pycom15g23880

May act early in the ethylene signal transduction pathway, possibly as an ethylene receptor, or as a regulator of the pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0003094
Physical Location & Seq
Forward (+)
18018989 .. 18023031
4043 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2232 bp
ATGTTGGCATGCAACTGTATTGAGCCACAATGGCCGGCTGATGAATTGCTGATGAAATATCAGTACATTTCAGATTTCTTTATTGCGCTTGCTTATTTCTCCATCCCACTAGAGTTGATCTACTTTGTCAAGAAGTCCGCAGTGTTTCCATACAGATGGGTCCTCGTACAATTTGGTGCTTTCATTGTACTGTGTGGGGCGACTCATCTTATCAACTTATGGACGTTCAGCATGCATTCAAGAACTGTGGCCATAGTAATGACCACGGCAAAGGTTTTAACTGCAGTGGTGTCATGCGCAACTGCCCTTATGCTTGTACATATTATACCTGATTTATTGAGTGTTAAAACTAGAGAACTATTCTTGAAGAACAAGGCTGCAGAGCTCGATAGAGAAATGGGCCTTATCCGCACACAGGAGGAAACAGGTCGACATGTCAGGATGTTGACCCATGAAATCAGAAGCACTCTTGATAGACATACTATACTTAAAACTACACTTGTCGAGTTAGGTAGAACTTTGGCATTGGAAGAGTGTGCCTTGTGGATGCCAACGCGTACTGGATTAGAGCTTCAACTATCCTATACACTCCGTCAGCAGAATCCAGTAGGATATACCGTACCCATTCATCTTCCTGTGATCAATCAAGTATTTAGTAGCAACCGTGCAGTAAAAATATCACCAAATTCTCCAGTGGCAAGACTGAGGCAACTTGCAGGAAGGCACATTCCTGGGGAAGTTGTTGCAGTTCGTGTGCCCCTTTTACATCTCTCTAACTTCCAAATTAATGACTGGCCTGAGCTCTCAACAAAACGCTATGCTTTGATGGTCTTGATGCTTCCCTCAGATAGTGCAAGGCAGTGGCACGTTCATGAGTTAGAATTGGTAGAAGTGGTTGCTGATCAGAAAGGTTGCGGTTGCTCTGTCACATGCTGCAATATTAGAAGAGTCAATGAGGGCAAGGGATCGATCCTTATGGAGCAGAATGTTGCGCTCGATTTAGCCAGGAGAGAAGCGGAGACTGCAATTCGTGCTCGCAATGATTTCTTGGCTGTTATGAACCATGAGATGAGAACTCCCATGCATGCAATTATTGCTCTTTCTTCTTTGCTACAGGAAACTGAGCTCACACCTGAGCAGCGTCTCATGGTTGAAACGATATTAAGGAGCAGCAACCTATTGGCTACCCTCGTAAATGATGTTTTGGATCTTTCAAGGCTTGAAGATGGCAGCCTTCAACTTGAAATAGCAACTTTTAACCTCCATTCCGTATTTAGAGAGGTTCATAATATGATTAAGCCTGTTGCATCTATCAAAAGGTTGTCAGTTACATTGAATATAGCTGCAGATTTGCCAATGTATGCCATAGGTGATGAAAAGCGTCTTATGCAAACTATTCTAAATGTTGTTGGGAATGCTGTGAAGTTCTCAAAAGAAGGGAGCATCTCAATCACAGGTTTTGTTGCAAAATCAGAATCTTTACGAGATTTCCGGGCTCCTGATTTCTTTCCAGTGCAAAGTGATAATCACTTTTATCTGCGCGTGCAGGTCAAAGATTCAGGATCTGGAATCAATCCTCAGGATATCCCAAAGCTATTTACCAAATTTGCACAAACTCAGACTTTAGCTACCCGAAATTCTGGTGGTAGTGGACTTGGCCTGGCAATTTGTAAGAGATTTGTAAATCTTATGGAAGGGCATATTTGGATTGAAAGTGAAGGTCTTGGCAAGGGATGCACAGCCACTTTCATTGTGAAACTCGGATTTCCAGAGCGTTCGAATGAGTCTAAGCTGCCTTTTGGACCCAAGCTGCAAGCAAATCATGTTCAAACTAACTTTCCAGGGTTGAAAGTCCTTGTCATGGATGATAATGGGGTCAGCAGGTCAGTGACAAAGGGACTCCTTGCGCACCTAGGATGTGATGTAACAGCTGTAAGCTTAATCGATGAGTCATTGCATGTTATTTCACAAGAGCACAAGGTGGTGTTCATGGATGTGAGCATGCCAGGAATCGATGGTTATGAACTTGCTGTCCGTATACATGAAAAGTTCACAAAACGTCATGAGCGGCCGGCATTAGTGGCACTTACCGGAAGCATAGACAAGATTACAAAGGAGAACTGCATGAGGGTTGGTGTGGATGGTGTTATACTAAAACCGGTTTCAGTTGATAAAATGAGGAGTGTTTTATCAGAAATATTAGAGCATCGAGTCCTATTCGAGGCCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000160 GO:0001101 GO:0002237 GO:0003674 GO:0003824 GO:0004672 GO:0004673 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006464 GO:0006468 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009308 GO:0009408 GO:0009593 GO:0009605 GO:0009607 GO:0009617 GO:0009625 GO:0009628 GO:0009651 GO:0009690 GO:0009719 GO:0009720 GO:0009723 GO:0009725 GO:0009726 GO:0009727 GO:0009733 GO:0009737 GO:0009739 GO:0009743 GO:0009755 GO:0009756 GO:0009873 GO:0009888 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010087 GO:0010104 GO:0010105 GO:0010119 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016775 GO:0016999 GO:0017000 GO:0017144 GO:0018106 GO:0018193 GO:0018202 GO:0019538 GO:0023051 GO:0023052 GO:0023057 GO:0032502 GO:0032870 GO:0033036 GO:0033037 GO:0033554 GO:0033993 GO:0034754 GO:0035556 GO:0036211 GO:0038023 GO:0038199 GO:0042221 GO:0042445 GO:0042545 GO:0042562 GO:0042742 GO:0042743 GO:0042802 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045229 GO:0046483 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0048856 GO:0050665 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051239 GO:0051301 GO:0051606 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0051740 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0060089 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070727 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071369 GO:0071495 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0072328 GO:0072593 GO:0097305 GO:0098542 GO:0140096 GO:1900140 GO:1901564 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:1903409 GO:2000026
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 map04016 map04075
Pfam Domains
Protein Families
GAF HATPase_c HisKA Response_reg

Protein Analysis

744

Amino Acids

83.1

Weight (kDa)

8.31

Isoelectric Point (pI)

44.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ETR1_N PF25487 17 - 115 1.2e-39 Ethylene receptor 1-like, N-terminal domain
GAF PF01590 158 - 302 5e-11 GAF domain
HisKA PF00512 345 - 410 2.4e-19 His Kinase A (phospho-acceptor) domain
HATPase_c PF02518 457 - 587 1.7e-24 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Response_reg PF00072 618 - 729 6.8e-16 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 298, 1908
Acc36I ACCTGC 2 cut(s) 1537, 1872
AccBSI CCGCTC 1 cut(s) 2068
AccI GTMKAC 2 cut(s) 430, 2038
AccII CGCG 2 cut(s) 556, 1542
AciI CCGC 5 cut(s) 138, 409, 915, 1016, 2068
AclWI GGATC 4 cut(s) 964, 973, 1215, 1570
AcoI YGGCCR 3 cut(s) 32, 249, 2069
AcsI RAATTY 3 cut(s) 685, 1604, 1636
AdeI CACNNNGTG 1 cut(s) 1981
AfaI GTAC 6 cut(s) 65, 168, 189, 318, 559, 621
AfiI CCNNNNNNNGG 3 cut(s) 415, 1861, 2221
AflIII ACRYGT 2 cut(s) 433, 554
AgeI ACCGGT 1 cut(s) 2158
AjnI CCWGG 5 cut(s) 730, 1004, 1659, 1840, 2005
AloI GAACNNNNNNTCC 2 cut(s) 2016, 2048
Alw21I GWGCWC 5 cut(s) 387, 804, 1036, 1128, 1977
Alw26I GTCTC 2 cut(s) 1013, 1148
AlwI GGATC 4 cut(s) 964, 973, 1215, 1570
AlwNI CAGNNNCTG 1 cut(s) 1565
AoxI GGCC 7 cut(s) 32, 249, 400, 794, 1657, 2069, 2223
ApeKI GCWGC 8 cut(s) 377, 933, 1138, 1170, 1230, 1343, 1792, 1810
ApoI RAATTY 3 cut(s) 685, 1604, 1636
AseI ATTAAT 1 cut(s) 786
AsiGI ACCGGT 1 cut(s) 2158
AspA2I CCTAGG 1 cut(s) 1912
AspLEI GCGC 5 cut(s) 88, 299, 994, 1542, 1909
AspS9I GGNCC 3 cut(s) 160, 400, 1802
AsuC2I CCSGG 1 cut(s) 1493
AsuHPI GGTGA 2 cut(s) 672, 1382
AsuII TTCGAA 1 cut(s) 1778
AvaII GGWCC 2 cut(s) 160, 1802
AvrII CCTAGG 1 cut(s) 1912
AxyI CCTNAGG 1 cut(s) 1578
BaeGI GKGCMC 1 cut(s) 759
BalI TGGCCA 1 cut(s) 251
BanII GRGCYC 4 cut(s) 387, 804, 1128, 1498
Bbv12I GWGCWC 5 cut(s) 387, 804, 1036, 1128, 1977
BbvI GCAGC 8 cut(s) 364, 920, 1150, 1182, 1242, 1330, 1779, 1797
BccI CCATC 6 cut(s) 110, 150, 820, 1220, 2009, 2135
BceAI ACGGC 1 cut(s) 282
BciT130I CCWGG 5 cut(s) 732, 1006, 1661, 1842, 2007
BclI TGATCA 2 cut(s) 639, 901
BcnI CCSGG 1 cut(s) 1493
BcoDI GTCTC 2 cut(s) 1013, 1148
BfaI CTAG 3 cut(s) 110, 351, 1913
BfmI CTRYAG 4 cut(s) 282, 378, 1112, 1344
BfuAI ACCTGC 2 cut(s) 1537, 1872
BglI GCCNNNNNGGC 1 cut(s) 31
BisI GCNGC 9 cut(s) 378, 934, 1139, 1171, 1231, 1344, 1793, 1811, 2069
BlnI CCTAGG 1 cut(s) 1912
BlsI GCNGC 9 cut(s) 379, 935, 1140, 1172, 1232, 1345, 1794, 1812, 2070
Bme1390I CCNGG 6 cut(s) 732, 1006, 1493, 1661, 1842, 2007
Bme18I GGWCC 2 cut(s) 160, 1802
BmgT120I GGNCC 3 cut(s) 160, 400, 1802
BmiI GGNNCC 3 cut(s) 161, 1497, 1804
BmrFI CCNGG 6 cut(s) 732, 1006, 1493, 1661, 1842, 2007
BmsI GCATC 6 cut(s) 537, 825, 1316, 1452, 1724, 2215
BpmI CTGGAG 1 cut(s) 675
Bpu10I CCTNAGC 2 cut(s) 798, 1134
Bpu14I TTCGAA 1 cut(s) 1778
BpuMI CCSGG 1 cut(s) 1493
Bsa29I ATCGAT 3 cut(s) 968, 1944, 2013
BsaJI CCNNGG 4 cut(s) 264, 731, 1841, 1912
BsaWI WCCGGW 2 cut(s) 2090, 2158
Bsc4I CCNNNNNNNGG 3 cut(s) 415, 1861, 2221
Bse118I RCCGGY 3 cut(s) 34, 2071, 2158
Bse1I ACTGG 5 cut(s) 565, 605, 692, 797, 1511
Bse21I CCTNAGG 1 cut(s) 1578
Bse3DI GCAATG 2 cut(s) 1045, 1952
BseBI CCWGG 5 cut(s) 732, 1006, 1661, 1842, 2007
BseCI ATCGAT 3 cut(s) 968, 1944, 2013
BseDI CCNNGG 4 cut(s) 264, 731, 1841, 1912
BseGI GGATG 8 cut(s) 102, 447, 552, 1739, 1870, 1922, 1999, 2146
BseLI CCNNNNNNNGG 3 cut(s) 415, 1861, 2221
BseMI GCAATG 2 cut(s) 1045, 1952
BseMII CTCAG 7 cut(s) 695, 789, 858, 1113, 1125, 1592, 1631
BseNI ACTGG 5 cut(s) 565, 605, 692, 797, 1511
BseRI GAGGAG 1 cut(s) 2194
BseSI GKGCMC 1 cut(s) 759
BseX3I CGGCCG 1 cut(s) 2069
BseXI GCAGC 8 cut(s) 364, 920, 1150, 1182, 1242, 1330, 1779, 1797
BsgI GTGCAG 2 cut(s) 687, 1565
Bsh1236I CGCG 2 cut(s) 556, 1542
Bsh1285I CGRYCG 1 cut(s) 2072
BshFI GGCC 7 cut(s) 34, 251, 402, 796, 1659, 2071, 2225
BshTI ACCGGT 1 cut(s) 2158
BshVI ATCGAT 3 cut(s) 968, 1944, 2013
BsiEI CGRYCG 1 cut(s) 2072
BsiHKAI GWGCWC 5 cut(s) 387, 804, 1036, 1128, 1977
BsiSI CCGG 5 cut(s) 35, 1492, 2072, 2091, 2159
BslFI GGGAC 1 cut(s) 1911
BslI CCNNNNNNNGG 3 cut(s) 415, 1861, 2221
BsmAI GTCTC 2 cut(s) 1013, 1148
BsmBI CGTCTC 1 cut(s) 1148
BsmFI GGGAC 1 cut(s) 1911
BsmI GAATGC 2 cut(s) 235, 1420
BsnI GGCC 7 cut(s) 34, 251, 402, 796, 1659, 2071, 2225
Bsp119I TTCGAA 1 cut(s) 1778
Bsp1286I GDGCHC 7 cut(s) 387, 759, 804, 1036, 1128, 1498, 1977
Bsp1407I TGTACA 1 cut(s) 316
Bsp143I GATC 7 cut(s) 117, 639, 901, 965, 969, 1207, 1562
BspACI CCGC 5 cut(s) 138, 409, 915, 1016, 2068
BspANI GGCC 7 cut(s) 34, 251, 402, 796, 1659, 2071, 2225
BspCNI CTCAG 7 cut(s) 696, 790, 857, 1114, 1126, 1591, 1630
BspDI ATCGAT 3 cut(s) 968, 1944, 2013
BspFNI CGCG 2 cut(s) 556, 1542
BspHI TCATGA 2 cut(s) 871, 2062
BspLI GGNNCC 3 cut(s) 161, 1497, 1804
BspMAI CTGCAG 3 cut(s) 286, 382, 1348
BspMI ACCTGC 2 cut(s) 1537, 1872
BspPI GGATC 4 cut(s) 964, 973, 1215, 1570
BspT104I TTCGAA 1 cut(s) 1778
BsrBI CCGCTC 1 cut(s) 2068
BsrDI GCAATG 2 cut(s) 1045, 1952
BsrFI RCCGGY 3 cut(s) 34, 2071, 2158
BsrGI TGTACA 1 cut(s) 316
BsrI ACTGG 5 cut(s) 565, 605, 692, 797, 1511
BssAI RCCGGY 3 cut(s) 34, 2071, 2158
BssECI CCNNGG 4 cut(s) 264, 731, 1841, 1912
BssMI GATC 7 cut(s) 117, 639, 901, 965, 969, 1207, 1562
BssNAI GTATAC 1 cut(s) 2039
BssT1I CCWWGG 1 cut(s) 1912
Bst1107I GTATAC 1 cut(s) 2039
Bst2UI CCWGG 5 cut(s) 732, 1006, 1661, 1842, 2007
Bst4CI ACNGT 5 cut(s) 17, 192, 247, 619, 665
Bst6I CTCTTC 2 cut(s) 525, 940
BstAPI GCANNNNNTGC 3 cut(s) 1031, 1094, 1734
BstAUI TGTACA 1 cut(s) 316
BstBI TTCGAA 1 cut(s) 1778
BstDEI CTNAG 8 cut(s) 704, 798, 844, 1122, 1134, 1578, 1617, 1788
BstDSI CCRYGG 1 cut(s) 264
BstENI CCTNNNNNAGG 1 cut(s) 2219
BstF5I GGATG 8 cut(s) 102, 447, 552, 1739, 1870, 1922, 1999, 2146
BstFNI CGCG 2 cut(s) 556, 1542
BstHHI GCGC 5 cut(s) 88, 299, 994, 1542, 1909
BstKTI GATC 7 cut(s) 120, 642, 904, 968, 972, 1210, 1565
BstMAI GTCTC 2 cut(s) 1013, 1148
BstMBI GATC 7 cut(s) 117, 639, 901, 965, 969, 1207, 1562
BstMCI CGRYCG 1 cut(s) 2072
BstMWI GCNNNNNNNGC 8 cut(s) 31, 408, 1022, 1031, 1094, 1387, 1734, 2081
BstNI CCWGG 5 cut(s) 732, 1006, 1661, 1842, 2007
BstNSI RCATGY 7 cut(s) 12, 235, 437, 933, 1088, 1961, 2005
BstSCI CCNGG 6 cut(s) 730, 1004, 1491, 1659, 1840, 2005
BstSFI CTRYAG 4 cut(s) 282, 378, 1112, 1344
BstSLI GKGCMC 1 cut(s) 759
BstUI CGCG 2 cut(s) 556, 1542
BstV1I GCAGC 8 cut(s) 364, 920, 1150, 1182, 1242, 1330, 1779, 1797
BstX2I RGATCY 2 cut(s) 1207, 1562
BstXI CCANNNNNNTGG 1 cut(s) 156
BstYI RGATCY 2 cut(s) 1207, 1562
BstZ17I GTATAC 1 cut(s) 2039
BstZI CGGCCG 1 cut(s) 2069
Bsu15I ATCGAT 3 cut(s) 968, 1944, 2013
Bsu36I CCTNAGG 1 cut(s) 1578
BsuRI GGCC 7 cut(s) 34, 251, 402, 796, 1659, 2071, 2225
BsuTUI ATCGAT 3 cut(s) 968, 1944, 2013
BtgI CCRYGG 1 cut(s) 264
BtsCI GGATG 8 cut(s) 102, 447, 552, 1739, 1870, 1922, 1999, 2146
BtsI GCAGTG 3 cut(s) 147, 291, 866
BtsIMutI CAGTG 6 cut(s) 147, 291, 699, 866, 1518, 1893
BveI ACCTGC 2 cut(s) 1537, 1872
CaiI CAGNNNCTG 1 cut(s) 1565
CciI TCATGA 2 cut(s) 871, 2062
CfoI GCGC 5 cut(s) 88, 299, 994, 1542, 1909
Cfr10I RCCGGY 3 cut(s) 34, 2071, 2158
Cfr13I GGNCC 3 cut(s) 160, 400, 1802
ClaI ATCGAT 3 cut(s) 968, 1944, 2013
CseI GACGC 2 cut(s) 1130, 1370
Csp6I GTAC 6 cut(s) 64, 167, 188, 317, 558, 620
CspAI ACCGGT 1 cut(s) 2158
CspCI CAANNNNNGTGG 6 cut(s) 96, 131, 228, 263, 1732, 1767
CviQI GTAC 6 cut(s) 64, 167, 188, 317, 558, 620
DdeI CTNAG 8 cut(s) 704, 798, 844, 1122, 1134, 1578, 1617, 1788
DpnI GATC 7 cut(s) 119, 641, 903, 967, 971, 1209, 1564
DpnII GATC 7 cut(s) 117, 639, 901, 965, 969, 1207, 1562
DraIII CACNNNGTG 1 cut(s) 1981
EaeI YGGCCR 3 cut(s) 32, 249, 2069
EagI CGGCCG 1 cut(s) 2069
Eam1104I CTCTTC 2 cut(s) 525, 940
EarI CTCTTC 2 cut(s) 525, 940
Ecl136II GAGCTC 3 cut(s) 385, 802, 1126
EclXI CGGCCG 1 cut(s) 2069
Eco130I CCWWGG 1 cut(s) 1912
Eco24I GRGCYC 4 cut(s) 387, 804, 1128, 1498
Eco32I GATATC 1 cut(s) 1585
Eco47I GGWCC 2 cut(s) 160, 1802
Eco52I CGGCCG 1 cut(s) 2069
Eco53kI GAGCTC 3 cut(s) 385, 802, 1126
Eco81I CCTNAGG 1 cut(s) 1578
EcoICRI GAGCTC 3 cut(s) 385, 802, 1126
EcoNI CCTNNNNNAGG 1 cut(s) 2219
EcoO109I RGGNCCY 1 cut(s) 160
EcoRII CCWGG 5 cut(s) 730, 1004, 1659, 1840, 2005
EcoRV GATATC 1 cut(s) 1585
EcoT14I CCWWGG 1 cut(s) 1912
EcoT22I ATGCAT 2 cut(s) 237, 1086
EcoT38I GRGCYC 4 cut(s) 387, 804, 1128, 1498
ErhI CCWWGG 1 cut(s) 1912
Esp3I CGTCTC 1 cut(s) 1148
FaqI GGGAC 1 cut(s) 1911
FbaI TGATCA 2 cut(s) 639, 901
FblI GTMKAC 2 cut(s) 430, 2038
Fnu4HI GCNGC 9 cut(s) 378, 934, 1139, 1171, 1231, 1344, 1793, 1811, 2069
FokI GGATG 8 cut(s) 89, 454, 559, 1746, 1877, 1929, 2006, 2153
FriOI GRGCYC 4 cut(s) 387, 804, 1128, 1498
Fsp4HI GCNGC 9 cut(s) 378, 934, 1139, 1171, 1231, 1344, 1793, 1811, 2069
FspBI CTAG 3 cut(s) 110, 351, 1913
FspI TGCGCA 2 cut(s) 298, 1908
GlaI GCGC 5 cut(s) 87, 298, 993, 1541, 1908
GluI GCNGC 9 cut(s) 378, 934, 1139, 1171, 1231, 1344, 1793, 1811, 2069
GsuI CTGGAG 1 cut(s) 675
HaeIII GGCC 7 cut(s) 34, 251, 402, 796, 1659, 2071, 2225
HapII CCGG 5 cut(s) 35, 1492, 2072, 2091, 2159
HgaI GACGC 2 cut(s) 1130, 1370
HhaI GCGC 5 cut(s) 88, 299, 994, 1542, 1909
Hin6I GCGC 5 cut(s) 86, 297, 992, 1540, 1907
HinP1I GCGC 5 cut(s) 86, 297, 992, 1540, 1907
HincII GTYRAC 2 cut(s) 431, 447
HindII GTYRAC 2 cut(s) 431, 447
HindIII AAGCTT 1 cut(s) 1936
HpaII CCGG 5 cut(s) 35, 1492, 2072, 2091, 2159
HphI GGTGA 2 cut(s) 672, 1382
Hpy166II GTNNAC 5 cut(s) 431, 447, 1652, 2039, 2052
Hpy188I TCNGA 8 cut(s) 73, 461, 847, 906, 1474, 1620, 1763, 2194
Hpy8I GTNNAC 5 cut(s) 431, 447, 1652, 2039, 2052
HpyAV CCTTC 5 cut(s) 714, 1244, 1430, 1688, 1712
HpyCH4III ACNGT 5 cut(s) 17, 192, 247, 619, 665
HpyCH4IV ACGT 3 cut(s) 224, 867, 2059
HpyF10VI GCNNNNNNNGC 8 cut(s) 31, 408, 1022, 1031, 1094, 1387, 1734, 2081
HpyF3I CTNAG 8 cut(s) 704, 798, 844, 1122, 1134, 1578, 1617, 1788
HpySE526I ACGT 3 cut(s) 224, 867, 2059
HspAI GCGC 5 cut(s) 86, 297, 992, 1540, 1907
KroI GCCGGC 2 cut(s) 34, 2071
KroNI GCCGGC 2 cut(s) 36, 2073
Ksp22I TGATCA 2 cut(s) 639, 901
Kzo9I GATC 7 cut(s) 117, 639, 901, 965, 969, 1207, 1562
LmnI GCTCC 4 cut(s) 979, 1167, 1440, 1501
Lsp1109I GCAGC 8 cut(s) 364, 920, 1150, 1182, 1242, 1330, 1779, 1797
LweI GCATC 6 cut(s) 537, 825, 1316, 1452, 1724, 2215
MaeI CTAG 3 cut(s) 110, 351, 1913
MaeII ACGT 3 cut(s) 224, 867, 2059
MaeIII GTNAC 4 cut(s) 925, 1327, 1888, 1924
MalI GATC 7 cut(s) 119, 641, 903, 967, 971, 1209, 1564
MbiI CCGCTC 1 cut(s) 2068
MboI GATC 7 cut(s) 117, 639, 901, 965, 969, 1207, 1562
MboII GAAGA 6 cut(s) 379, 542, 623, 957, 1095, 1235
MflI RGATCY 2 cut(s) 1207, 1562
MhlI GDGCHC 7 cut(s) 387, 759, 804, 1036, 1128, 1498, 1977
MlsI TGGCCA 1 cut(s) 251
MluI ACGCGT 1 cut(s) 554
MluNI TGGCCA 1 cut(s) 251
MlyI GAGTC 6 cut(s) 196, 957, 1793, 1893, 1958, 2220
Mox20I TGGCCA 1 cut(s) 251
Mph1103I ATGCAT 2 cut(s) 237, 1086
MroNI GCCGGC 2 cut(s) 34, 2071
MscI TGGCCA 1 cut(s) 251
MseI TTAA 8 cut(s) 278, 345, 489, 786, 1163, 1257, 1296, 1940
MslI CAYNNNNRTG 4 cut(s) 154, 257, 870, 1994
Msp20I TGGCCA 1 cut(s) 251
MspA1I CMGCKG 1 cut(s) 1931
MspI CCGG 5 cut(s) 35, 1492, 2072, 2091, 2159
MspR9I CCNGG 6 cut(s) 732, 1006, 1493, 1661, 1842, 2007
Mva1269I GAATGC 2 cut(s) 235, 1420
MvaI CCWGG 5 cut(s) 732, 1006, 1661, 1842, 2007
MvnI CGCG 2 cut(s) 556, 1542
MwoI GCNNNNNNNGC 8 cut(s) 31, 408, 1022, 1031, 1094, 1387, 1734, 2081
NaeI GCCGGC 2 cut(s) 36, 2073
NciI CCSGG 1 cut(s) 1493
NdeII GATC 7 cut(s) 117, 639, 901, 965, 969, 1207, 1562
NgoMIV GCCGGC 2 cut(s) 34, 2071
NlaIV GGNNCC 3 cut(s) 161, 1497, 1804
NmuCI GTSAC 2 cut(s) 925, 1888
NsbI TGCGCA 2 cut(s) 298, 1908
NsiI ATGCAT 2 cut(s) 237, 1086
NspI RCATGY 7 cut(s) 12, 235, 437, 933, 1088, 1961, 2005
NspV TTCGAA 1 cut(s) 1778
PaeI GCATGC 4 cut(s) 12, 235, 1088, 2005
PagI TCATGA 2 cut(s) 871, 2062
PciI ACATGT 1 cut(s) 433
PctI GAATGC 2 cut(s) 235, 1420
PdiI GCCGGC 2 cut(s) 36, 2073
PfeI GAWTC 5 cut(s) 601, 1475, 1556, 1569, 2010
PinAI ACCGGT 1 cut(s) 2158
PkrI GCNGC 9 cut(s) 379, 935, 1140, 1172, 1232, 1345, 1794, 1812, 2070
PleI GAGTC 6 cut(s) 196, 956, 1792, 1893, 1957, 2219
PpsI GAGTC 6 cut(s) 196, 956, 1792, 1893, 1957, 2219
PpuMI RGGWCCY 1 cut(s) 160
PscI ACATGT 1 cut(s) 433
PshBI ATTAAT 1 cut(s) 786
Psp124BI GAGCTC 3 cut(s) 387, 804, 1128
Psp5II RGGWCCY 1 cut(s) 160
Psp6I CCWGG 5 cut(s) 730, 1004, 1659, 1840, 2005
PspGI CCWGG 5 cut(s) 730, 1004, 1659, 1840, 2005
PspN4I GGNNCC 3 cut(s) 161, 1497, 1804
PspPI GGNCC 3 cut(s) 160, 400, 1802
PspPPI RGGWCCY 1 cut(s) 160
PstI CTGCAG 3 cut(s) 286, 382, 1348
PstNI CAGNNNCTG 1 cut(s) 1565
PsuI RGATCY 2 cut(s) 1207, 1562
PvuII CAGCTG 1 cut(s) 1931
RsaI GTAC 6 cut(s) 65, 168, 189, 318, 559, 621
RsaNI GTAC 6 cut(s) 64, 167, 188, 317, 558, 620
RseI CAYNNNNRTG 4 cut(s) 154, 257, 870, 1994
SacI GAGCTC 3 cut(s) 387, 804, 1128
SalI GTCGAC 1 cut(s) 429
SaqAI TTAA 8 cut(s) 278, 345, 489, 786, 1163, 1257, 1296, 1940
SatI GCNGC 9 cut(s) 378, 934, 1139, 1171, 1231, 1344, 1793, 1811, 2069
Sau3AI GATC 7 cut(s) 117, 639, 901, 965, 969, 1207, 1562
Sau96I GGNCC 3 cut(s) 160, 400, 1802
SchI GAGTC 6 cut(s) 196, 957, 1793, 1893, 1958, 2220
ScrFI CCNGG 6 cut(s) 732, 1006, 1493, 1661, 1842, 2007
SduI GDGCHC 7 cut(s) 387, 759, 804, 1036, 1128, 1498, 1977
SfaNI GCATC 6 cut(s) 537, 825, 1316, 1452, 1724, 2215
SfcI CTRYAG 4 cut(s) 282, 378, 1112, 1344
SfuI TTCGAA 1 cut(s) 1778
SinI GGWCC 2 cut(s) 160, 1802
SmiMI CAYNNNNRTG 4 cut(s) 154, 257, 870, 1994
SphI GCATGC 4 cut(s) 12, 235, 1088, 2005
SsiI CCGC 5 cut(s) 138, 409, 915, 1016, 2068
SspI AATATT 2 cut(s) 940, 2199
SspMI CTAG 3 cut(s) 110, 351, 1913
SstI GAGCTC 3 cut(s) 387, 804, 1128
StyD4I CCNGG 6 cut(s) 730, 1004, 1491, 1659, 1840, 2005
StyI CCWWGG 1 cut(s) 1912
TaaI ACNGT 5 cut(s) 17, 192, 247, 619, 665
TaiI ACGT 3 cut(s) 227, 870, 2062
TatI WGTACW 3 cut(s) 63, 187, 316
TauI GCSGC 1 cut(s) 2071
TfiI GAWTC 5 cut(s) 601, 1475, 1556, 1569, 2010
Tru1I TTAA 8 cut(s) 278, 345, 489, 786, 1163, 1257, 1296, 1940
Tru9I TTAA 8 cut(s) 278, 345, 489, 786, 1163, 1257, 1296, 1940
TscAI CASTG 6 cut(s) 147, 291, 699, 866, 1518, 1893
TseFI GTSAC 2 cut(s) 925, 1888
TseI GCWGC 8 cut(s) 377, 933, 1138, 1170, 1230, 1343, 1792, 1810
Tsp45I GTSAC 2 cut(s) 925, 1888
TspGWI ACGGA 3 cut(s) 581, 1258, 2024
TspRI CASTG 6 cut(s) 147, 291, 699, 866, 1518, 1893
VpaK11BI GGWCC 2 cut(s) 160, 1802
VspI ATTAAT 1 cut(s) 786
XagI CCTNNNNNAGG 1 cut(s) 2219
XapI RAATTY 3 cut(s) 685, 1604, 1636
XceI RCATGY 7 cut(s) 12, 235, 437, 933, 1088, 1961, 2005
XmaJI CCTAGG 1 cut(s) 1912
XmiI GTMKAC 2 cut(s) 430, 2038
XspI CTAG 3 cut(s) 110, 351, 1913
Zsp2I ATGCAT 2 cut(s) 237, 1086
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.