pycom16g17590

Zinc knuckle (CCHC-type) family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0001074
Physical Location & Seq
Reverse (-)
13843629 .. 13846102
2474 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2055 bp
ATGGGAAGTGATTGTTATTTTTCTTGTAGGAACATAGAGCCTGTGACTGATTTGGGACTAGTTCTGGGTCATTCAAATCAATGCATTCAGAGAAAGTTGAATGGTGATTCAGGTGCAGGTGCAAATGCAGGTTCAAGGATACACATGGCATTCGTGGCTACCGATCCCCTTTCAGAATTAGTATGGGATACAGATAAAGGTGTAAGCAATGTCACTCTTTCACCACCACAAAGCAATAAAGGTGGGAGGTCTGCTGCTGACAAGCCTATTGATGACGAGGACTTTATAACAGCACAGACATCATTTCGTCTAAAGAGCGAATTTGCTGGTAAAGATATGACTATGTCTCCCACAAGCAATGCTGGTGTCATGCCATTATGCGGGTCAAGCCATGACACGGGAACTGTTGGTAACGTGGAGGAAGAGAAAGCGGCTGTGGAACTATCTGTTCTGTACAACCAGGAGGGGACTTATAGTCAAGTGAATATCGAAGTAAATGAAATACCTCAGATACCTGAAACCAGAGAAAATTTCATTACAACATTGACAGGTAATGGTGACAGAGAGGGGGCTGATATCTTATTAGTCGAATCAGATCAAAAGATACCTTTTGTGGAACAAAATGAACCATTATTGGGGGATCCTGTTGATGAAGATAAACATGCTGATGTTGGTGATCAGAAAATGCAAATGAATCTTGTTTTGGCTTCCGAAGTTCACCCAGTGAACGAAAGTAAAGCTTCAGGTGTCCCTGTGGTGAATAAAAAGGCACAAAGCAATAGGCCTTTGAATAAACTAGAGGCAACAGCTGAGAATGATTTAATGACTATCAAAATCCGACATGCTTCTGGTGCAGCGAGTCAGATTTTAGGTCCAGAATCTGTCCCAGGGGTTAAGGACAGGTTTGATCAGGGTCAGGAGATGGTTCCTGGAAACGAATCTGTTCTGGACAGACACTATCCTAATAACAGCAGAATCCATATGCATCAAAGGAAAGGAAAAGAAAATTTCTTATCTGATGGAGATCCTAATGGAAGAATGCCAGAAGATGAGACTGATAGCCATGAGAGTGTCGAGAGCTGTAATAGCGCAGGATTGTTTACGTCAGGCAAGAAGAGGTGGAGATCTGAAGAAGAGTTCATTGTTGGGAGTAAAAGATTCAGAAAGAAAATTCAAGAAACTCCGATTTCCACATCCTATATTAGACAAGATAGCTCCTTTATGAATTGGATATCAAGCATGGTGAAGGGGTTTTCAAAATCAATGCAAGATGAGGCACCACCTCTTGCTCTTACCCTTGCACATCCTGATCATGGAGATGAGAATCCTGATAATAAACGTATCACATGTAATACAAACCAAGATGATGGAGTAAAAAGTATCGGTTTCCAGACAATTTTTCAGTCCTTGTATTGCCCAACTGCAGACTGCCAAGAAGCACGGATGTTGAGTGACAATCATAAAAAAGGAGAAATATCAACGGAACTCGAGCCAGCTACTTCTCCAAAAGTCTATTGTGGGGAGAACGTTAATTTAGGCCAAGAATTTCTACTGTCAGTTGAGAAGTTCAACAAATCTTCATCTGGAAATGAAGTCCATTCAGCAGGTAGTCAGGAGAAATGCCATACTGGTTCCGAGAAGACAAAGAATCCATGCAACTTTCCATTTTATAAAAGGAAAGATAGAGTTATCCCTAATTCTTCTCTGGGTAAACACAAGAAAAGGAGGGTCGAGAATGTTGAATCTAGCTCACAATTCAAAGGGAAGACGACTGGTGAATTTGGTTATAGAAGGGACCTTCTGGCAAGCTTGTGGATAACTCGGTTGACTCCAAAAACATCTGGTCCATCTTTAATCACAGATCATTCCAACAAGAGTGCTGATGGAGTTCTTGAGAGCTCCAATGACCTCAAAAATATGGGGGCCAGGGAGCAGTTTGCTGAAGATTTAGTTATTGTCATTGGTAACGATCTGCAAAATTGTGCGGCTGACAACGAGGGCTCATCTGCTTTCAATAGAAACAAAGGCCAGAATCGATCAAGCGCCCATGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Plus-3

Protein Analysis

685

Amino Acids

75.0

Weight (kDa)

5.42

Isoelectric Point (pI)

45.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 287, 1671
AarI CACCTGC 1 cut(s) 107
Acc36I ACCTGC 3 cut(s) 107, 119, 1595
AccB1I GGYRCC 1 cut(s) 1276
AciI CCGC 3 cut(s) 381, 431, 1985
AclI AACGTT 1 cut(s) 1527
AclWI GGATC 4 cut(s) 158, 635, 648, 1019
AcsI RAATTY 6 cut(s) 320, 529, 1006, 1170, 1544, 1778
AcuI CTGAAG 3 cut(s) 726, 1149, 1962
AdeI CACNNNGTG 1 cut(s) 724
AfaI GTAC 1 cut(s) 455
AfiI CCNNNNNNNGG 4 cut(s) 380, 397, 635, 1313
AflIII ACRYGT 1 cut(s) 1346
AhdI GACNNNNNGTC 1 cut(s) 474
AhlI ACTAGT 1 cut(s) 58
AjnI CCWGG 4 cut(s) 459, 886, 928, 1925
AjuI GAANNNNNNNTTGG 2 cut(s) 686, 718
AluBI AGCT 8 cut(s) 740, 809, 1080, 1215, 1496, 1749, 1809, 1899
AluI AGCT 8 cut(s) 740, 809, 1080, 1215, 1496, 1749, 1809, 1899
Alw21I GWGCWC 1 cut(s) 1901
Alw26I GTCTC 2 cut(s) 351, 1046
AlwI GGATC 4 cut(s) 158, 635, 648, 1019
AlwNI CAGNNNCTG 1 cut(s) 881
Ama87I CYCGRG 1 cut(s) 1487
AoxI GGCC 4 cut(s) 782, 1537, 1923, 2026
ApeKI GCWGC 2 cut(s) 254, 854
ApoI RAATTY 6 cut(s) 320, 529, 1006, 1170, 1544, 1778
Asp700I GAANNNNTTC 1 cut(s) 942
AspLEI GCGC 2 cut(s) 1091, 2045
AspS9I GGNCC 4 cut(s) 872, 1795, 1844, 1923
AsuHPI GGTGA 8 cut(s) 116, 213, 569, 686, 710, 769, 1255, 1787
AvaI CYCGRG 1 cut(s) 1487
AvaII GGWCC 3 cut(s) 872, 1795, 1844
BamHI GGATCC 1 cut(s) 640
BanI GGYRCC 1 cut(s) 1276
BanII GRGCYC 2 cut(s) 1901, 2003
BbsI GAAGAC 2 cut(s) 1646, 1772
Bbv12I GWGCWC 1 cut(s) 1901
BbvI GCAGC 2 cut(s) 241, 866
BccI CCATC 5 cut(s) 916, 1013, 1361, 1855, 1877
BciT130I CCWGG 4 cut(s) 461, 888, 930, 1927
BciVI GTATCC 2 cut(s) 132, 181
BclI TGATCA 3 cut(s) 676, 907, 1309
BcoDI GTCTC 2 cut(s) 351, 1046
BcuI ACTAGT 1 cut(s) 58
BfaI CTAG 3 cut(s) 59, 797, 1746
BfmI CTRYAG 1 cut(s) 1422
BfoI RGCGCY 1 cut(s) 2046
BfuAI ACCTGC 3 cut(s) 107, 119, 1595
BfuI GTATCC 2 cut(s) 132, 181
BglII AGATCT 1 cut(s) 1124
BisI GCNGC 4 cut(s) 255, 432, 855, 1986
BlsI GCNGC 4 cut(s) 256, 433, 856, 1987
Bme1390I CCNGG 4 cut(s) 461, 888, 930, 1927
Bme18I GGWCC 3 cut(s) 872, 1795, 1844
BmeRI GACNNNNNGTC 1 cut(s) 474
BmeT110I CYCGRG 1 cut(s) 1487
BmgT120I GGNCC 4 cut(s) 872, 1795, 1844, 1923
BmiI GGNNCC 6 cut(s) 642, 927, 1278, 1633, 1796, 1924
BmrFI CCNGG 4 cut(s) 461, 888, 930, 1927
BmrI ACTGGG 1 cut(s) 716
BmsI GCATC 1 cut(s) 994
BmuI ACTGGG 1 cut(s) 716
BpiI GAAGAC 2 cut(s) 1646, 1772
BpuEI CTTGAG 1 cut(s) 1913
Bsa29I ATCGAT 1 cut(s) 2035
BsaBI GATNNNNATC 2 cut(s) 1023, 1323
BsaJI CCNNGG 3 cut(s) 886, 887, 1926
BsaXI ACNNNNNCTCC 2 cut(s) 331, 361
Bsc4I CCNNNNNNNGG 4 cut(s) 380, 397, 635, 1313
Bse1I ACTGG 3 cut(s) 722, 1633, 1777
Bse3DI GCAATG 2 cut(s) 214, 364
Bse8I GATNNNNATC 2 cut(s) 1023, 1323
BseBI CCWGG 4 cut(s) 461, 888, 930, 1927
BseCI ATCGAT 1 cut(s) 2035
BseDI CCNNGG 3 cut(s) 886, 887, 1926
BseGI GGATG 3 cut(s) 1193, 1303, 1449
BseJI GATNNNNATC 2 cut(s) 1023, 1323
BseLI CCNNNNNNNGG 4 cut(s) 380, 397, 635, 1313
BseMI GCAATG 2 cut(s) 214, 364
BseMII CTCAG 2 cut(s) 521, 801
BseNI ACTGG 3 cut(s) 722, 1633, 1777
BseXI GCAGC 2 cut(s) 241, 866
BsgI GTGCAG 2 cut(s) 135, 873
BshFI GGCC 4 cut(s) 784, 1539, 1925, 2028
BshNI GGYRCC 1 cut(s) 1276
BshVI ATCGAT 1 cut(s) 2035
BsiHKAI GWGCWC 1 cut(s) 1901
BsiHKCI CYCGRG 1 cut(s) 1487
BslFI GGGAC 5 cut(s) 69, 481, 734, 869, 1808
BslI CCNNNNNNNGG 4 cut(s) 380, 397, 635, 1313
BsmAI GTCTC 2 cut(s) 351, 1046
BsmFI GGGAC 5 cut(s) 69, 481, 734, 869, 1808
BsmI GAATGC 3 cut(s) 84, 149, 1044
BsnI GGCC 4 cut(s) 784, 1539, 1925, 2028
BsoBI CYCGRG 1 cut(s) 1487
Bsp1286I GDGCHC 2 cut(s) 1901, 2003
Bsp1407I TGTACA 1 cut(s) 453
BspACI CCGC 3 cut(s) 381, 431, 1985
BspANI GGCC 4 cut(s) 784, 1539, 1925, 2028
BspCNI CTCAG 2 cut(s) 520, 802
BspDI ATCGAT 1 cut(s) 2035
BspLI GGNNCC 6 cut(s) 642, 927, 1278, 1633, 1796, 1924
BspMAI CTGCAG 1 cut(s) 1426
BspMI ACCTGC 3 cut(s) 107, 119, 1595
BspPI GGATC 4 cut(s) 158, 635, 648, 1019
BspT107I GGYRCC 1 cut(s) 1276
BsrDI GCAATG 2 cut(s) 214, 364
BsrGI TGTACA 1 cut(s) 453
BsrI ACTGG 3 cut(s) 722, 1633, 1777
BssECI CCNNGG 3 cut(s) 886, 887, 1926
Bst2UI CCWGG 4 cut(s) 461, 888, 930, 1927
Bst4CI ACNGT 2 cut(s) 406, 1554
Bst6I CTCTTC 3 cut(s) 417, 1109, 1128
BstAUI TGTACA 1 cut(s) 453
BstC8I GCNNGC 2 cut(s) 1494, 1807
BstDEI CTNAG 2 cut(s) 507, 810
BstF5I GGATG 3 cut(s) 1193, 1303, 1449
BstH2I RGCGCY 1 cut(s) 2046
BstHHI GCGC 2 cut(s) 1091, 2045
BstMAI GTCTC 2 cut(s) 351, 1046
BstMWI GCNNNNNNNGC 4 cut(s) 155, 387, 851, 1086
BstNI CCWGG 4 cut(s) 461, 888, 930, 1927
BstNSI RCATGY 3 cut(s) 665, 845, 1350
BstSCI CCNGG 4 cut(s) 459, 886, 928, 1925
BstSFI CTRYAG 1 cut(s) 1422
BstV1I GCAGC 2 cut(s) 241, 866
BstV2I GAAGAC 2 cut(s) 1646, 1772
BstX2I RGATCY 3 cut(s) 640, 1024, 1124
BstXI CCANNNNNNTGG 1 cut(s) 1367
BstYI RGATCY 3 cut(s) 640, 1024, 1124
Bsu15I ATCGAT 1 cut(s) 2035
BsuI GTATCC 2 cut(s) 132, 181
BsuRI GGCC 4 cut(s) 784, 1539, 1925, 2028
BsuTUI ATCGAT 1 cut(s) 2035
BtsCI GGATG 3 cut(s) 1193, 1303, 1449
BtsIMutI CAGTG 1 cut(s) 729
BveI ACCTGC 3 cut(s) 107, 119, 1595
Cac8I GCNNGC 2 cut(s) 1494, 1807
CaiI CAGNNNCTG 1 cut(s) 881
CfoI GCGC 2 cut(s) 1091, 2045
Cfr13I GGNCC 4 cut(s) 872, 1795, 1844, 1923
ClaI ATCGAT 1 cut(s) 2035
Csp6I GTAC 1 cut(s) 454
CspCI CAANNNNNGTGG 2 cut(s) 223, 258
CviQI GTAC 1 cut(s) 454
DdeI CTNAG 2 cut(s) 507, 810
DraIII CACNNNGTG 1 cut(s) 724
DriI GACNNNNNGTC 1 cut(s) 474
Eam1104I CTCTTC 3 cut(s) 417, 1109, 1128
Eam1105I GACNNNNNGTC 1 cut(s) 474
EarI CTCTTC 3 cut(s) 417, 1109, 1128
Ecl136II GAGCTC 1 cut(s) 1899
Eco147I AGGCCT 1 cut(s) 784
Eco24I GRGCYC 2 cut(s) 1901, 2003
Eco32I GATATC 2 cut(s) 577, 1233
Eco47I GGWCC 3 cut(s) 872, 1795, 1844
Eco53kI GAGCTC 1 cut(s) 1899
Eco57I CTGAAG 3 cut(s) 726, 1149, 1962
Eco88I CYCGRG 1 cut(s) 1487
EcoICRI GAGCTC 1 cut(s) 1899
EcoO109I RGGNCCY 1 cut(s) 1795
EcoRII CCWGG 4 cut(s) 459, 886, 928, 1925
EcoRV GATATC 2 cut(s) 577, 1233
EcoT22I ATGCAT 2 cut(s) 86, 987
EcoT38I GRGCYC 2 cut(s) 1901, 2003
FalI AAGNNNNNCTT 2 cut(s) 724, 756
FaqI GGGAC 5 cut(s) 69, 481, 734, 869, 1808
FauI CCCGC 1 cut(s) 374
FauNDI CATATG 1 cut(s) 981
FbaI TGATCA 3 cut(s) 676, 907, 1309
Fnu4HI GCNGC 4 cut(s) 255, 432, 855, 1986
FokI GGATG 3 cut(s) 1180, 1290, 1456
FriOI GRGCYC 2 cut(s) 1901, 2003
Fsp4HI GCNGC 4 cut(s) 255, 432, 855, 1986
FspBI CTAG 3 cut(s) 59, 797, 1746
GlaI GCGC 2 cut(s) 1090, 2044
GluI GCNGC 4 cut(s) 255, 432, 855, 1986
HaeII RGCGCY 1 cut(s) 2046
HaeIII GGCC 4 cut(s) 784, 1539, 1925, 2028
HhaI GCGC 2 cut(s) 1091, 2045
Hin6I GCGC 2 cut(s) 1089, 2043
HinP1I GCGC 2 cut(s) 1089, 2043
HincII GTYRAC 1 cut(s) 1827
HindII GTYRAC 1 cut(s) 1827
HindIII AAGCTT 2 cut(s) 738, 1807
HphI GGTGA 8 cut(s) 116, 213, 569, 686, 710, 769, 1255, 1787
Hpy166II GTNNAC 5 cut(s) 718, 727, 1101, 1712, 1827
Hpy8I GTNNAC 5 cut(s) 718, 727, 1101, 1712, 1827
HpyAV CCTTC 3 cut(s) 1240, 1785, 1808
HpyCH4III ACNGT 2 cut(s) 406, 1554
HpyCH4IV ACGT 4 cut(s) 414, 1103, 1339, 1527
HpyF10VI GCNNNNNNNGC 4 cut(s) 155, 387, 851, 1086
HpyF3I CTNAG 2 cut(s) 507, 810
HpySE526I ACGT 4 cut(s) 414, 1103, 1339, 1527
HspAI GCGC 2 cut(s) 1089, 2043
Ksp22I TGATCA 3 cut(s) 676, 907, 1309
LmnI GCTCC 3 cut(s) 1220, 1904, 1930
Lsp1109I GCAGC 2 cut(s) 241, 866
LweI GCATC 1 cut(s) 994
MaeI CTAG 3 cut(s) 59, 797, 1746
MaeII ACGT 4 cut(s) 414, 1103, 1339, 1527
MaeIII GTNAC 6 cut(s) 43, 211, 410, 557, 1451, 1964
MflI RGATCY 3 cut(s) 640, 1024, 1124
MhlI GDGCHC 2 cut(s) 1901, 2003
MlyI GAGTC 2 cut(s) 868, 1822
MmeI TCCRAC 2 cut(s) 862, 1893
Mph1103I ATGCAT 2 cut(s) 86, 987
MroXI GAANNNNTTC 1 cut(s) 942
MseI TTAA 4 cut(s) 821, 894, 1530, 1853
MslI CAYNNNNRTG 3 cut(s) 666, 1068, 1317
MspA1I CMGCKG 1 cut(s) 809
MspR9I CCNGG 4 cut(s) 461, 888, 930, 1927
Mva1269I GAATGC 3 cut(s) 84, 149, 1044
MvaI CCWGG 4 cut(s) 461, 888, 930, 1927
MwoI GCNNNNNNNGC 4 cut(s) 155, 387, 851, 1086
NdeI CATATG 1 cut(s) 981
NlaIV GGNNCC 6 cut(s) 642, 927, 1278, 1633, 1796, 1924
NmuCI GTSAC 4 cut(s) 43, 211, 557, 1451
NsiI ATGCAT 2 cut(s) 86, 987
NspI RCATGY 3 cut(s) 665, 845, 1350
PaeR7I CTCGAG 1 cut(s) 1487
PaqCI CACCTGC 1 cut(s) 107
PasI CCCWGGG 1 cut(s) 887
PceI AGGCCT 1 cut(s) 784
PciI ACATGT 1 cut(s) 1346
PcsI WCGNNNNNNNCGW 1 cut(s) 159
PctI GAATGC 3 cut(s) 84, 149, 1044
PdmI GAANNNNTTC 1 cut(s) 942
PflFI GACNNNGTC 1 cut(s) 343
PfoI TCCNGGA 1 cut(s) 928
PkrI GCNGC 4 cut(s) 256, 433, 856, 1987
PleI GAGTC 2 cut(s) 867, 1822
PpsI GAGTC 2 cut(s) 867, 1822
PpuMI RGGWCCY 1 cut(s) 1795
PscI ACATGT 1 cut(s) 1346
PsiI TTATAA 2 cut(s) 287, 1671
Psp124BI GAGCTC 1 cut(s) 1901
Psp1406I AACGTT 1 cut(s) 1527
Psp5II RGGWCCY 1 cut(s) 1795
Psp6I CCWGG 4 cut(s) 459, 886, 928, 1925
PspGI CCWGG 4 cut(s) 459, 886, 928, 1925
PspN4I GGNNCC 6 cut(s) 642, 927, 1278, 1633, 1796, 1924
PspPI GGNCC 4 cut(s) 872, 1795, 1844, 1923
PspPPI RGGWCCY 1 cut(s) 1795
PspXI VCTCGAGB 1 cut(s) 1487
PstI CTGCAG 1 cut(s) 1426
PstNI CAGNNNCTG 1 cut(s) 881
PsuI RGATCY 3 cut(s) 640, 1024, 1124
PsyI GACNNNGTC 1 cut(s) 343
PvuII CAGCTG 1 cut(s) 809
RsaI GTAC 1 cut(s) 455
RsaNI GTAC 1 cut(s) 454
RseI CAYNNNNRTG 3 cut(s) 666, 1068, 1317
SacI GAGCTC 1 cut(s) 1901
SaqAI TTAA 4 cut(s) 821, 894, 1530, 1853
SatI GCNGC 4 cut(s) 255, 432, 855, 1986
Sau96I GGNCC 4 cut(s) 872, 1795, 1844, 1923
SchI GAGTC 2 cut(s) 868, 1822
ScrFI CCNGG 4 cut(s) 461, 888, 930, 1927
SduI GDGCHC 2 cut(s) 1901, 2003
SfaNI GCATC 1 cut(s) 994
SfcI CTRYAG 1 cut(s) 1422
Sfr274I CTCGAG 1 cut(s) 1487
SinI GGWCC 3 cut(s) 872, 1795, 1844
SlaI CTCGAG 1 cut(s) 1487
SmiMI CAYNNNNRTG 3 cut(s) 666, 1068, 1317
SmlI CTYRAG 2 cut(s) 1487, 1892
SmoI CTYRAG 2 cut(s) 1487, 1892
SpeI ACTAGT 1 cut(s) 58
SseBI AGGCCT 1 cut(s) 784
SsiI CCGC 3 cut(s) 381, 431, 1985
SspMI CTAG 3 cut(s) 59, 797, 1746
SstI GAGCTC 1 cut(s) 1901
StuI AGGCCT 1 cut(s) 784
StyD4I CCNGG 4 cut(s) 459, 886, 928, 1925
TaaI ACNGT 2 cut(s) 406, 1554
TaiI ACGT 4 cut(s) 417, 1106, 1342, 1530
TaqI TCGA 6 cut(s) 489, 588, 1074, 1488, 1731, 2035
TatI WGTACW 1 cut(s) 453
TauI GCSGC 2 cut(s) 434, 1988
Tru1I TTAA 4 cut(s) 821, 894, 1530, 1853
Tru9I TTAA 4 cut(s) 821, 894, 1530, 1853
TscAI CASTG 1 cut(s) 729
TseFI GTSAC 4 cut(s) 43, 211, 557, 1451
TseI GCWGC 2 cut(s) 254, 854
Tsp45I GTSAC 4 cut(s) 43, 211, 557, 1451
TspDTI ATGAA 9 cut(s) 513, 523, 639, 666, 707, 1129, 1238, 1569, 1605
TspGWI ACGGA 2 cut(s) 1456, 1496
TspRI CASTG 1 cut(s) 729
Tth111I GACNNNGTC 1 cut(s) 343
VpaK11BI GGWCC 3 cut(s) 872, 1795, 1844
XapI RAATTY 6 cut(s) 320, 529, 1006, 1170, 1544, 1778
XceI RCATGY 3 cut(s) 665, 845, 1350
XhoI CTCGAG 1 cut(s) 1487
XmnI GAANNNNTTC 1 cut(s) 942
XspI CTAG 3 cut(s) 59, 797, 1746
Zsp2I ATGCAT 2 cut(s) 86, 987
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.