pycom14g15890

protein At5g50100, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0010102
Physical Location & Seq
Forward (+)
18353915 .. 18360646
6732 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 678 bp
ATGGCAATGAAAGCAGCTGCTTCCGCATGTAAAATTGGAGGGAGACAAAGAAACCCTTCTCTCTCATCAATTCCTCTCTCTCTCCAACAACTCAAAGTTCATCAGATTCATCATCCCCAAAGATTCATCTCAGCTCCTATTCACCAACATGGGTTTAAGTCTTCAACAAGGGCTATTAGCGGGGCAGCTGCAGACCCCATGACTCCAAATACAAAAAATGGTCAAGAACAAACACCTCAGAATTGGAAGATTAAAATGCTCTTTGATGGGGATTGCCCTTTATGCATGCGAGAGGTGGACATGCTGCGAGAGAGAAATAAGCGTTATGGTACTATCAAGTTTGTTGACATAAGTTCAGATGATTACTCCCCGGAGGAGAATCAGGGGCTGGACTACAAGACTGTTATGGGAAACATTCATGCCATCCTCTCTGATGGAACTGTAGTCACTGATGTCGAAGCATTCAGAAAGCTATACGAACAGGTGGGGCTCGGATGGGTATATGCCATAACTAAATACGAGCCTATTGCAACAATAGCAGGTGCTGTATATGGCGTTTGGGCTAAATATCGTCTTCAAATCACAGGCCGGCCACCTCTAGAAGAGGTTTTGGAGTTGCGAAAGAAGAACAAGGGAGAAGTATGTAAAGATCCAAAAGATTGTAAACTCCCAGAGTAA

Protein Analysis

226

Amino Acids

25.22

Weight (kDa)

9.01

Isoelectric Point (pI)

35.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DCC1-like PF04134 87 - 197 4e-25 DCC1-like thiol-disulfide oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 530
Acc36I ACCTGC 1 cut(s) 530
AciI CCGC 2 cut(s) 24, 180
AclWI GGATC 1 cut(s) 644
AcoI YGGCCR 1 cut(s) 590
AfaI GTAC 1 cut(s) 331
AgsI TTSAA 2 cut(s) 165, 578
AluBI AGCT 4 cut(s) 17, 134, 188, 472
AluI AGCT 4 cut(s) 17, 134, 188, 472
Alw26I GTCTC 1 cut(s) 37
AlwI GGATC 1 cut(s) 644
AlwNI CAGNNNCTG 2 cut(s) 388, 545
AoxI GGCC 2 cut(s) 586, 590
ApeKI GCWGC 5 cut(s) 14, 17, 185, 188, 304
Asp700I GAANNNNTTC 1 cut(s) 55
AsuC2I CCSGG 1 cut(s) 371
AsuHPI GGTGA 1 cut(s) 134
BanII GRGCYC 1 cut(s) 492
BbsI GAAGAC 2 cut(s) 153, 566
BbvI GCAGC 5 cut(s) 4, 26, 175, 197, 291
BccI CCATC 4 cut(s) 260, 428, 431, 489
BcgI CGANNNNNNTGC 2 cut(s) 509, 543
BcnI CCSGG 1 cut(s) 371
BcoDI GTCTC 1 cut(s) 37
BfaI CTAG 1 cut(s) 599
BfmI CTRYAG 2 cut(s) 189, 441
BfuAI ACCTGC 1 cut(s) 530
BisI GCNGC 5 cut(s) 15, 18, 186, 189, 305
BlsI GCNGC 5 cut(s) 16, 19, 187, 190, 306
Bme1390I CCNGG 1 cut(s) 371
BmrFI CCNGG 1 cut(s) 371
BpiI GAAGAC 2 cut(s) 153, 566
BpuMI CCSGG 1 cut(s) 371
BsaJI CCNNGG 1 cut(s) 369
Bse118I RCCGGY 1 cut(s) 588
Bse3DI GCAATG 1 cut(s) 12
BseDI CCNNGG 1 cut(s) 369
BseGI GGATG 3 cut(s) 112, 423, 500
BseMI GCAATG 1 cut(s) 12
BseMII CTCAG 2 cut(s) 144, 251
BseRI GAGGAG 1 cut(s) 389
BseXI GCAGC 5 cut(s) 4, 26, 175, 197, 291
BshFI GGCC 2 cut(s) 588, 592
BsiSI CCGG 2 cut(s) 371, 589
BsmAI GTCTC 1 cut(s) 37
BsmI GAATGC 1 cut(s) 461
BsnI GGCC 2 cut(s) 588, 592
Bsp1286I GDGCHC 1 cut(s) 492
Bsp143I GATC 1 cut(s) 649
BspACI CCGC 2 cut(s) 24, 180
BspANI GGCC 2 cut(s) 588, 592
BspCNI CTCAG 2 cut(s) 143, 250
BspMAI CTGCAG 1 cut(s) 193
BspMI ACCTGC 1 cut(s) 530
BspPI GGATC 1 cut(s) 644
BsrDI GCAATG 1 cut(s) 12
BsrFI RCCGGY 1 cut(s) 588
BssAI RCCGGY 1 cut(s) 588
BssECI CCNNGG 1 cut(s) 369
BssMI GATC 1 cut(s) 649
Bst4CI ACNGT 2 cut(s) 403, 442
Bst6I CTCTTC 1 cut(s) 597
BstC8I GCNNGC 2 cut(s) 287, 590
BstDEI CTNAG 2 cut(s) 130, 237
BstF5I GGATG 3 cut(s) 112, 423, 500
BstKTI GATC 1 cut(s) 652
BstMAI GTCTC 1 cut(s) 37
BstMBI GATC 1 cut(s) 649
BstMWI GCNNNNNNNGC 4 cut(s) 11, 23, 282, 536
BstNSI RCATGY 3 cut(s) 30, 289, 304
BstSCI CCNGG 1 cut(s) 369
BstSFI CTRYAG 2 cut(s) 189, 441
BstV1I GCAGC 5 cut(s) 4, 26, 175, 197, 291
BstV2I GAAGAC 2 cut(s) 153, 566
BstX2I RGATCY 1 cut(s) 649
BstYI RGATCY 1 cut(s) 649
BsuRI GGCC 2 cut(s) 588, 592
BtsCI GGATG 3 cut(s) 112, 423, 500
BtsIMutI CAGTG 1 cut(s) 447
BveI ACCTGC 1 cut(s) 530
Cac8I GCNNGC 2 cut(s) 287, 590
CaiI CAGNNNCTG 2 cut(s) 388, 545
Cfr10I RCCGGY 1 cut(s) 588
Csp6I GTAC 1 cut(s) 330
CviAII CATG 6 cut(s) 27, 149, 199, 286, 301, 419
CviQI GTAC 1 cut(s) 330
DdeI CTNAG 2 cut(s) 130, 237
DpnI GATC 1 cut(s) 651
DpnII GATC 1 cut(s) 649
EaeI YGGCCR 1 cut(s) 590
Eam1104I CTCTTC 1 cut(s) 597
EarI CTCTTC 1 cut(s) 597
Eco24I GRGCYC 1 cut(s) 492
EcoT22I ATGCAT 1 cut(s) 287
EcoT38I GRGCYC 1 cut(s) 492
FaeI CATG 6 cut(s) 30, 152, 202, 289, 304, 422
FalI AAGNNNNNCTT 2 cut(s) 40, 72
FatI CATG 6 cut(s) 26, 148, 198, 285, 300, 418
FauI CCCGC 1 cut(s) 173
Fnu4HI GCNGC 5 cut(s) 15, 18, 186, 189, 305
FokI GGATG 3 cut(s) 99, 410, 507
FriOI GRGCYC 1 cut(s) 492
FseI GGCCGGCC 1 cut(s) 592
Fsp4HI GCNGC 5 cut(s) 15, 18, 186, 189, 305
FspBI CTAG 1 cut(s) 599
GluI GCNGC 5 cut(s) 15, 18, 186, 189, 305
HaeIII GGCC 2 cut(s) 588, 592
HapII CCGG 2 cut(s) 371, 589
Hin1II CATG 6 cut(s) 30, 152, 202, 289, 304, 422
HincII GTYRAC 1 cut(s) 346
HindII GTYRAC 1 cut(s) 346
HinfI GANTC 4 cut(s) 106, 123, 202, 379
HpaII CCGG 2 cut(s) 371, 589
HphI GGTGA 1 cut(s) 134
Hpy166II GTNNAC 3 cut(s) 298, 346, 665
Hpy188I TCNGA 6 cut(s) 105, 240, 358, 433, 467, 494
Hpy188III TCNNGA 2 cut(s) 224, 599
Hpy8I GTNNAC 3 cut(s) 298, 346, 665
HpyAV CCTTC 1 cut(s) 66
HpyCH4III ACNGT 2 cut(s) 403, 442
HpyCH4V TGCA 3 cut(s) 191, 285, 530
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 23, 282, 536
HpyF3I CTNAG 2 cut(s) 130, 237
Hsp92II CATG 6 cut(s) 30, 152, 202, 289, 304, 422
KroI GCCGGC 1 cut(s) 588
KroNI GCCGGC 1 cut(s) 590
Kzo9I GATC 1 cut(s) 649
LmnI GCTCC 1 cut(s) 139
LpnPI CCDG 7 cut(s) 368, 374, 384, 467, 525, 570, 602
Lsp1109I GCAGC 5 cut(s) 4, 26, 175, 197, 291
MaeI CTAG 1 cut(s) 599
MaeIII GTNAC 1 cut(s) 445
MalI GATC 1 cut(s) 651
MboI GATC 1 cut(s) 649
MboII GAAGA 5 cut(s) 153, 259, 566, 614, 637
MflI RGATCY 1 cut(s) 649
MhlI GDGCHC 1 cut(s) 492
MluCI AATT 3 cut(s) 33, 69, 241
MlyI GAGTC 1 cut(s) 196
MmeI TCCRAC 1 cut(s) 109
MnlI CCTC 8 cut(s) 32, 84, 246, 286, 367, 437, 598, 606
Mph1103I ATGCAT 1 cut(s) 287
MroNI GCCGGC 1 cut(s) 588
MroXI GAANNNNTTC 1 cut(s) 55
MseI TTAA 2 cut(s) 156, 252
MslI CAYNNNNRTG 1 cut(s) 147
MspA1I CMGCKG 2 cut(s) 17, 188
MspI CCGG 2 cut(s) 371, 589
MspR9I CCNGG 1 cut(s) 371
Mva1269I GAATGC 1 cut(s) 461
MwoI GCNNNNNNNGC 4 cut(s) 11, 23, 282, 536
NaeI GCCGGC 1 cut(s) 590
NciI CCSGG 1 cut(s) 371
NdeII GATC 1 cut(s) 649
NgoMIV GCCGGC 1 cut(s) 588
NlaIII CATG 6 cut(s) 30, 152, 202, 289, 304, 422
NmuCI GTSAC 1 cut(s) 445
NsiI ATGCAT 1 cut(s) 287
NspI RCATGY 3 cut(s) 30, 289, 304
PaeI GCATGC 1 cut(s) 289
PaqCI CACCTGC 1 cut(s) 530
PctI GAATGC 1 cut(s) 461
PdiI GCCGGC 1 cut(s) 590
PdmI GAANNNNTTC 1 cut(s) 55
PfeI GAWTC 3 cut(s) 106, 123, 379
PkrI GCNGC 5 cut(s) 16, 19, 187, 190, 306
PleI GAGTC 1 cut(s) 196
PpsI GAGTC 1 cut(s) 196
PstI CTGCAG 1 cut(s) 193
PstNI CAGNNNCTG 2 cut(s) 388, 545
PsuI RGATCY 1 cut(s) 649
PvuII CAGCTG 2 cut(s) 17, 188
RigI GGCCGGCC 1 cut(s) 592
RsaI GTAC 1 cut(s) 331
RsaNI GTAC 1 cut(s) 330
RseI CAYNNNNRTG 1 cut(s) 147
SaqAI TTAA 2 cut(s) 156, 252
SatI GCNGC 5 cut(s) 15, 18, 186, 189, 305
Sau3AI GATC 1 cut(s) 649
SchI GAGTC 1 cut(s) 196
ScrFI CCNGG 1 cut(s) 371
SduI GDGCHC 1 cut(s) 492
SfcI CTRYAG 2 cut(s) 189, 441
SmiMI CAYNNNNRTG 1 cut(s) 147
SphI GCATGC 1 cut(s) 289
Sse9I AATT 3 cut(s) 33, 69, 241
SsiI CCGC 2 cut(s) 24, 180
SspMI CTAG 1 cut(s) 599
StyD4I CCNGG 1 cut(s) 369
TaaI ACNGT 2 cut(s) 403, 442
TaqI TCGA 1 cut(s) 456
TasI AATT 3 cut(s) 33, 69, 241
TfiI GAWTC 3 cut(s) 106, 123, 379
Tru1I TTAA 2 cut(s) 156, 252
Tru9I TTAA 2 cut(s) 156, 252
TscAI CASTG 1 cut(s) 454
TseFI GTSAC 1 cut(s) 445
TseI GCWGC 5 cut(s) 14, 17, 185, 188, 304
Tsp45I GTSAC 1 cut(s) 445
TspDTI ATGAA 5 cut(s) 23, 89, 98, 115, 407
TspRI CASTG 1 cut(s) 454
XbaI TCTAGA 1 cut(s) 598
XceI RCATGY 3 cut(s) 30, 289, 304
XmnI GAANNNNTTC 1 cut(s) 55
XspI CTAG 1 cut(s) 599
Zsp2I ATGCAT 1 cut(s) 287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.