pycom16g19820

Domain of unknown function (DUF4487)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0006689
Physical Location & Seq
Forward (+)
17226324 .. 17228699
2376 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 771 bp
ATGTTAAACAAGGCTATATTACATGTGGCTGTAAAATACGTAGAATCTGATATATCCAATGCTCTAGCACATTTCCTTGCCCTCGGGATCAAGGTAATATGTCTTTTCGTGCAGTGTGCCTGTATTAAAATCCAGGATGCTAGCATATGGTGTGTTAAGCACCTAAAAATGACTCTTATGTCAAGTGAGGAGTCTCAAGAGGAAGAGCACGGCAATCTGTTCGAGAGAATTCGGTCATTTGAATCAGTAGGAAGGGTCACATTGGAGGTCATTGATGCTGTAATAAGAATATGTGGAGCATATGCTCGAGCTGTAAATTGGGAGTCTTGGGAAGACAAGCTTGAGATGGATAAATCTGGAATGGAATTTGAAGGGGTCAGCAATATGAATCACGTTATTAATGTAATAAAGTACACAATAGAAAAATTGTGTGAGATAGGCGTTGTTGCTGCTAATAATGGGGGCTGTCTGGTAAAGGTTCTCAATTTTTCGTGGAAAGGTGTAGTCACTTTGCTTCAGCTTGGTGAGGGGGTATGCGCAACAAAGGTGAATGTAGCCAATATAATTTCAAATCTTGTCTCATTGGTCAATGAATCTTTAAGATGTGCAGCTGAGGCTTGGTCATCATCTTTGAATGAAACCATCTCCGTGACAGAAGCTAGAAAGACATTTCTTCCAGTAAAGTTTTATCTGATTAATGCAATAAAAATATCTACCATGTATCCATTTCAAGCATGTCTGGTACAGAGGGAGATAACAAATGCATCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4487

Protein Analysis

257

Amino Acids

28.41

Weight (kDa)

6.0

Isoelectric Point (pI)

37.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4487 PF14868 159 - 253 1.1e-26 Domain of unknown function (DUF4487)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 178
Acc16I TGCGCA 1 cut(s) 538
AclWI GGATC 1 cut(s) 95
AcsI RAATTY 2 cut(s) 228, 365
AcuI CTGAAG 1 cut(s) 500
AfaI GTAC 2 cut(s) 413, 744
AflIII ACRYGT 1 cut(s) 22
AgsI TTSAA 5 cut(s) 242, 371, 570, 634, 731
AjnI CCWGG 1 cut(s) 132
AluBI AGCT 5 cut(s) 311, 340, 520, 611, 659
AluI AGCT 5 cut(s) 311, 340, 520, 611, 659
Alw21I GWGCWC 1 cut(s) 210
Alw26I GTCTC 2 cut(s) 198, 583
AlwI GGATC 1 cut(s) 95
Ama87I CYCGRG 2 cut(s) 83, 306
ApeKI GCWGC 2 cut(s) 449, 608
ApoI RAATTY 2 cut(s) 228, 365
AseI ATTAAT 2 cut(s) 399, 696
AspLEI GCGC 1 cut(s) 539
AsuHPI GGTGA 2 cut(s) 536, 559
AsuNHI GCTAGC 1 cut(s) 140
AvaI CYCGRG 2 cut(s) 83, 306
BbsI GAAGAC 1 cut(s) 339
Bbv12I GWGCWC 1 cut(s) 210
BbvCI CCTCAGC 1 cut(s) 612
BbvI GCAGC 2 cut(s) 436, 620
BccI CCATC 2 cut(s) 340, 650
BceAI ACGGC 1 cut(s) 226
BcgI CGANNNNNNTGC 2 cut(s) 202, 236
BciT130I CCWGG 1 cut(s) 134
BciVI GTATCC 1 cut(s) 732
BcoDI GTCTC 2 cut(s) 198, 583
BfaI CTAG 3 cut(s) 65, 141, 660
BfuI GTATCC 1 cut(s) 732
BisI GCNGC 2 cut(s) 450, 609
BlsI GCNGC 2 cut(s) 451, 610
Bme1390I CCNGG 1 cut(s) 134
BmeT110I CYCGRG 2 cut(s) 83, 306
BmrFI CCNGG 1 cut(s) 134
BmsI GCATC 2 cut(s) 127, 265
BmtI GCTAGC 1 cut(s) 144
BpiI GAAGAC 1 cut(s) 339
Bpu10I CCTNAGC 1 cut(s) 612
BpuEI CTTGAG 2 cut(s) 180, 362
BsaAI YACGTR 1 cut(s) 40
BsaJI CCNNGG 1 cut(s) 82
Bse1I ACTGG 1 cut(s) 677
BseBI CCWGG 1 cut(s) 134
BseDI CCNNGG 1 cut(s) 82
BseGI GGATG 2 cut(s) 142, 764
BseMII CTCAG 1 cut(s) 603
BseNI ACTGG 1 cut(s) 677
BseRI GAGGAG 1 cut(s) 203
BseXI GCAGC 2 cut(s) 436, 620
BsgI GTGCAG 2 cut(s) 131, 627
BsiHKAI GWGCWC 1 cut(s) 210
BsiHKCI CYCGRG 2 cut(s) 83, 306
BsmAI GTCTC 2 cut(s) 198, 583
BsoBI CYCGRG 2 cut(s) 83, 306
Bsp1286I GDGCHC 1 cut(s) 210
Bsp143I GATC 1 cut(s) 87
BspCNI CTCAG 1 cut(s) 604
BspOI GCTAGC 1 cut(s) 144
BspPI GGATC 1 cut(s) 95
BspQI GCTCTTC 1 cut(s) 198
BsrI ACTGG 1 cut(s) 677
BssECI CCNNGG 1 cut(s) 82
BssMI GATC 1 cut(s) 87
Bst2UI CCWGG 1 cut(s) 134
Bst6I CTCTTC 1 cut(s) 198
BstBAI YACGTR 1 cut(s) 40
BstC8I GCNNGC 1 cut(s) 142
BstDEI CTNAG 1 cut(s) 612
BstF5I GGATG 2 cut(s) 142, 764
BstHHI GCGC 1 cut(s) 539
BstKTI GATC 1 cut(s) 90
BstMAI GTCTC 2 cut(s) 198, 583
BstMBI GATC 1 cut(s) 87
BstMWI GCNNNNNNNGC 1 cut(s) 614
BstNI CCWGG 1 cut(s) 134
BstNSI RCATGY 2 cut(s) 26, 738
BstSCI CCNGG 1 cut(s) 132
BstSNI TACGTA 1 cut(s) 40
BstV1I GCAGC 2 cut(s) 436, 620
BstV2I GAAGAC 1 cut(s) 339
BsuI GTATCC 1 cut(s) 732
BtsCI GGATG 2 cut(s) 142, 764
BtsI GCAGTG 1 cut(s) 119
BtsIMutI CAGTG 1 cut(s) 119
Cac8I GCNNGC 1 cut(s) 142
CfoI GCGC 1 cut(s) 539
Csp6I GTAC 2 cut(s) 412, 743
CviAII CATG 3 cut(s) 23, 718, 735
CviQI GTAC 2 cut(s) 412, 743
DdeI CTNAG 1 cut(s) 612
DpnI GATC 1 cut(s) 89
DpnII GATC 1 cut(s) 87
DrdI GACNNNNNNGTC 1 cut(s) 178
DseDI GACNNNNNNGTC 1 cut(s) 178
Eam1104I CTCTTC 1 cut(s) 198
EarI CTCTTC 1 cut(s) 198
Eco105I TACGTA 1 cut(s) 40
Eco57I CTGAAG 1 cut(s) 500
Eco88I CYCGRG 2 cut(s) 83, 306
EcoRI GAATTC 1 cut(s) 228
EcoRII CCWGG 1 cut(s) 132
EcoT22I ATGCAT 1 cut(s) 766
FaeI CATG 3 cut(s) 26, 721, 738
FalI AAGNNNNNCTT 2 cut(s) 324, 356
FatI CATG 3 cut(s) 22, 717, 734
FauNDI CATATG 2 cut(s) 146, 301
Fnu4HI GCNGC 2 cut(s) 450, 609
FokI GGATG 2 cut(s) 149, 751
Fsp4HI GCNGC 2 cut(s) 450, 609
FspBI CTAG 3 cut(s) 65, 141, 660
FspI TGCGCA 1 cut(s) 538
GlaI GCGC 1 cut(s) 538
GluI GCNGC 2 cut(s) 450, 609
HhaI GCGC 1 cut(s) 539
Hin1II CATG 3 cut(s) 26, 721, 738
Hin6I GCGC 1 cut(s) 537
HinP1I GCGC 1 cut(s) 537
HindIII AAGCTT 1 cut(s) 338
HinfI GANTC 7 cut(s) 44, 172, 191, 242, 323, 388, 593
HphI GGTGA 2 cut(s) 536, 559
Hpy166II GTNNAC 1 cut(s) 414
Hpy188I TCNGA 2 cut(s) 49, 693
Hpy188III TCNNGA 5 cut(s) 85, 197, 223, 357, 768
Hpy8I GTNNAC 1 cut(s) 414
HpyAV CCTTC 2 cut(s) 246, 365
HpyCH4IV ACGT 2 cut(s) 39, 393
HpyCH4V TGCA 4 cut(s) 112, 608, 701, 764
HpyF10VI GCNNNNNNNGC 1 cut(s) 614
HpyF3I CTNAG 1 cut(s) 612
HpySE526I ACGT 2 cut(s) 39, 393
Hsp92II CATG 3 cut(s) 26, 721, 738
HspAI GCGC 1 cut(s) 537
Kzo9I GATC 1 cut(s) 87
LguI GCTCTTC 1 cut(s) 198
LmnI GCTCC 1 cut(s) 296
LpnPI CCDG 7 cut(s) 119, 133, 146, 342, 455, 690, 725
Lsp1109I GCAGC 2 cut(s) 436, 620
LweI GCATC 2 cut(s) 127, 265
MaeI CTAG 3 cut(s) 65, 141, 660
MaeII ACGT 2 cut(s) 39, 393
MaeIII GTNAC 3 cut(s) 256, 505, 649
MalI GATC 1 cut(s) 89
MboI GATC 1 cut(s) 87
MboII GAAGA 3 cut(s) 215, 344, 665
MhlI GDGCHC 1 cut(s) 210
MluCI AATT 6 cut(s) 228, 316, 365, 425, 484, 564
MlyI GAGTC 3 cut(s) 166, 200, 332
MnlI CCTC 7 cut(s) 92, 181, 193, 259, 520, 607, 741
Mph1103I ATGCAT 1 cut(s) 766
MseI TTAA 6 cut(s) 5, 126, 156, 399, 599, 696
MslI CAYNNNNRTG 1 cut(s) 647
MspA1I CMGCKG 1 cut(s) 611
MspR9I CCNGG 1 cut(s) 134
MvaI CCWGG 1 cut(s) 134
MwoI GCNNNNNNNGC 1 cut(s) 614
NdeI CATATG 2 cut(s) 146, 301
NdeII GATC 1 cut(s) 87
NheI GCTAGC 1 cut(s) 140
NlaIII CATG 3 cut(s) 26, 721, 738
NmuCI GTSAC 3 cut(s) 256, 505, 649
NsbI TGCGCA 1 cut(s) 538
NsiI ATGCAT 1 cut(s) 766
NspI RCATGY 2 cut(s) 26, 738
PaeR7I CTCGAG 1 cut(s) 306
PciI ACATGT 1 cut(s) 22
PciSI GCTCTTC 1 cut(s) 198
PfeI GAWTC 4 cut(s) 44, 242, 388, 593
PfoI TCCNGGA 1 cut(s) 132
PkrI GCNGC 2 cut(s) 451, 610
PleI GAGTC 3 cut(s) 166, 199, 331
PpsI GAGTC 3 cut(s) 166, 199, 331
Ppu21I YACGTR 1 cut(s) 40
PscI ACATGT 1 cut(s) 22
PshBI ATTAAT 2 cut(s) 399, 696
Psp6I CCWGG 1 cut(s) 132
PspGI CCWGG 1 cut(s) 132
PspXI VCTCGAGB 1 cut(s) 306
PvuII CAGCTG 1 cut(s) 611
RsaI GTAC 2 cut(s) 413, 744
RsaNI GTAC 2 cut(s) 412, 743
RseI CAYNNNNRTG 1 cut(s) 647
SapI GCTCTTC 1 cut(s) 198
SaqAI TTAA 6 cut(s) 5, 126, 156, 399, 599, 696
SatI GCNGC 2 cut(s) 450, 609
Sau3AI GATC 1 cut(s) 87
SchI GAGTC 3 cut(s) 166, 200, 332
ScrFI CCNGG 1 cut(s) 134
SduI GDGCHC 1 cut(s) 210
SfaNI GCATC 2 cut(s) 127, 265
Sfr274I CTCGAG 1 cut(s) 306
SlaI CTCGAG 1 cut(s) 306
SmiMI CAYNNNNRTG 1 cut(s) 647
SmlI CTYRAG 3 cut(s) 195, 306, 341
SmoI CTYRAG 3 cut(s) 195, 306, 341
SnaBI TACGTA 1 cut(s) 40
Sse9I AATT 6 cut(s) 228, 316, 365, 425, 484, 564
SspMI CTAG 3 cut(s) 65, 141, 660
StyD4I CCNGG 1 cut(s) 132
TaiI ACGT 2 cut(s) 42, 396
TaqI TCGA 2 cut(s) 222, 307
TaqII GACCGA 1 cut(s) 222
TasI AATT 6 cut(s) 228, 316, 365, 425, 484, 564
TatI WGTACW 1 cut(s) 411
TfiI GAWTC 4 cut(s) 44, 242, 388, 593
Tru1I TTAA 6 cut(s) 5, 126, 156, 399, 599, 696
Tru9I TTAA 6 cut(s) 5, 126, 156, 399, 599, 696
TscAI CASTG 1 cut(s) 119
TseFI GTSAC 3 cut(s) 256, 505, 649
TseI GCWGC 2 cut(s) 449, 608
Tsp45I GTSAC 3 cut(s) 256, 505, 649
TspDTI ATGAA 3 cut(s) 401, 606, 651
TspGWI ACGGA 1 cut(s) 637
TspRI CASTG 1 cut(s) 119
VspI ATTAAT 2 cut(s) 399, 696
XapI RAATTY 2 cut(s) 228, 365
XceI RCATGY 2 cut(s) 26, 738
XhoI CTCGAG 1 cut(s) 306
XspI CTAG 3 cut(s) 65, 141, 660
Zsp2I ATGCAT 1 cut(s) 766
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.