pycom16g05380

Glucan endo-1,3-beta-glucosidase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0007655
Physical Location & Seq
Reverse (-)
3494887 .. 3496775
1889 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 585 bp
ATGGCTGCTTTAGTGTATATTGTGATTTTATTGGCCCTCACTGGCCATTCAAGTGCTATTTATTGCGTATGCAAAGATGGAGTTGGTGATTCAGCACTTCAGAAGGCATTGGACTATGCCTGTGGAGCTGGAGCTGACTGCAGTCCAGTCCTCCAAAACGGTGCGTGTTACCAGCCCAACACCATCAAAGATCACTGCAGCTATGCAGTCAACAGTTATTTCCAGAGGAAGGGTCAAACTGCACAGAGCTGTGATTTTTCTGGTGCTGCTTCTCAGAGCCAAACTGCTCCTAGTACCGCAAGTTCGACTTGTGTTTATCCTGCAAGTACCAGCCAAGCAGGAACCGGCTCTACCACTACAAACTCAACCACAGGCACAACTCCAAGCACAGGCACAACTCCAAGCACCAGCACTGGCACCGGCATCACAACTCCAGGCACTCCGGCATCAGTGTTCGGATTAAGCCCCACCGGGAGTACCACAGGAATCAGCAATACCGACGGCGCGGCTACCTTGGGAGGTGCCAACCAGTTCTTTTTCTCTCTAGCCTCAGCTCTGTGCTTCACAGTGTTGTTACTGACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
X8

Protein Analysis

195

Amino Acids

19.3

Weight (kDa)

5.31

Isoelectric Point (pI)

32.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 21 - 91 4.1e-22 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 416, 521
AccII CGCG 1 cut(s) 506
AciI CCGC 2 cut(s) 297, 506
AcoI YGGCCR 1 cut(s) 43
AcuI CTGAAG 1 cut(s) 83
AfaI GTAC 3 cut(s) 295, 328, 478
AfiI CCNNNNNNNGG 2 cut(s) 229, 389
AgsI TTSAA 1 cut(s) 51
AjnI CCWGG 1 cut(s) 433
AluBI AGCT 5 cut(s) 128, 134, 201, 249, 554
AluI AGCT 5 cut(s) 128, 134, 201, 249, 554
AoxI GGCC 2 cut(s) 33, 43
ApeKI GCWGC 3 cut(s) 5, 198, 266
AspLEI GCGC 1 cut(s) 506
AspS9I GGNCC 1 cut(s) 34
AsuC2I CCSGG 1 cut(s) 472
AsuHPI GGTGA 1 cut(s) 98
BalI TGGCCA 1 cut(s) 45
BanI GGYRCC 2 cut(s) 416, 521
BbvCI CCTCAGC 1 cut(s) 550
BbvI GCAGC 2 cut(s) 210, 253
BccI CCATC 2 cut(s) 71, 191
BceAI ACGGC 1 cut(s) 517
BciT130I CCWGG 1 cut(s) 435
BcnI CCSGG 1 cut(s) 472
BfaI CTAG 2 cut(s) 291, 545
BfmI CTRYAG 2 cut(s) 139, 196
BisI GCNGC 4 cut(s) 6, 199, 267, 507
BlsI GCNGC 4 cut(s) 7, 200, 268, 508
Bme1390I CCNGG 2 cut(s) 435, 472
BmgT120I GGNCC 1 cut(s) 34
BmiI GGNNCC 3 cut(s) 343, 418, 523
BmrFI CCNGG 2 cut(s) 435, 472
BmsI GCATC 2 cut(s) 432, 455
BoxI GACNNNNGTC 1 cut(s) 141
BpmI CTGGAG 2 cut(s) 150, 417
Bpu10I CCTNAGC 1 cut(s) 550
BpuMI CCSGG 1 cut(s) 472
BsaJI CCNNGG 1 cut(s) 513
Bsc4I CCNNNNNNNGG 2 cut(s) 229, 389
Bse118I RCCGGY 2 cut(s) 344, 419
Bse1I ACTGG 4 cut(s) 46, 146, 418, 529
BseBI CCWGG 1 cut(s) 435
BseDI CCNNGG 1 cut(s) 513
BseLI CCNNNNNNNGG 2 cut(s) 229, 389
BseMII CTCAG 2 cut(s) 287, 564
BseNI ACTGG 4 cut(s) 46, 146, 418, 529
BseXI GCAGC 2 cut(s) 210, 253
BsgI GTGCAG 1 cut(s) 225
Bsh1236I CGCG 1 cut(s) 506
BshFI GGCC 2 cut(s) 35, 45
BshNI GGYRCC 2 cut(s) 416, 521
BsiSI CCGG 4 cut(s) 345, 420, 443, 471
BslI CCNNNNNNNGG 2 cut(s) 229, 389
BsnI GGCC 2 cut(s) 35, 45
Bsp143I GATC 1 cut(s) 190
BspACI CCGC 2 cut(s) 297, 506
BspANI GGCC 2 cut(s) 35, 45
BspCNI CTCAG 2 cut(s) 286, 563
BspFNI CGCG 1 cut(s) 506
BspLI GGNNCC 3 cut(s) 343, 418, 523
BspMAI CTGCAG 2 cut(s) 143, 200
BspT107I GGYRCC 2 cut(s) 416, 521
BsrFI RCCGGY 2 cut(s) 344, 419
BsrI ACTGG 4 cut(s) 46, 146, 418, 529
BssAI RCCGGY 2 cut(s) 344, 419
BssECI CCNNGG 1 cut(s) 513
BssMI GATC 1 cut(s) 190
BssT1I CCWWGG 1 cut(s) 513
Bst2UI CCWGG 1 cut(s) 435
Bst4CI ACNGT 3 cut(s) 161, 215, 568
BstDEI CTNAG 2 cut(s) 273, 550
BstFNI CGCG 1 cut(s) 506
BstHHI GCGC 1 cut(s) 506
BstKTI GATC 1 cut(s) 193
BstMBI GATC 1 cut(s) 190
BstMWI GCNNNNNNNGC 1 cut(s) 125
BstNI CCWGG 1 cut(s) 435
BstPAI GACNNNNGTC 1 cut(s) 141
BstSCI CCNGG 2 cut(s) 433, 470
BstSFI CTRYAG 2 cut(s) 139, 196
BstUI CGCG 1 cut(s) 506
BstV1I GCAGC 2 cut(s) 210, 253
BsuRI GGCC 2 cut(s) 35, 45
BtsI GCAGTG 1 cut(s) 193
BtsIMutI CAGTG 5 cut(s) 39, 193, 411, 456, 573
CfoI GCGC 1 cut(s) 506
Cfr10I RCCGGY 2 cut(s) 344, 419
Cfr13I GGNCC 1 cut(s) 34
Csp6I GTAC 3 cut(s) 294, 327, 477
CviQI GTAC 3 cut(s) 294, 327, 477
DdeI CTNAG 2 cut(s) 273, 550
DpnI GATC 1 cut(s) 192
DpnII GATC 1 cut(s) 190
EaeI YGGCCR 1 cut(s) 43
Eco130I CCWWGG 1 cut(s) 513
Eco57I CTGAAG 1 cut(s) 83
EcoRII CCWGG 1 cut(s) 433
EcoT14I CCWWGG 1 cut(s) 513
ErhI CCWWGG 1 cut(s) 513
FaiI YATR 4 cut(s) 18, 70, 117, 204
FalI AAGNNNNNCTT 2 cut(s) 292, 324
Fnu4HI GCNGC 4 cut(s) 6, 199, 267, 507
Fsp4HI GCNGC 4 cut(s) 6, 199, 267, 507
FspBI CTAG 2 cut(s) 291, 545
GlaI GCGC 1 cut(s) 505
GluI GCNGC 4 cut(s) 6, 199, 267, 507
GsuI CTGGAG 2 cut(s) 150, 417
HaeIII GGCC 2 cut(s) 35, 45
HapII CCGG 4 cut(s) 345, 420, 443, 471
HhaI GCGC 1 cut(s) 506
Hin6I GCGC 1 cut(s) 504
HinP1I GCGC 1 cut(s) 504
HincII GTYRAC 1 cut(s) 211
HindII GTYRAC 1 cut(s) 211
HinfI GANTC 2 cut(s) 89, 486
HpaII CCGG 4 cut(s) 345, 420, 443, 471
HphI GGTGA 1 cut(s) 98
Hpy166II GTNNAC 1 cut(s) 211
Hpy188I TCNGA 3 cut(s) 102, 276, 458
Hpy188III TCNNGA 1 cut(s) 223
Hpy8I GTNNAC 1 cut(s) 211
Hpy99I CGWCG 1 cut(s) 503
HpyAV CCTTC 2 cut(s) 97, 223
HpyCH4III ACNGT 3 cut(s) 161, 215, 568
HpyCH4V TGCA 6 cut(s) 72, 141, 198, 206, 242, 323
HpyF10VI GCNNNNNNNGC 1 cut(s) 125
HpyF3I CTNAG 2 cut(s) 273, 550
HspAI GCGC 1 cut(s) 504
Kzo9I GATC 1 cut(s) 190
LmnI GCTCC 3 cut(s) 125, 131, 292
Lsp1109I GCAGC 2 cut(s) 210, 253
LweI GCATC 2 cut(s) 432, 455
MaeI CTAG 2 cut(s) 291, 545
MaeIII GTNAC 2 cut(s) 167, 573
MalI GATC 1 cut(s) 192
MboI GATC 1 cut(s) 190
MlsI TGGCCA 1 cut(s) 45
MluNI TGGCCA 1 cut(s) 45
MnlI CCTC 5 cut(s) 47, 161, 219, 512, 559
Mox20I TGGCCA 1 cut(s) 45
MscI TGGCCA 1 cut(s) 45
MseI TTAA 1 cut(s) 461
MslI CAYNNNNRTG 1 cut(s) 51
Msp20I TGGCCA 1 cut(s) 45
MspI CCGG 4 cut(s) 345, 420, 443, 471
MspR9I CCNGG 2 cut(s) 435, 472
MvaI CCWGG 1 cut(s) 435
MvnI CGCG 1 cut(s) 506
MwoI GCNNNNNNNGC 1 cut(s) 125
NciI CCSGG 1 cut(s) 472
NdeII GATC 1 cut(s) 190
NlaIV GGNNCC 3 cut(s) 343, 418, 523
PfeI GAWTC 2 cut(s) 89, 486
PkrI GCNGC 4 cut(s) 7, 200, 268, 508
PshAI GACNNNNGTC 1 cut(s) 141
Psp6I CCWGG 1 cut(s) 433
PspGI CCWGG 1 cut(s) 433
PspN4I GGNNCC 3 cut(s) 343, 418, 523
PspPI GGNCC 1 cut(s) 34
PsrI GAACNNNNNNTAC 4 cut(s) 286, 318, 334, 366
PstI CTGCAG 2 cut(s) 143, 200
RsaI GTAC 3 cut(s) 295, 328, 478
RsaNI GTAC 3 cut(s) 294, 327, 477
RseI CAYNNNNRTG 1 cut(s) 51
SaqAI TTAA 1 cut(s) 461
SatI GCNGC 4 cut(s) 6, 199, 267, 507
Sau3AI GATC 1 cut(s) 190
Sau96I GGNCC 1 cut(s) 34
ScrFI CCNGG 2 cut(s) 435, 472
SetI ASST 8 cut(s) 130, 136, 203, 251, 515, 523, 556, 584
SfaNI GCATC 2 cut(s) 432, 455
SfcI CTRYAG 2 cut(s) 139, 196
SmiMI CAYNNNNRTG 1 cut(s) 51
SsiI CCGC 2 cut(s) 297, 506
SspMI CTAG 2 cut(s) 291, 545
StyD4I CCNGG 2 cut(s) 433, 470
StyI CCWWGG 1 cut(s) 513
TaaI ACNGT 3 cut(s) 161, 215, 568
TaqI TCGA 1 cut(s) 305
TauI GCSGC 1 cut(s) 509
TfiI GAWTC 2 cut(s) 89, 486
Tru1I TTAA 1 cut(s) 461
Tru9I TTAA 1 cut(s) 461
TscAI CASTG 5 cut(s) 46, 200, 418, 456, 573
TseI GCWGC 3 cut(s) 5, 198, 266
TspRI CASTG 5 cut(s) 46, 200, 418, 456, 573
XspI CTAG 2 cut(s) 291, 545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.