pycom16g14840

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0008608
Physical Location & Seq
Forward (+)
10874826 .. 10876029
1204 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 501 bp
ATGTCCCCTCTCCTATTGAAAGTGCCAAGTTTTAAAACTCAAGAGAGTACCCAAAACGCAGGCCCATCTCCGAAGTTATCCAGTCCAAACTCGAAAAGACGGAGAAACTCGTCACTCTCGCGGTCGGAGAGTCTCAGCCTCCCAGAGCTCCGTCTACTGTTCAGACTTCAGCCATATAAATCTCGAGCTCTCGTTCTTACCTTGAAGTTTCTGGATTGGTATTTGAAGATCGCATTCGGGTCGGCTCTGATTGGAGCCTCCATGGAGTTTTTCATGATCAAGACCGGCTTCTGTAACCAATTACCAGTTGTGCTGGTTAATCCCAAATGGTTTCTGGGATTGAAAGATTTGGAGTTTGATCAACTAAATATTTCTTTTGATTTGTCTTGCTCCGCATTTGAGCCGTCATTGGGGTGCCCTTTGAGTTTGACTATGGTTGGGCCATCTAAGTTTTTCCTTTGCTCCCATCGCCCTCTAACCTCGGCTCTCCACTACTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

167

Amino Acids

18.61

Weight (kDa)

9.69

Isoelectric Point (pI)

60.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 414
AccI GTMKAC 1 cut(s) 154
AccII CGCG 1 cut(s) 121
AciI CCGC 2 cut(s) 121, 393
AcuI CTGAAG 1 cut(s) 152
AfaI GTAC 1 cut(s) 49
AfiI CCNNNNNNNGG 1 cut(s) 410
AgsI TTSAA 4 cut(s) 19, 205, 226, 343
AluBI AGCT 2 cut(s) 148, 188
AluI AGCT 2 cut(s) 148, 188
Alw21I GWGCWC 2 cut(s) 150, 190
Alw26I GTCTC 1 cut(s) 137
Ama87I CYCGRG 1 cut(s) 183
AoxI GGCC 2 cut(s) 61, 440
AspS9I GGNCC 2 cut(s) 62, 440
AvaI CYCGRG 1 cut(s) 183
BaeGI GKGCMC 1 cut(s) 419
BanI GGYRCC 1 cut(s) 414
BanII GRGCYC 2 cut(s) 150, 190
Bbv12I GWGCWC 2 cut(s) 150, 190
BccI CCATC 3 cut(s) 73, 451, 474
BceAI ACGGC 1 cut(s) 388
BcgI CGANNNNNNTGC 2 cut(s) 222, 256
BclI TGATCA 2 cut(s) 276, 358
BcoDI GTCTC 1 cut(s) 137
BmeT110I CYCGRG 1 cut(s) 183
BmgT120I GGNCC 2 cut(s) 62, 440
BmiI GGNNCC 2 cut(s) 256, 416
BpuEI CTTGAG 1 cut(s) 24
BsaJI CCNNGG 2 cut(s) 261, 480
Bsc4I CCNNNNNNNGG 1 cut(s) 410
Bse118I RCCGGY 1 cut(s) 284
Bse1I ACTGG 2 cut(s) 81, 305
BseDI CCNNGG 2 cut(s) 261, 480
BseLI CCNNNNNNNGG 1 cut(s) 410
BseMII CTCAG 1 cut(s) 148
BseNI ACTGG 2 cut(s) 81, 305
BseSI GKGCMC 1 cut(s) 419
Bsh1236I CGCG 1 cut(s) 121
Bsh1285I CGRYCG 1 cut(s) 125
BshFI GGCC 2 cut(s) 63, 442
BshNI GGYRCC 1 cut(s) 414
BsiEI CGRYCG 1 cut(s) 125
BsiHKAI GWGCWC 2 cut(s) 150, 190
BsiHKCI CYCGRG 1 cut(s) 183
BsiSI CCGG 1 cut(s) 285
BslI CCNNNNNNNGG 1 cut(s) 410
BsmAI GTCTC 1 cut(s) 137
BsmI GAATGC 1 cut(s) 233
BsnI GGCC 2 cut(s) 63, 442
BsoBI CYCGRG 1 cut(s) 183
Bsp1286I GDGCHC 3 cut(s) 150, 190, 419
Bsp143I GATC 3 cut(s) 228, 276, 358
Bsp19I CCATGG 1 cut(s) 261
BspACI CCGC 2 cut(s) 121, 393
BspANI GGCC 2 cut(s) 63, 442
BspCNI CTCAG 1 cut(s) 147
BspFNI CGCG 1 cut(s) 121
BspHI TCATGA 1 cut(s) 273
BspLI GGNNCC 2 cut(s) 256, 416
BspT107I GGYRCC 1 cut(s) 414
BsrFI RCCGGY 1 cut(s) 284
BsrI ACTGG 2 cut(s) 81, 305
BssAI RCCGGY 1 cut(s) 284
BssECI CCNNGG 2 cut(s) 261, 480
BssMI GATC 3 cut(s) 228, 276, 358
BssT1I CCWWGG 1 cut(s) 261
Bst4CI ACNGT 1 cut(s) 159
BstC8I GCNNGC 1 cut(s) 61
BstDEI CTNAG 3 cut(s) 134, 447, 498
BstDSI CCRYGG 1 cut(s) 261
BstFNI CGCG 1 cut(s) 121
BstKTI GATC 3 cut(s) 231, 279, 361
BstMAI GTCTC 1 cut(s) 137
BstMBI GATC 3 cut(s) 228, 276, 358
BstMCI CGRYCG 1 cut(s) 125
BstMWI GCNNNNNNNGC 1 cut(s) 468
BstSLI GKGCMC 1 cut(s) 419
BstUI CGCG 1 cut(s) 121
BsuRI GGCC 2 cut(s) 63, 442
BtgI CCRYGG 1 cut(s) 261
BtgZI GCGATG 1 cut(s) 452
Cac8I GCNNGC 1 cut(s) 61
CciI TCATGA 1 cut(s) 273
Cfr10I RCCGGY 1 cut(s) 284
Cfr13I GGNCC 2 cut(s) 62, 440
Csp6I GTAC 1 cut(s) 48
CviAII CATG 2 cut(s) 262, 274
CviQI GTAC 1 cut(s) 48
DdeI CTNAG 3 cut(s) 134, 447, 498
DpnI GATC 3 cut(s) 230, 278, 360
DpnII GATC 3 cut(s) 228, 276, 358
DraI TTTAAA 1 cut(s) 34
Ecl136II GAGCTC 2 cut(s) 148, 188
Eco130I CCWWGG 1 cut(s) 261
Eco24I GRGCYC 2 cut(s) 150, 190
Eco53kI GAGCTC 2 cut(s) 148, 188
Eco57I CTGAAG 1 cut(s) 152
Eco88I CYCGRG 1 cut(s) 183
EcoICRI GAGCTC 2 cut(s) 148, 188
EcoT14I CCWWGG 1 cut(s) 261
EcoT38I GRGCYC 2 cut(s) 150, 190
ErhI CCWWGG 1 cut(s) 261
FaeI CATG 2 cut(s) 265, 277
FaiI YATR 5 cut(s) 175, 177, 263, 275, 434
FalI AAGNNNNNCTT 2 cut(s) 272, 304
FatI CATG 2 cut(s) 261, 273
FbaI TGATCA 2 cut(s) 276, 358
FblI GTMKAC 1 cut(s) 154
FriOI GRGCYC 2 cut(s) 150, 190
HaeIII GGCC 2 cut(s) 63, 442
HapII CCGG 1 cut(s) 285
Hin1II CATG 2 cut(s) 265, 277
HinfI GANTC 1 cut(s) 130
HpaII CCGG 1 cut(s) 285
Hpy166II GTNNAC 1 cut(s) 155
Hpy188I TCNGA 4 cut(s) 72, 127, 164, 249
Hpy188III TCNNGA 5 cut(s) 41, 183, 212, 274, 280
Hpy8I GTNNAC 1 cut(s) 155
HpyCH4III ACNGT 1 cut(s) 159
HpyF10VI GCNNNNNNNGC 1 cut(s) 468
HpyF3I CTNAG 3 cut(s) 134, 447, 498
Hsp92II CATG 2 cut(s) 265, 277
Ksp22I TGATCA 2 cut(s) 276, 358
Kzo9I GATC 3 cut(s) 228, 276, 358
LmnI GCTCC 4 cut(s) 153, 254, 395, 467
LpnPI CCDG 8 cut(s) 45, 94, 156, 197, 298, 299, 318, 320
MaeIII GTNAC 2 cut(s) 111, 293
MalI GATC 3 cut(s) 230, 278, 360
MboI GATC 3 cut(s) 228, 276, 358
MboII GAAGA 1 cut(s) 238
MhlI GDGCHC 3 cut(s) 150, 190, 419
MluCI AATT 1 cut(s) 299
MlyI GAGTC 1 cut(s) 139
MmeI TCCRAC 1 cut(s) 105
MnlI CCTC 5 cut(s) 18, 149, 268, 483, 490
MseI TTAA 2 cut(s) 33, 318
MslI CAYNNNNRTG 1 cut(s) 412
MspI CCGG 1 cut(s) 285
Mva1269I GAATGC 1 cut(s) 233
MvnI CGCG 1 cut(s) 121
MwoI GCNNNNNNNGC 1 cut(s) 468
NcoI CCATGG 1 cut(s) 261
NdeII GATC 3 cut(s) 228, 276, 358
NlaIII CATG 2 cut(s) 265, 277
NlaIV GGNNCC 2 cut(s) 256, 416
NmeAIII GCCGAG 1 cut(s) 461
NmuCI GTSAC 1 cut(s) 111
PaeR7I CTCGAG 1 cut(s) 183
PagI TCATGA 1 cut(s) 273
PctI GAATGC 1 cut(s) 233
PleI GAGTC 1 cut(s) 138
PpsI GAGTC 1 cut(s) 138
Psp124BI GAGCTC 2 cut(s) 150, 190
PspN4I GGNNCC 2 cut(s) 256, 416
PspPI GGNCC 2 cut(s) 62, 440
RsaI GTAC 1 cut(s) 49
RsaNI GTAC 1 cut(s) 48
RseI CAYNNNNRTG 1 cut(s) 412
SacI GAGCTC 2 cut(s) 150, 190
SaqAI TTAA 2 cut(s) 33, 318
Sau3AI GATC 3 cut(s) 228, 276, 358
Sau96I GGNCC 2 cut(s) 62, 440
SchI GAGTC 1 cut(s) 139
SduI GDGCHC 3 cut(s) 150, 190, 419
SetI ASST 4 cut(s) 150, 190, 203, 482
Sfr274I CTCGAG 1 cut(s) 183
SlaI CTCGAG 1 cut(s) 183
SmiMI CAYNNNNRTG 1 cut(s) 412
SmlI CTYRAG 2 cut(s) 39, 183
SmoI CTYRAG 2 cut(s) 39, 183
Sse9I AATT 1 cut(s) 299
SsiI CCGC 2 cut(s) 121, 393
SspI AATATT 1 cut(s) 370
SstI GAGCTC 2 cut(s) 150, 190
StyI CCWWGG 1 cut(s) 261
TaaI ACNGT 1 cut(s) 159
TaqI TCGA 2 cut(s) 92, 184
TasI AATT 1 cut(s) 299
Tru1I TTAA 2 cut(s) 33, 318
Tru9I TTAA 2 cut(s) 33, 318
TseFI GTSAC 1 cut(s) 111
Tsp45I GTSAC 1 cut(s) 111
TspDTI ATGAA 1 cut(s) 262
TspGWI ACGGA 2 cut(s) 115, 140
XcmI CCANNNNNNNNNTGG 1 cut(s) 331
XhoI CTCGAG 1 cut(s) 183
XmiI GTMKAC 1 cut(s) 154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.