Rorug05G0255300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0008608
Physical Location & Seq
Forward (+)
28429513 .. 28432467
2955 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0255300.1

Sequence Viewer

Length: 1797 bp
ATGGGCATTCTTTCATTCATTTTCATTGGAGTTTACTTGTCCCTGTTTTTGTTAAAAATCTCAACTTTAGCTGATTCCATCCCCAACCTTGGCGGCTATGAATTGGGTTTCTCGGGTAGACCAAAGGGTGTTCACAGGAGGAGTCTCTTGGCCTCAACTTCTGGAGACTACTGTGACAACAATGACCTCTGTGGCCCCTATTCAATCTGTATCATCAGTAGTTCACAGGTTTGCAATTGTTTGAAAGGGTTTAAGCCTAAAGCACCAGACAAATTTAACAATGGTGAATATTCAGGTGGTTGTGACCGCACTGAAGCTTTGAATTGCCAAAATAAGGATGATGGGTTTGTGAAATATGCTGGGGTGAAGTTGCCAGATGCTACAGATTCTCGGGTTAACCAGAGTATGAGTCTCGAGGAATGCAGGGAAAATTGCTTCAACAACTGTTCTTGTGTGGCTTATGCAAGCTCTAATGTCAATGGCTGCACTATCTGGTTTGGTGTTCTAAACAACATTAGGAAGCTTTCGGATGGTGGGGAGGATCTGAACATTCGAATACCTGCTTCAGAATTAAAGGGAAACCACTCACCTAAGACAAAGATAGCTGTGATCTTTGCATCTGTTGTTGCGGTTGTAATTGGGCTGCTCTTGTTTGCTTATGGCATTCACAGGAGGAGAATAAAGTTCAAAGAGAAAATGCGAAAGAATGGAATGACTCATCAAAACCATGACGGACAGAATGAAGACTTAGAGCTGCCAATATTTAGTTTGTCCACAATAGTCACTGCCACTGATAACTTTTCATTCAACAAGAAACTTGGAGAAGGTGGCTTTGGCCCTGTATACAAGGGTTGTCTAGTGGATGGGCAAGAAATTGCTGTGAAGAGACTTTCACGAAGTTCAGGGCAAGGACCAACCGAGTTCAAAAATGAAGTACTACTAATAGCCAAACTTCAGCACCGGAATCTTGTAAGGCTCCTAGGTTGTTGCATTGAGGGAGAAGAGAGATTGTTGATCTATGAATTCATGCCCAACAACAGCTTGGACTTCTACCTTTTTGATGAAAATCGAGCAAGACTATTGGCTTGGCCTCAACGCTTTCACATTATCTGTGGAATAGCCAGAGGTCTTCTTTATCTACATCAAGACTCCAGATTGCGGATTATTCATAGAGATCTTAAAGCAAGTAATGTTTTGCTTGATAAGGAGATGAACCCAAAAATCTCAGACTTCGGCATGGCTAGAACTTTTGGAGGAGATCAGACTGAAGGAGTTACAAGAAGAGTTGTTGGAACCTATGGTTATATGGCACCGGAATATGCAATTGATGGTCAATTCTCTGTAAAATCCGACGTTTTTAGTTTTGGCATTTTATTGTTGGAAACATTAAGCGGGAAGAGAAGTAGAGGATTTTATGATCCTGTTGATAACCTTAACCTCATTGGTCATGCATGGCGATTGTGGAAAGAAGGAAGATCTTCGGAGTTGATTGATGGATGCTTAAGGGACTCCTGCAGTCTGTCAGAAATCTTGTGTTGCTTCCATATTAGTCTTTTATGTGTGCAAGAGCTTCCTGAGGACAGGCCAAATATTTCAACCGTGATTCTCATGTTAGGTGGTGGTTCTGCCTTGCCTCTGCCCAAAAAACCTGGTTTTTTTGGTAGAAGTTCATCTGCAGCAGATTCTTCTTCATGTAAGAATGCAACAACATCTTCAACTAACGATGAAACATCTTCAAGTATGAATTATACATATTCAAACTATGACTCTACAATAACAGTATTGGAGGGTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

598

Amino Acids

66.1

Weight (kDa)

7.95

Isoelectric Point (pI)

47.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S_locus_glycop PF00954 52 - 87 2.9e-08 S-locus glycoprotein domain
PAN_2 PF08276 109 - 171 8.5e-18 PAN-like domain
PK_Tyr_Ser-Thr PF07714 268 - 534 8.5e-47 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 268 - 467 2.3e-42 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 568
AccB1I GGYRCC 1 cut(s) 1309
AccI GTMKAC 2 cut(s) 118, 843
AciI CCGC 5 cut(s) 93, 307, 629, 1159, 1392
AclWI GGATC 2 cut(s) 549, 1412
AcsI RAATTY 2 cut(s) 272, 1022
AcuI CTGAAG 4 cut(s) 333, 549, 938, 1287
AfaI GTAC 1 cut(s) 936
AfiI CCNNNNNNNGG 3 cut(s) 89, 334, 1158
AflII CTTAAG 1 cut(s) 1501
AjnI CCWGG 1 cut(s) 1648
AjuI GAANNNNNNNTTGG 2 cut(s) 816, 848
AluBI AGCT 8 cut(s) 71, 317, 468, 523, 605, 754, 1041, 1570
AluI AGCT 8 cut(s) 71, 317, 468, 523, 605, 754, 1041, 1570
Alw26I GTCTC 4 cut(s) 149, 159, 416, 880
AlwI GGATC 2 cut(s) 549, 1412
Ama87I CYCGRG 3 cut(s) 112, 390, 413
AoxI GGCC 5 cut(s) 150, 193, 835, 1088, 1583
ApeKI GCWGC 4 cut(s) 483, 643, 754, 1676
ApoI RAATTY 2 cut(s) 272, 1022
Asp700I GAANNNNTTC 1 cut(s) 1477
AspA2I CCTAGG 1 cut(s) 979
AspS9I GGNCC 3 cut(s) 194, 836, 911
AsuHPI GGTGA 3 cut(s) 296, 376, 579
AsuII TTCGAA 1 cut(s) 553
AvaI CYCGRG 3 cut(s) 112, 390, 413
AvaII GGWCC 1 cut(s) 911
AvrII CCTAGG 1 cut(s) 979
AxyI CCTNAGG 1 cut(s) 1575
BanI GGYRCC 1 cut(s) 1309
BbsI GAAGAC 2 cut(s) 750, 1121
BbvI GCAGC 4 cut(s) 470, 630, 741, 1688
BccI CCATC 6 cut(s) 86, 335, 524, 857, 1322, 1487
BciT130I CCWGG 1 cut(s) 1650
BcoDI GTCTC 4 cut(s) 149, 159, 416, 880
BfaI CTAG 3 cut(s) 857, 980, 1242
BfmI CTRYAG 3 cut(s) 381, 1513, 1674
BfrI CTTAAG 1 cut(s) 1501
BfuAI ACCTGC 1 cut(s) 568
BglII AGATCT 2 cut(s) 1174, 1475
BisI GCNGC 5 cut(s) 94, 484, 644, 755, 1677
BlnI CCTAGG 1 cut(s) 979
BlsI GCNGC 5 cut(s) 95, 485, 645, 756, 1678
BmcAI AGTACT 1 cut(s) 936
Bme1390I CCNGG 1 cut(s) 1650
Bme18I GGWCC 1 cut(s) 911
BmeT110I CYCGRG 3 cut(s) 112, 390, 413
BmgT120I GGNCC 3 cut(s) 194, 836, 911
BmiI GGNNCC 4 cut(s) 196, 977, 1294, 1311
BmrFI CCNGG 1 cut(s) 1650
BmsI GCATC 3 cut(s) 367, 626, 1487
BpiI GAAGAC 2 cut(s) 750, 1121
BpmI CTGGAG 2 cut(s) 183, 1135
Bpu14I TTCGAA 1 cut(s) 553
BsaBI GATNNNNATC 1 cut(s) 1065
BsaJI CCNNGG 2 cut(s) 88, 979
BsaWI WCCGGW 2 cut(s) 960, 1312
Bsc4I CCNNNNNNNGG 3 cut(s) 89, 334, 1158
Bse21I CCTNAGG 1 cut(s) 1575
Bse8I GATNNNNATC 1 cut(s) 1065
BseBI CCWGG 1 cut(s) 1650
BseDI CCNNGG 2 cut(s) 88, 979
BseGI GGATG 5 cut(s) 78, 343, 535, 868, 1502
BseJI GATNNNNATC 1 cut(s) 1065
BseLI CCNNNNNNNGG 3 cut(s) 89, 334, 1158
BseMII CTCAG 2 cut(s) 1239, 1566
BseRI GAGGAG 3 cut(s) 154, 688, 1269
BseXI GCAGC 4 cut(s) 470, 630, 741, 1688
BseYI CCCAGC 1 cut(s) 359
BsgI GTGCAG 1 cut(s) 469
BshFI GGCC 5 cut(s) 152, 195, 837, 1090, 1585
BshNI GGYRCC 1 cut(s) 1309
BsiHKCI CYCGRG 3 cut(s) 112, 390, 413
BsiSI CCGG 2 cut(s) 961, 1313
BslFI GGGAC 2 cut(s) 25, 1520
BslI CCNNNNNNNGG 3 cut(s) 89, 334, 1158
BsmAI GTCTC 4 cut(s) 149, 159, 416, 880
BsmFI GGGAC 2 cut(s) 25, 1520
BsmI GAATGC 4 cut(s) 6, 425, 663, 1705
BsnI GGCC 5 cut(s) 152, 195, 837, 1090, 1585
BsoBI CYCGRG 3 cut(s) 112, 390, 413
Bsp119I TTCGAA 1 cut(s) 553
Bsp143I GATC 7 cut(s) 541, 609, 1014, 1174, 1258, 1417, 1475
BspACI CCGC 5 cut(s) 93, 307, 629, 1159, 1392
BspANI GGCC 5 cut(s) 152, 195, 837, 1090, 1585
BspCNI CTCAG 2 cut(s) 1238, 1567
BspLI GGNNCC 4 cut(s) 196, 977, 1294, 1311
BspMAI CTGCAG 2 cut(s) 1517, 1678
BspMI ACCTGC 1 cut(s) 568
BspPI GGATC 2 cut(s) 549, 1412
BspT104I TTCGAA 1 cut(s) 553
BspT107I GGYRCC 1 cut(s) 1309
BspTI CTTAAG 1 cut(s) 1501
BssECI CCNNGG 2 cut(s) 88, 979
BssMI GATC 7 cut(s) 541, 609, 1014, 1174, 1258, 1417, 1475
BssNAI GTATAC 1 cut(s) 844
BssT1I CCWWGG 2 cut(s) 88, 979
Bst1107I GTATAC 1 cut(s) 844
Bst2UI CCWGG 1 cut(s) 1650
Bst4CI ACNGT 4 cut(s) 173, 446, 1600, 1780
Bst6I CTCTTC 4 cut(s) 878, 996, 1276, 1391
BstAFI CTTAAG 1 cut(s) 1501
BstBI TTCGAA 1 cut(s) 553
BstC8I GCNNGC 1 cut(s) 466
BstDEI CTNAG 4 cut(s) 591, 748, 1225, 1575
BstF5I GGATG 5 cut(s) 78, 343, 535, 868, 1502
BstKTI GATC 7 cut(s) 544, 612, 1017, 1177, 1261, 1420, 1478
BstMAI GTCTC 4 cut(s) 149, 159, 416, 880
BstMBI GATC 7 cut(s) 541, 609, 1014, 1174, 1258, 1417, 1475
BstNI CCWGG 1 cut(s) 1650
BstSCI CCNGG 1 cut(s) 1648
BstSFI CTRYAG 3 cut(s) 381, 1513, 1674
BstV1I GCAGC 4 cut(s) 470, 630, 741, 1688
BstV2I GAAGAC 2 cut(s) 750, 1121
BstX2I RGATCY 3 cut(s) 541, 1174, 1475
BstYI RGATCY 3 cut(s) 541, 1174, 1475
BstZ17I GTATAC 1 cut(s) 844
Bsu36I CCTNAGG 1 cut(s) 1575
BsuRI GGCC 5 cut(s) 152, 195, 837, 1090, 1585
BtsCI GGATG 5 cut(s) 78, 343, 535, 868, 1502
BtsI GCAGTG 1 cut(s) 783
BtsIMutI CAGTG 3 cut(s) 309, 783, 789
BveI ACCTGC 1 cut(s) 568
Cac8I GCNNGC 1 cut(s) 466
Cfr13I GGNCC 3 cut(s) 194, 836, 911
CsiI ACCWGGT 1 cut(s) 1648
Csp6I GTAC 1 cut(s) 935
CviAII CATG 7 cut(s) 728, 1027, 1237, 1448, 1452, 1609, 1692
CviQI GTAC 1 cut(s) 935
DdeI CTNAG 4 cut(s) 591, 748, 1225, 1575
DpnI GATC 7 cut(s) 543, 611, 1016, 1176, 1260, 1419, 1477
DpnII GATC 7 cut(s) 541, 609, 1014, 1174, 1258, 1417, 1475
Eam1104I CTCTTC 4 cut(s) 878, 996, 1276, 1391
EarI CTCTTC 4 cut(s) 878, 996, 1276, 1391
Eco130I CCWWGG 2 cut(s) 88, 979
Eco47I GGWCC 1 cut(s) 911
Eco57I CTGAAG 4 cut(s) 333, 549, 938, 1287
Eco81I CCTNAGG 1 cut(s) 1575
Eco88I CYCGRG 3 cut(s) 112, 390, 413
EcoRI GAATTC 1 cut(s) 1022
EcoRII CCWGG 1 cut(s) 1648
EcoT14I CCWWGG 2 cut(s) 88, 979
EcoT22I ATGCAT 1 cut(s) 1453
ErhI CCWWGG 2 cut(s) 88, 979
FaeI CATG 7 cut(s) 731, 1030, 1240, 1451, 1455, 1612, 1695
FaqI GGGAC 2 cut(s) 25, 1520
FatI CATG 7 cut(s) 727, 1026, 1236, 1447, 1451, 1608, 1691
FauI CCCGC 1 cut(s) 1385
FblI GTMKAC 2 cut(s) 118, 843
Fnu4HI GCNGC 5 cut(s) 94, 484, 644, 755, 1677
FokI GGATG 5 cut(s) 65, 350, 542, 875, 1509
Fsp4HI GCNGC 5 cut(s) 94, 484, 644, 755, 1677
FspBI CTAG 3 cut(s) 857, 980, 1242
GluI GCNGC 5 cut(s) 94, 484, 644, 755, 1677
GsaI CCCAGC 1 cut(s) 363
GsuI CTGGAG 2 cut(s) 183, 1135
HaeIII GGCC 5 cut(s) 152, 195, 837, 1090, 1585
HapII CCGG 2 cut(s) 961, 1313
Hin1II CATG 7 cut(s) 731, 1030, 1240, 1451, 1455, 1612, 1695
HincII GTYRAC 1 cut(s) 397
HindII GTYRAC 1 cut(s) 397
HindIII AAGCTT 2 cut(s) 315, 521
HpaI GTTAAC 1 cut(s) 397
HpaII CCGG 2 cut(s) 961, 1313
HphI GGTGA 3 cut(s) 296, 376, 579
Hpy166II GTNNAC 7 cut(s) 34, 119, 133, 224, 397, 774, 844
Hpy188I TCNGA 8 cut(s) 529, 546, 568, 1228, 1263, 1351, 1483, 1525
Hpy188III TCNNGA 6 cut(s) 162, 413, 894, 1145, 1152, 1574
Hpy8I GTNNAC 7 cut(s) 34, 119, 133, 224, 397, 774, 844
Hpy99I CGWCG 1 cut(s) 1355
HpyAV CCTTC 3 cut(s) 818, 1262, 1463
HpyCH4III ACNGT 4 cut(s) 173, 446, 1600, 1780
HpyCH4IV ACGT 1 cut(s) 1353
HpyF3I CTNAG 4 cut(s) 591, 748, 1225, 1575
HpySE526I ACGT 1 cut(s) 1353
Hsp92II CATG 7 cut(s) 731, 1030, 1240, 1451, 1455, 1612, 1695
KspAI GTTAAC 1 cut(s) 397
Kzo9I GATC 7 cut(s) 541, 609, 1014, 1174, 1258, 1417, 1475
LmnI GCTCC 1 cut(s) 981
Lsp1109I GCAGC 4 cut(s) 470, 630, 741, 1688
LweI GCATC 3 cut(s) 367, 626, 1487
MabI ACCWGGT 1 cut(s) 1648
MaeI CTAG 3 cut(s) 857, 980, 1242
MaeII ACGT 1 cut(s) 1353
MaeIII GTNAC 4 cut(s) 173, 302, 781, 1273
MalI GATC 7 cut(s) 543, 611, 1016, 1176, 1260, 1419, 1477
MboI GATC 7 cut(s) 541, 609, 1014, 1174, 1258, 1417, 1475
MfeI CAATTG 2 cut(s) 235, 1323
MflI RGATCY 3 cut(s) 541, 1174, 1475
MlyI GAGTC 6 cut(s) 151, 418, 709, 1142, 1502, 1760
MmeI TCCRAC 3 cut(s) 1270, 1359, 1374
Mph1103I ATGCAT 1 cut(s) 1453
MroXI GAANNNNTTC 1 cut(s) 1477
MseI TTAA 9 cut(s) 53, 252, 276, 396, 572, 1179, 1388, 1434, 1502
MspCI CTTAAG 1 cut(s) 1501
MspI CCGG 2 cut(s) 961, 1313
MspR9I CCNGG 1 cut(s) 1650
MunI CAATTG 2 cut(s) 235, 1323
Mva1269I GAATGC 4 cut(s) 6, 425, 663, 1705
MvaI CCWGG 1 cut(s) 1650
NdeII GATC 7 cut(s) 541, 609, 1014, 1174, 1258, 1417, 1475
NlaIII CATG 7 cut(s) 731, 1030, 1240, 1451, 1455, 1612, 1695
NlaIV GGNNCC 4 cut(s) 196, 977, 1294, 1311
NmuCI GTSAC 3 cut(s) 173, 302, 781
NsiI ATGCAT 1 cut(s) 1453
NspV TTCGAA 1 cut(s) 553
PaeR7I CTCGAG 1 cut(s) 413
PctI GAATGC 4 cut(s) 6, 425, 663, 1705
PdmI GAANNNNTTC 1 cut(s) 1477
PfeI GAWTC 5 cut(s) 74, 386, 964, 1603, 1682
PkrI GCNGC 5 cut(s) 95, 485, 645, 756, 1678
PleI GAGTC 6 cut(s) 150, 417, 709, 1142, 1502, 1760
PpsI GAGTC 6 cut(s) 150, 417, 709, 1142, 1502, 1760
Psp6I CCWGG 1 cut(s) 1648
PspFI CCCAGC 1 cut(s) 359
PspGI CCWGG 1 cut(s) 1648
PspN4I GGNNCC 4 cut(s) 196, 977, 1294, 1311
PspPI GGNCC 3 cut(s) 194, 836, 911
PstI CTGCAG 2 cut(s) 1517, 1678
PsuI RGATCY 3 cut(s) 541, 1174, 1475
RsaI GTAC 1 cut(s) 936
RsaNI GTAC 1 cut(s) 935
SaqAI TTAA 9 cut(s) 53, 252, 276, 396, 572, 1179, 1388, 1434, 1502
SatI GCNGC 5 cut(s) 94, 484, 644, 755, 1677
Sau3AI GATC 7 cut(s) 541, 609, 1014, 1174, 1258, 1417, 1475
Sau96I GGNCC 3 cut(s) 194, 836, 911
ScaI AGTACT 1 cut(s) 936
SchI GAGTC 6 cut(s) 151, 418, 709, 1142, 1502, 1760
ScrFI CCNGG 1 cut(s) 1650
SexAI ACCWGGT 1 cut(s) 1648
SfaNI GCATC 3 cut(s) 367, 626, 1487
SfcI CTRYAG 3 cut(s) 381, 1513, 1674
Sfr274I CTCGAG 1 cut(s) 413
SfuI TTCGAA 1 cut(s) 553
SinI GGWCC 1 cut(s) 911
SlaI CTCGAG 1 cut(s) 413
SmlI CTYRAG 2 cut(s) 413, 1501
SmoI CTYRAG 2 cut(s) 413, 1501
SsiI CCGC 5 cut(s) 93, 307, 629, 1159, 1392
SspI AATATT 3 cut(s) 290, 762, 1591
SspMI CTAG 3 cut(s) 857, 980, 1242
StyD4I CCNGG 1 cut(s) 1648
StyI CCWWGG 2 cut(s) 88, 979
TaaI ACNGT 4 cut(s) 173, 446, 1600, 1780
TaiI ACGT 1 cut(s) 1356
TaqI TCGA 4 cut(s) 414, 553, 1069, 1792
TatI WGTACW 1 cut(s) 934
TauI GCSGC 1 cut(s) 96
TfiI GAWTC 5 cut(s) 74, 386, 964, 1603, 1682
Tru1I TTAA 9 cut(s) 53, 252, 276, 396, 572, 1179, 1388, 1434, 1502
Tru9I TTAA 9 cut(s) 53, 252, 276, 396, 572, 1179, 1388, 1434, 1502
TscAI CASTG 3 cut(s) 316, 790, 796
TseFI GTSAC 3 cut(s) 173, 302, 781
TseI GCWGC 4 cut(s) 483, 643, 754, 1676
Tsp45I GTSAC 3 cut(s) 173, 302, 781
TspGWI ACGGA 1 cut(s) 747
TspRI CASTG 3 cut(s) 316, 790, 796
Vha464I CTTAAG 1 cut(s) 1501
VpaK11BI GGWCC 1 cut(s) 911
XapI RAATTY 2 cut(s) 272, 1022
XcmI CCANNNNNNNNNTGG 1 cut(s) 1039
XhoI CTCGAG 1 cut(s) 413
XmaJI CCTAGG 1 cut(s) 979
XmiI GTMKAC 2 cut(s) 118, 843
XmnI GAANNNNTTC 1 cut(s) 1477
XspI CTAG 3 cut(s) 857, 980, 1242
ZrmI AGTACT 1 cut(s) 936
Zsp2I ATGCAT 1 cut(s) 1453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.