pycom14g20470
MYB Family

transcription, DNA-templated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0008575
Physical Location & Seq
Forward (+)
21530476 .. 21532708
2233 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1326 bp
ATGGCAGTTGAGGATCGGATTGAAGACACAGGTTCAAACGCCTCTGTTGCGGTTGGAAATTGCAGTTCAAACGGTGATGCGCAATCTAATGCGGAGATTTGTTCATTTGGCAATGACCATGCACCCAAGGTTAGGAAACCGTATACGATTACAAAACAAAGAGAAAAATGGACAGAGGAGGAGCATCAGAAGTTCCTTGAAGCCTTGAAATTGTACGGCCGAGGTTGGCGTCAAATAGAAGAGTATGTAGGCACCAAAACTGCAGTTCAAATTCGAAGTCATGCCCAAAAGTTCTTCTCTAAGCTATCTAAGGAATCATGTGGACCTAGCGAAGGCATCATCAGACTGATTGAGATTCCTCCTCCCCGGCCTAAGCGGAGGCCTGTGCATCCTTATCCACGTAAATCAGTGGATTATCTCAATGGATCACCAGAAAGGTCACCATCCCCACAATTTTCGGCTCAAGGGAAAGACCAACAATCTCCCACTTCAGTACTATCAGCACATGATTCGGATGTACTGGGGTCTGCTGCTTTTGACCAGCATAACAGATCTCCACCCCCGACTTCATGTACCACTGACATGCACTCTACGAGCTTATGCCCCTTTGGAAAAGAGAGTGATTACGTGGTGTCCAACTCATCCGCTGACGAAGTGAAAGGGTCCTCGCCATTGGTCCAATTGGAAAAATTTCCATCCGTGAATTACAGATCCGGTTCCAAGCACACTGTGAGTACTGAAGAAGATGCCGGCAATATAAGAGCTTCTGTGAGCATTAAGCTCTTTGGAAGGACAGTTTTGGTACCAGACTCGGAGAAACAACCTCCACCTGGTGCAGAGGATTCTAAGATGTTGACATCAAAGGTGGAAGAAGACAAATGTGAGAGGGAGAATGATAAGCTTGTTCAATTATCAAGATCAGATCAATTAGATACAAGTTTAACACTCGGTGGGATTGTTGGCAACGTGGTTTCATCGGCATGTGGTGCTACAACAGAATGGGAACACCAAAAACATAGCCCTAATCCTGAAGCTCCTCTGCCATGGTGGTCTATGTATCATGGTTTTATGGACCCTCACGTCAGATCGTGTAACCAAAACTCAGCTAAAATTACGGGAAGATCCTTTCATAGTGCAGTGGAAAGTTTAGGTGAGAGGCGCATAGAGGCTGTCGATTCATTACTCGAAGAAACTTGTTCGGTGCCCTGCAGTTCACGGAAGGGGTTTGCAGCGTACAAAAGATGTTTAGCAGATGTAAATAAGAACTCATCTGCCATAGTTTATGAAGACATGAGGGACAGGCGAAGGGCTCGTGTCTGCTCATAG

Protein Analysis

442

Amino Acids

48.68

Weight (kDa)

6.9

Isoelectric Point (pI)

62.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 54 - 97 2.6e-13 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1079
Acc16I TGCGCA 1 cut(s) 81
Acc65I GGTACC 1 cut(s) 802
AccB1I GGYRCC 3 cut(s) 251, 802, 1201
AccI GTMKAC 1 cut(s) 143
AciI CCGC 4 cut(s) 50, 92, 376, 645
AclWI GGATC 4 cut(s) 21, 433, 705, 1116
AcoI YGGCCR 1 cut(s) 217
AcsI RAATTY 2 cut(s) 270, 689
AcuI CTGAAG 3 cut(s) 474, 759, 1050
AcyI GRCGYC 1 cut(s) 229
AdeI CACNNNGTG 3 cut(s) 730, 833, 950
AfaI GTAC 7 cut(s) 215, 495, 519, 574, 736, 804, 1235
AfiI CCNNNNNNNGG 3 cut(s) 132, 332, 830
AgsI TTSAA 7 cut(s) 23, 36, 69, 200, 208, 269, 908
AjiI CACGTC 1 cut(s) 1081
AjnI CCWGG 1 cut(s) 829
AluBI AGCT 7 cut(s) 304, 597, 764, 781, 901, 1034, 1106
AluI AGCT 7 cut(s) 304, 597, 764, 781, 901, 1034, 1106
AlwI GGATC 4 cut(s) 21, 433, 705, 1116
AoxI GGCC 3 cut(s) 217, 368, 380
ApeKI GCWGC 2 cut(s) 530, 1229
ApoI RAATTY 2 cut(s) 270, 689
ArsI GACNNNNNNTTYG 2 cut(s) 262, 294
Asp700I GAANNNNTTC 1 cut(s) 690
Asp718I GGTACC 1 cut(s) 802
AspLEI GCGC 2 cut(s) 82, 1161
AspS9I GGNCC 4 cut(s) 323, 663, 676, 1072
AsuC2I CCSGG 1 cut(s) 367
AsuHPI GGTGA 4 cut(s) 86, 420, 432, 1163
AsuII TTCGAA 1 cut(s) 274
AvaII GGWCC 4 cut(s) 323, 663, 676, 1072
BaeGI GKGCMC 1 cut(s) 1206
BanI GGYRCC 3 cut(s) 251, 802, 1201
BanII GRGCYC 1 cut(s) 1312
BauI CACGAG 1 cut(s) 1311
BbsI GAAGAC 3 cut(s) 30, 879, 1293
BbvI GCAGC 2 cut(s) 517, 1241
BccI CCATC 2 cut(s) 451, 703
BceAI ACGGC 1 cut(s) 232
BcgI CGANNNNNNTGC 2 cut(s) 492, 526
BciT130I CCWGG 1 cut(s) 831
BcnI CCSGG 1 cut(s) 367
BfaI CTAG 1 cut(s) 327
BfmI CTRYAG 2 cut(s) 261, 1207
BglII AGATCT 1 cut(s) 551
BisI GCNGC 2 cut(s) 531, 1230
BlsI GCNGC 2 cut(s) 532, 1231
BmcAI AGTACT 2 cut(s) 495, 736
Bme1390I CCNGG 2 cut(s) 367, 831
Bme18I GGWCC 4 cut(s) 323, 663, 676, 1072
BmgBI CACGTC 1 cut(s) 1081
BmgT120I GGNCC 4 cut(s) 323, 663, 676, 1072
BmiI GGNNCC 6 cut(s) 253, 664, 718, 804, 1074, 1203
BmrFI CCNGG 2 cut(s) 367, 831
BmrI ACTGGG 1 cut(s) 530
BmsI GCATC 5 cut(s) 67, 193, 345, 397, 736
BmuI ACTGGG 1 cut(s) 530
BpiI GAAGAC 3 cut(s) 30, 879, 1293
Bpu10I CCTNAGC 1 cut(s) 372
Bpu14I TTCGAA 1 cut(s) 274
BpuEI CTTGAG 1 cut(s) 447
BpuMI CCSGG 1 cut(s) 367
BsaAI YACGTR 2 cut(s) 401, 628
BsaHI GRCGYC 1 cut(s) 229
BsaJI CCNNGG 4 cut(s) 126, 220, 365, 1043
BsaWI WCCGGW 1 cut(s) 713
Bsc4I CCNNNNNNNGG 3 cut(s) 132, 332, 830
Bse118I RCCGGY 1 cut(s) 749
Bse1I ACTGG 1 cut(s) 525
Bse3DI GCAATG 1 cut(s) 118
BseBI CCWGG 1 cut(s) 831
BseDI CCNNGG 4 cut(s) 126, 220, 365, 1043
BseGI GGATG 5 cut(s) 388, 443, 520, 641, 695
BseLI CCNNNNNNNGG 3 cut(s) 132, 332, 830
BseMI GCAATG 1 cut(s) 118
BseMII CTCAG 1 cut(s) 1116
BseNI ACTGG 1 cut(s) 525
BseRI GAGGAG 4 cut(s) 191, 194, 351, 1026
BseSI GKGCMC 1 cut(s) 1206
BseX3I CGGCCG 1 cut(s) 217
BseXI GCAGC 2 cut(s) 517, 1241
BsgI GTGCAG 2 cut(s) 855, 1155
Bsh1285I CGRYCG 1 cut(s) 220
BshFI GGCC 3 cut(s) 219, 370, 382
BshNI GGYRCC 3 cut(s) 251, 802, 1201
BsiEI CGRYCG 1 cut(s) 220
BsiSI CCGG 3 cut(s) 367, 714, 750
BslFI GGGAC 1 cut(s) 1310
BslI CCNNNNNNNGG 3 cut(s) 132, 332, 830
BsmFI GGGAC 1 cut(s) 1310
BsnI GGCC 3 cut(s) 219, 370, 382
Bsp119I TTCGAA 1 cut(s) 274
Bsp1286I GDGCHC 2 cut(s) 1206, 1312
Bsp143I GATC 8 cut(s) 13, 425, 551, 710, 917, 922, 1085, 1121
Bsp19I CCATGG 1 cut(s) 1043
BspACI CCGC 4 cut(s) 50, 92, 376, 645
BspANI GGCC 3 cut(s) 219, 370, 382
BspCNI CTCAG 1 cut(s) 1115
BspLI GGNNCC 6 cut(s) 253, 664, 718, 804, 1074, 1203
BspMAI CTGCAG 2 cut(s) 265, 1211
BspPI GGATC 4 cut(s) 21, 433, 705, 1116
BspT104I TTCGAA 1 cut(s) 274
BspT107I GGYRCC 3 cut(s) 251, 802, 1201
BsrDI GCAATG 1 cut(s) 118
BsrFI RCCGGY 1 cut(s) 749
BsrI ACTGG 1 cut(s) 525
BssAI RCCGGY 1 cut(s) 749
BssECI CCNNGG 4 cut(s) 126, 220, 365, 1043
BssMI GATC 8 cut(s) 13, 425, 551, 710, 917, 922, 1085, 1121
BssNAI GTATAC 1 cut(s) 144
BssNI GRCGYC 1 cut(s) 229
BssSI CACGAG 1 cut(s) 1311
BssT1I CCWWGG 2 cut(s) 126, 1043
Bst1107I GTATAC 1 cut(s) 144
Bst2BI CACGAG 1 cut(s) 1311
Bst2UI CCWGG 1 cut(s) 831
Bst4CI ACNGT 4 cut(s) 74, 141, 730, 796
Bst6I CTCTTC 1 cut(s) 234
BstACI GRCGYC 1 cut(s) 229
BstAPI GCANNNNNTGC 1 cut(s) 986
BstBAI YACGTR 2 cut(s) 401, 628
BstBI TTCGAA 1 cut(s) 274
BstC8I GCNNGC 1 cut(s) 751
BstDEI CTNAG 5 cut(s) 300, 309, 372, 846, 1102
BstDSI CCRYGG 1 cut(s) 1043
BstEII GGTNACC 1 cut(s) 438
BstENI CCTNNNNNAGG 1 cut(s) 330
BstF5I GGATG 5 cut(s) 388, 443, 520, 641, 695
BstHHI GCGC 2 cut(s) 82, 1161
BstKTI GATC 8 cut(s) 16, 428, 554, 713, 920, 925, 1088, 1124
BstMBI GATC 8 cut(s) 13, 425, 551, 710, 917, 922, 1085, 1121
BstMCI CGRYCG 1 cut(s) 220
BstMWI GCNNNNNNNGC 2 cut(s) 47, 986
BstNI CCWGG 1 cut(s) 831
BstNSI RCATGY 2 cut(s) 586, 984
BstPI GGTNACC 1 cut(s) 438
BstSCI CCNGG 2 cut(s) 365, 829
BstSFI CTRYAG 2 cut(s) 261, 1207
BstSLI GKGCMC 1 cut(s) 1206
BstV1I GCAGC 2 cut(s) 517, 1241
BstV2I GAAGAC 3 cut(s) 30, 879, 1293
BstX2I RGATCY 3 cut(s) 551, 710, 1121
BstYI RGATCY 3 cut(s) 551, 710, 1121
BstZ17I GTATAC 1 cut(s) 144
BstZI CGGCCG 1 cut(s) 217
BsuRI GGCC 3 cut(s) 219, 370, 382
BtgI CCRYGG 1 cut(s) 1043
BtrI CACGTC 1 cut(s) 1081
BtsCI GGATG 5 cut(s) 388, 443, 520, 641, 695
BtsI GCAGTG 1 cut(s) 1143
BtsIMutI CAGTG 4 cut(s) 414, 576, 726, 1143
Cac8I GCNNGC 1 cut(s) 751
CfoI GCGC 2 cut(s) 82, 1161
Cfr10I RCCGGY 1 cut(s) 749
Cfr13I GGNCC 4 cut(s) 323, 663, 676, 1072
CseI GACGC 1 cut(s) 218
CsiI ACCWGGT 1 cut(s) 829
Csp6I GTAC 7 cut(s) 214, 494, 518, 573, 735, 803, 1234
CviQI GTAC 7 cut(s) 214, 494, 518, 573, 735, 803, 1234
DdeI CTNAG 5 cut(s) 300, 309, 372, 846, 1102
DpnI GATC 8 cut(s) 15, 427, 553, 712, 919, 924, 1087, 1123
DpnII GATC 8 cut(s) 13, 425, 551, 710, 917, 922, 1085, 1121
DraIII CACNNNGTG 3 cut(s) 730, 833, 950
DrdI GACNNNNNNGTC 1 cut(s) 1079
DseDI GACNNNNNNGTC 1 cut(s) 1079
EaeI YGGCCR 1 cut(s) 217
EagI CGGCCG 1 cut(s) 217
Eam1104I CTCTTC 1 cut(s) 234
EarI CTCTTC 1 cut(s) 234
EclXI CGGCCG 1 cut(s) 217
Eco130I CCWWGG 2 cut(s) 126, 1043
Eco147I AGGCCT 1 cut(s) 382
Eco24I GRGCYC 1 cut(s) 1312
Eco47I GGWCC 4 cut(s) 323, 663, 676, 1072
Eco52I CGGCCG 1 cut(s) 217
Eco57I CTGAAG 3 cut(s) 474, 759, 1050
Eco91I GGTNACC 1 cut(s) 438
EcoNI CCTNNNNNAGG 1 cut(s) 330
EcoO109I RGGNCCY 1 cut(s) 663
EcoO65I GGTNACC 1 cut(s) 438
EcoRII CCWGG 1 cut(s) 829
EcoT14I CCWWGG 2 cut(s) 126, 1043
EcoT38I GRGCYC 1 cut(s) 1312
ErhI CCWWGG 2 cut(s) 126, 1043
FaqI GGGAC 1 cut(s) 1310
FblI GTMKAC 1 cut(s) 143
Fnu4HI GCNGC 2 cut(s) 531, 1230
FokI GGATG 5 cut(s) 375, 430, 527, 628, 682
FriOI GRGCYC 1 cut(s) 1312
Fsp4HI GCNGC 2 cut(s) 531, 1230
FspBI CTAG 1 cut(s) 327
FspI TGCGCA 1 cut(s) 81
GlaI GCGC 2 cut(s) 81, 1160
GluI GCNGC 2 cut(s) 531, 1230
HaeIII GGCC 3 cut(s) 219, 370, 382
HapII CCGG 3 cut(s) 367, 714, 750
HgaI GACGC 1 cut(s) 218
HhaI GCGC 2 cut(s) 82, 1161
Hin1I GRCGYC 1 cut(s) 229
Hin6I GCGC 2 cut(s) 80, 1159
HinP1I GCGC 2 cut(s) 80, 1159
HincII GTYRAC 1 cut(s) 855
HindII GTYRAC 1 cut(s) 855
HindIII AAGCTT 1 cut(s) 899
HinfI GANTC 6 cut(s) 314, 355, 509, 809, 842, 1175
HpaII CCGG 3 cut(s) 367, 714, 750
HphI GGTGA 4 cut(s) 86, 420, 432, 1163
Hpy166II GTNNAC 4 cut(s) 144, 323, 855, 1214
Hpy188I TCNGA 7 cut(s) 18, 189, 344, 514, 814, 922, 1085
Hpy188III TCNNGA 2 cut(s) 915, 1028
Hpy8I GTNNAC 4 cut(s) 144, 323, 855, 1214
HpyAV CCTTC 4 cut(s) 326, 783, 1213, 1299
HpyCH4III ACNGT 4 cut(s) 74, 141, 730, 796
HpyCH4IV ACGT 4 cut(s) 400, 627, 966, 1080
HpyCH4V TGCA 9 cut(s) 63, 122, 263, 388, 586, 836, 1136, 1209, 1229
HpyF10VI GCNNNNNNNGC 2 cut(s) 47, 986
HpyF3I CTNAG 5 cut(s) 300, 309, 372, 846, 1102
HpySE526I ACGT 4 cut(s) 400, 627, 966, 1080
Hsp92I GRCGYC 1 cut(s) 229
HspAI GCGC 2 cut(s) 80, 1159
KpnI GGTACC 1 cut(s) 806
KroI GCCGGC 1 cut(s) 749
KroNI GCCGGC 1 cut(s) 751
Kzo9I GATC 8 cut(s) 13, 425, 551, 710, 917, 922, 1085, 1121
LmnI GCTCC 2 cut(s) 181, 1039
Lsp1109I GCAGC 2 cut(s) 517, 1241
LweI GCATC 5 cut(s) 67, 193, 345, 397, 736
MabI ACCWGGT 1 cut(s) 829
MaeI CTAG 1 cut(s) 327
MaeII ACGT 4 cut(s) 400, 627, 966, 1080
MaeIII GTNAC 2 cut(s) 438, 1091
MalI GATC 8 cut(s) 15, 427, 553, 712, 919, 924, 1087, 1123
MboI GATC 8 cut(s) 13, 425, 551, 710, 917, 922, 1085, 1121
MfeI CAATTG 1 cut(s) 680
MflI RGATCY 3 cut(s) 551, 710, 1121
MhlI GDGCHC 2 cut(s) 1206, 1312
MlyI GAGTC 1 cut(s) 803
MmeI TCCRAC 2 cut(s) 34, 660
MroNI GCCGGC 1 cut(s) 749
MroXI GAANNNNTTC 1 cut(s) 690
MseI TTAA 2 cut(s) 777, 941
MslI CAYNNNNRTG 2 cut(s) 581, 979
MspA1I CMGCKG 1 cut(s) 647
MspI CCGG 3 cut(s) 367, 714, 750
MspR9I CCNGG 2 cut(s) 367, 831
MunI CAATTG 1 cut(s) 680
MvaI CCWGG 1 cut(s) 831
MwoI GCNNNNNNNGC 2 cut(s) 47, 986
NaeI GCCGGC 1 cut(s) 751
NciI CCSGG 1 cut(s) 367
NcoI CCATGG 1 cut(s) 1043
NdeII GATC 8 cut(s) 13, 425, 551, 710, 917, 922, 1085, 1121
NgoMIV GCCGGC 1 cut(s) 749
NlaIV GGNNCC 6 cut(s) 253, 664, 718, 804, 1074, 1203
NmeAIII GCCGAG 1 cut(s) 245
NmuCI GTSAC 1 cut(s) 438
NsbI TGCGCA 1 cut(s) 81
NspI RCATGY 2 cut(s) 586, 984
NspV TTCGAA 1 cut(s) 274
PceI AGGCCT 1 cut(s) 382
PdiI GCCGGC 1 cut(s) 751
PdmI GAANNNNTTC 1 cut(s) 690
PfeI GAWTC 5 cut(s) 314, 355, 509, 842, 1175
PkrI GCNGC 2 cut(s) 532, 1231
PleI GAGTC 1 cut(s) 803
PpsI GAGTC 1 cut(s) 803
Ppu21I YACGTR 2 cut(s) 401, 628
PpuMI RGGWCCY 1 cut(s) 663
Psp5II RGGWCCY 1 cut(s) 663
Psp6I CCWGG 1 cut(s) 829
PspEI GGTNACC 1 cut(s) 438
PspGI CCWGG 1 cut(s) 829
PspN4I GGNNCC 6 cut(s) 253, 664, 718, 804, 1074, 1203
PspPI GGNCC 4 cut(s) 323, 663, 676, 1072
PspPPI RGGWCCY 1 cut(s) 663
PstI CTGCAG 2 cut(s) 265, 1211
PsuI RGATCY 3 cut(s) 551, 710, 1121
RsaI GTAC 7 cut(s) 215, 495, 519, 574, 736, 804, 1235
RsaNI GTAC 7 cut(s) 214, 494, 518, 573, 735, 803, 1234
RseI CAYNNNNRTG 2 cut(s) 581, 979
SaqAI TTAA 2 cut(s) 777, 941
SatI GCNGC 2 cut(s) 531, 1230
Sau3AI GATC 8 cut(s) 13, 425, 551, 710, 917, 922, 1085, 1121
Sau96I GGNCC 4 cut(s) 323, 663, 676, 1072
ScaI AGTACT 2 cut(s) 495, 736
SchI GAGTC 1 cut(s) 803
ScrFI CCNGG 2 cut(s) 367, 831
SduI GDGCHC 2 cut(s) 1206, 1312
SexAI ACCWGGT 1 cut(s) 829
SfaNI GCATC 5 cut(s) 67, 193, 345, 397, 736
SfcI CTRYAG 2 cut(s) 261, 1207
SfuI TTCGAA 1 cut(s) 274
SinI GGWCC 4 cut(s) 323, 663, 676, 1072
SmiMI CAYNNNNRTG 2 cut(s) 581, 979
SmlI CTYRAG 1 cut(s) 462
SmoI CTYRAG 1 cut(s) 462
SseBI AGGCCT 1 cut(s) 382
SsiI CCGC 4 cut(s) 50, 92, 376, 645
SspMI CTAG 1 cut(s) 327
StuI AGGCCT 1 cut(s) 382
StyD4I CCNGG 2 cut(s) 365, 829
StyI CCWWGG 2 cut(s) 126, 1043
TaaI ACNGT 4 cut(s) 74, 141, 730, 796
TaiI ACGT 4 cut(s) 403, 630, 969, 1083
TaqI TCGA 3 cut(s) 274, 1173, 1185
TatI WGTACW 3 cut(s) 493, 517, 734
TfiI GAWTC 5 cut(s) 314, 355, 509, 842, 1175
Tru1I TTAA 2 cut(s) 777, 941
Tru9I TTAA 2 cut(s) 777, 941
TscAI CASTG 4 cut(s) 414, 583, 733, 1143
TseFI GTSAC 1 cut(s) 438
TseI GCWGC 2 cut(s) 530, 1229
Tsp45I GTSAC 1 cut(s) 438
TspDTI ATGAA 6 cut(s) 93, 558, 963, 1118, 1167, 1299
TspGWI ACGGA 2 cut(s) 688, 1231
TspRI CASTG 4 cut(s) 414, 583, 733, 1143
VpaK11BI GGWCC 4 cut(s) 323, 663, 676, 1072
XagI CCTNNNNNAGG 1 cut(s) 330
XapI RAATTY 2 cut(s) 270, 689
XceI RCATGY 2 cut(s) 586, 984
XmiI GTMKAC 1 cut(s) 143
XmnI GAANNNNTTC 1 cut(s) 690
XspI CTAG 1 cut(s) 327
ZrmI AGTACT 2 cut(s) 495, 736
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.