pycom15g12940

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0006537
Physical Location & Seq
Forward (+)
8843537 .. 8846181
2645 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g12940.5

Sequence Viewer

Length: 867 bp
ATGACAACACCCTTGCATTTGAGGCTCTTCCTGCCTCTCACCTCCATTACTCCCTATTCTTCTTCTTCTTCTTCCTCCTCAGACTTCCCCTGCAAGTCCTTCAAATCCTTTCACTCTGCAAGCCCTAATTTCAACCTTTCTTCCCTCTCATCTCACAGCCACACATGTGCTCGAAACAGGACATCCACTCGCTCCAAACCCAAGTGGCTTTGTGGACGCTTGAGAAGTGATCAAGACATAGGTAGTCGATCAAATGAAGACGCGAGCGCGACTGGTGCGAGTATGTTTGGCTCGGATGAAGAAGGAGGTACCCAAATCCCAACCCAGGCTCAATCTGTTGTCGAAGGATCAGGGGCAGTTATGGTTTCAGAGTATAGACCTGTGGACGATGTGGATTATCTACAGGAGTTATTAGCCATTCAGCAACAAGGGCCCAGAGCGATTGGTTTCTTTGGGACCCGGAATATGGGATTCATGCATCAAGAACTTGTTGAGATTCTCAGCTATGCGATGGTGATCACTAAAAATCACATATATACATCAGGGGCATCCGGTACTAATGCAGCTGTCATCAGGGGTGCTTTAAGAGCAGAGAAACCAGAGCTGCTTACCGTAATCTTGCCCCAGAGTTTGAAGAAGCAACCACCTGAGAGCCAGGAATTATTGTCAAAAGTCAAGAATGTGATAGAAAAGCCTCAAAATGATCATCTACCACTGATAGAAGCAAGCAGGTTGTGCAATATGGACATCATTTCACATGTACAGCAAGTTATATGCTTTGCGTTTCACGACAGTAGATTGCTCATGGAAACTTGTCAAGAGGCCAAAAATCTCCGCAAAATTGTGACTCTCTTCTACCTGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

289

Amino Acids

31.81

Weight (kDa)

7.11

Isoelectric Point (pI)

50.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 720
Acc65I GGTACC 1 cut(s) 308
AccB1I GGYRCC 1 cut(s) 308
AccII CGCG 2 cut(s) 263, 269
AciI CCGC 1 cut(s) 835
AclWI GGATC 1 cut(s) 355
AfaI GTAC 3 cut(s) 310, 556, 762
AfiI CCNNNNNNNGG 2 cut(s) 325, 466
AflIII ACRYGT 2 cut(s) 164, 757
AgsI TTSAA 3 cut(s) 103, 133, 634
AjnI CCWGG 3 cut(s) 324, 654, 858
AleI CACNNNNGTG 1 cut(s) 165
AluBI AGCT 3 cut(s) 504, 566, 604
AluI AGCT 3 cut(s) 504, 566, 604
Alw21I GWGCWC 1 cut(s) 172
AlwI GGATC 1 cut(s) 355
AoxI GGCC 2 cut(s) 431, 822
ApaI GGGCCC 1 cut(s) 435
ApeKI GCWGC 2 cut(s) 563, 604
Asp718I GGTACC 1 cut(s) 308
AspLEI GCGC 1 cut(s) 269
AspS9I GGNCC 3 cut(s) 431, 432, 456
AsuC2I CCSGG 1 cut(s) 460
AsuHPI GGTGA 2 cut(s) 31, 526
AvaII GGWCC 1 cut(s) 456
BaeGI GKGCMC 1 cut(s) 435
BanI GGYRCC 1 cut(s) 308
BanII GRGCYC 1 cut(s) 435
BbsI GAAGAC 1 cut(s) 264
Bbv12I GWGCWC 1 cut(s) 172
BbvI GCAGC 2 cut(s) 575, 591
BccI CCATC 1 cut(s) 505
BciT130I CCWGG 3 cut(s) 326, 656, 860
BclI TGATCA 3 cut(s) 229, 516, 703
BcnI CCSGG 1 cut(s) 460
BfmI CTRYAG 1 cut(s) 401
BfuAI ACCTGC 1 cut(s) 720
BisI GCNGC 2 cut(s) 564, 605
BlsI GCNGC 2 cut(s) 565, 606
Bme1390I CCNGG 4 cut(s) 326, 460, 656, 860
Bme18I GGWCC 1 cut(s) 456
BmgT120I GGNCC 3 cut(s) 431, 432, 456
BmiI GGNNCC 4 cut(s) 310, 433, 457, 458
BmrFI CCNGG 4 cut(s) 326, 460, 656, 860
BmsI GCATC 2 cut(s) 487, 557
BpiI GAAGAC 1 cut(s) 264
BpuEI CTTGAG 1 cut(s) 241
BpuMI CCSGG 1 cut(s) 460
BsaBI GATNNNNATC 1 cut(s) 515
BsaJI CCNNGG 1 cut(s) 324
BsaWI WCCGGW 1 cut(s) 551
Bsc4I CCNNNNNNNGG 2 cut(s) 325, 466
Bse1I ACTGG 1 cut(s) 277
Bse8I GATNNNNATC 1 cut(s) 515
BseBI CCWGG 3 cut(s) 326, 656, 860
BseDI CCNNGG 1 cut(s) 324
BseGI GGATG 3 cut(s) 182, 301, 548
BseJI GATNNNNATC 1 cut(s) 515
BseLI CCNNNNNNNGG 2 cut(s) 325, 466
BseMII CTCAG 3 cut(s) 93, 514, 639
BseNI ACTGG 1 cut(s) 277
BseRI GAGGAG 1 cut(s) 67
BseSI GKGCMC 1 cut(s) 435
BseXI GCAGC 2 cut(s) 575, 591
Bsh1236I CGCG 2 cut(s) 263, 269
BshFI GGCC 2 cut(s) 433, 824
BshNI GGYRCC 1 cut(s) 308
BsiHKAI GWGCWC 1 cut(s) 172
BsiSI CCGG 2 cut(s) 460, 552
BslFI GGGAC 1 cut(s) 469
BslI CCNNNNNNNGG 2 cut(s) 325, 466
BsmFI GGGAC 1 cut(s) 469
BsnI GGCC 2 cut(s) 433, 824
Bsp120I GGGCCC 1 cut(s) 431
Bsp1286I GDGCHC 2 cut(s) 172, 435
Bsp1407I TGTACA 1 cut(s) 760
Bsp143I GATC 5 cut(s) 229, 248, 347, 516, 703
BspACI CCGC 1 cut(s) 835
BspANI GGCC 2 cut(s) 433, 824
BspCNI CTCAG 3 cut(s) 92, 513, 640
BspFNI CGCG 2 cut(s) 263, 269
BspLI GGNNCC 4 cut(s) 310, 433, 457, 458
BspMI ACCTGC 1 cut(s) 720
BspPI GGATC 1 cut(s) 355
BspQI GCTCTTC 1 cut(s) 32
BspT107I GGYRCC 1 cut(s) 308
BsrGI TGTACA 1 cut(s) 760
BsrI ACTGG 1 cut(s) 277
BssECI CCNNGG 1 cut(s) 324
BssMI GATC 5 cut(s) 229, 248, 347, 516, 703
Bst2UI CCWGG 3 cut(s) 326, 656, 860
Bst4CI ACNGT 2 cut(s) 613, 794
Bst6I CTCTTC 2 cut(s) 32, 857
BstAPI GCANNNNNTGC 1 cut(s) 735
BstAUI TGTACA 1 cut(s) 760
BstC8I GCNNGC 3 cut(s) 121, 265, 727
BstDEI CTNAG 3 cut(s) 79, 500, 648
BstF5I GGATG 3 cut(s) 182, 301, 548
BstFNI CGCG 2 cut(s) 263, 269
BstHHI GCGC 1 cut(s) 269
BstKTI GATC 5 cut(s) 232, 251, 350, 519, 706
BstMBI GATC 5 cut(s) 229, 248, 347, 516, 703
BstMWI GCNNNNNNNGC 6 cut(s) 22, 31, 275, 430, 587, 735
BstNI CCWGG 3 cut(s) 326, 656, 860
BstNSI RCATGY 2 cut(s) 168, 761
BstSCI CCNGG 4 cut(s) 324, 458, 654, 858
BstSFI CTRYAG 1 cut(s) 401
BstSLI GKGCMC 1 cut(s) 435
BstUI CGCG 2 cut(s) 263, 269
BstV1I GCAGC 2 cut(s) 575, 591
BstV2I GAAGAC 1 cut(s) 264
BsuRI GGCC 2 cut(s) 433, 824
BtgZI GCGATG 1 cut(s) 524
BtsCI GGATG 3 cut(s) 182, 301, 548
BtsIMutI CAGTG 1 cut(s) 713
BveI ACCTGC 1 cut(s) 720
Cac8I GCNNGC 3 cut(s) 121, 265, 727
CfoI GCGC 1 cut(s) 269
Cfr13I GGNCC 3 cut(s) 431, 432, 456
CseI GACGC 2 cut(s) 225, 269
Csp6I GTAC 3 cut(s) 309, 555, 761
CviAII CATG 4 cut(s) 165, 475, 758, 805
CviQI GTAC 3 cut(s) 309, 555, 761
DdeI CTNAG 3 cut(s) 79, 500, 648
DpnI GATC 5 cut(s) 231, 250, 349, 518, 705
DpnII GATC 5 cut(s) 229, 248, 347, 516, 703
Eam1104I CTCTTC 2 cut(s) 32, 857
EarI CTCTTC 2 cut(s) 32, 857
Eco24I GRGCYC 1 cut(s) 435
Eco47I GGWCC 1 cut(s) 456
EcoO109I RGGNCCY 2 cut(s) 431, 456
EcoRII CCWGG 3 cut(s) 324, 654, 858
EcoT22I ATGCAT 1 cut(s) 480
EcoT38I GRGCYC 1 cut(s) 435
FaeI CATG 4 cut(s) 168, 478, 761, 808
FaqI GGGAC 1 cut(s) 469
FatI CATG 4 cut(s) 164, 474, 757, 804
FbaI TGATCA 3 cut(s) 229, 516, 703
Fnu4HI GCNGC 2 cut(s) 564, 605
FokI GGATG 3 cut(s) 169, 308, 535
FriOI GRGCYC 1 cut(s) 435
Fsp4HI GCNGC 2 cut(s) 564, 605
GlaI GCGC 1 cut(s) 268
GluI GCNGC 2 cut(s) 564, 605
HaeIII GGCC 2 cut(s) 433, 824
HapII CCGG 2 cut(s) 460, 552
HgaI GACGC 2 cut(s) 225, 269
HhaI GCGC 1 cut(s) 269
Hin1II CATG 4 cut(s) 168, 478, 761, 808
Hin6I GCGC 1 cut(s) 267
HinP1I GCGC 1 cut(s) 267
HinfI GANTC 3 cut(s) 471, 496, 847
HpaII CCGG 2 cut(s) 460, 552
HphI GGTGA 2 cut(s) 31, 526
Hpy166II GTNNAC 2 cut(s) 215, 385
Hpy188I TCNGA 3 cut(s) 82, 295, 370
Hpy188III TCNNGA 5 cut(s) 233, 482, 676, 788, 818
Hpy8I GTNNAC 2 cut(s) 215, 385
HpyAV CCTTC 3 cut(s) 109, 296, 338
HpyCH4III ACNGT 2 cut(s) 613, 794
HpyCH4V TGCA 6 cut(s) 16, 93, 119, 478, 563, 738
HpyF10VI GCNNNNNNNGC 6 cut(s) 22, 31, 275, 430, 587, 735
HpyF3I CTNAG 3 cut(s) 79, 500, 648
Hsp92II CATG 4 cut(s) 168, 478, 761, 808
HspAI GCGC 1 cut(s) 267
KflI GGGWCCC 1 cut(s) 456
KpnI GGTACC 1 cut(s) 312
Ksp22I TGATCA 3 cut(s) 229, 516, 703
Kzo9I GATC 5 cut(s) 229, 248, 347, 516, 703
LguI GCTCTTC 1 cut(s) 32
LmnI GCTCC 1 cut(s) 197
Lsp1109I GCAGC 2 cut(s) 575, 591
LweI GCATC 2 cut(s) 487, 557
MaeIII GTNAC 1 cut(s) 844
MalI GATC 5 cut(s) 231, 250, 349, 518, 705
MboI GATC 5 cut(s) 229, 248, 347, 516, 703
MhlI GDGCHC 2 cut(s) 172, 435
MluCI AATT 3 cut(s) 127, 659, 840
MlyI GAGTC 1 cut(s) 841
MnlI CCTC 9 cut(s) 15, 45, 52, 85, 88, 155, 299, 705, 814
Mph1103I ATGCAT 1 cut(s) 480
MseI TTAA 2 cut(s) 584, 865
MslI CAYNNNNRTG 1 cut(s) 165
MspA1I CMGCKG 1 cut(s) 566
MspI CCGG 2 cut(s) 460, 552
MspR9I CCNGG 4 cut(s) 326, 460, 656, 860
MvaI CCWGG 3 cut(s) 326, 656, 860
MvnI CGCG 2 cut(s) 263, 269
MwoI GCNNNNNNNGC 6 cut(s) 22, 31, 275, 430, 587, 735
NciI CCSGG 1 cut(s) 460
NdeII GATC 5 cut(s) 229, 248, 347, 516, 703
NlaIII CATG 4 cut(s) 168, 478, 761, 808
NlaIV GGNNCC 4 cut(s) 310, 433, 457, 458
NmuCI GTSAC 1 cut(s) 844
NsiI ATGCAT 1 cut(s) 480
NspI RCATGY 2 cut(s) 168, 761
OliI CACNNNNGTG 1 cut(s) 165
PciI ACATGT 2 cut(s) 164, 757
PciSI GCTCTTC 1 cut(s) 32
PfeI GAWTC 2 cut(s) 471, 496
PkrI GCNGC 2 cut(s) 565, 606
PleI GAGTC 1 cut(s) 841
PpsI GAGTC 1 cut(s) 841
PpuMI RGGWCCY 1 cut(s) 456
PscI ACATGT 2 cut(s) 164, 757
Psp5II RGGWCCY 1 cut(s) 456
Psp6I CCWGG 3 cut(s) 324, 654, 858
PspGI CCWGG 3 cut(s) 324, 654, 858
PspN4I GGNNCC 4 cut(s) 310, 433, 457, 458
PspOMI GGGCCC 1 cut(s) 431
PspPI GGNCC 3 cut(s) 431, 432, 456
PspPPI RGGWCCY 1 cut(s) 456
PvuII CAGCTG 1 cut(s) 566
RsaI GTAC 3 cut(s) 310, 556, 762
RsaNI GTAC 3 cut(s) 309, 555, 761
RseI CAYNNNNRTG 1 cut(s) 165
SapI GCTCTTC 1 cut(s) 32
SaqAI TTAA 2 cut(s) 584, 865
SatI GCNGC 2 cut(s) 564, 605
Sau3AI GATC 5 cut(s) 229, 248, 347, 516, 703
Sau96I GGNCC 3 cut(s) 431, 432, 456
SchI GAGTC 1 cut(s) 841
ScrFI CCNGG 4 cut(s) 326, 460, 656, 860
SduI GDGCHC 2 cut(s) 172, 435
SfaNI GCATC 2 cut(s) 487, 557
SfcI CTRYAG 1 cut(s) 401
SinI GGWCC 1 cut(s) 456
SmiMI CAYNNNNRTG 1 cut(s) 165
SmlI CTYRAG 1 cut(s) 220
SmoI CTYRAG 1 cut(s) 220
Sse9I AATT 3 cut(s) 127, 659, 840
SsiI CCGC 1 cut(s) 835
StyD4I CCNGG 4 cut(s) 324, 458, 654, 858
TaaI ACNGT 2 cut(s) 613, 794
TaqI TCGA 3 cut(s) 172, 247, 342
TasI AATT 3 cut(s) 127, 659, 840
TatI WGTACW 1 cut(s) 760
TfiI GAWTC 2 cut(s) 471, 496
Tru1I TTAA 2 cut(s) 584, 865
Tru9I TTAA 2 cut(s) 584, 865
TscAI CASTG 1 cut(s) 720
TseFI GTSAC 1 cut(s) 844
TseI GCWGC 2 cut(s) 563, 604
Tsp45I GTSAC 1 cut(s) 844
TspDTI ATGAA 3 cut(s) 270, 312, 463
TspRI CASTG 1 cut(s) 720
VpaK11BI GGWCC 1 cut(s) 456
XceI RCATGY 2 cut(s) 168, 761
Zsp2I ATGCAT 1 cut(s) 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.