Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr14

Orthogroup ID

OG0005635

Physical Location & Seq

Genomic Coordinates Reverse (-)

20552033 .. 20554286

Gene Structure 2254 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 942 bp

ATGGGCTCAGTTGGGTTTTCTCTCCACTCACCGTTCAGCTCTCTGCTCCTCTGCTCTGCTCCTCATTCCGCTCGAAACCCCAGAAAACCAAAACCCTTTTCAAAATTCACCCCAAAAATGGCTCTGGCCACCCCTACCACTTTTGGGTTCAGCAGTCTGCTCGAGACGTTCACGTTGAACGTGCAGAGAGCAGAGAATCGGCCGCTTAACGTGCCGCTGACTGCGCCGTTCACGATTGCGACTTCGCGGCTGGAGAGGGTGGAGAATGTGGCGGTCCGGGTTGAGTTGAGTAACGGGTGCGTGGGGTGGGGTGAAACTCCGATTTTGCCCTTTGTAACTGCAGAGGATCAGCATACTGCCATGGTCAAGGCTAAGGAAGCTTGCGAGTTCTTGCTTCGAAGTCCGGCGATGACGTTGGGTTCGGTTTTGGGGGAGATTGGTGGAATTCTTCCTGGACATGAGTTTGCTTCTGTTAGGGCAGGAGTTGAGATGGCGTTAATTGATGCAGTTTCTATGAGTATCGGTGTCCCTTTGTGGAGACTGTTTGGTGGAGCTTCAAACAGCATAACTACTGATATAACAATTCCGATTGTTTCTGCAGCAGAAGCTGCTACATTGGCTTCAAAGTACCGTGAAGAAGGATTTAAAACTTTAAAGCTTAAGGTGGGAAAGAATTTGAATTCAGACATAGAAGTCCTTCAAGCTATACGTGTTGTTCACCCCGATTGCTATTTTATCTTGGATGCTAACGAAGGATATAACTCCAACGAAGCTATCCAAGTTCTTGATAAATTACATGAGGTGGGAGTGAGTCCTCTTCTCTTCGAACAACCGGTCCATAGAGATGATTGGAAAGGCCTCGGAAATGTTTACCGCGTTGCGAAAGACAAATATGGAGTATCAGTTGCAGCTGATGAGAGCTGTCGAAGTTTAGCTGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000287 GO:0003674 GO:0003824 GO:0005488 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016853 GO:0016854 GO:0016855 GO:0034641 GO:0043167 GO:0043169 GO:0043603 GO:0044237 GO:0046872 GO:0071704 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MR_MLE_C MR_MLE_N

Protein Analysis

314

Amino Acids

33.84

Weight (kDa)

6.19

Isoelectric Point (pI)

33.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MR_MLE_N	PF02746	71 - 183	5.7e-07	Mandelate racemase / muconate lactonizing enzyme, N-terminal domain
MR_MLE_C	PF13378	204 - 313	2.5e-18	Enolase C-terminal domain-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005635)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G18270
fragaria_vesca	FvH4_5g16670 FvH4_5g16670 FvH4_5g16670 FvH4_5g16670 FvH4_5g16670 FvH4_5g16670
malus_domestica	MD06G1220000.v1.1 MD14G1229600.v1.1
prunus_persica	Prupe.5G224400_v2.0.a1 Prupe.5G224400_v2.0.a1
pyrus_communis	pycom14g19100 pycom14g19110
rosa_chinensis	RchiOBHm_Chr7g0179451
rosa_laevigata	RLG00000005355
rosa_multiflora	Rmu_co8470985.1_g000001
rosa_roxburghii	Rroxscaffold_3G00274010
rosa_rugosa	Rorug06G0424300 Rorug06G0424400 Rorug06G0424500 Rorug06G0424600
rosa_samantha	Rh2AG318700 Rh6AG144500 Rh7BG023500
rosa_wichuraiana	Rw4G009460 Rw4G033460 Rw5G048300 Rw7G001940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	692
AccBSI	CCGCTC	1 cut(s)	71
AccII	CGCG	2 cut(s)	247, 876
AciI	CCGC	6 cut(s)	69, 203, 215, 247, 272, 874
AclWI	GGATC	1 cut(s)	354
AcoI	YGGCCR	2 cut(s)	126, 200
AcsI	RAATTY	4 cut(s)	104, 444, 673, 679
AfaI	GTAC	1 cut(s)	629
AfiI	CCNNNNNNNGG	2 cut(s)	118, 144
AflII	CTTAAG	1 cut(s)	659
AflIII	ACRYGT	1 cut(s)	709
AgeI	ACCGGT	1 cut(s)	832
AgsI	TTSAA	6 cut(s)	102, 178, 558, 624, 679, 701
AjnI	CCWGG	1 cut(s)	451
AjuI	GAANNNNNNNTTGG	2 cut(s)	82, 114
AloI	GAACNNNNNNTCC	2 cut(s)	819, 851
Alw26I	GTCTC	2 cut(s)	158, 532
AlwI	GGATC	1 cut(s)	354
AlwNI	CAGNNNCTG	1 cut(s)	608
Ama87I	CYCGRG	1 cut(s)	161
AoxI	GGCC	3 cut(s)	126, 200, 856
ApeKI	GCWGC	3 cut(s)	599, 608, 908
ApoI	RAATTY	4 cut(s)	104, 444, 673, 679
ArsI	GACNNNNNNTTYG	4 cut(s)	403, 435, 819, 851
AsiGI	ACCGGT	1 cut(s)	832
Asp700I	GAANNNNTTC	1 cut(s)	696
AspLEI	GCGC	1 cut(s)	226
AspS9I	GGNCC	2 cut(s)	274, 835
AsuC2I	CCSGG	1 cut(s)	278
AsuHPI	GGTGA	4 cut(s)	21, 100, 323, 710
AsuII	TTCGAA	2 cut(s)	397, 825
AvaI	CYCGRG	1 cut(s)	161
AvaII	GGWCC	2 cut(s)	274, 835
BalI	TGGCCA	1 cut(s)	128
BanII	GRGCYC	1 cut(s)	8
BbvI	GCAGC	3 cut(s)	595, 611, 920
BccI	CCATC	1 cut(s)	484
BceAI	ACGGC	1 cut(s)	211
BcgI	CGANNNNNNTGC	2 cut(s)	142, 176
BciT130I	CCWGG	1 cut(s)	453
BcnI	CCSGG	1 cut(s)	278
BcoDI	GTCTC	2 cut(s)	158, 532
BfmI	CTRYAG	2 cut(s)	339, 597
BfrI	CTTAAG	1 cut(s)	659
BisI	GCNGC	6 cut(s)	203, 215, 248, 600, 609, 909
BlsI	GCNGC	6 cut(s)	204, 216, 249, 601, 610, 910
Bme1390I	CCNGG	2 cut(s)	278, 453
Bme18I	GGWCC	2 cut(s)	274, 835
BmeT110I	CYCGRG	1 cut(s)	161
BmgT120I	GGNCC	2 cut(s)	274, 835
BmrFI	CCNGG	2 cut(s)	278, 453
BmsI	GCATC	2 cut(s)	493, 733
BpmI	CTGGAG	1 cut(s)	272
Bpu10I	CCTNAGC	1 cut(s)	372
Bpu14I	TTCGAA	2 cut(s)	397, 825
BpuMI	CCSGG	1 cut(s)	278
BsaAI	YACGTR	1 cut(s)	710
BsaJI	CCNNGG	2 cut(s)	360, 859
BsaWI	WCCGGW	1 cut(s)	832
BsaXI	ACNNNNNCTCC	2 cut(s)	888, 918
Bsc4I	CCNNNNNNNGG	2 cut(s)	118, 144
Bse118I	RCCGGY	1 cut(s)	832
BseBI	CCWGG	1 cut(s)	453
BseDI	CCNNGG	2 cut(s)	360, 859
BseGI	GGATG	1 cut(s)	748
BseLI	CCNNNNNNNGG	2 cut(s)	118, 144
BseMII	CTCAG	1 cut(s)	21
BseRI	GAGGAG	2 cut(s)	38, 51
BseX3I	CGGCCG	1 cut(s)	200
BseXI	GCAGC	3 cut(s)	595, 611, 920
BsgI	GTGCAG	1 cut(s)	203
Bsh1236I	CGCG	2 cut(s)	247, 876
Bsh1285I	CGRYCG	1 cut(s)	203
BshFI	GGCC	3 cut(s)	128, 202, 858
BshTI	ACCGGT	1 cut(s)	832
BsiEI	CGRYCG	1 cut(s)	203
BsiHKCI	CYCGRG	1 cut(s)	161
BsiSI	CCGG	3 cut(s)	277, 404, 833
BslFI	GGGAC	1 cut(s)	512
BslI	CCNNNNNNNGG	2 cut(s)	118, 144
BsmAI	GTCTC	2 cut(s)	158, 532
BsmBI	CGTCTC	1 cut(s)	158
BsmFI	GGGAC	1 cut(s)	512
BsnI	GGCC	3 cut(s)	128, 202, 858
BsoBI	CYCGRG	1 cut(s)	161
Bsp119I	TTCGAA	2 cut(s)	397, 825
Bsp1286I	GDGCHC	1 cut(s)	8
Bsp143I	GATC	1 cut(s)	346
Bsp19I	CCATGG	1 cut(s)	360
BspACI	CCGC	6 cut(s)	69, 203, 215, 247, 272, 874
BspANI	GGCC	3 cut(s)	128, 202, 858
BspCNI	CTCAG	1 cut(s)	20
BspFNI	CGCG	2 cut(s)	247, 876
BspMAI	CTGCAG	2 cut(s)	343, 601
BspPI	GGATC	1 cut(s)	354
BspT104I	TTCGAA	2 cut(s)	397, 825
BspTI	CTTAAG	1 cut(s)	659
BsrBI	CCGCTC	1 cut(s)	71
BsrFI	RCCGGY	1 cut(s)	832
BssAI	RCCGGY	1 cut(s)	832
BssECI	CCNNGG	2 cut(s)	360, 859
BssMI	GATC	1 cut(s)	346
BssT1I	CCWWGG	1 cut(s)	360
Bst2UI	CCWGG	1 cut(s)	453
Bst4CI	ACNGT	3 cut(s)	33, 543, 632
Bst6I	CTCTTC	2 cut(s)	822, 827
BstAFI	CTTAAG	1 cut(s)	659
BstAPI	GCANNNNNTGC	1 cut(s)	608
BstBAI	YACGTR	1 cut(s)	710
BstBI	TTCGAA	2 cut(s)	397, 825
BstC8I	GCNNGC	1 cut(s)	382
BstDEI	CTNAG	2 cut(s)	7, 372
BstDSI	CCRYGG	1 cut(s)	360
BstF5I	GGATG	1 cut(s)	748
BstFNI	CGCG	2 cut(s)	247, 876
BstHHI	GCGC	1 cut(s)	226
BstKTI	GATC	1 cut(s)	349
BstMAI	GTCTC	2 cut(s)	158, 532
BstMBI	GATC	1 cut(s)	346
BstMCI	CGRYCG	1 cut(s)	203
BstMWI	GCNNNNNNNGC	6 cut(s)	211, 223, 377, 605, 608, 617
BstNI	CCWGG	1 cut(s)	453
BstSCI	CCNGG	2 cut(s)	276, 451
BstSFI	CTRYAG	2 cut(s)	339, 597
BstUI	CGCG	2 cut(s)	247, 876
BstV1I	GCAGC	3 cut(s)	595, 611, 920
BstZI	CGGCCG	1 cut(s)	200
BsuRI	GGCC	3 cut(s)	128, 202, 858
BtgI	CCRYGG	1 cut(s)	360
BtgZI	GCGATG	1 cut(s)	422
BtsCI	GGATG	1 cut(s)	748
Cac8I	GCNNGC	1 cut(s)	382
CaiI	CAGNNNCTG	1 cut(s)	608
CfoI	GCGC	1 cut(s)	226
Cfr10I	RCCGGY	1 cut(s)	832
Cfr13I	GGNCC	2 cut(s)	274, 835
CpoI	CGGWCCG	1 cut(s)	274
Csp6I	GTAC	1 cut(s)	628
CspAI	ACCGGT	1 cut(s)	832
CspI	CGGWCCG	1 cut(s)	274
CviAII	CATG	3 cut(s)	361, 458, 797
CviQI	GTAC	1 cut(s)	628
DdeI	CTNAG	2 cut(s)	7, 372
DpnI	GATC	1 cut(s)	348
DpnII	GATC	1 cut(s)	346
DraI	TTTAAA	2 cut(s)	646, 654
DrdI	GACNNNNNNGTC	1 cut(s)	692
DseDI	GACNNNNNNGTC	1 cut(s)	692
EaeI	YGGCCR	2 cut(s)	126, 200
EagI	CGGCCG	1 cut(s)	200
Eam1104I	CTCTTC	2 cut(s)	822, 827
EarI	CTCTTC	2 cut(s)	822, 827
EclXI	CGGCCG	1 cut(s)	200
Eco130I	CCWWGG	1 cut(s)	360
Eco147I	AGGCCT	1 cut(s)	858
Eco24I	GRGCYC	1 cut(s)	8
Eco47I	GGWCC	2 cut(s)	274, 835
Eco52I	CGGCCG	1 cut(s)	200
Eco88I	CYCGRG	1 cut(s)	161
EcoRI	GAATTC	2 cut(s)	444, 679
EcoRII	CCWGG	1 cut(s)	451
EcoT14I	CCWWGG	1 cut(s)	360
EcoT38I	GRGCYC	1 cut(s)	8
ErhI	CCWWGG	1 cut(s)	360
Esp3I	CGTCTC	1 cut(s)	158
FaeI	CATG	3 cut(s)	364, 461, 800
FaqI	GGGAC	1 cut(s)	512
FatI	CATG	3 cut(s)	360, 457, 796
Fnu4HI	GCNGC	6 cut(s)	203, 215, 248, 600, 609, 909
FokI	GGATG	1 cut(s)	755
FriOI	GRGCYC	1 cut(s)	8
Fsp4HI	GCNGC	6 cut(s)	203, 215, 248, 600, 609, 909
GlaI	GCGC	1 cut(s)	225
GluI	GCNGC	6 cut(s)	203, 215, 248, 600, 609, 909
GsuI	CTGGAG	1 cut(s)	272
HaeIII	GGCC	3 cut(s)	128, 202, 858
HapII	CCGG	3 cut(s)	277, 404, 833
HhaI	GCGC	1 cut(s)	226
Hin1II	CATG	3 cut(s)	364, 461, 800
Hin6I	GCGC	1 cut(s)	224
HinP1I	GCGC	1 cut(s)	224
HindIII	AAGCTT	2 cut(s)	378, 656
HinfI	GANTC	2 cut(s)	196, 811
HpaII	CCGG	3 cut(s)	277, 404, 833
HphI	GGTGA	4 cut(s)	21, 100, 323, 710
Hpy166II	GTNNAC	4 cut(s)	171, 231, 718, 871
Hpy188I	TCNGA	4 cut(s)	321, 588, 685, 863
Hpy188III	TCNNGA	3 cut(s)	163, 232, 785
Hpy8I	GTNNAC	4 cut(s)	171, 231, 718, 871
HpyAV	CCTTC	3 cut(s)	632, 707, 746
HpyCH4III	ACNGT	3 cut(s)	33, 543, 632
HpyCH4IV	ACGT	6 cut(s)	167, 173, 180, 210, 413, 709
HpyCH4V	TGCA	5 cut(s)	184, 341, 506, 599, 908
HpyF10VI	GCNNNNNNNGC	6 cut(s)	211, 223, 377, 605, 608, 617
HpyF3I	CTNAG	2 cut(s)	7, 372
HpySE526I	ACGT	6 cut(s)	167, 173, 180, 210, 413, 709
Hsp92II	CATG	3 cut(s)	364, 461, 800
HspAI	GCGC	1 cut(s)	224
Kzo9I	GATC	1 cut(s)	346
LmnI	GCTCC	3 cut(s)	51, 64, 551
LpnPI	CCDG	9 cut(s)	94, 110, 236, 290, 417, 438, 465, 465, 846
Lsp1109I	GCAGC	3 cut(s)	595, 611, 920
LweI	GCATC	2 cut(s)	493, 733
MaeII	ACGT	6 cut(s)	167, 173, 180, 210, 413, 709
MaeIII	GTNAC	2 cut(s)	290, 334
MalI	GATC	1 cut(s)	348
MbiI	CCGCTC	1 cut(s)	71
MboI	GATC	1 cut(s)	346
MboII	GAAGA	4 cut(s)	440, 647, 809, 814
MhlI	GDGCHC	1 cut(s)	8
MlsI	TGGCCA	1 cut(s)	128
MluCI	AATT	7 cut(s)	104, 444, 498, 582, 673, 679, 791
MluNI	TGGCCA	1 cut(s)	128
MlyI	GAGTC	1 cut(s)	820
MmeI	TCCRAC	1 cut(s)	789
MnlI	CCTC	7 cut(s)	59, 72, 249, 337, 793, 825, 869
Mox20I	TGGCCA	1 cut(s)	128
MroXI	GAANNNNTTC	1 cut(s)	696
MscI	TGGCCA	1 cut(s)	128
MseI	TTAA	6 cut(s)	207, 497, 645, 653, 660, 940
MslI	CAYNNNNRTG	1 cut(s)	843
Msp20I	TGGCCA	1 cut(s)	128
MspA1I	CMGCKG	2 cut(s)	217, 911
MspCI	CTTAAG	1 cut(s)	659
MspI	CCGG	3 cut(s)	277, 404, 833
MspR9I	CCNGG	2 cut(s)	278, 453
MvaI	CCWGG	1 cut(s)	453
MvnI	CGCG	2 cut(s)	247, 876
MwoI	GCNNNNNNNGC	6 cut(s)	211, 223, 377, 605, 608, 617
NciI	CCSGG	1 cut(s)	278
NcoI	CCATGG	1 cut(s)	360
NdeII	GATC	1 cut(s)	346
NlaIII	CATG	3 cut(s)	364, 461, 800
NspV	TTCGAA	2 cut(s)	397, 825
PaeR7I	CTCGAG	1 cut(s)	161
PceI	AGGCCT	1 cut(s)	858
PdmI	GAANNNNTTC	1 cut(s)	696
PfeI	GAWTC	1 cut(s)	196
PfoI	TCCNGGA	1 cut(s)	451
PinAI	ACCGGT	1 cut(s)	832
PkrI	GCNGC	6 cut(s)	204, 216, 249, 601, 610, 910
PleI	GAGTC	1 cut(s)	819
PpsI	GAGTC	1 cut(s)	819
Ppu21I	YACGTR	1 cut(s)	710
Psp6I	CCWGG	1 cut(s)	451
PspGI	CCWGG	1 cut(s)	451
PspPI	GGNCC	2 cut(s)	274, 835
PstI	CTGCAG	2 cut(s)	343, 601
PstNI	CAGNNNCTG	1 cut(s)	608
PvuII	CAGCTG	1 cut(s)	911
RsaI	GTAC	1 cut(s)	629
RsaNI	GTAC	1 cut(s)	628
RseI	CAYNNNNRTG	1 cut(s)	843
Rsr2I	CGGWCCG	1 cut(s)	274
RsrII	CGGWCCG	1 cut(s)	274
SaqAI	TTAA	6 cut(s)	207, 497, 645, 653, 660, 940
SatI	GCNGC	6 cut(s)	203, 215, 248, 600, 609, 909
Sau3AI	GATC	1 cut(s)	346
Sau96I	GGNCC	2 cut(s)	274, 835
SchI	GAGTC	1 cut(s)	820
ScrFI	CCNGG	2 cut(s)	278, 453
SduI	GDGCHC	1 cut(s)	8
SfaNI	GCATC	2 cut(s)	493, 733
SfcI	CTRYAG	2 cut(s)	339, 597
Sfr274I	CTCGAG	1 cut(s)	161
SfuI	TTCGAA	2 cut(s)	397, 825
SinI	GGWCC	2 cut(s)	274, 835
SlaI	CTCGAG	1 cut(s)	161
SmiMI	CAYNNNNRTG	1 cut(s)	843
SmlI	CTYRAG	2 cut(s)	161, 659
SmoI	CTYRAG	2 cut(s)	161, 659
Sse9I	AATT	7 cut(s)	104, 444, 498, 582, 673, 679, 791
SseBI	AGGCCT	1 cut(s)	858
SsiI	CCGC	6 cut(s)	69, 203, 215, 247, 272, 874
StuI	AGGCCT	1 cut(s)	858
StyD4I	CCNGG	2 cut(s)	276, 451
StyI	CCWWGG	1 cut(s)	360
TaaI	ACNGT	3 cut(s)	33, 543, 632
TaiI	ACGT	6 cut(s)	170, 176, 183, 213, 416, 712
TaqI	TCGA	5 cut(s)	73, 162, 397, 825, 925
TasI	AATT	7 cut(s)	104, 444, 498, 582, 673, 679, 791
TauI	GCSGC	3 cut(s)	205, 217, 250
TfiI	GAWTC	1 cut(s)	196
Tru1I	TTAA	6 cut(s)	207, 497, 645, 653, 660, 940
Tru9I	TTAA	6 cut(s)	207, 497, 645, 653, 660, 940
TseI	GCWGC	3 cut(s)	599, 608, 908
Vha464I	CTTAAG	1 cut(s)	659
VpaK11BI	GGWCC	2 cut(s)	274, 835
XapI	RAATTY	4 cut(s)	104, 444, 673, 679
XhoI	CTCGAG	1 cut(s)	161
XmnI	GAANNNNTTC	1 cut(s)	696

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.