pycom16g05750

Uncharacterised ACR, YagE family COG1723

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0005702
Physical Location & Seq
Reverse (-)
3682385 .. 3685218
2834 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1101 bp
ATGGGGAGGTGGAGAGCCGCTGCTTCTCTTCTCCTCAGCCCCGTAGCCGCCGCCGGAGCTTCTAAATCTTCTGTTCCCGCCAAGCCTCCTTTCCATCTCCACCGCCCTTCAGCCTCCACCTCCTTCTACTTCCGCTTCCTCAATTCACGACCTTTCTCTGCAATCCCGTCTCGGGTTTCCGTTGAAGCCAATGATTACGACTCTGAGCCGCCCTTCTATGCTCAGAGTCAGAGCCAAGCTAAGGAAGACGAGGAAACCGGAAAAATACCCGTTAAAGCCTACTTCCTTTGTACCAGTATCAATTTGAAGAGCATGCAAGCCGAGAATTTAAGCAATGTCATTCCTCCTACTTCTCGCTCATCGAATTACATTGCCCTCAGATTTTGCGATTTCCCTTCAGAAAATACTGAATTTGGAGTCTGGCGTTACATGGTCGTATTCCAGTATGGATCTGCTGTTCTGTTTAATGTTGAGGATCATGAAGTTGACGGTTATATCGATCTAGTTACAAGACATGCTTCTGGATTACTTCCAGAGATGAGAAAAGACGATTATGCCGTAAAAGAAAAGCCACAGCTGGATGAGGACATGCAGGGTGGTCCAGACTACATAGTTCTCAAAACTTTGGACACTGATTCTATTCGCATTATTGGCAGTGTTCTTGGCCAAAGTATTGCCTTAGATTATTTTGTTTCGCAGGTTGATGGTATGGTTGAAGAGTTTGCAGATATAAATCGTGGAATGGAGAAAACTGGAACTTTCACAATGCATAAAAAGAAGCTCCTTCAACTTGTTGGGAAGGCTAACTCGAACTTAGCTGATGTAATTCTTAAAGTAGGTCTTTTTGAGAGATCAGAAATTGCTTGGAGGGATGCAAAGTATGCTCAAATACATGAGTACCTTCGGGAGGAGTACGAGGTTACACAACGCTTCGGAAACTTGGATTTCAAGTTAAAGTTTGTAGAGCACAACATTCATTTCTTGCAAGAAGTCCTTCAAAACAGAAAGTCAGATCTTCTGGAATGGTGCATCATCTTCCTGTTGAGCATAGAAAACATTATATCGCTGTACGAGATTGTTCGTGATTCATCTGCAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF155

Protein Analysis

367

Amino Acids

41.5

Weight (kDa)

5.4

Isoelectric Point (pI)

50.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF155 PF02582 144 - 314 1.5e-32 RMND1/Sif2-Sif3/Mrx10, DUF155
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 688
AciI CCGC 7 cut(s) 18, 48, 51, 78, 103, 133, 209
AclWI GGATC 2 cut(s) 457, 483
AcoI YGGCCR 1 cut(s) 664
AcsI RAATTY 2 cut(s) 325, 410
AcuI CTGAAG 2 cut(s) 93, 381
AfaI GTAC 4 cut(s) 292, 899, 914, 1070
AfiI CCNNNNNNNGG 3 cut(s) 172, 241, 907
AgsI TTSAA 6 cut(s) 185, 307, 716, 788, 949, 998
AjuI GAANNNNNNNTTGG 2 cut(s) 74, 106
AloI GAACNNNNNNTCC 2 cut(s) 441, 473
AluBI AGCT 6 cut(s) 59, 239, 577, 781, 818, 1097
AluI AGCT 6 cut(s) 59, 239, 577, 781, 818, 1097
Alw21I GWGCWC 1 cut(s) 969
Alw26I GTCTC 1 cut(s) 174
AlwI GGATC 2 cut(s) 457, 483
Ama87I CYCGRG 1 cut(s) 171
AoxI GGCC 1 cut(s) 664
ApeKI GCWGC 2 cut(s) 20, 1094
ApoI RAATTY 2 cut(s) 325, 410
Asp700I GAANNNNTTC 1 cut(s) 993
AspS9I GGNCC 1 cut(s) 599
AvaI CYCGRG 1 cut(s) 171
AvaII GGWCC 1 cut(s) 599
BaeI ACNNNNGTAYC 2 cut(s) 881, 914
BalI TGGCCA 1 cut(s) 666
BbsI GAAGAC 1 cut(s) 252
Bbv12I GWGCWC 1 cut(s) 969
BbvCI CCTCAGC 1 cut(s) 35
BbvI GCAGC 1 cut(s) 7
BccI CCATC 2 cut(s) 102, 698
BceAI ACGGC 1 cut(s) 542
BcoDI GTCTC 1 cut(s) 174
BfaI CTAG 1 cut(s) 503
BfmI CTRYAG 1 cut(s) 1092
BfuAI ACCTGC 1 cut(s) 688
BglII AGATCT 1 cut(s) 1012
BisI GCNGC 6 cut(s) 18, 21, 48, 51, 209, 1095
BlsI GCNGC 6 cut(s) 19, 22, 49, 52, 210, 1096
Bme18I GGWCC 1 cut(s) 599
BmeT110I CYCGRG 1 cut(s) 171
BmgT120I GGNCC 1 cut(s) 599
BmsI GCATC 2 cut(s) 862, 1038
BpiI GAAGAC 1 cut(s) 252
Bpu10I CCTNAGC 2 cut(s) 35, 240
Bsa29I ATCGAT 1 cut(s) 498
BsaBI GATNNNNATC 1 cut(s) 732
BsaWI WCCGGW 1 cut(s) 257
Bsc4I CCNNNNNNNGG 3 cut(s) 172, 241, 907
Bse1I ACTGG 3 cut(s) 294, 442, 757
Bse3DI GCAATG 2 cut(s) 340, 369
Bse8I GATNNNNATC 1 cut(s) 732
BseCI ATCGAT 1 cut(s) 498
BseGI GGATG 2 cut(s) 586, 877
BseJI GATNNNNATC 1 cut(s) 732
BseLI CCNNNNNNNGG 3 cut(s) 172, 241, 907
BseMI GCAATG 2 cut(s) 340, 369
BseMII CTCAG 4 cut(s) 49, 195, 236, 391
BseNI ACTGG 3 cut(s) 294, 442, 757
BseRI GAGGAG 2 cut(s) 23, 923
BseXI GCAGC 1 cut(s) 7
BshFI GGCC 1 cut(s) 666
BshVI ATCGAT 1 cut(s) 498
BsiHKAI GWGCWC 1 cut(s) 969
BsiHKCI CYCGRG 1 cut(s) 171
BsiSI CCGG 2 cut(s) 54, 258
BslI CCNNNNNNNGG 3 cut(s) 172, 241, 907
BsmAI GTCTC 1 cut(s) 174
BsmBI CGTCTC 1 cut(s) 174
BsnI GGCC 1 cut(s) 666
BsoBI CYCGRG 1 cut(s) 171
Bsp1286I GDGCHC 1 cut(s) 969
Bsp143I GATC 5 cut(s) 449, 475, 499, 851, 1012
BspACI CCGC 7 cut(s) 18, 48, 51, 78, 103, 133, 209
BspANI GGCC 1 cut(s) 666
BspCNI CTCAG 4 cut(s) 48, 196, 235, 390
BspDI ATCGAT 1 cut(s) 498
BspHI TCATGA 1 cut(s) 478
BspMAI CTGCAG 1 cut(s) 1096
BspMI ACCTGC 1 cut(s) 688
BspPI GGATC 2 cut(s) 457, 483
BspQI GCTCTTC 1 cut(s) 302
BsrDI GCAATG 2 cut(s) 340, 369
BsrI ACTGG 3 cut(s) 294, 442, 757
BssMI GATC 5 cut(s) 449, 475, 499, 851, 1012
Bst4CI ACNGT 1 cut(s) 491
Bst6I CTCTTC 3 cut(s) 33, 302, 711
BstAPI GCANNNNNTGC 1 cut(s) 881
BstC8I GCNNGC 2 cut(s) 314, 318
BstDEI CTNAG 7 cut(s) 35, 204, 222, 240, 377, 679, 814
BstENI CCTNNNNNAGG 1 cut(s) 905
BstF5I GGATG 2 cut(s) 586, 877
BstKTI GATC 5 cut(s) 452, 478, 502, 854, 1015
BstMAI GTCTC 1 cut(s) 174
BstMBI GATC 5 cut(s) 449, 475, 499, 851, 1012
BstMWI GCNNNNNNNGC 3 cut(s) 56, 651, 881
BstNSI RCATGY 3 cut(s) 316, 518, 592
BstSFI CTRYAG 1 cut(s) 1092
BstV1I GCAGC 1 cut(s) 7
BstV2I GAAGAC 1 cut(s) 252
BstX2I RGATCY 2 cut(s) 449, 1012
BstYI RGATCY 2 cut(s) 449, 1012
Bsu15I ATCGAT 1 cut(s) 498
BsuRI GGCC 1 cut(s) 666
BsuTUI ATCGAT 1 cut(s) 498
BtsCI GGATG 2 cut(s) 586, 877
BtsI GCAGTG 1 cut(s) 661
BtsIMutI CAGTG 2 cut(s) 630, 661
BveI ACCTGC 1 cut(s) 688
Cac8I GCNNGC 2 cut(s) 314, 318
CciI TCATGA 1 cut(s) 478
Cfr13I GGNCC 1 cut(s) 599
ClaI ATCGAT 1 cut(s) 498
Csp6I GTAC 4 cut(s) 291, 898, 913, 1069
CviAII CATG 6 cut(s) 313, 430, 479, 515, 589, 893
CviQI GTAC 4 cut(s) 291, 898, 913, 1069
DdeI CTNAG 7 cut(s) 35, 204, 222, 240, 377, 679, 814
DpnI GATC 5 cut(s) 451, 477, 501, 853, 1014
DpnII GATC 5 cut(s) 449, 475, 499, 851, 1012
EaeI YGGCCR 1 cut(s) 664
Eam1104I CTCTTC 3 cut(s) 33, 302, 711
EarI CTCTTC 3 cut(s) 33, 302, 711
Eco47I GGWCC 1 cut(s) 599
Eco57I CTGAAG 2 cut(s) 93, 381
Eco88I CYCGRG 1 cut(s) 171
EcoNI CCTNNNNNAGG 1 cut(s) 905
EcoT22I ATGCAT 1 cut(s) 771
Esp3I CGTCTC 1 cut(s) 174
FaeI CATG 6 cut(s) 316, 433, 482, 518, 592, 896
FalI AAGNNNNNCTT 6 cut(s) 502, 534, 825, 857, 978, 1010
FatI CATG 6 cut(s) 312, 429, 478, 514, 588, 892
FauI CCCGC 1 cut(s) 85
Fnu4HI GCNGC 6 cut(s) 18, 21, 48, 51, 209, 1095
FokI GGATG 2 cut(s) 593, 884
Fsp4HI GCNGC 6 cut(s) 18, 21, 48, 51, 209, 1095
FspBI CTAG 1 cut(s) 503
GluI GCNGC 6 cut(s) 18, 21, 48, 51, 209, 1095
HaeIII GGCC 1 cut(s) 666
HapII CCGG 2 cut(s) 54, 258
Hin1II CATG 6 cut(s) 316, 433, 482, 518, 592, 896
HincII GTYRAC 1 cut(s) 487
HindII GTYRAC 1 cut(s) 487
HinfI GANTC 5 cut(s) 200, 226, 417, 635, 1085
HpaII CCGG 2 cut(s) 54, 258
Hpy166II GTNNAC 1 cut(s) 487
Hpy188I TCNGA 8 cut(s) 205, 225, 231, 380, 400, 856, 935, 1012
Hpy188III TCNNGA 8 cut(s) 147, 479, 522, 533, 602, 905, 1019, 1082
Hpy8I GTNNAC 1 cut(s) 487
HpyAV CCTTC 8 cut(s) 117, 133, 223, 405, 793, 794, 911, 1004
HpyCH4III ACNGT 1 cut(s) 491
HpyCH4V TGCA 9 cut(s) 161, 316, 592, 725, 769, 875, 985, 1029, 1094
HpyF10VI GCNNNNNNNGC 3 cut(s) 56, 651, 881
HpyF3I CTNAG 7 cut(s) 35, 204, 222, 240, 377, 679, 814
Hsp92II CATG 6 cut(s) 316, 433, 482, 518, 592, 896
Kzo9I GATC 5 cut(s) 449, 475, 499, 851, 1012
LguI GCTCTTC 1 cut(s) 302
LmnI GCTCC 2 cut(s) 56, 786
Lsp1109I GCAGC 1 cut(s) 7
LweI GCATC 2 cut(s) 862, 1038
MaeI CTAG 1 cut(s) 503
MaeIII GTNAC 3 cut(s) 425, 505, 919
MalI GATC 5 cut(s) 451, 477, 501, 853, 1014
MboI GATC 5 cut(s) 449, 475, 499, 851, 1012
MboII GAAGA 7 cut(s) 20, 60, 257, 319, 728, 1007, 1027
MflI RGATCY 2 cut(s) 449, 1012
MhlI GDGCHC 1 cut(s) 969
MlsI TGGCCA 1 cut(s) 666
MluCI AATT 7 cut(s) 142, 301, 325, 364, 410, 825, 858
MluNI TGGCCA 1 cut(s) 666
MlyI GAGTC 3 cut(s) 194, 235, 426
Mox20I TGGCCA 1 cut(s) 666
Mph1103I ATGCAT 1 cut(s) 771
MroXI GAANNNNTTC 1 cut(s) 993
MscI TGGCCA 1 cut(s) 666
MseI TTAA 6 cut(s) 273, 329, 465, 831, 953, 1099
Msp20I TGGCCA 1 cut(s) 666
MspA1I CMGCKG 2 cut(s) 20, 577
MspI CCGG 2 cut(s) 54, 258
MwoI GCNNNNNNNGC 3 cut(s) 56, 651, 881
NdeII GATC 5 cut(s) 449, 475, 499, 851, 1012
NlaIII CATG 6 cut(s) 316, 433, 482, 518, 592, 896
NmeAIII GCCGAG 1 cut(s) 346
NsiI ATGCAT 1 cut(s) 771
NspI RCATGY 3 cut(s) 316, 518, 592
PaeI GCATGC 1 cut(s) 316
PagI TCATGA 1 cut(s) 478
PciSI GCTCTTC 1 cut(s) 302
PcsI WCGNNNNNNNCGW 2 cut(s) 495, 555
PdmI GAANNNNTTC 1 cut(s) 993
PfeI GAWTC 2 cut(s) 635, 1085
PkrI GCNGC 6 cut(s) 19, 22, 49, 52, 210, 1096
PleI GAGTC 3 cut(s) 194, 234, 425
PpsI GAGTC 3 cut(s) 194, 234, 425
PspPI GGNCC 1 cut(s) 599
PstI CTGCAG 1 cut(s) 1096
PsuI RGATCY 2 cut(s) 449, 1012
PvuII CAGCTG 1 cut(s) 577
RsaI GTAC 4 cut(s) 292, 899, 914, 1070
RsaNI GTAC 4 cut(s) 291, 898, 913, 1069
SapI GCTCTTC 1 cut(s) 302
SaqAI TTAA 6 cut(s) 273, 329, 465, 831, 953, 1099
SatI GCNGC 6 cut(s) 18, 21, 48, 51, 209, 1095
Sau3AI GATC 5 cut(s) 449, 475, 499, 851, 1012
Sau96I GGNCC 1 cut(s) 599
SchI GAGTC 3 cut(s) 194, 235, 426
SduI GDGCHC 1 cut(s) 969
SfaNI GCATC 2 cut(s) 862, 1038
SfcI CTRYAG 1 cut(s) 1092
SinI GGWCC 1 cut(s) 599
SphI GCATGC 1 cut(s) 316
Sse9I AATT 7 cut(s) 142, 301, 325, 364, 410, 825, 858
SsiI CCGC 7 cut(s) 18, 48, 51, 78, 103, 133, 209
SspMI CTAG 1 cut(s) 503
TaaI ACNGT 1 cut(s) 491
TaqI TCGA 3 cut(s) 362, 498, 809
TasI AATT 7 cut(s) 142, 301, 325, 364, 410, 825, 858
TauI GCSGC 4 cut(s) 20, 50, 53, 211
TfiI GAWTC 2 cut(s) 635, 1085
Tru1I TTAA 6 cut(s) 273, 329, 465, 831, 953, 1099
Tru9I TTAA 6 cut(s) 273, 329, 465, 831, 953, 1099
TscAI CASTG 2 cut(s) 637, 661
TseI GCWGC 2 cut(s) 20, 1094
TspDTI ATGAA 3 cut(s) 495, 965, 1077
TspGWI ACGGA 1 cut(s) 169
TspRI CASTG 2 cut(s) 637, 661
VpaK11BI GGWCC 1 cut(s) 599
XagI CCTNNNNNAGG 1 cut(s) 905
XapI RAATTY 2 cut(s) 325, 410
XceI RCATGY 3 cut(s) 316, 518, 592
XmnI GAANNNNTTC 1 cut(s) 993
XspI CTAG 1 cut(s) 503
Zsp2I ATGCAT 1 cut(s) 771
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.