pycom14g16630

Protein of unknown function (DUF3223)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0014579
Physical Location & Seq
Forward (+)
18855234 .. 18856554
1321 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 561 bp
ATGCCAGAGAAACTCGCCGAAACCTCGGAAGCCACCGCCGCTGATGGCGCTAAACATATGGACGTAGAAACTTCAGAGCCTGCTGCGGCTCAAAATTGCGACGAGGCTGACGCCATTAATGGGGAGGCGGAGGCAAACTCGAAGCGGGTCAGGGACGAGGAGGGGAGTGAAGGAAACGATGCTAAGAAGACGAAGGTGGAGACGTCGCCGGAGGAAGAACGGTTGGAGGAGAAGTTAGGGGAAGGGGAGAAGTCGGGTCGGGTCAGCTTGGGTCCGAAGAGTTTTGGGTCGTCGGTGGAGATGTTCGACTACTTCTACAAATTCCTCCATTACTGGCCTACTGATCTCAATGTCAATAAGTATGAGTATCTGGTTTTGCTCGACTTGCTTAAGAAGGGTCATGCCGAACCCGATAAGAAGATTGGTGGAGGGGTCAAGGCATTCCAAGTTCGGACCCATCCGTTGTATAAAAGCAGGTGCTTCTTCCTCATTAGGGAAGATGAAGTTGTTGATGATTTTAGCTTCAGGAAGTGTGATCGATCAGATACTTCCCTTGCCTGA

Protein Analysis

187

Amino Acids

20.84

Weight (kDa)

4.89

Isoelectric Point (pI)

34.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3223 PF11523 107 - 178 1.6e-24 Protein of unknown function (DUF3223)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014579)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G62440
fragaria_vesca FvH4_5g14920
malus_domestica MD06G1193500.v1.1 MD14G1200600.v1.1
prunus_persica Prupe.5G193800_v2.0.a1
pyrus_communis pycom06g17160 pycom14g16630
rosa_chinensis RchiOBHm_Chr7g0181461
rosa_laevigata RLG00000005192
rosa_multiflora Rmu_sc0004621.1_g000022
rosa_roxburghii Rroxscaffold_3G00271980
rosa_rugosa Rorug06G0441500
rosa_samantha Rh7AG043300 Rh7BG042700 Rh7CG044500 Rh7DG043100
rosa_wichuraiana Rw7G003600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 465
AatII GACGTC 1 cut(s) 206
Acc36I ACCTGC 1 cut(s) 465
AciI CCGC 5 cut(s) 36, 39, 86, 128, 145
AcsI RAATTY 1 cut(s) 320
AcuI CTGAAG 2 cut(s) 57, 508
AcyI GRCGYC 2 cut(s) 111, 203
AfiI CCNNNNNNNGG 2 cut(s) 120, 493
AflII CTTAAG 1 cut(s) 389
AluBI AGCT 2 cut(s) 267, 522
AluI AGCT 2 cut(s) 267, 522
Alw26I GTCTC 1 cut(s) 194
AlwNI CAGNNNCTG 1 cut(s) 80
AoxI GGCC 1 cut(s) 335
ApeKI GCWGC 1 cut(s) 83
ApoI RAATTY 1 cut(s) 320
AseI ATTAAT 1 cut(s) 117
AspLEI GCGC 1 cut(s) 50
AspS9I GGNCC 2 cut(s) 272, 453
AvaII GGWCC 2 cut(s) 272, 453
BbsI GAAGAC 1 cut(s) 194
BbvI GCAGC 1 cut(s) 70
BccI CCATC 2 cut(s) 38, 465
BcoDI GTCTC 1 cut(s) 194
BfoI RGCGCY 1 cut(s) 51
BfrI CTTAAG 1 cut(s) 389
BfuAI ACCTGC 1 cut(s) 465
BisI GCNGC 3 cut(s) 39, 84, 87
BlsI GCNGC 3 cut(s) 40, 85, 88
Bme18I GGWCC 2 cut(s) 272, 453
BmgT120I GGNCC 2 cut(s) 272, 453
BmiI GGNNCC 2 cut(s) 273, 455
BmsI GCATC 1 cut(s) 169
BpiI GAAGAC 1 cut(s) 194
BplI GAGNNNNNCTC 2 cut(s) 122, 154
Bsa29I ATCGAT 1 cut(s) 538
BsaHI GRCGYC 2 cut(s) 111, 203
BsaJI CCNNGG 1 cut(s) 24
BsaXI ACNNNNNCTCC 2 cut(s) 218, 248
Bsc4I CCNNNNNNNGG 2 cut(s) 120, 493
Bse1I ACTGG 1 cut(s) 338
BseCI ATCGAT 1 cut(s) 538
BseDI CCNNGG 1 cut(s) 24
BseGI GGATG 1 cut(s) 457
BseLI CCNNNNNNNGG 2 cut(s) 120, 493
BseNI ACTGG 1 cut(s) 338
BseRI GAGGAG 2 cut(s) 173, 242
BseXI GCAGC 1 cut(s) 70
BshFI GGCC 1 cut(s) 337
BshVI ATCGAT 1 cut(s) 538
BsiSI CCGG 1 cut(s) 209
BslFI GGGAC 1 cut(s) 167
BslI CCNNNNNNNGG 2 cut(s) 120, 493
BsmAI GTCTC 1 cut(s) 194
BsmBI CGTCTC 1 cut(s) 194
BsmFI GGGAC 1 cut(s) 167
BsmI GAATGC 1 cut(s) 440
BsnI GGCC 1 cut(s) 337
Bsp143I GATC 3 cut(s) 343, 535, 539
BspACI CCGC 5 cut(s) 36, 39, 86, 128, 145
BspANI GGCC 1 cut(s) 337
BspDI ATCGAT 1 cut(s) 538
BspLI GGNNCC 2 cut(s) 273, 455
BspMI ACCTGC 1 cut(s) 465
BspTI CTTAAG 1 cut(s) 389
BsrI ACTGG 1 cut(s) 338
BssECI CCNNGG 1 cut(s) 24
BssMI GATC 3 cut(s) 343, 535, 539
BssNI GRCGYC 2 cut(s) 111, 203
Bst4CI ACNGT 1 cut(s) 222
Bst6I CTCTTC 1 cut(s) 272
BstACI GRCGYC 2 cut(s) 111, 203
BstAFI CTTAAG 1 cut(s) 389
BstC8I GCNNGC 1 cut(s) 81
BstDEI CTNAG 1 cut(s) 183
BstF5I GGATG 1 cut(s) 457
BstH2I RGCGCY 1 cut(s) 51
BstHHI GCGC 1 cut(s) 50
BstKTI GATC 3 cut(s) 346, 538, 542
BstMAI GTCTC 1 cut(s) 194
BstMBI GATC 3 cut(s) 343, 535, 539
BstMWI GCNNNNNNNGC 3 cut(s) 38, 47, 385
BstV1I GCAGC 1 cut(s) 70
BstV2I GAAGAC 1 cut(s) 194
Bsu15I ATCGAT 1 cut(s) 538
BsuRI GGCC 1 cut(s) 337
BsuTUI ATCGAT 1 cut(s) 538
BtsCI GGATG 1 cut(s) 457
BveI ACCTGC 1 cut(s) 465
Cac8I GCNNGC 1 cut(s) 81
CaiI CAGNNNCTG 1 cut(s) 80
CfoI GCGC 1 cut(s) 50
Cfr13I GGNCC 2 cut(s) 272, 453
ClaI ATCGAT 1 cut(s) 538
CseI GACGC 1 cut(s) 119
CviAII CATG 1 cut(s) 401
CviJI RGCY 7 cut(s) 32, 79, 89, 107, 267, 337, 522
CviKI_1 RGCY 7 cut(s) 32, 79, 89, 107, 267, 337, 522
DdeI CTNAG 1 cut(s) 183
DpnI GATC 3 cut(s) 345, 537, 541
DpnII GATC 3 cut(s) 343, 535, 539
Eam1104I CTCTTC 1 cut(s) 272
EarI CTCTTC 1 cut(s) 272
EciI GGCGGA 1 cut(s) 143
Eco47I GGWCC 2 cut(s) 272, 453
Eco57I CTGAAG 2 cut(s) 57, 508
Esp3I CGTCTC 1 cut(s) 194
FaeI CATG 1 cut(s) 404
FaiI YATR 5 cut(s) 57, 59, 363, 402, 468
FaqI GGGAC 1 cut(s) 167
FatI CATG 1 cut(s) 400
FauI CCCGC 1 cut(s) 138
FauNDI CATATG 1 cut(s) 57
Fnu4HI GCNGC 3 cut(s) 39, 84, 87
FokI GGATG 1 cut(s) 444
Fsp4HI GCNGC 3 cut(s) 39, 84, 87
GlaI GCGC 1 cut(s) 49
GluI GCNGC 3 cut(s) 39, 84, 87
HaeII RGCGCY 1 cut(s) 51
HaeIII GGCC 1 cut(s) 337
HapII CCGG 1 cut(s) 209
HgaI GACGC 1 cut(s) 119
HhaI GCGC 1 cut(s) 50
Hin1I GRCGYC 2 cut(s) 111, 203
Hin1II CATG 1 cut(s) 404
Hin6I GCGC 1 cut(s) 48
HinP1I GCGC 1 cut(s) 48
HpaII CCGG 1 cut(s) 209
Hpy188I TCNGA 5 cut(s) 28, 76, 276, 453, 544
Hpy188III TCNNGA 1 cut(s) 526
Hpy99I CGWCG 3 cut(s) 104, 208, 295
HpyAV CCTTC 4 cut(s) 164, 187, 236, 388
HpyCH4III ACNGT 1 cut(s) 222
HpyCH4IV ACGT 2 cut(s) 63, 203
HpyF10VI GCNNNNNNNGC 3 cut(s) 38, 47, 385
HpyF3I CTNAG 1 cut(s) 183
HpySE526I ACGT 2 cut(s) 63, 203
Hsp92I GRCGYC 2 cut(s) 111, 203
Hsp92II CATG 1 cut(s) 404
HspAI GCGC 1 cut(s) 48
Kzo9I GATC 3 cut(s) 343, 535, 539
LpnPI CCDG 8 cut(s) 18, 93, 136, 222, 319, 356, 460, 511
Lsp1109I GCAGC 1 cut(s) 70
LweI GCATC 1 cut(s) 169
MaeII ACGT 2 cut(s) 63, 203
MalI GATC 3 cut(s) 345, 537, 541
MboI GATC 3 cut(s) 343, 535, 539
MboII GAAGA 6 cut(s) 199, 227, 289, 430, 475, 509
MluCI AATT 2 cut(s) 94, 320
MmeI TCCRAC 1 cut(s) 204
MseI TTAA 2 cut(s) 117, 390
MspA1I CMGCKG 1 cut(s) 41
MspCI CTTAAG 1 cut(s) 389
MspI CCGG 1 cut(s) 209
Mva1269I GAATGC 1 cut(s) 440
MwoI GCNNNNNNNGC 3 cut(s) 38, 47, 385
NdeI CATATG 1 cut(s) 57
NdeII GATC 3 cut(s) 343, 535, 539
NlaIII CATG 1 cut(s) 404
NlaIV GGNNCC 2 cut(s) 273, 455
PaqCI CACCTGC 1 cut(s) 465
PctI GAATGC 1 cut(s) 440
PkrI GCNGC 3 cut(s) 40, 85, 88
PshBI ATTAAT 1 cut(s) 117
PspN4I GGNNCC 2 cut(s) 273, 455
PspPI GGNCC 2 cut(s) 272, 453
PstNI CAGNNNCTG 1 cut(s) 80
SaqAI TTAA 2 cut(s) 117, 390
SatI GCNGC 3 cut(s) 39, 84, 87
Sau3AI GATC 3 cut(s) 343, 535, 539
Sau96I GGNCC 2 cut(s) 272, 453
SetI ASST 7 cut(s) 26, 66, 198, 206, 269, 479, 524
SfaNI GCATC 1 cut(s) 169
SinI GGWCC 2 cut(s) 272, 453
SmlI CTYRAG 1 cut(s) 389
SmoI CTYRAG 1 cut(s) 389
Sse9I AATT 2 cut(s) 94, 320
SsiI CCGC 5 cut(s) 36, 39, 86, 128, 145
TaaI ACNGT 1 cut(s) 222
TaiI ACGT 2 cut(s) 66, 206
TaqI TCGA 4 cut(s) 140, 306, 381, 538
TasI AATT 2 cut(s) 94, 320
TauI GCSGC 2 cut(s) 41, 89
Tru1I TTAA 2 cut(s) 117, 390
Tru9I TTAA 2 cut(s) 117, 390
TseI GCWGC 1 cut(s) 83
TspDTI ATGAA 1 cut(s) 516
TspGWI ACGGA 1 cut(s) 450
Vha464I CTTAAG 1 cut(s) 389
VpaK11BI GGWCC 2 cut(s) 272, 453
VspI ATTAAT 1 cut(s) 117
XapI RAATTY 1 cut(s) 320
ZraI GACGTC 1 cut(s) 204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.