Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0007353

Physical Location & Seq

Genomic Coordinates Forward (+)

1028538 .. 1032629

Gene Structure 4092 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2073 bp

ATGGTTACACTCACCACGAAACGAATCAAACGCCGGCCCAGAACTGAAAAGCGCGCCAAGAAGCCGCGCATATCAATTCTCATGGCCATCGACTCTCTCGTCCTCGGCGCCGGGCAAGAGGTCGGTAAGAGCTGCGTGGTGGTGACAATCAATGGGAAGCGAATCATGTTCGACTGCGGAATGCACATGGGCTATCTCGACCACCGCCGCTACCCCGATTTCTCTCGCATTCCCAAATCGCAGAACGAGTCGAACTTCGATCACTCCCTTACTTGCATCATCATCACCCACTTTCACTTAGATCACGTTGGGGCTCTTCCTTACTTCACCGAAGTCTGCGGTTATCAGGGTCCAATTTACATGACGTATCCGACAAAGGCATTGTCTCCTATAATGTTGGAGGACTATCGAAAAGTGATGGTGGAGCGAAGGGGCGAGGAAGAGCAGTTTTCGTCTTATCACATTGCCGAGTGCATGAAGAAAGTAGTACCAGTGGATCTGAAGCAGACAGTGCAGGTTGATAAAGACCTTCAAATCCGTGCATACTATGCAGGACATGTTCTTGGAGCCGCAATGTTTTACGCAAAGGTGGGAGACGCCACTATGGTGTACACGGGAGACTATAATATGACACCAGATAGGCATCTTGGAGCAGCTCAAATTGACCGACTAAATTTGGACCTTCTTATTTCAGAATCCACGTATGCAACAACCATACGCGATTCAAAATATGCCCGGGAAAGAGAATTTCTCAGAGCTGTTCATAAATGTGTTGCTGCCGGTGGAAAAGTTCTAATTCCAACTTTTGCTCTTGGAAGAGCTCAGGAACTGTGTATCTTGTTGGAAGATTACTGGGAGCGCATGAATCTAAAGGTTCCTATTTACTTCTCAGCAGGTTTGACCCTTCAAGCCAATATGTATTATAGGATGCTTATCAGTTGGACCAGTCAGAAAGTCAAAGAAACGTACTCCACGCATAATGCCTTTGATTTTAAGAACGCTCACAAGTTTGATCGTTCGATGATGCATGCTCCTGGACCCTGTGTTCTGTTTGCCACACCAGGGATGATCAGTGGTGGCTTTTCACTTGAGGTTTTCAAGCACTGGGCTCCTTCGGCGATGAATCTTGTTACACTTCCCGGGTATTGTGTGGCTGGAACCATAGGGCATAAATTGATGTCGGGAAAACCCACCAAAATTGATCTGGACAAGGACACACAAATTGATGTTCGATGCCAGATTCATCAGTTGTCTTTCAGTCCTCACACAGACGCCAAAGGAATTATGGATCTTGTAAAATTTCTCTCCCCGAAGCACGTCATACTTGTTCATGGCGAGAAGCCGAGGATGGCGATTCTAAAAGGAAAAATCCAGTCGGAGCTGGGAATTCAGTGTTACGACCCTGCAAACAATGAGACCGTGTCAGTTCCATCAACCCACTACGCGAAAGCAGTAGCTTCCAACGTGTTCATCCGAAGCTGTTCGAATCCAAACTTCAAGTTCTCGAAAAGAAGTTCAGAAGATGAACACGGTTTGAATTTGGGAACCAGAAATTCGCCTCCAGGACTGCAAGTAAGCGATGAAAGGGCAGCAGAAGGGGTTTTGGTTATGGAGAAAAGCAAAAAAACGAAGGTAGTACACCAAGACGAGCTTATGCTTATGTTAGGAGAGAAAAAGCATCAAGTACAATTTGCTTATTGCTGCCCTGTTCAAACTGGCAACTTGAAAGAGGCCAAAAGCACTTCGGGAAACGACGATCAGCTTTGCAAATCTGAGAGAGGCTCTCGGCTTCTCCGCAGATTATCTGCGGAACTTTCAAATGAGCTTTCTGAGGGGAACATCCAAGACTTTGAGGATCACCTTGAAGTGGCATCGTTTCGCGCATCCGTTTGCTTGAAGGAAAACTGCCCTCACAGATTACTGGATGGCCATGGCGTTCGGAATAAATCTGAAGAAGCAGTTTATTTCTGCTGCAGTTGGGCAATGGCAGATGAGAAGCTTGCATGGCAAGTCATTTCAATCTGTCAAAATTTCTCCATGCACGAGCAAAACAAGGGGGCTTCTACCCTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000741 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006996 GO:0006997 GO:0007275 GO:0008150 GO:0009553 GO:0009559 GO:0009561 GO:0009987 GO:0010197 GO:0016043 GO:0030154 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048229 GO:0048284 GO:0048856 GO:0048869 GO:0071840

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Beta-Casp Lactamase_B_6 RMMBL zf-RING_2

Protein Analysis

691

Amino Acids

77.7

Weight (kDa)

8.74

Isoelectric Point (pI)

39.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lactamase_B	PF00753	42 - 216	1.1e-11	Metallo-beta-lactamase superfamily
Anti-Pycsar_Apyc1	PF23023	44 - 214	3.7e-08	Anti-Pycsar protein Apyc1
Lactamase_B_6	PF16661	49 - 221	2.6e-18	Metallo-beta-lactamase superfamily domain
Beta-Casp	PF10996	274 - 392	1e-26	Beta-Casp domain
RMMBL	PF07521	408 - 466	2.4e-15	Zn-dependent metallo-hydrolase RNA specificity domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0007353)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G01730 AT2G01730
fragaria_vesca	FvH4_4g33520
malus_domestica	MD16G1018300.v1.1
prunus_persica	Prupe.1G333600_v2.0.a1
pyrus_communis	pycom16g01670
rosa_chinensis	RchiOBHm_Chr3g0480131 RchiOBHm_Chr4g0443031
rosa_laevigata	RLG00000005961
rosa_multiflora	Rmu_co8263599.1_g000001 Rmu_sc0006604.1_g000001 Rmu_sc0006604.1_g000004 Rmu_sc0008055.1_g000002 Rmu_sc0011261.1_g000006
rosa_roxburghii	Rroxscaffold_5G00383660
rosa_rugosa	Rorug04G0343900
rosa_samantha	Rh3AG232100 Rh3CG261900 Rh3DG257700 Rh4AG398100 Rh4BG409600 Rh4CG425100 Rh4DG404400
rosa_wichuraiana	Rw3G020840 Rw4G034290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	98
Acc36I	ACCTGC	2 cut(s)	505, 884
AccB1I	GGYRCC	1 cut(s)	107
AccII	CGCG	5 cut(s)	54, 67, 720, 1445, 1881
AciI	CCGC	8 cut(s)	65, 177, 205, 208, 339, 570, 1795, 1808
AclWI	GGATC	3 cut(s)	504, 1296, 1863
AcoI	YGGCCR	2 cut(s)	84, 1927
AcsI	RAATTY	7 cut(s)	673, 746, 1298, 1386, 1537, 1552, 2029
AcuI	CTGAAG	2 cut(s)	521, 1971
AcyI	GRCGYC	3 cut(s)	108, 597, 1272
AfaI	GTAC	5 cut(s)	489, 611, 968, 1638, 1686
AfiI	CCNNNNNNNGG	2 cut(s)	1062, 1867
AflIII	ACRYGT	2 cut(s)	556, 1464
AjiI	CACGTC	1 cut(s)	1318
AjnI	CCWGG	3 cut(s)	1033, 1060, 1561
AleI	CACNNNNGTG	1 cut(s)	605
AloI	GAACNNNNNNTCC	2 cut(s)	1029, 1061
Alw21I	GWGCWC	1 cut(s)	823
Alw26I	GTCTC	4 cut(s)	390, 588, 612, 1409
AlwI	GGATC	3 cut(s)	504, 1296, 1863
AlwNI	CAGNNNCTG	1 cut(s)	829
Ama87I	CYCGRG	2 cut(s)	735, 1139
AoxI	GGCC	4 cut(s)	35, 84, 1731, 1927
ApeKI	GCWGC	6 cut(s)	132, 653, 776, 1589, 1701, 1971
ApoI	RAATTY	7 cut(s)	673, 746, 1298, 1386, 1537, 1552, 2029
ArsI	GACNNNNNNTTYG	2 cut(s)	368, 400
Asp700I	GAANNNNTTC	1 cut(s)	1480
AspLEI	GCGC	6 cut(s)	54, 56, 69, 110, 861, 1883
AspS9I	GGNCC	5 cut(s)	36, 350, 679, 942, 1037
AsuC2I	CCSGG	5 cut(s)	112, 736, 737, 1140, 1141
AsuHPI	GGTGA	5 cut(s)	4, 154, 277, 319, 1850
AsuII	TTCGAA	1 cut(s)	1484
AvaI	CYCGRG	2 cut(s)	735, 1139
AvaII	GGWCC	4 cut(s)	350, 679, 942, 1037
BalI	TGGCCA	2 cut(s)	86, 1929
BanI	GGYRCC	1 cut(s)	107
BanII	GRGCYC	3 cut(s)	316, 823, 1111
BauI	CACGAG	1 cut(s)	2042
Bbv12I	GWGCWC	1 cut(s)	823
BbvI	GCAGC	6 cut(s)	119, 665, 763, 1601, 1688, 1958
BccI	CCATC	5 cut(s)	95, 412, 1342, 1438, 1919
BciT130I	CCWGG	3 cut(s)	1035, 1062, 1563
BciVI	GTATCC	1 cut(s)	378
BclI	TGATCA	1 cut(s)	1068
BcnI	CCSGG	5 cut(s)	112, 736, 737, 1140, 1141
BcoDI	GTCTC	4 cut(s)	390, 588, 612, 1409
BfmI	CTRYAG	2 cut(s)	1972, 2069
BfoI	RGCGCY	1 cut(s)	111
BfuAI	ACCTGC	2 cut(s)	505, 884
BfuI	GTATCC	1 cut(s)	378
BisI	GCNGC	9 cut(s)	65, 133, 208, 570, 654, 777, 1590, 1702, 1972
BlsI	GCNGC	9 cut(s)	66, 134, 209, 571, 655, 778, 1591, 1703, 1973
Bme1390I	CCNGG	8 cut(s)	112, 736, 737, 1035, 1062, 1140, 1141, 1563
Bme18I	GGWCC	4 cut(s)	350, 679, 942, 1037
BmeT110I	CYCGRG	2 cut(s)	735, 1139
BmgBI	CACGTC	1 cut(s)	1318
BmgT120I	GGNCC	5 cut(s)	36, 350, 679, 942, 1037
BmiI	GGNNCC	8 cut(s)	109, 351, 568, 876, 1039, 1110, 1159, 1546
BmrFI	CCNGG	8 cut(s)	112, 736, 737, 1035, 1062, 1140, 1141, 1563
BmrI	ACTGGG	2 cut(s)	862, 1114
BmsI	GCATC	8 cut(s)	285, 652, 918, 1014, 1223, 1687, 1880, 1892
BmuI	ACTGGG	2 cut(s)	862, 1114
BplI	GAGNNNNNCTC	6 cut(s)	735, 767, 1766, 1798, 1768, 1800
BpmI	CTGGAG	1 cut(s)	1545
Bpu10I	CCTNAGC	1 cut(s)	822
Bpu14I	TTCGAA	1 cut(s)	1484
BpuEI	CTTGAG	1 cut(s)	1109
BpuMI	CCSGG	5 cut(s)	112, 736, 737, 1140, 1141
BsaAI	YACGTR	1 cut(s)	702
BsaBI	GATNNNNATC	2 cut(s)	642, 932
BsaHI	GRCGYC	3 cut(s)	108, 597, 1272
BsaI	GGTCTC	1 cut(s)	1409
BsaJI	CCNNGG	6 cut(s)	103, 735, 1061, 1139, 1343, 1930
Bsc4I	CCNNNNNNNGG	2 cut(s)	1062, 1867
Bse118I	RCCGGY	2 cut(s)	33, 779
Bse1I	ACTGG	7 cut(s)	491, 857, 945, 1109, 1372, 1720, 1926
Bse3DI	GCAATG	3 cut(s)	462, 579, 1989
Bse8I	GATNNNNATC	2 cut(s)	642, 932
BseBI	CCWGG	3 cut(s)	1035, 1062, 1563
BseDI	CCNNGG	6 cut(s)	103, 735, 1061, 1139, 1343, 1930
BseGI	GGATG	7 cut(s)	933, 1071, 1353, 1470, 1839, 1883, 1930
BseJI	GATNNNNATC	2 cut(s)	642, 932
BseLI	CCNNNNNNNGG	2 cut(s)	1062, 1867
BseMI	GCAATG	3 cut(s)	462, 579, 1989
BseMII	CTCAG	5 cut(s)	766, 836, 903, 1764, 1821
BseNI	ACTGG	7 cut(s)	491, 857, 945, 1109, 1372, 1720, 1926
BsePI	GCGCGC	1 cut(s)	52
BseXI	GCAGC	6 cut(s)	119, 665, 763, 1601, 1688, 1958
BseYI	CCCAGC	1 cut(s)	1381
BsgI	GTGCAG	1 cut(s)	533
Bsh1236I	CGCG	5 cut(s)	54, 67, 720, 1445, 1881
BshFI	GGCC	4 cut(s)	37, 86, 1733, 1929
BshNI	GGYRCC	1 cut(s)	107
BsiHKAI	GWGCWC	1 cut(s)	823
BsiHKCI	CYCGRG	2 cut(s)	735, 1139
BsiSI	CCGG	5 cut(s)	34, 111, 736, 780, 1140
BslI	CCNNNNNNNGG	2 cut(s)	1062, 1867
BsmAI	GTCTC	4 cut(s)	390, 588, 612, 1409
BsmBI	CGTCTC	1 cut(s)	588
BsmI	GAATGC	2 cut(s)	186, 228
BsnI	GGCC	4 cut(s)	37, 86, 1733, 1929
Bso31I	GGTCTC	1 cut(s)	1409
BsoBI	CYCGRG	2 cut(s)	735, 1139
Bsp119I	TTCGAA	1 cut(s)	1484
Bsp1286I	GDGCHC	3 cut(s)	316, 823, 1111
Bsp1407I	TGTACA	1 cut(s)	609
Bsp143I	GATC	9 cut(s)	259, 301, 496, 1012, 1068, 1201, 1288, 1756, 1855
Bsp19I	CCATGG	1 cut(s)	1930
BspACI	CCGC	8 cut(s)	65, 177, 205, 208, 339, 570, 1795, 1808
BspANI	GGCC	4 cut(s)	37, 86, 1733, 1929
BspCNI	CTCAG	5 cut(s)	765, 835, 902, 1765, 1822
BspFNI	CGCG	5 cut(s)	54, 67, 720, 1445, 1881
BspLI	GGNNCC	8 cut(s)	109, 351, 568, 876, 1039, 1110, 1159, 1546
BspMAI	CTGCAG	1 cut(s)	1976
BspMI	ACCTGC	2 cut(s)	505, 884
BspPI	GGATC	3 cut(s)	504, 1296, 1863
BspQI	GCTCTTC	3 cut(s)	321, 435, 811
BspT104I	TTCGAA	1 cut(s)	1484
BspT107I	GGYRCC	1 cut(s)	107
BspTNI	GGTCTC	1 cut(s)	1409
BsrDI	GCAATG	3 cut(s)	462, 579, 1989
BsrFI	RCCGGY	2 cut(s)	33, 779
BsrGI	TGTACA	1 cut(s)	609
BsrI	ACTGG	7 cut(s)	491, 857, 945, 1109, 1372, 1720, 1926
BssAI	RCCGGY	2 cut(s)	33, 779
BssECI	CCNNGG	6 cut(s)	103, 735, 1061, 1139, 1343, 1930
BssHII	GCGCGC	1 cut(s)	52
BssMI	GATC	9 cut(s)	259, 301, 496, 1012, 1068, 1201, 1288, 1756, 1855
BssNI	GRCGYC	3 cut(s)	108, 597, 1272
BssSI	CACGAG	1 cut(s)	2042
BssT1I	CCWWGG	1 cut(s)	1930
Bst2BI	CACGAG	1 cut(s)	2042
Bst2UI	CCWGG	3 cut(s)	1035, 1062, 1563
Bst4CI	ACNGT	4 cut(s)	511, 831, 1420, 1532
Bst6I	CTCTTC	3 cut(s)	321, 435, 811
BstACI	GRCGYC	3 cut(s)	108, 597, 1272
BstAPI	GCANNNNNTGC	2 cut(s)	511, 548
BstAUI	TGTACA	1 cut(s)	609
BstBAI	YACGTR	1 cut(s)	702
BstBI	TTCGAA	1 cut(s)	1484
BstC8I	GCNNGC	4 cut(s)	35, 54, 1029, 2001
BstDEI	CTNAG	6 cut(s)	298, 752, 822, 889, 1773, 1830
BstDSI	CCRYGG	1 cut(s)	1930
BstF5I	GGATG	7 cut(s)	933, 1071, 1353, 1470, 1839, 1883, 1930
BstFNI	CGCG	5 cut(s)	54, 67, 720, 1445, 1881
BstH2I	RGCGCY	1 cut(s)	111
BstHHI	GCGC	6 cut(s)	54, 56, 69, 110, 861, 1883
BstKTI	GATC	9 cut(s)	262, 304, 499, 1015, 1071, 1204, 1291, 1759, 1858
BstMAI	GTCTC	4 cut(s)	390, 588, 612, 1409
BstMBI	GATC	9 cut(s)	259, 301, 496, 1012, 1068, 1201, 1288, 1756, 1855
BstMWI	GCNNNNNNNGC	4 cut(s)	511, 548, 1115, 2005
BstNI	CCWGG	3 cut(s)	1035, 1062, 1563
BstNSI	RCATGY	2 cut(s)	560, 1031
BstSCI	CCNGG	8 cut(s)	110, 734, 735, 1033, 1060, 1138, 1139, 1561
BstSFI	CTRYAG	2 cut(s)	1972, 2069
BstUI	CGCG	5 cut(s)	54, 67, 720, 1445, 1881
BstV1I	GCAGC	6 cut(s)	119, 665, 763, 1601, 1688, 1958
BstX2I	RGATCY	2 cut(s)	496, 1288
BstYI	RGATCY	2 cut(s)	496, 1288
BsuI	GTATCC	1 cut(s)	378
BsuRI	GGCC	4 cut(s)	37, 86, 1733, 1929
BtgI	CCRYGG	1 cut(s)	1930
BtgZI	GCGATG	2 cut(s)	1133, 1593
BtrI	CACGTC	1 cut(s)	1318
BtsCI	GGATG	7 cut(s)	933, 1071, 1353, 1470, 1839, 1883, 1930
BtsIMutI	CAGTG	5 cut(s)	498, 516, 1078, 1102, 1397
BveI	ACCTGC	2 cut(s)	505, 884
Cac8I	GCNNGC	4 cut(s)	35, 54, 1029, 2001
CaiI	CAGNNNCTG	1 cut(s)	829
CfoI	GCGC	6 cut(s)	54, 56, 69, 110, 861, 1883
Cfr10I	RCCGGY	2 cut(s)	33, 779
Cfr13I	GGNCC	5 cut(s)	36, 350, 679, 942, 1037
Cfr9I	CCCGGG	2 cut(s)	735, 1139
CseI	GACGC	2 cut(s)	605, 1280
Csp6I	GTAC	5 cut(s)	488, 610, 967, 1637, 1685
CspCI	CAANNNNNGTGG	2 cut(s)	1180, 1215
CviQI	GTAC	5 cut(s)	488, 610, 967, 1637, 1685
DdeI	CTNAG	6 cut(s)	298, 752, 822, 889, 1773, 1830
DinI	GGCGCC	1 cut(s)	109
DpnI	GATC	9 cut(s)	261, 303, 498, 1014, 1070, 1203, 1290, 1758, 1857
DpnII	GATC	9 cut(s)	259, 301, 496, 1012, 1068, 1201, 1288, 1756, 1855
DrdI	GACNNNNNNGTC	1 cut(s)	98
DseDI	GACNNNNNNGTC	1 cut(s)	98
EaeI	YGGCCR	2 cut(s)	84, 1927
Eam1104I	CTCTTC	3 cut(s)	321, 435, 811
EarI	CTCTTC	3 cut(s)	321, 435, 811
Ecl136II	GAGCTC	1 cut(s)	821
Eco130I	CCWWGG	1 cut(s)	1930
Eco24I	GRGCYC	3 cut(s)	316, 823, 1111
Eco31I	GGTCTC	1 cut(s)	1409
Eco47I	GGWCC	4 cut(s)	350, 679, 942, 1037
Eco53kI	GAGCTC	1 cut(s)	821
Eco57I	CTGAAG	2 cut(s)	521, 1971
Eco88I	CYCGRG	2 cut(s)	735, 1139
EcoICRI	GAGCTC	1 cut(s)	821
EcoRI	GAATTC	1 cut(s)	1386
EcoRII	CCWGG	3 cut(s)	1033, 1060, 1561
EcoT14I	CCWWGG	1 cut(s)	1930
EcoT22I	ATGCAT	1 cut(s)	1029
EcoT38I	GRGCYC	3 cut(s)	316, 823, 1111
EgeI	GGCGCC	1 cut(s)	109
EheI	GGCGCC	1 cut(s)	109
ErhI	CCWWGG	1 cut(s)	1930
Esp3I	CGTCTC	1 cut(s)	588
FalI	AAGNNNNNCTT	2 cut(s)	1635, 1667
FbaI	TGATCA	1 cut(s)	1068
Fnu4HI	GCNGC	9 cut(s)	65, 133, 208, 570, 654, 777, 1590, 1702, 1972
FokI	GGATG	7 cut(s)	940, 1078, 1360, 1457, 1826, 1870, 1937
FriOI	GRGCYC	3 cut(s)	316, 823, 1111
Fsp4HI	GCNGC	9 cut(s)	65, 133, 208, 570, 654, 777, 1590, 1702, 1972
GlaI	GCGC	6 cut(s)	53, 55, 68, 109, 860, 1882
GluI	GCNGC	9 cut(s)	65, 133, 208, 570, 654, 777, 1590, 1702, 1972
GsaI	CCCAGC	1 cut(s)	1385
GsuI	CTGGAG	1 cut(s)	1545
HaeII	RGCGCY	1 cut(s)	111
HaeIII	GGCC	4 cut(s)	37, 86, 1733, 1929
HapII	CCGG	5 cut(s)	34, 111, 736, 780, 1140
HgaI	GACGC	2 cut(s)	605, 1280
HhaI	GCGC	6 cut(s)	54, 56, 69, 110, 861, 1883
Hin1I	GRCGYC	3 cut(s)	108, 597, 1272
Hin6I	GCGC	6 cut(s)	52, 54, 67, 108, 859, 1881
HinP1I	GCGC	6 cut(s)	52, 54, 67, 108, 859, 1881
HindIII	AAGCTT	1 cut(s)	1997
HpaII	CCGG	5 cut(s)	34, 111, 736, 780, 1140
HphI	GGTGA	5 cut(s)	4, 154, 277, 319, 1850
Hpy166II	GTNNAC	3 cut(s)	610, 612, 1639
Hpy188III	TCNNGA	6 cut(s)	197, 824, 1182, 1205, 1504, 1746
Hpy8I	GTNNAC	3 cut(s)	610, 612, 1639
Hpy99I	CGWCG	1 cut(s)	1757
HpyAV	CCTTC	8 cut(s)	423, 539, 692, 914, 1122, 1589, 1624, 1891
HpyCH4III	ACNGT	4 cut(s)	511, 831, 1420, 1532
HpyCH4IV	ACGT	6 cut(s)	306, 365, 701, 965, 1317, 1464
HpyF10VI	GCNNNNNNNGC	4 cut(s)	511, 548, 1115, 2005
HpyF3I	CTNAG	6 cut(s)	298, 752, 822, 889, 1773, 1830
HpySE526I	ACGT	6 cut(s)	306, 365, 701, 965, 1317, 1464
Hsp92I	GRCGYC	3 cut(s)	108, 597, 1272
HspAI	GCGC	6 cut(s)	52, 54, 67, 108, 859, 1881
KasI	GGCGCC	1 cut(s)	107
KroI	GCCGGC	1 cut(s)	33
KroNI	GCCGGC	1 cut(s)	35
Ksp22I	TGATCA	1 cut(s)	1068
Kzo9I	GATC	9 cut(s)	259, 301, 496, 1012, 1068, 1201, 1288, 1756, 1855
LguI	GCTCTTC	3 cut(s)	321, 435, 811
LmnI	GCTCC	7 cut(s)	424, 566, 650, 856, 1036, 1114, 1378
Lsp1109I	GCAGC	6 cut(s)	119, 665, 763, 1601, 1688, 1958
LweI	GCATC	8 cut(s)	285, 652, 918, 1014, 1223, 1687, 1880, 1892
MaeII	ACGT	6 cut(s)	306, 365, 701, 965, 1317, 1464
MaeIII	GTNAC	4 cut(s)	4, 142, 1129, 1394
MalI	GATC	9 cut(s)	261, 303, 498, 1014, 1070, 1203, 1290, 1758, 1857
MboI	GATC	9 cut(s)	259, 301, 496, 1012, 1068, 1201, 1288, 1756, 1855
MboII	GAAGA	7 cut(s)	308, 452, 490, 828, 857, 1532, 1964
MflI	RGATCY	2 cut(s)	496, 1288
MhlI	GDGCHC	3 cut(s)	316, 823, 1111
MlsI	TGGCCA	2 cut(s)	86, 1929
MluNI	TGGCCA	2 cut(s)	86, 1929
Mly113I	GGCGCC	1 cut(s)	108
MlyI	GAGTC	2 cut(s)	86, 257
MmeI	TCCRAC	7 cut(s)	378, 395, 822, 824, 920, 1356, 1485
Mox20I	TGGCCA	2 cut(s)	86, 1929
Mph1103I	ATGCAT	1 cut(s)	1029
MroNI	GCCGGC	1 cut(s)	33
MroXI	GAANNNNTTC	1 cut(s)	1480
MscI	TGGCCA	2 cut(s)	86, 1929
MseI	TTAA	1 cut(s)	993
MslI	CAYNNNNRTG	2 cut(s)	605, 768
Msp20I	TGGCCA	2 cut(s)	86, 1929
MspI	CCGG	5 cut(s)	34, 111, 736, 780, 1140
MspR9I	CCNGG	8 cut(s)	112, 736, 737, 1035, 1062, 1140, 1141, 1563
Mva1269I	GAATGC	2 cut(s)	186, 228
MvaI	CCWGG	3 cut(s)	1035, 1062, 1563
MvnI	CGCG	5 cut(s)	54, 67, 720, 1445, 1881
MwoI	GCNNNNNNNGC	4 cut(s)	511, 548, 1115, 2005
NaeI	GCCGGC	1 cut(s)	35
NarI	GGCGCC	1 cut(s)	108
NciI	CCSGG	5 cut(s)	112, 736, 737, 1140, 1141
NcoI	CCATGG	1 cut(s)	1930
NdeII	GATC	9 cut(s)	259, 301, 496, 1012, 1068, 1201, 1288, 1756, 1855
NgoMIV	GCCGGC	1 cut(s)	33
NlaIV	GGNNCC	8 cut(s)	109, 351, 568, 876, 1039, 1110, 1159, 1546
NmeAIII	GCCGAG	4 cut(s)	84, 493, 1368, 1765
NmuCI	GTSAC	1 cut(s)	142
NsiI	ATGCAT	1 cut(s)	1029
NspI	RCATGY	2 cut(s)	560, 1031
NspV	TTCGAA	1 cut(s)	1484
OliI	CACNNNNGTG	1 cut(s)	605
PaeI	GCATGC	1 cut(s)	1031
PauI	GCGCGC	1 cut(s)	52
PciI	ACATGT	1 cut(s)	556
PciSI	GCTCTTC	3 cut(s)	321, 435, 811
PcsI	WCGNNNNNNNCGW	1 cut(s)	96
PctI	GAATGC	2 cut(s)	186, 228
PdiI	GCCGGC	1 cut(s)	35
PdmI	GAANNNNTTC	1 cut(s)	1480
PfeI	GAWTC	9 cut(s)	24, 162, 695, 722, 865, 1123, 1240, 1354, 1486
PflFI	GACNNNGTC	1 cut(s)	1420
PfoI	TCCNGGA	2 cut(s)	1033, 1561
PkrI	GCNGC	9 cut(s)	66, 134, 209, 571, 655, 778, 1591, 1703, 1973
PleI	GAGTC	2 cut(s)	86, 256
PluTI	GGCGCC	1 cut(s)	111
PpsI	GAGTC	2 cut(s)	86, 256
Ppu21I	YACGTR	1 cut(s)	702
PscI	ACATGT	1 cut(s)	556
Psp124BI	GAGCTC	1 cut(s)	823
Psp6I	CCWGG	3 cut(s)	1033, 1060, 1561
PspFI	CCCAGC	1 cut(s)	1381
PspGI	CCWGG	3 cut(s)	1033, 1060, 1561
PspN4I	GGNNCC	8 cut(s)	109, 351, 568, 876, 1039, 1110, 1159, 1546
PspPI	GGNCC	5 cut(s)	36, 350, 679, 942, 1037
PstI	CTGCAG	1 cut(s)	1976
PstNI	CAGNNNCTG	1 cut(s)	829
PsuI	RGATCY	2 cut(s)	496, 1288
PsyI	GACNNNGTC	1 cut(s)	1420
PteI	GCGCGC	1 cut(s)	52
RsaI	GTAC	5 cut(s)	489, 611, 968, 1638, 1686
RsaNI	GTAC	5 cut(s)	488, 610, 967, 1637, 1685
RseI	CAYNNNNRTG	2 cut(s)	605, 768
SacI	GAGCTC	1 cut(s)	823
SapI	GCTCTTC	3 cut(s)	321, 435, 811
SaqAI	TTAA	1 cut(s)	993
SatI	GCNGC	9 cut(s)	65, 133, 208, 570, 654, 777, 1590, 1702, 1972
Sau3AI	GATC	9 cut(s)	259, 301, 496, 1012, 1068, 1201, 1288, 1756, 1855
Sau96I	GGNCC	5 cut(s)	36, 350, 679, 942, 1037
SchI	GAGTC	2 cut(s)	86, 257
ScrFI	CCNGG	8 cut(s)	112, 736, 737, 1035, 1062, 1140, 1141, 1563
SduI	GDGCHC	3 cut(s)	316, 823, 1111
SfaNI	GCATC	8 cut(s)	285, 652, 918, 1014, 1223, 1687, 1880, 1892
SfcI	CTRYAG	2 cut(s)	1972, 2069
SfoI	GGCGCC	1 cut(s)	109
SfuI	TTCGAA	1 cut(s)	1484
SinI	GGWCC	4 cut(s)	350, 679, 942, 1037
SmaI	CCCGGG	2 cut(s)	737, 1141
SmiMI	CAYNNNNRTG	2 cut(s)	605, 768
SmlI	CTYRAG	1 cut(s)	1088
SmoI	CTYRAG	1 cut(s)	1088
SphI	GCATGC	1 cut(s)	1031
SsiI	CCGC	8 cut(s)	65, 177, 205, 208, 339, 570, 1795, 1808
SspDI	GGCGCC	1 cut(s)	107
SstI	GAGCTC	1 cut(s)	823
StyD4I	CCNGG	8 cut(s)	110, 734, 735, 1033, 1060, 1138, 1139, 1561
StyI	CCWWGG	1 cut(s)	1930
TaaI	ACNGT	4 cut(s)	511, 831, 1420, 1532
TaiI	ACGT	6 cut(s)	309, 368, 704, 968, 1320, 1467
TaqII	GACCGA	1 cut(s)	681
TatI	WGTACW	3 cut(s)	609, 1636, 1684
TauI	GCSGC	3 cut(s)	67, 210, 572
TfiI	GAWTC	9 cut(s)	24, 162, 695, 722, 865, 1123, 1240, 1354, 1486
Tru1I	TTAA	1 cut(s)	993
Tru9I	TTAA	1 cut(s)	993
TscAI	CASTG	5 cut(s)	498, 516, 1078, 1109, 1397
TseFI	GTSAC	1 cut(s)	142
TseI	GCWGC	6 cut(s)	132, 653, 776, 1589, 1701, 1971
Tsp45I	GTSAC	1 cut(s)	142
TspDTI	ATGAA	9 cut(s)	491, 752, 878, 1136, 1232, 1319, 1459, 1539, 1596
TspGWI	ACGGA	2 cut(s)	527, 1876
TspMI	CCCGGG	2 cut(s)	735, 1139
TspRI	CASTG	5 cut(s)	498, 516, 1078, 1109, 1397
Tth111I	GACNNNGTC	1 cut(s)	1420
VpaK11BI	GGWCC	4 cut(s)	350, 679, 942, 1037
XapI	RAATTY	7 cut(s)	673, 746, 1298, 1386, 1537, 1552, 2029
XceI	RCATGY	2 cut(s)	560, 1031
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1201, 1282
XmaI	CCCGGG	2 cut(s)	735, 1139
XmnI	GAANNNNTTC	1 cut(s)	1480
Zsp2I	ATGCAT	1 cut(s)	1029

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.