pycom16g02550

Heavy metal-associated isoprenylated plant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0003528
Physical Location & Seq
Reverse (-)
1692518 .. 1693689
1172 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 639 bp
ATGAGGACCGAGTCACACTACATTCTGATTATAAATATCTTTGTTCTCGCCCCAAACACACTGCCTTTGCAGCAACACATTCCCTTTCCCTCTCTCTCCTCTCTCTCTCCTTTCTTCCCCCCCCCCTACCCCTCTCTCTCTCTCTCACTAGCAAAAAAAAAAAAAAAACTCAGATCAGAATTATCAGAGTGGAGAAAAATGGGTGCTCTGGATGCTCTTTCAGACTATTTCACAATCTCAAGACCAAGAAAGAAACGAAAGCCAATGCAGACGGTTGAAATCAAGGTGAAAATGGACTGTGATGGCTGTGAAAGGAGAGTCAAAAATGCTGTTTCTAACATGAAAGGGGCTAAGCAAGTGGAGGTGAACAGAAAGCAGAGCAAGGTGACGGTGACGGGATACGTGGAGCCAAACAAGGTGTTAAAGAAAGTGAAGAGCACAGGGAAGAAGGCAGAGATTTGGCCGTACGTGCCTTACAACTTGGTGGCATATCCTTATGTCGCCGGAGCTTATGACAAGAAGGCTCCCTCCGGCTACGTGAGGAACGTGACTCAGGCACTTCCGGGCCCGAATGCGCCGGATGACAAGTATACCTCTATGTTTAGCGACGAAAATCCACATGCTTGTTCTATCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

213

Amino Acids

23.94

Weight (kDa)

9.82

Isoelectric Point (pI)

52.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 95 - 150 6.4e-14 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 32
AccI GTMKAC 1 cut(s) 590
AcoI YGGCCR 1 cut(s) 461
AfaI GTAC 1 cut(s) 467
AgsI TTSAA 1 cut(s) 278
AluBI AGCT 1 cut(s) 509
AluI AGCT 1 cut(s) 509
Alw21I GWGCWC 2 cut(s) 208, 440
AoxI GGCC 2 cut(s) 461, 565
ApaI GGGCCC 1 cut(s) 569
ApeKI GCWGC 1 cut(s) 70
AspLEI GCGC 1 cut(s) 577
AspS9I GGNCC 3 cut(s) 6, 565, 566
AsuC2I CCSGG 1 cut(s) 564
AsuHPI GGTGA 4 cut(s) 298, 376, 397, 403
AvaII GGWCC 1 cut(s) 6
BaeGI GKGCMC 1 cut(s) 569
BanII GRGCYC 1 cut(s) 569
Bbv12I GWGCWC 2 cut(s) 208, 440
BbvI GCAGC 1 cut(s) 82
BccI CCATC 1 cut(s) 296
BceAI ACGGC 1 cut(s) 448
BciVI GTATCC 1 cut(s) 392
BcnI CCSGG 1 cut(s) 564
BfaI CTAG 1 cut(s) 149
BfuI GTATCC 1 cut(s) 392
BisI GCNGC 1 cut(s) 71
BlpI GCTNAGC 1 cut(s) 351
BlsI GCNGC 1 cut(s) 72
Bme1390I CCNGG 1 cut(s) 564
Bme18I GGWCC 1 cut(s) 6
BmgT120I GGNCC 3 cut(s) 6, 565, 566
BmiI GGNNCC 3 cut(s) 408, 525, 567
BmrFI CCNGG 1 cut(s) 564
BmsI GCATC 1 cut(s) 202
Bpu1102I GCTNAGC 1 cut(s) 351
BpuEI CTTGAG 1 cut(s) 223
BpuMI CCSGG 1 cut(s) 564
BsaAI YACGTR 3 cut(s) 403, 469, 538
BseGI GGATG 2 cut(s) 217, 586
BseMII CTCAG 2 cut(s) 184, 566
BseRI GAGGAG 1 cut(s) 88
BseSI GKGCMC 1 cut(s) 569
BseXI GCAGC 1 cut(s) 82
BshFI GGCC 2 cut(s) 463, 567
BsiHKAI GWGCWC 2 cut(s) 208, 440
BsiSI CCGG 4 cut(s) 504, 531, 563, 578
BsiWI CGTACG 1 cut(s) 465
BsmI GAATGC 1 cut(s) 577
BsnI GGCC 2 cut(s) 463, 567
Bsp120I GGGCCC 1 cut(s) 565
Bsp1286I GDGCHC 3 cut(s) 208, 440, 569
Bsp143I GATC 1 cut(s) 173
Bsp1720I GCTNAGC 1 cut(s) 351
BspANI GGCC 2 cut(s) 463, 567
BspCNI CTCAG 2 cut(s) 183, 565
BspLI GGNNCC 3 cut(s) 408, 525, 567
BspQI GCTCTTC 1 cut(s) 428
BssMI GATC 1 cut(s) 173
BssNAI GTATAC 1 cut(s) 591
Bst1107I GTATAC 1 cut(s) 591
Bst4CI ACNGT 3 cut(s) 274, 299, 391
Bst6I CTCTTC 1 cut(s) 428
BstBAI YACGTR 3 cut(s) 403, 469, 538
BstDEI CTNAG 3 cut(s) 170, 351, 552
BstF5I GGATG 2 cut(s) 217, 586
BstHHI GCGC 1 cut(s) 577
BstKTI GATC 1 cut(s) 176
BstMBI GATC 1 cut(s) 173
BstMWI GCNNNNNNNGC 3 cut(s) 70, 212, 469
BstNSI RCATGY 1 cut(s) 623
BstSCI CCNGG 1 cut(s) 562
BstSLI GKGCMC 1 cut(s) 569
BstV1I GCAGC 1 cut(s) 82
BstZ17I GTATAC 1 cut(s) 591
BsuI GTATCC 1 cut(s) 392
BsuRI GGCC 2 cut(s) 463, 567
BtsCI GGATG 2 cut(s) 217, 586
BtsI GCAGTG 1 cut(s) 59
BtsIMutI CAGTG 1 cut(s) 59
CfoI GCGC 1 cut(s) 577
Cfr13I GGNCC 3 cut(s) 6, 565, 566
Csp6I GTAC 1 cut(s) 466
CviAII CATG 3 cut(s) 340, 620, 634
CviJI RGCY 9 cut(s) 262, 306, 350, 409, 463, 509, 524, 534, 567
CviKI_1 RGCY 9 cut(s) 262, 306, 350, 409, 463, 509, 524, 534, 567
CviQI GTAC 1 cut(s) 466
DdeI CTNAG 3 cut(s) 170, 351, 552
DpnI GATC 1 cut(s) 175
DpnII GATC 1 cut(s) 173
EaeI YGGCCR 1 cut(s) 461
Eam1104I CTCTTC 1 cut(s) 428
EarI CTCTTC 1 cut(s) 428
Eco24I GRGCYC 1 cut(s) 569
Eco47I GGWCC 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 569
FaeI CATG 3 cut(s) 343, 623, 637
FaiI YATR 9 cut(s) 32, 341, 490, 498, 513, 591, 599, 621, 635
FatI CATG 3 cut(s) 339, 619, 633
FblI GTMKAC 1 cut(s) 590
Fnu4HI GCNGC 1 cut(s) 71
FokI GGATG 2 cut(s) 224, 593
FriOI GRGCYC 1 cut(s) 569
Fsp4HI GCNGC 1 cut(s) 71
FspBI CTAG 1 cut(s) 149
GlaI GCGC 1 cut(s) 576
GluI GCNGC 1 cut(s) 71
HaeIII GGCC 2 cut(s) 463, 567
HapII CCGG 4 cut(s) 504, 531, 563, 578
HhaI GCGC 1 cut(s) 577
Hin1II CATG 3 cut(s) 343, 623, 637
Hin6I GCGC 1 cut(s) 575
HinP1I GCGC 1 cut(s) 575
HinfI GANTC 3 cut(s) 11, 318, 550
HpaII CCGG 4 cut(s) 504, 531, 563, 578
HphI GGTGA 4 cut(s) 298, 376, 397, 403
Hpy166II GTNNAC 2 cut(s) 367, 591
Hpy188I TCNGA 5 cut(s) 27, 173, 178, 187, 223
Hpy188III TCNNGA 2 cut(s) 209, 240
Hpy8I GTNNAC 2 cut(s) 367, 591
Hpy99I CGWCG 1 cut(s) 611
HpyAV CCTTC 2 cut(s) 442, 514
HpyCH4III ACNGT 3 cut(s) 274, 299, 391
HpyCH4IV ACGT 4 cut(s) 402, 468, 537, 546
HpyCH4V TGCA 2 cut(s) 70, 268
HpyF10VI GCNNNNNNNGC 3 cut(s) 70, 212, 469
HpyF3I CTNAG 3 cut(s) 170, 351, 552
HpySE526I ACGT 4 cut(s) 402, 468, 537, 546
Hsp92II CATG 3 cut(s) 343, 623, 637
HspAI GCGC 1 cut(s) 575
Kzo9I GATC 1 cut(s) 173
LguI GCTCTTC 1 cut(s) 428
LmnI GCTCC 3 cut(s) 406, 506, 529
LpnPI CCDG 7 cut(s) 194, 426, 517, 539, 544, 576, 591
Lsp1109I GCAGC 1 cut(s) 82
LweI GCATC 1 cut(s) 202
MaeI CTAG 1 cut(s) 149
MaeII ACGT 4 cut(s) 402, 468, 537, 546
MaeIII GTNAC 4 cut(s) 12, 385, 391, 547
MalI GATC 1 cut(s) 175
MboI GATC 1 cut(s) 173
MboII GAAGA 3 cut(s) 106, 445, 457
MhlI GDGCHC 3 cut(s) 208, 440, 569
MluCI AATT 1 cut(s) 179
MlyI GAGTC 3 cut(s) 20, 327, 544
MnlI CCTC 7 cut(s) 100, 109, 142, 355, 534, 538, 604
MseI TTAA 1 cut(s) 422
MspI CCGG 4 cut(s) 504, 531, 563, 578
MspR9I CCNGG 1 cut(s) 564
Mva1269I GAATGC 1 cut(s) 577
MwoI GCNNNNNNNGC 3 cut(s) 70, 212, 469
NciI CCSGG 1 cut(s) 564
NdeII GATC 1 cut(s) 173
NlaIII CATG 3 cut(s) 343, 623, 637
NlaIV GGNNCC 3 cut(s) 408, 525, 567
NmuCI GTSAC 4 cut(s) 12, 385, 391, 547
NspI RCATGY 1 cut(s) 623
PciSI GCTCTTC 1 cut(s) 428
PcsI WCGNNNNNNNCGW 1 cut(s) 543
PctI GAATGC 1 cut(s) 577
Pfl23II CGTACG 1 cut(s) 465
PflFI GACNNNGTC 1 cut(s) 10
PkrI GCNGC 1 cut(s) 72
PleI GAGTC 3 cut(s) 19, 326, 544
PpsI GAGTC 3 cut(s) 19, 326, 544
Ppu21I YACGTR 3 cut(s) 403, 469, 538
PsiI TTATAA 1 cut(s) 32
PspLI CGTACG 1 cut(s) 465
PspN4I GGNNCC 3 cut(s) 408, 525, 567
PspOMI GGGCCC 1 cut(s) 565
PspPI GGNCC 3 cut(s) 6, 565, 566
PsyI GACNNNGTC 1 cut(s) 10
RsaI GTAC 1 cut(s) 467
RsaNI GTAC 1 cut(s) 466
SapI GCTCTTC 1 cut(s) 428
SaqAI TTAA 1 cut(s) 422
SatI GCNGC 1 cut(s) 71
Sau3AI GATC 1 cut(s) 173
Sau96I GGNCC 3 cut(s) 6, 565, 566
SchI GAGTC 3 cut(s) 20, 327, 544
ScrFI CCNGG 1 cut(s) 564
SduI GDGCHC 3 cut(s) 208, 440, 569
SfaNI GCATC 1 cut(s) 202
SinI GGWCC 1 cut(s) 6
SmlI CTYRAG 1 cut(s) 238
SmoI CTYRAG 1 cut(s) 238
Sse9I AATT 1 cut(s) 179
SspMI CTAG 1 cut(s) 149
StyD4I CCNGG 1 cut(s) 562
TaaI ACNGT 3 cut(s) 274, 299, 391
TaiI ACGT 4 cut(s) 405, 471, 540, 549
TaqII GACCGA 1 cut(s) 23
TasI AATT 1 cut(s) 179
Tru1I TTAA 1 cut(s) 422
Tru9I TTAA 1 cut(s) 422
TscAI CASTG 1 cut(s) 66
TseFI GTSAC 4 cut(s) 12, 385, 391, 547
TseI GCWGC 1 cut(s) 70
Tsp45I GTSAC 4 cut(s) 12, 385, 391, 547
TspDTI ATGAA 1 cut(s) 356
TspRI CASTG 1 cut(s) 66
Tth111I GACNNNGTC 1 cut(s) 10
VpaK11BI GGWCC 1 cut(s) 6
XceI RCATGY 1 cut(s) 623
XmiI GTMKAC 1 cut(s) 590
XspI CTAG 1 cut(s) 149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.