Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0004209

Physical Location & Seq

Genomic Coordinates Reverse (-)

3417163 .. 3420759

Gene Structure 3597 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1983 bp

ATGTGCGGAATCGCTTTGATCATCTGTGGCATTCGTTTTGATCTGTCATCCCTGCTTCTCAATTCCACATCTGCACTCCCCAACTCCGACCAACTTCCGTTCGCCATTGACGACCTCAAAGAAGCTTTGCGGAGGAGGGGTCCCGATAGCTTAGGCAGCACGAACCTTCTTCTCCGTTCAGCCGTTTCGAATCGTCTCCCGGAGCGAGAAATTGTGTCTTCCATTGAGGGGGAAATAAAGGAGTCTGGTTGCGAAGGCGATGAAGGCAATTGTTTCTCTTTGGCAAATGGTCGAACACATCTGCATACACATTTCAGCACCGTTCCCAGTTCGTCTGCTGAGTTGCACTTGCTGGGTGCCACTTTGCAGCTCAGGGGAGTAAGCTCTGTGGTTCAGCCATTGATAGATTCGGATAAGAATATCCTTGTTTATAATGGTGAAATCTTTGGGGGCATAGACATTGGTAGAGATGAGAACGATGGCGAAGTTCTTCTGCAGTTGCTGGGAGAGTGCTGTTCGATTTCTGTTCCAGATGTTCTTTCTAAGATCAAAGGGCCTTGGGCTATCATCTATTGGCAGGAAAGTTCAAAAACCCTGTGGTTTGCTCGAGATGCATTTGGTCGGCGGAGTCTTCTTGTTCACTGGCCAACACTGGAGGACTCTCGGTTTCTGCTGTCGTCTGTATCACCTTTTTCTTCAAATGAGCGTTCTTTTGATTTTGAAGCTGAAAATGGAACTACCAAGCTGAATTTTTGGGAAGAGCTCCGATGTGGAATATACAGTATATCTATGAATGCTCCAGGTCTTGATGGGATTTTGGTTGGTGAAGTGCAAAAACATGAGTGGACAAATGGTGTACTAGAAGAACTGATAAAATGGGAGAGAACTTCTGTCGAACCTAAACATGAAGACTTACATATTTCCTGTTCCAAGACTCTGACAGGGAAACGTGATATGCAGTCAGTTAATTCAGATACAGAACCATCTAAATCAGGATCTATACAAGCTTCAATTTCTGTGCCAGCACAGGCGGTACTGATTGCTTTAAGGGAATCGGTTAGGCGGCGCTCTTCACTGCACACAATTTTTCAGGCAGGTAGACATGATATGGAAAAGGAATTTGTACCGGTGGCAGTTCTTTTTTCTGGTGGATTGGATTCTATGATAATTGCAGCCTTGTTACATGAGTGCCTTGATCCGAGCTATGATATTGATCTACTTAATGTGAGCTTTGATGGTCGGTCTGCTCCTGACAGAATCTCTGCCAGGGCAGGAGTATGCGAACTGAGAAGAATTGCACCTTCAAGAAAGTGGAAGCTTGTGGAGATCGACGCTGAATTGTCAACATTGACCTTTGAAACAAAGCATGTCATGTCGCTCATAAATCCTGCTAACACCTACATGGACCTGAATATTGGAATTGCCTTGTGGCTTGCTGCTGCTGGTGATGGATGGGTATATGAGGAAAATACCGATTATAATGATGAGGATTGTCAATGCATCAAGTACAAGTCCAAGGCTAGGATTCTTCTTGTTGGTTCAGGTGCAGATGAGCAATGCGCTGGCTATGGTAGGCACAGAACGAAATATAGAAATGGCAGCTGGCGTGCACTAAACGATGAAATGAAATTGGACATGCAGAGAATTTGGAAAAGAAACCTTGGGAGAGATGATAGGTGCATTGCTGACAATGGCAAGGAGGCAAGGTTTCCGTTCCTGGATGAAGATGTTATAAGAATTTTGCTTGGGTTTCCTCTCTGGGAGGTTGCCAGCCTTGATCAACCGAGCGGGATAGGAGATAAGAAGATTCTGAGAGGGGTCGCAGAATTGCTTGGTTTGTATGAAGCTGCTTCTCTGCCTAAAAGAGCTATACAGTTTGGTTCAAGAATTGCAAGGGAATCAAACCGAAAGAATTACGGCAGTAACCGTGCAGCAAATCAAGCATCTGCAGGAAGTGCAGTGATTCACAAAAGGTTGGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004066 GO:0005488 GO:0005515 GO:0006082 GO:0006520 GO:0006528 GO:0006529 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009066 GO:0009067 GO:0009987 GO:0016053 GO:0016874 GO:0016879 GO:0016884 GO:0019752 GO:0032787 GO:0034641 GO:0042802 GO:0042803 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0046394 GO:0046983 GO:0071704 GO:0072330 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Asn_synthase GATase_7

Protein Analysis

661

Amino Acids

73.19

Weight (kDa)

5.61

Isoelectric Point (pI)

44.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GATase_6	PF13522	116 - 216	4.1e-07	Glutamine amidotransferase domain
GATase_7	PF13537	128 - 227	8.9e-10	Glutamine amidotransferase domain
Asn_synthase	PF00733	533 - 608	5e-06	Asparagine synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004209)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G03667 AT2G03667
fragaria_vesca	FvH4_4g29880 FvH4_4g29880 FvH4_4g29880 FvH4_4g29880 FvH4_4g29880 FvH4_4g29880 FvH4_4g29880 FvH4_4g29880
malus_domestica	MD13G1061700.v1.1 MD16G1060200.v1.1
prunus_persica	Prupe.1G288300_v2.0.a1 Prupe.1G288300_v2.0.a1 Prupe.1G288300_v2.0.a1 Prupe.1G288300_v2.0.a1
pyrus_communis	pycom16g05240
rosa_chinensis	RchiOBHm_Chr4g0438141
rosa_laevigata	RLG00000006385
rosa_multiflora	Rmu_sc0004728.1_g000004 Rmu_sc0020161.1_g000004
rosa_roxburghii	Rroxscaffold_5G00379270
rosa_rugosa	Rorug04G0307300 Rorug04G0307400 Rorug04G0307500 Rorug04G0307600 Rorug04G0307700
rosa_samantha	Rh4AG358800 Rh4BG369700 Rh4CG383800 Rh4DG364600
rosa_wichuraiana	Rw0G006330 Rw0G016270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	432, 1479, 1733
Acc36I	ACCTGC	1 cut(s)	1085
AccB1I	GGYRCC	1 cut(s)	356
AccBSI	CCGCTC	1 cut(s)	1788
AccI	GTMKAC	1 cut(s)	1099
AciI	CCGC	6 cut(s)	6, 130, 625, 1031, 1063, 1788
AclWI	GGATC	2 cut(s)	1003, 1190
AcoI	YGGCCR	1 cut(s)	644
AcsI	RAATTY	4 cut(s)	748, 1118, 1644, 1737
AfaI	GTAC	4 cut(s)	858, 1035, 1125, 1508
AfiI	CCNNNNNNNGG	2 cut(s)	228, 1521
AgeI	ACCGGT	1 cut(s)	1126
AgsI	TTSAA	7 cut(s)	588, 699, 722, 1011, 1305, 1358, 1884
AjnI	CCWGG	3 cut(s)	799, 1265, 1716
Alw21I	GWGCWC	2 cut(s)	765, 1612
Alw26I	GTCTC	1 cut(s)	200
Alw44I	GTGCAC	1 cut(s)	1608
AlwI	GGATC	2 cut(s)	1003, 1190
AlwNI	CAGNNNCTG	1 cut(s)	502
Ama87I	CYCGRG	1 cut(s)	606
AoxI	GGCC	2 cut(s)	554, 644
ApaLI	GTGCAC	1 cut(s)	1608
ApeKI	GCWGC	8 cut(s)	156, 367, 1172, 1436, 1439, 1599, 1847, 1931
ApoI	RAATTY	4 cut(s)	748, 1118, 1644, 1737
AsiGI	ACCGGT	1 cut(s)	1126
Asp700I	GAANNNNTTC	1 cut(s)	489
AspLEI	GCGC	2 cut(s)	1068, 1562
AspS9I	GGNCC	3 cut(s)	140, 554, 1405
AsuC2I	CCSGG	1 cut(s)	200
AsuHPI	GGTGA	4 cut(s)	449, 678, 836, 1457
AsuII	TTCGAA	1 cut(s)	188
AvaI	CYCGRG	1 cut(s)	606
AvaII	GGWCC	2 cut(s)	140, 1405
BaeGI	GKGCMC	1 cut(s)	1612
BalI	TGGCCA	1 cut(s)	646
BanI	GGYRCC	1 cut(s)	356
BanII	GRGCYC	1 cut(s)	765
BbsI	GAAGAC	3 cut(s)	210, 623, 915
Bbv12I	GWGCWC	2 cut(s)	765, 1612
BbvI	GCAGC	8 cut(s)	168, 379, 1184, 1423, 1426, 1611, 1834, 1943
BccI	CCATC	6 cut(s)	473, 803, 991, 1229, 1442, 1446
BceAI	ACGGC	2 cut(s)	167, 1933
BciT130I	CCWGG	3 cut(s)	801, 1267, 1718
BclI	TGATCA	2 cut(s)	18, 1777
BcnI	CCSGG	1 cut(s)	200
BcoDI	GTCTC	1 cut(s)	200
BfaI	CTAG	2 cut(s)	860, 1521
BfmI	CTRYAG	2 cut(s)	494, 1947
BfoI	RGCGCY	1 cut(s)	1069
BfuAI	ACCTGC	1 cut(s)	1085
BisI	GCNGC	9 cut(s)	157, 368, 1064, 1173, 1437, 1440, 1600, 1848, 1932
BlsI	GCNGC	9 cut(s)	158, 369, 1065, 1174, 1438, 1441, 1601, 1849, 1933
Bme1390I	CCNGG	4 cut(s)	200, 801, 1267, 1718
Bme18I	GGWCC	2 cut(s)	140, 1405
BmeT110I	CYCGRG	1 cut(s)	606
BmgT120I	GGNCC	3 cut(s)	140, 554, 1405
BmiI	GGNNCC	3 cut(s)	141, 142, 358
BmrFI	CCNGG	4 cut(s)	200, 801, 1267, 1718
BmrI	ACTGGG	1 cut(s)	321
BmsI	GCATC	3 cut(s)	601, 1509, 1952
BmuI	ACTGGG	1 cut(s)	321
BpiI	GAAGAC	3 cut(s)	210, 623, 915
BpmI	CTGGAG	2 cut(s)	674, 783
Bpu10I	CCTNAGC	2 cut(s)	151, 371
Bpu14I	TTCGAA	1 cut(s)	188
BpuMI	CCSGG	1 cut(s)	200
BsaBI	GATNNNNATC	1 cut(s)	1212
BsaJI	CCNNGG	4 cut(s)	557, 1266, 1515, 1660
BsaWI	WCCGGW	1 cut(s)	1126
BsaXI	ACNNNNNCTCC	4 cut(s)	124, 154, 1691, 1721
Bsc4I	CCNNNNNNNGG	2 cut(s)	228, 1521
Bse118I	RCCGGY	1 cut(s)	1126
Bse1I	ACTGG	3 cut(s)	327, 647, 657
Bse3DI	GCAATG	2 cut(s)	1562, 1680
Bse8I	GATNNNNATC	1 cut(s)	1212
BseBI	CCWGG	3 cut(s)	801, 1267, 1718
BseDI	CCNNGG	4 cut(s)	557, 1266, 1515, 1660
BseGI	GGATG	3 cut(s)	47, 1457, 1726
BseJI	GATNNNNATC	1 cut(s)	1212
BseLI	CCNNNNNNNGG	2 cut(s)	228, 1521
BseMI	GCAATG	2 cut(s)	1562, 1680
BseMII	CTCAG	4 cut(s)	330, 385, 1277, 1802
BseNI	ACTGG	3 cut(s)	327, 647, 657
BseRI	GAGGAG	1 cut(s)	148
BseSI	GKGCMC	1 cut(s)	1612
BseXI	GCAGC	8 cut(s)	168, 379, 1184, 1423, 1426, 1611, 1834, 1943
BseYI	CCCAGC	2 cut(s)	352, 502
BsgI	GTGCAG	5 cut(s)	57, 1061, 1566, 1950, 1977
BshFI	GGCC	2 cut(s)	556, 646
BshNI	GGYRCC	1 cut(s)	356
BshTI	ACCGGT	1 cut(s)	1126
BsiHKAI	GWGCWC	2 cut(s)	765, 1612
BsiHKCI	CYCGRG	1 cut(s)	606
BsiSI	CCGG	2 cut(s)	200, 1127
BslFI	GGGAC	1 cut(s)	126
BslI	CCNNNNNNNGG	2 cut(s)	228, 1521
BsmAI	GTCTC	1 cut(s)	200
BsmBI	CGTCTC	1 cut(s)	200
BsmFI	GGGAC	1 cut(s)	126
BsmI	GAATGC	2 cut(s)	30, 799
BsnI	GGCC	2 cut(s)	556, 646
BsoBI	CYCGRG	1 cut(s)	606
Bsp119I	TTCGAA	1 cut(s)	188
Bsp1286I	GDGCHC	2 cut(s)	765, 1612
Bsp143I	GATC	8 cut(s)	18, 40, 546, 995, 1195, 1213, 1326, 1777
BspACI	CCGC	6 cut(s)	6, 130, 625, 1031, 1063, 1788
BspANI	GGCC	2 cut(s)	556, 646
BspCNI	CTCAG	4 cut(s)	331, 384, 1278, 1803
BspLI	GGNNCC	3 cut(s)	141, 142, 358
BspMAI	CTGCAG	2 cut(s)	498, 1951
BspMI	ACCTGC	1 cut(s)	1085
BspPI	GGATC	2 cut(s)	1003, 1190
BspQI	GCTCTTC	2 cut(s)	753, 1075
BspT104I	TTCGAA	1 cut(s)	188
BspT107I	GGYRCC	1 cut(s)	356
BsrBI	CCGCTC	1 cut(s)	1788
BsrDI	GCAATG	2 cut(s)	1562, 1680
BsrFI	RCCGGY	1 cut(s)	1126
BsrI	ACTGG	3 cut(s)	327, 647, 657
BssAI	RCCGGY	1 cut(s)	1126
BssECI	CCNNGG	4 cut(s)	557, 1266, 1515, 1660
BssMI	GATC	8 cut(s)	18, 40, 546, 995, 1195, 1213, 1326, 1777
BssT1I	CCWWGG	3 cut(s)	557, 1515, 1660
Bst2UI	CCWGG	3 cut(s)	801, 1267, 1718
Bst4CI	ACNGT	4 cut(s)	322, 782, 1875, 1928
Bst6I	CTCTTC	2 cut(s)	753, 1075
BstAPI	GCANNNNNTGC	1 cut(s)	1955
BstBI	TTCGAA	1 cut(s)	188
BstC8I	GCNNGC	6 cut(s)	1023, 1434, 1564, 1604, 1608, 1771
BstDEI	CTNAG	6 cut(s)	151, 339, 371, 543, 1286, 1811
BstF5I	GGATG	3 cut(s)	47, 1457, 1726
BstH2I	RGCGCY	1 cut(s)	1069
BstHHI	GCGC	2 cut(s)	1068, 1562
BstKTI	GATC	8 cut(s)	21, 43, 549, 998, 1198, 1216, 1329, 1780
BstMAI	GTCTC	1 cut(s)	200
BstMBI	GATC	8 cut(s)	18, 40, 546, 995, 1195, 1213, 1326, 1777
BstMWI	GCNNNNNNNGC	5 cut(s)	156, 264, 611, 1940, 1955
BstNI	CCWGG	3 cut(s)	801, 1267, 1718
BstNSI	RCATGY	2 cut(s)	1370, 1639
BstSCI	CCNGG	4 cut(s)	198, 799, 1265, 1716
BstSFI	CTRYAG	2 cut(s)	494, 1947
BstSLI	GKGCMC	1 cut(s)	1612
BstV1I	GCAGC	8 cut(s)	168, 379, 1184, 1423, 1426, 1611, 1834, 1943
BstV2I	GAAGAC	3 cut(s)	210, 623, 915
BstX2I	RGATCY	1 cut(s)	995
BstYI	RGATCY	1 cut(s)	995
BsuRI	GGCC	2 cut(s)	556, 646
BtgZI	GCGATG	1 cut(s)	273
BtsCI	GGATG	3 cut(s)	47, 1457, 1726
BtsI	GCAGTG	2 cut(s)	1073, 1965
BtsIMutI	CAGTG	4 cut(s)	640, 650, 1073, 1965
BveI	ACCTGC	1 cut(s)	1085
Cac8I	GCNNGC	6 cut(s)	1023, 1434, 1564, 1604, 1608, 1771
CaiI	CAGNNNCTG	1 cut(s)	502
CfoI	GCGC	2 cut(s)	1068, 1562
Cfr10I	RCCGGY	1 cut(s)	1126
Cfr13I	GGNCC	3 cut(s)	140, 554, 1405
CseI	GACGC	1 cut(s)	1340
Csp6I	GTAC	4 cut(s)	857, 1034, 1124, 1507
CspAI	ACCGGT	1 cut(s)	1126
CviAII	CATG	8 cut(s)	839, 905, 1103, 1184, 1367, 1372, 1402, 1636
CviQI	GTAC	4 cut(s)	857, 1034, 1124, 1507
DdeI	CTNAG	6 cut(s)	151, 339, 371, 543, 1286, 1811
DpnI	GATC	8 cut(s)	20, 42, 548, 997, 1197, 1215, 1328, 1779
DpnII	GATC	8 cut(s)	18, 40, 546, 995, 1195, 1213, 1326, 1777
EaeI	YGGCCR	1 cut(s)	644
Eam1104I	CTCTTC	2 cut(s)	753, 1075
EarI	CTCTTC	2 cut(s)	753, 1075
EciI	GGCGGA	1 cut(s)	640
Ecl136II	GAGCTC	1 cut(s)	763
Eco130I	CCWWGG	3 cut(s)	557, 1515, 1660
Eco24I	GRGCYC	1 cut(s)	765
Eco47I	GGWCC	2 cut(s)	140, 1405
Eco53kI	GAGCTC	1 cut(s)	763
Eco88I	CYCGRG	1 cut(s)	606
EcoICRI	GAGCTC	1 cut(s)	763
EcoO109I	RGGNCCY	2 cut(s)	140, 554
EcoRII	CCWGG	3 cut(s)	799, 1265, 1716
EcoT14I	CCWWGG	3 cut(s)	557, 1515, 1660
EcoT22I	ATGCAT	2 cut(s)	616, 1502
EcoT38I	GRGCYC	1 cut(s)	765
ErhI	CCWWGG	3 cut(s)	557, 1515, 1660
Esp3I	CGTCTC	1 cut(s)	200
FaeI	CATG	8 cut(s)	842, 908, 1106, 1187, 1370, 1375, 1405, 1639
FaqI	GGGAC	1 cut(s)	126
FatI	CATG	8 cut(s)	838, 904, 1102, 1183, 1366, 1371, 1401, 1635
FauI	CCCGC	1 cut(s)	1781
FbaI	TGATCA	2 cut(s)	18, 1777
FblI	GTMKAC	1 cut(s)	1099
Fnu4HI	GCNGC	9 cut(s)	157, 368, 1064, 1173, 1437, 1440, 1600, 1848, 1932
FokI	GGATG	3 cut(s)	34, 1464, 1733
FriOI	GRGCYC	1 cut(s)	765
Fsp4HI	GCNGC	9 cut(s)	157, 368, 1064, 1173, 1437, 1440, 1600, 1848, 1932
FspBI	CTAG	2 cut(s)	860, 1521
GlaI	GCGC	2 cut(s)	1067, 1561
GluI	GCNGC	9 cut(s)	157, 368, 1064, 1173, 1437, 1440, 1600, 1848, 1932
GsaI	CCCAGC	2 cut(s)	356, 506
GsuI	CTGGAG	2 cut(s)	674, 783
HaeII	RGCGCY	1 cut(s)	1069
HaeIII	GGCC	2 cut(s)	556, 646
HapII	CCGG	2 cut(s)	200, 1127
HgaI	GACGC	1 cut(s)	1340
HhaI	GCGC	2 cut(s)	1068, 1562
Hin1II	CATG	8 cut(s)	842, 908, 1106, 1187, 1370, 1375, 1405, 1639
Hin6I	GCGC	2 cut(s)	1066, 1560
HinP1I	GCGC	2 cut(s)	1066, 1560
HincII	GTYRAC	1 cut(s)	1344
HindII	GTYRAC	1 cut(s)	1344
HindIII	AAGCTT	3 cut(s)	123, 1005, 1316
HpaII	CCGG	2 cut(s)	200, 1127
HphI	GGTGA	4 cut(s)	449, 678, 836, 1457
Hpy166II	GTNNAC	6 cut(s)	640, 846, 857, 1100, 1344, 1610
Hpy188I	TCNGA	7 cut(s)	88, 412, 767, 939, 973, 1200, 1812
Hpy188III	TCNNGA	8 cut(s)	143, 530, 608, 806, 993, 1250, 1305, 1884
Hpy8I	GTNNAC	6 cut(s)	640, 846, 857, 1100, 1344, 1610
Hpy99I	CGWCG	1 cut(s)	1334
HpyAV	CCTTC	4 cut(s)	176, 248, 257, 1311
HpyCH4III	ACNGT	4 cut(s)	322, 782, 1875, 1928
HpyCH4IV	ACGT	1 cut(s)	949
HpyF10VI	GCNNNNNNNGC	5 cut(s)	156, 264, 611, 1940, 1955
HpyF3I	CTNAG	6 cut(s)	151, 339, 371, 543, 1286, 1811
HpySE526I	ACGT	1 cut(s)	949
Hsp92II	CATG	8 cut(s)	842, 908, 1106, 1187, 1370, 1375, 1405, 1639
HspAI	GCGC	2 cut(s)	1066, 1560
KflI	GGGWCCC	1 cut(s)	140
Ksp22I	TGATCA	2 cut(s)	18, 1777
Kzo9I	GATC	8 cut(s)	18, 40, 546, 995, 1195, 1213, 1326, 1777
LguI	GCTCTTC	2 cut(s)	753, 1075
LmnI	GCTCC	4 cut(s)	202, 768, 802, 1252
Lsp1109I	GCAGC	8 cut(s)	168, 379, 1184, 1423, 1426, 1611, 1834, 1943
LweI	GCATC	3 cut(s)	601, 1509, 1952
MaeI	CTAG	2 cut(s)	860, 1521
MaeII	ACGT	1 cut(s)	949
MaeIII	GTNAC	2 cut(s)	1179, 1922
MalI	GATC	8 cut(s)	20, 42, 548, 997, 1197, 1215, 1328, 1779
MbiI	CCGCTC	1 cut(s)	1788
MboI	GATC	8 cut(s)	18, 40, 546, 995, 1195, 1213, 1326, 1777
MfeI	CAATTG	1 cut(s)	268
MflI	RGATCY	1 cut(s)	995
MhlI	GDGCHC	2 cut(s)	765, 1612
MlsI	TGGCCA	1 cut(s)	646
MluNI	TGGCCA	1 cut(s)	646
MlyI	GAGTC	4 cut(s)	251, 637, 653, 928
MmeI	TCCRAC	2 cut(s)	111, 1956
Mox20I	TGGCCA	1 cut(s)	646
Mph1103I	ATGCAT	2 cut(s)	616, 1502
MroXI	GAANNNNTTC	1 cut(s)	489
MscI	TGGCCA	1 cut(s)	646
MseI	TTAA	3 cut(s)	966, 1046, 1221
MslI	CAYNNNNRTG	1 cut(s)	1400
Msp20I	TGGCCA	1 cut(s)	646
MspA1I	CMGCKG	1 cut(s)	1602
MspI	CCGG	2 cut(s)	200, 1127
MspR9I	CCNGG	4 cut(s)	200, 801, 1267, 1718
MunI	CAATTG	1 cut(s)	268
Mva1269I	GAATGC	2 cut(s)	30, 799
MvaI	CCWGG	3 cut(s)	801, 1267, 1718
MwoI	GCNNNNNNNGC	5 cut(s)	156, 264, 611, 1940, 1955
NciI	CCSGG	1 cut(s)	200
NdeII	GATC	8 cut(s)	18, 40, 546, 995, 1195, 1213, 1326, 1777
NlaIII	CATG	8 cut(s)	842, 908, 1106, 1187, 1370, 1375, 1405, 1639
NlaIV	GGNNCC	3 cut(s)	141, 142, 358
NsiI	ATGCAT	2 cut(s)	616, 1502
NspI	RCATGY	2 cut(s)	1370, 1639
NspV	TTCGAA	1 cut(s)	188
PaeR7I	CTCGAG	1 cut(s)	606
PciSI	GCTCTTC	2 cut(s)	753, 1075
PcsI	WCGNNNNNNNCGW	1 cut(s)	108
PctI	GAATGC	2 cut(s)	30, 799
PdmI	GAANNNNTTC	1 cut(s)	489
PfoI	TCCNGGA	2 cut(s)	198, 1716
PinAI	ACCGGT	1 cut(s)	1126
PkrI	GCNGC	9 cut(s)	158, 369, 1065, 1174, 1438, 1441, 1601, 1849, 1933
PleI	GAGTC	4 cut(s)	250, 636, 653, 928
PpsI	GAGTC	4 cut(s)	250, 636, 653, 928
PpuMI	RGGWCCY	1 cut(s)	140
PsiI	TTATAA	3 cut(s)	432, 1479, 1733
Psp124BI	GAGCTC	1 cut(s)	765
Psp5II	RGGWCCY	1 cut(s)	140
Psp6I	CCWGG	3 cut(s)	799, 1265, 1716
PspFI	CCCAGC	2 cut(s)	352, 502
PspGI	CCWGG	3 cut(s)	799, 1265, 1716
PspN4I	GGNNCC	3 cut(s)	141, 142, 358
PspPI	GGNCC	3 cut(s)	140, 554, 1405
PspPPI	RGGWCCY	1 cut(s)	140
PstI	CTGCAG	2 cut(s)	498, 1951
PstNI	CAGNNNCTG	1 cut(s)	502
PsuI	RGATCY	1 cut(s)	995
PvuII	CAGCTG	1 cut(s)	1602
RsaI	GTAC	4 cut(s)	858, 1035, 1125, 1508
RsaNI	GTAC	4 cut(s)	857, 1034, 1124, 1507
RseI	CAYNNNNRTG	1 cut(s)	1400
SacI	GAGCTC	1 cut(s)	765
SapI	GCTCTTC	2 cut(s)	753, 1075
SaqAI	TTAA	3 cut(s)	966, 1046, 1221
SatI	GCNGC	9 cut(s)	157, 368, 1064, 1173, 1437, 1440, 1600, 1848, 1932
Sau3AI	GATC	8 cut(s)	18, 40, 546, 995, 1195, 1213, 1326, 1777
Sau96I	GGNCC	3 cut(s)	140, 554, 1405
SchI	GAGTC	4 cut(s)	251, 637, 653, 928
ScrFI	CCNGG	4 cut(s)	200, 801, 1267, 1718
SduI	GDGCHC	2 cut(s)	765, 1612
SfaNI	GCATC	3 cut(s)	601, 1509, 1952
SfcI	CTRYAG	2 cut(s)	494, 1947
Sfr274I	CTCGAG	1 cut(s)	606
SfuI	TTCGAA	1 cut(s)	188
SinI	GGWCC	2 cut(s)	140, 1405
SlaI	CTCGAG	1 cut(s)	606
SmiMI	CAYNNNNRTG	1 cut(s)	1400
SmlI	CTYRAG	1 cut(s)	606
SmoI	CTYRAG	1 cut(s)	606
SsiI	CCGC	6 cut(s)	6, 130, 625, 1031, 1063, 1788
SspI	AATATT	1 cut(s)	1414
SspMI	CTAG	2 cut(s)	860, 1521
SstI	GAGCTC	1 cut(s)	765
StyD4I	CCNGG	4 cut(s)	198, 799, 1265, 1716
StyI	CCWWGG	3 cut(s)	557, 1515, 1660
TaaI	ACNGT	4 cut(s)	322, 782, 1875, 1928
TaiI	ACGT	1 cut(s)	952
TaqI	TCGA	6 cut(s)	188, 292, 518, 607, 894, 1329
TaqII	GACCGA	1 cut(s)	1230
TatI	WGTACW	2 cut(s)	856, 1506
TauI	GCSGC	1 cut(s)	1066
Tru1I	TTAA	3 cut(s)	966, 1046, 1221
Tru9I	TTAA	3 cut(s)	966, 1046, 1221
TscAI	CASTG	4 cut(s)	647, 657, 1080, 1965
TseI	GCWGC	8 cut(s)	156, 367, 1172, 1436, 1439, 1599, 1847, 1931
TspDTI	ATGAA	7 cut(s)	276, 806, 921, 1635, 1640, 1737, 1857
TspGWI	ACGGA	3 cut(s)	87, 164, 1701
TspRI	CASTG	4 cut(s)	647, 657, 1080, 1965
VneI	GTGCAC	1 cut(s)	1608
VpaK11BI	GGWCC	2 cut(s)	140, 1405
XapI	RAATTY	4 cut(s)	748, 1118, 1644, 1737
XceI	RCATGY	2 cut(s)	1370, 1639
XhoI	CTCGAG	1 cut(s)	606
XmiI	GTMKAC	1 cut(s)	1099
XmnI	GAANNNNTTC	1 cut(s)	489
XspI	CTAG	2 cut(s)	860, 1521
Zsp2I	ATGCAT	2 cut(s)	616, 1502

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.