Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0002359

Physical Location & Seq

Genomic Coordinates Forward (+)

18866277 .. 18868968

Gene Structure 2692 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom16g20820.3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1302 bp

ATGTCTTCGTGTCTAAGAGGCGGCGGAAGAACCTATGCCTTCGAGCTTGACATAGTCAAATCGCCGTCCACATCAACCTTCACCTCCACCTCTTCTCCGTCCTCAACTCTCTCGGAATCCAGCAACTCCGGCCTCGCAATCTCGACGAGAAAACCTCGTACCCCTCGAAAACGGCCAAACCAAACATACAACGAAGCCGCCGCATTGCTCTCCACAGCGTACCCCAACATCTTCTCTTCCAAGAACTTCACAAATCCCAGAAAATTCACTAAACCCCACGACTCTTTCTTCGACCAATCCCCAGAATTGCTCCTGCCCTTCAGAGTCATCGAAGCCGAAAATGCCGGGTTCTTGCTCCACCTCCCAATCCGGGAAAAACCCAGTTCCCAATTCGAGCCCAAGTCCCTGAATTCGTTCGAGAAGCCGTGCCGGAGTCCCGGGGAATTCGATTCCCAGCCGAATTGCAATTCCGATTCCATGGAGTTGTGCGACGTGTACAATTTTCAAGAGGAAGACTTCGATGCGGAGTCGATTCTCGACGAGGAAATTGAAGAGGGGATTGATAGCATCATGGGAAGTATGAGTGTGAACATGGACTTGGAGGATGAGTCTACAAATGGAGGTGCGCAAATGAATAATTCGAATCCGAACTGGAATTCCTGTTATGGGTACCCAATGGGGCTCGGATTTGGCGGGAAATTCGAATTCGGGTTCGGGTCGAGAAGAGGAGTGAAGCCGTTGAGGCACGTCGATAATGGGAACTGGTGGAGTTTTCCCACCGTCGATGTTCTCGAAATCTCCCCCAGATTCAACAAATCTCAGTCGTGTTCCGCTCCGGCTCCAGCCGCGGCCACAGCCGGGAAGAAGAAGAAGAAGAAGGTGGAGAAGCTGTCGGTGTTGGAAAGGAGAACCGCGGCGGAATTGCCGAAGGAGGCGAATCCGATTCCGAAGCCCCCGGCGGAGGAGGAGCCCGGGCTGATGCTAAAACTGGACTACGACGACGTTTTGAATGCTTGGTCGGACAAAGCGTCGCCGTTTTCGGAGGAAATGCCGGGATCTGATGCAGCGGCGGGGAATGATGTATCTGTCAGGTTGGCGCATATTGATTTATTCTCGGATGTCGGAGGACTGAGGGAGGCTAGCGTGTTGCGCTACAAGGAGAAACGTCGTACCCGTCTCTACTCTAAAAAGATCAGATACCAAGTTAGAAAAGCCAATGCCGATCGACGACCTAGAATGAAGGGAAGATTTGTTAGAAGGCCACATTCTAGCACAAACGTTCAAAGACAAAAGGAAATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006605 GO:0006810 GO:0006886 GO:0008104 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0015031 GO:0015833 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0033036 GO:0033365 GO:0034613 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045036 GO:0045184 GO:0046907 GO:0050789 GO:0050794 GO:0051171 GO:0051179 GO:0051234 GO:0051252 GO:0051641 GO:0051649 GO:0060255 GO:0065007 GO:0070727 GO:0071702 GO:0071705 GO:0072594 GO:0072596 GO:0072598 GO:0080090 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CCT

Protein Analysis

434

Amino Acids

48.34

Weight (kDa)

8.57

Isoelectric Point (pI)

59.46

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002359)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G25990 AT4G25990 AT4G25990 AT5G57180 AT5G57180 AT5G57180 AT5G57180
fragaria_vesca	FvH4_2g01490 FvH4_4g07270 FvH4_4g07270 FvH4_4g07270 FvH4_4g07270 FvH4_4g07270 FvH4_4g07270 FvH4_6g24780 FvH4_6g24780 FvH4_6g24780 FvH4_6g24780 FvH4_6g24780 FvH4_6g24780 FvH4_6g24780
malus_domestica	MD13G1239300.v1.1 MD16G1248400.v1.1
prunus_persica	Prupe.1G074900_v2.0.a1 Prupe.1G074900_v2.0.a1 Prupe.1G074900_v2.0.a1 Prupe.1G074900_v2.0.a1
pyrus_communis	pycom13g21340 pycom16g20820
rosa_chinensis	RchiOBHm_Chr4g0399481
rosa_laevigata	RLG00000009263
rosa_multiflora	Rmu_sc0002285.1_g000032 Rmu_sc0004311.1_g000007
rosa_roxburghii	Rroxscaffold_5G00344410
rosa_rugosa	Rorug04G0017200 Rorug04G0017300 Rorug04G0017300
rosa_samantha	Rh4AG095100 Rh4BG092500 Rh4CG103600 Rh4DG087400
rosa_wichuraiana	Rw4G007820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	627
Acc65I	GGTACC	1 cut(s)	669
AccB1I	GGYRCC	1 cut(s)	669
AccBSI	CCGCTC	1 cut(s)	833
AccI	GTMKAC	1 cut(s)	611
AccII	CGCG	2 cut(s)	848, 914
AclI	AACGTT	1 cut(s)	1278
AclWI	GGATC	1 cut(s)	1063
AcoI	YGGCCR	2 cut(s)	173, 849
AcsI	RAATTY	6 cut(s)	263, 409, 443, 655, 698, 704
AcuI	CTGAAG	1 cut(s)	304
AfaI	GTAC	5 cut(s)	160, 221, 497, 671, 1171
AfiI	CCNNNNNNNGG	4 cut(s)	370, 666, 858, 961
AflIII	ACRYGT	1 cut(s)	492
AgsI	TTSAA	5 cut(s)	506, 551, 811, 1009, 1283
AhdI	GACNNNNNGTC	1 cut(s)	1027
AjiI	CACGTC	2 cut(s)	493, 748
AjuI	GAANNNNNNNTTGG	2 cut(s)	581, 613
AloI	GAACNNNNNNTCC	2 cut(s)	641, 673
AluBI	AGCT	2 cut(s)	46, 889
AluI	AGCT	2 cut(s)	46, 889
Alw26I	GTCTC	1 cut(s)	1181
AlwI	GGATC	1 cut(s)	1063
Ama87I	CYCGRG	2 cut(s)	437, 971
AoxI	GGCC	4 cut(s)	130, 173, 849, 1259
ApeKI	GCWGC	1 cut(s)	1064
ApoI	RAATTY	6 cut(s)	263, 409, 443, 655, 698, 704
ArsI	GACNNNNNNTTYG	2 cut(s)	272, 304
Asp700I	GAANNNNTTC	1 cut(s)	413
Asp718I	GGTACC	1 cut(s)	669
AspLEI	GCGC	3 cut(s)	628, 1099, 1152
AsuC2I	CCSGG	9 cut(s)	346, 371, 438, 439, 859, 956, 972, 973, 1053
AsuHPI	GGTGA	1 cut(s)	73
AsuII	TTCGAA	2 cut(s)	641, 702
AsuNHI	GCTAGC	1 cut(s)	1139
AvaI	CYCGRG	2 cut(s)	437, 971
BanI	GGYRCC	1 cut(s)	669
BanII	GRGCYC	3 cut(s)	399, 684, 972
BbsI	GAAGAC	1 cut(s)	519
BbvI	GCAGC	1 cut(s)	1076
BceAI	ACGGC	5 cut(s)	49, 188, 409, 721, 1018
BcnI	CCSGG	9 cut(s)	346, 371, 438, 439, 859, 956, 972, 973, 1053
BcoDI	GTCTC	1 cut(s)	1181
BfaI	CTAG	3 cut(s)	1140, 1233, 1269
BglI	GCCNNNNNGGC	1 cut(s)	742
BisI	GCNGC	8 cut(s)	22, 198, 201, 846, 849, 915, 1065, 1068
BlsI	GCNGC	8 cut(s)	23, 199, 202, 847, 850, 916, 1066, 1069
Bme1390I	CCNGG	9 cut(s)	346, 371, 438, 439, 859, 956, 972, 973, 1053
BmeRI	GACNNNNNGTC	1 cut(s)	1027
BmeT110I	CYCGRG	2 cut(s)	437, 971
BmgBI	CACGTC	2 cut(s)	493, 748
BmiI	GGNNCC	3 cut(s)	671, 840, 969
BmrFI	CCNGG	9 cut(s)	346, 371, 438, 439, 859, 956, 972, 973, 1053
BmrI	ACTGGG	1 cut(s)	375
BmsI	GCATC	4 cut(s)	511, 576, 969, 1051
BmtI	GCTAGC	1 cut(s)	1143
BmuI	ACTGGG	1 cut(s)	375
BpiI	GAAGAC	1 cut(s)	519
BplI	GAGNNNNNCTC	2 cut(s)	139, 171
BpmI	CTGGAG	1 cut(s)	825
Bpu14I	TTCGAA	2 cut(s)	641, 702
BpuMI	CCSGG	9 cut(s)	346, 371, 438, 439, 859, 956, 972, 973, 1053
BsaJI	CCNNGG	7 cut(s)	437, 438, 477, 846, 912, 954, 971
BsaXI	ACNNNNNCTCC	6 cut(s)	79, 109, 593, 623, 875, 905
Bsc4I	CCNNNNNNNGG	4 cut(s)	370, 666, 858, 961
Bse1I	ACTGG	4 cut(s)	381, 656, 767, 993
Bse3DI	GCAATG	1 cut(s)	203
BseDI	CCNNGG	7 cut(s)	437, 438, 477, 846, 912, 954, 971
BseGI	GGATG	2 cut(s)	610, 1123
BseLI	CCNNNNNNNGG	4 cut(s)	370, 666, 858, 961
BseMI	GCAATG	1 cut(s)	203
BseMII	CTCAG	2 cut(s)	833, 1121
BseNI	ACTGG	4 cut(s)	381, 656, 767, 993
BseRI	GAGGAG	3 cut(s)	741, 977, 980
BseXI	GCAGC	1 cut(s)	1076
BseYI	CCCAGC	1 cut(s)	453
Bsh1236I	CGCG	2 cut(s)	848, 914
Bsh1285I	CGRYCG	1 cut(s)	1225
BshFI	GGCC	4 cut(s)	132, 175, 851, 1261
BshNI	GGYRCC	1 cut(s)	669
BsiEI	CGRYCG	1 cut(s)	1225
BsiHKCI	CYCGRG	2 cut(s)	437, 971
BslFI	GGGAC	2 cut(s)	388, 420
BslI	CCNNNNNNNGG	4 cut(s)	370, 666, 858, 961
BsmAI	GTCTC	1 cut(s)	1181
BsmBI	CGTCTC	1 cut(s)	1181
BsmFI	GGGAC	2 cut(s)	388, 420
BsmI	GAATGC	1 cut(s)	1015
BsnI	GGCC	4 cut(s)	132, 175, 851, 1261
BsoBI	CYCGRG	2 cut(s)	437, 971
Bsp119I	TTCGAA	2 cut(s)	641, 702
Bsp1286I	GDGCHC	3 cut(s)	399, 684, 972
Bsp1407I	TGTACA	1 cut(s)	495
Bsp143I	GATC	3 cut(s)	1055, 1191, 1222
Bsp19I	CCATGG	1 cut(s)	477
BspANI	GGCC	4 cut(s)	132, 175, 851, 1261
BspCNI	CTCAG	2 cut(s)	832, 1122
BspFNI	CGCG	2 cut(s)	848, 914
BspLI	GGNNCC	3 cut(s)	671, 840, 969
BspOI	GCTAGC	1 cut(s)	1143
BspPI	GGATC	1 cut(s)	1063
BspT104I	TTCGAA	2 cut(s)	641, 702
BspT107I	GGYRCC	1 cut(s)	669
BsrBI	CCGCTC	1 cut(s)	833
BsrDI	GCAATG	1 cut(s)	203
BsrGI	TGTACA	1 cut(s)	495
BsrI	ACTGG	4 cut(s)	381, 656, 767, 993
BssECI	CCNNGG	7 cut(s)	437, 438, 477, 846, 912, 954, 971
BssMI	GATC	3 cut(s)	1055, 1191, 1222
BssT1I	CCWWGG	1 cut(s)	477
Bst4CI	ACNGT	1 cut(s)	781
Bst6I	CTCTTC	4 cut(s)	97, 241, 546, 718
BstAUI	TGTACA	1 cut(s)	495
BstBI	TTCGAA	2 cut(s)	641, 702
BstC8I	GCNNGC	1 cut(s)	1141
BstDEI	CTNAG	3 cut(s)	14, 819, 1130
BstDSI	CCRYGG	3 cut(s)	477, 846, 912
BstF5I	GGATG	2 cut(s)	610, 1123
BstFNI	CGCG	2 cut(s)	848, 914
BstHHI	GCGC	3 cut(s)	628, 1099, 1152
BstKTI	GATC	3 cut(s)	1058, 1194, 1225
BstMAI	GTCTC	1 cut(s)	1181
BstMBI	GATC	3 cut(s)	1055, 1191, 1222
BstMCI	CGRYCG	1 cut(s)	1225
BstMWI	GCNNNNNNNGC	6 cut(s)	129, 341, 742, 845, 854, 1149
BstSCI	CCNGG	9 cut(s)	344, 369, 436, 437, 857, 954, 970, 971, 1051
BstUI	CGCG	2 cut(s)	848, 914
BstV1I	GCAGC	1 cut(s)	1076
BstV2I	GAAGAC	1 cut(s)	519
BstX2I	RGATCY	1 cut(s)	1055
BstYI	RGATCY	1 cut(s)	1055
BsuRI	GGCC	4 cut(s)	132, 175, 851, 1261
BtgI	CCRYGG	3 cut(s)	477, 846, 912
BtrI	CACGTC	2 cut(s)	493, 748
BtsCI	GGATG	2 cut(s)	610, 1123
Cac8I	GCNNGC	1 cut(s)	1141
CfoI	GCGC	3 cut(s)	628, 1099, 1152
Cfr42I	CCGCGG	2 cut(s)	849, 915
Cfr9I	CCCGGG	2 cut(s)	437, 971
CseI	GACGC	1 cut(s)	1017
Csp6I	GTAC	5 cut(s)	159, 220, 496, 670, 1170
CviAII	CATG	3 cut(s)	478, 571, 592
CviQI	GTAC	5 cut(s)	159, 220, 496, 670, 1170
DdeI	CTNAG	3 cut(s)	14, 819, 1130
DpnI	GATC	3 cut(s)	1057, 1193, 1224
DpnII	GATC	3 cut(s)	1055, 1191, 1222
DriI	GACNNNNNGTC	1 cut(s)	1027
EaeI	YGGCCR	2 cut(s)	173, 849
Eam1104I	CTCTTC	4 cut(s)	97, 241, 546, 718
Eam1105I	GACNNNNNGTC	1 cut(s)	1027
EarI	CTCTTC	4 cut(s)	97, 241, 546, 718
EciI	GGCGGA	3 cut(s)	39, 932, 974
Eco130I	CCWWGG	1 cut(s)	477
Eco24I	GRGCYC	3 cut(s)	399, 684, 972
Eco57I	CTGAAG	1 cut(s)	304
Eco88I	CYCGRG	2 cut(s)	437, 971
EcoRI	GAATTC	4 cut(s)	409, 443, 655, 704
EcoT14I	CCWWGG	1 cut(s)	477
EcoT38I	GRGCYC	3 cut(s)	399, 684, 972
ErhI	CCWWGG	1 cut(s)	477
Esp3I	CGTCTC	1 cut(s)	1181
FaeI	CATG	3 cut(s)	481, 574, 595
FaqI	GGGAC	2 cut(s)	388, 420
FatI	CATG	3 cut(s)	477, 570, 591
FauI	CCCGC	2 cut(s)	686, 1063
FblI	GTMKAC	1 cut(s)	611
Fnu4HI	GCNGC	8 cut(s)	22, 198, 201, 846, 849, 915, 1065, 1068
FokI	GGATG	2 cut(s)	617, 1130
FriOI	GRGCYC	3 cut(s)	399, 684, 972
Fsp4HI	GCNGC	8 cut(s)	22, 198, 201, 846, 849, 915, 1065, 1068
FspBI	CTAG	3 cut(s)	1140, 1233, 1269
FspI	TGCGCA	1 cut(s)	627
GlaI	GCGC	3 cut(s)	627, 1098, 1151
GluI	GCNGC	8 cut(s)	22, 198, 201, 846, 849, 915, 1065, 1068
GsaI	CCCAGC	1 cut(s)	457
GsuI	CTGGAG	1 cut(s)	825
HaeIII	GGCC	4 cut(s)	132, 175, 851, 1261
HgaI	GACGC	1 cut(s)	1017
HhaI	GCGC	3 cut(s)	628, 1099, 1152
Hin1II	CATG	3 cut(s)	481, 574, 595
Hin6I	GCGC	3 cut(s)	626, 1097, 1150
HinP1I	GCGC	3 cut(s)	626, 1097, 1150
HphI	GGTGA	1 cut(s)	73
Hpy166II	GTNNAC	4 cut(s)	69, 496, 589, 612
Hpy188III	TCNNGA	6 cut(s)	142, 418, 506, 536, 720, 791
Hpy8I	GTNNAC	4 cut(s)	69, 496, 589, 612
HpyAV	CCTTC	7 cut(s)	49, 88, 328, 871, 922, 1234, 1251
HpyCH4III	ACNGT	1 cut(s)	781
HpyCH4IV	ACGT	5 cut(s)	492, 747, 1002, 1165, 1278
HpyCH4V	TGCA	2 cut(s)	465, 1064
HpyF10VI	GCNNNNNNNGC	6 cut(s)	129, 341, 742, 845, 854, 1149
HpyF3I	CTNAG	3 cut(s)	14, 819, 1130
HpySE526I	ACGT	5 cut(s)	492, 747, 1002, 1165, 1278
Hsp92II	CATG	3 cut(s)	481, 574, 595
HspAI	GCGC	3 cut(s)	626, 1097, 1150
KpnI	GGTACC	1 cut(s)	673
KspI	CCGCGG	2 cut(s)	849, 915
Kzo9I	GATC	3 cut(s)	1055, 1191, 1222
LmnI	GCTCC	5 cut(s)	315, 360, 838, 844, 967
Lsp1109I	GCAGC	1 cut(s)	1076
LweI	GCATC	4 cut(s)	511, 576, 969, 1051
MaeI	CTAG	3 cut(s)	1140, 1233, 1269
MaeII	ACGT	5 cut(s)	492, 747, 1002, 1165, 1278
MalI	GATC	3 cut(s)	1057, 1193, 1224
MbiI	CCGCTC	1 cut(s)	833
MboI	GATC	3 cut(s)	1055, 1191, 1222
MflI	RGATCY	1 cut(s)	1055
MhlI	GDGCHC	3 cut(s)	399, 684, 972
MlyI	GAGTC	5 cut(s)	275, 333, 442, 536, 617
MmeI	TCCRAC	3 cut(s)	879, 999, 1102
MroXI	GAANNNNTTC	1 cut(s)	413
MspA1I	CMGCKG	3 cut(s)	848, 914, 1067
MspR9I	CCNGG	9 cut(s)	346, 371, 438, 439, 859, 956, 972, 973, 1053
Mva1269I	GAATGC	1 cut(s)	1015
MvnI	CGCG	2 cut(s)	848, 914
MwoI	GCNNNNNNNGC	6 cut(s)	129, 341, 742, 845, 854, 1149
NciI	CCSGG	9 cut(s)	346, 371, 438, 439, 859, 956, 972, 973, 1053
NcoI	CCATGG	1 cut(s)	477
NdeII	GATC	3 cut(s)	1055, 1191, 1222
NheI	GCTAGC	1 cut(s)	1139
NlaIII	CATG	3 cut(s)	481, 574, 595
NlaIV	GGNNCC	3 cut(s)	671, 840, 969
NsbI	TGCGCA	1 cut(s)	627
NspV	TTCGAA	2 cut(s)	641, 702
PcsI	WCGNNNNNNNCGW	4 cut(s)	163, 789, 1025, 1171
PctI	GAATGC	1 cut(s)	1015
PdmI	GAANNNNTTC	1 cut(s)	413
PfeI	GAWTC	8 cut(s)	116, 449, 473, 532, 643, 807, 937, 943
PflFI	GACNNNGTC	1 cut(s)	53
PfoI	TCCNGGA	1 cut(s)	369
PkrI	GCNGC	8 cut(s)	23, 199, 202, 847, 850, 916, 1066, 1069
Ple19I	CGATCG	1 cut(s)	1225
PleI	GAGTC	5 cut(s)	275, 332, 441, 535, 616
PpsI	GAGTC	5 cut(s)	275, 332, 441, 535, 616
Psp1406I	AACGTT	1 cut(s)	1278
PspFI	CCCAGC	1 cut(s)	453
PspN4I	GGNNCC	3 cut(s)	671, 840, 969
PsuI	RGATCY	1 cut(s)	1055
PsyI	GACNNNGTC	1 cut(s)	53
PvuI	CGATCG	1 cut(s)	1225
RsaI	GTAC	5 cut(s)	160, 221, 497, 671, 1171
RsaNI	GTAC	5 cut(s)	159, 220, 496, 670, 1170
SacII	CCGCGG	2 cut(s)	849, 915
SatI	GCNGC	8 cut(s)	22, 198, 201, 846, 849, 915, 1065, 1068
Sau3AI	GATC	3 cut(s)	1055, 1191, 1222
SchI	GAGTC	5 cut(s)	275, 333, 442, 536, 617
ScrFI	CCNGG	9 cut(s)	346, 371, 438, 439, 859, 956, 972, 973, 1053
SduI	GDGCHC	3 cut(s)	399, 684, 972
SfaNI	GCATC	4 cut(s)	511, 576, 969, 1051
Sfr303I	CCGCGG	2 cut(s)	849, 915
SfuI	TTCGAA	2 cut(s)	641, 702
SgrBI	CCGCGG	2 cut(s)	849, 915
SmaI	CCCGGG	2 cut(s)	439, 973
SrfI	GCCCGGGC	1 cut(s)	973
SspMI	CTAG	3 cut(s)	1140, 1233, 1269
StyD4I	CCNGG	9 cut(s)	344, 369, 436, 437, 857, 954, 970, 971, 1051
StyI	CCWWGG	1 cut(s)	477
TaaI	ACNGT	1 cut(s)	781
TaiI	ACGT	5 cut(s)	495, 750, 1005, 1168, 1281
TatI	WGTACW	1 cut(s)	495
TauI	GCSGC	7 cut(s)	24, 200, 203, 848, 851, 917, 1070
TfiI	GAWTC	8 cut(s)	116, 449, 473, 532, 643, 807, 937, 943
TseI	GCWGC	1 cut(s)	1064
TspDTI	ATGAA	2 cut(s)	647, 1253
TspGWI	ACGGA	1 cut(s)	87
TspMI	CCCGGG	2 cut(s)	437, 971
Tth111I	GACNNNGTC	1 cut(s)	53
XapI	RAATTY	6 cut(s)	263, 409, 443, 655, 698, 704
XmaI	CCCGGG	2 cut(s)	437, 971
XmiI	GTMKAC	1 cut(s)	611
XmnI	GAANNNNTTC	1 cut(s)	413
XspI	CTAG	3 cut(s)	1140, 1233, 1269

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.