Rorug01G0335000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0008608
Physical Location & Seq
Reverse (-)
45309360 .. 45309755
396 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0335000.1

Sequence Viewer

Length: 396 bp
ATGTCAAATGTTGTGAGATTGCGGAGCTCGAACAACGAAGTGTTCGAAGCAGCCGAGTGCATGATAGCCATGTCCAAAACCATTCAGACAATGCTAAATAGGTTCCAAGACGACCAAGTGGTGATACTTCCACTGGAACAAGTGGATTCCAAAACCCTAGCCATGGTAAGAGAGTGGTGTGACAATCACGTCACGAAGAGCACCATAAAACAGTACCAATTTGTGGAGGGACTCGACATAGACGTTCTCTTGCATCTCCTGATAACTGCAAACTATCTGGACATCAAGGAGTTGGGGAATCATATGCGCGAAGCGATTGCGGAGAGGGCTCAAAGGAAAGCACTTGAAGAGATCGATCCCAGAAGTTTTCATACTGCAGTTTTTTTGAAAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

131

Amino Acids

15.08

Weight (kDa)

5.54

Isoelectric Point (pI)

36.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Skp1_POZ PF03931 4 - 64 2.7e-11 Skp1 family, tetramerisation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 188
AccB7I CCANNNNNTGG 1 cut(s) 223
AccII CGCG 1 cut(s) 309
AciI CCGC 2 cut(s) 22, 320
AclWI GGATC 1 cut(s) 350
AfaI GTAC 1 cut(s) 215
AfiI CCNNNNNNNGG 2 cut(s) 163, 223
AgsI TTSAA 2 cut(s) 347, 388
AjiI CACGTC 1 cut(s) 190
AluBI AGCT 1 cut(s) 27
AluI AGCT 1 cut(s) 27
Alw21I GWGCWC 2 cut(s) 29, 203
AlwI GGATC 1 cut(s) 350
ApeKI GCWGC 1 cut(s) 50
AspLEI GCGC 1 cut(s) 309
AsuHPI GGTGA 1 cut(s) 133
AsuII TTCGAA 1 cut(s) 45
BanII GRGCYC 2 cut(s) 29, 331
BarI GAAGNNNNNNTAC 2 cut(s) 355, 387
Bbv12I GWGCWC 2 cut(s) 29, 203
BbvI GCAGC 1 cut(s) 62
BcgI CGANNNNNNTGC 2 cut(s) 299, 333
BfaI CTAG 1 cut(s) 158
BfmI CTRYAG 1 cut(s) 375
BisI GCNGC 1 cut(s) 51
BlsI GCNGC 1 cut(s) 52
BmgBI CACGTC 1 cut(s) 190
BmiI GGNNCC 1 cut(s) 104
BmsI GCATC 1 cut(s) 262
Bpu14I TTCGAA 1 cut(s) 45
Bsa29I ATCGAT 1 cut(s) 354
BsaJI CCNNGG 1 cut(s) 162
Bsc4I CCNNNNNNNGG 2 cut(s) 163, 223
Bse1I ACTGG 1 cut(s) 138
BseCI ATCGAT 1 cut(s) 354
BseDI CCNNGG 1 cut(s) 162
BseLI CCNNNNNNNGG 2 cut(s) 163, 223
BseNI ACTGG 1 cut(s) 138
BseXI GCAGC 1 cut(s) 62
Bsh1236I CGCG 1 cut(s) 309
BshVI ATCGAT 1 cut(s) 354
BsiHKAI GWGCWC 2 cut(s) 29, 203
BslFI GGGAC 1 cut(s) 243
BslI CCNNNNNNNGG 2 cut(s) 163, 223
BsmFI GGGAC 1 cut(s) 243
Bsp119I TTCGAA 1 cut(s) 45
Bsp1286I GDGCHC 3 cut(s) 29, 203, 331
Bsp143I GATC 2 cut(s) 351, 355
Bsp19I CCATGG 1 cut(s) 162
BspACI CCGC 2 cut(s) 22, 320
BspDI ATCGAT 1 cut(s) 354
BspFNI CGCG 1 cut(s) 309
BspLI GGNNCC 1 cut(s) 104
BspMAI CTGCAG 1 cut(s) 379
BspPI GGATC 1 cut(s) 350
BspQI GCTCTTC 1 cut(s) 191
BspT104I TTCGAA 1 cut(s) 45
BsrI ACTGG 1 cut(s) 138
BssECI CCNNGG 1 cut(s) 162
BssMI GATC 2 cut(s) 351, 355
BssT1I CCWWGG 1 cut(s) 162
Bst4CI ACNGT 1 cut(s) 213
Bst6I CTCTTC 2 cut(s) 191, 342
BstBI TTCGAA 1 cut(s) 45
BstDSI CCRYGG 1 cut(s) 162
BstFNI CGCG 1 cut(s) 309
BstHHI GCGC 1 cut(s) 309
BstKTI GATC 2 cut(s) 354, 358
BstMBI GATC 2 cut(s) 351, 355
BstMWI GCNNNNNNNGC 1 cut(s) 326
BstSFI CTRYAG 1 cut(s) 375
BstUI CGCG 1 cut(s) 309
BstV1I GCAGC 1 cut(s) 62
Bsu15I ATCGAT 1 cut(s) 354
BsuTUI ATCGAT 1 cut(s) 354
BtgI CCRYGG 1 cut(s) 162
BtrI CACGTC 1 cut(s) 190
BtsIMutI CAGTG 1 cut(s) 131
CfoI GCGC 1 cut(s) 309
ClaI ATCGAT 1 cut(s) 354
Csp6I GTAC 1 cut(s) 214
CviAII CATG 3 cut(s) 61, 70, 163
CviJI RGCY 5 cut(s) 27, 53, 68, 161, 329
CviKI_1 RGCY 5 cut(s) 27, 53, 68, 161, 329
CviQI GTAC 1 cut(s) 214
DpnI GATC 2 cut(s) 353, 357
DpnII GATC 2 cut(s) 351, 355
DrdI GACNNNNNNGTC 1 cut(s) 188
DseDI GACNNNNNNGTC 1 cut(s) 188
Eam1104I CTCTTC 2 cut(s) 191, 342
EarI CTCTTC 2 cut(s) 191, 342
Ecl136II GAGCTC 1 cut(s) 27
Eco130I CCWWGG 1 cut(s) 162
Eco24I GRGCYC 2 cut(s) 29, 331
Eco53kI GAGCTC 1 cut(s) 27
EcoICRI GAGCTC 1 cut(s) 27
EcoT14I CCWWGG 1 cut(s) 162
EcoT38I GRGCYC 2 cut(s) 29, 331
ErhI CCWWGG 1 cut(s) 162
FaeI CATG 3 cut(s) 64, 73, 166
FaiI YATR 8 cut(s) 62, 71, 164, 206, 239, 303, 305, 372
FaqI GGGAC 1 cut(s) 243
FatI CATG 3 cut(s) 60, 69, 162
FauNDI CATATG 1 cut(s) 303
Fnu4HI GCNGC 1 cut(s) 51
FriOI GRGCYC 2 cut(s) 29, 331
Fsp4HI GCNGC 1 cut(s) 51
FspBI CTAG 1 cut(s) 158
GlaI GCGC 1 cut(s) 308
GluI GCNGC 1 cut(s) 51
HhaI GCGC 1 cut(s) 309
Hin1II CATG 3 cut(s) 64, 73, 166
Hin6I GCGC 1 cut(s) 307
HinP1I GCGC 1 cut(s) 307
HinfI GANTC 3 cut(s) 146, 231, 298
HphI GGTGA 1 cut(s) 133
Hpy188I TCNGA 1 cut(s) 87
Hpy188III TCNNGA 3 cut(s) 193, 259, 278
HpyCH4III ACNGT 1 cut(s) 213
HpyCH4IV ACGT 2 cut(s) 189, 243
HpyCH4V TGCA 4 cut(s) 60, 253, 269, 377
HpyF10VI GCNNNNNNNGC 1 cut(s) 326
HpySE526I ACGT 2 cut(s) 189, 243
Hsp92II CATG 3 cut(s) 64, 73, 166
HspAI GCGC 1 cut(s) 307
Kzo9I GATC 2 cut(s) 351, 355
LguI GCTCTTC 1 cut(s) 191
LmnI GCTCC 1 cut(s) 24
LpnPI CCDG 4 cut(s) 119, 263, 272, 373
Lsp1109I GCAGC 1 cut(s) 62
LweI GCATC 1 cut(s) 262
MaeI CTAG 1 cut(s) 158
MaeII ACGT 2 cut(s) 189, 243
MaeIII GTNAC 2 cut(s) 179, 190
MalI GATC 2 cut(s) 353, 357
MboI GATC 2 cut(s) 351, 355
MboII GAAGA 2 cut(s) 208, 359
MhlI GDGCHC 3 cut(s) 29, 203, 331
MluCI AATT 1 cut(s) 218
MlyI GAGTC 1 cut(s) 225
MnlI CCTC 2 cut(s) 220, 318
MvnI CGCG 1 cut(s) 309
MwoI GCNNNNNNNGC 1 cut(s) 326
NcoI CCATGG 1 cut(s) 162
NdeI CATATG 1 cut(s) 303
NdeII GATC 2 cut(s) 351, 355
NlaIII CATG 3 cut(s) 64, 73, 166
NlaIV GGNNCC 1 cut(s) 104
NmeAIII GCCGAG 1 cut(s) 79
NmuCI GTSAC 2 cut(s) 179, 190
NspV TTCGAA 1 cut(s) 45
PciSI GCTCTTC 1 cut(s) 191
PcsI WCGNNNNNNNCGW 3 cut(s) 42, 51, 240
PfeI GAWTC 2 cut(s) 146, 298
PflMI CCANNNNNTGG 1 cut(s) 223
PkrI GCNGC 1 cut(s) 52
PleI GAGTC 1 cut(s) 225
PpsI GAGTC 1 cut(s) 225
Psp124BI GAGCTC 1 cut(s) 29
PspN4I GGNNCC 1 cut(s) 104
PstI CTGCAG 1 cut(s) 379
RsaI GTAC 1 cut(s) 215
RsaNI GTAC 1 cut(s) 214
SacI GAGCTC 1 cut(s) 29
SapI GCTCTTC 1 cut(s) 191
SatI GCNGC 1 cut(s) 51
Sau3AI GATC 2 cut(s) 351, 355
SchI GAGTC 1 cut(s) 225
SduI GDGCHC 3 cut(s) 29, 203, 331
SetI ASST 4 cut(s) 29, 104, 192, 246
SfaNI GCATC 1 cut(s) 262
SfcI CTRYAG 1 cut(s) 375
SfuI TTCGAA 1 cut(s) 45
Sse9I AATT 1 cut(s) 218
SsiI CCGC 2 cut(s) 22, 320
SspMI CTAG 1 cut(s) 158
SstI GAGCTC 1 cut(s) 29
StyI CCWWGG 1 cut(s) 162
TaaI ACNGT 1 cut(s) 213
TaiI ACGT 2 cut(s) 192, 246
TaqI TCGA 4 cut(s) 29, 45, 234, 354
TasI AATT 1 cut(s) 218
TfiI GAWTC 2 cut(s) 146, 298
TscAI CASTG 1 cut(s) 138
TseFI GTSAC 2 cut(s) 179, 190
TseI GCWGC 1 cut(s) 50
Tsp45I GTSAC 2 cut(s) 179, 190
TspDTI ATGAA 1 cut(s) 359
TspRI CASTG 1 cut(s) 138
Van91I CCANNNNNTGG 1 cut(s) 223
XspI CTAG 1 cut(s) 158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.