pycom16g14600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0009250
Physical Location & Seq
Forward (+)
10677557 .. 10680795
3239 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1206 bp
ATGCAAGTGATCTCCAACACTCGCAGGCTCTCCAGAGCTTTCAAATCTCCCATTTTTATCAAATCCGCCGACCAATTTCTCTCCCCCGATGCCCGAGTCGTCGAAGCTCCACTCACTTCTCTCCAATGGCGGAACCTCAGCGCCGGCTCTTCCCAAACTGCCTTGTTATCCGGCATTACCCGCTGTCATCTTTTGCCGATGGAACAACATGCAACGTGTGCAGTGATGACGTTGAGAGCACCCTTTTCTTCTGACGCAAGTACCATCGAAGGTGGTTCCACAGAGGTTGTGAAGGAGCTGTATGATAAAATGCTGCAGTCTGTTAATATCAAACGATCAATGCCCCCTAATGCTTGGTTATGGTCATTGATTGAAAATTGCAAAAAGGAAGAAGATATTAAGCTTCTATTTGACATCTTGCAGAACCTCCGAAGATTTAGACTGTCAAATCTTCGCATCCATTCCGACTTTAATTGCAACCTCTGCCGAGAGGTTACTAAGGCATGTGTTCGTGTGGGAGCTCTTGACTTTGGAAAGAAGGCCTTGTGGAAACATAACGTGTATGGATTGGATCCTAGCATCGGATCTGCGCACCATTTACTGTTGTATGCTAAAGAACGCAATGATGCTAAACTTATGGTGGAAGTAATGAAACTTTTGAAGAGGAATGACTTACCCTTGCAACCTGGTACAGCAGATATTGTTTTCAGCATTTGTTACAATACTGATAATTGGCAGCTGATGTCAAAGTACTCAAAAAGGTTTGTCAAAGCTGGAGTAAAGCTACGTCAAACTGCTTTTGATTCATGGATGGATTTTGCTGCTAAAATAGGAGATGTTGAATCATTGTGGAAAGCTGAGAAGTTAAGATCTGAATCGATGAAGCAGCATACTATTGCAAGTGGACTTTCATGTGCTAAGGGTTTTCTCTTAGAGCGTAAGCCCGAGGAAGCTGCTTCTGTTATTCAAATTCTAAATCAGATTTTACCTGATACAAAGAAGTCTAGCGTTGTGGTCGAGCTTGAAAAGTTGGTTAATGAGTGGCCCTTAGAGGTTATTAGGCACCAGAAGAAAGAGGACAGAAAGGCATTAGCTACATCTTTAATCGCTGATATCCCTACAATGGTTAGTTCGCTATTAAAGACGGGCTTACAAGTGAGCGCAAGCATTGAGGACCTAACCACCAAAGAAGGTATTCTTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

402

Amino Acids

45.21

Weight (kDa)

9.24

Isoelectric Point (pI)

49.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 591
AccB1I GGYRCC 1 cut(s) 1062
AciI CCGC 3 cut(s) 66, 130, 181
AclWI GGATC 3 cut(s) 566, 579, 592
AcsI RAATTY 1 cut(s) 969
AfaI GTAC 3 cut(s) 262, 691, 752
AfiI CCNNNNNNNGG 2 cut(s) 581, 1123
AflIII ACRYGT 2 cut(s) 215, 558
AgsI TTSAA 6 cut(s) 43, 374, 661, 842, 968, 1025
AjnI CCWGG 1 cut(s) 685
Alw21I GWGCWC 2 cut(s) 241, 523
AlwI GGATC 3 cut(s) 566, 579, 592
Ama87I CYCGRG 2 cut(s) 93, 944
AoxI GGCC 2 cut(s) 540, 1043
ApeKI GCWGC 5 cut(s) 313, 736, 821, 886, 953
ApoI RAATTY 1 cut(s) 969
Asp700I GAANNNNTTC 1 cut(s) 1194
AspLEI GCGC 3 cut(s) 143, 592, 1163
AspS9I GGNCC 2 cut(s) 1044, 1174
AvaI CYCGRG 2 cut(s) 93, 944
AvaII GGWCC 1 cut(s) 1174
BaeI ACNNNNGTAYC 2 cut(s) 244, 277
BamHI GGATCC 1 cut(s) 571
BanI GGYRCC 1 cut(s) 1062
BanII GRGCYC 1 cut(s) 523
Bbv12I GWGCWC 2 cut(s) 241, 523
BbvCI CCTCAGC 1 cut(s) 137
BbvI GCAGC 5 cut(s) 300, 748, 808, 898, 940
BccI CCATC 3 cut(s) 193, 272, 805
BcgI CGANNNNNNTGC 2 cut(s) 935, 969
BciT130I CCWGG 1 cut(s) 687
BfaI CTAG 2 cut(s) 576, 1005
BfmI CTRYAG 1 cut(s) 314
BfoI RGCGCY 1 cut(s) 144
BglII AGATCT 1 cut(s) 869
BisI GCNGC 5 cut(s) 314, 737, 822, 887, 954
BlsI GCNGC 5 cut(s) 315, 738, 823, 888, 955
BmcAI AGTACT 1 cut(s) 752
Bme1390I CCNGG 1 cut(s) 687
Bme18I GGWCC 1 cut(s) 1174
BmeT110I CYCGRG 2 cut(s) 93, 944
BmgT120I GGNCC 2 cut(s) 1044, 1174
BmiI GGNNCC 4 cut(s) 134, 277, 573, 1064
BmrFI CCNGG 1 cut(s) 687
BmsI GCATC 4 cut(s) 79, 465, 588, 616
BpmI CTGGAG 2 cut(s) 16, 795
Bpu10I CCTNAGC 2 cut(s) 137, 918
Bsa29I ATCGAT 1 cut(s) 878
BsaBI GATNNNNATC 1 cut(s) 874
BsaJI CCNNGG 1 cut(s) 945
Bsc4I CCNNNNNNNGG 2 cut(s) 581, 1123
Bse118I RCCGGY 1 cut(s) 143
Bse3DI GCAATG 1 cut(s) 628
Bse8I GATNNNNATC 1 cut(s) 874
BseBI CCWGG 1 cut(s) 687
BseCI ATCGAT 1 cut(s) 878
BseDI CCNNGG 1 cut(s) 945
BseGI GGATG 2 cut(s) 456, 816
BseJI GATNNNNATC 1 cut(s) 874
BseLI CCNNNNNNNGG 2 cut(s) 581, 1123
BseMI GCAATG 1 cut(s) 628
BseMII CTCAG 2 cut(s) 151, 849
BseXI GCAGC 5 cut(s) 300, 748, 808, 898, 940
BsgI GTGCAG 1 cut(s) 240
BshFI GGCC 2 cut(s) 542, 1045
BshNI GGYRCC 1 cut(s) 1062
BshVI ATCGAT 1 cut(s) 878
BsiHKAI GWGCWC 2 cut(s) 241, 523
BsiHKCI CYCGRG 2 cut(s) 93, 944
BsiSI CCGG 2 cut(s) 144, 171
BslI CCNNNNNNNGG 2 cut(s) 581, 1123
BsnI GGCC 2 cut(s) 542, 1045
BsoBI CYCGRG 2 cut(s) 93, 944
Bsp1286I GDGCHC 2 cut(s) 241, 523
Bsp143I GATC 5 cut(s) 9, 335, 571, 584, 869
BspACI CCGC 3 cut(s) 66, 130, 181
BspANI GGCC 2 cut(s) 542, 1045
BspCNI CTCAG 2 cut(s) 150, 850
BspDI ATCGAT 1 cut(s) 878
BspLI GGNNCC 4 cut(s) 134, 277, 573, 1064
BspMAI CTGCAG 1 cut(s) 318
BspPI GGATC 3 cut(s) 566, 579, 592
BspQI GCTCTTC 1 cut(s) 154
BspT107I GGYRCC 1 cut(s) 1062
BsrDI GCAATG 1 cut(s) 628
BsrFI RCCGGY 1 cut(s) 143
BssAI RCCGGY 1 cut(s) 143
BssECI CCNNGG 1 cut(s) 945
BssMI GATC 5 cut(s) 9, 335, 571, 584, 869
Bst2UI CCWGG 1 cut(s) 687
Bst4CI ACNGT 2 cut(s) 444, 603
Bst6I CTCTTC 2 cut(s) 154, 656
BstAPI GCANNNNNTGC 2 cut(s) 218, 483
BstC8I GCNNGC 3 cut(s) 26, 145, 1165
BstDEI CTNAG 6 cut(s) 137, 498, 858, 918, 931, 1048
BstF5I GGATG 2 cut(s) 456, 816
BstH2I RGCGCY 1 cut(s) 144
BstHHI GCGC 3 cut(s) 143, 592, 1163
BstKTI GATC 5 cut(s) 12, 338, 574, 587, 872
BstMBI GATC 5 cut(s) 9, 335, 571, 584, 869
BstMWI GCNNNNNNNGC 3 cut(s) 180, 218, 483
BstNI CCWGG 1 cut(s) 687
BstNSI RCATGY 2 cut(s) 212, 507
BstSCI CCNGG 1 cut(s) 685
BstSFI CTRYAG 1 cut(s) 314
BstV1I GCAGC 5 cut(s) 300, 748, 808, 898, 940
BstX2I RGATCY 3 cut(s) 571, 584, 869
BstYI RGATCY 3 cut(s) 571, 584, 869
Bsu15I ATCGAT 1 cut(s) 878
BsuRI GGCC 2 cut(s) 542, 1045
BsuTUI ATCGAT 1 cut(s) 878
BtsCI GGATG 2 cut(s) 456, 816
BtsI GCAGTG 1 cut(s) 228
BtsIMutI CAGTG 1 cut(s) 228
Cac8I GCNNGC 3 cut(s) 26, 145, 1165
CfoI GCGC 3 cut(s) 143, 592, 1163
Cfr10I RCCGGY 1 cut(s) 143
Cfr13I GGNCC 2 cut(s) 1044, 1174
ClaI ATCGAT 1 cut(s) 878
CseI GACGC 1 cut(s) 263
CsiI ACCWGGT 1 cut(s) 685
Csp6I GTAC 3 cut(s) 261, 690, 751
CspCI CAANNNNNGTGG 2 cut(s) 268, 303
CviAII CATG 4 cut(s) 209, 504, 807, 912
CviQI GTAC 3 cut(s) 261, 690, 751
DdeI CTNAG 6 cut(s) 137, 498, 858, 918, 931, 1048
DpnI GATC 5 cut(s) 11, 337, 573, 586, 871
DpnII GATC 5 cut(s) 9, 335, 571, 584, 869
Eam1104I CTCTTC 2 cut(s) 154, 656
EarI CTCTTC 2 cut(s) 154, 656
EciI GGCGGA 2 cut(s) 55, 145
Ecl136II GAGCTC 1 cut(s) 521
Eco147I AGGCCT 1 cut(s) 542
Eco24I GRGCYC 1 cut(s) 523
Eco32I GATATC 1 cut(s) 1114
Eco47I GGWCC 1 cut(s) 1174
Eco53kI GAGCTC 1 cut(s) 521
Eco88I CYCGRG 2 cut(s) 93, 944
EcoICRI GAGCTC 1 cut(s) 521
EcoO109I RGGNCCY 1 cut(s) 1174
EcoRII CCWGG 1 cut(s) 685
EcoRV GATATC 1 cut(s) 1114
EcoT38I GRGCYC 1 cut(s) 523
FaeI CATG 4 cut(s) 212, 507, 810, 915
FalI AAGNNNNNCTT 5 cut(s) 527, 559, 1133, 1165, 1182
FatI CATG 4 cut(s) 208, 503, 806, 911
FauI CCCGC 1 cut(s) 188
Fnu4HI GCNGC 5 cut(s) 314, 737, 822, 887, 954
FokI GGATG 2 cut(s) 443, 823
FriOI GRGCYC 1 cut(s) 523
Fsp4HI GCNGC 5 cut(s) 314, 737, 822, 887, 954
FspBI CTAG 2 cut(s) 576, 1005
FspI TGCGCA 1 cut(s) 591
GlaI GCGC 3 cut(s) 142, 591, 1162
GluI GCNGC 5 cut(s) 314, 737, 822, 887, 954
GsuI CTGGAG 2 cut(s) 16, 795
HaeII RGCGCY 1 cut(s) 144
HaeIII GGCC 2 cut(s) 542, 1045
HapII CCGG 2 cut(s) 144, 171
HgaI GACGC 1 cut(s) 263
HhaI GCGC 3 cut(s) 143, 592, 1163
Hin1II CATG 4 cut(s) 212, 507, 810, 915
Hin6I GCGC 3 cut(s) 141, 590, 1161
HinP1I GCGC 3 cut(s) 141, 590, 1161
HindIII AAGCTT 1 cut(s) 401
HinfI GANTC 4 cut(s) 96, 803, 842, 875
HpaII CCGG 2 cut(s) 144, 171
Hpy166II GTNNAC 1 cut(s) 905
Hpy188I TCNGA 6 cut(s) 253, 431, 466, 584, 874, 981
Hpy188III TCNNGA 2 cut(s) 33, 524
Hpy8I GTNNAC 1 cut(s) 905
Hpy99I CGWCG 1 cut(s) 104
HpyAV CCTTC 4 cut(s) 263, 286, 532, 1184
HpyCH4III ACNGT 2 cut(s) 444, 603
HpyCH4IV ACGT 4 cut(s) 215, 230, 558, 787
HpyCH4V TGCA 9 cut(s) 4, 212, 221, 316, 381, 421, 477, 682, 899
HpyF10VI GCNNNNNNNGC 3 cut(s) 180, 218, 483
HpyF3I CTNAG 6 cut(s) 137, 498, 858, 918, 931, 1048
HpySE526I ACGT 4 cut(s) 215, 230, 558, 787
Hsp92II CATG 4 cut(s) 212, 507, 810, 915
HspAI GCGC 3 cut(s) 141, 590, 1161
KroI GCCGGC 1 cut(s) 143
KroNI GCCGGC 1 cut(s) 145
Kzo9I GATC 5 cut(s) 9, 335, 571, 584, 869
LguI GCTCTTC 1 cut(s) 154
LmnI GCTCC 3 cut(s) 112, 295, 518
LpnPI CCDG 9 cut(s) 10, 46, 157, 184, 672, 699, 759, 1002, 1079
Lsp1109I GCAGC 5 cut(s) 300, 748, 808, 898, 940
LweI GCATC 4 cut(s) 79, 465, 588, 616
MabI ACCWGGT 1 cut(s) 685
MaeI CTAG 2 cut(s) 576, 1005
MaeII ACGT 4 cut(s) 215, 230, 558, 787
MaeIII GTNAC 2 cut(s) 493, 716
MalI GATC 5 cut(s) 11, 337, 573, 586, 871
MboI GATC 5 cut(s) 9, 335, 571, 584, 869
MboII GAAGA 8 cut(s) 141, 240, 401, 404, 443, 444, 673, 1081
MflI RGATCY 3 cut(s) 571, 584, 869
MhlI GDGCHC 2 cut(s) 241, 523
MluCI AATT 5 cut(s) 74, 376, 472, 730, 969
MlyI GAGTC 1 cut(s) 105
MmeI TCCRAC 2 cut(s) 39, 489
MroNI GCCGGC 1 cut(s) 143
MroXI GAANNNNTTC 1 cut(s) 1194
MseI TTAA 7 cut(s) 324, 399, 471, 866, 1035, 1103, 1139
MspA1I CMGCKG 2 cut(s) 183, 739
MspI CCGG 2 cut(s) 144, 171
MspR9I CCNGG 1 cut(s) 687
MvaI CCWGG 1 cut(s) 687
MwoI GCNNNNNNNGC 3 cut(s) 180, 218, 483
NaeI GCCGGC 1 cut(s) 145
NdeII GATC 5 cut(s) 9, 335, 571, 584, 869
NgoMIV GCCGGC 1 cut(s) 143
NlaIII CATG 4 cut(s) 212, 507, 810, 915
NlaIV GGNNCC 4 cut(s) 134, 277, 573, 1064
NmeAIII GCCGAG 1 cut(s) 512
NsbI TGCGCA 1 cut(s) 591
NspI RCATGY 2 cut(s) 212, 507
PceI AGGCCT 1 cut(s) 542
PciSI GCTCTTC 1 cut(s) 154
PdiI GCCGGC 1 cut(s) 145
PdmI GAANNNNTTC 1 cut(s) 1194
PfeI GAWTC 3 cut(s) 803, 842, 875
PkrI GCNGC 5 cut(s) 315, 738, 823, 888, 955
PleI GAGTC 1 cut(s) 104
PpsI GAGTC 1 cut(s) 104
PpuMI RGGWCCY 1 cut(s) 1174
Psp124BI GAGCTC 1 cut(s) 523
Psp5II RGGWCCY 1 cut(s) 1174
Psp6I CCWGG 1 cut(s) 685
PspGI CCWGG 1 cut(s) 685
PspN4I GGNNCC 4 cut(s) 134, 277, 573, 1064
PspPI GGNCC 2 cut(s) 1044, 1174
PspPPI RGGWCCY 1 cut(s) 1174
PstI CTGCAG 1 cut(s) 318
PsuI RGATCY 3 cut(s) 571, 584, 869
PvuII CAGCTG 1 cut(s) 739
RsaI GTAC 3 cut(s) 262, 691, 752
RsaNI GTAC 3 cut(s) 261, 690, 751
SacI GAGCTC 1 cut(s) 523
SapI GCTCTTC 1 cut(s) 154
SaqAI TTAA 7 cut(s) 324, 399, 471, 866, 1035, 1103, 1139
SatI GCNGC 5 cut(s) 314, 737, 822, 887, 954
Sau3AI GATC 5 cut(s) 9, 335, 571, 584, 869
Sau96I GGNCC 2 cut(s) 1044, 1174
ScaI AGTACT 1 cut(s) 752
SchI GAGTC 1 cut(s) 105
ScrFI CCNGG 1 cut(s) 687
SduI GDGCHC 2 cut(s) 241, 523
SexAI ACCWGGT 1 cut(s) 685
SfaNI GCATC 4 cut(s) 79, 465, 588, 616
SfcI CTRYAG 1 cut(s) 314
SinI GGWCC 1 cut(s) 1174
Sse9I AATT 5 cut(s) 74, 376, 472, 730, 969
SseBI AGGCCT 1 cut(s) 542
SsiI CCGC 3 cut(s) 66, 130, 181
SspMI CTAG 2 cut(s) 576, 1005
SstI GAGCTC 1 cut(s) 523
StuI AGGCCT 1 cut(s) 542
StyD4I CCNGG 1 cut(s) 685
TaaI ACNGT 2 cut(s) 444, 603
TaiI ACGT 4 cut(s) 218, 233, 561, 790
TaqI TCGA 4 cut(s) 102, 267, 878, 1017
TasI AATT 5 cut(s) 74, 376, 472, 730, 969
TatI WGTACW 1 cut(s) 750
TfiI GAWTC 3 cut(s) 803, 842, 875
Tru1I TTAA 7 cut(s) 324, 399, 471, 866, 1035, 1103, 1139
Tru9I TTAA 7 cut(s) 324, 399, 471, 866, 1035, 1103, 1139
TscAI CASTG 1 cut(s) 228
TseI GCWGC 5 cut(s) 313, 736, 821, 886, 953
TspDTI ATGAA 4 cut(s) 665, 795, 896, 900
TspRI CASTG 1 cut(s) 228
VpaK11BI GGWCC 1 cut(s) 1174
XapI RAATTY 1 cut(s) 969
XceI RCATGY 2 cut(s) 212, 507
XmnI GAANNNNTTC 1 cut(s) 1194
XspI CTAG 2 cut(s) 576, 1005
ZrmI AGTACT 1 cut(s) 752
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.