pycom16g05740

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0004492
Physical Location & Seq
Forward (+)
3681721 .. 3681939
219 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 219 bp
ATGAGGTACGCGACCCGGGTAAAGGCCATTCTTCTAAGGGGAAAGCTCACAGGGATAGATGGAATGAAGACAAAGGTTCTGGTATGGGTTAAGGTCACTTGTGTAGTGGTTGAAAGTTCCAAGTCCGATAAGGTGTGGTTTACAGCTGGTGTTAAGAAGTCGAGACCAAAGGATGTCTACATAACACCGCGTGACTCCGTGAAAGTAGAAGAATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

73

Amino Acids

8.17

Weight (kDa)

10.07

Isoelectric Point (pI)

15.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 1 - 60 3.1e-12 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 177
AccII CGCG 2 cut(s) 11, 190
AciI CCGC 1 cut(s) 188
AcsI RAATTY 1 cut(s) 212
AdeI CACNNNGTG 1 cut(s) 191
AfaI GTAC 1 cut(s) 8
AfiI CCNNNNNNNGG 1 cut(s) 22
AgsI TTSAA 1 cut(s) 113
AluBI AGCT 2 cut(s) 46, 146
AluI AGCT 2 cut(s) 46, 146
Alw26I GTCTC 1 cut(s) 157
Ama87I CYCGRG 1 cut(s) 15
AoxI GGCC 1 cut(s) 24
ApoI RAATTY 1 cut(s) 212
AsuC2I CCSGG 2 cut(s) 16, 17
AvaI CYCGRG 1 cut(s) 15
BbsI GAAGAC 1 cut(s) 74
BccI CCATC 1 cut(s) 53
BcnI CCSGG 2 cut(s) 16, 17
BcoDI GTCTC 1 cut(s) 157
Bme1390I CCNGG 2 cut(s) 16, 17
BmeT110I CYCGRG 1 cut(s) 15
BmrFI CCNGG 2 cut(s) 16, 17
BpiI GAAGAC 1 cut(s) 74
BpuMI CCSGG 2 cut(s) 16, 17
BsaI GGTCTC 1 cut(s) 157
BsaJI CCNNGG 1 cut(s) 15
Bsc4I CCNNNNNNNGG 1 cut(s) 22
BseDI CCNNGG 1 cut(s) 15
BseGI GGATG 1 cut(s) 178
BseLI CCNNNNNNNGG 1 cut(s) 22
Bsh1236I CGCG 2 cut(s) 11, 190
BshFI GGCC 1 cut(s) 26
BsiHKCI CYCGRG 1 cut(s) 15
BsiSI CCGG 1 cut(s) 16
BslI CCNNNNNNNGG 1 cut(s) 22
BsmAI GTCTC 1 cut(s) 157
BsnI GGCC 1 cut(s) 26
Bso31I GGTCTC 1 cut(s) 157
BsoBI CYCGRG 1 cut(s) 15
BspACI CCGC 1 cut(s) 188
BspANI GGCC 1 cut(s) 26
BspFNI CGCG 2 cut(s) 11, 190
BspTNI GGTCTC 1 cut(s) 157
BssECI CCNNGG 1 cut(s) 15
BstDEI CTNAG 1 cut(s) 35
BstF5I GGATG 1 cut(s) 178
BstFNI CGCG 2 cut(s) 11, 190
BstMAI GTCTC 1 cut(s) 157
BstSCI CCNGG 2 cut(s) 14, 15
BstUI CGCG 2 cut(s) 11, 190
BstV2I GAAGAC 1 cut(s) 74
BsuRI GGCC 1 cut(s) 26
BtsCI GGATG 1 cut(s) 178
Cfr9I CCCGGG 1 cut(s) 15
Csp6I GTAC 1 cut(s) 7
CviJI RGCY 3 cut(s) 26, 46, 146
CviKI_1 RGCY 3 cut(s) 26, 46, 146
CviQI GTAC 1 cut(s) 7
DdeI CTNAG 1 cut(s) 35
DraIII CACNNNGTG 1 cut(s) 191
Eco31I GGTCTC 1 cut(s) 157
Eco88I CYCGRG 1 cut(s) 15
EcoRI GAATTC 1 cut(s) 212
FaiI YATR 2 cut(s) 85, 182
FblI GTMKAC 1 cut(s) 177
FokI GGATG 1 cut(s) 185
HaeIII GGCC 1 cut(s) 26
HapII CCGG 1 cut(s) 16
HinfI GANTC 1 cut(s) 194
HpaII CCGG 1 cut(s) 16
Hpy166II GTNNAC 2 cut(s) 141, 178
Hpy188I TCNGA 2 cut(s) 127, 218
Hpy188III TCNNGA 1 cut(s) 162
Hpy8I GTNNAC 2 cut(s) 141, 178
HpyF3I CTNAG 1 cut(s) 35
LpnPI CCDG 4 cut(s) 29, 36, 65, 132
MaeIII GTNAC 2 cut(s) 94, 191
MboII GAAGA 2 cut(s) 23, 79
MluCI AATT 1 cut(s) 212
MlyI GAGTC 1 cut(s) 188
MseI TTAA 2 cut(s) 90, 153
MspA1I CMGCKG 1 cut(s) 146
MspI CCGG 1 cut(s) 16
MspR9I CCNGG 2 cut(s) 16, 17
MvnI CGCG 2 cut(s) 11, 190
NciI CCSGG 2 cut(s) 16, 17
NmuCI GTSAC 2 cut(s) 94, 191
PleI GAGTC 1 cut(s) 188
PpsI GAGTC 1 cut(s) 188
PvuII CAGCTG 1 cut(s) 146
RsaI GTAC 1 cut(s) 8
RsaNI GTAC 1 cut(s) 7
SaqAI TTAA 2 cut(s) 90, 153
SchI GAGTC 1 cut(s) 188
ScrFI CCNGG 2 cut(s) 16, 17
SetI ASST 6 cut(s) 8, 48, 78, 96, 135, 148
SmaI CCCGGG 1 cut(s) 17
Sse9I AATT 1 cut(s) 212
SsiI CCGC 1 cut(s) 188
StyD4I CCNGG 2 cut(s) 14, 15
TaqI TCGA 1 cut(s) 161
TasI AATT 1 cut(s) 212
Tru1I TTAA 2 cut(s) 90, 153
Tru9I TTAA 2 cut(s) 90, 153
TseFI GTSAC 2 cut(s) 94, 191
Tsp45I GTSAC 2 cut(s) 94, 191
TspDTI ATGAA 1 cut(s) 80
TspGWI ACGGA 1 cut(s) 187
TspMI CCCGGG 1 cut(s) 15
XapI RAATTY 1 cut(s) 212
XmaI CCCGGG 1 cut(s) 15
XmiI GTMKAC 1 cut(s) 177
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.