pycom16g00550

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0007978
Physical Location & Seq
Forward (+)
336660 .. 338389
1730 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 633 bp
ATGTTAAAAGTGTTTGTTAATGGTGAGGGAGTTTCAGTGCAGAGGTTGAAGAAAGCTGGGAGGTTGGTTGATAAGTACTTGGGTGAAATATCACCTGATCAGAATCTCAAGATTTCTAAGTTTCTGGGAGTTGCAGAGAGCTTGCCAGATTCTGCCAGGGACTGCTTTGATTGGGTCTACAGAGCCATTGATATCTATCTTGAGGCTCATCCAAATCTTTCGTTCGAAGAGCGGTCGAGATTATGCAGATGCTTGAATTATGAGAAGCTGAGCTTAGAAACATGTAAAGAGCTTGCCAAGAATCCTAAAATCCCACCAAGGGTTGCTATTCAAGCGCTTATTTCTCAAAACTCCAAAATAACACCACCACCACCACCTACACCGCGAAAACTAGTTGTAAATCAGAGTCAGCATTTCTGTTCCGATATTGTTTTGTACAAGGACGGGGGTGCGGGAGAAGAGAGCTCGGCGGAAGAGGATTCGATGTCGTTAGACTTGCAGAGGATGCAATGGAGGGTGGTGGAGTTGGAGAAACTGTGCAGGCAAATGAAGGGGCAAATGTCAAGGATGGTAAAACACAATGTCTTGACTGCCACTCCAACTCACAGCAGAGCTCTGCCTAGACTTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB NPH3

Protein Analysis

211

Amino Acids

23.8

Weight (kDa)

8.95

Isoelectric Point (pI)

34.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 12 - 95 5e-34 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 232
AccI GTMKAC 1 cut(s) 177
AccII CGCG 1 cut(s) 385
AciI CCGC 4 cut(s) 232, 383, 452, 470
AfaI GTAC 2 cut(s) 77, 437
AfeI AGCGCT 1 cut(s) 336
AfiI CCNNNNNNNGG 1 cut(s) 319
AflIII ACRYGT 1 cut(s) 281
AgsI TTSAA 3 cut(s) 49, 256, 332
AhlI ACTAGT 1 cut(s) 391
AjnI CCWGG 1 cut(s) 155
AluBI AGCT 7 cut(s) 56, 141, 268, 273, 292, 465, 614
AluI AGCT 7 cut(s) 56, 141, 268, 273, 292, 465, 614
Alw21I GWGCWC 2 cut(s) 467, 616
AlwNI CAGNNNCTG 2 cut(s) 152, 162
Aor51HI AGCGCT 1 cut(s) 336
AspLEI GCGC 1 cut(s) 337
AsuHPI GGTGA 3 cut(s) 35, 84, 95
AsuII TTCGAA 1 cut(s) 225
BanII GRGCYC 2 cut(s) 467, 616
Bbv12I GWGCWC 2 cut(s) 467, 616
BccI CCATC 1 cut(s) 562
BciT130I CCWGG 1 cut(s) 157
BclI TGATCA 1 cut(s) 97
BcuI ACTAGT 1 cut(s) 391
BfaI CTAG 2 cut(s) 392, 621
BfmI CTRYAG 1 cut(s) 178
BfoI RGCGCY 1 cut(s) 338
BlpI GCTNAGC 1 cut(s) 269
BmcAI AGTACT 1 cut(s) 77
Bme1390I CCNGG 1 cut(s) 157
BmrFI CCNGG 1 cut(s) 157
BmsI GCATC 2 cut(s) 239, 495
Bpu1102I GCTNAGC 1 cut(s) 269
Bpu14I TTCGAA 1 cut(s) 225
BpuEI CTTGAG 2 cut(s) 92, 221
BsaBI GATNNNNATC 2 cut(s) 102, 195
BsaJI CCNNGG 2 cut(s) 156, 317
BsaXI ACNNNNNCTCC 6 cut(s) 21, 51, 521, 551, 580, 610
Bsc4I CCNNNNNNNGG 1 cut(s) 319
Bse3DI GCAATG 1 cut(s) 515
Bse8I GATNNNNATC 2 cut(s) 102, 195
BseBI CCWGG 1 cut(s) 157
BseDI CCNNGG 2 cut(s) 156, 317
BseGI GGATG 3 cut(s) 208, 510, 573
BseJI GATNNNNATC 2 cut(s) 102, 195
BseLI CCNNNNNNNGG 1 cut(s) 319
BseMI GCAATG 1 cut(s) 515
BseMII CTCAG 1 cut(s) 260
BseYI CCCAGC 1 cut(s) 56
BsgI GTGCAG 2 cut(s) 59, 559
Bsh1236I CGCG 1 cut(s) 385
Bsh1285I CGRYCG 1 cut(s) 236
BsiEI CGRYCG 1 cut(s) 236
BsiHKAI GWGCWC 2 cut(s) 467, 616
BslFI GGGAC 1 cut(s) 173
BslI CCNNNNNNNGG 1 cut(s) 319
BsmFI GGGAC 1 cut(s) 173
Bsp119I TTCGAA 1 cut(s) 225
Bsp1286I GDGCHC 2 cut(s) 467, 616
Bsp1407I TGTACA 1 cut(s) 435
Bsp143I GATC 1 cut(s) 97
Bsp1720I GCTNAGC 1 cut(s) 269
BspACI CCGC 4 cut(s) 232, 383, 452, 470
BspCNI CTCAG 1 cut(s) 261
BspFNI CGCG 1 cut(s) 385
BspQI GCTCTTC 1 cut(s) 222
BspT104I TTCGAA 1 cut(s) 225
BsrBI CCGCTC 1 cut(s) 232
BsrDI GCAATG 1 cut(s) 515
BsrGI TGTACA 1 cut(s) 435
BssECI CCNNGG 2 cut(s) 156, 317
BssMI GATC 1 cut(s) 97
BssT1I CCWWGG 1 cut(s) 317
Bst2UI CCWGG 1 cut(s) 157
Bst4CI ACNGT 1 cut(s) 537
Bst6I CTCTTC 3 cut(s) 222, 453, 468
BstAPI GCANNNNNTGC 1 cut(s) 505
BstAUI TGTACA 1 cut(s) 435
BstBI TTCGAA 1 cut(s) 225
BstC8I GCNNGC 3 cut(s) 143, 294, 542
BstDEI CTNAG 3 cut(s) 117, 269, 274
BstF5I GGATG 3 cut(s) 208, 510, 573
BstFNI CGCG 1 cut(s) 385
BstH2I RGCGCY 1 cut(s) 338
BstHHI GCGC 1 cut(s) 337
BstKTI GATC 1 cut(s) 100
BstMBI GATC 1 cut(s) 97
BstMCI CGRYCG 1 cut(s) 236
BstMWI GCNNNNNNNGC 2 cut(s) 332, 505
BstNI CCWGG 1 cut(s) 157
BstNSI RCATGY 1 cut(s) 285
BstSCI CCNGG 1 cut(s) 155
BstSFI CTRYAG 1 cut(s) 178
BstUI CGCG 1 cut(s) 385
BtsCI GGATG 3 cut(s) 208, 510, 573
BtsIMutI CAGTG 1 cut(s) 42
Cac8I GCNNGC 3 cut(s) 143, 294, 542
CaiI CAGNNNCTG 2 cut(s) 152, 162
CfoI GCGC 1 cut(s) 337
Csp6I GTAC 2 cut(s) 76, 436
CviAII CATG 1 cut(s) 282
CviJI RGCY 9 cut(s) 56, 141, 185, 206, 268, 273, 292, 465, 614
CviKI_1 RGCY 9 cut(s) 56, 141, 185, 206, 268, 273, 292, 465, 614
CviQI GTAC 2 cut(s) 76, 436
DdeI CTNAG 3 cut(s) 117, 269, 274
DpnI GATC 1 cut(s) 99
DpnII GATC 1 cut(s) 97
Eam1104I CTCTTC 3 cut(s) 222, 453, 468
EarI CTCTTC 3 cut(s) 222, 453, 468
EciI GGCGGA 1 cut(s) 485
Ecl136II GAGCTC 2 cut(s) 465, 614
Eco130I CCWWGG 1 cut(s) 317
Eco24I GRGCYC 2 cut(s) 467, 616
Eco32I GATATC 1 cut(s) 193
Eco47III AGCGCT 1 cut(s) 336
Eco53kI GAGCTC 2 cut(s) 465, 614
EcoICRI GAGCTC 2 cut(s) 465, 614
EcoRII CCWGG 1 cut(s) 155
EcoRV GATATC 1 cut(s) 193
EcoT14I CCWWGG 1 cut(s) 317
EcoT38I GRGCYC 2 cut(s) 467, 616
ErhI CCWWGG 1 cut(s) 317
FaeI CATG 1 cut(s) 285
FaiI YATR 3 cut(s) 244, 261, 283
FalI AAGNNNNNCTT 2 cut(s) 257, 289
FaqI GGGAC 1 cut(s) 173
FatI CATG 1 cut(s) 281
FauI CCCGC 1 cut(s) 445
FbaI TGATCA 1 cut(s) 97
FblI GTMKAC 1 cut(s) 177
FokI GGATG 3 cut(s) 195, 517, 580
FriOI GRGCYC 2 cut(s) 467, 616
FspBI CTAG 2 cut(s) 392, 621
GlaI GCGC 1 cut(s) 336
GsaI CCCAGC 1 cut(s) 60
HaeII RGCGCY 1 cut(s) 338
HhaI GCGC 1 cut(s) 337
Hin1II CATG 1 cut(s) 285
Hin6I GCGC 1 cut(s) 335
HinP1I GCGC 1 cut(s) 335
HinfI GANTC 5 cut(s) 103, 149, 301, 406, 479
HphI GGTGA 3 cut(s) 35, 84, 95
Hpy166II GTNNAC 1 cut(s) 178
Hpy188I TCNGA 3 cut(s) 102, 405, 424
Hpy188III TCNNGA 4 cut(s) 109, 200, 237, 586
Hpy8I GTNNAC 1 cut(s) 178
HpyAV CCTTC 1 cut(s) 544
HpyCH4III ACNGT 1 cut(s) 537
HpyCH4V TGCA 6 cut(s) 40, 134, 246, 499, 508, 540
HpyF10VI GCNNNNNNNGC 2 cut(s) 332, 505
HpyF3I CTNAG 3 cut(s) 117, 269, 274
Hsp92II CATG 1 cut(s) 285
HspAI GCGC 1 cut(s) 335
Ksp22I TGATCA 1 cut(s) 97
Kzo9I GATC 1 cut(s) 97
LguI GCTCTTC 1 cut(s) 222
LpnPI CCDG 7 cut(s) 42, 108, 110, 142, 159, 169, 526
LweI GCATC 2 cut(s) 239, 495
MaeI CTAG 2 cut(s) 392, 621
MalI GATC 1 cut(s) 99
MbiI CCGCTC 1 cut(s) 232
MboI GATC 1 cut(s) 97
MboII GAAGA 4 cut(s) 61, 239, 470, 485
MhlI GDGCHC 2 cut(s) 467, 616
MluCI AATT 1 cut(s) 256
MlyI GAGTC 1 cut(s) 415
MmeI TCCRAC 2 cut(s) 507, 623
MnlI CCTC 7 cut(s) 19, 36, 54, 196, 469, 495, 507
MseI TTAA 2 cut(s) 5, 18
MspR9I CCNGG 1 cut(s) 157
MvaI CCWGG 1 cut(s) 157
MvnI CGCG 1 cut(s) 385
MwoI GCNNNNNNNGC 2 cut(s) 332, 505
NdeII GATC 1 cut(s) 97
NlaIII CATG 1 cut(s) 285
NmeAIII GCCGAG 1 cut(s) 446
NspI RCATGY 1 cut(s) 285
NspV TTCGAA 1 cut(s) 225
PciI ACATGT 1 cut(s) 281
PciSI GCTCTTC 1 cut(s) 222
PfeI GAWTC 4 cut(s) 103, 149, 301, 479
PleI GAGTC 1 cut(s) 414
PpsI GAGTC 1 cut(s) 414
PscI ACATGT 1 cut(s) 281
Psp124BI GAGCTC 2 cut(s) 467, 616
Psp6I CCWGG 1 cut(s) 155
PspFI CCCAGC 1 cut(s) 56
PspGI CCWGG 1 cut(s) 155
PstNI CAGNNNCTG 2 cut(s) 152, 162
RsaI GTAC 2 cut(s) 77, 437
RsaNI GTAC 2 cut(s) 76, 436
SacI GAGCTC 2 cut(s) 467, 616
SapI GCTCTTC 1 cut(s) 222
SaqAI TTAA 2 cut(s) 5, 18
Sau3AI GATC 1 cut(s) 97
ScaI AGTACT 1 cut(s) 77
SchI GAGTC 1 cut(s) 415
ScrFI CCNGG 1 cut(s) 157
SduI GDGCHC 2 cut(s) 467, 616
SfaNI GCATC 2 cut(s) 239, 495
SfcI CTRYAG 1 cut(s) 178
SfuI TTCGAA 1 cut(s) 225
SmlI CTYRAG 2 cut(s) 107, 200
SmoI CTYRAG 2 cut(s) 107, 200
SpeI ACTAGT 1 cut(s) 391
Sse9I AATT 1 cut(s) 256
SsiI CCGC 4 cut(s) 232, 383, 452, 470
SspMI CTAG 2 cut(s) 392, 621
SstI GAGCTC 2 cut(s) 467, 616
StyD4I CCNGG 1 cut(s) 155
StyI CCWWGG 1 cut(s) 317
TaaI ACNGT 1 cut(s) 537
TaqI TCGA 3 cut(s) 225, 236, 482
TasI AATT 1 cut(s) 256
TatI WGTACW 2 cut(s) 75, 435
TfiI GAWTC 4 cut(s) 103, 149, 301, 479
Tru1I TTAA 2 cut(s) 5, 18
Tru9I TTAA 2 cut(s) 5, 18
TscAI CASTG 1 cut(s) 42
TspDTI ATGAA 1 cut(s) 563
TspRI CASTG 1 cut(s) 42
XceI RCATGY 1 cut(s) 285
XmiI GTMKAC 1 cut(s) 177
XspI CTAG 2 cut(s) 392, 621
ZrmI AGTACT 1 cut(s) 77
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.