pycom14g16650

RWP-RK domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0012997
Physical Location & Seq
Forward (+)
18866109 .. 18867422
1314 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1077 bp
ATGGGTTGGCTGGCCAAGTACGACGTCGTCAATGATCTTGGTGAAGACCCATATTTCTTCCCACATCAACCTTCATTTCCCTCTGTACATGATTTCCGGTTAAATTATATTAGGGGGTATGCATGCGATTGGCAATTTGATTTGCCAATAGTACAAGATCATAGATTTCCTGATCCTCTTCCTTCACTTGAGAGCTGTGTGAATATTGATCCCATTTACTCCTCACTTGACATTCCACCAATCCGAACTGTATTGCAAGGTGTGTTTCAGTATTATGATGATGAAATTTTCTTCGGAAATGGAAACGCGTTGTTTGGAGTTAATTCTAATGATTTATGTGGTGGGTTAACTGAGATTAATCACAATCATCATCAGCAATTTCAACAACAGCAGCAACCTCCATTGCTTCTGACTTGCAAGAACGATGAAAATGGCATTACGGACATGAACGATTCCCTGGAAGAGAGGGATAAGGCGATTAGTGAGAGGCCACACTCTTCTAAAAGACATAGATCATCAAGCTCTTCCCTCTCTTCCTCCAAAATGTTGTCAAGACAGACCATTTCCCAGTACTTCTACATGCCAATCACTCAGGCTGCCAAGGAACTCAACGTAGGGCTAACTCTTCTGAAGAAAAGGTGTCGAGAGTTGGGCATTCGTCGTTGGCCTCATCGGAAGTTGACCAGCCTTCAAACCCTAATCCGAAACATTCAGGTTAATAGGCGTTACATATTTGTACGTGTTCGTGAGTTAGGAAAGGAAGGAGAGGAGGGCGAAGAGAAGCTTCGAAACGCGATCGCGTTGCTGGAGATGGAGAGGAAGCTGCTGGAGGAAGCGCCTGATATGCAGCTTGAGGACAATACGAAGCGTCTAAGGCAGGCATGCTTCAAAGCAAATTACAAGAAGAGGAAGATCATGGGGACGAAGGTTATGGAAAAATCACAATCATCTTTTAGTTGCAGCAACATTCAGTATCCAACAAGTTCGATGGATGCTAACGAGGAAGACGAAGAAATCAAGTCTCTATTGTCTGACTCTTTTTCCTCGAATAACAATGCATGCATCACTCTATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RWP-RK

Protein Analysis

359

Amino Acids

41.39

Weight (kDa)

6.18

Isoelectric Point (pI)

65.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RWP-RK PF02042 183 - 230 4.2e-23 RWP-RK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012997)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18790 AT1G74480
fragaria_vesca FvH4_5g14910
malus_domestica MD06G1193600.v1.1
prunus_persica Prupe.5G193900_v2.0.a1
pyrus_communis pycom14g16650
rosa_chinensis RchiOBHm_Chr7g0181471
rosa_laevigata RLG00000005191
rosa_multiflora Rmu_sc0002060.1_g000022 Rmu_sc0002993.1_g000018 Rmu_sc0004621.1_g000024
rosa_roxburghii Rroxscaffold_3G00271970
rosa_rugosa Rorug06G0441600
rosa_samantha Rh7AG043400 Rh7BG042800 Rh7CG044600 Rh7DG043200
rosa_wichuraiana Rw7G003610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 27
AccII CGCG 3 cut(s) 308, 794, 800
AclWI GGATC 2 cut(s) 167, 203
AcoI YGGCCR 1 cut(s) 12
AcsI RAATTY 1 cut(s) 285
AcuI CTGAAG 1 cut(s) 650
AcyI GRCGYC 1 cut(s) 24
AfaI GTAC 5 cut(s) 20, 87, 153, 572, 738
AflIII ACRYGT 2 cut(s) 306, 739
AgsI TTSAA 3 cut(s) 383, 692, 889
AjnI CCWGG 1 cut(s) 456
AluBI AGCT 5 cut(s) 195, 522, 784, 823, 850
AluI AGCT 5 cut(s) 195, 522, 784, 823, 850
Alw26I GTCTC 1 cut(s) 1026
AlwI GGATC 2 cut(s) 167, 203
AoxI GGCC 3 cut(s) 12, 488, 665
ApeKI GCWGC 5 cut(s) 391, 596, 823, 847, 960
ApoI RAATTY 1 cut(s) 285
AseI ATTAAT 1 cut(s) 357
AsiSI GCGATCGC 1 cut(s) 798
AspLEI GCGC 1 cut(s) 838
AsuHPI GGTGA 1 cut(s) 53
AsuII TTCGAA 1 cut(s) 787
BalI TGGCCA 1 cut(s) 14
BbsI GAAGAC 2 cut(s) 51, 1011
BbvI GCAGC 5 cut(s) 403, 583, 810, 859, 972
BccI CCATC 2 cut(s) 805, 982
BcgI CGANNNNNNTGC 2 cut(s) 784, 818
BciT130I CCWGG 1 cut(s) 458
BciVI GTATCC 1 cut(s) 984
BcoDI GTCTC 1 cut(s) 1026
BfoI RGCGCY 1 cut(s) 839
BfuI GTATCC 1 cut(s) 984
BisI GCNGC 5 cut(s) 392, 597, 824, 848, 961
BlsI GCNGC 5 cut(s) 393, 598, 825, 849, 962
BmcAI AGTACT 1 cut(s) 572
Bme1390I CCNGG 1 cut(s) 458
BmrFI CCNGG 1 cut(s) 458
BmrI ACTGGG 1 cut(s) 562
BmsI GCATC 2 cut(s) 982, 1071
BmuI ACTGGG 1 cut(s) 562
BpiI GAAGAC 2 cut(s) 51, 1011
BpmI CTGGAG 2 cut(s) 827, 848
Bpu14I TTCGAA 1 cut(s) 787
BpuEI CTTGAG 2 cut(s) 209, 872
BsaAI YACGTR 1 cut(s) 740
BsaHI GRCGYC 1 cut(s) 24
BsaJI CCNNGG 2 cut(s) 456, 600
BsaWI WCCGGW 1 cut(s) 96
Bse1I ACTGG 1 cut(s) 568
Bse3DI GCAATG 1 cut(s) 401
BseBI CCWGG 1 cut(s) 458
BseDI CCNNGG 2 cut(s) 456, 600
BseGI GGATG 1 cut(s) 997
BseMI GCAATG 1 cut(s) 401
BseMII CTCAG 2 cut(s) 342, 605
BseNI ACTGG 1 cut(s) 568
BseRI GAGGAG 2 cut(s) 211, 782
BseXI GCAGC 5 cut(s) 403, 583, 810, 859, 972
Bsh1236I CGCG 3 cut(s) 308, 794, 800
Bsh1285I CGRYCG 1 cut(s) 798
BshFI GGCC 3 cut(s) 14, 490, 667
BsiEI CGRYCG 1 cut(s) 798
BsiSI CCGG 1 cut(s) 97
BslFI GGGAC 1 cut(s) 934
BsmAI GTCTC 1 cut(s) 1026
BsmFI GGGAC 1 cut(s) 934
BsmI GAATGC 1 cut(s) 654
BsnI GGCC 3 cut(s) 14, 490, 667
Bsp119I TTCGAA 1 cut(s) 787
Bsp1407I TGTACA 1 cut(s) 85
Bsp143I GATC 7 cut(s) 34, 157, 172, 208, 512, 795, 912
BspANI GGCC 3 cut(s) 14, 490, 667
BspCNI CTCAG 2 cut(s) 343, 604
BspFNI CGCG 3 cut(s) 308, 794, 800
BspPI GGATC 2 cut(s) 167, 203
BspQI GCTCTTC 1 cut(s) 529
BspT104I TTCGAA 1 cut(s) 787
BsrDI GCAATG 1 cut(s) 401
BsrGI TGTACA 1 cut(s) 85
BsrI ACTGG 1 cut(s) 568
BssECI CCNNGG 2 cut(s) 456, 600
BssMI GATC 7 cut(s) 34, 157, 172, 208, 512, 795, 912
BssNI GRCGYC 1 cut(s) 24
BssT1I CCWWGG 1 cut(s) 600
Bst2UI CCWGG 1 cut(s) 458
Bst4CI ACNGT 1 cut(s) 250
Bst6I CTCTTC 8 cut(s) 183, 456, 502, 529, 538, 630, 771, 899
BstACI GRCGYC 1 cut(s) 24
BstAUI TGTACA 1 cut(s) 85
BstBAI YACGTR 1 cut(s) 740
BstBI TTCGAA 1 cut(s) 787
BstC8I GCNNGC 5 cut(s) 12, 124, 879, 883, 1060
BstDEI CTNAG 3 cut(s) 351, 591, 872
BstF5I GGATG 1 cut(s) 997
BstFNI CGCG 3 cut(s) 308, 794, 800
BstH2I RGCGCY 1 cut(s) 839
BstHHI GCGC 1 cut(s) 838
BstKTI GATC 7 cut(s) 37, 160, 175, 211, 515, 798, 915
BstMAI GTCTC 1 cut(s) 1026
BstMBI GATC 7 cut(s) 34, 157, 172, 208, 512, 795, 912
BstMCI CGRYCG 1 cut(s) 798
BstMWI GCNNNNNNNGC 2 cut(s) 844, 874
BstNI CCWGG 1 cut(s) 458
BstNSI RCATGY 4 cut(s) 126, 583, 885, 1062
BstSCI CCNGG 1 cut(s) 456
BstUI CGCG 3 cut(s) 308, 794, 800
BstV1I GCAGC 5 cut(s) 403, 583, 810, 859, 972
BstV2I GAAGAC 2 cut(s) 51, 1011
BsuI GTATCC 1 cut(s) 984
BsuRI GGCC 3 cut(s) 14, 490, 667
BtsCI GGATG 1 cut(s) 997
Cac8I GCNNGC 5 cut(s) 12, 124, 879, 883, 1060
CfoI GCGC 1 cut(s) 838
CseI GACGC 1 cut(s) 857
Csp6I GTAC 5 cut(s) 19, 86, 152, 571, 737
CviAII CATG 7 cut(s) 89, 123, 445, 580, 882, 916, 1059
CviQI GTAC 5 cut(s) 19, 86, 152, 571, 737
DdeI CTNAG 3 cut(s) 351, 591, 872
DpnI GATC 7 cut(s) 36, 159, 174, 210, 514, 797, 914
DpnII GATC 7 cut(s) 34, 157, 172, 208, 512, 795, 912
EaeI YGGCCR 1 cut(s) 12
Eam1104I CTCTTC 8 cut(s) 183, 456, 502, 529, 538, 630, 771, 899
EarI CTCTTC 8 cut(s) 183, 456, 502, 529, 538, 630, 771, 899
Eco130I CCWWGG 1 cut(s) 600
Eco57I CTGAAG 1 cut(s) 650
EcoRII CCWGG 1 cut(s) 456
EcoT14I CCWWGG 1 cut(s) 600
EcoT22I ATGCAT 3 cut(s) 124, 1060, 1064
ErhI CCWWGG 1 cut(s) 600
FaeI CATG 7 cut(s) 92, 126, 448, 583, 885, 919, 1062
FalI AAGNNNNNCTT 2 cut(s) 768, 800
FaqI GGGAC 1 cut(s) 934
FatI CATG 7 cut(s) 88, 122, 444, 579, 881, 915, 1058
Fnu4HI GCNGC 5 cut(s) 392, 597, 824, 848, 961
FokI GGATG 1 cut(s) 1004
Fsp4HI GCNGC 5 cut(s) 392, 597, 824, 848, 961
GlaI GCGC 1 cut(s) 837
GluI GCNGC 5 cut(s) 392, 597, 824, 848, 961
GsuI CTGGAG 2 cut(s) 827, 848
HaeII RGCGCY 1 cut(s) 839
HaeIII GGCC 3 cut(s) 14, 490, 667
HapII CCGG 1 cut(s) 97
HgaI GACGC 1 cut(s) 857
HhaI GCGC 1 cut(s) 838
Hin1I GRCGYC 1 cut(s) 24
Hin1II CATG 7 cut(s) 92, 126, 448, 583, 885, 919, 1062
Hin6I GCGC 1 cut(s) 836
HinP1I GCGC 1 cut(s) 836
HincII GTYRAC 2 cut(s) 348, 681
HindII GTYRAC 2 cut(s) 348, 681
HindIII AAGCTT 1 cut(s) 782
HinfI GANTC 2 cut(s) 452, 1034
HpaI GTTAAC 1 cut(s) 348
HpaII CCGG 1 cut(s) 97
HphI GGTGA 1 cut(s) 53
Hpy166II GTNNAC 2 cut(s) 348, 681
Hpy188I TCNGA 7 cut(s) 245, 296, 411, 630, 675, 704, 1033
Hpy188III TCNNGA 4 cut(s) 170, 552, 644, 746
Hpy8I GTNNAC 2 cut(s) 348, 681
Hpy99I CGWCG 3 cut(s) 26, 29, 663
HpyAV CCTTC 5 cut(s) 81, 192, 698, 755, 919
HpyCH4III ACNGT 1 cut(s) 250
HpyCH4IV ACGT 3 cut(s) 24, 612, 739
HpyCH4V TGCA 7 cut(s) 122, 256, 417, 847, 960, 1058, 1062
HpyF10VI GCNNNNNNNGC 2 cut(s) 844, 874
HpyF3I CTNAG 3 cut(s) 351, 591, 872
HpySE526I ACGT 3 cut(s) 24, 612, 739
Hsp92I GRCGYC 1 cut(s) 24
Hsp92II CATG 7 cut(s) 92, 126, 448, 583, 885, 919, 1062
HspAI GCGC 1 cut(s) 836
KspAI GTTAAC 1 cut(s) 348
Kzo9I GATC 7 cut(s) 34, 157, 172, 208, 512, 795, 912
LguI GCTCTTC 1 cut(s) 529
Lsp1109I GCAGC 5 cut(s) 403, 583, 810, 859, 972
LweI GCATC 2 cut(s) 982, 1071
MaeII ACGT 3 cut(s) 24, 612, 739
MaeIII GTNAC 1 cut(s) 725
MalI GATC 7 cut(s) 36, 159, 174, 210, 514, 797, 914
MboI GATC 7 cut(s) 34, 157, 172, 208, 512, 795, 912
MlsI TGGCCA 1 cut(s) 14
MluCI AATT 6 cut(s) 103, 134, 285, 322, 377, 895
MluI ACGCGT 1 cut(s) 306
MluNI TGGCCA 1 cut(s) 14
MlyI GAGTC 1 cut(s) 1028
MmeI TCCRAC 1 cut(s) 1001
Mox20I TGGCCA 1 cut(s) 14
Mph1103I ATGCAT 3 cut(s) 124, 1060, 1064
MscI TGGCCA 1 cut(s) 14
MseI TTAA 6 cut(s) 101, 321, 347, 357, 717, 1075
Msp20I TGGCCA 1 cut(s) 14
MspI CCGG 1 cut(s) 97
MspR9I CCNGG 1 cut(s) 458
Mva1269I GAATGC 1 cut(s) 654
MvaI CCWGG 1 cut(s) 458
MvnI CGCG 3 cut(s) 308, 794, 800
MwoI GCNNNNNNNGC 2 cut(s) 844, 874
NdeII GATC 7 cut(s) 34, 157, 172, 208, 512, 795, 912
NlaIII CATG 7 cut(s) 92, 126, 448, 583, 885, 919, 1062
NsiI ATGCAT 3 cut(s) 124, 1060, 1064
NspI RCATGY 4 cut(s) 126, 583, 885, 1062
NspV TTCGAA 1 cut(s) 787
PaeI GCATGC 3 cut(s) 126, 885, 1062
PciSI GCTCTTC 1 cut(s) 529
PcsI WCGNNNNNNNCGW 1 cut(s) 1005
PctI GAATGC 1 cut(s) 654
PfeI GAWTC 1 cut(s) 452
PflFI GACNNNGTC 1 cut(s) 26
PkrI GCNGC 5 cut(s) 393, 598, 825, 849, 962
Ple19I CGATCG 1 cut(s) 798
PleI GAGTC 1 cut(s) 1028
PpsI GAGTC 1 cut(s) 1028
Ppu21I YACGTR 1 cut(s) 740
PshBI ATTAAT 1 cut(s) 357
Psp6I CCWGG 1 cut(s) 456
PspGI CCWGG 1 cut(s) 456
PsyI GACNNNGTC 1 cut(s) 26
PvuI CGATCG 1 cut(s) 798
RgaI GCGATCGC 1 cut(s) 798
RsaI GTAC 5 cut(s) 20, 87, 153, 572, 738
RsaNI GTAC 5 cut(s) 19, 86, 152, 571, 737
SapI GCTCTTC 1 cut(s) 529
SaqAI TTAA 6 cut(s) 101, 321, 347, 357, 717, 1075
SatI GCNGC 5 cut(s) 392, 597, 824, 848, 961
Sau3AI GATC 7 cut(s) 34, 157, 172, 208, 512, 795, 912
ScaI AGTACT 1 cut(s) 572
SchI GAGTC 1 cut(s) 1028
ScrFI CCNGG 1 cut(s) 458
SfaAI GCGATCGC 1 cut(s) 798
SfaNI GCATC 2 cut(s) 982, 1071
SfuI TTCGAA 1 cut(s) 787
SgfI GCGATCGC 1 cut(s) 798
SmlI CTYRAG 2 cut(s) 188, 851
SmoI CTYRAG 2 cut(s) 188, 851
SphI GCATGC 3 cut(s) 126, 885, 1062
Sse9I AATT 6 cut(s) 103, 134, 285, 322, 377, 895
SspI AATATT 1 cut(s) 205
StyD4I CCNGG 1 cut(s) 456
StyI CCWWGG 1 cut(s) 600
TaaI ACNGT 1 cut(s) 250
TaiI ACGT 3 cut(s) 27, 615, 742
TaqI TCGA 4 cut(s) 643, 787, 986, 1046
TasI AATT 6 cut(s) 103, 134, 285, 322, 377, 895
TatI WGTACW 3 cut(s) 85, 151, 570
TfiI GAWTC 1 cut(s) 452
Tru1I TTAA 6 cut(s) 101, 321, 347, 357, 717, 1075
Tru9I TTAA 6 cut(s) 101, 321, 347, 357, 717, 1075
TseI GCWGC 5 cut(s) 391, 596, 823, 847, 960
TspDTI ATGAA 4 cut(s) 63, 297, 441, 461
TspGWI ACGGA 1 cut(s) 455
Tth111I GACNNNGTC 1 cut(s) 26
VspI ATTAAT 1 cut(s) 357
XapI RAATTY 1 cut(s) 285
XceI RCATGY 4 cut(s) 126, 583, 885, 1062
ZraI GACGTC 1 cut(s) 25
ZrmI AGTACT 1 cut(s) 572
Zsp2I ATGCAT 3 cut(s) 124, 1060, 1064
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.