Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0001202

Physical Location & Seq

Genomic Coordinates Reverse (-)

5048833 .. 5051615

Gene Structure 2783 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2586 bp

ATGGCGTCAAGATCGATCTGCGCAAACCTTTTTGATTTGGCATCTGGAGGCCTTTTGGATATTCCTTGTACTCCAAGGGCTCTTCCAAGGGTGATGACTGTTCCTGGTATTATATCTGATGTGGATAGTAATAGCAATGGTGACGATTCAGATGCCAGTTCATCTGCCTGTCGTGAGAGGAAAATTGTTGTGGCAAATATGTTACCATTACATGCTAAAAGGGATCCACAAACGAAGAAATGGTGTTTCAGTTTGGATGAGGATTCAATTTTGTTACATTTGAAGGATGGGTTTTCATCTGAAAATGAGGTTGTTTATGTGGGGTCTCTCAAGGCTGAAATTGATACCAGTGAACAAGAAGAAGTCGCACAGAAACTGCTGGAGGAGTTCAATTGTGTACCAACGTTCTTGCCCAGTGATCTCCAGAAGAAGTTTTATTTGGGGTTCTGCAAGCAGCAATTATGGCCTCTTTTTCATTACATGCTGCCCATGTGTCCAGACCACGGCGACCGATTTGATCGCTCACTGTGGCGGGCCTATGTATCTGCAAACAAAATATTTGCAGACAAGGTGATGGAAGTTATTAATCCCGAGGACGATTGTATCTGGGTTCATGACTATCACTTGATGGTTCTCCCAACGTTTTTGAGGAAGCGGTACTATCGAGTAAAGCTAGGATTCTTCCTTCACAGCCCATTTCCCTCATCCGAAATTTATCGAACTTTGCCGGTCCGTGATGAAATTTTGAGGGGTTTGCTAAACTGCGATCTCATTGGGTTTCATACATTTGATTATGCACGTCACTTCCTCTCATGCTGCAGTAGAATGCTGGGGCTGGACTATGAATCAAAGCGAGGACATATTGGGCTTGATTATTTTGGCCGCACAGTGTACATTAAAATTCTGCCTGTAGGTGTTCATATGGGTCGGCTTGAATCTGTGTTGAATCTTCCCGATACAACTTCCAAAATTAAAGAGATTCAAGAGCAGTATAAGGGGAAAAAATTGATTCTTGGTATTGATGACATGGATATATTTAAAGGAATCAGCTTAAAGTTTATAGCTTTGGAACAACTCTTGCAGCAGAATCCAGAATTGCAGGGCAACATAGTTCTGGTTCAAATTATAAATCCCGCAAGGGGATCGGGGAAAGATGTACAGGAAGCAAAGAGTGAGACATACTTAACTGCCAGAAGGATAAATGAGGTTTATGGTTCACCTAATTATGAACCAGTGGTCCTGATTGATCGACATGTTCCTCAATACGAGAAGACAGCGTATTATGCTGTAGCAGAATGTTGTATAGTGAATGCTGTGAGGGATGGAATGAACTTAGTTCCTTACAAATATATTGTTTGCCGGCAGGGAACTCCACCCATGAATACAGCTTTAGGCATGACAGCAGATTCTCCTCAGACAAGCATGCTTGTTGTGTCTGAATTTATTGGCTGCTCACCTTCTTTAAGTGGAGCAATCAGGGTTAACCCTTGGGACACTGATGCCGTGGCTGATGCATTGAACTTGGCGATCACCATGTCTAAATCAGAGCAGCAGTTACGTCATGAAAAACACTATCGTTATGTTAGTTCTCATGATGTGGCTTATTGGGCACGAAGCTTTGCACAGGACTTGGATAGAGCATGCCGAGATCATTATAGTAAACGGTGTTGGGGCATTGGATTGGGCTTGAGGTTTAGAGTTGTTTCCCTCTCTCCAAACTTTAGAAAGTTGTCTATAGACCATATTGTTTCAGCATACCAAAGAACAAATAGAAGGGCCATATTTCTGGACTATGATGGTACCATTATTCCTGAAGCTTCCATTATTAAGTCTCCAAGCCCAGAAGTCCTTGCTCTTATGAATTCCCTGTGCAAGGATCCAAAGAACACAGTTTTCATTGTTAGTGGGAGGGGAAGGACTTCTCTGAGTGATTGGTTTGCCTCGTGTGAGACGCTTGGAATAGCTGCTGAACATGGGTACTTTCTAAGGTGGAATAGAAGTTCCGAGTGGGAGACCAGTCCTGTTGGTGCCGATCTTGATTGGAAAGAAATTGTGGAGCCTGTGATGAGATTGTATACAGAGGCAACGGATGGCTCCAACATAGAAAGTAAAGAAAGCGCTTTGGTATGGCATCACCAAGATGCAGACCCCGACTTTGGATCCTGTCAAGCCAAGGAATTGTTGGATCATCTGGAAAATGTTCTTTCGAATGAACCAGCAGTTGTTAAGAGGGGCCAGCATATAGTTGAAGTTAAGCCACAGGGAGTAAGCAAAGGAGTGGTTGCTGAAAAAATTCTTTCGAGAATGGCCAACGATGGGAAGGCCCCCAACTTTGTGATGTGCATTGGGGATGACAGATCCGACGAGGACATGTTCGAGAGCATATTAAGCACGGTCTCTAGTCCATCTTTGCCTTCACCACCCGAGATATTTGCCTGCACCGTCGGAAGAAAGCAAAGCAAGGCCAAGTACTATCTCGATGATGTCAGTGATGTTGTGAAGTTACTTCAAGGCCTTGCAACTGCTTCGACCCCGAAGCCGAAGCATCTCCCCCATATCCAGGTCTCTTTCGAAAGTGTCTATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005984 GO:0005991 GO:0005992 GO:0006793 GO:0006796 GO:0006950 GO:0008150 GO:0008152 GO:0009058 GO:0009311 GO:0009312 GO:0009987 GO:0016051 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0033554 GO:0034637 GO:0042578 GO:0044237 GO:0044238 GO:0044249 GO:0044262 GO:0044424 GO:0044464 GO:0046351 GO:0050896 GO:0051716 GO:0070413 GO:0071704 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

Glyco_transf_20 Trehalose_PPase

Protein Analysis

862

Amino Acids

96.97

Weight (kDa)

6.03

Isoelectric Point (pI)

51.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_transf_20	PF00982	60 - 545	9.9e-191	Glycosyltransferase family 20
Trehalose_PPase	PF02358	595 - 830	2.2e-84	Trehalose-phosphatase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001202)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G23870 AT1G60140 AT1G60140 AT1G60140 AT1G60140 AT1G60140 AT1G60140 AT1G70290 AT1G70290
fragaria_vesca	FvH4_4g34800 FvH4_4g34800 FvH4_4g34800 FvH4_4g34800 FvH4_4g34800 FvH4_4g34800 FvH4_4g34800 FvH4_5g05100 FvH4_5g05100 FvH4_5g05100 FvH4_5g05100 FvH4_5g05100 FvH4_5g05100 FvH4_5g05100 FvH4_5g05100 FvH4_5g05100
malus_domestica	MD00G1171800.v1.1 MD13G1089600.v1.1 MD14G1183700.v1.1 MD16G1089800.v1.1
prunus_persica	Prupe.1G256200_v2.0.a1 Prupe.5G176400_v2.0.a1
pyrus_communis	pycom06g15770 pycom13g07810 pycom14g15280 pycom16g07690
rosa_chinensis	RchiOBHm_Chr4g0444141 RchiOBHm_Chr7g0193901
rosa_laevigata	RLG00000004229 RLG00000004235 RLG00000005852
rosa_multiflora	Rmu_co8448227.1_g000001 Rmu_sc0012638.1_g000011
rosa_roxburghii	Rroxscaffold_3G00261720 Rroxscaffold_5G00384730
rosa_rugosa	Rorug04G0350400 Rorug07G0012500 Rorug07G0012600 Rorug07G0012700
rosa_samantha	Rh4AG411000 Rh4BG422400 Rh4CG436800 Rh4DG417700 Rh7BG140000 Rh7CG143700
rosa_wichuraiana	Rw4G035310 Rw7G011900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1127
Acc16I	TGCGCA	1 cut(s)	22
Acc65I	GGTACC	1 cut(s)	1799
AccB1I	GGYRCC	2 cut(s)	1799, 2027
AccI	GTMKAC	1 cut(s)	2075
AciI	CCGC	4 cut(s)	532, 655, 883, 1134
AclI	AACGTT	2 cut(s)	404, 641
AclWI	GGATC	9 cut(s)	218, 231, 1150, 1871, 1884, 2154, 2167, 2193, 2352
AcoI	YGGCCR	2 cut(s)	880, 2307
AcsI	RAATTY	6 cut(s)	711, 741, 900, 1439, 1861, 2292
AcuI	CTGAAG	1 cut(s)	1833
AcyI	GRCGYC	1 cut(s)	5
AfaI	GTAC	8 cut(s)	70, 399, 659, 893, 1158, 1801, 1979, 2471
AfeI	AGCGCT	1 cut(s)	2119
AfiI	CCNNNNNNNGG	6 cut(s)	503, 1139, 1873, 2156, 2316, 2560
AflIII	ACRYGT	2 cut(s)	1252, 2370
AjiI	CACGTC	1 cut(s)	800
AjnI	CCWGG	2 cut(s)	103, 2559
AjuI	GAANNNNNNNTTGG	2 cut(s)	422, 454
AloI	GAACNNNNNNTCC	4 cut(s)	1221, 1253, 1984, 2016
AluBI	AGCT	7 cut(s)	673, 1050, 1064, 1388, 1617, 1817, 1964
AluI	AGCT	7 cut(s)	673, 1050, 1064, 1388, 1617, 1817, 1964
Alw26I	GTCTC	7 cut(s)	330, 1169, 1836, 1943, 2006, 2401, 2569
AlwI	GGATC	9 cut(s)	218, 231, 1150, 1871, 1884, 2154, 2167, 2193, 2352
AlwNI	CAGNNNCTG	1 cut(s)	376
Ama87I	CYCGRG	2 cut(s)	590, 2423
Aor51HI	AGCGCT	1 cut(s)	2119
ApeKI	GCWGC	7 cut(s)	454, 484, 816, 1081, 1449, 1549, 1964
ApoI	RAATTY	6 cut(s)	711, 741, 900, 1439, 1861, 2292
ArsI	GACNNNNNNTTYG	2 cut(s)	2138, 2170
AseI	ATTAAT	1 cut(s)	585
Asp700I	GAANNNNTTC	3 cut(s)	1918, 2199, 2292
Asp718I	GGTACC	1 cut(s)	1799
AspLEI	GCGC	2 cut(s)	23, 2120
AspS9I	GGNCC	6 cut(s)	534, 730, 1237, 1776, 2233, 2323
AsuHPI	GGTGA	8 cut(s)	103, 152, 583, 1209, 1446, 1522, 2126, 2409
AsuII	TTCGAA	2 cut(s)	2207, 2571
AvaI	CYCGRG	2 cut(s)	590, 2423
AvaII	GGWCC	2 cut(s)	730, 1237
BaeGI	GKGCMC	1 cut(s)	1612
BaeI	ACNNNNGTAYC	2 cut(s)	586, 619
BalI	TGGCCA	1 cut(s)	2309
BamHI	GGATCC	3 cut(s)	223, 1876, 2159
BanI	GGYRCC	2 cut(s)	1799, 2027
BanII	GRGCYC	1 cut(s)	82
BauI	CACGAG	1 cut(s)	1942
BbsI	GAAGAC	1 cut(s)	1277
BbvI	GCAGC	7 cut(s)	466, 471, 803, 1093, 1436, 1561, 1951
BccI	CCATC	8 cut(s)	281, 568, 622, 1316, 1790, 2084, 2309, 2413
BceAI	ACGGC	2 cut(s)	520, 1487
BcgI	CGANNNNNNTGC	8 cut(s)	134, 168, 1125, 1159, 2414, 2448, 2508, 2542
BciT130I	CCWGG	2 cut(s)	105, 2561
BcoDI	GTCTC	7 cut(s)	330, 1169, 1836, 1943, 2006, 2401, 2569
BfaI	CTAG	2 cut(s)	674, 2400
BfmI	CTRYAG	4 cut(s)	817, 909, 1287, 1734
BfoI	RGCGCY	1 cut(s)	2121
BisI	GCNGC	8 cut(s)	455, 485, 817, 883, 1082, 1450, 1550, 1965
BlsI	GCNGC	8 cut(s)	456, 486, 818, 884, 1083, 1451, 1551, 1966
BmcAI	AGTACT	1 cut(s)	2471
Bme1390I	CCNGG	2 cut(s)	105, 2561
Bme18I	GGWCC	2 cut(s)	730, 1237
BmeT110I	CYCGRG	2 cut(s)	590, 2423
BmgBI	CACGTC	1 cut(s)	800
BmgT120I	GGNCC	6 cut(s)	534, 730, 1237, 1776, 2233, 2323
BmiI	GGNNCC	9 cut(s)	225, 1801, 1878, 2029, 2058, 2095, 2161, 2234, 2325
BmrFI	CCNGG	2 cut(s)	105, 2561
BmrI	ACTGGG	1 cut(s)	408
BmsI	GCATC	7 cut(s)	50, 142, 1489, 1501, 2131, 2140, 2554
BmuI	ACTGGG	1 cut(s)	408
BpiI	GAAGAC	1 cut(s)	1277
BpmI	CTGGAG	3 cut(s)	66, 401, 407
Bpu14I	TTCGAA	2 cut(s)	2207, 2571
BpuEI	CTTGAG	2 cut(s)	314, 1708
Bsa29I	ATCGAT	1 cut(s)	14
BsaHI	GRCGYC	1 cut(s)	5
BsaI	GGTCTC	4 cut(s)	330, 2006, 2401, 2569
BsaJI	CCNNGG	7 cut(s)	74, 86, 502, 591, 1487, 1503, 2172
Bsc4I	CCNNNNNNNGG	6 cut(s)	503, 1139, 1873, 2156, 2316, 2560
Bse118I	RCCGGY	2 cut(s)	727, 1359
Bse1I	ACTGG	5 cut(s)	156, 348, 414, 1232, 2016
Bse3DI	GCAATG	1 cut(s)	142
BseBI	CCWGG	2 cut(s)	105, 2561
BseCI	ATCGAT	1 cut(s)	14
BseDI	CCNNGG	7 cut(s)	74, 86, 502, 591, 1487, 1503, 2172
BseGI	GGATG	6 cut(s)	262, 292, 704, 1327, 2095, 2356
BseLI	CCNNNNNNNGG	6 cut(s)	503, 1139, 1873, 2156, 2316, 2560
BseMI	GCAATG	1 cut(s)	142
BseMII	CTCAG	2 cut(s)	1427, 1916
BseNI	ACTGG	5 cut(s)	156, 348, 414, 1232, 2016
BseRI	GAGGAG	2 cut(s)	398, 1401
BseSI	GKGCMC	1 cut(s)	1612
BseXI	GCAGC	7 cut(s)	466, 471, 803, 1093, 1436, 1561, 1951
BseYI	CCCAGC	1 cut(s)	829
BsgI	GTGCAG	1 cut(s)	2422
Bsh1285I	CGRYCG	1 cut(s)	511
BshNI	GGYRCC	2 cut(s)	1799, 2027
BshVI	ATCGAT	1 cut(s)	14
BsiEI	CGRYCG	1 cut(s)	511
BsiHKCI	CYCGRG	2 cut(s)	590, 2423
BsiSI	CCGG	2 cut(s)	728, 1360
BslFI	GGGAC	1 cut(s)	1505
BslI	CCNNNNNNNGG	6 cut(s)	503, 1139, 1873, 2156, 2316, 2560
BsmAI	GTCTC	7 cut(s)	330, 1169, 1836, 1943, 2006, 2401, 2569
BsmBI	CGTCTC	1 cut(s)	1943
BsmFI	GGGAC	1 cut(s)	1505
BsmI	GAATGC	2 cut(s)	831, 1315
Bso31I	GGTCTC	4 cut(s)	330, 2006, 2401, 2569
BsoBI	CYCGRG	2 cut(s)	590, 2423
Bsp119I	TTCGAA	2 cut(s)	2207, 2571
Bsp1286I	GDGCHC	2 cut(s)	82, 1612
Bsp1407I	TGTACA	2 cut(s)	891, 1156
BspACI	CCGC	4 cut(s)	532, 655, 883, 1134
BspCNI	CTCAG	2 cut(s)	1426, 1917
BspDI	ATCGAT	1 cut(s)	14
BspHI	TCATGA	3 cut(s)	613, 1561, 1591
BspLI	GGNNCC	9 cut(s)	225, 1801, 1878, 2029, 2058, 2095, 2161, 2234, 2325
BspMAI	CTGCAG	1 cut(s)	821
BspPI	GGATC	9 cut(s)	218, 231, 1150, 1871, 1884, 2154, 2167, 2193, 2352
BspQI	GCTCTTC	1 cut(s)	87
BspT104I	TTCGAA	2 cut(s)	2207, 2571
BspT107I	GGYRCC	2 cut(s)	1799, 2027
BspTNI	GGTCTC	4 cut(s)	330, 2006, 2401, 2569
BsrDI	GCAATG	1 cut(s)	142
BsrFI	RCCGGY	2 cut(s)	727, 1359
BsrGI	TGTACA	2 cut(s)	891, 1156
BsrI	ACTGG	5 cut(s)	156, 348, 414, 1232, 2016
BssAI	RCCGGY	2 cut(s)	727, 1359
BssECI	CCNNGG	7 cut(s)	74, 86, 502, 591, 1487, 1503, 2172
BssNAI	GTATAC	1 cut(s)	2076
BssNI	GRCGYC	1 cut(s)	5
BssSI	CACGAG	1 cut(s)	1942
BssT1I	CCWWGG	4 cut(s)	74, 86, 1487, 2172
Bst1107I	GTATAC	1 cut(s)	2076
Bst2BI	CACGAG	1 cut(s)	1942
Bst2UI	CCWGG	2 cut(s)	105, 2561
Bst4CI	ACNGT	7 cut(s)	100, 528, 889, 1665, 1891, 2395, 2443
Bst6I	CTCTTC	1 cut(s)	87
BstACI	GRCGYC	1 cut(s)	5
BstAUI	TGTACA	2 cut(s)	891, 1156
BstBI	TTCGAA	2 cut(s)	2207, 2571
BstC8I	GCNNGC	7 cut(s)	452, 534, 1361, 1424, 1642, 2237, 2437
BstDEI	CTNAG	4 cut(s)	1333, 1413, 1925, 1985
BstDSI	CCRYGG	2 cut(s)	502, 1503
BstENI	CCTNNNNNAGG	1 cut(s)	1871
BstF5I	GGATG	6 cut(s)	262, 292, 704, 1327, 2095, 2356
BstH2I	RGCGCY	1 cut(s)	2121
BstHHI	GCGC	2 cut(s)	23, 2120
BstMAI	GTCTC	7 cut(s)	330, 1169, 1836, 1943, 2006, 2401, 2569
BstMCI	CGRYCG	1 cut(s)	511
BstMWI	GCNNNNNNNGC	4 cut(s)	463, 1283, 1607, 2388
BstNI	CCWGG	2 cut(s)	105, 2561
BstNSI	RCATGY	6 cut(s)	215, 484, 1256, 1426, 1644, 2374
BstSCI	CCNGG	2 cut(s)	103, 2559
BstSFI	CTRYAG	4 cut(s)	817, 909, 1287, 1734
BstSLI	GKGCMC	1 cut(s)	1612
BstV1I	GCAGC	7 cut(s)	466, 471, 803, 1093, 1436, 1561, 1951
BstV2I	GAAGAC	1 cut(s)	1277
BstX2I	RGATCY	4 cut(s)	223, 1876, 2159, 2357
BstXI	CCANNNNNNTGG	1 cut(s)	1786
BstYI	RGATCY	4 cut(s)	223, 1876, 2159, 2357
BstZ17I	GTATAC	1 cut(s)	2076
Bsu15I	ATCGAT	1 cut(s)	14
BsuTUI	ATCGAT	1 cut(s)	14
BtgI	CCRYGG	2 cut(s)	502, 1503
BtrI	CACGTC	1 cut(s)	800
BtsCI	GGATG	6 cut(s)	262, 292, 704, 1327, 2095, 2356
BtsIMutI	CAGTG	7 cut(s)	355, 421, 524, 894, 1239, 1494, 2494
Cac8I	GCNNGC	7 cut(s)	452, 534, 1361, 1424, 1642, 2237, 2437
CaiI	CAGNNNCTG	1 cut(s)	376
CciI	TCATGA	3 cut(s)	613, 1561, 1591
CfoI	GCGC	2 cut(s)	23, 2120
Cfr10I	RCCGGY	2 cut(s)	727, 1359
Cfr13I	GGNCC	6 cut(s)	534, 730, 1237, 1776, 2233, 2323
ClaI	ATCGAT	1 cut(s)	14
CpoI	CGGWCCG	1 cut(s)	730
CseI	GACGC	1 cut(s)	1960
Csp6I	GTAC	8 cut(s)	69, 398, 658, 892, 1157, 1800, 1978, 2470
CspI	CGGWCCG	1 cut(s)	730
CviQI	GTAC	8 cut(s)	69, 398, 658, 892, 1157, 1800, 1978, 2470
DdeI	CTNAG	4 cut(s)	1333, 1413, 1925, 1985
DraI	TTTAAA	1 cut(s)	1039
EaeI	YGGCCR	2 cut(s)	880, 2307
Eam1104I	CTCTTC	1 cut(s)	87
EarI	CTCTTC	1 cut(s)	87
Eco130I	CCWWGG	4 cut(s)	74, 86, 1487, 2172
Eco147I	AGGCCT	2 cut(s)	51, 2514
Eco24I	GRGCYC	1 cut(s)	82
Eco31I	GGTCTC	4 cut(s)	330, 2006, 2401, 2569
Eco47I	GGWCC	2 cut(s)	730, 1237
Eco47III	AGCGCT	1 cut(s)	2119
Eco57I	CTGAAG	1 cut(s)	1833
Eco88I	CYCGRG	2 cut(s)	590, 2423
EcoNI	CCTNNNNNAGG	1 cut(s)	1871
EcoO109I	RGGNCCY	1 cut(s)	2323
EcoRI	GAATTC	1 cut(s)	1861
EcoRII	CCWGG	2 cut(s)	103, 2559
EcoT14I	CCWWGG	4 cut(s)	74, 86, 1487, 2172
EcoT22I	ATGCAT	1 cut(s)	1516
EcoT38I	GRGCYC	1 cut(s)	82
ErhI	CCWWGG	4 cut(s)	74, 86, 1487, 2172
Esp3I	CGTCTC	1 cut(s)	1943
FaqI	GGGAC	1 cut(s)	1505
FauI	CCCGC	2 cut(s)	525, 1141
FauNDI	CATATG	1 cut(s)	921
FblI	GTMKAC	1 cut(s)	2075
Fnu4HI	GCNGC	8 cut(s)	455, 485, 817, 883, 1082, 1450, 1550, 1965
FokI	GGATG	6 cut(s)	269, 299, 691, 1334, 2102, 2363
FriOI	GRGCYC	1 cut(s)	82
Fsp4HI	GCNGC	8 cut(s)	455, 485, 817, 883, 1082, 1450, 1550, 1965
FspBI	CTAG	2 cut(s)	674, 2400
FspI	TGCGCA	1 cut(s)	22
GlaI	GCGC	2 cut(s)	22, 2119
GluI	GCNGC	8 cut(s)	455, 485, 817, 883, 1082, 1450, 1550, 1965
GsaI	CCCAGC	1 cut(s)	833
GsuI	CTGGAG	3 cut(s)	66, 401, 407
HaeII	RGCGCY	1 cut(s)	2121
HapII	CCGG	2 cut(s)	728, 1360
HgaI	GACGC	1 cut(s)	1960
HhaI	GCGC	2 cut(s)	23, 2120
Hin1I	GRCGYC	1 cut(s)	5
Hin6I	GCGC	2 cut(s)	21, 2118
HinP1I	GCGC	2 cut(s)	21, 2118
HincII	GTYRAC	1 cut(s)	1483
HindII	GTYRAC	1 cut(s)	1483
HindIII	AAGCTT	2 cut(s)	1615, 1815
HpaI	GTTAAC	1 cut(s)	1483
HpaII	CCGG	2 cut(s)	728, 1360
HphI	GGTGA	8 cut(s)	103, 152, 583, 1209, 1446, 1522, 2126, 2409
Hpy166II	GTNNAC	7 cut(s)	353, 398, 892, 1217, 1483, 1661, 2076
Hpy8I	GTNNAC	7 cut(s)	353, 398, 892, 1217, 1483, 1661, 2076
Hpy99I	CGWCG	2 cut(s)	2366, 2447
HpyAV	CCTTC	8 cut(s)	277, 695, 1188, 1467, 1767, 1908, 2314, 2424
HpyCH4III	ACNGT	7 cut(s)	100, 528, 889, 1665, 1891, 2395, 2443
HpyCH4IV	ACGT	4 cut(s)	404, 641, 799, 1558
HpyF10VI	GCNNNNNNNGC	4 cut(s)	463, 1283, 1607, 2388
HpyF3I	CTNAG	4 cut(s)	1333, 1413, 1925, 1985
HpySE526I	ACGT	4 cut(s)	404, 641, 799, 1558
Hsp92I	GRCGYC	1 cut(s)	5
HspAI	GCGC	2 cut(s)	21, 2118
KpnI	GGTACC	1 cut(s)	1803
KroI	GCCGGC	1 cut(s)	1359
KroNI	GCCGGC	1 cut(s)	1361
KspAI	GTTAAC	1 cut(s)	1483
LguI	GCTCTTC	1 cut(s)	87
LmnI	GCTCC	3 cut(s)	1469, 2056, 2099
Lsp1109I	GCAGC	7 cut(s)	466, 471, 803, 1093, 1436, 1561, 1951
LweI	GCATC	7 cut(s)	50, 142, 1489, 1501, 2131, 2140, 2554
MaeI	CTAG	2 cut(s)	674, 2400
MaeII	ACGT	4 cut(s)	404, 641, 799, 1558
MaeIII	GTNAC	6 cut(s)	140, 201, 273, 800, 1554, 2502
MboII	GAAGA	8 cut(s)	74, 247, 371, 439, 673, 941, 1282, 2460
MfeI	CAATTG	1 cut(s)	391
MflI	RGATCY	4 cut(s)	223, 1876, 2159, 2357
MhlI	GDGCHC	2 cut(s)	82, 1612
MlsI	TGGCCA	1 cut(s)	2309
MluNI	TGGCCA	1 cut(s)	2309
MmeI	TCCRAC	4 cut(s)	2121, 2163, 2385, 2425
Mox20I	TGGCCA	1 cut(s)	2309
Mph1103I	ATGCAT	1 cut(s)	1516
MroNI	GCCGGC	1 cut(s)	1359
MroXI	GAANNNNTTC	3 cut(s)	1918, 2199, 2292
MscI	TGGCCA	1 cut(s)	2309
MslI	CAYNNNNRTG	1 cut(s)	2139
Msp20I	TGGCCA	1 cut(s)	2309
MspI	CCGG	2 cut(s)	728, 1360
MspR9I	CCNGG	2 cut(s)	105, 2561
MunI	CAATTG	1 cut(s)	391
Mva1269I	GAATGC	2 cut(s)	831, 1315
MvaI	CCWGG	2 cut(s)	105, 2561
MwoI	GCNNNNNNNGC	4 cut(s)	463, 1283, 1607, 2388
NaeI	GCCGGC	1 cut(s)	1361
NdeI	CATATG	1 cut(s)	921
NgoMIV	GCCGGC	1 cut(s)	1359
NlaIV	GGNNCC	9 cut(s)	225, 1801, 1878, 2029, 2058, 2095, 2161, 2234, 2325
NmeAIII	GCCGAG	1 cut(s)	1670
NmuCI	GTSAC	2 cut(s)	140, 800
NsbI	TGCGCA	1 cut(s)	22
NsiI	ATGCAT	1 cut(s)	1516
NspI	RCATGY	6 cut(s)	215, 484, 1256, 1426, 1644, 2374
NspV	TTCGAA	2 cut(s)	2207, 2571
PaeI	GCATGC	2 cut(s)	1426, 1644
PagI	TCATGA	3 cut(s)	613, 1561, 1591
PceI	AGGCCT	2 cut(s)	51, 2514
PciI	ACATGT	2 cut(s)	1252, 2370
PciSI	GCTCTTC	1 cut(s)	87
PctI	GAATGC	2 cut(s)	831, 1315
PdiI	GCCGGC	1 cut(s)	1361
PdmI	GAANNNNTTC	3 cut(s)	1918, 2199, 2292
PkrI	GCNGC	8 cut(s)	456, 486, 818, 884, 1083, 1451, 1551, 1966
PscI	ACATGT	2 cut(s)	1252, 2370
PshBI	ATTAAT	1 cut(s)	585
PsiI	TTATAA	1 cut(s)	1127
Psp1406I	AACGTT	2 cut(s)	404, 641
Psp6I	CCWGG	2 cut(s)	103, 2559
PspFI	CCCAGC	1 cut(s)	829
PspGI	CCWGG	2 cut(s)	103, 2559
PspN4I	GGNNCC	9 cut(s)	225, 1801, 1878, 2029, 2058, 2095, 2161, 2234, 2325
PspPI	GGNCC	6 cut(s)	534, 730, 1237, 1776, 2233, 2323
PstI	CTGCAG	1 cut(s)	821
PstNI	CAGNNNCTG	1 cut(s)	376
PsuI	RGATCY	4 cut(s)	223, 1876, 2159, 2357
RsaI	GTAC	8 cut(s)	70, 399, 659, 893, 1158, 1801, 1979, 2471
RsaNI	GTAC	8 cut(s)	69, 398, 658, 892, 1157, 1800, 1978, 2470
RseI	CAYNNNNRTG	1 cut(s)	2139
Rsr2I	CGGWCCG	1 cut(s)	730
RsrII	CGGWCCG	1 cut(s)	730
SapI	GCTCTTC	1 cut(s)	87
SatI	GCNGC	8 cut(s)	455, 485, 817, 883, 1082, 1450, 1550, 1965
Sau96I	GGNCC	6 cut(s)	534, 730, 1237, 1776, 2233, 2323
ScaI	AGTACT	1 cut(s)	2471
ScrFI	CCNGG	2 cut(s)	105, 2561
SduI	GDGCHC	2 cut(s)	82, 1612
SfaNI	GCATC	7 cut(s)	50, 142, 1489, 1501, 2131, 2140, 2554
SfcI	CTRYAG	4 cut(s)	817, 909, 1287, 1734
SfuI	TTCGAA	2 cut(s)	2207, 2571
SinI	GGWCC	2 cut(s)	730, 1237
SmiMI	CAYNNNNRTG	1 cut(s)	2139
SmlI	CTYRAG	2 cut(s)	329, 1687
SmoI	CTYRAG	2 cut(s)	329, 1687
SphI	GCATGC	2 cut(s)	1426, 1644
SseBI	AGGCCT	2 cut(s)	51, 2514
SsiI	CCGC	4 cut(s)	532, 655, 883, 1134
SspI	AATATT	1 cut(s)	558
SspMI	CTAG	2 cut(s)	674, 2400
StuI	AGGCCT	2 cut(s)	51, 2514
StyD4I	CCNGG	2 cut(s)	103, 2559
StyI	CCWWGG	4 cut(s)	74, 86, 1487, 2172
TaaI	ACNGT	7 cut(s)	100, 528, 889, 1665, 1891, 2395, 2443
TaiI	ACGT	4 cut(s)	407, 644, 802, 1561
TaqII	GACCGA	1 cut(s)	525
TatI	WGTACW	4 cut(s)	68, 891, 1156, 2469
TauI	GCSGC	1 cut(s)	885
TscAI	CASTG	7 cut(s)	355, 421, 531, 894, 1239, 1501, 2494
TseFI	GTSAC	2 cut(s)	140, 800
TseI	GCWGC	7 cut(s)	454, 484, 816, 1081, 1449, 1549, 1964
Tsp45I	GTSAC	2 cut(s)	140, 800
TspGWI	ACGGA	2 cut(s)	722, 2102
TspRI	CASTG	7 cut(s)	355, 421, 531, 894, 1239, 1501, 2494
VpaK11BI	GGWCC	2 cut(s)	730, 1237
VspI	ATTAAT	1 cut(s)	585
XagI	CCTNNNNNAGG	1 cut(s)	1871
XapI	RAATTY	6 cut(s)	711, 741, 900, 1439, 1861, 2292
XceI	RCATGY	6 cut(s)	215, 484, 1256, 1426, 1644, 2374
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2179
XmiI	GTMKAC	1 cut(s)	2075
XmnI	GAANNNNTTC	3 cut(s)	1918, 2199, 2292
XspI	CTAG	2 cut(s)	674, 2400
ZrmI	AGTACT	1 cut(s)	2471
Zsp2I	ATGCAT	1 cut(s)	1516

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.