pycom16g04250

Transmembrane Fragile-X-F protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0004923
Physical Location & Seq
Reverse (-)
2657611 .. 2660232
2622 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 726 bp
ATGTTGGTTCAGAGGAGAGTGATGACGTGGCGGTCGGTGGCCAAGTCTCTCCAAACACTTGTCGCCCACGCGTCGCTTTTCACGTTCACGACTCTCCTCGTTCTCAAGCTCCACGGTTCCGTCCGCTACCCTTGGTGGATCATATTTTCCCCTCTCTGGCTGTTCCATGTAGTTGTAGCACGAGGCAGATTTTCGTTACCTGCTCCAAAGCTGCCTAATGATCGCAATTGGGCACCTTTTCATGCTGTCATGGCGACACCGTTGCTTGTCGCATTTGAACTAATGCTTTGTATATACCTTCAGAGCAGATATGTTGTAAGCTTGAAGATTGTCTTTGCACCCTTGTTGGCATTTGAACTAGCAATTCTATTTGATAATGTCAGGATGTGTAAGGCTCTAATGCCTGGAGACGATGAACACGTAAACGATGATGTGATATGGGAAACGCTTCCTCATTTCTGGATTTCTATATCCATGGTCTTCTTTCTTGCTGCCACAGTATTCACTCTTTTAAAGATAAATGGTGATATTTCTGCTCTAGGCTGGTGGGACTTATTCATAAACTTTGGAATTGCACAGGGTTTTGCCTTTCTTGTTTGCACAAAGTGGTATAATCCAGCTATTCACAGACACGCTCACATTCAAGAACCCTGTTCATCTTCAATGAACGTAAGATACCTTGACTGGAATAGTGGCTTAGTCGTTTCTTCAGATGAAGATCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tmemb_185A zf-C3HC4_3

Protein Analysis

242

Amino Acids

27.73

Weight (kDa)

8.39

Isoelectric Point (pI)

34.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tmemb_185A PF10269 35 - 236 2.7e-76 Transmembrane Fragile-X-F protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 31
Acc36I ACCTGC 1 cut(s) 208
AccB1I GGYRCC 1 cut(s) 232
AccII CGCG 1 cut(s) 71
AciI CCGC 2 cut(s) 31, 124
AclWI GGATC 1 cut(s) 146
AcoI YGGCCR 1 cut(s) 39
AcuI CTGAAG 2 cut(s) 284, 693
AdeI CACNNNGTG 1 cut(s) 606
AfiI CCNNNNNNNGG 1 cut(s) 156
AflIII ACRYGT 2 cut(s) 69, 418
AgsI TTSAA 5 cut(s) 278, 325, 356, 644, 663
AjiI CACGTC 1 cut(s) 27
AjnI CCWGG 1 cut(s) 403
AluBI AGCT 4 cut(s) 109, 211, 321, 620
AluI AGCT 4 cut(s) 109, 211, 321, 620
Alw26I GTCTC 2 cut(s) 51, 402
AlwI GGATC 1 cut(s) 146
AoxI GGCC 1 cut(s) 39
ApeKI GCWGC 2 cut(s) 211, 491
Asp700I GAANNNNTTC 1 cut(s) 447
AsuHPI GGTGA 1 cut(s) 536
BaeGI GKGCMC 1 cut(s) 235
BalI TGGCCA 1 cut(s) 41
BanI GGYRCC 1 cut(s) 232
BauI CACGAG 1 cut(s) 180
BbsI GAAGAC 1 cut(s) 472
BbvI GCAGC 2 cut(s) 198, 478
BcgI CGANNNNNNTGC 2 cut(s) 244, 278
BciT130I CCWGG 1 cut(s) 405
BcoDI GTCTC 2 cut(s) 51, 402
BfaI CTAG 2 cut(s) 359, 539
BfuAI ACCTGC 1 cut(s) 208
BisI GCNGC 2 cut(s) 212, 492
BlsI GCNGC 2 cut(s) 213, 493
Bme1390I CCNGG 1 cut(s) 405
BmgBI CACGTC 1 cut(s) 27
BmiI GGNNCC 2 cut(s) 118, 234
BmrFI CCNGG 1 cut(s) 405
BpiI GAAGAC 1 cut(s) 472
BpmI CTGGAG 1 cut(s) 426
BpuEI CTTGAG 1 cut(s) 89
Bsa29I ATCGAT 1 cut(s) 721
BsaAI YACGTR 1 cut(s) 421
BsaBI GATNNNNATC 1 cut(s) 717
BsaJI CCNNGG 3 cut(s) 112, 131, 474
Bsc4I CCNNNNNNNGG 1 cut(s) 156
Bse1I ACTGG 1 cut(s) 689
Bse8I GATNNNNATC 1 cut(s) 717
BseBI CCWGG 1 cut(s) 405
BseCI ATCGAT 1 cut(s) 721
BseDI CCNNGG 3 cut(s) 112, 131, 474
BseGI GGATG 1 cut(s) 390
BseJI GATNNNNATC 1 cut(s) 717
BseLI CCNNNNNNNGG 1 cut(s) 156
BseNI ACTGG 1 cut(s) 689
BseRI GAGGAG 2 cut(s) 28, 86
BseSI GKGCMC 1 cut(s) 235
BseXI GCAGC 2 cut(s) 198, 478
Bsh1236I CGCG 1 cut(s) 71
Bsh1285I CGRYCG 1 cut(s) 35
BshFI GGCC 1 cut(s) 41
BshNI GGYRCC 1 cut(s) 232
BshVI ATCGAT 1 cut(s) 721
BsiEI CGRYCG 1 cut(s) 35
BslFI GGGAC 1 cut(s) 563
BslI CCNNNNNNNGG 1 cut(s) 156
BsmAI GTCTC 2 cut(s) 51, 402
BsmBI CGTCTC 1 cut(s) 402
BsmFI GGGAC 1 cut(s) 563
BsnI GGCC 1 cut(s) 41
Bsp1286I GDGCHC 1 cut(s) 235
Bsp143I GATC 3 cut(s) 138, 220, 718
Bsp19I CCATGG 1 cut(s) 474
BspACI CCGC 2 cut(s) 31, 124
BspANI GGCC 1 cut(s) 41
BspDI ATCGAT 1 cut(s) 721
BspFNI CGCG 1 cut(s) 71
BspLI GGNNCC 2 cut(s) 118, 234
BspMI ACCTGC 1 cut(s) 208
BspPI GGATC 1 cut(s) 146
BspT107I GGYRCC 1 cut(s) 232
BsrI ACTGG 1 cut(s) 689
BssECI CCNNGG 3 cut(s) 112, 131, 474
BssMI GATC 3 cut(s) 138, 220, 718
BssSI CACGAG 1 cut(s) 180
BssT1I CCWWGG 2 cut(s) 131, 474
Bst2BI CACGAG 1 cut(s) 180
Bst2UI CCWGG 1 cut(s) 405
Bst4CI ACNGT 3 cut(s) 116, 261, 499
BstBAI YACGTR 1 cut(s) 421
BstDEI CTNAG 1 cut(s) 697
BstDSI CCRYGG 2 cut(s) 112, 474
BstF5I GGATG 1 cut(s) 390
BstFNI CGCG 1 cut(s) 71
BstKTI GATC 3 cut(s) 141, 223, 721
BstMAI GTCTC 2 cut(s) 51, 402
BstMBI GATC 3 cut(s) 138, 220, 718
BstMCI CGRYCG 1 cut(s) 35
BstMWI GCNNNNNNNGC 1 cut(s) 251
BstNI CCWGG 1 cut(s) 405
BstSCI CCNGG 1 cut(s) 403
BstSLI GKGCMC 1 cut(s) 235
BstUI CGCG 1 cut(s) 71
BstV1I GCAGC 2 cut(s) 198, 478
BstV2I GAAGAC 1 cut(s) 472
Bsu15I ATCGAT 1 cut(s) 721
BsuRI GGCC 1 cut(s) 41
BsuTUI ATCGAT 1 cut(s) 721
BtgI CCRYGG 2 cut(s) 112, 474
BtrI CACGTC 1 cut(s) 27
BtsCI GGATG 1 cut(s) 390
BveI ACCTGC 1 cut(s) 208
ClaI ATCGAT 1 cut(s) 721
CseI GACGC 1 cut(s) 60
CviAII CATG 4 cut(s) 167, 242, 250, 475
CviJI RGCY 9 cut(s) 41, 109, 160, 211, 321, 395, 543, 620, 696
CviKI_1 RGCY 9 cut(s) 41, 109, 160, 211, 321, 395, 543, 620, 696
DdeI CTNAG 1 cut(s) 697
DpnI GATC 3 cut(s) 140, 222, 720
DpnII GATC 3 cut(s) 138, 220, 718
DraI TTTAAA 1 cut(s) 513
DraIII CACNNNGTG 1 cut(s) 606
DrdI GACNNNNNNGTC 1 cut(s) 31
DseDI GACNNNNNNGTC 1 cut(s) 31
EaeI YGGCCR 1 cut(s) 39
Eco130I CCWWGG 2 cut(s) 131, 474
Eco57I CTGAAG 2 cut(s) 284, 693
EcoRII CCWGG 1 cut(s) 403
EcoT14I CCWWGG 2 cut(s) 131, 474
ErhI CCWWGG 2 cut(s) 131, 474
Esp3I CGTCTC 1 cut(s) 402
FaeI CATG 4 cut(s) 170, 245, 253, 478
FalI AAGNNNNNCTT 2 cut(s) 317, 349
FaqI GGGAC 1 cut(s) 563
FatI CATG 4 cut(s) 166, 241, 249, 474
Fnu4HI GCNGC 2 cut(s) 212, 492
FokI GGATG 1 cut(s) 397
Fsp4HI GCNGC 2 cut(s) 212, 492
FspBI CTAG 2 cut(s) 359, 539
GluI GCNGC 2 cut(s) 212, 492
GsuI CTGGAG 1 cut(s) 426
HaeIII GGCC 1 cut(s) 41
HgaI GACGC 1 cut(s) 60
Hin1II CATG 4 cut(s) 170, 245, 253, 478
HindIII AAGCTT 1 cut(s) 319
HinfI GANTC 1 cut(s) 91
HphI GGTGA 1 cut(s) 536
Hpy166II GTNNAC 2 cut(s) 87, 424
Hpy188I TCNGA 3 cut(s) 12, 303, 712
Hpy188III TCNNGA 4 cut(s) 88, 382, 460, 644
Hpy8I GTNNAC 2 cut(s) 87, 424
Hpy99I CGWCG 1 cut(s) 76
HpyAV CCTTC 1 cut(s) 308
HpyCH4III ACNGT 3 cut(s) 116, 261, 499
HpyCH4IV ACGT 4 cut(s) 26, 83, 420, 669
HpyCH4V TGCA 3 cut(s) 338, 575, 600
HpyF10VI GCNNNNNNNGC 1 cut(s) 251
HpyF3I CTNAG 1 cut(s) 697
HpySE526I ACGT 4 cut(s) 26, 83, 420, 669
Hsp92II CATG 4 cut(s) 170, 245, 253, 478
Kzo9I GATC 3 cut(s) 138, 220, 718
LmnI GCTCC 2 cut(s) 114, 208
Lsp1109I GCAGC 2 cut(s) 198, 478
MaeI CTAG 2 cut(s) 359, 539
MaeII ACGT 4 cut(s) 26, 83, 420, 669
MaeIII GTNAC 1 cut(s) 195
MalI GATC 3 cut(s) 140, 222, 720
MboI GATC 3 cut(s) 138, 220, 718
MboII GAAGA 4 cut(s) 337, 472, 651, 699
MfeI CAATTG 1 cut(s) 226
MhlI GDGCHC 1 cut(s) 235
MlsI TGGCCA 1 cut(s) 41
MluCI AATT 3 cut(s) 226, 363, 570
MluI ACGCGT 1 cut(s) 69
MluNI TGGCCA 1 cut(s) 41
MlyI GAGTC 1 cut(s) 85
MnlI CCTC 5 cut(s) 6, 107, 162, 176, 462
Mox20I TGGCCA 1 cut(s) 41
MroXI GAANNNNTTC 1 cut(s) 447
MscI TGGCCA 1 cut(s) 41
MseI TTAA 1 cut(s) 512
Msp20I TGGCCA 1 cut(s) 41
MspR9I CCNGG 1 cut(s) 405
MunI CAATTG 1 cut(s) 226
MvaI CCWGG 1 cut(s) 405
MvnI CGCG 1 cut(s) 71
MwoI GCNNNNNNNGC 1 cut(s) 251
NcoI CCATGG 1 cut(s) 474
NdeII GATC 3 cut(s) 138, 220, 718
NlaIII CATG 4 cut(s) 170, 245, 253, 478
NlaIV GGNNCC 2 cut(s) 118, 234
PcsI WCGNNNNNNNCGW 2 cut(s) 80, 417
PdmI GAANNNNTTC 1 cut(s) 447
PkrI GCNGC 2 cut(s) 213, 493
PleI GAGTC 1 cut(s) 85
PpsI GAGTC 1 cut(s) 85
Ppu21I YACGTR 1 cut(s) 421
Psp6I CCWGG 1 cut(s) 403
PspGI CCWGG 1 cut(s) 403
PspN4I GGNNCC 2 cut(s) 118, 234
PsrI GAACNNNNNNTAC 2 cut(s) 659, 691
SaqAI TTAA 1 cut(s) 512
SatI GCNGC 2 cut(s) 212, 492
Sau3AI GATC 3 cut(s) 138, 220, 718
SchI GAGTC 1 cut(s) 85
ScrFI CCNGG 1 cut(s) 405
SduI GDGCHC 1 cut(s) 235
SmlI CTYRAG 1 cut(s) 104
SmoI CTYRAG 1 cut(s) 104
Sse9I AATT 3 cut(s) 226, 363, 570
SsiI CCGC 2 cut(s) 31, 124
SspMI CTAG 2 cut(s) 359, 539
StyD4I CCNGG 1 cut(s) 403
StyI CCWWGG 2 cut(s) 131, 474
TaaI ACNGT 3 cut(s) 116, 261, 499
TaiI ACGT 4 cut(s) 29, 86, 423, 672
TaqI TCGA 1 cut(s) 721
TasI AATT 3 cut(s) 226, 363, 570
Tru1I TTAA 1 cut(s) 512
Tru9I TTAA 1 cut(s) 512
TseI GCWGC 2 cut(s) 211, 491
TspDTI ATGAA 5 cut(s) 230, 429, 547, 645, 680
TspGWI ACGGA 1 cut(s) 109
XmnI GAANNNNTTC 1 cut(s) 447
XspI CTAG 2 cut(s) 359, 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.