pycom15g36090
MADS Family

Agamous-like MADS-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0003722
Physical Location & Seq
Forward (+)
36182278 .. 36187342
5065 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 675 bp
ATGGGGAGGGGAAAGATTGAAATCAAGAGGATTGAGAACACGACGAATCGGCAAGTGACCTTCTGCAAACGAAGAAATGGGCTGCTGAAGAAAGCATATGAATTATCCATTCTCTGTGATGCTGAAATTGCCCTCATTGTCTTCTCTAGCCGGGGCCGTCTCTACGAGTACTCTAACAACAACAGTATAAGAAACACCATAGAGAGGTACAAGAAGGCATGTTCAGATAGCACAGGATCAACTTCTATTACTGAAATTAACGCTCAGTATTATCAACAGGAATCGGCAAAACTGAGGCAACAGATTCAAATGCTGCAGAACTCTAACAGGCACTTAATGGGAGATGCCTTGAGTAATCTAACTGTGAAAGAACTAAAGCAGCTGGAGAATAAGCTTGAACGAGGCATGACTAGAATCAGGTCCAAGAAGGAAGAAATGCTGATTGCAGAGATTGAGTACTTGCAGAAAAAGGAGATTGAGCTGGAAAACGAAAATGTTTATCTTCGAACTAAGATATCAGAAGTTGAGAGGCTTCACCAAGCAAACATGGTTTCTGTGCCAGAGATGAATGCAATCCAGGCATTAGCTTCTCGCAATTTCTTTAGCCAGAATATCATTGAGGGTGGAGGAGCTACCTTCCCACAGCAAAACAAGAAGATTCTCCATCTTGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
K-box SRF-TF

Protein Analysis

225

Amino Acids

25.85

Weight (kDa)

9.45

Isoelectric Point (pI)

58.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 10 - 57 1.7e-26 SRF-type transcription factor (DNA-binding and dimerisation domain)
K-box PF01486 87 - 172 2.8e-28 K-box region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 244
AcuI CTGAAG 1 cut(s) 107
AfaI GTAC 3 cut(s) 170, 209, 458
AfiI CCNNNNNNNGG 1 cut(s) 204
AgsI TTSAA 3 cut(s) 20, 308, 398
AjnI CCWGG 1 cut(s) 576
AluBI AGCT 5 cut(s) 382, 394, 481, 587, 632
AluI AGCT 5 cut(s) 382, 394, 481, 587, 632
Alw26I GTCTC 1 cut(s) 164
AlwI GGATC 1 cut(s) 244
AoxI GGCC 1 cut(s) 154
ApeKI GCWGC 3 cut(s) 82, 313, 379
AspS9I GGNCC 2 cut(s) 154, 420
AsuC2I CCSGG 1 cut(s) 152
AsuHPI GGTGA 1 cut(s) 527
AsuII TTCGAA 1 cut(s) 505
AvaII GGWCC 1 cut(s) 420
BbsI GAAGAC 1 cut(s) 133
BbvI GCAGC 3 cut(s) 69, 300, 391
BccI CCATC 1 cut(s) 672
BceAI ACGGC 1 cut(s) 141
BciT130I CCWGG 1 cut(s) 578
BcnI CCSGG 1 cut(s) 152
BcoDI GTCTC 1 cut(s) 164
BfaI CTAG 2 cut(s) 147, 411
BfmI CTRYAG 1 cut(s) 314
BisI GCNGC 3 cut(s) 83, 314, 380
BlsI GCNGC 3 cut(s) 84, 315, 381
BmcAI AGTACT 2 cut(s) 170, 458
Bme1390I CCNGG 2 cut(s) 152, 578
Bme18I GGWCC 1 cut(s) 420
BmgT120I GGNCC 2 cut(s) 154, 420
BmiI GGNNCC 1 cut(s) 155
BmrFI CCNGG 2 cut(s) 152, 578
BmsI GCATC 2 cut(s) 109, 334
BpiI GAAGAC 1 cut(s) 133
BpmI CTGGAG 1 cut(s) 404
Bpu14I TTCGAA 1 cut(s) 505
BpuEI CTTGAG 1 cut(s) 370
BpuMI CCSGG 1 cut(s) 152
BsaBI GATNNNNATC 1 cut(s) 20
BsaJI CCNNGG 1 cut(s) 151
Bsc4I CCNNNNNNNGG 1 cut(s) 204
Bse8I GATNNNNATC 1 cut(s) 20
BseBI CCWGG 1 cut(s) 578
BseDI CCNNGG 1 cut(s) 151
BseJI GATNNNNATC 1 cut(s) 20
BseLI CCNNNNNNNGG 1 cut(s) 204
BseMII CTCAG 2 cut(s) 278, 284
BseRI GAGGAG 1 cut(s) 642
BseXI GCAGC 3 cut(s) 69, 300, 391
BshFI GGCC 1 cut(s) 156
BsiSI CCGG 1 cut(s) 151
BslI CCNNNNNNNGG 1 cut(s) 204
BsmAI GTCTC 1 cut(s) 164
BsmBI CGTCTC 1 cut(s) 164
BsmI GAATGC 1 cut(s) 574
BsnI GGCC 1 cut(s) 156
Bsp119I TTCGAA 1 cut(s) 505
Bsp143I GATC 1 cut(s) 236
BspANI GGCC 1 cut(s) 156
BspCNI CTCAG 2 cut(s) 277, 285
BspLI GGNNCC 1 cut(s) 155
BspMAI CTGCAG 1 cut(s) 318
BspPI GGATC 1 cut(s) 244
BspT104I TTCGAA 1 cut(s) 505
BssECI CCNNGG 1 cut(s) 151
BssMI GATC 1 cut(s) 236
Bst2UI CCWGG 1 cut(s) 578
Bst4CI ACNGT 2 cut(s) 185, 364
BstBI TTCGAA 1 cut(s) 505
BstDEI CTNAG 3 cut(s) 264, 293, 510
BstKTI GATC 1 cut(s) 239
BstMAI GTCTC 1 cut(s) 164
BstMBI GATC 1 cut(s) 236
BstMWI GCNNNNNNNGC 2 cut(s) 128, 578
BstNI CCWGG 1 cut(s) 578
BstNSI RCATGY 1 cut(s) 222
BstSCI CCNGG 2 cut(s) 150, 576
BstSFI CTRYAG 1 cut(s) 314
BstV1I GCAGC 3 cut(s) 69, 300, 391
BstV2I GAAGAC 1 cut(s) 133
BsuRI GGCC 1 cut(s) 156
Cfr13I GGNCC 2 cut(s) 154, 420
Csp6I GTAC 3 cut(s) 169, 208, 457
CviAII CATG 3 cut(s) 219, 406, 547
CviQI GTAC 3 cut(s) 169, 208, 457
DdeI CTNAG 3 cut(s) 264, 293, 510
DpnI GATC 1 cut(s) 238
DpnII GATC 1 cut(s) 236
Eco32I GATATC 1 cut(s) 516
Eco47I GGWCC 1 cut(s) 420
Eco57I CTGAAG 1 cut(s) 107
EcoRII CCWGG 1 cut(s) 576
EcoRV GATATC 1 cut(s) 516
Esp3I CGTCTC 1 cut(s) 164
FaeI CATG 3 cut(s) 222, 409, 550
FaiI YATR 7 cut(s) 97, 99, 188, 200, 220, 407, 548
FatI CATG 3 cut(s) 218, 405, 546
FauNDI CATATG 1 cut(s) 97
Fnu4HI GCNGC 3 cut(s) 83, 314, 380
Fsp4HI GCNGC 3 cut(s) 83, 314, 380
FspBI CTAG 2 cut(s) 147, 411
GluI GCNGC 3 cut(s) 83, 314, 380
GsuI CTGGAG 1 cut(s) 404
HaeIII GGCC 1 cut(s) 156
HapII CCGG 1 cut(s) 151
Hin1II CATG 3 cut(s) 222, 409, 550
HindIII AAGCTT 1 cut(s) 392
HinfI GANTC 5 cut(s) 46, 281, 304, 414, 658
HpaII CCGG 1 cut(s) 151
HphI GGTGA 1 cut(s) 527
Hpy188I TCNGA 2 cut(s) 226, 520
Hpy188III TCNNGA 1 cut(s) 25
Hpy99I CGWCG 1 cut(s) 46
HpyAV CCTTC 4 cut(s) 70, 208, 421, 646
HpyCH4III ACNGT 2 cut(s) 185, 364
HpyCH4V TGCA 5 cut(s) 66, 316, 446, 463, 572
HpyF10VI GCNNNNNNNGC 2 cut(s) 128, 578
HpyF3I CTNAG 3 cut(s) 264, 293, 510
Hsp92II CATG 3 cut(s) 222, 409, 550
Kzo9I GATC 1 cut(s) 236
LmnI GCTCC 1 cut(s) 629
Lsp1109I GCAGC 3 cut(s) 69, 300, 391
LweI GCATC 2 cut(s) 109, 334
MaeI CTAG 2 cut(s) 147, 411
MaeIII GTNAC 1 cut(s) 55
MalI GATC 1 cut(s) 238
MboI GATC 1 cut(s) 236
MboII GAAGA 6 cut(s) 84, 100, 133, 443, 494, 667
MluCI AATT 4 cut(s) 101, 126, 255, 595
MnlI CCTC 8 cut(s) 21, 143, 198, 288, 395, 522, 613, 620
MseI TTAA 2 cut(s) 258, 335
MspA1I CMGCKG 1 cut(s) 382
MspI CCGG 1 cut(s) 151
MspR9I CCNGG 2 cut(s) 152, 578
Mva1269I GAATGC 1 cut(s) 574
MvaI CCWGG 1 cut(s) 578
MwoI GCNNNNNNNGC 2 cut(s) 128, 578
NciI CCSGG 1 cut(s) 152
NdeI CATATG 1 cut(s) 97
NdeII GATC 1 cut(s) 236
NlaIII CATG 3 cut(s) 222, 409, 550
NlaIV GGNNCC 1 cut(s) 155
NmuCI GTSAC 1 cut(s) 55
NspI RCATGY 1 cut(s) 222
NspV TTCGAA 1 cut(s) 505
PctI GAATGC 1 cut(s) 574
PfeI GAWTC 5 cut(s) 46, 281, 304, 414, 658
PkrI GCNGC 3 cut(s) 84, 315, 381
Psp6I CCWGG 1 cut(s) 576
PspGI CCWGG 1 cut(s) 576
PspN4I GGNNCC 1 cut(s) 155
PspPI GGNCC 2 cut(s) 154, 420
PstI CTGCAG 1 cut(s) 318
PvuII CAGCTG 1 cut(s) 382
RsaI GTAC 3 cut(s) 170, 209, 458
RsaNI GTAC 3 cut(s) 169, 208, 457
SaqAI TTAA 2 cut(s) 258, 335
SatI GCNGC 3 cut(s) 83, 314, 380
Sau3AI GATC 1 cut(s) 236
Sau96I GGNCC 2 cut(s) 154, 420
ScaI AGTACT 2 cut(s) 170, 458
ScrFI CCNGG 2 cut(s) 152, 578
SetI ASST 9 cut(s) 62, 209, 384, 396, 422, 483, 589, 634, 638
SfaNI GCATC 2 cut(s) 109, 334
SfcI CTRYAG 1 cut(s) 314
SfuI TTCGAA 1 cut(s) 505
SinI GGWCC 1 cut(s) 420
SmlI CTYRAG 1 cut(s) 349
SmoI CTYRAG 1 cut(s) 349
Sse9I AATT 4 cut(s) 101, 126, 255, 595
SspMI CTAG 2 cut(s) 147, 411
StyD4I CCNGG 2 cut(s) 150, 576
TaaI ACNGT 2 cut(s) 185, 364
TaqI TCGA 1 cut(s) 505
TasI AATT 4 cut(s) 101, 126, 255, 595
TatI WGTACW 2 cut(s) 168, 456
TfiI GAWTC 5 cut(s) 46, 281, 304, 414, 658
Tru1I TTAA 2 cut(s) 258, 335
Tru9I TTAA 2 cut(s) 258, 335
TseFI GTSAC 1 cut(s) 55
TseI GCWGC 3 cut(s) 82, 313, 379
Tsp45I GTSAC 1 cut(s) 55
TspDTI ATGAA 2 cut(s) 114, 581
VpaK11BI GGWCC 1 cut(s) 420
XceI RCATGY 1 cut(s) 222
XspI CTAG 2 cut(s) 147, 411
ZrmI AGTACT 2 cut(s) 170, 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.