsI WCGNNNNNNNCGW 1 cut(s) 60
PdmI GAANNNNTTC 1 cut(s) 785
PfeI GAWTC 7 cut(s) 25, 77, 146, 169, 527, 668, 796
PfoI TCCNGGA 1 cut(s) 164
PkrI GCNGC 2 cut(s) 243, 477
PleI GAGTC 1 cut(s) 86
PpsI GAGTC 1 cut(s) 86
PpuMI RGGWCCY 1 cut(s) 273
Psp124BI GAGCTC 2 cut(s) 656, 769
Psp5II RGGWCCY 1 cut(s) 273
Psp6I CCWGG 1 cut(s) 628
PspGI CCWGG 1 cut(s) 628
PspN4I GGNNCC 4 cut(s) 85, 201, 274, 275
PspPI GGNCC 2 cut(s) 199, 273
PspPPI RGGWCCY 1 cut(s) 273
PspXI VCTCGAGB 1 cut(s) 313
RsaI GTAC 6 cut(s) 8, 85, 404, 413, 704, 718
RsaNI GTAC 6 cut(s) 7, 84, 403, 412, 703, 717
SacI GAGCTC 2 cut(s) 656, 769
SatI GCNGC 2 cut(s) 242, 476
Sau3AI GATC 4 cut(s) 64, 444, 894, 930
Sau96I GGNCC 2 cut(s) 199, 273
SchI GAGTC 1 cut(s) 86
ScrFI CCNGG 2 cut(s) 166, 630
SduI GDGCHC 3 cut(s) 629, 656, 769
SetI ASST 9 cut(s) 178, 234, 343, 554, 621, 645, 656, 769, 883
SfaNI GCATC 4 cut(s) 684, 693, 822, 1069
Sfr274I CTCGAG 1 cut(s) 313
SfuI TTCGAA 3 cut(s) 425, 440, 863
SinI GGWCC 2 cut(s) 199, 273
SlaI CTCGAG 1 cut(s) 313
SmlI CTYRAG 1 cut(s) 313
SmoI CTYRAG 1 cut(s) 313
Sse9I AATT 7 cut(s) 40, 68, 351, 772, 966, 993, 1086
SsiI CCGC 5 cut(s) 203, 244, 293, 365, 381
SstI GAGCTC 2 cut(s) 656, 769
StyD4I CCNGG 2 cut(s) 164, 628
StyI CCWWGG 1 cut(s) 454
TaaI ACNGT 6 cut(s) 109, 131, 572, 663, 828, 1073
TaiI ACGT 1 cut(s) 343
TaqI TCGA 9 cut(s) 144, 210, 254, 314, 425, 440, 666, 678, 863
TasI AATT 7 cut(s) 40, 68, 351, 772, 966, 993, 1086
TatI WGTACW 3 cut(s) 6, 702, 716
TfiI GAWTC 7 cut(s) 25, 77, 146, 169, 527, 668, 796
TscAI CASTG 1 cut(s) 1035
TseI GCWGC 2 cut(s) 241, 475
TspDTI ATGAA 4 cut(s) 33, 413, 635, 740
TspRI CASTG 1 cut(s) 1035
VpaK11BI GGWCC 2 cut(s) 199, 273
XapI RAATTY 2 cut(s) 40, 351
XhoI CTCGAG 1 cut(s) 313
XmnI GAANNNNTTC 1 cut(s) 785
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.