pycom15g02350

DUF21 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0009069
Physical Location & Seq
Forward (+)
1379142 .. 1383118
3977 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1863 bp
ATGATGAATTTAAATTACTACGGTCTTGTAATATTCATCTTCACATGTAATATATTATTACTAGTTTATTATAAGGTTAAGTTCTCATCTTTCTTCTGCCGCGTTCTAATCGTCTCAATTAAACGCAAGTTATTCTTGTTAACGCGGGCACTTGCTCCACTGGCGCCGCTAAAAGCAAGTGTTTTTGTGGAAGCAAATAAAAAAATCGAGGGAACCGAACCGGCCAAAAAGAGATCCAGTACACCCAACCGAAAAGGTCCGAGAAACGCGCACGGCTTCAAATTTTGGTCTCCACTCTCCAGCAACCGAGCGACGGAGATGGCTGCGGTGGTGGCCTGCTGCGGGACGAAGTTCTTTTTGTACGTGCTGATAATCGTAGGCCTGGTATGCTTCGCCGGAATGATGGCCGGACTCACTCTCGGCCTCATGTCCCTCGGCCTCGTCGACCTCGAGGTCCTCATGAAGTCCGGCCGCCCTCAGGATCGCAAACACGCCGCTAAAATTTATCCGGTGGTGAAGAATCAGCACCTGTTACTTTGCACGCTTTTAATCGGAAACGCGTTAGCAATGGAGGCTCTTCCGGTGTTTTTGGATAGTCTTGTGCCTCCTTGGGCGGCCATTGTGATATCAGTCACTCTCATCCTGGTGTTTGGGGAGATATTGCCGCAAGCAGTCTGCACTCGCTATGGATTGACTGTTGGGGCGACAATGGCCCCATTTGTGCGCTTTCTTCTTGTTCTTTTCCTCCCCATTTCGTATCCCATTAGTAAGGTTCTGGATTATATGCTGGGTAAGGGACAATCTGCCCTTTTACGAAGAGCAGAGCTAAAGACTTTTGTGAATTTTCATGGAAATGAGGCTGGGAAAGGCGGGGATTTAACACATGATGAGACTACCATCATTGCTGGGGCACTTGAATTGACTGAAAAGACAGCAAAAGATGCTATGACTCCCATAACAAATGCATTTGCCCTTGATCTGGATGCACCTCTTACTTTGGACACATTGAATGCAATAATGGCAATGGGTCATAGTAGAGTTCCAGTTTATTGTGGGAAGCCCGGAAATATAATTGGTCTTGTTCTGGTTAAGAATCTCTTGATGGTTGATCCAGAAGGTGGAGTTACTTTGAGAAAACTGATGATCAGAAAAATTCCTCGGGTTTCAGAAGACATGCCTCTGTATGATATTCTAAATGAATTTCAGAAGGGTCACAGTCACATTGCTGTTGTCTTTAAGTATCCAAACGGGAAAAAGGATGCACTAAAGAGTGGCAAAGATGGCGAACCACCCGTGTTTAAGGATGATTGTAAGAAGCAAGCGGGCCAACCTGTGGCATTATTCAAGAAAGATGAAAAAGTGGGTTCAAGCAATGCTCTGAGTGCTTTGGACAATGGTGATGGAGGTCAACAATCTAAGAAAAGCCCATCAGCTACTCCCGCTTTTAAGAAACGACACAGAGGCTGTTCACATTGCATTCTGGACGTTGAGAACGGTCCCATTCCAGTTTTCCCAAACAATGAAGAGTTTGTAGGAGTAATTACCATGGAGGATGTCATTGAAGAACTTCTTCAGGAAGAGATATGGGATGAAACAGATGAGTACGTCGATATCCACAACAGGATTAAGGTCAATATGCATGCATCTCAGGAAAAGCCTTCTTACACGAACTCATCCAATCCTTCCGGACAATGGTCCCCTGTCAACCACGCCCCACTACTAACATCATCATCAGTGCCATCATATTCAGGTTCTTCTCAACATCAAGAGTCTGATAAGTTGACTGCTGTAGGTCCAATGCCAACGGACTCACATACCACAACTCATCAAGGTTCTGAAGGCGACACGTCAAGAGATCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBS DUF21

Protein Analysis

621

Amino Acids

67.82

Weight (kDa)

8.66

Isoelectric Point (pI)

41.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CNNM PF01595 123 - 293 7.4e-34 Cyclin M transmembrane N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 72
AasI GACNNNNNNGTC 1 cut(s) 452
AbsI CCTCGAGG 1 cut(s) 449
AccB1I GGYRCC 1 cut(s) 163
AccB7I CCANNNNNTGG 2 cut(s) 1118, 1333
AccI GTMKAC 1 cut(s) 444
AccII CGCG 4 cut(s) 102, 145, 269, 560
AccIII TCCGGA 1 cut(s) 1685
AclWI GGATC 3 cut(s) 228, 489, 1103
AcoI YGGCCR 4 cut(s) 222, 405, 469, 615
AcsI RAATTY 6 cut(s) 7, 281, 501, 841, 1152, 1199
AcuI CTGAAG 2 cut(s) 1556, 1857
AcyI GRCGYC 1 cut(s) 164
AfaI GTAC 3 cut(s) 241, 362, 1604
AfiI CCNNNNNNNGG 7 cut(s) 313, 342, 478, 979, 1118, 1333, 1692
AflIII ACRYGT 3 cut(s) 44, 558, 1845
AgsI TTSAA 6 cut(s) 280, 917, 1009, 1345, 1368, 1562
AhlI ACTAGT 1 cut(s) 61
AjiI CACGTC 1 cut(s) 1848
AjnI CCWGG 2 cut(s) 381, 642
AluBI AGCT 2 cut(s) 826, 1433
AluI AGCT 2 cut(s) 826, 1433
Alw26I GTCTC 3 cut(s) 118, 294, 884
AlwI GGATC 3 cut(s) 228, 489, 1103
AlwNI CAGNNNCTG 2 cut(s) 529, 1464
Ama87I CYCGRG 2 cut(s) 449, 1158
Aor13HI TCCGGA 1 cut(s) 1685
ApeKI GCWGC 2 cut(s) 323, 339
ApoI RAATTY 6 cut(s) 7, 281, 501, 841, 1152, 1199
Asp700I GAANNNNTTC 2 cut(s) 1566, 1569
AspLEI GCGC 3 cut(s) 166, 271, 726
AspS9I GGNCC 7 cut(s) 257, 454, 712, 1324, 1496, 1695, 1793
AsuC2I CCSGG 1 cut(s) 1062
AsuHPI GGTGA 2 cut(s) 526, 1409
AvaI CYCGRG 2 cut(s) 449, 1158
AvaII GGWCC 5 cut(s) 257, 454, 1496, 1695, 1793
AxyI CCTNAGG 1 cut(s) 477
BaeGI GKGCMC 2 cut(s) 151, 913
BanI GGYRCC 1 cut(s) 163
BbsI GAAGAC 1 cut(s) 1176
BbvI GCAGC 2 cut(s) 310, 326
BccI CCATC 8 cut(s) 313, 397, 905, 1096, 1274, 1394, 1435, 1747
BceAI ACGGC 1 cut(s) 289
BciT130I CCWGG 2 cut(s) 383, 644
BciVI GTATCC 2 cut(s) 768, 1251
BclI TGATCA 1 cut(s) 1143
BcnI CCSGG 1 cut(s) 1062
BcoDI GTCTC 3 cut(s) 118, 294, 884
BcuI ACTAGT 1 cut(s) 61
BfaI CTAG 1 cut(s) 62
BfmI CTRYAG 1 cut(s) 1788
BfoI RGCGCY 1 cut(s) 167
BfuI GTATCC 2 cut(s) 768, 1251
BisI GCNGC 8 cut(s) 100, 167, 324, 340, 472, 495, 615, 665
BlsI GCNGC 8 cut(s) 101, 168, 325, 341, 473, 496, 616, 666
Bme1390I CCNGG 3 cut(s) 383, 644, 1062
Bme18I GGWCC 5 cut(s) 257, 454, 1496, 1695, 1793
BmeT110I CYCGRG 2 cut(s) 449, 1158
BmgBI CACGTC 1 cut(s) 1848
BmgT120I GGNCC 7 cut(s) 257, 454, 712, 1324, 1496, 1695, 1793
BmiI GGNNCC 5 cut(s) 165, 214, 714, 1498, 1697
BmrFI CCNGG 3 cut(s) 383, 644, 1062
BmsI GCATC 4 cut(s) 931, 973, 1249, 1652
BoxI GACNNNNGTC 1 cut(s) 1693
BpiI GAAGAC 1 cut(s) 1176
BpmI CTGGAG 1 cut(s) 283
BpuMI CCSGG 1 cut(s) 1062
BsaAI YACGTR 1 cut(s) 364
BsaHI GRCGYC 1 cut(s) 164
BsaI GGTCTC 1 cut(s) 294
BsaJI CCNNGG 4 cut(s) 433, 608, 1157, 1545
BsaWI WCCGGW 3 cut(s) 508, 580, 1685
Bsc4I CCNNNNNNNGG 7 cut(s) 313, 342, 478, 979, 1118, 1333, 1692
Bse118I RCCGGY 1 cut(s) 220
Bse1I ACTGG 4 cut(s) 165, 237, 1043, 1505
Bse21I CCTNAGG 1 cut(s) 477
Bse3DI GCAATG 6 cut(s) 573, 900, 1029, 1221, 1378, 1471
BseAI TCCGGA 1 cut(s) 1685
BseBI CCWGG 2 cut(s) 383, 644
BseDI CCNNGG 4 cut(s) 433, 608, 1157, 1545
BseGI GGATG 7 cut(s) 639, 988, 1264, 1309, 1558, 1594, 1673
BseLI CCNNNNNNNGG 7 cut(s) 313, 342, 478, 979, 1118, 1333, 1692
BseMI GCAATG 6 cut(s) 573, 900, 1029, 1221, 1378, 1471
BseMII CTCAG 3 cut(s) 491, 1370, 1661
BseNI ACTGG 4 cut(s) 165, 237, 1043, 1505
BseSI GKGCMC 2 cut(s) 151, 913
BseX3I CGGCCG 1 cut(s) 469
BseXI GCAGC 2 cut(s) 310, 326
BseYI CCCAGC 3 cut(s) 787, 860, 905
BsgI GTGCAG 1 cut(s) 661
Bsh1236I CGCG 4 cut(s) 102, 145, 269, 560
Bsh1285I CGRYCG 1 cut(s) 472
BshNI GGYRCC 1 cut(s) 163
BsiEI CGRYCG 1 cut(s) 472
BsiHKCI CYCGRG 2 cut(s) 449, 1158
BsiSI CCGG 8 cut(s) 221, 396, 408, 468, 509, 581, 1062, 1686
BslFI GGGAC 5 cut(s) 358, 415, 810, 1482, 1681
BslI CCNNNNNNNGG 7 cut(s) 313, 342, 478, 979, 1118, 1333, 1692
BsmAI GTCTC 3 cut(s) 118, 294, 884
BsmBI CGTCTC 1 cut(s) 118
BsmFI GGGAC 5 cut(s) 358, 415, 810, 1482, 1681
BsmI GAATGC 2 cut(s) 1015, 1476
Bso31I GGTCTC 1 cut(s) 294
BsoBI CYCGRG 2 cut(s) 449, 1158
Bsp1286I GDGCHC 2 cut(s) 151, 913
Bsp13I TCCGGA 1 cut(s) 1685
Bsp143I GATC 6 cut(s) 233, 481, 976, 1108, 1143, 1855
Bsp19I CCATGG 1 cut(s) 1545
BspCNI CTCAG 3 cut(s) 490, 1371, 1660
BspEI TCCGGA 1 cut(s) 1685
BspFNI CGCG 4 cut(s) 102, 145, 269, 560
BspHI TCATGA 1 cut(s) 459
BspLI GGNNCC 5 cut(s) 165, 214, 714, 1498, 1697
BspPI GGATC 3 cut(s) 228, 489, 1103
BspQI GCTCTTC 2 cut(s) 582, 811
BspT107I GGYRCC 1 cut(s) 163
BspTNI GGTCTC 1 cut(s) 294
BsrDI GCAATG 6 cut(s) 573, 900, 1029, 1221, 1378, 1471
BsrFI RCCGGY 1 cut(s) 220
BsrI ACTGG 4 cut(s) 165, 237, 1043, 1505
BssAI RCCGGY 1 cut(s) 220
BssECI CCNNGG 4 cut(s) 433, 608, 1157, 1545
BssMI GATC 6 cut(s) 233, 481, 976, 1108, 1143, 1855
BssNI GRCGYC 1 cut(s) 164
BssT1I CCWWGG 2 cut(s) 608, 1545
Bst2UI CCWGG 2 cut(s) 383, 644
Bst4CI ACNGT 4 cut(s) 23, 697, 1217, 1496
Bst6I CTCTTC 4 cut(s) 582, 811, 1518, 1572
BstACI GRCGYC 1 cut(s) 164
BstAPI GCANNNNNTGC 1 cut(s) 941
BstBAI YACGTR 1 cut(s) 364
BstC8I GCNNGC 7 cut(s) 147, 337, 542, 669, 1320, 1324, 1641
BstDEI CTNAG 4 cut(s) 477, 1379, 1416, 1647
BstDSI CCRYGG 1 cut(s) 1545
BstF5I GGATG 7 cut(s) 639, 988, 1264, 1309, 1558, 1594, 1673
BstFNI CGCG 4 cut(s) 102, 145, 269, 560
BstH2I RGCGCY 1 cut(s) 167
BstHHI GCGC 3 cut(s) 166, 271, 726
BstKTI GATC 6 cut(s) 236, 484, 979, 1111, 1146, 1858
BstMAI GTCTC 3 cut(s) 118, 294, 884
BstMBI GATC 6 cut(s) 233, 481, 976, 1108, 1143, 1855
BstMCI CGRYCG 1 cut(s) 472
BstNI CCWGG 2 cut(s) 383, 644
BstNSI RCATGY 3 cut(s) 48, 1177, 1643
BstPAI GACNNNNGTC 1 cut(s) 1693
BstSCI CCNGG 3 cut(s) 381, 642, 1060
BstSFI CTRYAG 1 cut(s) 1788
BstSLI GKGCMC 2 cut(s) 151, 913
BstUI CGCG 4 cut(s) 102, 145, 269, 560
BstV1I GCAGC 2 cut(s) 310, 326
BstV2I GAAGAC 1 cut(s) 1176
BstX2I RGATCY 1 cut(s) 233
BstYI RGATCY 1 cut(s) 233
BstZI CGGCCG 1 cut(s) 469
Bsu36I CCTNAGG 1 cut(s) 477
BsuI GTATCC 2 cut(s) 768, 1251
BtgI CCRYGG 1 cut(s) 1545
BtrI CACGTC 1 cut(s) 1848
BtsCI GGATG 7 cut(s) 639, 988, 1264, 1309, 1558, 1594, 1673
BtsIMutI CAGTG 2 cut(s) 158, 1740
Cac8I GCNNGC 7 cut(s) 147, 337, 542, 669, 1320, 1324, 1641
CaiI CAGNNNCTG 2 cut(s) 529, 1464
CciI TCATGA 1 cut(s) 459
CfoI GCGC 3 cut(s) 166, 271, 726
Cfr10I RCCGGY 1 cut(s) 220
Cfr13I GGNCC 7 cut(s) 257, 454, 712, 1324, 1496, 1695, 1793
Csp6I GTAC 3 cut(s) 240, 361, 1603
CviAII CATG 8 cut(s) 45, 427, 460, 848, 884, 1174, 1546, 1640
CviQI GTAC 3 cut(s) 240, 361, 1603
DdeI CTNAG 4 cut(s) 477, 1379, 1416, 1647
DinI GGCGCC 1 cut(s) 165
DpnI GATC 6 cut(s) 235, 483, 978, 1110, 1145, 1857
DpnII GATC 6 cut(s) 233, 481, 976, 1108, 1143, 1855
DraI TTTAAA 1 cut(s) 12
DrdI GACNNNNNNGTC 1 cut(s) 452
DseDI GACNNNNNNGTC 1 cut(s) 452
EaeI YGGCCR 4 cut(s) 222, 405, 469, 615
EagI CGGCCG 1 cut(s) 469
Eam1104I CTCTTC 4 cut(s) 582, 811, 1518, 1572
EarI CTCTTC 4 cut(s) 582, 811, 1518, 1572
EclXI CGGCCG 1 cut(s) 469
Eco130I CCWWGG 2 cut(s) 608, 1545
Eco147I AGGCCT 1 cut(s) 381
Eco31I GGTCTC 1 cut(s) 294
Eco32I GATATC 2 cut(s) 627, 1612
Eco47I GGWCC 5 cut(s) 257, 454, 1496, 1695, 1793
Eco52I CGGCCG 1 cut(s) 469
Eco57I CTGAAG 2 cut(s) 1556, 1857
Eco81I CCTNAGG 1 cut(s) 477
Eco88I CYCGRG 2 cut(s) 449, 1158
EcoO109I RGGNCCY 1 cut(s) 454
EcoRII CCWGG 2 cut(s) 381, 642
EcoRV GATATC 2 cut(s) 627, 1612
EcoT14I CCWWGG 2 cut(s) 608, 1545
EcoT22I ATGCAT 3 cut(s) 967, 1641, 1645
EgeI GGCGCC 1 cut(s) 165
EheI GGCGCC 1 cut(s) 165
ErhI CCWWGG 2 cut(s) 608, 1545
Esp3I CGTCTC 1 cut(s) 118
FaeI CATG 8 cut(s) 48, 430, 463, 851, 887, 1177, 1549, 1643
FalI AAGNNNNNCTT 6 cut(s) 119, 151, 1082, 1114, 1554, 1586
FaqI GGGAC 5 cut(s) 358, 415, 810, 1482, 1681
FatI CATG 8 cut(s) 44, 426, 459, 847, 883, 1173, 1545, 1639
FauI CCCGC 5 cut(s) 138, 335, 863, 1315, 1447
FbaI TGATCA 1 cut(s) 1143
FblI GTMKAC 1 cut(s) 444
Fnu4HI GCNGC 8 cut(s) 100, 167, 324, 340, 472, 495, 615, 665
FokI GGATG 7 cut(s) 626, 995, 1271, 1316, 1565, 1601, 1660
Fsp4HI GCNGC 8 cut(s) 100, 167, 324, 340, 472, 495, 615, 665
FspBI CTAG 1 cut(s) 62
GlaI GCGC 3 cut(s) 165, 270, 725
GluI GCNGC 8 cut(s) 100, 167, 324, 340, 472, 495, 615, 665
GsaI CCCAGC 3 cut(s) 791, 864, 909
GsuI CTGGAG 1 cut(s) 283
HaeII RGCGCY 1 cut(s) 167
HapII CCGG 8 cut(s) 221, 396, 408, 468, 509, 581, 1062, 1686
HhaI GCGC 3 cut(s) 166, 271, 726
Hin1I GRCGYC 1 cut(s) 164
Hin1II CATG 8 cut(s) 48, 430, 463, 851, 887, 1177, 1549, 1643
Hin6I GCGC 3 cut(s) 164, 269, 724
HinP1I GCGC 3 cut(s) 164, 269, 724
HincII GTYRAC 5 cut(s) 141, 445, 1409, 1705, 1782
HindII GTYRAC 5 cut(s) 141, 445, 1409, 1705, 1782
HinfI GANTC 6 cut(s) 411, 520, 949, 1093, 1769, 1808
HpaI GTTAAC 1 cut(s) 141
HpaII CCGG 8 cut(s) 221, 396, 408, 468, 509, 581, 1062, 1686
HphI GGTGA 2 cut(s) 526, 1409
Hpy166II GTNNAC 7 cut(s) 141, 242, 445, 1409, 1469, 1705, 1782
Hpy188I TCNGA 8 cut(s) 261, 554, 1148, 1168, 1206, 1380, 1774, 1837
Hpy8I GTNNAC 7 cut(s) 141, 242, 445, 1409, 1469, 1705, 1782
Hpy99I CGWCG 3 cut(s) 316, 446, 1610
HpyAV CCTTC 5 cut(s) 1109, 1201, 1668, 1692, 1832
HpyCH4III ACNGT 4 cut(s) 23, 697, 1217, 1496
HpyCH4IV ACGT 4 cut(s) 363, 1485, 1605, 1847
HpyCH4V TGCA 9 cut(s) 540, 678, 965, 986, 1013, 1262, 1476, 1639, 1643
HpyF3I CTNAG 4 cut(s) 477, 1379, 1416, 1647
HpySE526I ACGT 4 cut(s) 363, 1485, 1605, 1847
Hsp92I GRCGYC 1 cut(s) 164
Hsp92II CATG 8 cut(s) 48, 430, 463, 851, 887, 1177, 1549, 1643
HspAI GCGC 3 cut(s) 164, 269, 724
KasI GGCGCC 1 cut(s) 163
Kpn2I TCCGGA 1 cut(s) 1685
Ksp22I TGATCA 1 cut(s) 1143
KspAI GTTAAC 1 cut(s) 141
Kzo9I GATC 6 cut(s) 233, 481, 976, 1108, 1143, 1855
LguI GCTCTTC 2 cut(s) 582, 811
LmnI GCTCC 1 cut(s) 160
Lsp1109I GCAGC 2 cut(s) 310, 326
LweI GCATC 4 cut(s) 931, 973, 1249, 1652
MaeI CTAG 1 cut(s) 62
MaeII ACGT 4 cut(s) 363, 1485, 1605, 1847
MaeIII GTNAC 5 cut(s) 531, 631, 1123, 1211, 1217
MalI GATC 6 cut(s) 235, 483, 978, 1110, 1145, 1857
MboI GATC 6 cut(s) 233, 481, 976, 1108, 1143, 1855
MflI RGATCY 1 cut(s) 233
MhlI GDGCHC 2 cut(s) 151, 913
MluI ACGCGT 1 cut(s) 558
Mly113I GGCGCC 1 cut(s) 164
MlyI GAGTC 4 cut(s) 405, 943, 1778, 1802
Mph1103I ATGCAT 3 cut(s) 967, 1641, 1645
MroI TCCGGA 1 cut(s) 1685
MroXI GAANNNNTTC 2 cut(s) 1566, 1569
MslI CAYNNNNRTG 2 cut(s) 644, 852
MspI CCGG 8 cut(s) 221, 396, 408, 468, 509, 581, 1062, 1686
MspR9I CCNGG 3 cut(s) 383, 644, 1062
Mva1269I GAATGC 2 cut(s) 1015, 1476
MvaI CCWGG 2 cut(s) 383, 644
MvnI CGCG 4 cut(s) 102, 145, 269, 560
NarI GGCGCC 1 cut(s) 164
NciI CCSGG 1 cut(s) 1062
NcoI CCATGG 1 cut(s) 1545
NdeII GATC 6 cut(s) 233, 481, 976, 1108, 1143, 1855
NlaIII CATG 8 cut(s) 48, 430, 463, 851, 887, 1177, 1549, 1643
NlaIV GGNNCC 5 cut(s) 165, 214, 714, 1498, 1697
NmeAIII GCCGAG 2 cut(s) 399, 414
NmuCI GTSAC 3 cut(s) 631, 1211, 1217
NsiI ATGCAT 3 cut(s) 967, 1641, 1645
NspI RCATGY 3 cut(s) 48, 1177, 1643
PaeI GCATGC 1 cut(s) 1643
PaeR7I CTCGAG 1 cut(s) 449
PagI TCATGA 1 cut(s) 459
PceI AGGCCT 1 cut(s) 381
PciI ACATGT 1 cut(s) 44
PciSI GCTCTTC 2 cut(s) 582, 811
PcsI WCGNNNNNNNCGW 3 cut(s) 213, 441, 447
PctI GAATGC 2 cut(s) 1015, 1476
PdmI GAANNNNTTC 2 cut(s) 1566, 1569
PfeI GAWTC 2 cut(s) 520, 1093
PflMI CCANNNNNTGG 2 cut(s) 1118, 1333
PkrI GCNGC 8 cut(s) 101, 168, 325, 341, 473, 496, 616, 666
PleI GAGTC 4 cut(s) 405, 943, 1777, 1802
PluTI GGCGCC 1 cut(s) 167
PpsI GAGTC 4 cut(s) 405, 943, 1777, 1802
Ppu21I YACGTR 1 cut(s) 364
PpuMI RGGWCCY 1 cut(s) 454
PscI ACATGT 1 cut(s) 44
PshAI GACNNNNGTC 1 cut(s) 1693
PsiI TTATAA 1 cut(s) 72
Psp5II RGGWCCY 1 cut(s) 454
Psp6I CCWGG 2 cut(s) 381, 642
PspFI CCCAGC 3 cut(s) 787, 860, 905
PspGI CCWGG 2 cut(s) 381, 642
PspN4I GGNNCC 5 cut(s) 165, 214, 714, 1498, 1697
PspPI GGNCC 7 cut(s) 257, 454, 712, 1324, 1496, 1695, 1793
PspPPI RGGWCCY 1 cut(s) 454
PspXI VCTCGAGB 1 cut(s) 449
PstNI CAGNNNCTG 2 cut(s) 529, 1464
PsuI RGATCY 1 cut(s) 233
RsaI GTAC 3 cut(s) 241, 362, 1604
RsaNI GTAC 3 cut(s) 240, 361, 1603
RseI CAYNNNNRTG 2 cut(s) 644, 852
SalI GTCGAC 1 cut(s) 443
SapI GCTCTTC 2 cut(s) 582, 811
SatI GCNGC 8 cut(s) 100, 167, 324, 340, 472, 495, 615, 665
Sau3AI GATC 6 cut(s) 233, 481, 976, 1108, 1143, 1855
Sau96I GGNCC 7 cut(s) 257, 454, 712, 1324, 1496, 1695, 1793
SchI GAGTC 4 cut(s) 405, 943, 1778, 1802
ScrFI CCNGG 3 cut(s) 383, 644, 1062
SduI GDGCHC 2 cut(s) 151, 913
SfaNI GCATC 4 cut(s) 931, 973, 1249, 1652
SfcI CTRYAG 1 cut(s) 1788
SfoI GGCGCC 1 cut(s) 165
Sfr274I CTCGAG 1 cut(s) 449
SinI GGWCC 5 cut(s) 257, 454, 1496, 1695, 1793
SlaI CTCGAG 1 cut(s) 449
SmiI ATTTAAAT 1 cut(s) 12
SmiMI CAYNNNNRTG 2 cut(s) 644, 852
SmlI CTYRAG 1 cut(s) 449
SmoI CTYRAG 1 cut(s) 449
SpeI ACTAGT 1 cut(s) 61
SphI GCATGC 1 cut(s) 1643
SseBI AGGCCT 1 cut(s) 381
SspDI GGCGCC 1 cut(s) 163
SspI AATATT 1 cut(s) 33
SspMI CTAG 1 cut(s) 62
StuI AGGCCT 1 cut(s) 381
StyD4I CCNGG 3 cut(s) 381, 642, 1060
StyI CCWWGG 2 cut(s) 608, 1545
SwaI ATTTAAAT 1 cut(s) 12
TaaI ACNGT 4 cut(s) 23, 697, 1217, 1496
TaiI ACGT 4 cut(s) 366, 1488, 1608, 1850
TaqI TCGA 4 cut(s) 207, 444, 450, 1608
TatI WGTACW 1 cut(s) 239
TauI GCSGC 6 cut(s) 102, 169, 474, 497, 617, 667
TfiI GAWTC 2 cut(s) 520, 1093
TscAI CASTG 2 cut(s) 165, 1740
TseFI GTSAC 3 cut(s) 631, 1211, 1217
TseI GCWGC 2 cut(s) 323, 339
Tsp45I GTSAC 3 cut(s) 631, 1211, 1217
TspDTI ATGAA 8 cut(s) 20, 25, 476, 836, 1212, 1368, 1536, 1605
TspGWI ACGGA 2 cut(s) 329, 1820
TspRI CASTG 2 cut(s) 165, 1740
Van91I CCANNNNNTGG 2 cut(s) 1118, 1333
VpaK11BI GGWCC 5 cut(s) 257, 454, 1496, 1695, 1793
XapI RAATTY 6 cut(s) 7, 281, 501, 841, 1152, 1199
XceI RCATGY 3 cut(s) 48, 1177, 1643
XhoI CTCGAG 1 cut(s) 449
XmiI GTMKAC 1 cut(s) 444
XmnI GAANNNNTTC 2 cut(s) 1566, 1569
XspI CTAG 1 cut(s) 62
Zsp2I ATGCAT 3 cut(s) 967, 1641, 1645
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.