pycom15g00430

Glutathione S-transferase, N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0003844
Physical Location & Seq
Reverse (-)
263531 .. 264605
1075 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 528 bp
ATGGAGTCAGATGCGAAGGGGAAGGGGGAGATAAGCGTGGAAGGATACAAGGAAGGCAACACAAAGAAAGCCAGGTTGCAGTCGACGCTTGCTGCCCTTGTCAGTGATCCTATACTTTCCGACGTTCCCAAAAAACCAACCTTATCCGATGTCGACACTCTCATCAGCCTCGAATTGGGCAGCGCCATGCGCATCTCCGTCCTAAAGCTCGGCGGCACTTCCTTCGATGTGGCAGTGATGAATTCTTCTACGATGAAGGATCTTAAGCTTGCAATCAGGAAGAAAATCAATGAGATGGAGCAATCTAAGATGGGTCACCGCCACATTTCATGGAAGCATGTTTGGAGGAATTTTTGCCTCTCATACGACAATGAGAAGCTACTAGATGACAGTGCTCCAGTTCAGCATTTTGGCATACACAACCATTCTCAGGTGCCTTTTGTTCCTTATATTGCTCCCAAGGATTCTTGTAAGCATTCGAAGAGGAGGAGACACTGCTTCTTTCACGGCCTCAGCAAGCTTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00480 ko00980 ko00982 ko00983 ko01524 ko05200 ko05204 ko05225 ko05418 map00480 map00980 map00982 map00983 map01524 map05200 map05204 map05225 map05418
Pfam Domains
Protein Families
GST_N

Protein Analysis

176

Amino Acids

19.63

Weight (kDa)

9.44

Isoelectric Point (pI)

44.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ubiquitin_4 PF18036 63 - 150 4.2e-29 Ubiquitin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 191
AccB1I GGYRCC 1 cut(s) 433
AccI GTMKAC 2 cut(s) 83, 153
AciI CCGC 2 cut(s) 213, 319
AclWI GGATC 2 cut(s) 101, 267
AcsI RAATTY 2 cut(s) 241, 349
AfiI CCNNNNNNNGG 2 cut(s) 175, 430
AflII CTTAAG 1 cut(s) 263
AjnI CCWGG 1 cut(s) 71
AluBI AGCT 4 cut(s) 208, 268, 379, 520
AluI AGCT 4 cut(s) 208, 268, 379, 520
Alw21I GWGCWC 1 cut(s) 397
Alw26I GTCTC 1 cut(s) 484
AlwI GGATC 2 cut(s) 101, 267
AoxI GGCC 1 cut(s) 508
ApeKI GCWGC 2 cut(s) 92, 180
ApoI RAATTY 2 cut(s) 241, 349
AspLEI GCGC 2 cut(s) 185, 192
AsuHPI GGTGA 1 cut(s) 308
AsuII TTCGAA 1 cut(s) 479
BanI GGYRCC 1 cut(s) 433
Bbv12I GWGCWC 1 cut(s) 397
BbvCI CCTCAGC 1 cut(s) 512
BbvI GCAGC 2 cut(s) 79, 192
BccI CCATC 2 cut(s) 289, 304
BceAI ACGGC 1 cut(s) 523
BcgI CGANNNNNNTGC 2 cut(s) 205, 239
BciT130I CCWGG 1 cut(s) 73
BciVI GTATCC 1 cut(s) 38
BcoDI GTCTC 1 cut(s) 484
BfaI CTAG 1 cut(s) 383
BfoI RGCGCY 1 cut(s) 186
BfrI CTTAAG 1 cut(s) 263
BfuI GTATCC 1 cut(s) 38
BisI GCNGC 3 cut(s) 93, 181, 214
BlsI GCNGC 3 cut(s) 94, 182, 215
Bme1390I CCNGG 1 cut(s) 73
BmiI GGNNCC 1 cut(s) 435
BmrFI CCNGG 1 cut(s) 73
BmsI GCATC 1 cut(s) 201
BpmI CTGGAG 1 cut(s) 381
Bpu10I CCTNAGC 1 cut(s) 512
Bpu14I TTCGAA 1 cut(s) 479
BsaJI CCNNGG 1 cut(s) 459
Bsc4I CCNNNNNNNGG 2 cut(s) 175, 430
Bse1I ACTGG 1 cut(s) 398
BseBI CCWGG 1 cut(s) 73
BseDI CCNNGG 1 cut(s) 459
BseLI CCNNNNNNNGG 2 cut(s) 175, 430
BseMII CTCAG 2 cut(s) 443, 526
BseNI ACTGG 1 cut(s) 398
BseRI GAGGAG 2 cut(s) 499, 502
BseXI GCAGC 2 cut(s) 79, 192
BshFI GGCC 1 cut(s) 510
BshNI GGYRCC 1 cut(s) 433
BsiHKAI GWGCWC 1 cut(s) 397
BslI CCNNNNNNNGG 2 cut(s) 175, 430
BsmAI GTCTC 1 cut(s) 484
BsmI GAATGC 1 cut(s) 475
BsnI GGCC 1 cut(s) 510
Bsp119I TTCGAA 1 cut(s) 479
Bsp1286I GDGCHC 1 cut(s) 397
Bsp143I GATC 2 cut(s) 106, 259
BspACI CCGC 2 cut(s) 213, 319
BspANI GGCC 1 cut(s) 510
BspCNI CTCAG 2 cut(s) 442, 525
BspLI GGNNCC 1 cut(s) 435
BspPI GGATC 2 cut(s) 101, 267
BspT104I TTCGAA 1 cut(s) 479
BspT107I GGYRCC 1 cut(s) 433
BspTI CTTAAG 1 cut(s) 263
BsrI ACTGG 1 cut(s) 398
BssECI CCNNGG 1 cut(s) 459
BssMI GATC 2 cut(s) 106, 259
BssT1I CCWWGG 1 cut(s) 459
Bst2UI CCWGG 1 cut(s) 73
Bst4CI ACNGT 1 cut(s) 392
Bst6I CTCTTC 1 cut(s) 476
BstAFI CTTAAG 1 cut(s) 263
BstBI TTCGAA 1 cut(s) 479
BstC8I GCNNGC 3 cut(s) 90, 270, 518
BstDEI CTNAG 3 cut(s) 306, 429, 512
BstEII GGTNACC 1 cut(s) 314
BstH2I RGCGCY 1 cut(s) 186
BstHHI GCGC 2 cut(s) 185, 192
BstKTI GATC 2 cut(s) 109, 262
BstMAI GTCTC 1 cut(s) 484
BstMBI GATC 2 cut(s) 106, 259
BstMWI GCNNNNNNNGC 2 cut(s) 85, 189
BstNI CCWGG 1 cut(s) 73
BstNSI RCATGY 1 cut(s) 341
BstPI GGTNACC 1 cut(s) 314
BstSCI CCNGG 1 cut(s) 71
BstV1I GCAGC 2 cut(s) 79, 192
BstX2I RGATCY 1 cut(s) 259
BstYI RGATCY 1 cut(s) 259
BsuI GTATCC 1 cut(s) 38
BsuRI GGCC 1 cut(s) 510
BtsI GCAGTG 2 cut(s) 240, 493
BtsIMutI CAGTG 4 cut(s) 109, 240, 397, 493
Cac8I GCNNGC 3 cut(s) 90, 270, 518
CfoI GCGC 2 cut(s) 185, 192
CseI GACGC 1 cut(s) 94
CviAII CATG 3 cut(s) 187, 330, 338
CviJI RGCY 7 cut(s) 71, 168, 208, 268, 379, 510, 520
CviKI_1 RGCY 7 cut(s) 71, 168, 208, 268, 379, 510, 520
DdeI CTNAG 3 cut(s) 306, 429, 512
DpnI GATC 2 cut(s) 108, 261
DpnII GATC 2 cut(s) 106, 259
Eam1104I CTCTTC 1 cut(s) 476
EarI CTCTTC 1 cut(s) 476
Eco130I CCWWGG 1 cut(s) 459
Eco91I GGTNACC 1 cut(s) 314
EcoO65I GGTNACC 1 cut(s) 314
EcoRI GAATTC 1 cut(s) 241
EcoRII CCWGG 1 cut(s) 71
EcoT14I CCWWGG 1 cut(s) 459
ErhI CCWWGG 1 cut(s) 459
FaeI CATG 3 cut(s) 190, 333, 341
FaiI YATR 7 cut(s) 113, 188, 331, 339, 364, 416, 450
FatI CATG 3 cut(s) 186, 329, 337
FblI GTMKAC 2 cut(s) 83, 153
Fnu4HI GCNGC 3 cut(s) 93, 181, 214
Fsp4HI GCNGC 3 cut(s) 93, 181, 214
FspAI RTGCGCAY 1 cut(s) 191
FspBI CTAG 1 cut(s) 383
FspI TGCGCA 1 cut(s) 191
GlaI GCGC 2 cut(s) 184, 191
GluI GCNGC 3 cut(s) 93, 181, 214
GsuI CTGGAG 1 cut(s) 381
HaeII RGCGCY 1 cut(s) 186
HaeIII GGCC 1 cut(s) 510
HgaI GACGC 1 cut(s) 94
HhaI GCGC 2 cut(s) 185, 192
Hin1II CATG 3 cut(s) 190, 333, 341
Hin6I GCGC 2 cut(s) 183, 190
HinP1I GCGC 2 cut(s) 183, 190
HincII GTYRAC 2 cut(s) 84, 154
HindII GTYRAC 2 cut(s) 84, 154
HindIII AAGCTT 2 cut(s) 266, 518
HinfI GANTC 2 cut(s) 5, 464
HphI GGTGA 1 cut(s) 308
Hpy166II GTNNAC 2 cut(s) 84, 154
Hpy188I TCNGA 3 cut(s) 10, 121, 148
Hpy188III TCNNGA 2 cut(s) 277, 525
Hpy8I GTNNAC 2 cut(s) 84, 154
Hpy99I CGWCG 2 cut(s) 88, 125
HpyAV CCTTC 6 cut(s) 10, 16, 35, 47, 232, 250
HpyCH4III ACNGT 1 cut(s) 392
HpyCH4IV ACGT 1 cut(s) 123
HpyCH4V TGCA 2 cut(s) 79, 272
HpyF10VI GCNNNNNNNGC 2 cut(s) 85, 189
HpyF3I CTNAG 3 cut(s) 306, 429, 512
HpySE526I ACGT 1 cut(s) 123
Hsp92II CATG 3 cut(s) 190, 333, 341
HspAI GCGC 2 cut(s) 183, 190
Kzo9I GATC 2 cut(s) 106, 259
LmnI GCTCC 3 cut(s) 298, 400, 460
LpnPI CCDG 5 cut(s) 58, 85, 262, 411, 416
Lsp1109I GCAGC 2 cut(s) 79, 192
LweI GCATC 1 cut(s) 201
MaeI CTAG 1 cut(s) 383
MaeII ACGT 1 cut(s) 123
MaeIII GTNAC 1 cut(s) 314
MalI GATC 2 cut(s) 108, 261
MboI GATC 2 cut(s) 106, 259
MboII GAAGA 3 cut(s) 237, 292, 493
MflI RGATCY 1 cut(s) 259
MhlI GDGCHC 1 cut(s) 397
MluCI AATT 3 cut(s) 173, 241, 349
MlyI GAGTC 1 cut(s) 14
MmeI TCCRAC 1 cut(s) 144
MnlI CCTC 6 cut(s) 179, 339, 368, 477, 480, 521
MseI TTAA 1 cut(s) 264
MspCI CTTAAG 1 cut(s) 263
MspR9I CCNGG 1 cut(s) 73
Mva1269I GAATGC 1 cut(s) 475
MvaI CCWGG 1 cut(s) 73
MwoI GCNNNNNNNGC 2 cut(s) 85, 189
NdeII GATC 2 cut(s) 106, 259
NlaIII CATG 3 cut(s) 190, 333, 341
NlaIV GGNNCC 1 cut(s) 435
NmeAIII GCCGAG 1 cut(s) 189
NmuCI GTSAC 1 cut(s) 314
NsbI TGCGCA 1 cut(s) 191
NspI RCATGY 1 cut(s) 341
NspV TTCGAA 1 cut(s) 479
PctI GAATGC 1 cut(s) 475
PfeI GAWTC 1 cut(s) 464
PkrI GCNGC 3 cut(s) 94, 182, 215
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
Psp6I CCWGG 1 cut(s) 71
PspEI GGTNACC 1 cut(s) 314
PspGI CCWGG 1 cut(s) 71
PspN4I GGNNCC 1 cut(s) 435
PsuI RGATCY 1 cut(s) 259
SalI GTCGAC 2 cut(s) 82, 152
SaqAI TTAA 1 cut(s) 264
SatI GCNGC 3 cut(s) 93, 181, 214
Sau3AI GATC 2 cut(s) 106, 259
SchI GAGTC 1 cut(s) 14
ScrFI CCNGG 1 cut(s) 73
SduI GDGCHC 1 cut(s) 397
SetI ASST 8 cut(s) 77, 126, 143, 210, 270, 381, 435, 522
SfaNI GCATC 1 cut(s) 201
SfuI TTCGAA 1 cut(s) 479
SmlI CTYRAG 1 cut(s) 263
SmoI CTYRAG 1 cut(s) 263
Sse9I AATT 3 cut(s) 173, 241, 349
SsiI CCGC 2 cut(s) 213, 319
SspMI CTAG 1 cut(s) 383
StyD4I CCNGG 1 cut(s) 71
StyI CCWWGG 1 cut(s) 459
TaaI ACNGT 1 cut(s) 392
TaiI ACGT 1 cut(s) 126
TaqI TCGA 5 cut(s) 83, 153, 171, 225, 479
TasI AATT 3 cut(s) 173, 241, 349
TauI GCSGC 1 cut(s) 216
TfiI GAWTC 1 cut(s) 464
Tru1I TTAA 1 cut(s) 264
Tru9I TTAA 1 cut(s) 264
TscAI CASTG 4 cut(s) 109, 240, 397, 500
TseFI GTSAC 1 cut(s) 314
TseI GCWGC 2 cut(s) 92, 180
Tsp45I GTSAC 1 cut(s) 314
TspDTI ATGAA 3 cut(s) 254, 269, 318
TspGWI ACGGA 1 cut(s) 187
TspRI CASTG 4 cut(s) 109, 240, 397, 500
Vha464I CTTAAG 1 cut(s) 263
XapI RAATTY 2 cut(s) 241, 349
XceI RCATGY 1 cut(s) 341
XmiI GTMKAC 2 cut(s) 83, 153
XspI CTAG 1 cut(s) 383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.