pycom16g13680
MYB Family

Two-component response regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0003243
Physical Location & Seq
Forward (+)
9766651 .. 9767904
1254 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 654 bp
ATGCATATGAGCAACGGCATGCGATCGATGTCAACAGCTAGTTCGAGTGGAGCTTGGAAATCGGGGGATGTGGTCACTGACCAGTTTCCTGCGGGACTAAGAGTCCTTGTGGTCGACGATGATCCAACTTGCCTTATGATCTTGGAGAGGATGCTCAGAACTTGCCTCTATGAAGTGACAAAATGTAATCGTGCGGAGATCGCACTTTCGCTGCTTCGAGAGAACCAGAACGGATTCGATATTGTTATCAGTGATGTGCACATGCCAGACATGGATGGATTCAAACTCCTAGAGCACGTTGGACTGGAGATGGACCTGCCTGTTATCATGATGTCTGCCGATGATGGAAAAAGTGTTGTTATGAAGGGTGTGACTCATGGTGCTTGTGATTACCTAATCAAACCGGTTCGCATTGAGGCGCTGAAGAACATATGGCAGCATGTAGTGCGGAAGAAAAAGAATGAGTGGAAGGATGTGGAGCAATCTGGAAGTGTGGAAGAAGCGGGGATCGGCAGCAGAAACCATCCGAAGATTCAGATTACTCGTCCTCGGCAAATGAAGGGACTTGGAAGAACTCAAAGAGGAGGAAGGATGAGGATGAAGAGACGGATGAGAGGGATGAATTCGAATTTTTTTCTGCGGTCTCGCATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000156 GO:0000160 GO:0001101 GO:0001763 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0009414 GO:0009415 GO:0009628 GO:0009653 GO:0009719 GO:0009723 GO:0009725 GO:0009735 GO:0009736 GO:0009755 GO:0009873 GO:0009888 GO:0009889 GO:0009933 GO:0009966 GO:0009987 GO:0010014 GO:0010015 GO:0010016 GO:0010033 GO:0010035 GO:0010073 GO:0010074 GO:0010075 GO:0010082 GO:0010119 GO:0010150 GO:0010223 GO:0010346 GO:0010380 GO:0010468 GO:0010492 GO:0010556 GO:0010646 GO:0019219 GO:0019222 GO:0019827 GO:0022622 GO:0023051 GO:0023052 GO:0031323 GO:0031326 GO:0031537 GO:0032501 GO:0032502 GO:0032870 GO:0035556 GO:0040008 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048364 GO:0048366 GO:0048367 GO:0048507 GO:0048509 GO:0048532 GO:0048580 GO:0048583 GO:0048638 GO:0048646 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051193 GO:0051239 GO:0051252 GO:0051716 GO:0060089 GO:0060255 GO:0065007 GO:0070887 GO:0071310 GO:0071368 GO:0071369 GO:0071495 GO:0080022 GO:0080036 GO:0080050 GO:0080090 GO:0080113 GO:0090056 GO:0090506 GO:0090693 GO:0098727 GO:0099402 GO:0140110 GO:1901401 GO:1901463 GO:1901700 GO:1903506 GO:1905392 GO:1905393 GO:2000026 GO:2000112 GO:2000241 GO:2000280 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Myb_DNA-binding Response_reg

Protein Analysis

218

Amino Acids

24.59

Weight (kDa)

9.62

Isoelectric Point (pI)

43.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Response_reg PF00072 35 - 143 3.2e-23 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 324
AccI GTMKAC 1 cut(s) 114
AciI CCGC 5 cut(s) 92, 194, 448, 503, 640
AclWI GGATC 2 cut(s) 116, 515
AcsI RAATTY 2 cut(s) 622, 628
AcuI CTGAAG 1 cut(s) 443
AgeI ACCGGT 1 cut(s) 403
AgsI TTSAA 1 cut(s) 283
AhdI GACNNNNNGTC 1 cut(s) 101
AluBI AGCT 2 cut(s) 38, 53
AluI AGCT 2 cut(s) 38, 53
Alw21I GWGCWC 2 cut(s) 261, 297
Alw26I GTCTC 2 cut(s) 598, 648
Alw44I GTGCAC 1 cut(s) 257
AlwI GGATC 2 cut(s) 116, 515
ApaLI GTGCAC 1 cut(s) 257
ApeKI GCWGC 3 cut(s) 211, 436, 513
ApoI RAATTY 2 cut(s) 622, 628
AsiGI ACCGGT 1 cut(s) 403
Asp700I GAANNNNTTC 1 cut(s) 233
AspLEI GCGC 1 cut(s) 421
AspS9I GGNCC 1 cut(s) 313
AsuII TTCGAA 1 cut(s) 626
AvaII GGWCC 1 cut(s) 313
BaeGI GKGCMC 1 cut(s) 261
Bbv12I GWGCWC 2 cut(s) 261, 297
BbvI GCAGC 3 cut(s) 198, 448, 525
BccI CCATC 4 cut(s) 269, 304, 338, 531
BceAI ACGGC 1 cut(s) 31
BcoDI GTCTC 2 cut(s) 598, 648
BfaI CTAG 2 cut(s) 39, 290
BfoI RGCGCY 1 cut(s) 422
BfuAI ACCTGC 1 cut(s) 324
BisI GCNGC 3 cut(s) 212, 437, 514
BlsI GCNGC 3 cut(s) 213, 438, 515
Bme18I GGWCC 1 cut(s) 313
BmeRI GACNNNNNGTC 1 cut(s) 101
BmgT120I GGNCC 1 cut(s) 313
BmsI GCATC 1 cut(s) 141
BpmI CTGGAG 1 cut(s) 326
Bpu14I TTCGAA 1 cut(s) 626
Bsa29I ATCGAT 1 cut(s) 26
BsaI GGTCTC 1 cut(s) 648
BsaJI CCNNGG 1 cut(s) 548
BsaWI WCCGGW 1 cut(s) 403
Bse118I RCCGGY 1 cut(s) 403
Bse1I ACTGG 2 cut(s) 82, 309
BseCI ATCGAT 1 cut(s) 26
BseDI CCNNGG 1 cut(s) 548
BseGI GGATG 9 cut(s) 73, 156, 280, 478, 523, 597, 603, 615, 624
BseMII CTCAG 1 cut(s) 169
BseNI ACTGG 2 cut(s) 82, 309
BseRI GAGGAG 1 cut(s) 597
BseSI GKGCMC 1 cut(s) 261
BseXI GCAGC 3 cut(s) 198, 448, 525
Bsh1285I CGRYCG 1 cut(s) 26
BshTI ACCGGT 1 cut(s) 403
BshVI ATCGAT 1 cut(s) 26
BsiEI CGRYCG 1 cut(s) 26
BsiHKAI GWGCWC 2 cut(s) 261, 297
BsiSI CCGG 1 cut(s) 404
BslFI GGGAC 2 cut(s) 108, 576
BsmAI GTCTC 2 cut(s) 598, 648
BsmBI CGTCTC 1 cut(s) 598
BsmFI GGGAC 2 cut(s) 108, 576
Bso31I GGTCTC 1 cut(s) 648
Bsp119I TTCGAA 1 cut(s) 626
Bsp1286I GDGCHC 2 cut(s) 261, 297
Bsp143I GATC 5 cut(s) 23, 121, 138, 198, 507
BspACI CCGC 5 cut(s) 92, 194, 448, 503, 640
BspCNI CTCAG 1 cut(s) 168
BspDI ATCGAT 1 cut(s) 26
BspHI TCATGA 1 cut(s) 327
BspMI ACCTGC 1 cut(s) 324
BspPI GGATC 2 cut(s) 116, 515
BspT104I TTCGAA 1 cut(s) 626
BspTNI GGTCTC 1 cut(s) 648
BsrFI RCCGGY 1 cut(s) 403
BsrI ACTGG 2 cut(s) 82, 309
BssAI RCCGGY 1 cut(s) 403
BssECI CCNNGG 1 cut(s) 548
BssMI GATC 5 cut(s) 23, 121, 138, 198, 507
Bst6I CTCTTC 1 cut(s) 596
BstAPI GCANNNNNTGC 1 cut(s) 445
BstBI TTCGAA 1 cut(s) 626
BstC8I GCNNGC 1 cut(s) 20
BstDEI CTNAG 2 cut(s) 98, 155
BstF5I GGATG 9 cut(s) 73, 156, 280, 478, 523, 597, 603, 615, 624
BstH2I RGCGCY 1 cut(s) 422
BstHHI GCGC 1 cut(s) 421
BstKTI GATC 5 cut(s) 26, 124, 141, 201, 510
BstMAI GTCTC 2 cut(s) 598, 648
BstMBI GATC 5 cut(s) 23, 121, 138, 198, 507
BstMCI CGRYCG 1 cut(s) 26
BstMWI GCNNNNNNNGC 2 cut(s) 200, 445
BstNSI RCATGY 3 cut(s) 22, 265, 443
BstSLI GKGCMC 1 cut(s) 261
BstV1I GCAGC 3 cut(s) 198, 448, 525
Bsu15I ATCGAT 1 cut(s) 26
BsuTUI ATCGAT 1 cut(s) 26
BtsCI GGATG 9 cut(s) 73, 156, 280, 478, 523, 597, 603, 615, 624
BtsIMutI CAGTG 2 cut(s) 75, 256
BveI ACCTGC 1 cut(s) 324
Cac8I GCNNGC 1 cut(s) 20
CciI TCATGA 1 cut(s) 327
CfoI GCGC 1 cut(s) 421
Cfr10I RCCGGY 1 cut(s) 403
Cfr13I GGNCC 1 cut(s) 313
ClaI ATCGAT 1 cut(s) 26
CspAI ACCGGT 1 cut(s) 403
CviAII CATG 6 cut(s) 19, 262, 271, 328, 377, 440
CviJI RGCY 2 cut(s) 38, 53
CviKI_1 RGCY 2 cut(s) 38, 53
DdeI CTNAG 2 cut(s) 98, 155
DpnI GATC 5 cut(s) 25, 123, 140, 200, 509
DpnII GATC 5 cut(s) 23, 121, 138, 198, 507
DriI GACNNNNNGTC 1 cut(s) 101
Eam1104I CTCTTC 1 cut(s) 596
Eam1105I GACNNNNNGTC 1 cut(s) 101
EarI CTCTTC 1 cut(s) 596
Eco31I GGTCTC 1 cut(s) 648
Eco47I GGWCC 1 cut(s) 313
Eco57I CTGAAG 1 cut(s) 443
EcoRI GAATTC 1 cut(s) 622
EcoT22I ATGCAT 1 cut(s) 6
Esp3I CGTCTC 1 cut(s) 598
FaeI CATG 6 cut(s) 22, 265, 274, 331, 380, 443
FaqI GGGAC 2 cut(s) 108, 576
FatI CATG 6 cut(s) 18, 261, 270, 327, 376, 439
FauI CCCGC 2 cut(s) 85, 496
FauNDI CATATG 2 cut(s) 6, 431
FblI GTMKAC 1 cut(s) 114
Fnu4HI GCNGC 3 cut(s) 212, 437, 514
FokI GGATG 9 cut(s) 80, 163, 287, 485, 510, 604, 610, 622, 631
Fsp4HI GCNGC 3 cut(s) 212, 437, 514
FspBI CTAG 2 cut(s) 39, 290
GlaI GCGC 1 cut(s) 420
GluI GCNGC 3 cut(s) 212, 437, 514
GsuI CTGGAG 1 cut(s) 326
HaeII RGCGCY 1 cut(s) 422
HapII CCGG 1 cut(s) 404
HhaI GCGC 1 cut(s) 421
Hin1II CATG 6 cut(s) 22, 265, 274, 331, 380, 443
Hin6I GCGC 1 cut(s) 419
HinP1I GCGC 1 cut(s) 419
HincII GTYRAC 2 cut(s) 33, 115
HindII GTYRAC 2 cut(s) 33, 115
HinfI GANTC 5 cut(s) 102, 234, 279, 373, 532
HpaII CCGG 1 cut(s) 404
Hpy166II GTNNAC 3 cut(s) 33, 115, 259
Hpy188I TCNGA 3 cut(s) 158, 528, 537
Hpy188III TCNNGA 3 cut(s) 218, 328, 486
Hpy8I GTNNAC 3 cut(s) 33, 115, 259
Hpy99I CGWCG 1 cut(s) 119
HpyAV CCTTC 4 cut(s) 358, 463, 553, 582
HpyCH4IV ACGT 1 cut(s) 297
HpyCH4V TGCA 2 cut(s) 4, 259
HpyF10VI GCNNNNNNNGC 2 cut(s) 200, 445
HpyF3I CTNAG 2 cut(s) 98, 155
HpySE526I ACGT 1 cut(s) 297
Hsp92II CATG 6 cut(s) 22, 265, 274, 331, 380, 443
HspAI GCGC 1 cut(s) 419
Kzo9I GATC 5 cut(s) 23, 121, 138, 198, 507
LmnI GCTCC 2 cut(s) 50, 478
LpnPI CCDG 9 cut(s) 95, 102, 239, 279, 290, 329, 333, 417, 471
Lsp1109I GCAGC 3 cut(s) 198, 448, 525
LweI GCATC 1 cut(s) 141
MaeI CTAG 2 cut(s) 39, 290
MaeII ACGT 1 cut(s) 297
MaeIII GTNAC 3 cut(s) 73, 175, 370
MalI GATC 5 cut(s) 25, 123, 140, 200, 509
MboI GATC 5 cut(s) 23, 121, 138, 198, 507
MboII GAAGA 6 cut(s) 436, 463, 509, 541, 582, 613
MhlI GDGCHC 2 cut(s) 261, 297
MluCI AATT 2 cut(s) 622, 628
MlyI GAGTC 2 cut(s) 111, 367
MmeI TCCRAC 2 cut(s) 149, 280
MnlI CCTC 8 cut(s) 141, 176, 409, 558, 575, 578, 588, 608
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 233
MspI CCGG 1 cut(s) 404
MwoI GCNNNNNNNGC 2 cut(s) 200, 445
NdeI CATATG 2 cut(s) 6, 431
NdeII GATC 5 cut(s) 23, 121, 138, 198, 507
NlaIII CATG 6 cut(s) 22, 265, 274, 331, 380, 443
NmeAIII GCCGAG 1 cut(s) 529
NmuCI GTSAC 3 cut(s) 73, 175, 370
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 3 cut(s) 22, 265, 443
NspV TTCGAA 1 cut(s) 626
PaeI GCATGC 1 cut(s) 22
PagI TCATGA 1 cut(s) 327
PdmI GAANNNNTTC 1 cut(s) 233
PfeI GAWTC 3 cut(s) 234, 279, 532
PinAI ACCGGT 1 cut(s) 403
PkrI GCNGC 3 cut(s) 213, 438, 515
Ple19I CGATCG 1 cut(s) 26
PleI GAGTC 2 cut(s) 110, 367
PpsI GAGTC 2 cut(s) 110, 367
PspPI GGNCC 1 cut(s) 313
PvuI CGATCG 1 cut(s) 26
SalI GTCGAC 1 cut(s) 113
SatI GCNGC 3 cut(s) 212, 437, 514
Sau3AI GATC 5 cut(s) 23, 121, 138, 198, 507
Sau96I GGNCC 1 cut(s) 313
SchI GAGTC 2 cut(s) 111, 367
SduI GDGCHC 2 cut(s) 261, 297
SetI ASST 5 cut(s) 40, 55, 300, 318, 396
SfaNI GCATC 1 cut(s) 141
SfuI TTCGAA 1 cut(s) 626
SinI GGWCC 1 cut(s) 313
SphI GCATGC 1 cut(s) 22
Sse9I AATT 2 cut(s) 622, 628
SsiI CCGC 5 cut(s) 92, 194, 448, 503, 640
SspMI CTAG 2 cut(s) 39, 290
TaiI ACGT 1 cut(s) 300
TaqI TCGA 6 cut(s) 26, 44, 114, 217, 237, 626
TasI AATT 2 cut(s) 622, 628
TfiI GAWTC 3 cut(s) 234, 279, 532
TscAI CASTG 2 cut(s) 82, 256
TseFI GTSAC 3 cut(s) 73, 175, 370
TseI GCWGC 3 cut(s) 211, 436, 513
Tsp45I GTSAC 3 cut(s) 73, 175, 370
TspDTI ATGAA 5 cut(s) 186, 377, 572, 614, 635
TspGWI ACGGA 2 cut(s) 246, 622
TspRI CASTG 2 cut(s) 82, 256
VneI GTGCAC 1 cut(s) 257
VpaK11BI GGWCC 1 cut(s) 313
XapI RAATTY 2 cut(s) 622, 628
XceI RCATGY 3 cut(s) 22, 265, 443
XmiI GTMKAC 1 cut(s) 114
XmnI GAANNNNTTC 1 cut(s) 233
XspI CTAG 2 cut(s) 39, 290
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.